ABSTRACT

CONJUGATED DIENE FORMATION IN ERYTHROCYTES

INDUCED BY CEFTAZIDIME PHOTOSENSITIZER In Vitro

Muhammad Rifqi Alfiannoor

Photosensitiser is the causative agent of photosensitivity. One of them is

ceftazidime which is a group of antibiotics from third generation cephalosporin
group. The ultraviolet (UV) induced ceftazidime can cause the transfer of energy
and electron. Both of these mechanisms will damage one of the lipids present in
erythrocytes. The damage causes lipid peroxidation. One product of lipid
peroxidation is conjugated diene. The concentration of conjugated diene is one of
the parameters of oxidative damage of erythrocyte cell membranes and counted
with spectrophotometry. This study aims to determine the effect of UV-induced
ceftazidime on the formation of conjugated diene. This study was a pure
experimental study with one control group and five treatment groups. The control
group (P0) contained erythrocyte + phosphate buffer pH 6.8%, while the
treatment group 1 (P1) contains erythrocyte + phosphate buffer pH 6.8% + UV,
group 2 (P2) contains erythrocyte + ceftazidime 10% + UV, group 3 (P3)
contains erythrocyte + ceftazidime 20% + UV, group 4 (P4) contains erythrocyte
+ ceftazidime 30% + UV, and group 5 (P5) contains erythrocyte + ceftazidime
40% + UV. The result of Kruskal-Wallis test in this study showed that there were
significant differences in various groups (P < 0.05). Based on these statistical
tests it was concluded that ceftazidime may influence the formation of conjugated
diene in erythrocytes induced by UV.

Keywords: Ultraviolet, ceftazidime, photosensitiser, lipid peroxidation,

conjugated diene.

Universitas Lambung Mangkurat