WT/EE QL QL/EE XTP GT FLAG HA 3XHA WT 3XHA WT 3XHA WT 3XHA WT 3XHA

a cone a cone a cone a cone a cone a cone C I C S a i-1 b 1 b 1 b 1 g 1 g 1 g 1 RGS1 RGS1 RGS1 M1 M1 AGTRL1 AGTRL1 CCR10 CCR10 HTR6
a rod a rod a rod a rod a rod a rod a cone a cone a i-2 b 2 b 2 b 2 g 2 g 2 g 2 RGS2 RGS2 RGS2 M2 M2 AVPR1A AVPR1A CCRL1 CCRL1 HTR7A
a i-1 a i-1 a i-1 a i-1 a i-1 a i-1 a rod a rod a i-3 b 3 b 3 b 3 g 3 g 3 g 3 RGS3 RGS3 RGS3 M3 M3 AVPR2 AVPR2 CCRL2 CCRL2 HTR7B
a i-2 a i-2 a i-2 ai-2 a i-2 a i-2 a i-1 a i-1 a oA b 3S b 3S b 3S g 4 g 4 g 4 RGS3T RGS3T RGS3T M4 M4 BDKRB1 BDKRB1 CXCR1 CXCR1 HUMNPIIY20
a i-3 a i-3 a i-3 a i-3 a i-3 a i-3 a i-2 a i-2 a oB b 4 b 4 b 4 g 5 g 5 g 5 RGS4 RGS4 RGS4 M5 M5 BDKRB2 BDKRB2 CXCR2 CXCR2 LTB4R
a olf a olf a olf a olf a olf a i-3 a i-3 mb 4 mb 4 g 7 g 7 g 7 RGS5 RGS5 RGS5 ADCYAP1R1 CALCR CXCR3 CXCR3 LTB4R2
a sS a sS a sS a sS a sS a sS a oA a oA b 5 b 5 g 8 g 8 g 8 RGS7(S1) RGS7(S1) RGS7(S1) ADMR ADMR CALCRL CXCR4/NPY3R CXCR4/NPY3R MC1R MC1R
a sL a sL a sL a sL a sL a sL a oB a oB b 5L b 5L b 5L g 9 g 9 g 9 RGS7(S2) RGS7(S2) ADORA1 C3AR1 C3AR1 CXCR5 CXCR5 MC2R MC2R
a q a q a q a q a q a q C G g 10 g 10 g 10 RGS8 RGS8 RGS8 ADORA2A C5R1 C5R1 CXCR6 CXCR6 MC3R MC3R
a 11 a 11 a 11 a 11 a 11 a 11 a cone g 11 g 11 g 11 RGS9 RGS9 ADORA2B C5L2 C5L2 CX3CR1 CX3CR1 MC4R MC4R
a12 a12 a 12 a 12 a 12 a 12 a rod g 12 g 12 g 12 RGS9L RGS9L ADORA3 CCKAR XCR1/GPR5 XCR1 MC5R MC5R
a 13 a 13 a 13 a 13 a 13 a 13 a i1 Arf1 Arf1 Arl1 Arl1 g 13 g 13 g 13 GAIP GAIP GAIP ADRA1A CCKBR CMKLR1 MTNR1B
a 14 a 14 a 14 a 14 a 14 a 14 a i2 Arf3 Arl2 Arl2 RGS10 RGS10 RGS10 ADRA1B CCBP2 CCBP2 CMKLR2 NMU1R
a 15(16) a 15(16) a 15(16) a 15(16) a 15(16) a 15(16) a i3 Arf4 Arf4 Arl3 Arl3 RGS13 RGS13 RGS13 ADRA2A CCR1 CCR1 CNR1(L) CNR1(L) NMU2R
a z a z a z a z a z a z a oA Arf5 Arf5 Arl4 Arl4 RGS14S RGS14S RGS14S ADRA2B CCR2 CCR2 CNR2 CNR2 NPY1R NPY1R
a oA a oA a oA a oA a oA a oA a oB Arf6 Arf6 Arl5 Arl5 RGS16 RGS16 RGS16 ADRA2C CCR3 CCR3 CRHR1 NPY2R NPY2R
a oB a oB a oB a oB a oB a oB Arf Prot Arl7 Arl7 RGS17 RGS17 RGS17 ADRB1 CCR4 CCR4 CRHR2 NPY3R/CXCR4
RGSZ1 RGSZ1 RGSZ1 ADRB2 CCR5 CCR5 CRTH2 NPY4R NPY4R
ADRB3 CCR6 CCR6 CYSLTR1 CYSLTR1 NPY5R NPY5R
AGTR1 AGTR1 CCR8 CCR8 CYSLTR2 CYSLTR2 NTSR1 NTSR1
AGTR2 AGTR2 CCR9 CCR9 DRD1 NTSR2
DRD2 OPRK1
HA/WT Myc/WT GV HA/GV Myc/GV SN HA/SN Myc/SN DRD5 OPRL1
H-Ras H-Ras H-Ras H-Ras H-Ras H-Ras H-Ras H-Ras H-Ras H-Ras HA Myc Myc/PBRQ HA/PBRQ HA Myc Myc/PBRQ HA/PBRQ EDG1 EDG1 OPRM1
N-Ras N-Ras N-Ras N-Ras N-Ras N-Ras N-Ras Cdc42 Cdc42 Cdc42 Cdc42 Cdc42 EDG2 EDG2 OXTR
K-Ras K-Ras K-Ras K-Ras K-Ras K-Ras K-Ras K-Ras K-Ras K-Ras Cdc42p Cdc42p Cdc42p Cdc42p Cdc42p EDG3 EDG3 P2RY1
M-Ras M-Ras M-Ras M-Ras M-Ras M-Ras M-Ras RhoA RhoA RhoA RhoA RhoA RhoA RhoA RhoA RhoA RhoA RhoA EDG4 EDG4 P2RY2
R-Ras R-Ras R-Ras R-Ras R-Ras R-Ras R-Ras RhoB RhoB RhoB RhoB RhoB EDG5 EDG5 P2RY4 P2RY4
Rap1A Rap1A Rap1A Rap1A Rap1A Rap1A Rap1A Rap1A Rap1A Rap1A RhoC RhoC RhoC RhoC RhoC EDG6 EDG6 P2RY5
Rap1B Rap1B Rap1B Rap1B Rap1B Rap1B Rap1B Rap1B Rap1B Rap1B Rac1 Rac1 Rac1 Rac1 Rac1 Rac1 Rac1 Rac1 Rac1 EDG7 EDG7 P2RY6
Rap2A Rap2A Rac2 Rac2 Rac2 Rac2 Rac2 EDG8 EDG8 P2RY10
Rap2B Rap2B Rac3 Rac3 Rac3 Rac3 Rac3 EDNRA EDNRA P2RY11
RhoD RhoD EDNRB EDNRB P2RY12
RhoG RhoG EDNRBL PTAFR
WT HA RhoH RhoH F2R PTGER1
AGS1 AGS1 Ran Ran Rab1A Rab1A Rab6A Rab6A Rab14 Rho6 Rho6 HA Myc Myc/PBRQ HA/PBRQ F2RL1 PTGER2 PTGER2
Dex Ras2 RCC1 RCC1 Rab2A Rab2A Rab6B Rab6B Rab18 Rho7 Rho7 Cdc42 Cdc42 F2RL3 PTGER3 PTGER3
RalA RalA Rab3A Rab3A Rab6C Rab6C Rab23 Rab23 Rho8 Rho8 Cdc42p Cdc42p FKSG79 FKSG79 PTGER4
RalB Rab3B Rab3B Rab7 Rab7 Rab26 Rab26 TCL TCL RhoA RhoA RhoA RhoA RhoA FKSG80 PTGFR
Rheb2 Rheb2 Rab3D Rab3D Rab8 Rab8 Rab27A Rab27A TC10 TC10 RhoB RhoB FPR1 PTGIR PTGIR
Rig Rab4A Rab4A Rab9 Rab9 Rab27B RhoC RhoC FPRL1 PTHR1
Rin Rin Rab4B Rab4B Rab10 Rab10 Rab28 Rac1 Rac1 Rac1 Rac1 FPRL2 PTHR2
Rit Rit Rab5A Rab5A Rab11A Rab11A Rab30 Rac2 Rac2 FSHR TBXA2R TBXA2R
TC21 Rab5B Rab5B Rab11B Rab11B Rab31 Rac3 Rac3 FZD4 RE2
Rab5C Rab5C Rab13 Rab32 GALR1 SALPR SALPR
Rab33A genes cloned in the Creator donor vector, pDNR-1r GCGR SCTR
Rab35 genes cloned in the expression vector, pcDNA3.1+ GHSR(1a) SSTR1 SSTR1
WT 3XHA GHSR(1b) SSTR2 SSTR2
RAMP1 RAMP1 GLP2R SSTR3 SSTR3
RAMP2 RAMP2 GNRHR SSTR4 SSTR4
RAMP3 RAMP3 GPR35 GPR35 SSTR5 SSTR5
GRPR STRG/GPR88
ION-Ch HCRTR1 TACR1 TACR1
WT HRH1 TACR2
HTR3A HRH2 TACR3 TACR3
HRH4 TAR1 TAR1
HTR1A TAR3 TAR3
HTR1B HTR1B TAR4 TAR4
HTR1D TAR5 TAR5
HTR1E TM7SF1
HTR1F TRHR
HTR2A TSHR
HTR2B UTR2
HTR2C VIPR2
HTR4 HTR4
HTR5A
G p r o t e i n C o u p l e d R e c e p t o r s (G P C R)
PTX-R
RAS
WT WT WT WT
ARF/ARL
Rho
WT
Grb2
RhoGDI
GPCR activity
modifying proteins
WT TN (Dominant negative)
Rho
WT
a
b
g
Ran
RGS
g b
WT
a
WT
H E T E R O T R I M E R I C G P R O T E I N S
WT GV (Constitutively active)
WT
Adapters
GPR GoLoco
WT
rAGS3S
rAGS3L
Ras-like Rab
WT
LGN
Phenotypes: WT, Wild type (i.e., native protein); QL constitutively-active due to a glutatmine (Q) to leucine (L) mutation which eliminates
GTPase activity; GV constitutively-active due to a glycine (G) to valine (L) mutation which eliminates GTPase activity; GT, SN, TN dominant
negative phenotypes due to glycine (G) to threonine (T), serine (S) to asparagine (N) or threonine (T) to asparagine (N) mutations,
respectively; PTX-R, Pertussis toxin resistant due to mutation of a cysteine (C) to either isoleucine (I), glycine (G) or a serine (S), as indicated;
XTP, dominant negative phenotype due to double glutamine (Q) to leucine (L) and aspartate (D) to asparagine (N) mutations;
Tags: FLAG, Epitope-tagged with the FLAG epitope; EE, Epitope-tagged with an internal glutamate-glutamate epitope; HA, Epitope-tagged
with the hemagglutinin epitope in triplicate (3X); Myc, Epitope-tagged with the 10 amino-acid myc epitope in duplicate (2X).
RGS, regulator of G protein signaling; RhoGDI, Rho guanine nucleotide dissociation inhibitor; GPR, G protein regulatory; ION-Ch, Ligand-
gated ion channel.
GPCR, G protein coupled receptors: M, acetylcholine (muscarinic subtype); ADM, adrenomedullin; AD, adrenergic; ADO, adenosine; AGT,
Angiotensin; AVP, arginine-vasopressin; BDKR, bradykinin; CALCRL, calcitonin receptor-like; C3A, complement component 3A; C5,
complement component 5; CC, Chemokine; CCRL, chemokine (C-C motif) receptor-like; CCBP, chemokine binding protein; CXCR, chemokine
(C-X-C motif) receptor; CX3CR, chemokine (C-X3-C motif) receptor; XCR, chemokine (C motif) receptor; CN, cannabinoid, CCK,
cholecystokinin; CYSLT, cysteinyl leukotriene; D, dopamine; EDG, endothelial differentiation G-protein-coupled; EDN, endothelin; FKSG79,
putative purinergic receptor; FP, formyl-peptide; GHS, growth hormone secretagogue; HCRT, hypocretin/orexin; H, histamine; HT, 5-
hydroxytryptamine/serotonin; LTB4, leukotriene B4; MC, melanocortin; NPY, neuropeptide Y; OP, opioid; OXT, oxytosin; P2, purinergic;
PTAF, platelet-activating factor; PTGE, prostaglandin E; PTGF, prostaglandin F; PTGI, prostaglandin I2; SALP, somatostatin and angiotensin-
like peptide; SST, somatostatin; TAC, tachykinin; TA, trace amine.
UMR cDNA Resource Center Clone Collection, 4 MARCH, 2004 (749 constructs)
www.cdna.org