PCR RT-PCR

1. Conventional PCR is more time Realtime

consuming as it uses gel PCR is less time consuming as it can
electrophoresis to analyze the detect amplifications
amplified during the early phases of the reaction
PCR products.
2. Traditional PCR collects Realtime
data at Endpoint PCR collects data at the exponential
of the reaction. growth phase of PCR
3. The end point results of the Results of the realtime
conventional PCR may not be very PCR are very precise.
precise
4. Conventional PCR is less sensitive than Realtime
Realtime PCR is more sensitive than conventional
PCR PCR.
5. Conventional PCR has very poor Realtime
resolution PCR can detect very little changes due
. to the high resolution
6. Conventional PCR cannot discriminate Realtime
between dead and live bacteria. PCR can discriminate between dead and
live bacteria.