Quiz on Restriction Enzymes

1. Sticky ends are DNA fragments cleaved by a restriction enzyme so that one strand is
longer than the others.
2. Restriction enzymes cleave a certain specific sequence of bases.
3. In nature, the purpose of restriction enzymes is to protect the bacterium from virus attack
by cutting up foreign DNA.
4. Cutting DNA with a particular restriction enzyme produces restriction fragments that can
be separated by gel electrophoresis.
5. DNA ligases bind nucleotides together.
6. A gel electrophoresis is often followed by a Southern blot because the gel is difficult to
handle; the Southern blot allows for analysis by technicians.
7. All restriction enzyme recognition sites are read the same forward and backward.
8. Restriction map is a diagram of DNA showing the cut sites of a series of restriction
enzymes.