Hubungan Polimorfisme Gen CYP2E1 / RSA I dengan Kejadian Kanker Kolorcktal di

Sumatera Selatan

Deny Safitri, Sarup Singh, M. Alscn Arlan, Efman EU Manawan, Irsan Saleh
Departemen Bedah Rumah Sakit dr. Mohammad Hoesin Palembang/ Fakultas Kedokteran
Universitas Sriwijaya Palembang

Abstrak
Latar Belakang: Kanker kolorektal (KKR) merupakan tumor ganas yang ditemukan pada
kolon dan rektum. Faktor yang mempengaruhi terjadinya kanker kolorektal diantaranya pola
makan yaitu makanan yang mengandung nitrosamin. Gen CYP2E1 merupakan gen penyandi
protein CYP2E1 yang berperan dalam metabolisme nitrosamin. Aktivasi nitrosamin sebagai
substrat CYP2E1 mengarah kepada berkembangnya penyakit kanker termasuk kanker
kolorektal. Belum ada data mengenai polimorfisme gen CYP2E1/Rsa1 dan hubungannya
dengan kanker kolorektal.
Tujuan: Mengetahui hubungan polimorfisme gen CYP2E1 / Rsa 1 dengan kejadian kanker
kolorektal di Sumatera Selatan.
Metode: Penelitian observasional analitik dengan desain kasus kontrol. Analisa gen CYP2E1
dengan tehnik polymerase chain reaction-restriction fragment length polymorphism (PCR-
RLFP) menggunakan enzim restriksi endonuklease Rsa1
Hasil: Distribusi genotip c1c1, c1c2, dan c2c2 pada penderita KKR 72,5% : 22,5% dan 5%,
pada kontrol 77,5% : 22,5% dan 0%. Distribusi alel c1 dan c2 pada penderita KKR 83,75% :
16,25%, pada kontrol 88,75% : 11,25%, dengan menggunakan uji chi square tidak didapatkan
hubungan yang bermakna antara polimorflsme gen CYP2E1 dengan kejadian KKR di
Sumatera Selatan ditinjau dari genotipe, OR=1,30; 95%CI=0,70-1,83; p=0,796, maupun alel
OR=1,53 : 95%CI=0,61-3,81:p=0,491.
Simpulan: Polimorfisme gen CYP2E1 / Rsa 1 tidak berhubungan dengan kejadian kanker
kolorektal di Sumatera Selatan.
Kata kunci: CYP2E1; polimorfisme; genotip; alel; PCR-RLFP; kanker kolorektal.
 Association between Polymorphism of CYP2E1 / RSA I Gene with Colorectal Cancer
Patients in South Sumatra

Deny Safitri, Sarup Singh, M. Alscn Arlan, Efman EU Manawan, Irsan Saleh
Department of Surgery dr. Mohammad Hoesin Palembang / Faculty of Medicine Sriwijaya
University Palembang

Abstract
Background: Colorectal cancer (KKR) is a malignant tumor found in the colon and rectum.
Factors that affect the occurrence of colorectal cancer include diet that is food containing
nitrosamine. The CYP2E1 gene is a CYP2E1 protein-encoding gene that plays a role in
nitrosamine metabolism. Activation of nitrosamines as substrate CYP2E1 leads to the
development of cancer, including colorectal cancer. There is no data on CYP2E1 / Rsa1 gene
polymorphism and its association with colorectal cancer.
Objective: To determine the relationship of CYP2E1 / Rsa 1 gene polymorphism with the
incidence of colorectal cancer in South Sumatra.
Methods: An observational analytic study with case control design. CYP2E1 gene analysis
with polymerase chain reaction-restriction fragment length polymorphism (PCR-RLFP)
technique using restriction endonuclease Rsa1.
Results: Genotype distribution of c1c1, c1c2, and c2c2 in patients with KKR 72.5%: 22.5%
and 5%, in control of 77.5%: 22.5% and 0%. The distribution of alleles c1 and c2 in the KKR
patients was 83.75%: 16.25%, at control 88.75%: 11.25%. Using chi square test, there is no
significant association was found between CYP2E1 gene polymorphisms and KKR events in
South Sumatra evaluated from genotype, OR = 1,30; 95% CI = 0.70-1,83; p = 0.796, or allele
OR = 1.53: 95% CI = 0.61-3.81: p = 0.491.
Conclusion: CYP2E1 / Rsa 1 gene polymorphisms are not associated with the incidence of
colorectal cancer in South Sumatra.
Keywords: CYP2E1; polymorphism; genotype; alleles; PCR-RLFP; colorectal cancer.