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and purification
For every application, sample and RNA type
Isolate and purify
RNA with confidence
Go ahead and push the limits of your research. Well be there
to support you with robust RNA kits, trusted RNA tools, and
experienced technical support, all backed by nearly 30 years
of leadership and innovation in RNA technologies.
Transcriptome purification 10
mRNA purification 11
RNA quantitation 20
Ordering information 22
RNA isolation
technology guide
Purify RNA from virtually any cell type with our broad range TRIzol
of RNA extraction methodologies. High RNA yield, purity, Plus RNA
Purification Kit
and integrity help ensure high-quality, accurate results in all Cat. No. 12183555
downstream research applications.
Low-to-medium sample
throughput (150 samples)
PureLink
Pro 96 Total RNA
Purification Kit
Cat. No. 12173011A
PureLink MagMAX mirVana
RNA Mini Kit Total RNA Isolation Kit
Cat. No. 12183018A Cat. No. A27828
Medium-to-high sample
throughput (>24 samples)
Low-to-medium sample
throughput (150 samples) Most tissues
MagMAX
FFPE Total
Nucleic Acid
Isolation Kit Medium-to-high throughput
Cat. No. 4463365
Small RNA
RecoverAll isolation
Total Nucleic Low-to-medium throughput
Acid Isolation (<16 samples) mirVana
Kit for FFPE miRNA
Cat. No. AM1975 Isolation Kit
Cat. No. AM1560 mirVana
PARIS Kit
Cat. No. AM1556
MagMAX mirVana
Total RNA Isolation Kit
Cat. No. A27828
4
Cells-to-C T Cells-to-CT TaqMan Power
1-Step 1-Step Power Gene Expression SYBR Green
TaqMan Kit SYBR Green Kit Cells-to-CT Kit Cells-to-CT Kit
Cat. No. A25605 Cat. No. A25601 Cat. No. 4399002 Cat. No. 4402953
PureLink
Pro 96 Total RNA
Purification Kit
Cat. No. 12173011A
MagMAX mirVana PureLink
Total RNA Single Pro 96 Total RNA
Isolation Kit 1-step 2-step PureLink Purification Kit
Cell-to-CT
Cat. No. A27828 qRT-PCR qRT-PCR qRT-PCR Kit
RNA Mini Kit Cat. No. 12173011A
Cat. No. 12183018A
Cat. No. 4458237 MagMAX mirVana
Total RNA Isolation Kit
Cat. No. A27828
Medium-to-high sample
throughput (>24 samples) TRIzol Plus RNA
Low-to-medium sample
Purification Kit Single cell
Cat. No. 12183555 throughput (150 samples)
Tissue Cells
Start here
(choose sample type)
Small volume
FFPE Blood (500 L)
Leukocytes
only
LeukoLOCK
Total RNA
Isolation System
RiboPure PureLink Stabilized Cat. No. AM1923
RNA Purification Total RNA Tempus tubes Blood-to-CT
Kit, Blood Blood Kit qPCR Kit
Cat. No. AM1928 Cat. No. K156001 Cat. No. 4449082
MagMAX
for Stabilized Tempus
Blood Tubes Spin RNA
RNA Isolation Kits Isolation Kit
Cat. No. 4451893 Cat. No. 4380204
Cat. No. 4451894
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Automated RNA purification
Highly versatile, adaptable, and flexible system
Optimize and automate your RNA purification workflow with Applied Biosystems MagMAX kits and Thermo
Scientific KingFisher magnetic particle processors.* A revolutionary particle separation technology
permanent magnetic rods and disposable tip combs with independent movement controlforms the basis
of both KingFisher Flex and KingFisher Duo Prime instruments. When used with compatible bead-based
reagents, such as MagMAX kits, these processors enable versatile automation of DNA and RNA extraction,
isolation, and purification procedures.
*KingFisher products not available in all regionsplease check with your sales representative for availability.
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Compact automated purification with
the KingFisher Duo Prime Magnetic
Particle Processor
This small footprint system is ideal for space-restricted
laboratories. Using a carousel of 2 plate positions, the
KingFisher Duo Prime Magnetic Particle Processor is
designed to process washing, incubation, and bead transfer
steps across partial plates of samples from a variety
of sources.
When MagMAX kits are used on the KingFisher Flex processor, labs will have a simple, fast system that is
designed to provide pure RNA ready for downstream applications such as real-time PCR and next-generation
sequencing. Benefits include:
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Total RNA purification
Quality, intact RNAsimple, high-yield workflows
5
Total RNA from rat liver, spleen, and brain was purified
4 Supplier B kit
using the PureLink RNA Mini Kit and supplier B kit
according to manufacturers instructions. In each 3
0
Liver Spleen Brain
Tissue type
8
Which product is right for your research?
TRIzol reagents PureLink kits MagMAX kits Cells-to-CT kits
Process a large amount Fast isolation of RNA from a High-throughput Process cells for
of tissue variety of samples purification of RNA gene expression
and DNA
Prep time 60 min <20 min 45 min 10 min
Sample types Most samples, particularly Bacteria, liquid, blood, cells, yeast, Cells, blood, plants Cultured cells
more difficult to lyse plants, tissue
Starting material 100 mg of tissue or 108 cells, 200 mg of tissue, 250 mg of 100 mg of tissue or 1100,000 cells
107 cells plant, 0.2 mL of blood, 5 x 108 yeast, 5 x106 cells
109 bacteria
Yield 1 106 epithelial cells: Up to 350 g Variable depending NA
815 g, tobacco leaf: 73 g on sample
High-throughput
compatible
Technology Organic extraction Silica membrane spin column/ Magnetic beads Crude lysate
filter plate
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Transcriptome purification
Comprehensive, truly representative
spectrum of RNA transcripts
Isolating high-quality RNA is a crucial first step in successful whole transcriptome analysis. Transcriptome
enrichment and concentration prepare the sample for numerous downstream applications, including RNA-Seq,
microarray analysis, library construction, and qRT-PCR.
Invitrogen RiboMinus Transcriptome Isolation Kits enrich RNA transcripts by depleting large ribosomal RNA
molecules from the total RNA sample. This unique technology offers:
Total
TotalRNA
RNA 18S
18S 28S
28S Total
TotalRNA
RNA
28S
28S
A RiboMinus
RiboMinusRNA
Total
TotalRNA
RNA
RNA
B 18S
18S 28S
28S
RiboMinus
RiboMinusRNA
Total
TotalRNA
RNA
RNA
RiboMinus
RiboMinusRNA
RNA
28S
28S RiboMinus
RiboMinusRNA
RNA
18S
18S
Fluorescence
Fluorescence
Fluorescence
Fluorescence
18S
18S
Fluorescence
Fluorescence
Fluorescence
Fluorescence
Migration
Migrationtime
time Migration
Migrationtime
time
Migration
Migrationtime
time Migration
Migrationtime
time
C Total
TotalRNA
RNA D Total
TotalRNA
RNA
RiboMinus
RiboMinusRNA
RNA 23S
23S 25/26S
25/26S RiboMinus
RiboMinusRNA
RNA
Total
TotalRNA
RNA Total
TotalRNA
RNA
RiboMinus
RiboMinusRNA
RNA 23S
23S 25/26S
25/26S RiboMinus
RiboMinusRNA
RNA
Fluorescence
Fluorescence
Fluorescence
Fluorescence
Fluorescence
Fluorescence
16S
16S
Fluorescence
Fluorescence
16S
16S 16S
16S
16S
16S
Migration
Migrationtime
time Migration
Migrationtime
time
Migration
Migration time
time Migration
Migrationtime
time
Figure 1. Invitrogen RiboMinus technology demonstrating greater than 95% rRNA
depletion. A. Human total RNA was isolated from HeLa cells. B. Mouse total RNA isolated from
mouse liver. C. Bacteria total RNA and isolated from E. coli. D. Yeast total RNA isolated from S.
cerevisiae. Samples were analyzed using the Agilent 2100 Bioanalyzer instrument.
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mRNA purification
Rapid, high-yield isolation of pure mRNA
Only 15% of the total RNA in a typical mammalian cell is poly(A) RNA or mRNA. We offer highly
efficient technologies to purify mRNA directly from your starting sample or from isolated total RNA.
The table below will help you choose the technology that is right for you.
Enrichment from
total RNA
Sample type Total RNA Liquid, RNA, FFPE and fixed, Liquid, RNA, FFPE and Total RNA, blood, mammalian
yeast, blood, tissue, viral, fixed, yeast, blood, tissue, tissue, and cells
plant, cells viral, plant, cells
Starting material 75 g of total RNA 2 x 107 cells, 1 x 104 cells, 100 to 1 x 106 cells, 4 mg of
2200 mg of tissue, 5 mg of tissue or plant tissue, 40 L of whole blood,
4400 mg of plant 100 ng100 g of total RNA
High-throughput
compatible
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MicroRNA and
small RNA purification
Rapid, quantitative recovery of small RNA Serum Donor 1
MagMAX mirVana mirVana PARIS
34
MicroRNA and other small RNAs have a substantial impact on biological processes including gene expression.
Average Ct values
However, traditional RNA isolation methods do not sufficiently recover these smaller RNAs. Our RNA experts
30
have developed RNA isolation kits that provide rapid, quantitative recovery of small RNAs from a variety of
sample types in as little as 10 minutes. The table below will help you choose the right product to purify small
RNAs for your specific application.
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The high-throughput capable Applied Biosystems MagMAXLet7c
miR16
mirVana miR221
Total RNA miR21
Isolation Kit miR26a
Average Ct values
30 30
26
22 22
Let7c miR16 miR221 miR21 miR26a Let7c miR16 miR221 miR21 miR26a
Figure 2. RNA was isolated from the serum of two donors and the levels of five microRNAs were measured by qRT-PCR. The MagMAX mirVana
Total RNA Isolation Kit, a new magnetic bead-based technology, was benchmarked against the gold-standard Invitrogen mirVana PARIS RNA Kit.
Serum Donor 2
MagMAX mirVana mirVana PARIS
34
Average Ct values
30
Sample types Bacteria, cells, yeast, plants, Plasma/serum, whole blood, Cells
tissue, viral tissue, cells, urine
Starting material 250 mg of tissue or 107 cells 100 mg of tissue or 5 x106 cells 10100,000 cells
High-throughput compatible
Technology Organic extraction and silica Magnetic beads (phenol-free) Crude lysate
membrane spin column
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13
Viral RNA purification
Rapid, efficient purification from cell-free biological fluids
30
25
Ct
20
15
10
5
0
2 x 102 2 x 103 2 x 104 2 x 105 2 x 106
Figure 3. Serum samples (200 L) were spiked with adenovirus DNA at the indicated PFU and
then DNA extraction was performed following the manufacturers recommended procedure
for the PureLink Viral DNA/RNA Mini Kit or supplier B kit.
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Fresh or
Environmental
frozen,
and
cell-free
biofluid
biological
samples
fluids
High-quality,
Low viral
pure
concentration
isolation
PureLink MagMAX
Viral RNA/ Viral RNA
DNA Kits Isolation Kit
High- High-
throughput throughput
compatible compatible
View all our tools for viral RNA and DNA capture and Select the right viral RNA isolation
purification, as well as qRT-PCR and qPCR analysis at kit for your research at
thermofisher.com/viral
thermofisher.com/viralRNA
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Real-time RT-PCR
without RNA purification
Exceptional speed and accuracy in
measuring gene expression
Invitrogen Cells-to-CT kits allow you to skip RNA purification by going straight from cultured cells, blood or
single cell samples to measuring relative gene expression by real-time RT-PCR (qRT-PCR). Cells-to-CT kits are
available in both Applied Biosystems TaqMan and SYBR Green formats, and offer:
96 TaqMan Assays : 16,500 Jurkat Cells
45
Extraordinary ease and speed96 samples can be processed for qRT-PCR in typically <10 min
40
Cells-to-CT Average CTs
Superior
30
performancedesigned for consistent accuracy, reproducibility, and sensitivity, with 10100,000 cells
Validated
25 kit workflowscomplete sets of reagents preoptimized to work efficiently right out of the box
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y = 0.9862x + 0.5252
R = 0.96308
15
35
31
23 y = -3.5328x + 37.427
20
y = -3.669x + 36.879 R = 0.99864
y = 0.9465x + 1.5865
21 R = 0.99972
R = 0.97682
15
19
10 17
10 15 20 25 30 35 40 0 1 2 3 4 5 6
Purified RNA average Ct values log(10) Number of HeLa cells per lysis
Figure 4. Data from 72 assays compares performance of the Cells-to- Figure 5. HeLa cells (10 to 100,000) were processed in triplicate using
CT 1-Step TaqMan Kit with a traditional approach using purified RNA. the Cells-to-CT 1-Step TaqMan Kit or RNA purified with an RNA spin
Lysate from 16,500 Jurkat cells was prepared for qRT-PCR on the Applied column method. Each set of samples was analyzed by qRT-PCR for
Biosystems 7900HT Fast Real-Time PCR System. beta-actin (ACTB) and XenoRNA targets using the Invitrogen Cells-to-CT
Control Kit on the 7900HT system.
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Cells-to-CT technology guide
TaqMan Gene
Expression
Cells-to-CT Kit
Cat. No. 4399002
TaqMan MicroRNA Single Cell-to-CT
Cells-to-CT Kit qRT-PCR Kit
Cat. No. 4391848 Cat. No. 4458237
cDNA archiving
Cells-to-CT
TaqMan detection 1-Step
miRNA &
chemistry TaqMan Kit
IncRNA
expression Cat. No. A25605
Gene
expression
from stabilized
Cells-to-CT
blood SYBR detection 1-Step Power
chemistry SYBR Green Kit
Cat. No. A25601
cDNA archiving
Stabilized Blood-to-CT
Nucleic Acid Preparation
Power
Kit for qPCR
SYBR Green
Cat. No. 4449082 Cells-to-CT Kit
Cat. No. 4402953
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RNA lab essentials
For avoiding, detecting and inhibiting RNase
RNA can be difficult to work with as it is readily degraded by RNases that are found in a variety of
environmental sources, such as bacteria and fungi, as well as flaked skin and hair. RNases are extremely
robust enzymes that can retain functionality at room temperature, and even after freeze/thaw cycles and
autoclaving. Consequently RNases are unaffected by many methods of decontamination, and strong
chemical methods are often required to eliminate them from surfaces and solutions.
However some basic precautions, including the use of RNase-free plastics and reagents, maintaining a clean
work surface and properly stabilizing tissue samples prior to storage will go a long way toward minimizing
experimental inconsistencies and failures. Invitrogen RNA essentials are a staple in labs that handle RNA,
giving researchers worldwide confidence in their results.
Even spleen tissue, an organ known for its high endogenous RNase content,
can be stored without jeopardizing the quality or quantity of RNA subsequently
isolated when the sample is stabilized with RNAlater.
n y n y n y
ve
r l ee d ne ve
r l ee d ne ve
r l ee d ne
Li Sp Ki Li Sp Ki Li Sp Ki
p53
GAPDH
p53
GAPDH
Figure 6. Northern blot of RNA from tissue treated with Invitrogen RNAlater Stabilization Solution. The
dissected tissue was submerged in approximately 5 volumes of RNAlater solution at room temperature. Samples were
stored at 37C for one day, room temperature (25C) for one week, or 4C for one month. A northern blot (of gel in the
top panel) that was hybridized with probes to p53 and GAPDH, demonstrates the integrity of RNA isolated from tissues
treated with RNAlater solution and stored at different temperatures (bottom panel).
18
Nuclease-free tips and tubes
Pipette tips and tubes are an easily overlooked source of RNase contamination.
We offer a range of RNase-free plastic pipette tips, PCR tubes, microcentrifuge tubes and
conical tubes. Each lot of Invitrogen tips and tubes undergoes rigorous testing and is
certified to be nuclease-free. View products at thermofisher.com/nucleasefreeplastics
Nuclease-free water
Preparing reagents and resuspending precipitated RNA with the appropriate grade water
is a crucial and often ignored first step for consistent experimental results. Even purified
water can have a high pH and minerals that can interfere with certain types of reactions.
Surface decontamination
Its safe to assume that most laboratory surfaces are contaminated with RNases, since
theyre exposed to the bacteria, fungi, flaked skin, and hair present in the environment.
Unfortunately, even trace quantities of RNases can lead to lower yields from in vitro
transcription reactions, degradation during RNA purification protocols and variable results
with qRT-PCR.
Sample stabilization
In order to isolate high-quality RNA, the tissue has to be either processed immediately after
harvest, snap-frozen or stabilized in an intermediary solution to preserve RNA integrity and
allow for storage.
19
RNA quantitation
Sensitive, accurate, affordable
Invitrogen Qubit fluorometric quantitation comprises the easy-to-use
Qubit 3 Fluorometer and sensitive Qubit quantitation assays. The assays
utilize fluorescent dyes that are selective to the target of interest. These
fluorescent dyes emit only when bound to the target molecules even at
low concentrations. Unlike UV absorbance, which measures anything
absorbing at 260 nm, including DNA, RNA, protein, or free nucleotides,
the Qubit assays avoid overestimation, making it ideal for precious
samples and challenging applications.
12 1.8
1.5
1.3
10
1.0
0.8
8 0.5
0.3
6 0.0
0.1 0.25 0.5 1.0
0
0.1 0.25 0.5 1 2 4 6 8 10
Figure 7. Accuracy and precision of the Qubit quantitation platform. Ten replicates of E. coli
ribosomal RNA at concentrations from 0.1 to 10 ng/L were assayed using an Invitrogen Qubit RNA
assay on the Qubit 3 Fluorometer. The same concentrations of DNA were measured in ten replicates
using a microvolume spectrophotometer. The concentrations indicated are the concentrations of
DNA in the starting samples, before dilution in the Qubit assay tubes.
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21
Ordering information
Product Quantity Cat. No. Product Quantity Cat. No.
MagMAX mirVana Total RNA Isolation Kit 96 preps A27828 50 preps 4458237
Single Cell-to-CT qRT-PCR Kit
400 preps 4458236
Transcriptome purification kits
Cells-to-CT Stop Solution 1 mL 4402960
RiboMinus Transcriptome
6 preps K155003 Cells-to-CT Bulk Lysis Reagents 2,500 preps 4391851C
Isolation Kit, yeast
RiboMinus Transcriptome Stabilized Blood-to-CT Nucleic Acid
12 preps K155004 Preparation Kit for qPCR, compatible
Isolation Kit, bacteria 50 preps 4449079
with either PAXgene or Tempus Blood
RiboMinus Eukaryote System v2 12 preps A15026 RNA Tubes
RiboMinus Plant Kit for RNA-Seq 8 preps A1083808 Stabilized Blood-to-CT Nucleic Acid
mRNA purification kits Preparation Kit for qPCR, compatible 200 preps 4449082
with PAXgene Blood RNA Tubes
Dynabeads mRNA Purification Kit 2 mL 61006
Stabilized Blood-to-CT Nucleic Acid
5 mL 61011 Preparation Kit for qPCR, compatible 200 preps 4449080
Dynabeads mRNA DIRECT Purification Kit
10 mL 61012 with Tempus Blood RNA Tubes
Dynabeads mRNA DIRECT Micro SYBR Green Cells-to-CT Control Kit 100 preps 4402959
2 mL 61021
Purification Kit
TaqMan Cells-to-CT Control Kit 100 preps 4386995
96 preps K1570-02
mRNA Catcher PLUS Purification Kit
960 preps K1570-03
microRNA and small RNA purification kits
mirVana miRNA Isolation Kit, with phenol 40 preps AM1560
MagMAX mirVana Total RNA Isolation Kit 96 preps A27828
TaqMan MicroRNA Cells-to-CT Kit 100 preps 4391848
Viral RNA purification kits
PureLink Viral RNA/DNA Mini Kit 50 preps 12280050
PureLink Pro 96 Viral RNA/DNA
4 plates 12280096A
Purification Kit
MagMAX-96 AI/ND Viral RNA Isolation Kit 384 preps AM1835
96 preps AM1836
MagMAX-96 Viral RNA Isolation Kit
5 x 96 preps AM1836-5
MagMAX Viral RNA Isolation Kit 50 preps AM1939
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Product Quantity Cat. No. Product Quantity Cat. No.
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To find out more, go to thermofisher.com/rnapreps