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Cervical speculum examination

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and taking a smear.
Title page
Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices
Slide method
Liquid-based cytology
Swabs Cusco
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Sims
Learning Outcomes
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Title page
Speculum examination

ƒ Able assemble the speculum


Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices
Slide method
correctly
ƒ Able to inspect vagina and cervix
Liquid-based cytology
Swabs
Removing the speculum

using a speculum
Ending the procedure
Summary
Further reading

ƒ Perform a cervical smear


Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Speculum examination
1. Designed for the inspection of the
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Speculum examination
Inspection of vagina
Inspection of cervix
cervix and vaginal walls.
Cervical smear
methods Bivalve (cusco) speculum is the instrument
devices
Slide method most commonly used to inspect the
Liquid-based cytology
Swabs
vagina
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
2. Access to the cervix and fornices
age
risk factors
for bacteriological swabs and
pathology – micro
pathology – macro
presentation
cervical smears.
investigation
Cervical Screening
Examination
ƒ GRIP
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Title page
Speculum examination
Inspection of vagina
Inspection of cervix
ƒ Greet, rapport, introduce and identify, explain
Cervical smear
methods
procedure
ƒ Tell the patient the procedure is not
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
painful, but may be a bit uncomfortable.
ƒ Make sure:
Ending the procedure
Summary
Further reading

ƒ not menstruating
Cervical Cancer
incidence
age
risk factors
pathology – micro
ƒ no spermicide, lubricant, sexual intercourse
pathology – macro
presentation
in the previous 48 hours.
investigation
Cervical Screening
Position
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Title page
Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
“Lie on the couch, put your heels together, draw them up to
your bottom and let your knees flop apart”
Speculum examination
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Speculum examination
ƒ Warm blades under a stream of tepid
Inspection of vagina
Inspection of cervix
water.
Cervical smear
methods
ƒ Wear gloves, prepare the slide/bottle
ƒ
devices
Slide method
Liquid-based cytology
Write name of the patient, date and time
Swabs
Removing the speculum
of taking the specimen with pencil not
Ending the procedure
Summary
pen if using slide
Further reading
Cervical Cancer
incidence
ƒ Hold the speculum with dominant hand,
age
risk factors
separate the labia and expose introitus
pathology – micro
pathology – macro
with non-dominant hand
presentation
investigation
ƒ Tell the patient you are about to
Cervical Screening
introduce the speculum and reassure
Speculum examination
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Speculum examination
ƒ Slide the closed blades obliquely over
Inspection of vagina
Inspection of cervix
the fingers into the introitus , introduce
Cervical smear
methods
the instrument into the vagina
devices
Slide method

ƒ While inserting the instrument rotate it to


Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
Summary
a clockwise direction until the anterior
Further reading
Cervical Cancer
and posterior blades run along the
incidence
age
anterior and posterior vaginal walls with
risk factors
pathology – micro
the handles pointing towards the anus or
pathology – macro
presentation clitoris.
investigation
Cervical Screening
Speculum examination
ƒ Maintain a downward pressure, on
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Speculum examination

the speculum and press on the


Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices thumb piece to hinge the blades
Slide method
Liquid-based cytology
Swabs
open to expose the vaginal vault
and cervix.
Removing the speculum
Ending the procedure
Summary

ƒ Adjust the light source to illuminate


Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
the vagina.
pathology – macro
presentation
investigation
ƒ Inspect vagina and cervix.
Cervical Screening
Inspection of the vagina
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Speculum examination
Discharge:
Inspection of vagina
Inspection of cervix
ƒ Normal – clear / milky, odourless
Cervical smear
methods
ƒ Increased during ovulation, pregnancy, breast-
devices feeding, sexual arousal
ƒ Cottage cheese / curd-like: candida (assoc.
Slide method
Liquid-based cytology
Swabs
Removing the speculum with pain, itching, swelling and erythema of
Ending the procedure
Summary
the vulva)
ƒ Frothy, fishy odour: Trichomoniasis
Further reading
Cervical Cancer

ƒ Asymptomatic discharge: gonorrhoea


incidence
age
risk factors
pathology – micro
pathology – macro Ulcers
presentation
investigation
Cervical Screening
Foreign body
Inspection of the cervix
ƒ Cervix normally points posteriorly
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Speculum examination
Inspection of vagina
Inspection of cervix
and inferiorly
Cervical smear
methods
devices
Slide method
Liquid-based cytology
Swabs
Look for:
ƒ Discharge
Removing the speculum
Ending the procedure
Summary

ƒ Cysts
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
ƒ Polyps
pathology – macro
presentation
investigation
ƒ Ulceration or fungating growth
Cervical Screening
Normal with
Normal
ectropion

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Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening

cervicitis cancer
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Title page
Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Nabothian cyst – mucus filled cervical cyst due to blockage of
a mucus gland
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Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening

Cervical polyp (appears to be descending through the os)


Cervical smear
ƒ Cervical smear performed after
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Speculum examination

inspecting the cervix.


Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices The aim is to
Slide method
sample cells
Liquid-based cytology
Swabs from the
Removing the speculum transformation
Ending the procedure
Summary zone (where the
Further reading epithelium
Cervical Cancer
incidence changes from
age columnar to
risk factors
pathology – micro squamous)
pathology – macro
presentation
investigation
Cervical Screening
Cervical smear - methods
ƒ Newer method = LBC ( Liquid–based
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Speculum examination
Inspection of vagina
Inspection of cervix
cytology )
ƒ
Cervical smear
methods Use cervex brush (see next slide)
devices
Slide method
Liquid-based cytology
ƒ Dip brush into bottle of liquid fixative
Swabs
Removing the speculum ƒ Fewer inadequate samples. Being rolled out
Ending the procedure
Summary across the UK
Further reading
Cervical Cancer
incidence
age
risk factors ƒ Older method = slides
pathology – micro
pathology – macro
presentation
ƒ Use Aylesbury spatula (see next slide)
investigation
Cervical Screening
ƒ Spray or dip in fixative
Cervical smear - devices
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Speculum examination
LBC
Inspection of vagina
Inspection of cervix
Cervical smear
ƒ A cervex brush is
methods
devices
commonly used
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure Slide-based
Summary
Further reading
Cervical Cancer
ƒ A wooden spatula
incidence
age is used with a bifid
risk factors
pathology – micro
pathology – macro
end (Aylesbury
presentation
investigation
spatula) .
Cervical Screening
Liquid Based Cytology method
ƒ Use a cervex brush
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Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ Place the tip of the brush in the os


methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
ƒ Twirl 10 times
Summary
Further reading
Cervical Cancer
ƒ Place brush in fixative bottle and dip
incidence
age / stir / squish vigorously to wash the
risk factors
pathology – micro
pathology – macro
cells off the brush into the bottle
presentation
investigation
Cervical Screening
Slide method
ƒ The bifid end is used to harvest the
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Speculum examination

cervical cells.
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ Introduce the spatula through the


methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
speculum and position the bifid end
Ending the procedure
Summary
at the os.
ƒ Rotate through 360°
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
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Title page
Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
age
risk factors
The desquamating cells are
pathology – micro
pathology – macro
presentation
collected by rotating the spatula
investigation
Cervical Screening
around the circumference of the
os and the lips of the cervix.
Slide method
ƒ Send sample to the lab
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Title page
Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ Spread the cervical material onto the


methods
devices
Slide method
Liquid-based cytology
Swabs
labelled glass slide by stroking each
Removing the speculum
Ending the procedure side of the bifid end of the spatula
Summary
Further reading
Cervical Cancer
along the glass
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Slide method
ƒ The cervical cells and some mucus
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Speculum examination

should cling to the glass.


Inspection of vagina
Inspection of cervix
Cervical smear

ƒ Immediately spray the slides with


methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
fixative or fix them by immersion in
Ending the procedure
Summary
95% alcohol
ƒ Do not allow the sample to dry.
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation Some examples of cells processed with the Papanicolau stain
investigation
Cervical Screening
Swabs
ƒ If any ulcers, take swab from base of
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Title page
Speculum examination
Inspection of vagina
Inspection of cervix
ulcer.
Cervical smear
methods
devices

ƒ If infection suspected, take swab from


Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
cervix or high vaginal.
Summary
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Removing the speculum
ƒ Undo the thumb screw
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Title page
Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
ƒ Simultaneously withdraw the speculum
methods
devices
and rotate the open blades to ensure that
Slide method
Liquid-based cytology
the anterior and posterior walls of vagina
Swabs
Removing the speculum can be inspected.
Ending the procedure
Summary
Further reading ƒ Near the introitus, allow the blades to
Cervical Cancer
incidence
age
close
risk factors
pathology – micro ƒ Taking care not to pinch the labia or any
pathology – macro
presentation
investigation
hairs while withdrawing the speculum.
Cervical Screening
Ending the procedure
ƒ Tell the patient that you have
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Title page
Speculum examination

finished , give a towel to the patient


Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices to wipe herself.
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
age
ƒ Send samples to lab
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Summary
Jump menu ƒ Necessary equipment ƒ Wash hands, don gloves
Title page ƒ Cusco’
Cusco’s speculum ƒ Label bottle and open the lid
Speculum examination ƒ Cervix brush ƒ Assemble speculum
ƒ Specimen bottle
ƒ
Inspection of vagina
If not in warmer, warm under tap
Inspection of cervix ƒ Microbiology swabs
Cervical smear ƒ Water based lubricant ƒ Use fingers to keep blades shut
methods ƒ Tissues ƒ Warn patient
devices ƒ Introduce yourself ƒ Separate labia
Slide method
ƒ Identify patient (full name and DOB) ƒ Insert with rotating motion
Liquid-based cytology
ƒ Procedure – ƒ Avoid touching clitoris
Swabs
Removing the speculum
ƒ Explain what you are going to do and ƒ Under direct vision open the blades
what she may experience and angle the speculum accordingly
Ending the procedure
ƒ Permission so that cervix comes into view
Summary
Further reading
ƒ Obtain informed consent ƒ Inspect cervix and lateral vaginal
ƒ Privacy walls
ƒ
Cervical Cancer
incidence ƒ Position Insert tip of cervex brush into os
age ƒ Heels together, draw heels towards ƒ Rotate 10X, sample whole cervix
risk factors
bottom, let knees flop apart ƒ Remove brush and dip vigorously into
pathology – micro
ƒ Light bottle
pathology – macro ƒ Exposure ƒ Remove the speculum gently, rotate
presentation ƒ Remove clothing from the waist to inspect anterior and posterior
investigation
down, cover with blanket vaginal walls
Cervical Screening ƒ Let the blades close carefully
ƒ Offer patient some tissues to wipe
herself, cover her with blanket and
leave the curtained area
Further Reading
ƒ Why screen regularly with smear
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Title page
Speculum examination

test
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ What is CIN ?
methods
devices
Slide method

ƒ What are the stages ?


Liquid-based cytology
Swabs
Removing the speculum

ƒ How do we treat ?
Ending the procedure
Summary
Further reading

ƒ How does CIN differ from invasive


Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
cancer
presentation
investigation
Cervical Screening
Further reading
ƒ http://www.cancerscreening.nhs.uk/
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Title page
Speculum examination

cervical/publications/cervicalpocket2
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices 004.pdf
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
Summary
Further reading
ƒ http://www.gfmer.ch/Books/Cervical
Cervical Cancer
incidence _cancer_modules/Aided_visual_ins
age
risk factors
pathology – micro
pection_atlas.htm
pathology – macro
presentation
investigation
Cervical Screening
Cervical Screening
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Title page General screening criteria
Speculum examination
Inspection of vagina

ƒ The disease should be responsible for


Inspection of cervix
Cervical smear
methods
devices significant morbidity or mortality in the target
Slide method
Liquid-based cytology population
Swabs
Removing the speculum ƒ The natural history of the disease should be
Ending the procedure
Summary
known
Further reading
Cervical Cancer ƒ Intervention at an early stage should be
incidence
age
effective in preventing the condition
risk factors
pathology – micro ƒ A screening programme should be cost effective
ƒ
pathology – macro
presentation Treatment should cause low morbidity
investigation
Cervical Screening
Cervical Screening
ƒ Almost four million women are
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Title page
Speculum examination
Inspection of vagina
Inspection of cervix
screened each year
Cervical smear
methods
devices

ƒ 42% fall in incidence of cervical


Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure cancer between 1988 and 1997
Summary
Further reading
Cervical Cancer
attributable to screening
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
ƒ Mortality rates in 2000 were 60%
investigation
Cervical Screening
lower than 30 years earlier
Cervical Screening
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Title page
ƒ The screening programme in England and
Speculum examination
Inspection of vagina
Wales invites all women between 25 and 64
Inspection of cervix years for three- or five-yearly screening.
Cervical smear
methods
devices
Slide method ƒ It is recommended that women 25 to 49 are
Liquid-based cytology
Swabs
screened three-yearly and 50 to 64 five yearly.
Removing the speculum
Ending the procedure
Summary
Further reading
ƒ Women over 65 are only called if they have not
Cervical Cancer had a smear since age 50, or have had a recent
incidence
age
abnormality.
risk factors
pathology – micro
pathology – macro ƒ In Scotland the programme is slightly different, with women
presentation between the ages of 20 and 60 being invited for screening every
investigation
Cervical Screening
three years.
Cervical Intraepithelial
Neoplasia
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ƒ CIN I- Dysplasia in the basal 1/3


Title page
Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ CIN 2- Dysplasia extending to middles


methods
devices
Slide method
Liquid-based cytology
Swabs
1/3
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
ƒ CIN 3- Dysplasia involving the whole
incidence
age
epithelium but the basal membrane is
risk factors
pathology – micro intact.
pathology – macro
presentation
investigation Dysplasia
Cervical Screening
ƒ basal hyperplasia - multi-layering of basal cells
ƒ nuclear pleiomorphism
ƒ increased mitotic activity
Cervical Cancer
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Title page
Speculum examination
Incidence
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices ƒ 2400 new cases per year in England and
Slide method
Liquid-based cytology
Swabs
Wales
Removing the speculum
Ending the procedure
Summary
Further reading
Cervical Cancer
ƒ <1000 deaths annually
incidence
age
risk factors

ƒ ½ of women who present with late stage


pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
disease have never had a smear
Cervical Cancer
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Title page
Speculum examination
Age
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ Rare under 20yrs


methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
Ending the procedure
ƒ Peak 45-55yrs
Summary
Further reading
Cervical Cancer
ƒ Peaks earlier in pts from lower
incidence
age socioeconomic groups
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Cervical Cancer
Jump menu
Title page Risk factors
ƒ Age
Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
methods
ƒ Lower social class
devices
Slide method
ƒ HPV infection (types 16 and 18 mainly)
Liquid-based cytology
Swabs ƒ Large numbers of sexual partners
ƒ Partner with many previous partners
Removing the speculum
Ending the procedure

ƒ Smoking
Summary
Further reading
Cervical Cancer
incidence
age
ƒ Immunosuppression
risk factors
pathology – micro
ƒ Early age of 1st intercourse and 1st pregnancy
pathology – macro
presentation ƒ Abnormal smear
investigation
Cervical Screening ƒ Oral contraceptive use rather than barrier
Cervical Cancer
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Title page
Speculum examination
Pathology - micro
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ 80% squamous carcinoma of


methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
transformation zone
Ending the procedure
Summary
Further reading

ƒ Rest are adenocarcinoma


Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Cervical Cancer
Jump menu
Title page
Speculum examination
Pathology – macro
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ Ulceration or mass on cervix that


methods
devices
Slide method
Liquid-based cytology
Swabs
Removing the speculum
bleeds easily
Ending the procedure
Summary
Further reading
Cervical Cancer
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening
Cervical Cancer
Jump menu
Title page
Speculum examination
Common Presentations
Inspection of vagina
Inspection of cervix
Cervical smear
methods
devices
Slide method
ƒ During routine smear
Liquid-based cytology
Swabs
Removing the speculum
ƒ Postcoital bleeding
Ending the procedure
Summary ƒ Intermenstrual bleeding
ƒ Postmenopausal bleeding
Further reading
Cervical Cancer
incidence

ƒ Offensive vaginal discharge


age
risk factors
pathology – micro

ƒ Pain
pathology – macro
presentation
investigation
Cervical Screening
Cervical Cancer
Jump menu
Title page Investigations
Speculum examination
Inspection of vagina

ƒ Urgent referral for colposcopy


Inspection of cervix
Cervical smear
methods
devices
Slide method
ƒ Direct visualisation
Liquid-based cytology
Swabs
ƒ Green light – abnormal blood vessels
Removing the speculum
Ending the procedure
ƒ Acetic acid – causes coagulation of nuclear
Summary proteins, hence areas of high nuclear density
Further reading
Cervical Cancer
appear white
incidence
age
ƒ Iodine – Schiller’s test – abnormal immature
risk factors
pathology – micro
epithelium doesn’t contain glycogen and
pathology – macro therefore doesn’t stain with iodine
presentation
investigation
Cervical Screening
Other test
ƒ FBC
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Speculum examination
Inspection of vagina
Inspection of cervix
Cervical smear
ƒ U&E
methods
devices
Slide method
ƒ LFT
Liquid-based cytology
Swabs
Removing the speculum
ƒ Serum Ca
Ending the procedure
Summary ƒ Chest X-ray
ƒ CT scan
Further reading
Cervical Cancer
incidence

ƒ In advanced disease consider


age
risk factors
pathology – micro

cystoscopy / sigmoidoscopy
pathology – macro
presentation
investigation
Cervical Screening
Treatment
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Title page
Speculum examination
Depend on the stage of the disease
Inspection of vagina
Inspection of cervix
Cervical smear

ƒ Cone biopsy
methods
devices
Slide method Radical
Liquid-based cytology
Swabs
Removing the speculum
Hysterectomy
Ending the procedure
Summary
Further reading
Cervical Cancer
ƒ Radiotherapy Stage I-IV
ƒ Chemotherapy for some cases
incidence
age
risk factors
pathology – micro
pathology – macro
presentation
investigation
Cervical Screening

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