Date: 27.02.

2017
Microbial Validation conducted at Metro Cash & Carry JAIPUR – DC 45 For checking the
effectiveness of cleaning and sanitation at different places in meat area Swab test has taken with the help of “3M
Clean Trace” equipment. 3M Clean Trace Luminator was used to check the cleaning efficacy of chemicals by taking
Swabs before and after cleaning.

Readings before chemical Reading after chemical

SL NO Chemical used Area/ equipment
treatment (RLU) treatment (RLU)

1 Satol AC - 0.5% Epoxy floor 13190 97

crates 8050 90
Satol AC - 0.5% & Satol PA
2 cutting board 25007 45
-0.25%
Cutting knifes 23786 47

Hand swab
32
3 Mr. Ayub 1230
Hand wash & Hand
sanitizer (meat associate)

Concentration of Chemicals Used for Cleaning and sanitation

Chemical Concentration Contact Time

Satol AC 0.5% 10 Minutes
Satonil PA 0.25% 1 Minute # ATP
Handwash and Hand Sanitizer Ready to Use 30 -40 Seconds
Adenosine Triphosphate is present in all living organisms and the same is Universal Unit of energy used in all living Organisms.
# High Contamination = large amount of ATP = More light produced in reaction =High RLU reading on Luminator and vice versa.

Metro Cash & Carry DC 45 Representative : Mr Amreesh Sharma

Satol Chemical’s Representative: Mr. Kunjan Kumar