Notes on Philippine Important Amoeba Spp. Part 1 (Pseudopodia spp.

)
Cyst Tropohozoite

Cystic No. of Chromatoidal Chromatoidal

Size/Shape Cytoplasm Karyosome Size/Shape Cytoplasm Endoplasm Nucleus Karyosome
Wall Nucleus Bars Bars

Nucleus
membrane is
Vacuoles
Rounded Grayish violet lined by fine,
5- Smooth, 10-60 bluish black Single, spherical
Entamoeba histolica 1-4 ends with Central with clear regularly Small, center
20micra/spherical definite micra/irr with nucleus;
glycogen demarcation arranged
ingested rbc
chromatin
granules
15-50micra,
Splintered Highly Spherical with
condensed, Grayish violet; Irregular
10- Smooth, ends with vacuolated thick nucleur
Entamoeba coli 1-8 Eccentric thickened, there is no clear chromatin Large eccentric
33micra/spherical definite glycogen containing membrane lined
rounded demarcation granules
mass bacteria with course
mass
1-4 Deeply Vacuole
Smooth, Grayish 6- Small/spherical Large irregular
Endolimax nana 6-10micra/ova darkly stained Grayish violet may contain
definite granular 15micra/irr largenoues centric/eccentric
stained curved rods bacteria
With clear
demarcation of
ecto and endo, With
5-
Entamoeba gingivalis X X X X X X same bacteria and Single/spherical Center
35micra/irr
pseudopodia rare rbc
direction of E.
histolytica
Violet gray
Vacuoles
granular Absent or
Smooth, 1 or 6- Granular, may contain Large
Iodamoeba butschlii 6-15micra/irr with large with stained Single/spherical
definite rarely 2 25micra/irr vacuolated bacteria and central/eccentric
vacuole small rods
yeast cells
unstained
With 4-8
3- Granular With chromatin
Dientamoeba fragilis X X X X X X chromatin 2
22micra/irr vacuolated granules
granules
 Notes on Philippine Important Amoeba Spp. Part 3 (Malarian spp.)
No. of
RBC Stage Size of RBC Dots Trophozoite Schizont Disease Cytoplasm Pigment Gametocyte
Merozole

Red blood cells

infected by P. vivas are Showing the large
Benign
often larger than numbers of merozotes Irregular, ameboid
Tertian;
uninfected red blood typical of this species introphozoite, has Golden brown, 12-24 Average
P. vivas Young Enlarge Schauffner’s merozoites rounded
cells. They are (16-24) Also note the spread out inconspicuous is 16
released every
approximately 1.5 larger size compared to appearance
48 hrs
times the size of a normal red blood cell
normal cell.
6-12 average is
Quartan; Rounded, compact
Containing mezozoites 8, “rosette”
merozoites trophozoite with dense Dark brown,
P. malariae Mature Normal Ziemann’s (6 to 12) giving a coarse schizonts are Rounded
released every cytoplasm/band from conspicuous
granular appearance sometimes
36-48 hrs. troph are rarely seen
seen
6-12 average is
Benign tertian; Rounded, compact
8, “rosette”
merozoites trophozoite with dense Dark brown,
P. ovale Young Enlarge Schauffner’s schizonts are Rounded
released every cytoplasm/band from consipicuous
sometimes
48 hrs. troph are rarely seen
seen
Early trophozoites
have the
Malignant
characteristics signet The gametocytes of P. Young rings are small,
tertian; Black coarse and
Normal/multiple ring. Also, unique to P. falciparum have a delicate, often with 6-32 average is Crescent or
P. falciparum All stage Maurer’s merozoites conspicuous in
infection falciparum is the crescent or banana double chromatin 20-24 banana
released every gametocytes
presence of multiple shape. mass
72 hrs.
trophozoites in one
cell.
 Notes on Philippine Important Amoeba Spp. Part 4 (Trypanosomes and Leismania spp.)
Disease Vector Portal of entry Infective stage Infective Stage for Vector

American
Break skin or intact
T. cruzi trypanosomiasis or Infected Triatomine bug Metacyclic Trypomastigote Trypomastigote
mucous
Chaga’s disease

Gambian or west
Break skin or intact
T. brucei gambience African Sleeping Infected Tse-Tse flies Metacyclic Trypomastigote Trypomastigote
mucous
Sickness
Rhodesian or west
Break skin or intact
T. brucei rhodesience African Sleeping Metacyclic Trypomastigote Trypomastigote
mucous
Sickness
Skin/Macrophages and
Visceral
Leishmania donovani Sand/Phlebotomus Fly monocytes of spleen, liver Promastigote Amastigote
Leishmanisis
bone marrow
Skin/Macrophages and
Cutaneous
Leishmania tropica Sand/Phlebotomus Fly monocytes of spleen, liver Promastigote Amastigote
Leishmaniasis
bone marrow
Mucocutananeous Skin/Macrophages and
Leishmania braziliense Leishmaniasis or Sand/Phlebotomus Fly monocytes of spleen, liver Promastigote Amastigote
ESPUNDIA bone marrow