Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report

Pre-lab Quiz Results

You have not completed the Pre-lab Quiz.

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Experiment Results
Predict Question:
Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane?
Your answer : c. No, not at all.

Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average
molecular weight of a single amino acid is 135 g/mole.

Which of the following will be able to diffuse through the 200 MWCO membrane?
Your answer : c. glucose only

Stop & Think Questions:

The reason sodium chloride didn't diffuse left to right is that
You correctly answered: c. the membrane pore size was too small.

Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino
acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because
You correctly answered: d. glucose and albumin are both too large to pass.

The rate of diffusion for urea

You correctly answered: b. is slower than that for sodium because urea is a larger molecule.

Experiment Data:

Solute MWCO Solute Concentration Average Diffusion Rate

Na+ Cl- 20 9.00 0.0000
Urea 20 9.00 0.0000
Na+ Cl- 50 9.00 0.0150
Na+ Cl- 50 18.00 0.0300
Na+ Cl- 100 9.00 0.0150
Urea 100 9.00 0.0094
Glucose 200 9.00 0.0040
Albumin 200 9.00 0.0000

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Post-lab Quiz Results
You scored 75% by answering 3 out of 4 questions correctly.

1. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to
You correctly answered: b. increase the rate of diffusion.

2. Describe the difference between the rate of diffusion seen for sodium and urea.
You correctly answered: c. Urea diffused more slowly because it is larger than sodium.

3. Which of the following solutes did not pass through any of the membranes?
Your answer: a. glucose
Correct answer: b. albumin

4. When diffusion stops, we say the solution has reached

You correctly answered: a. equilibrium.

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Review Sheet Results
1. Describe two variables that affect the rate of diffusion.
Your answer:
1.- el tamao del soluto y de los poros de la membrana podrian afectar pues como sucede con el ClNa a una 20 MWCO el
tamao de la soluto no puede pasar por la menbrana
2.- la velocidad del trasporte pues la urea por ser una molecula mas grande se difende lentamente que el sodio

2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare
with your prediction?
Your answer:
-Porque la urea es una molecula demaciado grande con peso molecular de 60.07 y el poro es de 20 MWCO enotoces eso
inpidio que la urea pase al otro lado de la membrana.Mi resultado fue correcto pues en la simulacion me indica q la urea no
realiza difusion con el dato que me proporcina que la tasa promedia de difusion es 0

3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the
results compare with your prediction?
Your answer:
En la simulacion donde la membrana es de 200 MWCO el soluto de glucosa de 9 mM se difinde a un tiempo de 37min
donde la tasa promedio de difusion es 0.004(mM /min )
pero en la albumina de 9mM con la misma menbrana no se realiza difusion
Los resultados fueron correctos con mi preccion pues glucosa si realiza la difision pero la protina por se una molucila
demaciado grande en si paso moecular no puede pasar por el poro de la membrana

4. Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea.
Your answer:
cloruro de sodio
urea
glucosa
albumina

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