The document provides an overview of important concepts in mycology, including the basic structure of fungi consisting of hyphae and spores. It lists various culture media and their uses, such as Sabouraud dextrose agar as a general medium, Czapek's medium for culturing Aspergillus, and cornmeal agar for visualizing chlamydospores. It also outlines techniques for identifying different fungal species based on their microscopic morphology on various stains or growth characteristics on specific media.
The document provides an overview of important concepts in mycology, including the basic structure of fungi consisting of hyphae and spores. It lists various culture media and their uses, such as Sabouraud dextrose agar as a general medium, Czapek's medium for culturing Aspergillus, and cornmeal agar for visualizing chlamydospores. It also outlines techniques for identifying different fungal species based on their microscopic morphology on various stains or growth characteristics on specific media.
The document provides an overview of important concepts in mycology, including the basic structure of fungi consisting of hyphae and spores. It lists various culture media and their uses, such as Sabouraud dextrose agar as a general medium, Czapek's medium for culturing Aspergillus, and cornmeal agar for visualizing chlamydospores. It also outlines techniques for identifying different fungal species based on their microscopic morphology on various stains or growth characteristics on specific media.
General culture media SDA Czapeks medium Aspergillus Serum at 37OC germ tube Cornmeal agar Chlamydospore Media with olive oil Malassezia furfur Hair baiting test T. mentagrophytes Trichophyton skin, hair, nails Red color, tear drop conidia T. rubrum Favic chandelier (antler) T. schoenleinii Coenocytic Phycomycetes / Zygomycetes Ascospore Saccharomyces cerevisiae C. neoformans Niger birdseed (+), urease (+), phenol oxidase (+), India ink Arthrospore Geotrichum, Coccidioides, Trichosporon, Aureobasidium Blastospores yeast 10% KOH direct microscopic, clearing LPCB common fungal stain Wright / Giemsa H. capsulatum Dichotomous hyphae Aspergillus Thrush, diaper rash, Moniliasis Candida Actinomyces Sulfur granules Nocardia modified acid fast Macro- microconida Dermatophytes Sputum systemic mycoses CHO assimilation ID yeast, free of CHO BHIA- dimorphic fungi C. immitis high risk to lab personnel Chlorampenicol and cycloheximide inhibitory agent for mycosel / mycobiotic