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AFelip Sterilization ParisOct2008 PDF
AFelip Sterilization ParisOct2008 PDF
Alicia Felip
OVERVIEW
Thorgaard, 1983
Scientific knowledge Purdom et al., 1983
Industrial application Ihssen et al., 1990
Practical knowledge gaps
Main reserach axes
Benfey, 1999
Hulata, 2001; Felip et al., 2001
EUROPEAN FARMED FISH Piferrer et al., 2007
SHOCK Treatments
1N Pressure Chemical
2nd polar body shocks shocks
Sperm
(1N) (1N)
Thermal
shocks
3N
Variables
Timing
Intensity
Duration
Triploids (3n)
Atlantic salmon
(Salmo salar)
Type of 3n Survival Animal Growth
shock (%) (%) stage performance
Pressure 100 88.6 Juveniles 2n = 3n
Heat 100 92.5 Adults 3n > 2n
Commercial-scale : Canada
Rainbow trout
(Oncorhynchus mykiss)
Type of 3n Survival Animal Growth
shock (%) (%) stage performance
Heat 100 57.7 Juveniles 2n = 3n
Adults 3n > 2n
Commercial-scale : Canada, France, Japan
Androgens
Neomales XX X XX
Phenotipically males Normal fish
Triploidization
100% females XX
genotipically/phenotipically
and sterile
The future prospects for aquaculture breeding in Europe
Paris, October 2008
Scientific knowledge
Sea bass
(Dicentrarchus labrax)
Type of 3n Survival Animal Growth
shock (%) (%) stage performance
Pressure 100 71 Juveniles 2n = 3n
Cold 89-100 40-80 Adults 2n > 3n
Inicial tests for commercial-scale : France
Sea bream
(Sparus aurata)
Type of 3n Survival Animal Growth
shock (%) (%) stage performance
Heat 100 40 Juveniles 2n = 3n
Questionable interest??
Atlantic cod
(Gadus morhua)
-------
Meagre
(Argyrosomus regium)
Turbot
(Scopthalmus maximus)
Type of 3n Survival Animal Growth
shock (%) (%) stage performance
Cold 90-100 60-80 Juveniles 2n = 3n
Adults 3n > 2n
Inicial tests for commercial-scale : Spain
Halibut
(H.hipoglossus)
Type of 3n Survival Animal Growth
shock (%) (%) stage performance
Cold 92-95 50-82 Juveniles 2n = 3n
Heat 84 20 Adults 2n > 3n
Inicial tests for commercial-scale : France
Sole
(Solea solea)
Grass carp
(Ctenopharyngodon idella)
Type of 3n Survival Animal Growth
shock (%) (%) stage performance
Cold 50-100 20 Juveniles 2n = 3n
Heat 87 50 2n > 3n
Pressure 100 70
Comercial-scale: USA
Nile tilapia
(Oreochromis niloticus) Type of 3n Survival Animal Growth
shock (%) (%) stage performance
Cold 83.3-100 variable Juveniles 2n = 3n
Heat - - 3n > 2n (separated)
Males > Females
Pressure - -
---------
The future prospects for aquaculture breeding in Europe
Polyploidy in aquaculture
GAMETE MANIPULATION
9 Evaluation of broodstock
SHOCK TREATMENT
5 min DUR
TRIPLOIDY PROCEDURE
1200
1000 A D ip lo id s
Weight ( g )
800 T rip lo id s
600
400
P < 0.05
200
40
B
Length ( cm )
30
20 P > 0.05
10
1.4
1.3
1.2
1.1
0 5 10 15 20 25 30 35 40 45 50 55 60
A g e ( m o n th s )
10 Juveniles
(8) Ictalurus punctatus Wolters et al. (1982)
8 (7) +
Oreochromis niloticus + Bramick et al. (1995)
N = 16
6 Heteropneustes fossilis Tiwary et al. (1997)
+
4 Oreochromis aureus + Valenti (1976)
Silurus glanis + Krasznai & Marian (1986)
2 (1) Oncorhynchus mykiss Solar et al. (1984)
-
0 Lepomis gibbosus - Kerby et al. (1995)
Oncorhynchus kisutch - Withler et al. (1995)
10 Monosex stocks
(All-females) Salmo salar - Galbreath et al. (1995)
8 Misgurnus mizolepis NC Kim et al. (1994)
Salmo salar NC McGeachy et al. (1995)
6 N = 3 Oreochromis niloticus NC Hussain et al. (1995)
4 (3) Oreochromis aureus NC Chang et al. (1993)
2 (+) positive, (-) negative, (NC) no change
Basant et al. (2004)
0
3n > 2n 3n = 2n 3n < 2n
Felip (2000)
TRIPLOIDY EFFECTS 9 Evaluate triploid performance in terms of reproduction
Public concern