6X Protein Loading Buffer

For 50ml: 30% glycerol 15ml

Stacking Buffer 28ml
6mM EDTA 600l of 0.5M
10% SDS 5g
60mM DTT 0.4626g
Bromphenol Blue 6mg

Bromphenol Blue take Sodium Salt to avoid pH-ing.

10X SDS Running Buffer

For 1liter : 30.2g Tris Base (MW 121.14)
10g SDS (MW 288.38)
144g Glycine (MW 75.07)

Coomassie stain

45% Methanol
7.5% Acetic acid
0.1% Brilliant Blue

DestainI Destain II

30% Methanol 5% Methanol

10% Acetic acid 7% Acetic acid
5% glycerol
 6X DNA Loading Buffer

Front runs at~30bp

60mM EDTA pH8 6ml of 0.5M
0.3% SDS 300mg
60% glycerol 35ml
Orange G( Sigma #O3756) pinch

OR:
70% glycerol
Orange G

50X TAE Buffer

For 1L : 242g Tris Base (MW 121.14)

57.1 ml glacial acetic acid
32.2g Na2EDTAx2H2O (MW 372.24)