2.
2 Isolation of microbial DNA
blackPREP Tick DNA Kit
ƒƒ Optimized for extracting pathogen DNA from ticks
ƒƒ Includes application-specific Lysis Tubes with homogeniza-
tion beads
ƒƒ When combined with rapidSTRIPE assays, the kit provides
a highly sensitive means of detecting tick-borne pathogens
ƒƒ Based on well-established Spin Filter technology
ƒƒ All black Spin Filter available in colourless
Product description
The blackPREP Tick DNA Kit has been adapted specifically for isolat-
ing DNA from ticks. The kit contains Lysis Tubes with optimized
beads and can be used in combination with the SpeedMill
(Analytik Jena) or other homogenizers as an efficient means of me-
chanically disruption of ticks without degrading the DNA. The steps
that follow lysis (binding and washing the DNA; final elution) guar-
antee highly efficient DNA purification. Analytik Jena´s rapidSTRIPE
assays can then be used in later applications as fast, uncomplicated
tools for detecting tick-borne pathogens. These ready-to-use assays
can be used as highly specific tools for detecting Borrelia, Rickettsia,
Anaplasma and Babesia, among other pathogens.
Procedure
3 variety of different ticks and/or tick species. The nucleic acids were
1 2
1. Homogenize and lyse the tick
2. Bind the DNA to the Spin Filter membrane
3. Wash the bound DNA
4. Elute the DNA
Product specifications
Starting material:
Ticks
Extraction time:
Less than 1 hour (including lysis)
Average purity (A 260:A 280):
1.7 – 2.0
2.2
Kit components
Lysis Tube, Lysis Solution, Proteinase K, Binding Solution, Precipi-
tation Buffer, Washing Solutions, Prefilter (colourless), Spin Filter
(black), Receiver Tubes, Elution Buffer, user manual
2 Manual nucleic acid isolation/Enrichment
Storage conditions and stability
The blackPREP Tick DNA Kit will remain stable for at least 6 months
if stored in a dry place at room temperature (14 °C to 25 °C). The
recommended storage temperature for lyophilized Proteinase K
is 4 °C. Once the Proteinase K has been solubilized, it should be
stored in aliquots at –20 °C, because repeated freezing and thawing
will significantly reduce its activity.
Sample application
The blackPREP Tick DNA Kit was used to isolate the DNA from a
then introduced into a tick-specific amplification reaction. The PCR
products were visualized on an agarose gel as the final step.
Lane 1: DNA ladder
Lane 2 – 8: Tick-specific PCR products
Lane 9: Negative control
Other products for Tick Born Diseases
innuAMP Tick DNA Test.................................................................................168
rapidSTRIPE Rickettsia Assay....................................................................... 219
rapidSTRIPE Borrelia Assay.......................................................................... 220
rapidSTRIPE Anaplasma Assay................................................................... 222
rapidSTRIPE Babesia Assay......................................................................... 223
Order information
Order number Quantity
845-BP-3100010 10 reactions
845-BP-3100025 25 reactions
845-BP-3100050 50 reactions
844-MA205-2 Laboratory Notebook
49