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2 Guide For IDD Managers Eng PDF
2 Guide For IDD Managers Eng PDF
Assessment of
iodine deficiency disorders and
monitoring their elimination
This document is intended primarily for managers of national program-
mes for the prevention and control of micronutrient malnutrition.
It sets out principles governing the use of surveillance indicators in imple-
menting interventions to prevent, control, and monitor iodine deficiency
disorders (IDD). It presents methods for monitoring iodine status and
determining urinary iodine and provides guidelines on the procedures for
monitoring salt iodine content, whether at the factory, importation site, or
household level. It also gives guidance on conducting surveys to assess
iodine status in populations.
Finally, indicators are presented for monitoring progress towards achie-
ving the goal of sustainable elimination of IDD as a significant public
health problem.
Abbreviations vi
Preface vii
1 Introduction 1
1.1 About this manual 1
1.2 Defi nitions 3
1.3 Monitoring and evaluating IDD control programmes 3
1.4 Indicators described in this manual 4
2 IDD and their control, and global progress in their elimination 6
2.1 The iodine deficiency disorders 6
2.2 Correction of iodine deficiency 8
2.3 Universal salt iodization (USI) 10
2.4 Iodine supplementation 12
2.5 Sustainability 12
2.6 Global progress in the elimination of IDD 14
2.7 Challenges for the future: consolidating the achievement 14
3 Indicators of the salt iodization process 17
3.1 Factors that determine salt iodine content 17
3.2 Determining salt iodine levels 20
3.3 Indicators for monitoring at different levels 22
4 Indicators of impact 28
4.1 Overview 28
4.2 Urinary iodine 28
4.3 Thyroid size 34
4.4 Blood constituents 38
5 Monitoring and evaluation methods 44
5.1 Overview 44
5.2 Monitoring and evaluation of salt iodization programs 44
5.3 Iodine status assessment 47
5.4 Thyroid function assessment 48
5.5 Common monitoring methods 48
5.6 Combined micronutrient deficiency surveys 51
iii
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
iv
CONTENTS
List of figures
Figure 1 National IDD control programming cycle 9
Figure 2 A monitoring and evaluation system for salt iodization 23
Figure 3 Anatomic description of the thyroid gland 62
Figure 4 Transverse scan 64
Figure 5 Longitudinal scan 64
Figure 6 Frequency table and histogram to show distribution of
urinary iodine values after iodization in Cameroon 82
Figure 7 Flow chart for Network Country Review 85
v
Abbreviations
vi
Preface
Knowledge obtained over the last decade through research and practical
use of the Assessment of Iodine Deficiency Disorders and Monitoring their
Elimination guidelines, edited by WHO in collaboration with UNICEF
and ICCIDD, has validated new indicators with public health signifi-
cance. This progression has necessitated this new, third edition of these
guidelines.
New indicators of thyroid function have been included, such as meas-
urement of thyroid volume by ultrasound, as there are new international
reference standards, and the measurement of serum thyroglobulin for
which thresholds have been validated to differentiate normal status from
deficiency. In addition, new iodine requirements for pregnant and lac-
tating women have been provided, which results in an increased median
urinary iodine concentration to defi ne a public health problem in preg-
nant women. Lastly, the programmatic criteria to assess progress towards
the elimination of iodine deficiency were revised in light of experience
accumulated in the field.
As a fi rst step to revise the guidelines, experts on iodine were com-
missioned to review and update various sections of the previous version
of the document published in 2000. The ensuing updated sections were
then used as the background document for an expert technical consul-
tation held in Geneva, Switzerland from 22–23 January 2007, with the
objective of conducting a critical analysis of the revised sections, and of
subsequently developing a new document. This revised version was then
distributed widely; not only to participants in the consultation, but also
to other experts in IDD prevention and control whose helpful comments
and suggestions are reflected herein.
Salt iodization is currently the most widely used strategy to control
and eliminate IDD. However, to be fully effective in correcting iodine
deficiency, salt must not only reach the entire affected population (in
particular those groups that are the most susceptible, pregnant women
and young children) but it also needs to be adequately iodized. This is
why these guidelines emphasize process indicators; in particular, those
related to the monitoring of iodized salt at the production and/or impor-
vii
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
tation levels, and iodized salt use in the population. Such monitoring
necessarily involves both governments and the salt industry, requiring
close collaboration between the public and private sectors.
Impact indicators are meant to assess the magnitude of IDD as a
public health problem and to monitor the effects of the intervention on
the iodine status of a population. This manual recommends the use of
urinary iodine to monitor impact. Blood TSH and thyroglobulin may
also be useful for assessing impact, but their use is still limited due to
their cost. The measurement of thyroid size by palpation or ultrasound
was useful initially, but is less useful once salt iodization is established.
For each impact indicator, this manual provides information on biological
features, methods of measurement, and criteria for selecting those
methods and the interpretation of results. The statistical methodology
employed to carry out a survey is also described.
IDD elimination is achieved only if salt iodization can be sustained.
The fi nal chapter addresses this issue, and provides criteria to determine
whether a programme of IDD control is sustainable.
This document is intended primarily for managers of national
programmes dealing with the prevention and control of micronutrient
malnutrition, as well as for policy makers. We hope that the information
included in this manual will be useful, and that it will contribute to our
common goal of the elimination of IDD.
Bruno de Benoist
Coordinator
Micronutrient Malnutrition Unit
Department of Nutrition for Health Development
World Health Organization
Gerald Burrow
Chairman
International Council for Control of Iodine Deficiency Disorders
Werner Schultink
Chief, Nutrition Section
United Nations Children’s Fund
viii
1 Introduction
1
Universal salt iodization (USI) is defi ned as when all salt for human and animal con-
sumption is iodized to the internationally agreed recommended levels.
1
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Target audience
This book is aimed primarily at IDD programme managers and others
in government who are involved in the implementation of IDD control
programmes. It is also aimed at the salt industry and all others involved
in IDD elimination.
2
1. INTRODUCTION
1.2 Definitions
Iodine deficiency disorders (IDD) refer to all of the consequences of
iodine deficiency in a population that can be prevented by ensuring that
the population has an adequate intake of iodine. For further details, see
Chapter 2.
Indicators are used to help describe a situation that exists, and can
be used to track changes in the situation over time. Indicators are usu-
ally quantitative (i.e. measurable in some way), but they may also be
qualitative.
Monitoring is the process of intermittently collecting and analysing
information about a programme for the purpose of identifying prob-
lems, such as non-compliance, and taking corrective action so as to meet
stated objectives.
Evaluation is a process that attempts to determine as systematically
and objectively as possible the relevance, effectiveness, and impact of
activities relevant to their objectives (4).
3
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
• Are there any groups in the population that are not reached by
iodized salt, and thus require attention?
• What is the relative contribution to iodine intake from table salt
versus iodized salt used in the food industry?
• What impact are salt iodization and other interventions having on
the iodine status of the population?
In some countries there is still inadequate information on IDD, and pro-
grammes have not yet been implemented. Here the questions may be:
• Have IDD been eliminated as a public health problem?
• What is the prevalence of IDD in specific population groups (preg-
nant women, infants) based on geographical, administrative, or
physiological criteria?
• What steps are necessary to address IDD, such as a salt situation
analysis?
Answering these questions requires different approaches to gathering
data. It is therefore very important to be quite clear about the purpose of
a particular survey. See Annex 6 for guidelines to assess IDD national
programmes.
4
1. INTRODUCTION
5
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
6
2. IDD AND THEIR CONTROL, AND GLOBAL PROGRESS IN THEIR ELIMINATION
7
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
• In coastal areas;
• In large cities;
• In highly developed countries;
• Where iodine deficiency has been considered to have been elimi-
nated.
In recognition of a much wider occurrence of IDD than previously
thought, certain countries have come to regard the whole country as
being at risk of iodine deficiency and therefore the entire population as
a target for IDD control with iodized salt. The need for continued vigi-
lance is underlined, as is the importance of all countries carrying out
periodic urinary iodine surveys.
8
2. IDD AND THEIR CONTROL, AND GLOBAL PROGRESS IN THEIR ELIMINATION
I, Me
r U TSH t. asu
ato natal in sal ce
i n i
d eoc
e l r a n Ide re ID
v ntif Dp
act te, n e le ssu y r
Imptre ra iodin ality A erage Ass popu evale
o i r u v ess la nc
g
TG, indica l and
t o Q t co sal tion a e (UI
sal t si )
s t r o z e d tua t risk
ces on odi tion
Pro ality C old i
Qu ouseh
H
Monitoring Assessment
evaluation
Communication
Requires: to health
• involvement of professionals and
salt industry Implementation Dissemination public on
of findings importance of
• training
benefits of IDD
• public education elimination
Achieving Planning
political will
Re
lob quires of a es ed;
b i
opi ying o ntens n a tioninclud involv
nio f p ive rdi at rs
n le olit ed
ade icia uca coo e th ecto e
rs o ns a tion n der k forc f all s privat
U tas s o d
f al nd o and e n
l le the
vel r IDD ntativ blic a
s e s e pu
r
rep
a
Adapted from BS Hetzel, 1994 (11).
9
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
10
2. IDD AND THEIR CONTROL, AND GLOBAL PROGRESS IN THEIR ELIMINATION
11
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
basic facts has to be repeated, with the inevitable changes over time in
Ministries of Health and the public heath community and salt produc-
ers. Otherwise, a successful programme will lapse, as has occurred in a
number of countries.
2.5 Sustainability
The progress made with IDD programs in the past decade reflects pro-
gram maturation, and raises the question of how well these programs
will be sustained into the future. IDD cannot be eradicated in one great
global effort like smallpox and, hopefully, poliomyelitis, since these are
infectious diseases with only one host: man. Once eliminated, they can-
not come back. By contrast, IDD is a nutritional deficiency that is prima-
rily the result of deficiency of iodine in soil and water. IDD can therefore
return at any time after their elimination if program success is not sus-
tained. Indeed, there is evidence that iodine deficiency is returning to
some countries where it had been eliminated in the past (13).
Ideally, salt iodization programs ensure that there is adequate iodine
12
2. IDD AND THEIR CONTROL, AND GLOBAL PROGRESS IN THEIR ELIMINATION
intake for the entire population, and the cost of iodization is included
as part of the cost of doing business within the salt industry. The IDD
program in this case simply needs to monitor the situation.
In reality, even with mature salt iodization programs with high cover-
age, programs remain vulnerable to changes in the salt industry, chang-
es in political will, and changes in awareness or consumer acceptance.
Thus, it is important to monitor the overall programmatic indicators as
well as measures of salt iodization and impact to ensure that achieve-
ments are sustained.
Chapter 6 describes indicators of programme sustainability, includ-
ing programmatic indicators. These fall generally into two categories:
1) measures of achievement in salt iodization and iodine status; and 2)
measures of ongoing political support and programme strength.
Monitoring achievement
Sustainable programmes must have a monitoring system that provides
basic information about salt iodization, and about population iodine sta-
tus. This includes monitoring the proportion of households using iodized
salt adequate to meeting iodine intake needs, and assessing iodine sta-
tus through population-based median urinary iodine levels. Monitoring
needs to provide information on where problems may arise at different
levels in the salt iodization production and distribution system that might
contribute to less-than-optimal iodine status. This includes measures
of quality assurance at production facilities, or measures of compliance
with government requirements for imported salt.
When the monitoring system is robust, corrective measures are taken
to ensure that iodized salt use provides adequate intake, and this is con-
fi rmed by periodic population assessment, including understanding the
status of pregnant and lactating women.
13
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
strong partnership with the salt industry, with evidence of their partici-
pation reflected in sound quality assurance measures and absorption of
the cost of potassium iodate into the cost of doing business. And there
should be, as noted above, mechanisms for adequate monitoring of salt
and iodine status, periodic reporting, and establishment of an ongoing
national database to track sustained progress.
With these elements in place, and with achievement of high iodized
salt use, programs have reduced vulnerability, and are likely to be sus-
tained.
1
UI between 100 µg/l and 199 µg/l
2
UI between 200 µg/l and 299 µg/l
3
UI above 300 µg/l
14
2. IDD AND THEIR CONTROL, AND GLOBAL PROGRESS IN THEIR ELIMINATION
15
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
16
3 Indicators of the
salt iodization process
1
Potassium iodate and potassium iodide have a long-standing and widespread history
of use for fortifying salt without apparent adverse health effects. Potassium iodate has
been shown to be a more suitable substance for fortifying salt than potassium iodide
because of its greater stability, particularly in warm, damp, or tropical climates. In ad-
dition, no data are available indicating toxicological hazard from the ingestion of these
salts below the level of Provisional Maximum Tolerable Daily Intake or PMTDI (15).
17
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Recommendations
WHO/UNICEF/ICCIDD (19) recommend that, in typical circum-
stances, where the iodine lost from salt is 20% from production site to
1
P Joost, personal communication, December 2006.
18
3. INDICATORS OF THE SALT IODIZATION PROCESS
19
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Titration method
The titration method requires the use of a small laboratory equipped
with some basic instruments, such as a precision scale, a burette, glass-
ware, and pipettes. Additional equipment, such as a magnetic stirrer and
dispensers, will save time and optimize the analytical procedure.
Basically, iodine analysis by titration involves the preparation of four
solutions and a standard solution which will last for variable periods of
time, and then determining the iodine concentration in a salt solution
by adding the pre-made reagents/solutions followed by the titration step.
The iodine content of salt is determined by liberating iodine from salt and
titrating the iodine with sodium thiosulfate using starch as an external
20
3. INDICATORS OF THE SALT IODIZATION PROCESS
21
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
22
Figure 2 A monitoring and evaluation system for salt iodization
Certificate of Quality
Food fortified with iodized salt Iodized salt (Food Control and
National or imported Customs)
23
Customs) Commercial monitoring Verification of Legal Compliance
(Food Control and Units of Standards
(at retail stores)
and/or Consumer Protection)
Quality auditing with
conformityAssessment
(Food Control/witnesses)
checked at least once. Rapid test kits can be used more frequently to
check that the addition of iodine has not been interrupted.
It is recommended that wherever possible adequate staff at a produc-
tion plant should be trained and their skills standardized to determine
the iodine concentration accurately using the titration method. Fur-
thermore, key persons at each production site, including the managers,
should be aware of the detrimental consequences of iodine deficiency
and excess, as well as the health benefits of correctly iodized salt.
Results should be recorded and plotted in a quality assurance chart.
When levels are not satisfactory, immediate corrective action should be
taken and that action entered into the record book.
Because production methods and factory sizes vary so widely, it is
beyond the scope of this manual to defi ne this process in any greater
detail. Whatever the method adopted, the process, combined with exter-
nal quality assurance measures, should result in salt that has an iodine
level within the upper and lower range established by regulations. In
other words, internal and external quality control should ensure that the
level of iodine is in the range stipulated by national regulations, that is
both effective to control iodine deficiency and safe with regard to exces-
sive iodine intake.
When importers and distributors procure salt, they have the respon-
sibility to either ensure that it meets specifications as stipulated in the
requirements, or to ensure that these are met before salt goes out to the
wholesale or retail market. This implies that they should have a quality
assurance system that includes salt iodine titration measurements.
If the salt they receive is not up to standard, they will need to have
their own iodization facility. All salt should be distributed in polyethyl-
ene bags with appropriate labels.
24
3. INDICATORS OF THE SALT IODIZATION PROCESS
25
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
26
3. INDICATORS OF THE SALT IODIZATION PROCESS
Questions on iodized salt use and salt testing have been included in the
UNICEF Multiple Indicator Cluster Surveys (MICS) and in the Demo-
graphic and Health Surveys. Some countries have successfully added
household salt testing to other national surveys, e.g., to either nutrition
surveys or surveys that collect key economic and census data. These sur-
veys provide estimates of the proportion of the population with iodized
salt coverage, and identify areas where there is low use of iodized salt
and/or where all the salt is non-iodized.
National surveys can be costly, and a community-based method may
be possible on a more regular basis. This approach may be organized in
the community or through the schools, particularly in areas with high
rates of school enrolment. Providing salt testing kits to environmental
health officers, community midwives, nutrition officers, schoolteach-
ers, mayors, and other government workers responsible for community
health, has been helpful in this process. These approaches are very effec-
tive communication and awareness tools, particularly when this aware-
ness is linked to action. This action could involve approaching the salt
producers or distributors and directly requesting them to supply iodized
salt.
Depending on the sampling methods and survey design adopted, it
may be possible for monitoring at the household level to provide results
that allow for visual representation of variations of coverage and provide
a basis for targeting resources and focusing interventions in areas where
they are most needed. Monitoring at this level should be followed by
specific action to identify further reasons for low iodized salt usage, and
should result in a range of actions to correct the problem.
Finally, the occurrence of parallel markets of non-iodized salt has
frequently been a barrier to achieving USI. National cross-sectional
household surveys and community monitoring have often been useful
in identifying such salt and in developing strategies to address the prob-
lem.
27
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
4 Indicators of impact
4.1 Overview
Assessment of thyroid size by palpation is the time-honoured method
of assessing IDD prevalence. However, because of the lack of sensitivity
to acute changes in iodine intake, this method is of limited usefulness
in assessing the impact of programmes once salt iodization has com-
menced. In this case, urinary iodine is the most useful indicator because
it is reflective of the current intake of iodine in the diet (23).
Since most countries have now started to implement IDD control pro-
grammes, urinary iodine rather than thyroid size is emphasized in this
manual as the principal indicator of impact. Thyroid size is more useful
in baseline assessments of the severity of IDD, and also has a role in the
assessment of the long-term impact of control programmes.
The introduction of ultrasonography for the assessment of thyroid
size has been a significant development. In areas of mild to moderate
IDD, measurement of thyroid volume using ultrasound is preferable to
palpation for grading goitre. New international reference values for thy-
roid volume by ultrasound have recently become available and can be
used for goitre screening in the context of IDD monitoring (24).
Two other indicators are included in this chapter: thyroid stimulating
hormone (TSH), and thyroglobulin (Tg). While TSH levels in neonates
are particularly sensitive to iodine deficiency, and although difficulties in
interpretation remain, there is a potential future for the use of neonatal
TSH in the identification of IDD and their control; although the cost of
implementing a TSH screening programme is too high for most devel-
oping countries. Measurement of Tg in children is a sensitive indicator
of iodine status and improving thyroid function after iodine repletion. A
standardized dried blood spot Tg assay has been developed and can be
used for assessing and monitoring iodine nutrition in the field (25).
28
4. INDICATORS OF IMPACT
Feasibility
Acceptance of this indicator is very high, and casual urine specimens are
easy to obtain. Urinary iodine assay methods are not difficult to learn
or use, but meticulous attention is required to avoid contamination with
iodine at all stages. Special laboratory areas, glassware, and reagents
should be set aside solely for this determination.
In general, only small amounts (0.5–1.0 ml) of urine are required,
although the exact volume depends on the method. Some urine should
also be kept in reserve for replicate testing or for external quality con-
trol. Samples are collected in small cups and transferred to tubes, which
should be tightly sealed with screw tops. They do not require refrigera-
tion, addition of preservative, or immediate determination in most meth-
ods. They can be kept in the laboratory for months or more, preferably
in a refrigerator to avoid unpleasant odour.
Evaporation should be avoided, because this process artificially
increases the concentration. Samples may safely be frozen and refrozen,
but must be completely defrosted before aliquots are taken for analysis.
Many analytical techniques exist, varying from very precise measure-
ment with highly sophisticated instruments, to semi-quantitative ‘low
tech’ methods that can be used in regional, country, or local laborato-
ries. Most methods depend on iodine’s role as a catalyst in the reduction
of ceric ammonium sulfate (yellow colour) to the cerous form (colour-
less) in the presence of arsenious acid (the Sandell-Kolthoff reaction). A
digestion or other purification step using ammonium persulfate or chlo-
ric acid is necessary before carrying out this reaction, to rid the urine of
interfering contaminants.
A brief description of some of the methods introduced in this section
is presented in the following pages.
29
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Other methods
A modification of method B uses the redox indicator ferroin and a stop-
watch instead of a spectrophotometer to measure colour change (28).
Urine is digested with chloric acid and colour changes in batches of sam-
ples measured relative to standards of known iodine content. This places
samples in categories (e.g., below 50 µg/l, 50–100 µg/l, 100–200 µg/l,
etc.) that can be adjusted to desired levels. This method is currently
being adapted to ammonium persulfate digestion.
Another, semi-quantitative method is based on the iodide-catalysed
oxidation of 3,3’,5,5’-tetramethylbenzidine by peracetic acid/H 2O2 to
yield coloured products that are recognized on a colour strip indicating
three ranges: <100 µg/l, 100–300 µg/l, and >300 µg/l (29). Interfering
substances are removed by pre-packed columns with activated charcoal.
Analyses must be run within two hours, and the procedure requires the
manufacturer’s pre-packed columns.
In still another method, samples are digested with ammonium persul-
fate on microplates enclosed in specially designed sealed cassettes and
heated to 110 °C (30). Samples are then transferred to another micro-
plate and the ceric ammonium sulfate reduction reaction carried out and
read on a microplate reader. Field tests are promising: up to 400 urine
samples can be analysed in one day, depending on manufacturers’ sup-
plies.
30
4. INDICATORS OF IMPACT
Choice of method
Criteria for assessing urinary iodine methods are reliability, speed, tech-
nical demands, complexity of instrumentation, independence from sole-
source suppliers, availability of high quality reagents, safety, and cost.
The choice among the above and other methods depends on local needs
and resources. Large central laboratories processing many samples may
prefer ‘high-tech’ methods, while smaller operations closer to the field
may fi nd the simplest methods more practical.
Due to the potential hazards of chloric acid, method A (see Annex 3)
using ammonium persulfate is currently recommended. It can adequate-
ly replace the chloric acid method, since the main difference is the sub-
stitution of ammonium persulfate for chloric acid in the digestion step.
Results are comparable.
The other methods described above show promise but are not yet
fully tested.
1
IRLI Network was jointly established by CDC, WHO, UNICEF, ICCIDD and MI to
identify laboratories to serve as effective resources for their regions, thus strengthening
the capacity of laboratories throughout the world to accurately measure iodine in urine
and salt. The network currently includes 12 laboratories (Australia, Belgium, Bulgaria,
Cameroon, China, Guatemala, India, Indonesia, Kazakhstan, Peru, the Russian Fed-
eration, South Africa). http://iodinenetwork.net/Resources_Lab.htm
2
EQUIP uses laboratory quality assurance as a tool for eliminating IDD worldwide.
It is a CDC standardization program designed to provide urinary iodine laboratories
with an independent assessment of their analytical performance. The program assists
laboratories to monitor the degree of variability and bias in their urinary iodine assay.
IRLI laboratories are invited to participate in the EQUIP program. http://www.cdc.
gov/nceh/globalhealth/projects/labactivites.htm
31
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Performance
Most of the above methods perform reliably, although some of the newer
ones need further testing as of this date. With appropriate dilutions, they
can be extended upward to examine whatever range is desired. The coef-
ficient of variation is generally under 10% for all methods. Proper train-
ing is necessary but not complicated.
Since casual specimens are used, it is desirable to measure a suffi-
cient number from a given population to allow for varying degrees of
subject hydration and other biological variations among individuals, as
well as to obtain a reasonably narrow confidence interval (see Annex 4).
In general, 30 urine determinations from a defi ned sampling group are
sufficient.
Interpretation
Simple modern methods make it feasible to process large numbers of
samples at a low cost and to characterize the distribution according to
different cut-off points and intervals. The cut-off points proposed for
classifying iodine nutrition into different degrees of public health signifi-
cance are shown in Table 4 and Table 5.
The median value for the sampled population is the most commonly
assessed indicator. Urinary iodine values from populations are usually
not normally distributed. Therefore, the median rather than the mean
should be used as the measure of central tendency. Likewise, percentiles
rather than standard deviations should be used as measures of spread.
Frequency distribution curves can also be very useful for full interpreta-
tion, particularly if there is salt iodine level data available for the same
population.
In children and non-pregnant women, median urinary iodine con-
centrations of between 100 µg/l and 299 µg/l defi ne a population which
has no iodine deficiency.1 In addition, not more than 20% of samples
should be below 50 µg/l. In non-pregnant, non-lactating women, a uri-
nary iodine concentration of 100 µg/l corresponds roughly to a daily
iodine intake of about 150 µg under steady-state conditions.
During pregnancy, median urinary iodine concentrations of between
150 µg/l and 249 µg/l defi ne a population which has no iodine deficiency
(6).
Establishing the ideal range of values for urinary iodine is difficult.
Historically, schoolchildren were assessed by palpation, establishing a
pre-intervention baseline for the prevalence of IDD. This population
1
By defi nition, when the median is 100 µg/l, at least 50% of the samples will be lower
than 100 µg/l.
32
4. INDICATORS OF IMPACT
was also sampled for urinary iodine, thus establishing a normal range.
This normal range has been extrapolated to the full population. It may
be more logical to sample women of reproductive age, or adolescent girls
– thus providing more information on populations that may include
those with or on the verge of greater need. The upper limit of the recom-
mended range for these populations reflects concern about the risk of
hyperthyroidism when high levels are introduced to a previously endem-
ic population.
Recent data have suggested that the normal range for pregnant and
lactating women should reflect their additional need and the risk that
these needs may not be met if population levels are too low. However,
this leaves a relatively narrow range for a median UI level that will both
meet the needs for pregnant/lactating women, and not be excessive for
33
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
the remainder of the population. This guide provides the best current
estimates for the optimal values to meet the overall population needs.
Urinary iodine concentration is currently the most practical biochem-
ical marker for iodine nutrition when carried out with appropriate tech-
nology and sampling. This approach assesses iodine nutrition only at the
time of measurement, whereas thyroid size reflects iodine nutrition over
months or years. Therefore, even though populations may have attained
iodine sufficiency on the basis of median urinary iodine concentration,
goitre may persist, even in children.
With rapid global progress in correcting iodine deficiency, examples
of iodine excess are being recognized, particularly when salt iodization
is excessive and poorly monitored (20). Tolerance to high doses of iodine
is quite variable, and many individuals ingest amounts of several milli-
grams or more per day without apparent problems. The major epidemio-
logical consequence of iodine excess is iodine-induced hyperthyroidism
(IIH) (24,31). This occurs more commonly in older subjects with pre-
existing nodular goitres, and may occur even when iodine intake is with-
in the normal range.
Iodine intakes above 300 µg/l per day should generally be discouraged,
particularly in areas where iodine deficiency has previously existed. In
these situations, more individuals may be vulnerable to adverse health
consequences, including iodine-induced hyperthyroidism and autoim-
mune thyroid diseases.
In populations characterized by long-standing iodine deficiency
and a rapid increase in iodine intake, median values for urinary iodine
above 200 µg/l (and in pregnant women, above 250 µg/l) are not recom-
mended because of the possible risk of iodine-induced hyperthyroidism.
This adverse condition can occur during the 5 to 10 years following the
introduction of iodized salt (24,31). Beyond this period of time, median
values up to 300 µg/l have not demonstrated side-effects, at least not
in populations with adequately iodized salt. In schoolchildren, urinary
iodine concentrations >500 µg/l are associated with increasing thyroid
volume, which reflects the adverse effects of chronic iodine excess (32).
34
4. INDICATORS OF IMPACT
Feasibility
Palpation of the thyroid is particularly useful in assessing goitre preva-
lence before the introduction of any intervention to control IDD, but
much less so in determining impact. Costs are associated with mounting
a survey, which is relatively easy to conduct, and training of personnel.
These costs will vary depending upon the availability of health care per-
sonnel, accessibility of the population, and sample size. Feasibility and
performance vary according to target groups, as follows:
Neonates: It is neither feasible nor practical to assess goitre among
neonates, whether by palpation or ultrasound. Performance is poor.
School-age children (6–12 years): This is the preferred group, as
it is usually easily accessible. However, the highest prevalence of goitre
occurs during puberty and childbearing age. Some studies have focused
on children 8 to 10 years of age.
There is a practical reason for not measuring very young age groups.
The smaller the child, the smaller the thyroid, and the more difficult it
is to perform palpation.
If the proportion of children attending school is low, schoolchildren
may not be representative (Annex 4). In these cases, spot surveys should
be conducted among those who attend school and those who do not, to
ascertain if there is any significant difference between the two.
Alternatively, children can be surveyed in households. For further dis-
cussion, see Chapter 5 on survey methods.
Adults: Pregnant and lactating women are of particular concern. Preg-
nant women are a prime target group for IDD control activities because
they are especially sensitive to marginal iodine deficiency. Often they are
relatively accessible given their participation in antenatal clinics. Women
of childbearing age – 15 to 44 years – may be surveyed in households.
35
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Technique
The subject to be examined stands in front of the examiner, who looks
carefully at the neck for any sign of visible thyroid enlargement. The
subject is then asked to look up and thereby to fully extend the neck.
This pushes the thyroid forward and makes any enlargement more
obvious.
Finally, the examiner palpates the thyroid by gently sliding their
own thumb along the side of the trachea (wind-pipe) between the
cricoid cartilage and the top of the sternum. Both sides of the trachea
are checked. The size and consistency of the thyroid gland are carefully
noted.
If necessary, the subject is asked to swallow (e.g. some water) when
being examined – the thyroid moves up on swallowing. The size of each
lobe of the thyroid is compared to the size of the tip (terminal phalanx)
of the thumb of the subject being examined.1 Goitre is graded according
to the classification presented in Table 6.
The specificity and sensitivity of palpation are low in grades 0 and 1 due
to a high inter-observer variation. As demonstrated by studies of experi-
enced examiners, misclassification can be high.
Interpretation
Table 7 gives the epidemiological criteria for establishing IDD severity,
based on goitre prevalence in school-age children. The terms mild, mod-
erate, and severe are relative and should be interpreted in context with
information from other indicators.
1
Another method is to stand behind the subject with the neck in the neutral position and
hold the fi ngers (not thumb) over the area of the gland. The person is asked to swallow
and the gland is palpated by the fi ngers as it glides up. This is repeated on each side of
the neck.
36
4. INDICATORS OF IMPACT
Feasibility
Portable (weight 12–15 kg) ultrasound equipment with a 7.5 MHz
transducer currently costs about US $15 000. A source of electricity is
needed, and the operator needs to be specially trained in the technique.
Differences in technique (e.g. the pressure applied with the transducer)
and estimation of thyroid anatomy (e.g. inclusion of the thyroid isthmus
and/or capsule thickness) can result in high inter-observer variability.
37
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Interpretation
Results of ultrasonography from a study population should be compared
with reference data (33). Reference values for thyroid volume measured
by ultrasonography in schoolchildren of iodine-sufficient populations
are shown in Table 8. These are presented as a function of age, sex, and
body surface area (BSA) in order to take into account the differences in
body development among children of the same age in different countries.
This approach is potentially useful in countries with a high prevalence
of child growth retardation due to malnutrition with both stunting (low
height-for-age) and underweight (low weight-for-age).
An advantage of the thyroid volume-for-BSA is that the age of the
child is not required, which in some populations is not known with cer-
tainty. A limitation of the thyroid volume-for-BSA is that it requires the
collection of weights and heights: in severely malnourished populations
of schoolchildren, 10% or more may have a BSA below the lowest BSA
cut-off of 0.7.
The thyroid volume references proposed here are applicable for goi-
tre screening only if thyroid volume is determined by the standardized
method described in Annex 2.
38
4. INDICATORS OF IMPACT
39
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
neonates with TSH elevations which are 20 mIU/l whole blood or higher,
the availability of TSH assays sensitive to 5 mlU/l permits detection of
mild elevations above normal. This permits detection of transient hyper-
thyrotopinemia. To be broadly applicable in a population, the screening
must be universal, and not omit children born in remote or impoverished
areas. For countries and regions that already have a system of universal
neonatal screening with a sensitive TSH assay in place, the data can be
examined and transient iodine deficiency recognized, usually without
further surveying.
Feasibility
Serum TSH is widely used in the field of thyroidology as a sensitive
marker for both hypothyroidism and hyperthyroidism. Methods for
determining TSH concentrations, from either dried whole blood spots
on fi lter paper or from serum, are well established and widely available.
Typically, a few drops of whole blood are collected on fi lter paper from
the cord or by prick of the heel or other site.
It is essential that sterile equipment be used, either lancets for blood
spot collection or needles and syringes for collecting whole blood from
which the serum is separated. Standard procedures for handling blood
products or objects contaminated with blood should be followed. The
risk of contracting HIV or hepatitis infection from dried blood spots is
extremely low.
Some experimental data suggest normal values for cord blood are
higher than those for heel prick blood. Blood spots, once dried, are sta-
ble. They can be stored in a plastic bag and transported even through
normal postal systems and are usually stable for up to six weeks.
It must be emphasized that the primary purpose of screening pro-
grammes is to detect congenital hypothyroidism, and its use as an indica-
tor of iodine nutrition will be a spin-off. Hence, the only additional cost
will be for data analysis. It is not recommended that a neonatal screening
programme be set up solely to assess community iodine deficiency. Less
expensive means for obtaining this information exist.
TSH screening is inappropriate for developing countries where health
budgets are low. In such countries, mortality among children under five
is high due to nutritional deficiencies and infectious diseases, and screen-
ing programmes for congenital hypothyroidism are not cost effective.
Performance
A variety of kits for measuring TSH are available commercially in devel-
oped countries. Most have been carefully standardized, and perform
adequately. Assays that utilize monoclonal antibodies, which can detect
40
4. INDICATORS OF IMPACT
TSH as low as 5 mIU/l in whole blood spots, are more useful for recog-
nizing iodine deficiency.
Interpretation
Permanent sporadic congenital hypothyroidism, with extremely elevated
neonatal TSH, occurs in approximately one of 4000 births in iodine-
sufficient countries. Other than infrequent cases of goitrogen exposure,
iodine deficiency is the only significant factor to increase this incidence.
The increase in the number of neonates with moderately elevated
TSH concentrations (above 5 mlU/l whole blood) is proportional to the
degree of iodine deficiency during pregnancy. It may be higher than
40% in severe endemic areas. When a sensitive TSH assay is used on
samples collected three to four days after birth, a <3% frequency of TSH
values >5 mlU/l indicates iodine sufficiency in a population (34).
Interpretation is complicated when antiseptics containing beta-
iodine, such as povidone iodine (Betadine™), are used for cleaning the
perineum prior to delivery or even the umbilical area of the baby. Beta-
iodine increases TSH levels in the neonate in both cord blood and heel
prick specimens.
Interpretation
Standard reference material for the DBS Tg assay is now available
from WHO. It is stable when stored for up to one year at temperatures
≤ -20 °C. An international reference range for DBS Tg has been estab-
lished in iodine-sufficient five to 14 year-old children that can be used for
monitoring iodine nutrition. The DBS Tg reference interval for iodine-
sufficient school-age children is 4–40 µg/l.
41
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Performance
DBS Tg correlates well with urinary iodine and thyroid size (35,36), the
other recommended indicators for monitoring iodine status in popula-
tions. It complements these tests, and can be used in conjunction with
urinary iodine to measure recent iodine intake, and thyroid volume to
assess long-term anatomic response.
Feasibility
The method is simple and robust. A drop of whole blood from a fi nger
stick (or a venipuncture sample) is spotted directly onto good-quality
fi lter paper.1 The spots are allowed to dry at room temperature (~20 °C),
and then stored in sealed low-density polyethylene bags; preferably
refrigerated at 4 °C, but they also can be stored for several weeks at cool,
dry room temperatures before analysis.
1
An example of the type of paper that can be used is Grade 903 Filter Paper produced
by Schleicher & Schuell; Einbeck, Germany.
42
Table 9 Indicators of impact at population level: summary
MONITORING AGE GROUP FOR ADVANTAGES DISADVANTAGES
INDICATOR (UNITS) ASSESSMENT
Median urinary School-age – Spot urine specimens are easy to obtain – Assesses iodine intake only over the past few days
iodine children and – The most practical biochemical marker for iodine nutrition, when – Meticulous laboratory practice is required to avoid
concentration pregnant carried out with appropriate technology and sampling contamination with iodine
(µg/l) women – Feasible to process large numbers of samples at low cost – A sufficiently large number of samples must be collected to
– Cut-off points proposed for classifying iodine nutrition into allow for varying degrees of subject hydration and other
different degrees of public health significance are well established biological variations among individuals
– External quality control program in place – Not valuable for individual assessment
Goitre rate School-age – Simple and rapid screening test – Specificity and sensitivity of palpation are low in grades 0
assessed by children – Requires no specialized equipment and 1 due to a high inter-observer variation
palpation (%) – Responds slowly to changes in iodine intake
Goitre rate School-age – A more precise measurement of thyroid volume compared with – Expensive equipment and a source of electricity is needed
assessed by children palpation – operator needs to be specially trained in the technique
ultrasound (%) – Safe, non-invasive – Responds slowly to changes in iodine intake
43
– International reference values for thyroid volume in schoolchildren
are available as a function of age, sex, and body surface area
TSH Newborns – Measures thyroid function at a vulnerable age when iodine – Not recommended to be set up solely to assess community
4. INDICATORS OF IMPACT
(mIU/l) deficiency directly affects the developing brain iodine deficiency due to expense
– If screening programs to detect congenital hypothyroidism is in – Cannot be used when antiseptics containing iodine are used
place then only additional cost will be for data analysis during delivery
– Collection by heel stick and storage on filter paper is simple – Requires use of a standardized, sensitive assay
– Blood spots can be stored for several weeks at cool, dry room – Should be taken either from the cord at delivery or by heel
temperatures prick at least 48 hours after birth to avoid physiological
newborn surge
Tg School-age – Collection by finger stick and storage on filter paper is simple – Expensive immunoassay
(µg/l) children – Can be stored for several weeks at cool, dry room temperatures, – Requires laboratory infrastructure
so sampling practical even in remote areas
– Measures improving thyroid function within several months after
iodine repletion
– Standard reference material is now available, but needs to be validated
– An international reference range has been established
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
5 Monitoring and
evaluation methods
5.1 Overview
This book has so far dealt with what should be measured, i.e. the indica-
tors of process and impact, and the technical details of their assessment.
This chapter describes how to monitor and evaluate these indicators in
the field.
This chapter includes methodologies for monitoring the process of salt
iodization at the population level to complement the discussion in Chap-
ter 3, and provides some details on monitoring methods for assessing
the impact of programs on iodine status and thyroid function. For some
monitoring activities such as performing surveys at schools or in house-
holds, both salt and impact indicators can be assessed at the same time,
thus providing a more comprehensive picture of the situation.
Attention to proper survey methodology will result in representative
results from the population. Careful attention to monitoring methods
will assure the monitoring is carried out as efficiently as possible.
44
5. MONITORING AND EVALUATION METHODS
45
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
46
5. MONITORING AND EVALUATION METHODS
tine test kits will provide an estimate of the percent of households using
salt with no iodine, but will not provide accurate information on the per-
cent using adequately iodized salt, or information on salt with excessive
iodine. Thus, titration or another quantitative method should be per-
formed on at least a sub-sample of households for any coverage survey.
47
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
48
5. MONITORING AND EVALUATION METHODS
49
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
50
5. MONITORING AND EVALUATION METHODS
51
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
52
6. INDICATORS OF THE SUSTAINABLE ELIMINATION OF IDD
53
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Salt iodization
Proportion of households using
adequately iodized salt >90%
Urinary iodine
Median in the general population 100–199 µg/l
Median in pregnant women 150–249 µg/l
Programmatic indicators Attainment of eight out of 10 indicators specified in
Section 6.3
54
Annexes
ANNEX 1
57
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
58
ANNEX 1. TITRATION METHOD FOR DETERMINING SALT IODATE AND SALT IODIDE CONTENT
A1.1.3 Procedure
Sampling of salt
Prior to taking a 10 g or 50 g salt sample for analysis, salt should be
thoroughly mixed, preferably in zip-lock bags or appropriate contain-
ers to ensure that the iodine is homogeneously distributed in the salt.
Usually 10 g iodated salt is dissolved in 50 ml distilled water. Optional:
50 g iodated salt could be thoroughly dissolved in 250 ml distilled water,
from which an aliquot of 50 ml could be analysed as mentioned in the
titration step below, without adjusting the concentrations of the reagents
or calculation.
Titration step
Once the salt is dissolved in the measured amount of water, sulfuric acid
(1–2 ml) and potassium iodide (5 ml) is added to the salt solution, which
in the presence of iodine will turn yellow. The reaction mixture is then
kept in a dark place (with no exposure to light) for five to 10 minutes to
reach the optimal reaction time, before titrated with sodium thiosulfate
using starch (2 ml) as the indirect indicator. The concentration of iodine
in salt is calculated based on the titrated volume (burette reading) of
sodium thiosulfate according to the formula mentioned below, or alter-
natively it could be read off a pre-calculated table for the specific method
(e.g. method: 10 g salt titrated with 0.005N sodium thiosulfate).
Calculation
mg/kg (ppm) iodine = titration volume in ml x 21.15 x normality
of sodium thiosulfate x 1000 / salt sample weight in g
Special note: 0.005 N sodium thiosulfate consumes 0.1058 mg iodine/ml.
Precaution
The 10% potassium iodide reagent stock needs to be protected from
direct light, and the reaction mixture (after the addition of sulfuric acid
and potassium iodide) should be kept in the dark before titration to pre-
vent a side reaction which may occur when these solutions are exposed
to light, causing iodide ions to be oxidized to iodine.
59
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
60
ANNEX 1. TITRATION METHOD FOR DETERMINING SALT IODATE AND SALT IODIDE CONTENT
A1.2.3 Procedure
Sampling
As described in the iodate method.
61
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
ANNEX 2
Trachea
Thyroid gland
L
D
62
ANNEX 2. METHOD FOR DETERMINING THYROID SIZE BY ULTRASONOGRAPHY
A2.3.2 Transducer
• Water-soluble gel is used.
• Transducer held at a 90-degree angle to skin, using only minimal
pressure so as not to distort the gland anatomy.
63
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Width
Depth
Length
64
ANNEX 2. METHOD FOR DETERMINING THYROID SIZE BY ULTRASONOGRAPHY
image which shows the lobe at its greatest length (craniocaudal), and
freeze.
• To obtain the greatest length, because of the inferior convergence of
the lobes, the transducer is often oriented with its superior end slightly
diverging from the midline. Measure the maximal length of the longi-
tudinal section of the lobe.
• Repeat scan on opposite lobe and again measure the maximal length
of the longitudinal section.
• If the length of the gland exceeds the length of the transducer, the
longitudinal measurement is done by splitting the lobe length in two
scans, measuring to an internal (preferable) or external landmark,
and summing the measurements to obtain the length.
65
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
ANNEX 3
A3.2 Equipment
Heating block with a temperature range up to 110°C or above (ven-
ted fume hood recommended, but not essential); spectrophotometer;
thermometer, test tubes (13 x 100 mm); assorted glassware and storage
bottles; pipettes; vortex; magnetic hotplate; magnetic stirrer; analytical
balance or top loader scales with a readability of at least 0.001 g and a
capacity of approximately 250 g.
A3.3 Reagents
1. Ammonium persulfate (H8N2O8S2)
2. Arsenic trioxide (As2O3)
3. Sodium chloride (NaCl)
4. Sulfuric acid (H2SO4)
5. Sodium hydroxide (NaOH)
6. Cerric ammonium sulfate [Ce(NH4)4(SO4)4 ·2H 2O]
7. Deionized water (H2O)
8. Potassium iodate (KIO3)
Use analytical grade chemicals for the preparation of all solutions
A3.4 Solutions
Ammonium persulfate (1.0 mol/l): Dissolve 114.1 g ammonium
persulfate in 500 ml deionized water. Store in darkness; stable for one
month. Keep refrigerated to prevent decomposition.
5 N Sulfuric acid: Slowly add 140 ml concentrated (36 N) sulfuric acid
to about 700 ml deionized water (careful – this generates heat!). When
cool, adjust with deionized water to a fi nal volume of 1 litre.
66
ANNEX 3. METHOD FOR MEASURING URINARY IODINE USING AMMONIUM PERSULFATE (METHOD A)
A3.5 Procedure
1. Allow urine to reach room temperature, then mix urine to suspend
sediment.
67
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
A3.7 Notes
1. This is modified from the former method (method B, see page 30),
substituting ammonium persulfate for chloric acid (more toxic) as the
digestant (26, 27, 40).
2. Since the digestion procedure has no specific end-point, it is essential
to run blanks and standards with each assay to allow for variations in
heating time, etc.
68
ANNEX 3. METHOD FOR MEASURING URINARY IODINE USING AMMONIUM PERSULFATE (METHOD A)
3. The exact temperature, heating time, and cooling time may vary.
However, within each assay, the interval between the time of addition
of ceric ammonium sulfate and the time of the reading must be the
same for all samples, standards, and blanks.
4. With the longer ceric ammonium sulfate incubation and with 15-
second interval additions of ceric ammonium sulfate, up to 120 tubes
can be read in a single assay.
5. The volumes and proportions of samples and reagents can be varied
to achieve different concentrations or a different curve shape, if con-
ditions warrant. If different tube sizes are used, corresponding sized
holes in the heating block are also needed.
6. If necessary, this method could probably be applied without a heating
block, using a water, oil, or sand bath, but this is not recommended.
It is essential that all tubes be uniformly heated and that the tempera-
ture be constant within the range described above.
7. Test tubes can be reused if they are carefully washed to eliminate any
iodine contamination. Durable 13 x 100 mm or 16 x 100 mm culture
tubes with screw caps are recommended (e.g., Corning or KIMAX
tubes).
8. This method can be slighty modified to be suitable for automation,
which allows for colorimetric readings to be done in microtiter plates
and subsequently with a scanner. For example, with the same diges-
tion step, only the ceric ammonium sulfate concentration could be
adjusted to approximately 0.016 or 0.019 mol/l to accomodate the
microtiter plate applications (30).
9. The Centers for Disease Control and Prevention (CDC), Atlanta,
USA provides urinary iodine laboratories the opportunity to partici-
pate in an external quality control program (EQUIP).
69
ANNEX 4
1
Adapted from: Sullivan KM et al. (40)
70
ANNEX 4. METHODOLOGY FOR SELECTION OF SURVEY SITES BY PPS SAMPLING
71
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
72
ANNEX 4. METHODOLOGY FOR SELECTION OF SURVEY SITES BY PPS SAMPLING
73
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
74
ANNEX 4. METHODOLOGY FOR SELECTION OF SURVEY SITES BY PPS SAMPLING
75
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
76
ANNEX 4. METHODOLOGY FOR SELECTION OF SURVEY SITES BY PPS SAMPLING
Other possibilities
In situations where male and female children attend the same school, the
selection of schools and pupils would be the same as discussed above.
In situations where males and females attend separate schools, when a
school of one sex is selected the nearest school of the opposite sex should
also be surveyed.
For example, a survey is to be performed in an area where males and
females attend separate schools. Thirty schools are to be selected, and 20
pupils sampled in each. When an all-male school is visited, information
should be collected on 10 male pupils. Then, the nearest female school is
visited, and information collected on 10 female pupils.
77
ANNEX 5
1
In Microsoft Excel, use the Data Analysis function on the Tools menu, and select
“Rank and Percentile”.
78
ANNEX 5. SUMMARIZING URINARY IODINE DATA: A WORKED EXAMPLE
(range of cells, 0.2)]. The 20th percentile (P20) is 82.4 µg/l and P80
is 191.8 µg/l.
7. The “Descriptive Statistics” function of Data Analysis in Excel pro-
vides all statistics shown: select “Summary Statistics” in the dialogue
box. Note that the mean is much higher than the median, indicating
that the distribution is heavily skewed to the right. This is also shown
by the much greater distance between P80 and the median, compared
to that between P20 and the median.
8. In addition, the data can be shown as a histogram using the “Histo-
gram” function of Data Analysis in Excel. Convenient ranges need
to be chosen for making the frequency distribution, which will be
reflected in the height of each bar of the histogram. 50 µg/l is sug-
gested (i.e., the fi rst bar is 0–49 µg/l, the second 50–99 µg/l, the third
100–149 µg/l, etc.). Appropriate modifications can be made using
“Chart Options” and related functions. The histogram is shown in
Figure 6. A fully detailed description for constructing that histogram
is not given here.
In presenting this distribution, it is important not to misinterpret the
different percentages. A common mistake is to assume that there is defi-
ciency because 33.7% have a UI value <100 µg/l. This is not the correct
interpretation of the median value and distribution statistics. Instead,
this calculation shows the distribution of values around the median val-
ue, and helps determine if there is a large proportion with either very low
or very high values.
These results indicate that there is no iodine deficiency, and that salt
iodization is therefore having the required impact. There is no evidence
of significant over-iodization. No changes are needed on the basis of
these results, but further follow-up is always essential.
79
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
80
ANNEX 5. SUMMARIZING URINARY IODINE DATA: A WORKED EXAMPLE
Table 14 continued
UI(µg/l) VALUE RANK PERCENT DESCRIPTIVE STATISTICS
81
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
0–49 6
50–99 27
100–149 35
150–199 13
200–249 7
250–299 5
300–349 2
350–399 1
400–449 0
450–499 1
500–549 1
550–599 0
40
30
# of samples
20
10
0
0–49 50–99 100– 150– 200– 250– 300– 350– 400– 450– 500– 550–
149 199 249 299 349 399 449 499 549 599
urinary iodine (µg/l)
82
ANNEX 6
Guidelines to assess
IDD national programmes1
A6.1 Background
The iodine deficiency elimination goal was set at the World Summit for
Children in 1990, and subsequently in 1993 when WHO and UNICEF
agreed to recommend USI to each nation where IDD is a public health
problem. The IDD elimination goal was reaffi rmed by multi-sec-
tor and national delegations during the UN Special Session for Chil-
dren (UNGASS) in New York, May 2002, and a timetable was set for
global elimination by 2005. At the same time, the Network for Sustained
Elimination of Iodine Deficiency (the Network) was launched at a
side event during UNGASS by the Director General of WHO which
included contributions of high-level global leaders.
Many countries are now seeking acknowledgement of their USI
accomplishments and asking for external reviews to assist them in achiev-
ing USI. The Network will provide USI/IDD review through simplified
guidelines and in a cost-effective manner. Countries that have achieved
USI can request a desk assessment by providing relevant data and infor-
mation (see Annex 1) to the Network. For countries whose progress is
stalled and with substantial gains towards USI yet to be made, external
review missions might still be needed.
A6.2 Objectives
The following guidelines provide a framework for conducting the USI/
IDD country assessment. The main objectives of the review are:
• To help governments and program managers assess and verify the
country achievement towards their goals to sustain elimination of
iodine deficiency;
• To identify lessons learned and best practices of the country pro-
grams;
• To facilitate progress comparisons across regions by means of stand-
ardized tools/guidelines;
1
Adapted from: Country review guidelines. Network for sustained elimination of iodine
deficiency. New York, 2006 (41).
83
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Country report:
• The country report will be an important means of communicating the
country situation. It should include a short executive summary and
clear, concise summary on how the country plans to sustain the USI
achievements.
• It will be important to have the report (or at least the executive sum-
mary and country plans) available to distribute at the ‘stakeholder’/
partners consultation prior to submission to the Network.
• Please see Annex 1 for a suggested outline for the country report.
Representatives of Network member organizations could be involved in
the desk review, and if needed and resources are available, the Network
might have an external expert.
84
ANNEX 6. GUIDELINES TO ASSESS IDD NATIONAL PROGRAMMES
A report will be prepared by the National Coalition in coordination with government and
assistance from UNICEF, WHO and other Network member organizationsb in the country.
Network member organizationsb in the country and a local peer reviewer confirm/verify the
report/data and decision is made to organize either desk review or in country assessment.
In-country
assessment Desk review
Reports submitted to the Reports submitted to the
Secretary and the country requests Secretary and the country requests
for an in-country assessment. for a desk review.
The Secretary will share the reports Secretary will coordinate with
with Network Board members. Board members to draw on expertise
available within Network member
organizations (2–3 persons) and
If the Board agrees and resources or an external expert if needed and
are available, the Secretary will resources are available; and form a
coordinate with Board members, the virtual team for evaluation.
country and regional counterparts
for developing TORs and program
for the review mission. If resources Team reviews the report and
are available and needed, external submits recommendations to the
experts may be recruited. Network Secretary within
2 weeks.
a
Network Secretary can be contacted at Info@iodinenetwork.net.
b
Network member organizations in the country include UNICEF, WHO, WFP, Kiwanis International, Salt Institute,
EuSalt, China National Salt Industry Corporation, ICCIDD, MI, Emory U, US CDC, and GAIN.
c
UNICEF, PAMM, MI, ICCIDD and WHO. 1995. Assessing Country Progress in Universal Salt Iodization Programs:
Iodized Salt Program Assessment Tool (ISPAT). The Micronutrient Initiative, Ottawa, Canada.
85
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
86
ANNEX 6. GUIDELINES TO ASSESS IDD NATIONAL PROGRAMMES
Country assessment
a. The product
This section should include information on all aspects of salt production
or imports. It should focus on those aspects that pertain to the entire salt
industry and should provide details on those areas of industry responsi-
bility that can be improved and sustained. The discussion includes:
• Achievement of change in practices by food processing industries;
• Analysis of relationships between regulatory authorities and practices
and salt producers and practices;
• Analysis of utility and impact of product advertising on public
demand, use and understanding;
• Analysis of quality assurance at iodized salt production;
• Analysis on data of salt importation, production and iodization pro-
cess, distribution, major companies, small scale producers/salt farm-
ers, association of salt producers, prices of products and the market
situation;
• Analysis on availability and procurement of KIO3 ;
• A summary of salt situation (see Table 16);
• A summary of lessons learned;
• Key action plans related to the product might be elaborated.
b. The process
This section should include all elements necessary for the long-term con-
tinuation of the program. The focus should be on elements that are the
roles and responsibility of the national IDD program, or of other branch-
es of the government. This should include assessments of each element’s
strengths and weaknesses, with specific suggestions for improvement.
The discussion includes:
• Analysis of the political process and how that has been nurtured and
sustained, and with what measurable results;
• Analysis of the history of formation of a National Coalition to assure
achievement of USI and Sustained Iodine Nutrition and the current
practices and issues;
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ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
88
ANNEX 6. GUIDELINES TO ASSESS IDD NATIONAL PROGRAMMES
89
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Table 17 Continued
PROGRAMMATIC COUNTRY PROGRAM ACTION PLANS TO
INDICATORS SITUATIONS SUSTAIN USI
Conclusions
Conclusion on the country situation analysis and summary of agreed key
action plans to sustain ID elimination in the country.
90
ANNEX 7
List of Contributors
Bruno de Benoist
Micronutrient Malnutrition Unit (MNM)
Department of Nutrition for Health and Development
World Health Organization
20 Avenue Appia
CH-1211, Geneva 27
Switzerland
Tel: +41 22 791 3412
Fax: +41 22 791 4156
E-mail: debenoistb@who.int
Gerard N. Burrow
Chairman
International Council for Control of Iodine Deficiency Disorders
110 Deepwood Drive
Hamden, CT 06517
USA
E-mail: gburrow55@comcast.net
François Delange1
ICCIDD
Hyde Park Residence
Chaussée de Waterloo 965 D.O.3
B 1180 Brussels
Belgium
Tel: +32 (2) 660 04 08
Fax: +32 (2) 660 0408
E-mail: fdelange@ulb.ac.be
1
Deceased
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ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Jonathan Gorstein
University of Washington
School of Public Health and Community Medicine
H-688 Health Sciences Building
Box 357660
1959 NE Pacific St.
Seattle, WA 98195
United States of America
Tel: 206–616–8530
Fax: 206–543–3964
Email: gorstein@u.washington.edu
Robin Houston
Monitoring and evaluation for vitamin A
MOST Project
9455 Big Gulch Road
Bozeman, MT 59715
United States of America
Tel: +1 (406) 582 7959
Fax: +1 (406) 582 7959
E-mail: houstonrmh@yahoo.com
Pieter Jooste
Nutritional Intervention Research Unit
PO Box 19070
Tygerberg 7505
Cape Town
South Africa
Tel: +27 (0) 21 938-0370
Fax: +27 (0) 21 938-0321
E-mail: pieter.jooste@mrc.ac.za
Nuné Mangasaryan
Nutrition and Child Growth & Development
Programme Division
United Nations Children’s Fund (UNICEF)
3 United Nations Plaza, New York
NY 10017, USA
Tel: + 1 212 326 71 59
Fax: + 1 212 326 71 29
E-mail: nmangasaryan@unicef.org
92
ANNEX 7. LIST OF CONTRIBUTORS
Erin McLean
Micronutrient Malnutrition Unit (MNM)
Department of Nutrition for Health and Development
World Health Organization
20 Avenue Appia
CH-1211, Geneva 27
Switzerland
Tel: +41 22 791 3348
Fax: +41 22 791 4156
E-mail: mcleane@who.int
Eduardo Pretell
ICCIDD
Av. Paseo de la República 3691, Of. 401-A
San Isidro, Lima 27, Perú
E-mail: epretell@terra.com.pe
Tel: +(51-1) 421-0882
Fax: +(51-1) 421-3065
E-mail: epretell@terra.com.pe
Lisa Rogers
Micronutrient Malnutrition Unit (MNM)
Department of Nutrition for Health and Development
World Health Organization
20 Avenue Appia
CH-1211, Geneva 27
Switzerland
Tel: +41 22 791 1957
Fax: +41 22 791 4156
E-mail: rogersl@who.int
Kevin Sullivan
Department of Pediatrics, Epidemiology and International Health
Emory University
1518 Clifton Road, N.E.
Atlanta, GA 30322
United States of America
Tel.: +1 (404) 727 8714
Fax: +1 (404) 727 5369
E-mail: cdckms@sph.emory.edu
93
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Juliawati Untoro
Nutrition and Child Growth & Development
Programme Division
United Nations Children’s Fund (UNICEF)
3 United Nations Plaza, New York
NY 10017, USA
Tel: + 1 212 326 73 68
Fax: + 1 212 326 71 29
E-mail: juntoro@unicef.org
Michael Zimmermann
Laboratory of Human Nutrition
Swiss Federal Institute of Technology (ETH) Zürich
ETH Zentrum, Schmelzbergstrasse 7, LFV E19
CH-8092 Zürich
Switzerland
Tel: +41 44 632 8657
Fax: +41 44 632 1470
E-mail: michael.zimmermann@ilw.agrl.ethz.ch
94
References
95
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
96
REFERENCES
97
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
98
ASSESSMENT OF IODINE DEFICIENCY DISORDERS AND MONITORING THEIR ELIMINATION
Assessment of
iodine deficiency disorders and
monitoring their elimination
This document is intended primarily for managers of national program-
mes for the prevention and control of micronutrient malnutrition.
It sets out principles governing the use of surveillance indicators in imple-
menting interventions to prevent, control, and monitor iodine deficiency
disorders (IDD). It presents methods for monitoring iodine status and
determining urinary iodine and provides guidelines on the procedures for
monitoring salt iodine content, whether at the factory, importation site, or
household level. It also gives guidance on conducting surveys to assess
iodine status in populations.
Finally, indicators are presented for monitoring progress towards achie-
ving the goal of sustainable elimination of IDD as a significant public
health problem.