Professional Documents
Culture Documents
1. Zurien van der Merwe of Laerskool Hennopspark (Gauteng) won 4. Anel Jansen van Vuuren and Faheema Ebrahamof Selly Park
gold for her project on the one-day fly and its role as bio-indica- Convent in Rustenburg did research into the best container for
tor in water quality. keeping a cup of coffee hot.
2. Louis van Biljon of Laerskool Menlopark has a clever idea for pre- 5. Amanthi Weerasingh of the Diocesan School for Girls
venting nasty bee stings when one enjoys a cold drink from a can. (Namibia) won gold for her research into aluminium.
3. Mahadi 6. Jean
Makwanazi and Basson and
Litsesana Mopeli Jaco de
of Sentinel Primary Jager of
(Free State) Suiderhof
entered a project Primary
on the magic School
touch of garlic. (Namibia)
They received a built a
gold medal for working
their clever idea. solar oven.
Extract DNA fact file
By: Professor Valery Corfield
DNA ● DNA stands for
from deoxyribonucleic acid.
This experiment will wheatgerm ● Itfromis abuilding
chemical substance made
blocks that form
allow you to extract long, thin strings.
Separate the DNA solution
● The DNA strings, called
one of the building blocks from the mixture
molecules, are packed very
of life – isolated DNA – from This step is easy. Just let the mixture tightly into the nucleus of cells.
stand and allow the solids to settle ● The DNA molecules twist around
plant cells. Although each DNA out. Then gently pour the liquid into each other and form a spiral
molecule is too small to see, if another glass, until it is about a quar- ladder – the DNA double helix.
ter full. Take care that the solids do not
you follow the instructions, you mix with the solution. The solution in
● DNA double helixes are
organised into 23 pairs of
will end up with visible DNA. the new glass now contains the DNA chromosomes in every cell in
You will need: in a dissolved form. your body.
● A cup of wheatgerm (from health shops or some Extract the dissolved DNA ● This set of chromosomes is the
grocery stores) from the solution instruction manual to make YOU.
● Table salt Take the quarter-filled glass, fill it up ● Each different instruction is called
● Clear alcohol (cane spirit, gin or rubbing alcohol with alcohol and stir very gently. As a gene.
from the chemist) you stir, you will notice that the DNA ● The gene instructions are written
● Green dishwashing liquid (not the gel type) precipitates out as very fine white in a DNA code – the genetic
● Lemon juice (fresh or bottled) threads. You can leave this mixture code.
to further allow the DNA to settle. ● New coded copies are made
● Two glass bottles or large glasses
Gently pour the liquid off and ... when the DNA double helix
● A sieve or strainer
there you have DNA! unzips down the middle.
● Clean water
Break down the cell walls
of the wheatgerm
In a large glass, dissolve one level tablespoon of salt
in 300 ml of tap water. Add four squirts of lemon
juice. Now add half a cup of wheatgerm to the solu-
tion and stir gently for 15 minutes. The lemon juice will
break down the cell walls of the wheatgerm. Press
this mixture through the sieve and discard the liquid.
You will be left with a soggy pulp. Do the same for the
other half a cup of wheatgerm. The pulp you now
have contains the cell contents without the cell walls.
Dissolve the DNA
Put one level tablespoon of salt in 300 ml of water,
stir the mixture until the salt is dissolved and add
six teaspoons of alcohol. Add nine large drops of
the washing-up liquid and stir gently. Add the
soggy pulp from step one and stir it gently for
about 20 minutes. During this period, the deter-
gent in the washing-up liquid will dissolve the DNA
into the mixture. Now add about 10 level tea-
spoons of salt and stir gently for 10 minutes.
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