11/21/2017 The Effect of Hydrogen Gas on a Mouse Bilateral Common Carotid Artery Occlusion | SpringerLink

The Effect of Hydrogen Gas on a Mouse Bilateral Common

Carotid Artery Occlusion
Brain Edema XV pp 61-63 | Cite as

Kimihiro Nagatani (1) Email author (naval.kimi@gmail.com)

Satoru Takeuchi (1)
Hiroaki Kobayashi (1)
Naoki Otani (1)
Kojiro Wada (1)
Masanori Fujita (2)
Hiroshi Nawashiro (1)
Shoichi Tachibana (2)
Katsuji Shima (1)

1. Department of Neurosurgery, National Defense Medical College, Tokorozawa, Japan

2. Division of Environmental Medicine, Research Institute, National Defense Medical College, Tokorozawa, Japan

Conference paper
First Online: 08 March 2013

1 Citations
1 Mentions
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1.9k Downloads

Part of the Acta Neurochirurgica Supplement book series (NEUROCHIRURGICA, volume 118)

Abstract

https://link.springer.com/chapter/10.1007/978-3-7091-1434-6_10 1/5
11/21/2017 The Effect of Hydrogen Gas on a Mouse Bilateral Common Carotid Artery Occlusion | SpringerLink

In recent studies, molecular hydrogen selectively reduced the levels of hydroxyl radicals in vitro and exerted a therapeutic
anti-oxidant activity in a rat middle cerebral artery occlusion model. The aim of this study was to investigate the effect of
hydrogen gas on a mouse bilateral common carotid artery occlusion (BCCAO) model. Male C57BL/6J mice were subjected to
transient BCCAO with a nontraumatic aneurysm clip. The mice were divided into three groups: sham, BCCAO, and BCCAO
treated with 1.3 % hydrogen gas. Cerebral blood flow (CBF) in the cortex was measured sequentially for both hemispheres
with a non-invasive and noncontact laser Doppler blood perfusion imager during the procedure. Vital signs were also
recorded. Oxidative stress evaluated by measuring the level of 8-hydroxy-2’-deoxyguanosine (8-OHdG), neuronal injury in
the hippocampal CA1 sector, and brain water content were assessed 24 h after ischemia. The hydrogen gas treatment had no
significant effect on vital signs or CBF values. However, the reduction of the expression of 8-OHdG, the decrease in the
neuronal injury in the hippocampal CA1 sector, and the attenuation in brain water content were observed in hydrogen-
treated mice. In conclusion, hydrogen gas might be effective in a mouse BCCAO model.

Keywords

Hydrogen Bilateral common carotid artery occlusion Mouse Ischemia/reperfusion injury Brain edema

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About this paper

Cite this paper as:
Nagatani K. et al. (2013) The Effect of Hydrogen Gas on a Mouse Bilateral Common Carotid Artery Occlusion. In: Katayama Y., Maeda T., Kuroiwa T. (eds)
Brain Edema XV. Acta Neurochirurgica Supplement, vol 118. Springer, Vienna

DOI (Digital Object Identifier) https://doi.org/10.1007/978-3-7091-1434-6_10

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