Nama Maba : Grace Firsty Putri F

Nama Garda: Bacteriology

MORAXELLA CATARRHALIS

Moraxella catarrhalis is a major cause of infectious exacerbations of

chronic obstructive lung disease (COPD) and may also contribute to the
pathogenesis of COPD. Little is known about M. catarrhalis-bronchial epithelium
interaction. We investigated activation of M. catarrhalis infected bronchial
epithelial cells and characterized the signal transduction pathways. Moreover, we
tested the hypothesis that the M. catarrhalis-induced cytokine expression is
regulated by acetylation of histone residues and controlled by histone deacetylase
activity (HDAC). We demonstrated that M. catarrhalis induced a strong time-
and dose-dependent inflammatory response in the bronchial epithelial cell line
(BEAS-2B), characterized by the release of IL-8 and GM-CSF. For this cytokine
liberation activation of the ERK and p38 mitogen-activated protein (MAP)
kinases and transcription factor NF-κB was required. Furthermore, M.
catarrhalis-infected bronchial epithelial cells showed an enhanced acetylation of
histone H3 and H4 globally and at the promoter of the il8 gene. Preventing
histone deacetylation by the histone deacetylase inhibitor trichostatin A
augmented the M. catarrhalis-induced IL-8 response. After exposure to M.
catarrhalis, we found a decrease in global histone deacetylase expression and
activity

The surface of the airway epithelium represents a battleground in which

the host intercepts signals from pathogens and activates epithelial defenses to
combat infection. Wound repair is an essential function of the airway epithelium
in response to injury in chronic airway diseases, and inhaled pathogens such as
Pseudomonas bacteria are implicated in the pathobiology of several of these
diseases. Because epidermal growth factor receptor (EGFR) activation stimulates
wound repair and because LPS activates EGFR, we hypothesized that LPS
accelerates wound repair via a surface signaling cascade that causes EGFR
phosphorylation. In scrape wounds of NCI-H292 human airway epithelial cells,
high concentrations of LPS were toxic and decreased wound repair. However,
lower concentrations of LPS accelerated wound repairMoraxella catarrhalis, an
important pathogen in the human respiratory tract, causes otitis media and lower
respiratory tract infections. M. catarrhalis outer membrane protein CD
(OMPCD) is a major heat-modifiable OMP with demonstrable potential as a
vaccine candidate. The gene encoding OMPCD of M. catarrhalis strains was
subjected to nucleotide sequence analysis and then inactivated by insertional
 Moraxella catarrhalis, patogen penting pada saluran pernapasan manusia,
menyebabkan otitis media dan infeksi saluran pernapasan bawah. M. catarrhalis
luar CD protein membran (OMPCD) adalah OMP panas dimodifikasi besar
dengan potens i dibuktikan sebagai kandidat vaksin. Gen encoding OMPCD M.
catarrhalis strain menjadi sasaran nukleotida analisis urutan dan kemudian aktif
oleh mutagenesis insersional.
Daftar Pusaka

H. Slevogt, et al. M. catarrhalis induces inflammatory response of

bronchial epithelial cells via MAPK and NF-kappaB activation and
histone deacetylase activity reduction. Am. J. Physiol. Lung Cell. Mol.
Physiol., 290 (5) (2006), pp. L818–L826

(http://www.sciencedirect.com/science/article/pii/S0006291X1401176
0, diakses pada 26 Agustus 2016 )
J.L. Koff, et al. Pseudomonas lipopolysaccharide accelerates wound
repair via activation of a novel epithelial cell signaling cascade. J.
Immunol., 177 (12) (2006), pp. 8693–8700
H. Slevogt, et al. M. catarrhalis is internalized in respiratory epithelial
cells by a trigger-like mechanism and initiates a TLR2- and partly
NOD1-dependent inflammatory immune response Cell. Microbiol., 9
(3) (2007), pp. 694–707
(http://www.sciencedirect.com/science/article/pii/S0006291X1401176
0, diakses pada 26 Agustus 2016 )
Current progress with Moraxella catarrhalis antigens as vaccine
candidates. Expert Review of Vaccines 8:1, 77-90. Online publication
date: 1-Jan-2009.
(http://www.atsjournals.org/doi/full/10.1164/rccm.200412-
1747OC#.V8A_ca0quKE ,
diakses pada 26 Agustus 2016 )
Role of Moraxella catarrhalis outer membrane protein CD in bacterial
cell morphology and autoaggregation. Research in Microbiology 164:3,
236-243. Online publication date: 1-Apr-2013.
(http://www.atsjournals.org/doi/full/10.1164/rccm.200412-
1747OC#.V8A_ca0quKE, diakses pada 26 Agustus 2016 )