Sample Name Component Name Enzyme Concentration Enzyme Activity Units

50% Codeine 1 0μL 0U

CO Codeine 1 0μL 0U
3x Codeine 1 0μL 0U
10x Codeine 1 0μL 0U
15x Codeine 1 0μL 0U
25x Codeine 1 0μL 0U
50x Codeine 1 0μL 0U
LCU Codeine 1 0μL 0U
MCU Codeine 1 0μL 0U
HCU Codeine 1 0μL 0U
2500ng/mL Codeine 2.5μL B-Gluc Codeine 1 2.5μL 50U
2500ng/mL Codeine 2.5μL B-Gluc Codeine 1 2.5μL 50U
2500ng/mL Codeine 2.5μL B-Gluc Codeine 1 2.5μL 50U
2500ng/mL Codeine 5μL B-Gluc Codeine 1 5μL 100U
2500ng/mL Codeine 5μL B-Gluc Codeine 1 5μL 100U
2500ng/mL Codeine 5μL B-Gluc Codeine 1 5μL 100U
2500ng/mL Codeine 10μL B-Gluc Codeine 1 10μL 200U
2500ng/mL Codeine 10μL B-Gluc Codeine 1 10μL 200U
2500ng/mL Codeine 10μL B-Gluc Codeine 1 10μL 200U
2500ng/mL Codeine 15μL B-Gluc Codeine 1 15μL 300U
2500ng/mL Codeine 15μL B-Gluc Codeine 1 15μL 300U
2500ng/mL Codeine 15μL B-Gluc Codeine 1 15μL 300U
2500ng/mL Codeine 20μL B-Gluc Codeine 1 20μL 400U
2500ng/mL Codeine 20μL B-Gluc Codeine 1 20μL 400U
2500ng/mL Codeine 20μL B-Gluc Codeine 1 20μL 400U
Actual Codeine Concentration Calculated Codeine Concentration Percent Recovery
25 24.58 98%
50 50.15 100%
150 153.35 102%
500 484.82 97%
750 776.72 104%
1250 1230.44 98%
2500 2505.15 100%
30 32 107%
50 35.22 70%
150 154.7 103%
2500 436.03 17%
2500 459.4 18%
2500 422.44 17%
2500 551.18 22%
2500 531.47 21%
2500 514.56 21%
2500 789.26 32%
2500 837.81 34%
2500 865.65 35%
2500 1182.91 47%
2500 1161.73 46%
2500 1174.08 47%
2500 1242.1 50%
2500 1257.11 50%
2500 1214.55 49%
 IMCSzyme Activity Units Trials
60%

50%

Percent Recovery 40%

Average % Recovery
30%

18% 20%

10%

21% 0%
50U 100U 200U 300U 400U
GUS Activity Units (U)

33%

47%

50%