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  • Methodology 1) Preparation of Sample Dilution

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    ammar muzafar
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    1) E. coli samples were diluted by factors of 10-3, 10-4, and 10-5 in sterile distilled water and aliquoted into specimen bottles. 2) For the pour plate method, 1 mL of each diluted sample was added to petri dishes containing molten nutrient agar, which was then swirled to coat the plates before solidifying. 3) For the spread plate method, 0.1 mL of each diluted sample was spread evenly over the surface of agar plates using an L-shaped rod. The plates from both methods were incubated for 24 hours.

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    Methodology

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    1) E. coli samples were diluted by factors of 10-3, 10-4, and 10-5 in sterile distilled water and aliquoted into specimen bottles. 2) For the pour plate method, 1 mL of each diluted sample was added to petri dishes containing molten nutrient agar, which was then swirled to coat the plates before solidifying. 3) For the spread plate method, 0.1 mL of each diluted sample was spread evenly over the surface of agar plates using an L-shaped rod. The plates from both methods were incubated for 24 hours.

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    9 views3 pages

    Methodology 1) Preparation of Sample Dilution

    Uploaded by

    ammar muzafar
    1) E. coli samples were diluted by factors of 10-3, 10-4, and 10-5 in sterile distilled water and aliquoted into specimen bottles. 2) For the pour plate method, 1 mL of each diluted sample was added to petri dishes containing molten nutrient agar, which was then swirled to coat the plates before solidifying. 3) For the spread plate method, 0.1 mL of each diluted sample was spread evenly over the surface of agar plates using an L-shaped rod. The plates from both methods were incubated for 24 hours.

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    of 3

    METHODOLOGY

    1) Preparation of sample dilution

    1 mL of the mixture from There would be a total of 6


    1 mL of E-Coli is the first bottle in transferred specimen bottles, in this
    tranferred into a specimen
    into another specimen bottle experiment, only the
    bottle containing 9ml to further dilute it and the dilution factor of 10-3 , 10-
    sterile distilled water 4, 10-5 is used.
    process is repeated
    2) Pour Plate Method

    10 ml of molten nutrient agar The agar is allowed to dry


    1 mL of mixture from the is put into the plate. After the
    specimen bottles is and then the petri dish is
    molten agar is added, it is then wrap with parafilm. It is then
    transferred into the plate swirl around to ensure proper left to incubate for 24 hours.
    coverage of the plate
    3) Spread Plate Method

    0.1 mL of mixture from the By using an L shaped glass


    specimen bottles is rod, the mixture is evenly The petri dish is wrap with
    transferred into sterile petri distributed to ensure proper parafilm. It is then left to
    coverage on the inner surface incubate for 24 hours.
    dish containing agar
    of the petri dish

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