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  • Kromatogram Optimasi Kolom Pelatihan Lcmsms Ot 2017

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    Adhe Sri Marjuki
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    The document contains three chromatograms showing intensity over time from liquid chromatography-mass spectrometry analysis. The chromatograms show peaks between 7 and 8 minutes, likely indicating detection of the target analyte. Accompanying information provides details on the analytical method used, including changing pump concentrations over time and different column types used.

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    The document contains three chromatograms showing intensity over time from liquid chromatography-mass spectrometry analysis. The chromatograms show peaks between 7 and 8 minutes, likely indicating detection of the target analyte. Accompanying information provides details on the analytical method used, including changing pump concentrations over time and different column types used.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    9 views5 pages

    Kromatogram Optimasi Kolom Pelatihan Lcmsms Ot 2017

    Uploaded by

    Adhe Sri Marjuki
    The document contains three chromatograms showing intensity over time from liquid chromatography-mass spectrometry analysis. The chromatograms show peaks between 7 and 8 minutes, likely indicating detection of the target analyte. Accompanying information provides details on the analytical method used, including changing pump concentrations over time and different column types used.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 5

    XIC of +MRM (36 pairs): 491.200/100.200 Da ID: tiosildenafil1 from Sample 11 (Baku mix 12) of DataSET1.

    wiff (Turbo Spray) Max. 0.0 cps.

    1.05e5

    1.00e5

    9.50e4

    9.00e4

    8.50e4

    8.00e4

    7.50e4

    7.00e4

    6.50e4
    I n t e n s it y , c p s

    6.00e4

    5.50e4

    5.00e4

    4.50e4

    4.00e4

    3.50e4

    3.00e4

    2.50e4

    2.00e4

    1.50e4

    1.00e4

    5000.00

    0.00
    0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
    Time, min

    kromatogram kolom Agilent Eclipse plus C18 21x50mm 5um

    ============
    Time Module Events Parameter
    0.01 Pumps Pump B Conc. 10
    2.00 Pumps Pump B Conc. 30
    4.00 Pumps Pump B Conc. 90
    4.25 Pumps Pump B Conc. 10
    5.50 Pumps Pump B Conc. 10
    5.51 System Controller Stop
    XIC of +MRM (36 pairs): 491.200/100.200 Da ID: tiosildenafil1 from Sample 13 (Baku 500 ppb) of Seri Baku.wiff (Turbo Spray) Max. 2.4e4 cps.

    1.29e5
    1.25e5
    1.20e5
    1.15e5
    1.10e5
    1.05e5
    1.00e5
    9.50e4
    9.00e4
    8.50e4
    8.00e4
    I n t e n s it y , c p s

    7.50e4
    7.00e4
    6.50e4
    6.00e4
    5.50e4
    5.00e4
    4.50e4
    4.00e4
    3.50e4
    3.00e4
    6.65
    2.50e4
    2.00e4
    1.50e4
    1.00e4
    5000.00
    0.00
    0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5
    Time, min

    kromatogram kolom Agilent Eclipse plus C18 21x50mm 5um


    ============
    Time Module Events Parameter
    0.01 Pumps Pump B Conc. 10
    2.00 Pumps Pump B Conc. 30
    8.00 Pumps Pump B Conc. 90
    8.20 Pumps Pump B Conc. 10
    9.20 Pumps Pump B Conc. 10
    10.00 System Controller Stop
    XIC of +MRM (36 pairs): 491.200/100.200 Da ID: tiosildenafil1 from Sample 1 (Baku 500 ppb) of Seri Baku 22.wiff (Turbo Spray) Max. 2.2e4 cps.

    1.8e5

    1.7e5

    1.6e5

    1.5e5

    1.4e5

    1.3e5

    1.2e5

    1.1e5
    I n t e n s it y , c p s

    1.0e5

    9.0e4

    8.0e4

    7.0e4

    6.0e4

    5.0e4

    4.0e4

    3.0e4
    7.30
    2.0e4

    1.0e4

    0.0
    0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5
    Time, min

    Kolom Phenomenex Kinetex 2.6um C18 50mmx3.0mm

    ============
    Time Module Events Parameter
    0.01 Pumps Pump B Conc. 10
    2.00 Pumps Pump B Conc. 30
    8.00 Pumps Pump B Conc. 90
    8.20 Pumps Pump B Conc. 10
    9.20 Pumps Pump B Conc. 10
    10.00 System Controller Stop
    XIC of +MRM (36 pairs): 521.300/503.000 Da ID: hidroksitiohomo3 from Sample 40 (LOD1 20X) of Seri Baku 22.wiff (Turbo Spray) Max. 200.0 cps.

    2200

    2100
    S/N = 3.5
    2000

    1900 Ymax=5.0e+1 cps Ymin=0.0e+0 cps


    1800

    1700 Peak Int.(Subt.)=1.8e+2

    1600

    1500

    1400
    I n t e n s it y , c p s

    1300

    1200

    1100

    1000

    900

    800

    700

    600

    500

    400

    300
    7.22
    200

    100
    ! - Noise - !
    0
    0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5
    Time, min

    Hidroksitiohomo

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