3/8/2017 PourplateMethod:Principle,Procedure,Uses,and(Dis)Advantagesmicrobeonline
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PourplateMethod:Principle,Procedure,Uses,and
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Pourplatemethodisusuallythemethodofchoiceforcountingthenumberofcolonyformingbacteriapresentin Join1,005othersubscribers
[Link],fixedamountofinoculum(generally1ml)fromabroth/sampleisplacedinthe
EmailAddress
[Link](approx.15mL)isthenpouredintothePetri
[Link],theplateisinvertedand Subscribe
incubatedat37Cfor2448hours.
[Link]
generallyaresmallinsizeandmaybeconfluentthefewthatgrowontheagarsurfaceareofthesamesizeand
[Link](bothlargeandsmall)colonyiscarefullycounted(usingmagnifying
colonycounterifneeded).Eachcolonyrepresentsacolonyformingunit(CFU). TripleSugarIronAgar(TSI):
Principle,Procedureand
Interpretation
Thenumberofmicroorganismspresentintheparticulartestsampleisdeterminedusingtheformula:
july16,2013
CFU/mL=CFU*dilutionfactor*1/aliquot
Catalasetest:principle,uses,
procedureandresults
Foraccuratecounts,theoptimumcountshouldbewithintherangeof30300colonies/[Link]
october7,2013
countableplateaseriesofdilutionsshouldbeplated.
GramStaining:Principle,Procedure
andResults
february2,2015
BloodAgar:Composition,
Preparation,UsesandTypesof
Hemolysis
august22,2013
API20ETestSystem:Introduction,
ProcedureResultsandInterpretations
may6,2015
CATEGORIES
Bacteriology
AnaerobicBacteriology
BacteriologyNote
BiochemicaltestsinMicrobiology
laboratorydiagnosisofBacterialDisease
MCQBacteriology
Thepourplatemethodofcountingbacteriaismoreprecisethanthestreakplatemethod,but,ontheaverage,it StructureofBacterialCells
virulencefactorsofpathogenicbacteria
willgivealowercountasheatsensitivemicroorganismsmaydiewhentheycomecontactwithhot,moltenagar
DifferenceBetween
medium. EmergingInfectiousDiseases
Immunology
Uses: MCQimmunology
serology
Thepourplatetechniquecanbeusedtodeterminethenumberofmicrobes/[Link] LifeofBidur
Microbialgenetics
advantageofnotrequiringpreviouslypreparedplates,andisoftenusedtoassaybacterialcontaminationoffood
Microbiology
stuffs.
CultureMediausedinMicrobiology
FunnyMicrobiology
MaterialsandEquipments MCQMicrobiology
MCQMycology
[Link] MedalsandawardsinMicrobiology
[Link](PCA)orNutrientAgar MicrobiologyforBeginners
3.Hotwaterbath45C recentdiscoveryinmicrobiology
[Link] StainingtechniquesinMicrobiology
[Link] Uncategorized
[Link] USMLEMicrobiologypracticequestions
7.Sterilecapped16*150mmtesttubes MolecularBiology
[Link](e.g.01,1.0and2.0mL) Mycology
labdiagnosisoffungaldisease
ProcedureofPourplatetechnique Parasitology
labdiagnosisofparasiticDisease
[Link] 1/4
3/8/2017 PourplateMethod:Principle,Procedure,Uses,and(Dis)Advantagesmicrobeonline
labdiagnosisofparasiticDisease
1.Preparethedilutionofthetestsampleexpectedtocontainbetween30300CFU/mL.(Followserialdilutiontechnique) MCQParasitology
[Link](0.1or1.0mL)ofdilutedspecimen ParasitologyNote
Principlesofsterilizationanddisinfection
SexuallyTransmittedInfections
Note:forthedetaildescriptionregardinguseofpipette,inoculationofsample,dilutiontechniqueetc,followthe
Virology
reference1.
LabdiagnosisofViralDisease
MCQVirology
Pouringthemoltenagarandincubation StructureofVirus
[Link](containing15mLofmeltedPlateCountAgaroranyotherstandardculturemedia)
fromthewaterbath(45C).
[Link]
withthelittlefingerofthelefthand.
[Link].
[Link]
handandpourthesterilemoltenagarintothe
Petridishandreplacethethelid.
[Link].
[Link]
mediumthoroughlyandtoensurethatthemedium
[Link]
theedgeofthepetridish.
[Link],
itwilltakeapproximately10minutes. Pouringthemoltenagarmedium
[Link]
37Cfor2448hours.
OverviewofPourplatemethodandspreadplatemethod
Results:
After2448hours,countallthecolonies(again:notethattheembeddedcolonieswillbemuchsmallerthanthose
whichhappentoformonthesurface).Amagnifyingcolonycountercanaidincountingsmallembeddedcolonies.
CalculateCFU/mLusingtheformula:CFU/mL=CFU*dilutionfactor*1/aliquot
(thevolumeofdilutedspecimen(aliquot)iseither0.1or1.0mL)
DisadvantagesofPourplatemethod
[Link]/andorspreadplatetechnique.
[Link].
[Link],onemustbecarefultoscore
thesesothatnoneareoverlooked.
[Link].
Referencesandfurtherreadings
[Link](SGM)
[Link] 2/4
3/8/2017 PourplateMethod:Principle,Procedure,Uses,and(Dis)Advantagesmicrobeonline
[Link](SGM)
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Hello,[Link],Iamworkingasa
lecturerandMicrobiologistatDepartmentofMicrobiologyandImmunology,PatanAcademyofHealth
Sciences,[Link]/difficult,leaveinthe
commentsbelow.
CapsuleStain:Principle,ProcedureandResults PathogenicMicrobeswithcharacteristicssmell(good
andbad)
3thoughtsonPourplateMethod:Principle,Procedure,Uses,and(Dis)
Advantages
Egwuatu REPLY
October16,2016at11:57amEdit
quitegood
michael REPLY
October26,2016at8:45pmEdit
Wouldanaerobicbacteriagrowinapourplateasinoculatedandincubatedabove?reason?
Prachi REPLY
February14,2017at11:03amEdit
Whyuse250mlsampleforwateranalysisthroughmembranefiltermethod
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