OBSERVATION

Escherichia coli, is not inhibited by bile salts and crystal violet and is a gram-
negative bacterium. The pink color of the bacterial growth indicates E. coli is
able to ferment lactose.

Klebsiella pneumoniae ferments lactose and produces pink colonies on MAC.

Salmonella typhimurium, is not inhibited by bile salts and crystal violet and is a
gram-negative bacterium. The absence of color in the bacterial growth indicates
S. typhimurium is unable to ferment lactose.
OBSERVATION

Pseudomonas aeruginosa: Green colonies with typical matted surface and rough
periphery, non-fermentative.

Escherichia coli: Opaque yellow colonies with a slightly deeper yellow center,
indicating fermentative.

OBSERVATION
V. cholera and its biotype Eltor ferment sucrose, which results in a pH shift and
production of yellow-brown colonies.

V. parahaemolyticus is a sucrose non-fermenting organism and produces colonies

with blue to green centers.

OBSERVATION
Colonies of Salmonella typhi are typically with pinpoint black centres.

Salmonella spp. that produce H2S appear as blue-green colonies with black
centers. H2S producers form black-centered colonies in the presence of ferric
ammonium citrate and sodium thiosulfate.
Salmonella typically produce clear colonies with distinct black centres (hydrogen
sulphide: H2S +) on HE.

OBSERVATION
S. aures: Beta Hemolysis, complete lysis of Red Blood Cells, causing a clearing of
blood from the medium under and surrounding the colonies.

Streptococcus pneumonia: Alpha hemolysis, partial lysis of the RBC to produce a

greenish-gray or brownish discoloration around the colony.

Group A beta hemolytic streptococci-Streptococcus pyogenes

Beta Hemolysis, complete lysis of Red Blood Cells, causing a clearing of blood
from the medium under and surrounding the colonies.

OBSERVATION
S. aures: positive result with yellowing of red/pink medium, growth indicated.

S. epidermidis: pink colonies

OBSERVATION
Haemophilus influenzae: Non hemolytic, opaque cream-to-gray colonies
(accompanying Sheep blood agar shows no growth)

Neisseria gonorrhoeae: Colonies on Chocolate agar are pinkish-brown and

translucent, exhibit smooth consistency and defined margins, and are typically
0.5-1 mm in diameter.