Plasmodium falciparum PLACENTAL MALARIA VACCINE CANDIDATE VAR2CSA

I. INTRODUCTION: Abstract + Introduction (In general, not too detailed)

II. MATERIALS AND METHODS

1. Upstream processing
a. Plasmodium falciparum culture (List or diagram)
b. Protein production (Pictures)
Vector construction
Transformation and Expression
2. Downstream processing
a. Extraction and Purification +
b. Testing Animal immuniizations
Antigenicity of DBL-domains in three different animal species (ELISA)
Affinity purified FV2 specific antibodies
IgG reactivity against native VAR2CSA protein expressed on the surface of infected
erythrocytes (IE) (ELISA, flow-cytometry)
The functional capacity of the induced antibodies was measured (inhibition of CSA-
binding assay)
Anti-FV2 IgG reactivity against individual DBL-domains by testing the levels of antibodies
against individual DBL-domains in serum from animals immunized with FV2. (ELISA)
Differential recognition of DBL4 in ELISA-peptide array analysis
c. Structure modelling
d. Statistical analyses

III. DISCUSSION: Too much =[[[[[[[[

REFERENCES

Pinto VV, Ditlev SB, Jensen KE, et al. Differential induction of functional IgG using the Plasmodium
falciparum placental malaria vaccine candidate VAR2CSA. PLoS ONE. 2011;6(3):e17942.

http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0017942#s4