Name: Nivedita Prabhu Date: 9/11/17 Period: 4

Egg Osmosis Lab

Background:
Whenever two different solutions are separated by a selectively permeable membrane, an
osmotic system is established. Each cell, therefore, represents an osmotic unit.
Osmosis may be defined as diffusion of water molecules through a selectively permeable
membrane.

Purpose: To observe the effects of osmosis on an animal membrane.

Problem: How do various liquids affect the concentration of water inside and outside of an
egg?
Materials:
1 egg 200 mL vinegar
200 mL Karo syrup 200 mL water
1 beaker (400 mL) 1 plastic cup (capable of holding 200 mL)

Hypothesis:
Write in an if, then sentence.
If a substance with a higher solute concentration than the interior of the egg enters
the egg, then water will leave the egg's membrane and another substance with a lower
solute concentration than the egg's interior will enter the egg's membrane.
Procedure;
Day 1;
1. Label the side of your cup with the initials of everyone in your group and the number
of your class period with small letters.
2. Carefully mass your egg. Record the results in the data table.
3. Measure 200 mL of vinegar and pour into your cup. Record this amount under Volume
added in the data table. Nothing is recorded in the Volume remaining column for the first
day. 4. Place your egg into the cup. Record observations in the data table.

Day 4:
 5. Observe your egg and record in data table.
6. Carefully remove your egg from the vinegar, rinse gently with water then mass it.
Record mass.
7. Observe your egg and record in data table
8. Measure the amount of vinegar remaining in your cup. Record in the data table under
Volume remaining.
9. Measure 200 mL of syrup and place into your cup. Record volume added. Carefully place
your egg into the syrup.
Day 5:
10. Observe your egg. Record observations. Carefully remove your egg from the syrup, rinse
with water and mass. Record.
11. Measure the amount of syrup remaining in your cup use your fingers to wipe the
syrup out the cup into the beaker. Record. Pour the syrup down the large sink in the back.
Run warm water while pouring to ensure the sink doesnt stop up. Clean your table with
warm soap and water. Use warm soap and water to clean the beaker you used to measure
the syrup.
12. Measure 200 mL of water and place into your cup.
Day 6:
13. Observe your egg. Record observations. Carefully remove your egg and mass. Record.
14. Measure the amount of water remaining in your cup. Record.
Day Date Mass (g) Volume Volume Observations
Added (ml) remaining (ml)

1 9/8 66. 2 200 N/A When we observed the egg today, the
vinegar started to fizz as soon as we
added the egg inside the beaker.
4 9/11 98. 5 200 150 When we viewed t he egg t oday, the egg
was at the top floating in vinegar.
5 9/12 58. 9 200 225 When we studied the egg today, the
egg appeared deflated while sitting in
the syrup.
 6 9/13 101. 1 200 150 When we examined the egg today, the
egg's shell got deteriorated. The egg
was bigger and heavier while sitting in
the water.

 Answer the following on a separate sheet of paper.
Conclusions:
1. Vinegar is made of acetic acid and water. When the egg was placed in vinegar, which way did
the water molecules move? How do you know this? The water molecules moved in an outwards
direction from the egg. This action is called Osmosis. Osmosis is the diffusion of water
molecules through a selectively permeable membrane.

2. After the egg was placed in syrup, which way did the water molecules move in or out of
the egg? Using the correct terminology, describe what condition exists for the egg. The water
molecules moved in an inwards direction towards the egg. The correct terminology is Diffusion.
Diffusion - the movement of the particles from an area of higher concentration to a area of
lower concentration. The egg's shell deteriorated due to the movement of the particles.

3. How can you explain the amount of liquid remaining when the egg was removed from the
syrup? Since the egg's membrane is selectively permeable, which only allowed a certain amount
of the syrup to be present in the egg's membrane, just enough to shrink the egg.

4. When the egg was placed in the water after being removed from the syrup, which way did
the water move? Was your hypothesis validated? Explain why or why not. Graph your data.
Remember to label and scale correctly. Based on the data taken and the results of the
experiment, my hypothesis is correct. The acetic acid in vinegar removed the shell from the egg;
some water from the vinegar did move into the egg causing its initial mass increase. The syrup
solution was hypertonic, so water moved out of the egg in an outwards direction. When the
egg got transferred from the syrup to the distilled water, the higher solute concentration of
the water caused the water to move into the egg.