Intro to Molecular Med Samplex

Modified Matching Type. Match the underlying principle with the different DNA tests
A. Replication
B. Reverse Transcription
C. Complementary pairing
D. A and B
E. None of the above

A 1. In vivo DNA cloning

D 2. cDNA cloning
A 3. In vitro DNA cloning
D 4. RT-PCR
C 5. RFLP
E 6. Western Blot
C 7. DNA microchips
A 8. DNA fingerprinting

Match the different DNA tests with the procedure involved:

A. PCR
B. Genomic DNA cloning
C. cDNA cloning
D. RFLP
E. RT-PCR

B 9. Restriction digestion, ligation, and transformation

C 10. Reverse transcription restriction digestion, ligation and transformation
A 11. DNA denaturation, Primer annealing and Primer extension
E 12. Reverse transcription, DNA denaturation, Primer annealing and Primer extension
D 13. Restriction digestion, gel electrophoresis, DNA transfer to a paper, Blotting with a
complementary probe, autoradiography

Match the different tests with the reagents/enzymes and molecules involved

A. PCR
B. RT-PCR
C. Genomic Cloning
D. DNA fingerprinting
E. RFLP
F. Sangers DNA sequencing
G. Maxam-Gilbert DNA sequencing

B 14. dNTPs, primer, taq polymerase, mRNA

A 15. dNTPs, primer, taq polymerase, DNA
C 16. Plasmid, restriction enzyme, ligase, DNA
D 17. Restriction endonuclease, DNA probe, VNTR
F 18. dNTPs, ddNTPs, primer, taq polymerase, DNA
C 19. Ampicillin, e. coli, restriction enzyme, ligase
G 20. dimethyl sulphate, piperidine, high salt, mild acid

Bonus 10 points
What are the 2 enzymes that can be used to combine two different DNAs?
DNA ligase
DNA polymerase