Stadium Road, P.O.

Box 3500 ,
Karachi - 74800, Pakistan
Time Square Collection Unit Tel:(021) 34813398

Medical Record # : L24198772 (TS115886) 1

Patient Name : SAQIB,SHAHID Age / Gender : 30Y / Male
Specimen ID : 2021:VI81463R Location : TIME
Clinical Information / Comments: Requesting Physician : Self Referred
Account # : C33127782 - OSR
ACCEPTED AS PER PATIENT REQUEST Requested on : 20/03/2021 - 16:51
Collected on : 20/03/2021 - 16:51
Reported on : 21/03/2021 - 11:07
SOURCE : NASAL SWAB

SWAB + COVID19 (Package) [ Final Report ]

This is an electronic report & not

Qualitative PCR Result: Negative

2019 novel Coronavirus (SARS-CoV-2) is not Detected.

to be used for any legal purposes

Clinical Interpretation:

If the Virus RNA is Not Detected, it indicates that SARS-CoV-2 is absent in the
patient's specimen. However, as virus titers may vary in early infection, in
symptomatic individuals this may be influenced by the stage of the infection if
present.

Method:

This is a nucleic acid amplification-based test(NAAT)using a real-time

reverse-transcription (RT) polymerase chain reaction (PCR) method. Cobas® SARS-CoV-2
is based on fully automated sample preparation (nucleic acid extraction and
purification) followed by PCR amplification and detection. Automated data management
is performed by the cobas® 6800 system. Selective amplification of target nucleic
acid from the sample is achieved by the use of target-specific forward and reverse
primers for ORF1/a non-structural region that is unique to SARS-CoV-2. Additionally,
a conserved region in the structural protein envelope E-gene for pan-Sarbecovirus
will also detect SARS-CoV-2 virus.

Comments:

1. The viral titers may fluctuate therefore a single negative SARS-CoV-2 coronavirus
RT-PCR test should not be used to rule out infection.
2. Correlation of results with clinical, serological and other laboratory findings
is highly recommended.
3.Detection of SARS-CoV-2 RNA may be affected by sample collection methods, patient
factors (e.g., presence of symptoms), and/or stage of infection.
4. As per the manufacturer's recommendations, the lower limit of detection of
SARS-CoV-2 target is CID50/mL of 0.007.
5. As with any molecular test, mutations within the target regions of cobas®
SARS-CoV-2 could affect primer and/or probe binding resulting in failure to detect
the presence of virus.
6.The performance characteristics of this assay have been validated by the AKUH
Continued ... 2
Dr. Zahra Hasan Dr. Tariq Moatter Dr. Zeeshan Ansar Ahmed Dr. Kiran Iqbal Dr. Asghar Nasir
PhD( UK), MS, Mphil, PhD MBBS, FCPS(Hematology) MSc (Microbiology), PhD MSc, PhD (UK)
Professor Professor Assistant Professor Senior Instructor Assistant Professor

Dr. Najia Ghanchi

MSc, PhD
Assistant Professor
 Stadium Road, P.O. Box 3500 ,
Karachi - 74800, Pakistan
Time Square Collection Unit Tel:(021) 34813398

Medical Record # : L24198772 (TS115886) 1

Patient Name : SAQIB,SHAHID Age / Gender : 30Y / Male
Specimen ID : 2021:VI81463R Location : TIME
Clinical Information / Comments: Requesting Physician : Self Referred
Account # : C33127782 - OSR
ACCEPTED AS PER PATIENT REQUEST Requested on : 20/03/2021 - 16:51
Collected on : 20/03/2021 - 16:51
Reported on : 21/03/2021 - 11:07
SOURCE : NASAL SWAB
Clinical Laboratory.

This is an electronic report & not

Refence:
Wölfel R, et al. Virological assessment of hospitalized patients with COVID-2019.

to be used for any legal purposes

Nature. 2020 May;581.

Report format as per College of American Pathologists guidelines on 05-08-2020

This is a computer generated report therefore does not require any signature.
Dr. Zahra Hasan Dr. Tariq Moatter Dr. Zeeshan Ansar Ahmed Dr. Kiran Iqbal Dr. Asghar Nasir
PhD( UK), MS, Mphil, PhD MBBS, FCPS(Hematology) MSc (Microbiology), PhD MSc, PhD (UK)
Professor Professor Assistant Professor Senior Instructor Assistant Professor

Dr. Najia Ghanchi

MSc, PhD
Assistant Professor