10/26/2017 Peptide solubility and stablity

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 (0) My Cart Peptide Solubility Guidelines
Peptide Chemical Formula and Gene Synthesis 
MW Calculator (https://www.genscript.com/account/customerpeptide.html)
(https://www.genscript.com/tools/peptide-
Proper peptide solubilization is one of the most important factors in a successful peptide
molecular-weight-calculator)
assay. Improper peptide solubilization results in inaccurate peptide concentration
Peptide Property Calculator calculations, which can introduce experimental error into data or result in experimental
(https://www.genscript.com/tools/peptide-
property-calculator) failure. However, finding the ideal solvent in which to dissolve your peptide is often a
challenge. The steps outlined below guide you through the process for determining the
Antigen Prediction Tool
(/antigen_design.html) best solvent for maximum solubility of your custom peptide. It is best to test your peptide's solubility by first using a
minute amount of peptide. Distilled, sterile water should be tried as a solvent first, especially when the peptide has less
Amino Acid Code
than five residues. For other peptides, optimum conditions for peptide solubility are based on the peptide sequence.
(/Amino_Acid_Code.html)
Amino Acid Property Chart
(/amino_acid_structure.html)
How to dissolve your peptide
Peptide Library Design Tools
(/peptide_screening_tools.html) 1. Calculate the length of the peptide. Generally, for those less than 6 amino acids, peptide can dissolve in pure water.
Frequently Asked Questions For those more than 6 amino acids, the dissolve principle is according to its overall charge of the peptide and the
(/faq_for_peptide.html) degree of hydrophobic.
Recommended Peptide Purity 2. Assign a value of -1 to acidic residues which include Asp (D), Glu (E), and the C-terminal -COOH. Assign a value of
Guidelines
(/recommended_peptide_purity.html) +1 to basic residues which include Arg (R), Lys (K), His (H), and the N-terminal -NH2. Calculate the overall charge of
the entire peptide.
Peptide Solubility Guidelines
(/peptide_solubility_and_stablity.html) 3. (Option 1-1) If the overall charge of the peptide is negative, try to dissolve the peptide in water first. (Option 1-2)If
Peptide Handbook (/peptide- water fails, add NH4OH (< 50 μl) and then dilute the peptide solution to the desired concentration. If the peptide
handbook.html) contains Cys, do not use NH4OH to dissolve it. Try the alternative method listed below. (Option 1-3) If the peptide still
Peptide Storage and Handling does not dissolve, add DMSO (50-100μl) to solubilize the peptide and then dilute the peptide solution to the desired
Guidelines concentration.
(/peptide_storage_and_handling.html)
4. (Option 2-1)If the overall charge of the peptide is positive, try dissolving the peptide in water first. (Option 2-2)If water
Peptide Antigen Design
fails, try dissolving the peptide in a 10%-30% acetic acid solution. (Option 2-3) If the peptide still does not dissolve, try
Guidelines
(/peptide_design_guideline.html) dissolving the peptide in a small amount of DMSO, and then dilute the peptide solution to the desired concentration.

Why Peptide Assays Fail 5. Peptides having an overall charge of zero usually dissolve best in an organic solvent. First, try adding acetonitrile,
(/peptide_assay_failure.html) methanol, etc. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute
Peptide Applications the solution with water to the desired concentration. Peptides containing Cysteine (C), Methionine (M) or Tryptophan
(/applications_of_custom_peptides_synthesis.html)
(W) are sensitive to oxidation by DMSO. We advise that peptides dissolved in DMSO be used immediately or stored
Case Studies at -20°C (or preferably −80°C) prior to use.
(/peptide_cases_studies.html)
Peptide Publications The above recommendations are schematically represented below:
(/peptide_synthesis_publications.html)
Peptide News Click on the bubbles that correspond to the overall charge of your peptide for guidance on how to dissolve your peptide.
(/peptide_news.html) or Request a solubility test when you request a quote (https://www.genscript.com/account/customerpeptide.html) for
Promotions your custom peptide (by typing 'solubility test' in the comments box) and let us make peptide solubilization easy for you.
(/peptide_promotion.html)
Peptide Webinars (/peptide-
webinars.html) DISSOLVING INSTRUCTIONS

Flowchart for Dissolving Peptides:

GenScript Custom Peptides

Calculate the change peptides

(/peptide.html?src=leftbar) <0(Acidic peptide) >0(Basic peptide)

0 (Neutral peptide)

Add water Add water

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10/26/2017 Peptide solubility and stablity

Add organic solvents

(acetonitrile,methanol,isopropanol,etc.)
Add 10%~30% acetic acid
Add NH4OH (<50μl)
a. Cys-containing
peptides
(/contact.html) b. Very hydrophobic
peptides Add TFA(<50μl)
c. Tend-to-aggregate
peptides

a b c

Add small Add small Add 8M urea or

amount amount 6M
DMF DMSO guanidine*HCL

Dilute to the desired concentration

Peptide Solution

Peptide Stock Solution 1

Peptide Storage 2

Note:

1. It is recommended that the concentration of the stock solution be around 1-2 mg of peptide per mL of solution. This is
dilute enough so that relatively small volumes (< 100 μL ) of peptide can be used in an assay, minimizing the effect of
the solvents initially used for solubilization.

2. The stability of each peptide depends on its sequence information. We suggest storing lyophilized peptides at -20 °C.
Once in solution we recommend that you aliquot your peptide into tubes and store at -20 °C(or preferably −80°C). It is
recommended that peptides containing methionine, cysteine, or tryptophan residues be stored in an oxygen-free
atmosphere to avoid oxidation.

Quotations and Ordering

For quotations, please use our Secure Instant Online Quotation/Order system
(https://www.genscript.com/account/customerpeptide.html). However, you may also contact us by email
(mailto:%70%65%70%74%69%64%65%40%67%65%6e%73%63%72%69%70%74%2e%63%6f%6d), phone (1-732-
885-9188), fax (1-732-210-0262), or via our Secure Messaging System
(https://www.genscript.com/online_request/general?src=peptide).

Ordering Standard Peptide Synthesis

(https://www.genscript.com/account/customerpeptide.html)

Please use our online ordering system and either a PO (Purchase Order) or credit card to receive GenScript's fastest
service. For batch order, please download and complete Standard Peptide Batch Order Form
(/gsfiles/techfiles/Standard_Peptide_Batch_Order_Form.xls) and email it to peptide@genscript.com
(mailto:%70%65%70%74%69%64%65%40%67%65%6e%73%63%72%69%70%74%2e%63%6f%6d). Our customer
service representatives are available 24 hours, Monday through Friday to assist you.
We accept POs and major credit cards ( ). A 7% New Jersey sales tax will be applied to orders shipped
to New Jersey. Your credit card will be billed under "GenScript" Click here to download our credit reference form
(/gsfiles/techfiles/CreditReferenceForm_GenScript.doc). For international orders, we must apply the full charge at the
time the order is placed. In the unlikely event that any given order cannot be filled, our guarantee will take the form of a
full refund.

Order by email: peptide@genscript.com (mailto:peptide@genscript.com)

Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188

Fax: 1-732-210-0262 1-732-885-5878

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10/26/2017 Peptide solubility and stablity

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