Professional Documents
Culture Documents
Method:
HCV RNA is copied into complementary DNA by reverse transcriptase,
followed by amplification of the conserved 5' untranslated region by
real time PCR.
Comments:
1. The lower detection limit of the assay is 15 IU/ml.
2. Sensitivity of this assay is 99% and specificity 99%.
3. Almost 10-15% patients who develop anti HCV antibody spontaneously
clear HCV infection.
Cautions:
1. HCV RNA detection in serum may be intermittent due to fluctuation of
viral titer in blood. Therefore, a single negative HCV RNA test should
not be used to indicate that an individual is not infected.
2. Result of HCV RNA test carried out in different laboratories must
not be compared due to differences in sensitivity and specificity of
the methods used.
3. Correlation of results with clinical features and other laboratory
findings is highly recommended.
This is a computer generated report therefore does not require any signature. Date: 12/04/2016 17:38:17