Method

Principle

Urea

Modified Berthelot's Reaction

Creatinine

Jaffe's Reaction

Triglycerides

Cholesterol

CHOD-PAP (Cholesterol Oxidase Peroxidase Aminophenazone)

Sample

Reagent

Urea

10 uL

1000 uL

Creatinine

200 uL

Triglycerides
Cholesterol

Cholesterol

Enzymatic colorimetric

Urease (Enzyme)

Phosphate buffer
pH 7.0 and pH <13

Sodium Nitroprusside
Hypochlorite and
Sodium salicylate

Photometric colorimetric

Picric acid (Reactant)

NaOH pH >7.0

Enzymatic colorimetric with

LCF (Lipid Clearing Factor)

Measured against, at __nm

Cholesterol Esterase
--> Cholesterol
Oxidase

Indophenol
(Green dye)

Peroxidase

Factor for Serum

Factor for Urine

80.8 g/L

1343 mmol/L

2000 uL

Against air (measured twice)

at 492nm (490-510nm)

2 mg/dL

176.8 umol/L

100 mg/dL

8840.2 umol/L

10 uL

1000 uL

Reagent Blank at 500 or

546nm

200 mg/dL

2.28 mmol/L

10 uL

1000 uL

Reagent Blank at 500 or

546nm

200 mg/dL

5.17 mmol/L

13 mg/dL

500 mg/dL

1150 umol/L

44200 umol/L

1000 mg/dL
11.4 mmol/L
750 mg/dL
19.3 mmol/L

333 - 583
1.7 - 8.3 mmol/L mmol/24 hrs.
1000-1500
mg/24 hours

Serum

@G&G

13.3 mmol/L

400 g/L

20-35 g/24 hrs.

220 mg/dL

80 mg/dL

6600 mmol/L

10-50 mg/dL

Peroxidase
Phosphate buffer
Quinoneimine (Light
4-Aminophenazone
and
pH 6.5
pink)
Phenol

Reagent Blank at 578nm

(570-600nm)

400 mg/dL

Urine

150 mg/dL

SI units

66.6 mmol/L

Serum/Plasma

Quinoneimine (Light
4-Aminoantipyrine and
orange)
4-Chlorophenol

Conventional

Urine

Reference Values

Creatinine-Picrate
Men: 0.6-1.1
complex (Orange- Women: 0.5- 0.9
red)
mg/dL

SI units

Serum

Triglycerides

Buffer

Conventional

Linearity

Creatinine

Product detected/
measured
Catalyst/Chromogens

Enzymes/Reactants

Lipase --> Glycerol

GPO-PAP (Glycerol Phosphate Oxidase - Enzymatic colorimetric with
PIPES buffer
Kinase --> Glycerol 3
Peroxidase Aminoantipyrine)
LCF (Lipid Clearing Factor)
pH 7.5
Phosphate Oxidase

Pipetting Scheme

Urea

Reagents

Pipetting for correction

Specimen of choice

Interferences

1:1 with distilled water

Serum, plasma except ammonium

heparinated plasma and urine

HgB >200 mg/dL; Bilirubin >10 mg/dL (not

influenced if low)

1:5 with saline (9%)

Serum, heparinized plasma and urine

Temperature must be kept at 25 C

1:4 with saline (9%)

Serum and heparinized or EDTA-plasma

Hgb >150 mg/dL; Bilirubin >40 mg/dL ;

ascorbate >4 mg/dL

1:2 with saline (9%)

Serum and heparinized or EDTA-plasma

Hgb >200 mg/dL; Bilirubin >5 mg/dL

(not influenced if low)

BSMT