Energy Metabolism

AMPK Signaling Pathway

Low Glucose,
Hypoxia, Ischemia,
Heat Shock
Brain AICAR
Leptin Insulin
Exercise Receptor
-Adrenergic Histamine Other RTKs
Adiponectin Receptor Thrombin
Glucose Receptor
Glut1 Insulin
Glut4

PLC Gq
[cAMP]
AMP + ADP
ATP
Glut1
vesicle PKA Ras
[Ca2+]
PI3K
Glut
Glut Translocation STRAD B-Raf
Trafficking CaMKK LKB1
MO25
Glut4
vesicle TXNIP Erk Akt
Acute Regulation of Me

Glycogen TBC1D1
GS p90RSK
Synthesis
Glycolysis PFKFB3 AMPK TSC2

d Autophagy
TSC1 Rheb
HMG-CoA
Sterol/Isoprenoid Reductase ACC Raptor
Synthesis
Malonyl mTOR GL
CoA
tabolism

ism an
CPT1
ULK1 4E-BP1
Fatty Acid p70

tabol
Oxidation HSL FIP200 ATG13 S6K
Autophagy
Me
tein
p300 Beclin
Pro

ATGL SREBP-1
PI3K Atg14
Lipolysis Class III
MYPT1
Tau MBS85 HNF4 SirT1
HDAC4 Fatty Acid
CLIP170 Synthase
HDAC5
Microtubules Lipogenesis
HDAC7 TORC2/
Myosin CRTC2
C Light
yt Chain2
os
ke Actin PGC1 m
let lis
al
Si Dynamics abo
gn CREB et
ali fM
ng CRY1 Gluconeogenesis o lo
FoxO ntr
al Co
tion
scrip
Tran

For pathway key and background please visit:

www.cellsignal.com/ampk
 Key Products for Energy Metabolism
Visit www.cellsignal.com/ampklist for full listing of antibodies, related kits, and reagents.

ANTIBODIES APPLICATIONS REACTIVITY Phospho-AMPK

(Thr172) (40H9)
kDa
100
#3676 Acetyl-CoA Carboxylase (C83B10) Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Hm Rabbit mAb 80

#11818 Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb WB, IP, IHC-P, IF-IC H, M, R #2535: WB analysis Phospho-
of extracts from 60 AMPK
#5831 AMPK (D5A2) Rabbit mAb WB, IP H, M, R, Mk, B (Thr172)
293 cells, untreated 50
#2532 AMPK Antibody WB, IP H, M, R, Hm, Mk (-) or treated with
#2793 AMPK (F6) Mouse mAb WB H, M, R, Hm, Mk Oligomycin #9996 40
(0.5 M, 30 min; +),
#2535 Phospho-AMPK (Thr172) (40H9) Rabbit mAb WB, IP, IHC-P H, M, R, Hm, Mk, Dm, Sc, (C, Z, B, Pg) 100
using #2535 (upper)
#2795 AMPK1 Antibody WB H, Mk, (Pg) or AMPK Antibody 80

#2757 AMPK2 Antibody WB, IP H, Mk #2532 (lower).

60 AMPK
#4185 Phospho-AMPK1 (Ser485)/AMPK2 (Ser491) Antibody WB H, M, R, Mk
50
#4150 AMPK1/2 (57C12) Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Hm, Mk
#4181 Phospho-AMPK1 (Ser108) Antibody WB, IP H, M, R, Mk 40
+ Oligomycin
#4458 ARK5 Antibody WB H, M, R, Mk, (B)
#8057 ASCT2 (D7C12) Rabbit mAb WB, IP, IF-IC, F H, M, Mk
#4332 ATP-Citrate Lyase Antibody WB H, M, R, Mk
#4331 Phospho-ATP-Citrate Lyase (Ser455) Antibody WB, IP H, M, (R)
#12980 Catalase (D4P7B)XP Rabbit mAb WB, IP, IF-IC H
#14309 Citrate Synthase (D7V8B) Rabbit mAb WB, IF-IC H, M, R, Hm, Mk
#4850 COX IV (3E11) Rabbit mAb WB, IP, IHC-P, IHC-F, IF-IC, F H, R, Mk, Z, B, Pg
#11967 COX IV (4D11-B3-E8) Mouse mAb WB, IP, IHC-P, IF-IC H, M, R, Mk
#12963 Cytochrome c (6H2.B4) Mouse mAb IP, IF-IC H, M, R
#12362 DLAT (4A4-B6-C10) Mouse mAb WB, IP, IF-IC H, Mk
#3838 EAAT2 Antibody WB, IF-F M, R, (H)
#8137 IDH1 (D2H1) Rabbit mAb WB, IP, IF-IC, F H, M, R, Mk COX IV (3E11) Rabbit mAb #4850: Confocal
IF analysis of HeLa cells using #4850 (green).
#12652 IDH2 (D7H6Q) Rabbit mAb WB, IP H, M, R, Mk
Actin filaments were labeled with Alexa Fluor
#3582 LDHA (C4B5) Rabbit mAb WB, IHC-P, IF-IC H, Mk 555 Phalloidin #8953 (red). Blue pseudocolor =
#8176 Phospho-LDHA (Tyr10) Antibody WB H, M, R DRAQ5 #4084 (fluorescent DNA dye).

#3047 LKB1 (D60C5) Rabbit mAb WB H, M, R, Mk

#13031 LKB1 (D60C5F10) Rabbit mAb (IHC Formulated) IHC-P H, M, Mk LKB1 (D60C5)

12
29
KI

S
kDa
Rabbit mAb

PC
CO
CA

L9
140
#3482 Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb WB H, M, R, Mk #3047: WB 100
#2716 MO25/CAB39 (C49D8) Rabbit mAb WB, IP H, M, R, Mk analysis of 80
extracts from
#2183 NFAT3 (23E6) Rabbit mAb WB H, M, R 60
LKB1
various cell lines 50
#12721 NRF2 (D1Z9C)XP Rabbit mAb WB, IP, F, ChIP H, M, Mk using #3047. 40
#3820 PDHK1 (C47H1) Rabbit mAb WB, IP H, M, R, Mk, (B)
#3205 Pyruvate Dehydrogenase (C54G1) Rabbit mAb WB, IHC-P H, M, R, Mk
#11998 SDHA (D6J9M) XP Rabbit mAb WB, IP, IHC-P, IF-IC H, M, R, Hm, Mk
#8071 Succinyl-CoA Synthetase (D8A11) Rabbit mAb WB, IP, IF-IC H, M, R, Mk
#13002 Syntaxin 1A Antibody WB H, M, R, (Mk)
#8076 TFAM (D5C8) Rabbit mAb WB, IP, IF-IC H
#14670 UCP1(D9D6X) Rabbit mAb WB, IP M, (H)
#13508 VAMP2 (D6O1A) Rabbit mAb WB, IP, IHC-P, IF-F, IF-IC H, M, R
#12331 VGLUT1 Antibody WB M, R, (H)
#12691 xCT/SLC7A11 (D2M7A) Rabbit mAb WB, IP H, (Mk)

RELATED KITS AND ASSAYS

#7959 PathScan Phospho-AMPK (Thr172) Sandwich ELISA Kit
#9839 AMPK Subunit Antibody Sampler Kit
#8660 PPAR Regulated Fatty Acid Metabolism Antibody Sampler Kit
#8674 Mitochondrial Marker Antibody Sampler Kit
#5132 PhosphoPlus LKB1 (Ser428) Antibody Duet

SDHA (D6J9M) XP Rabbit mAb #11998: IHC analysis of paraffin-embedded human colon carcinoma
15FLYENGY_REFM103ENG_00

using #11998 in the presence of control peptide (top) or antigen-specific peptide (bottom).

APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry
/ -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all
species expected / ( ) 100% sequence homology

2015 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, PathScan, PhosphoPlus, and XP are trademarks of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners.

For Research Use Only. Not For Use In Diagnostic Procedures.

www.cellsignal.com/ampk