Professional Documents
Culture Documents
Agricultural
Research
Service The Commercial Storage
Agriculture Handbook
Number 66 of Fruits, Vegetables, and
Revised February 2016
Florist and Nursery Stocks
United States Department of Agriculture
Agricultural
Research
Service
The Commercial Storage
Agriculture Handbook
Number 66
of Fruits, Vegetables, and
Florist and Nursery Stocks
Edited by Kenneth C. Gross, Chien Yi Wang, and Mikal Saltveit
_______________________________________________________________
Gross and Wang are formerly with the Food Quality Laboratory, Beltsville
Agricultural Research Center, USDA, Agricultural Research Service,
Beltsville, MD. They are now retired. Saltveit is with the Department of
Plant Sciences, University of California, Davis, CA.
Abstract The information contained in AH-66 has been
assembled from material prepared by nearly a
Gross, Kenneth C., Chien Yi Wang, and Mikal hundred authors from around the world. All of the
Saltveit, eds. 2016. The Commercial Storage of information contained herein was peer reviewed
Fruits, Vegetables, and Florist and Nursery Stocks. and edited for scientific content. Every effort was
Agriculture Handbook 66, U.S. Department made to provide the most accurate and current
of Agriculture, Agricultural Research Service, information available.
Washington, DC.
The contributors’ professional affiliations
Agriculture Handbook 66 (AH-66) represents and addresses were up-to-date at the time of
a complete revision and major expansion of submission of their chapters, and the editors
the 1986 edition. It has been reorganized and made all reasonable efforts to update any changes
now includes 17 Chapters and 138 Commodity received during the review and publishing process.
Summaries written by nearly a hundred experts However, due to the large number of contributors
in plant science and postharvest technology. and countries represented, it is not inconceivable
This version, like the previous editions of AH- that some of the contributors may have changed
66 in 1954, 1968, 1977, and 1986, presents organizations in the interim and thus are no
summaries of current storage requirements longer at the addresses given in this handbook. In
of fresh fruits, vegetables, cut flowers, and cases where the editors received specific address
other horticultural crops. However, this highly changes or death notices, all such updates are
expanded version also includes information on reflected in this volume.
quality characteristics, maturity indices, grading,
packaging, precooling, retail display, chilling Mention of trade names or commercial products
sensitivity, ethylene production and sensitivity, in this report is solely for the purpose of pro-
respiration rates, physiological disorders, viding specific information and does not imply
postharvest pathology, quarantine issues, and recommendation or endorsement by the U.S.
suitability as fresh-cut product. A large number Department of Agriculture.
of fruits and vegetables were added, as well
as sections on food safety, nutritional quality, This publication reports experimental results
texture, and fresh-cut produce. The purpose of and other information involving pesticides. It
storing plant material is to lengthen the time it does not contain recommendations for their use
can be consumed or utilized. In doing so, it is nor does it imply that uses discussed here have
critical to provide an environment that minimizes been registered. All uses of pesticides must be
deterioration, maintains microbial safety, and registered by appropriate State and/or Federal
retains other quality attributes. AH-66 provides agencies before they can be recommended.
guidelines and other important information for
storing and handling horticultural commodities While supplies last, printed copies of this
to accomplish this. publication may be obtained at no cost from the
USDA-ARS Food Quality Laboratory, Building
Keywords: carbon dioxide, chilling injury, 002, Room 117, 10300 Baltimore Avenue,
cold storage, controlled atmosphere storage, Beltsville, MD 20705-2350.
cut flowers, ethylene, flavor, food safety,
fresh-cut, fresh produce, fruit softening, heat Copies of this publication may be purchased
load, 1-methylcyclopropene, microbial safety, in various formats (microfiche, photocopy,
minimally processed, modified-atmosphere CD, and print on demand) from the National
packaging, potted plants, nutritional quality, nuts, Technical Information Service, 5285 Port Royal
orchids, packaging film, perishable, postharvest Road, Springfield, VA 22161, (800) 553-6847,
biology, precooling, respiration, sensory http//:www.ntis.gov.
evaluation, shelf-life, texture.
ii
This publication in its entirety is freely accessible
on the Internet at http://www.ars.usda.gov/is/np/
indexpubs.
ARS Mission
iv
Abbreviations lb pound, avoirdupois
RH relative humidity
APHIS Animal and Plant Health Inspection sec second
Service, USDA T short ton
ARS Agricultural Research Service, USDA STS silver thiosulfate
BTU British thermal unit NaOCl sodium hypochlorite
bu bushel SSC soluble solids content
CaCl2 calcium chloride SO2 sulfur dioxide
CDC Centers for Disease Control TA total acidity
and Prevention SSC total soluble solids
CO2 carbon dioxide U.S. United States
cm centimeter USDA United States Department
cfu colony forming units of Agriculture
CA controlled atmosphere VHT vapor heat treatment
CFM cubic feet per minute
ed., eds. editor; editors
EPA Environmental Protection Agency
FDA Food and Drug Administration, HHS
ft feet
GC gas chromatograph
GRAS generally recognized as safe
h hour
HAT high temperature forced-air treatment
Hg mercury
HHS United States Department of Health
and Human Services
HWB hot water brushing
HWT hot water treatment
in inch
J Joules
kg kilogram
L liter
MS mass spectrometer
m meter
mL milliliter
MA modified atmosphere
MAP modified atmosphere packaging
µL microliter
min minute
mo month
N Newtons
N2 nitrogen
nL nanoliter
oz ounce, Avoirdupois
O2 oxygen
OTR oxygen transmission rate
ppb parts per billion
ppm parts per million
% percent
v
vi
Contents Carrot.................................................................264
Cassava..............................................................268
Introduction............................................................1 Cauliflower.........................................................271
Temperature Conversion Chart..............................2 Celeriac..............................................................275
Metric Conversion Chart........................................3 Celery.................................................................277
Commodity Cross-Reference.................................4 Cherimoya..........................................................280
Respiration and Ethylene Production Rates...........7 Cherry (Sweet)...................................................282
Chicory (Belgian Endive or Witloof Chicory)...286
Postharvest Biology and Technology Chinese Cabbage................................................289
Precooling and Storage Facilities.........................11 Coconut..............................................................294
Heat Load Calculation.........................................19 Cranberry...........................................................297
Controlled Atmosphere Storage...........................22 Cucumber...........................................................302
Temperature Preconditioning...............................26 Currant, Gooseberry, and Elderberry.................306
Modified Atmosphere Packaging.........................42 Date....................................................................311
Wholesale Distribution Center Storage...............54 Dragon Fruit.......................................................315
Grocery Store Display Storage............................59 Durian................................................................318
Chilling and Freezing Injury................................62 Eggplant.............................................................322
Respiratory Metabolism.......................................68 Endive and Escarole...........................................326
Ethylene Effects...................................................76 Fennel.................................................................328
1-Methylcyclopropane (MCP).............................83 Fig......................................................................330
Texture.................................................................89 Garlic..................................................................333
Postharvest Pathology........................................111 Ginger................................................................336
Flavor.................................................................128 Ginseng..............................................................338
Food Safety........................................................149 Grape (American)..............................................340
Nutritional Quality and Its Grape (Muscadine).............................................342
Importance in Human Health.........................166 Grape (Table).....................................................344
Grapefruit...........................................................349
Commodity Summaries Greens for Cooking............................................353
-----Fruits and Vegetables----- Guava.................................................................356
Annual Culinary Herbs......................................173 Honeydew Melon...............................................358
Apple..................................................................176 Horseradish........................................................362
Apricot...............................................................195 Jerusalem Artichoke...........................................365
Arazá..................................................................198 Jicama................................................................369
Artichoke............................................................202 Kiwifruit.............................................................372
Asian Pear..........................................................205 Kohlrabi.............................................................377
Asparagus...........................................................210 Leek....................................................................379
Atemoya.............................................................214 Lemon................................................................382
Avocado.............................................................216 Lettuce................................................................386
Banana and Plantain...........................................224 Lime...................................................................390
Bean...................................................................230 Litchi..................................................................392
Beet....................................................................234 Longan...............................................................395
Blackberry..........................................................237 Longkong...........................................................398
Blueberry............................................................240 Loquat................................................................401
Bok Choy...........................................................243 Luffa...................................................................403
Breadfruit...........................................................245 Mandarin (Tangerine)........................................405
Broccoli..............................................................248 Mango................................................................408
Brussels Sprouts.................................................252 Mangosteen........................................................412
Cabbage..............................................................255 Mushroom..........................................................415
Carambola..........................................................261 Nectarine............................................................418
vii
Netted Melon.....................................................423 Tomatillo............................................................579
Nopalitos............................................................427 Tomato...............................................................581
Okra....................................................................430 Truffle.................................................................588
Olive...................................................................433 Turnip.................................................................590
Onion.................................................................436 Waterchestnut.....................................................592
Orange................................................................441 Water Convolvulus.............................................595
Papaya................................................................447 Watercress..........................................................597
Parsley................................................................453 Watermelon........................................................599
Parsnip...............................................................457 Wax Apple..........................................................602
Passion Fruit......................................................460
Pea......................................................................463 -----Fresh-Cut Produce-----
Peach..................................................................466 Fresh-Cut Fruits.................................................604
Pear....................................................................471 Fresh-Cut Vegetables.........................................624
Pepper................................................................481
Perennial Culinary Herbs...................................485 -----Floral Crops and Nursery Stock-----
Persimmon.........................................................487 Bedding Plants and Seedlings............................642
Pineapple............................................................491 Christmas Trees..................................................650
Plum and Fresh Prune........................................497 Cut Flowers and Greens.....................................659
Pomegranate.......................................................501 Flower Bulbs......................................................709
Potato.................................................................506 Flowering Potted Plants.....................................722
Prickly Pear........................................................ 511 Foliage Plants.....................................................725
Pumpkin and Winter Squash..............................514 Herbaceous Perennials.......................................727
Quince................................................................518 Orchids...............................................................732
Radicchio...........................................................521 Seeds and Pollen................................................735
Radish................................................................524
Rambutan...........................................................527 -----------Nuts-----------
Raspberry...........................................................530 Almond..............................................................744
Rhubarb..............................................................533 Hazelnut.............................................................748
Rutabaga............................................................535 Macadamia Nut..................................................754
Salad Greens......................................................538 Peanut.................................................................757
Salsify................................................................541 Pecan..................................................................768
Sapodilla and Related Fruit................................543 Pistachio.............................................................772
Black Sapote................................................543 Walnut................................................................777
Lucuma........................................................543
Mamey Apple...............................................544
Sapodilla......................................................544
Sapote...........................................................546
Star Apple.....................................................546
White Sapote................................................547
Southern Pea......................................................550
Sprouts...............................................................553
Squash................................................................556
Strawberry..........................................................560
Sweet Corn.........................................................562
Sweetpotato........................................................566
Swiss Chard.......................................................571
Tamarillo............................................................573
Tamarind............................................................575
Taro....................................................................577
viii
Introduction would like to express their sincere appreciation
to all of the contributors and to the reviewers,
This latest edition of Agriculture Handbook who are listed in the Acknowledgments.
66 (AH-66) represents a complete revision of
the 1986 edition. It has been reorganized and The original edition of AH-66, published in
now includes 17 chapters and 138 commodity 1954, was written by R.C. Wright, D.H. Rose,
summaries written by nearly a hundred experts and T.M. Whiteman, all from USDA Agricultural
in plant biology and postharvest technology. This Research Service (ARS). Then, in 1968, the
version—like the previous editions of AH-66 in handbook was revised by J.M. Lutz and R.E.
1954, 1968, 1977, and 1986—presents summaries Hardenburg, also from USDA-ARS. A major
of current storage requirements of fresh fruits, revision by R.E. Hardenburg, A.E. Watada, and
vegetables, cut flowers, and other horticultural C.Y. Wang, at USDA-ARS Horticultural Crops
crops. However, this highly improved and Quality Laboratory (now Food Quality Lab-
expanded version also includes information on oratory) in Beltsville, MD, was published in
quality characteristics, maturity indices, grading, 1986. In 1990, 10,000 copies of the 1986 edition
packaging, precooling, retail display, chilling were reprinted, of which few remain today. The
sensitivity, ethylene production and sensitivity, volume has also been translated into several
respiration rates, physiological disorders, languages. It was clearly time for an extensive
postharvest pathology, quarantine issues, and revision, both to bring the content up to date and
suitability as fresh-cut product. In addition, a large to increase its availability.
number of fruits and vegetables were added, as
well as sections on food safety, nutritional quality, Most temperatures are given in both ºC and ºF.
texture, and fresh-cut produce. Nevertheless, a “Temperature Conversion Chart”
is included in this volume. Though temperatures
The purpose of storing plant material is to are sometimes expressed to the first decimal place
lengthen the time it can be stored and marketed due to conversion, this does not mean that this
prior to consumption or other use. In doing so, it is level of accuracy is recommended, necessary, or
critical to provide an environment that minimizes possible in a commercial situation. Generally,
deterioration and maintains microbial safety and storage temperatures can only be expected
quality. The primary intent of AH-66 is to provide to be maintained within ±1 ºC. Also, see the
guidelines for optimal handling and storage of “Metric Conversion Chart” for some common
produce in order to accomplish this. metric conversions. Respiration and ethylene
production rates for many fruits and vegetables
AH-66 is intended as a general reference, and are also summarized in the sections “Respiration”
the recommendations should not be considered and “Ethylene Effects.” A“Commodity Cross-
absolute, but rather as safe limits at which Reference” index has been included to aid in
products can ordinarily be handled and stored. A finding the commodity summary for produce
draft version of the data presented in this volume called by various names in different cultures
is available at http://www.ba.ars.usda.gov/hb66. and geographical locations.
Updates to the online data will be made as they
become available, and users are encouraged to
periodically check for any new information. “In this work, when it shall be found that much is
omitted,let it not be forgotten that much likewise is
Each contribution in this volume was peer performed.”
reviewed by at least one individual knowledgeable Dr. Samuel Johnson,
in that particular area or commodity, as well as English lexicographer and essayist, 1775
two editors. This review process helped to ensure
that the information in this edition of AH-66 is
as accurate and current as possible. The editors
1
Temperature Conversion Chart
°C °F °C °F
-2 28.4 23 73.4
-1 30.2 24 75.2
0 32.0 25 77.0
1 33.8 26 78.8
2 35.6 27 80.6
3 37.4 28 82.4
4 39.2 29 84.2
5 41.0 30 86.0
6 42.8 31 87.8
7 44.6 32 89.6
8 46.4 33 91.4
9 48.2 34 93.2
10 50.0 35 95.0
11 51.8 36 96.8
12 53.6 37 98.6
13 55.4 38 100.4
14 57.2 39 102.2
15 59.0 40 104.4
16 60.8 45 113.0
17 62.6 50 122.0
18 64.4 55 131.0
19 66.2 60 140.0
20 68.0 65 149.0
21 69.8 70 158.0
22 71.6 75 167.0
2
Metric Conversion Chart
Mass
1.0 avoirdupois pound (lb) = 0.454 kilogram (kg) = 454 grams (g)
1.0 kilogram (kg) = 2.2 pounds (lb) = 35.2 avoirdupois ounces (oz) = 32.15 troy ounces
1.0 avoirdupois ounce (oz) = 0.9115 troy ounce = 0.0284 kilogram (kg) = 28.4 grams (g)
1 short ton (T) = 2,000 pounds (lb) = 907.2 kilograms (kg) = 0.893 long ton = 0.907 metric tonne
Length
1 inch (in) = 2.54 centimeters (cm)
1 centimeter (cm) = 0.394 inch (in)
1 foot (ft) = 30.48 centimeters (cm)
1 yard (yd) = 91.44 centimeters (cm) or 0.9144 meter (m)
1 meter (m) = 3.28 feet (ft) = 1.0936 yards (yd)
1 mile (mi) = 1.61 kilometers (km)
1 kilometer (km) = 0.621 mile (mi)
Volume
1 quart (qt) = 0.946 liter (L)
1 liter (L) = 1.057 quarts (qt)
1 cup (c) = 0.24 liter (L)
1 pint (pt) = 0.47 liter (L)
1 quart (qt) = 0.95 liter (L)
1 U.S. bushel (bu) = 35.24 liters (L)
1 liter (L) = 0.2838 bushel (bu)
1 U.S. gallon (gal) = 3.785 liters (L)
1 liter (L) = 0.2642 gallon (gal)
1 cubic foot (ft3) = 28.32 liters (L)
1 cubic yard (yd3) = 0.76 cubic meter (m3)
1 liter (L) = 61.02 cubic inches (in3)
Area
1 acre = 0.4047 hectare
1 hectare = 2.47 acres
1 square meter (m2) = 1550 square inches (in2) = 1.196 square yards (yd2) = 10.76 square feet (ft2)
1 square inch (in2) = 6.45 square centimeters (cm2)
1 square foot (ft2) = 0.0929 square meter (m2)
Energy/Work
1 joule (J) = 0.00094 British thermal units (BTU) = 1 watt per second (W s-1)
1 British thermal unit (BTU) = 1,055 joules (J) = 0.252 kilocalorie (kcal)
3
Commodity Cross-Reference To find... See...
Crenshaw melon Honeydew melon
To find... See... Custard apple Avocado
Abogado Avocado Daikon Radish
Alligator pear Avocado Dandelion Salad greens
Alfalfa sprouts Sprouts Dasheen Taro
Anon Sapodilla Date Plum Persimmon
Apple cactus Dragon fruit Dill Annual culinary herbs
Apple pear (misleading) Asian pear Dilly Sapodilla
Araçá boi Arazá Duku Longkong
Avocat Avocado Dulian Durian
Basil Annual culinary herbs Duren Durian
Bean sprouts Sprouts Duyin Durian
Beets Beet Eddoe Taro
Belgian endive Chicory Elderberry Currant
Bell pepper Pepper Escarole Endive and Escarole
Bhendi Okra Field salad Salad greens
Bhindi Okra Filbert Hazelnut
Boy-toyo Bok choy Fire dragon fruit Dragon fruit
Cactus fruit Prickly pear Flat bean Bean
Cactus pad Nopalitos French bean Bean
Cactus pear Prickly pear French sorrel Salad greens
Caimito Sapodilla Garden sorrel Salad greens
Calabaza Pumpkin Gooseberry Currant
Calabrese Broccoli Globe artichoke Artichoke
Canary melon Honeydew melon Collard greens Greens for Cooking
Cantelope Netted melon Gombo Okra
Casaba melon Honeydew melon Green bean Bean
Cassave Cassava Green cabbage Cabbage
Cay mang cut Mangosteen Green onion Onion
Chervil Annual culinary herbs Grosse sapote Sapodilla
Chico mamey Sapodilla Groundnut Peanut
Chico zapote Sapodilla Gumbo Okra
Chiku Sapodilla Hamburg parsley Parsley
Chile pepper Pepper Husk tomato Tomatillo
Chinese apple Pomegranate Japanese pear Asian pear
Chinese chard Bok choy Java plum Wax apple
Chinese chive Perennial culinary herbs Kadu Durian
Chinese date plum Persimmon Kale Greens for cooking
Chinese long bean Bean Kang kong Water convolvulus
Chinese okra Luffa Kong xin cai Water convolvulus
Chinese pear Asian pear La pitahaya rouge Dragon fruit
Chive Perennial culinary herbs Lady’s finger Okra
Ciku Sapodilla Lamb’s lettuce Salad greens
Claytonia Salad greens Langsat Longkong
Cocoyam Taro Lanson Longkong
Collards Greens for cooking Lychee Litchi
Coriander Annual culinary herbs Long bean Bean
Corn salad Salad greens Lucuma Sapodilla
4
To find... See... To find... See...
Malanga Taro Roquette Salad greens
Malay apple Wax apple Rose apple Wax apple
Mamey Sapodilla Rose water apple Wax apple
Mandioca Cassava Rosemary Perennial culinary herbs
Manggistan Mangosteen Round sorrel Salad greens
Mangis Mangosteen Rucola Salad greens
Mangkhut Mangosteen Rugula Salad greens
Mangostan Mangosteen Runner bean Bean
Mangostanier Mangosteen Rupina caspi Arazá
Mangostao Mangosteen Sage Perennial culinary herbs
Manggustan Mangosteen Salad chervil Annual culinary herbs
Manioc Cassava Salad pear Asian pear
Marjoram Perennial culinary herbs Sand apple Asian pear
Marmalade fruit Sapodilla Sapota Sapodilla
Matai Waterchestnut Sapote Sapodilla
Melon, Honeydew melon Honeydew melon Saurieng Durian
Melon, Netted Netted melon Savory Annual culinary herbs
Mesetor Mangosteen Savoy cabbage Cabbage
Miner’s lettuce Salad greens Sementah Mangosteen
Mongkhut Mangosteen Semetah Mangosteen
Mung bean sprouts Sprouts Sha Li pear Asian pear
Muskmelon Netted melon Shalea pear Asian pear
Mustard cabbage Bok choy Shallot Onion
Mustard greens Greens for cooking Snap bean Bean
Nachi Asian pear Sororia Arazá
Nasberry Sapodilla Sorrel Salad greens
Néspero Sapodilla Spearmint Perennial culinary herbs
Noplaes Nopalitos Spinach Greens for cooking
Oregano Perennial culinary herbs Sponge gourd Luffa
Oriental pear Asian pear Spring onion Onion
Oxheart cabbage Cabbage Sprouting broccoli Broccoli
Oyster plant Salsify Star apple Sapodilla
Pak-choy Bok choy Star fruit Carambola
Pake boong Water convolvulvus Stinkvrucht Durian
Pak-tsoi Bok choy Stinky rose Garlic
Palta Avocado Strawberry pear Dragon fruit
Paprika Pepper String bean Bean
Peppermint Perennial culinary herbs Sugar pea Pea
Pichi Arazá Summer savory Annual culinary herbs
Pitahaya Dragon fruit Summer squash Squash
Pitaya roja Dragon fruit Sunchokes Jerusalem artichoke
Pod bean Bean Swedes Rutabaga
Quaio Okra Swedish turnips Rutabaga
Quingumbo Okra Sweetsop Sapodilla
Rape Greens for cooking Sweet cherry Cherry, sweet
Red beet Beet Sweet pepper Pepper
Rian Durian Table beet Beet
Rocket salad Salad greens Taisai Bok choy
5
To find... See...
Tamarindo Tamarind
Tangerine Mandarin and Tangerine
Tannier Taro
Tarragon Perennial culinary herbs
Thang loy Dragon fruit
Thureen Durian
Thurian Durian
Thyme Perennial culinary herbs
Tree tomato Tamarillo
Turnip-rooted cabbage Kohlrabi or Rutabaga
Turnip greens Greens for cooking
Vegetable oyster Salsify
Water cabbage Water convolvulvus
White celery mustard Bok choy
White sapote Sapodilla
Whitloof Chicory
Winter purslane Salad greens
Winter spinach Water convolvulvus
Yard-long bean Bean
Yellow pitaya Dragon fruit
Yellow wax bean Bean
Yuca Cassava
Zapote Sapodilla
Zucchini Squash
6
Respiration and Ethylene Production Rates
The values in table 1 are approximations or the average rates of a range; see individual sections on each
commodity for more specific information and references. Values in parentheses after ethylene rates are the
temperatures at which ethylene production was measured. For respiration data, to get mL kg-1 h-1, divide
the mg kg-1 h-1 rate by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and 1.8 at 20 °C (68 °F). To calculate heat
production, multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 or by 61 to get kcal tonne-1 day-1.
7
Table 1. Rates of respiration and ethylene production—Continued
_________________________________________________________________________________
Respiration
Commodity _________________________________________ C2H4 Production
0 °C 5 °C 10 °C 15 °C 20 °C 25 °C
_________________________________________________________________________________
——————————mg kg-1 h-1————————- µL kg-1 h-1
8
Table 1. Rates of respiration and ethylene production—Continued
_________________________________________________________________________________
Respiration
Commodity _________________________________________ C2H4 Production
0 °C 5 °C 10 °C 15 °C 20 °C 25 °C
_________________________________________________________________________________
——————————mg kg-1 h-1————————- µL kg-1 h-1
9
Table 1. Rates of respiration and ethylene production—Continued
_________________________________________________________________________________
Respiration
Commodity _________________________________________ C2H4 Production
0 °C 5 °C 10 °C 15 °C 20 °C 25 °C
_________________________________________________________________________________
——————————mg kg-1 h-1————————- µL kg-1 h-1
10
Precooling and Storage Facilities stem-, and flower-type vegetables; melons; and
some tree fruits (Pentzer et al. 1936, Toussaint
James F. Thompson 1955, Stewart and Lipton 1960, Bennett 1963,
Perry and Perkins 1968, Mitchell 1971).
Thompson is with the Department of Biological &
Agricultural Engineering, University of California, Vacuum- and water spray vacuum-cooling
Davis, CA. He is now retired. are usually reserved for crops, such as leafy
vegetables, that release water vapor rapidly,
allowing them to be quickly cooled (Barger 1963,
In-Field Temperature Management Harvey 1963).
Temperature management of perishable Package icing uses crushed ice to cool and
commodities begins with proper handling at maintain product temperature and is used for
harvest. Generally, produce should be harvested a very few commodities, mainly those whose
in the morning so that it will be at the coolest purchasers have a strong traditional demand for
possible temperature during the delay between this method. It is still common for broccoli.
harvest and initial cooling. Exceptions to this
recommendation are produce, such as some Room cooling is accomplished by placing warm
citrus fruit, that are damaged if they are handled produce in a refrigerated room. Cooling times are
when they are turgid in the morning (Eckert and at least 24 h and can be much longer if produce is
Eaks 1989), or situations in which the produce is not packaged correctly or if no provision is made
harvested in the late afternoon so that it can be to allow airflow past boxes. It is used for a few
transported to a local market during the cool night commodities, such as citrus and CA-stored apples,
hours. Produce should be shaded to protect it from which can have acceptable, though not optimal,
solar heat gain. Reduce the time between picking quality without use of rapid cooling.
and initial cooling; this is particularly critical
because fruits and vegetables transpire and respire Transport cooling in refrigerated ships and
at high rates at field temperatures (Maxie et al. containers is used for products, such as bananas,
1959, Harvey and Harris 1986, d’Sousa and Ingle in areas with no cooling infrastructure. Highway
1989, Robbins and Moore 1992). trailers have insufficient airflow to cool produce
and should never be depended on for initial
cooling.
Initial Cooling Methods
Table 1 is a summary comparison of the six initial
Produce is usually cooled to its long-term storage cooling methods.
temperature in special facilities designed to
rapidly remove produce heat.
12
__________________________________________________________________________________________________________________
Forced-air Hydro Vacuum Water spray Ice Room
__________________________________________________________________________________________________________________
Typical cooling time (h) 1 to 10 0.1 to 1.0 0.3 to 2.0 0.3 to 2.0 0.1 to 0.3* 20 to 100
Product moisture loss (%) 0.1 to 2.0 0 to 0.5 2.0 to 4.0 No data No data 0.1 to 2.0
Water contact with product No Yes No Yes Yes, unless bagged No
Potential for decay contamination Low High† None High† Low Low
Capital cost Low Low Medium Medium High Low‡
Energy efficiency Low High High Medium Low Low
Water-resistant packaging needed No Yes No Yes Yes No
Portable Sometimes Rarely done
Common Common Common No
Feasibility of in-line cooling Rarely done Yes No No Rarely done No
__________________________________________________________________________________________________________________
Source: Thompson et al. 1998
*Top icing can take much longer.
†
Recirculated water must be constantly sanitized to minimize accumulation of decay-causing pathogens.
‡
Low if product is also stored in cooler as is done with apples; otherwise long cooling times make it an expensive system.
Forced-Air Cooling kg-1 sec-1 (1 L kg-1 sec-1 equals approximately
1 CFM lb-1). At 1 L kg-1 sec-1, grapes with a
Refrigerated air is used as the cooling medium small minimum diameter will cool in about 2 h,
with this system. It is forced through produce while cantaloupes with a much larger diameter
packed in boxes or pallet bins. A number of require more than 5 h. Boxes should have about
airflow systems are used, but the tunnel cooler is 5% sidewall vent area to accommodate airflow
the most common (Thompson et al. 1998). Two without excessive pressure drop across the box
rows of packages, bins, or palletized product are (Wang and Tupin 1968, Mitchell et al. 1971).
placed on either side of an air-return channel. A Internal packaging materials should be selected to
tarp is placed over the product and the channel, restrict airflow as little as possible.
and a fan removes air from the channel, drawing
air through the product. The product is cooled Forced-air cooling causes some moisture loss.
in batches. Cooling times range from 1 h for cut Loss may not be detectable for produce items
flowers to more than 6 h for larger fruit, packed in with a low transpiration coefficient, like citrus
airflow-restricting materials such as bags or paper fruits, or it may equal several percent of initial
wraps. weight for produce with a high transpiration
coefficient (Sastry and Baird 1978). Moisture loss
The cold-wall system is adapted to cooling is linearly related to difference between initial and
smaller quantities of produce (Thompson et al. final product temperatures. High initial produce
1998). Individual pallets or cartloads of packages temperatures cause higher moisture loss than
are placed against a plenum wall. Usually the lower temperatures when cooling starts. Moisture
plenum has a slightly lower air pressure than loss can be reduced at the expense of longer
the room, and air is pulled through the product. cooling times by wrapping product in plastic or
Some coolers, particularly for cut flowers, use a packing it in bags.
pressurized plenum and air is pushed through the
product. Cold-wall systems do not use floor space Details of fan selection, air plenum design,
as efficiently as tunnel coolers and require more refrigeration sizing, product cooling times, and
management because each pallet is operational guidelines can be found in Thompson
cooled individually. et al. (1998). Forced-air coolers are the least
energy efficient type of cooler but are widely
The serpentine air system is designed for cooling used because they are adaptable to a wide range
produce in pallet bins (Thompson et al. 1998). of products and packaging systems (Thompson
Stacks of even numbers of bins are placed against et al. 2002). Small units can be installed in many
a negative pressure plenum wall. Bottom openings existing cold storage facilities.
for forklift tines are used for air supply and air
return channels. Air flows vertically up or down
through the product. The forklift openings are Hydrocooling
limited in dimension, which restricts airflow
and causes slow cooling. This system is used for Cooling is accomplished with this technique
partially cooling product that will be packaged by moving cold water around produce with a
later and finish-cooled after packing and for shower system or by immersing produce directly
cooling product in long-term storage. The system in cold water. Shower coolers distribute water
uses cold room volume very efficiently. using a perforated metal pan that is flooded with
cold water from the refrigeration evaporator
Cooling time in forced-air coolers is controlled (Thompson et al. 1998). Shower-type coolers can
by volumetric airflow rate and product diameter be built with a moving conveyor for continuous
(Flockens and Meffert 1972, Gan and Woods flow operation, or they can be operated in a batch
1989). Coolers often operate with 1 L kg-1 sec1 mode. Immersion coolers are suited for produce
of produce, with a typical range of 0.5 to 2.0 L that sinks in water (Thompson et al. 1998). They
13
usually cool more slowly than shower coolers In addition, meltwater can damage neighboring
because water flows at slower rates past the produce in a shipment of mixed commodities.
product. Cut flowers are sometimes cooled initially with
a forced-air system, and a small amount of ice in
Water is a better heat-transfer medium than air, a sealed package is secured in the container. This
and consequently hydrocoolers cool produce much greatly reduces the amount of ice needed and
faster than forced-air coolers. In well designed eliminates meltwater damage, while providing
shower coolers, small diameter produce, like some temperature control during subsequent
cherries, cools in less than 10 min. Large diameter transit and handling.
products like melons cool in 45 to 60 min (Stewart
and Lipton 1960, Stewart and Couey 1963,
Thompson et al. 1998). Immersion coolers usually Vacuum Cooling
have longer cooling times than shower coolers
because water speed past produce is slower. This method achieves cooling by causing water
to rapidly evaporate from a product. Water loss
Packages for hydrocooled produce must allow of about 1% causes 6 °C (11 °F) product cooling
vertical water flow and tolerate water contact. (Barger 1963). Product is placed in a steel vessel
Plastic or wood containers work well in and vacuum pumps reduce pressure in the vessel
hydrocoolers. Corrugated fiberboard must be wax- from 760 mm Hg to 4.6 mm Hg (Thompson et al.
dipped to withstand water contact. (1998). Water boils at a pressure of 20 to 30 mm
Hg depending on temperature. This causes rapid
Hydrocoolers cause no moisture loss in cooling. moisture evaporation and produce cooling. At the
In fact, they can rehydrate slightly wilted produce. end of the cooling cycle, pressure equals 4.6 mm
Hydrocooler water spreads plant decay organisms Hg and water boils at 0 °C (32 °F). If the product
and thus must be obtained from a clean source is held at this pressure long enough, it will cool to
and treated (usually with hypochlorous acid 0 °C (32 °F). For produce that releases moisture
from sodium hypochlorite or gaseous chlorine) rapidly, like leafy green vegetables, cooling can
to minimize the levels of decay organisms be accomplished in 20 to 30 min, even when
(Thompson et al. 1998). the product is wrapped in plastic film (Cheyney
et al. 1979). The produce loses 2 to 4% of its
Calculations of hydrocooler size, refrigeration weight during cooling, depending on its initial
capacity, water flow needs, and typical product temperature. Spraying the produce with water
cooling times can be found in Thompson et al. before cooling minimizes product moisture loss.
(1998). Hydrocoolers can be fairly energy efficient Some coolers are fitted with water spray systems
and are the least expensive cooling method to that are activated during the cooling cycle.
purchase (Thompson 1992).
Procedures for estimating vacuum pump capacity,
refrigeration capacity, and condensing coil design
Package Icing can be found in Wang and Gitlin (undated). Use
Thompson et al. (1998) and assume a -9 to -7 °C
Packing a product with crushed or flaked ice can (15 to 20 °F) refrigerant evaporating temperature
quickly cool it and provides a source of cooling to estimate compressor horsepower. Vacuum
during subsequent handling. It also maintains high coolers are very energy efficient (Thompson et
humidity around the product, reducing moisture al. 1987) and are cost competitive if well utilized
loss. Its disadvantages are that it has high capital (Thompson 1992).
and operating costs, requires a package that will
withstand constant water contact, and usually
adds a great amount of weight to the package.
14
Marine Transport Cooling Cold Storage
16
pellets. A few products, especially floral and d’Sousa, M.C., and M. Ingle. 1989. Effect of
ornamental crops, can be chemically treated to delayed cooling on the poststorage flesh firmness
make them insensitive to ethylene damage. of apples. J. Food Sci. 54:493-494.
Controlled Atmosphere Facilities Eckert, J.L., and I.L. Eaks. 1989. Postharvest
disorders and diseases of citrus fruits. In W.
Storage rooms can be built for controlled Ruther, ed, The Citrus Industry, Vol. 5, pp.
atmosphere (CA) storage for about 5% additional 179-260, Department of Agriculture and
cost if they are properly designed initially. The Natural Resources, Pub. no. 3326, University of
extra cost is for sealing joints between walls, California, Davis, CA.
ceilings, and floors and for installing gas-tight
doors. Tilt-up concrete, metal panels, urethane Flockens, I.H., and H.F.T. Meffert. 1972.
foam, and plywood have all been successfully Biophysical properties of horticultural products as
used as gas barriers. These storage rooms also related to loss of moisture during cooling down. J.
need equipment for monitoring and controlling Sci. Food Agric. 23:285-298.
gas levels (Waelti and Bartsch 1990). Gan, G., and J.L. Woods. 1989. A deep bed
simulation of vegetable cooling. In V.A. Dodd and
References P.M. Grace, eds, Land and Water Use, pp. 2301-
2308. Balkema, Rotterdam, The Netherlands.
ASHRAE [American Society of Heating,
Refrigerating, and Air-Conditioning Engineers]. Harvey, J.M., and C.M. Harris. 1986. In-storage
1999. ASHRAE Handbook Series (4 books). softening of kiwifruit: effects of delayed cooling.
ASHRAE, Atlanta, GA. Int. J. Refrig. 9:352-355.
Baird, C.D., J.J. Gaffney, and M.T. Talbot. Harvey, J.M. 1963. Improved techniques for
1988. Design criteria for efficient and cost- vacuum cooling vegetables. ASHRAE J. 5:41-44.
effective forced-air cooling systems for fruits and
vegetables. ASHRAE Trans. 94:1434-1454. Heap, R. 1998. Transport of foodstuffs by sea.
In R. Heap, M. Kierstan, and G. Ford, eds., Food
Barger, W.R. 1963. Vacuum cooling: a comparison Transportation, pp. 75-96. Blackie Academic and
of cooling different vegetables. Marketing Professional, London, UK.
Research Report 600, U.S. Department of
Agriculture, Washington, DC. Kroca, R.W., and M.L. Hellickson. 1993. Energy
savings in evaporator fan-cycled apple storages.
Bennett, A.H. 1963. Thermal characteristics of Appl. Eng. Agric. 9:553-560.
peaches as related to hydro-cooling. Technical
Bull. 1292, U.S. Department of Agriculture, Maxie, E.C., F.G. Mitchell, and A. Greathead.
Washington, DC. 1959. Studies on strawberry quality. Calif. Agric.,
Feb. 1:16.
Cheyney, C.C., R.F. Kasmire, and L.L. Morris.
1979. Vacuum cooling of wrapped lettuce. Calif. Mitchell, F.G., R.A. Parsons, and G. Mayer. 1971.
Agric. 33:18-19. Cooling trials with plastic tray pack nectarines in
various containers. Calif. Agric. 25(9):13-15.
Crisosto, C.H., J.L Smilanick, N.K. Dokoozlian,
and D.A. Luvisi. 1994. Maintaining Table Grape Parsons, R.A., F.G. Mitchell, and G. Mayer. 1970.
Postharvest Quality for Long Distance Markets, Forced-air cooling of palletized fresh fruit. Paper
International Symposium on Table Grape 70-875, ASAE, St. Joseph, MI.
Production, American Society for Enology and
Viticulture, Anaheim, CA.
17
Parsons, R.A. 1972. Forced-air cooling of fruit in Thompson, J.F. 1999. Cold-storage systems. In
bulk bins. SP-01-72:38-41, American Society of F.W. Bakker-Arkema, ed., CIGR Handbook of
Agricultural Engineers, St. Joseph, MI. Agricultural Engineering, vol. IV, pp. 339-361.
ASAE, St. Joseph, MI.
Perry, R., and R. Perkins. 1968. Hydro-cooling
sweet corn. Paper 68-800, American Society of Thompson, J.F., F.G. Mitchell, and R.F. Kasmire.
Agricultural Engineers, St. Joseph, MI. 2002. Cooling horticultural commodities. In
A.A.Kader, ed., Postharvest Technology of
Pentzer, W.T., R.L. Perry, G.C. Hanna, et al. 1936. Horticultural Crops, pp. 97-112. DANR Pub. no.
Precooling and Shipping California Asparagus. 3311, University of California, Davis, CA.
University of California Agricultural Experiment
Station Bulletin 600, Univ. of California, Davis, Thompson, J.F., F.G. Mitchell., T.R. Rumsey, et al.
CA. 1998. Commercial cooling of fruits, vegetables,
and flowers. DANR Pub. no. 21567, University of
Rij, R. E., J.F. Thompson, and D. S. Farnham. California, Davis, CA.
1979. Handling, precooling, and temperature
management of cut flower crops for truck Toussaint, W.D., T.T. Hatlow, and G. Abshier.
transportation. USDA-SEA Adv. Agric. Technol. 1955. Hydro-cooling peaches in the North
AAT-W-5. June. Carolina sandhills. Env. Infor. Ser. no. 320, North
Carolina Agricultural Experiment Station, North
Robbins, J., and P.P. Moore. 1992. Fruit quality Carolina State University, Raleigh, NC.
of stored fresh red raspberries after a delay in
cooling. HortTechnology 2(4):468-470. Wang, J.K., and K. Tunpun. 1968. Forced-air
cooling of tomatoes in cartons. Trans. Am. Soc.
Sargent, S.A., M.T. Talbot, and J.K. Brecht. 1988. Agric. Eng. 12:804-806.
Evaluating precooling methods for vegetable
packinghouse operations. Proceedings of the Waelti, H., and J.A. Bartsch. 1990. CA storage
Florida State Horticulture Society 101:175-182. facilities. In M. Calderon and R. Barkai-Golan,
eds., Food Preservation by Modified Atmospheres,
Sastry, S.K., and C.D. Baird. 1978. Transpiration pp. 373-389. CRC Press, Boca Raton, FL.
rates of certain fruits and vegetables. ASHRAE
Trans. 84(2):237-255.
18
Heat Load Calculation
Some factors need to be considered in determining refrigeration required for a cold-storage plant.
Examples are simplified to illustrate steps necessary to calculate heat load of a refrigerated storage
area during cooling and normal storage operation. More information on load calculations can be found
in Patchen (1971), Ryall and Lipton (1979), ASHRAE (1981), and Bartsch and Blanpied (1984). The
information presented here is adapted from pages 14 to 16 of the previous USDA Agriculture Handbook
Number 66 (Hardenberg et al. 1986). Examples are shown in metric units for pears in storage at -1.1
ºC (30 ºF). To convert respiration rate of fruits and vegetables expressed in mg CO2 kg-1 h-1 to heat
production in kJ, multiply mg CO2 kg-1 h-1 by 61 to get kcal tonne-1 day-1 (1 kcal = 4,186 kJ).
Conditions Example
________________________________ _________________________________________
Storage size 15×15×4.5 m
Outside surface area (including floor) 720 m2
Inside dimensions 14.7×14.7×4.2 m
Volume 908 m3
Insulation 7.6 cm of polyurethane with a conductivity value
(k) = 1.3 kJ per m2 per cm thickness per ºC;
coefficient of transmission (U) = 1.1 kJ h-1 m-2 ºC-1
Ambient conditions at harvest 30 ºC and 50% RH
Fruit temperature at harvest, 21 ºC; in storage, -1.1 ºC
Storage capacity 600 bins at 500 kg fruit per bin = 300,000 kg of fruit
Bin weight 63.5 kg; total weight of bins = 38,100 kg
Loading weight and time 200 bins (100,000 kg fruit per day); 3 days to fill
Cooling rate 1st day, 21 to 4.5 ºC; 2nd day, 4.5 to -1.1 ºC
Air changes from door openings:
during cooling 6 per day
during storage 1.8 per day
Specific heat pears, 0.86; wood bins, 0.5
Heat load to lower air:
from 30 to -1.1 ºC (50% RH) 74.5 kJ m-3
from 7.2 to -1.1 ºC (70% RH) 15.3 kJ m-3
Miscellaneous heat loads lights, 2,400 W per h (3.6 kJ W-1)
fans at 3,112 kJ per HP
electric forklifts, 36,920 kJ each for 8 h
workers, 1,000 kJ per h per person
19
A. Load during cooling and filling storage: temperature difference (TD) from 30 ºC to -1.1 ºC = 31.1
ºC, assuming 31.1 ºC TD on all surfaces:
kJ per 24 h
1. Building-transmission load: area (720 m2) × U (1.1 kJ) × TD (31.1 ºC) × h (24) = 591,149
2. Air-change load from doors: vol (908 m3) × heat load (74.5 kJ) × air changes (6) = 405,876
Subtotal 11,399,302
Add 10% to be cautious 1,139,930
Total required refrigeration 12,539,232
20
Assuming refrigeration equipment operates 18 h per day: 12,539,232 ÷ 18 h = 696,624 kJ h-1. Since a
tonne of refrigeration absorbs 12,660 kJ per 24 h: 696,624 ÷ 12,660 = 55 tonnes of peak refrigeration
capacity is required.
B. Load during normal storage operation (average outside ambient conditions, 7.2 ºC at 70% RH;
storage temperature, -1.1 ºC; TD = 7.2 º to -1.1 ºC = 8.3 ºC).
kJ per 24 h
2
1. Building-transmission load: area (720 m ) × U (1.1 kJ) × TD (8.3 ºC) × h (24) = 157,766
2. Air-change load from doors: vol (908 m3) × heat load (15.3 kJ) × air changes (1.8) = 25,006
Subtotal 688,996
Add 10% to be cautious 68,899
Total required refrigeration 757,895
Assuming refrigeration equipment operates 18 hours per day: 757,895 ÷ 18 h = 42,105 kJ h-1 and
42,105 ÷ 12,660 = 3.3 tonnes of refrigeration capacity is needed during normal storage.
21
Controlled Atmosphere Storage fruits and vegetables are able to recover from
the detrimental effects of low O2 and high CO2
Adel A. Kader stresses (fermentative metabolism) and resume
normal respiratory metabolism upon transfer to
Kader was with the Department of Plant Sciences, air. Plant tissues have the capacity for recovery
University of California, Davis, CA. He is from the stresses caused by brief exposure
deceased. to fungistatic atmospheres (>10% CO2) or
insecticidal atmospheres (<1% O2 and/or 40 to
80% CO2). Postclimacteric fruits are less tolerant
Introduction and have lower capacity for recovery following
exposure to reduced O2 or elevated CO2 levels
than preclimacteric fruits. The speed and extent of
Controlled atmosphere (CA) storage involves recovery depend on duration and levels of stresses
maintaining an atmospheric composition that is and underlying, metabolically driven cellular
different from air composition (about 78% N2, repair.
21% O2, and 0.03% CO2); generally, O2 below
8% and CO2 above 1% are used. Atmosphere Elevated-CO2 atmospheres inhibit activity of
modification should be considered as a supplement ACC synthase (key regulatory site of ethylene
to maintenance of optimum ranges of temperature biosynthesis), while ACC oxidase activity is
and RH for each commodity in preserving quality stimulated at low CO2 and inhibited at high CO2
and safety of fresh fruits, ornamentals, vegetables, concentrations and/or low O2 levels. Ethylene
and their products throughout postharvest action is inhibited by elevated CO2 atmospheres.
handling. This chapter gives an overview of Optimum atmospheric compositions retard
responses to CA; specific CA considerations are chlorophyll loss (green color), biosynthesis
given in individual commodity summaries. of carotenoids (yellow and orange colors)
and anthocyanins (red and blue colors), and
biosynthesis and oxidation of phenolic compounds
Biological Basis of CA Effects (brown color). Controlled atmospheres slow
down the activity of cell wall degrading enzymes
Exposure of fresh horticultural crops to low O2 involved in softening and enzymes involved in
and/or elevated CO2 atmospheres within the lignification, leading to toughening of vegetables.
range tolerated by each commodity reduces Low O2 and/or high CO2 atmospheres influence
their respiration and ethylene production rates; flavor by reducing loss of acidity, starch to
however, outside this range respiration and sugar conversion, sugar interconversions, and
ethylene production rates can be stimulated, biosynthesis of flavor volatiles. When produce
indicating a stress response. This stress can is kept in an optimum atmosphere, ascorbic acid
contribute to incidence of physiological disorders and other vitamins are retained, resulting in better
and increased susceptibility to decay. Elevated nutritional quality.
CO2-induced stresses are additive to and
sometimes synergistic with stresses caused by low Severe stress CA conditions decrease
O2, physical or chemical injuries, and exposure cytoplasmic pH and ATP levels and reduce
to temperatures, RH, and/or C2H4 concentrations pyruvate dehydrogenase activity, while pyruvate
outside the optimum range for the commodity. decarboxylase, alcohol dehydrogenase, and lactate
dehydrogenase are induced or activated. This
The shift from aerobic to anaerobic respiration causes accumulation of acetaldehyde, ethanol,
depends on fruit maturity and ripeness stage ethyl acetate, and/or lactate, which may be
(gas diffusion characteristics), temperature, detrimental to the commodities if they are exposed
and duration of exposure to stress-inducing to stress CA conditions beyond their tolerance.
concentrations of O2 and/or CO2. Up to a point, Specific responses to CA depend on cultivar,
22
maturity and ripeness stage, storage temperature • Low O2 (<1%) and/or elevated CO2 (40 to
and duration, and in some cases ethylene 60%) can be a useful tool for insect control
concentrations. in some fresh and dried fruits, flowers, and
vegetables and in dried nuts and grains.
N2 is an inert component of CA. Replacing N2
with argon or helium may increase diffusivity of
O2, CO2, and C2H4, but they have no direct effect Detrimental Effects of CA (Above or
on plant tissues and are more expensive than N2 as Below Optimum Composition for the
a CA component. Commodity)—A Summary
23
atmospheric composition around and within the sweet onions, kiwifruits, avocados, persimmons,
commodity. Modified atmosphere packaging pomegranates, and nuts and dried fruits and
(MAP) is widely used in marketing fresh-cut vegetables (table 1). Atmospheric modification
produce. during long-distance transport is used with
apples, asparagus, avocados, bananas, broccoli,
Applications of CA to cut flowers are very limited cane berries, cherries, figs, kiwifruits, mangos,
because decay caused by Botrytis cinerea is often melons, nectarines, peaches, pears, plums,
a limiting factor to postharvest life, and fungistatic and strawberries. Continued technological
CO2 levels damage flower petals and/or associated developments in the future to provide CA
stem and leaves. Also, it is less expensive to treat during transport and storage at a reasonable cost
flowers with anti-ethylene chemicals than to use (positive benefit/cost ratio) are essential to greater
CA to minimize ethylene action. applications on fresh horticultural commodities
and their products.
Commercial use of CA storage is greatest on
apples and pears worldwide, less on cabbages,
>12 Almond, Brazil nut, cashew, filbert, macadamia, pecan, pistachio, walnut,
dried fruits and vegetables
1 to 3 Avocado, banana, cherry, grape (no SO2), mango, olive, onion (sweet
cultivars), some cultivars of nectarine, peach and plum, tomato (mature-green)
24
Additional Reading and Reference Material Thompson, A.K. 1998. Controlled Atmosphere
Storage of Fruits and Vegetables. CAB
Calderon, M., and R. Barkai-Golan, eds. 1990. International, Wallingford, U.K.
Food Preservation by Modified Atmospheres.
CRC Press, Boca Raton, FL. Thompson, J.F., and E.J. Mitcham, eds. 1997.
Technology and Disinfestation Studies, CA’97
El-Goorani, M.A., and N.F. Sommer. 1981. Proceedings, vol. 1. Postharvest Hort. Ser. no. 15,
Effects of modified atmospheres on postharvest University of California, Davis, CA.
pathogens of fruits and vegetables. Hort. Rev.
3:412-461. Vigneault, C., V.G.S. Raghavan, and R. Prange.
1994. Techniques for controlled atmosphere
Gorny, J., ed. 1997. Fresh-Cut Fruits and storage of fruit and vegetables. Tech. Bull 1993-
Vegetables and MAP, CA’97 Proceedings, vol. 18E, Agriculture Canada, Kentville, N.S.
5. Postharvest Hort. Ser. no. 19, University of
California, Davis, CA. Wang, C.Y. 1990. Physiological and biochemical
effects of controlled atmosphere on fruits and
Kader, A.A. 1986. Biochemical and physiological vegetables. In M. Calderon and R. Barkai-Golan,
basis for effects of controlled and modified eds, Food Preservation by Modified Atmospheres,
atmospheres on fruits and vegetables. Food pp. 197-223. CRC Press, Boca Raton, FL.
Technol. 405:99-100, 102-104.
Weichmann, J. 1986. The effect of controlled
Kader, A.A., ed. 1997. Fruits Other Than Apples atmosphere storage on the sensory and nutritional
and Pears, CA’97 Proceedings, vol. 3. Postharvest quality of fruits and vegetables. Hort. Rev. 8:101-
Hort. Ser. no. 17, University of California, Davis 127.
CA.
Yahia, E.M. 1998. Modified and controlled
Kader, A.A., D. Zagory, and E.L. Kerbel. 1989. atmosphere for tropical fruits. Hort. Rev. 22:123-
Modified atmosphere packaging of fruits and 183.
vegetables. CRC Crit. Rev. Food Sci. Nutr. 28:1-
30. Zagory, D., and A.A. Kader. 1989. Quality
maintenance in fresh fruits and vegetables by
Mitcham, E.J., ed. 1997. Apples and Pears, CA’97 controlled atmospheres. In J.J. Jen, ed., Quality
Proceedings, vol. 2. Postharvest Hort. Ser. no. 16, Factors of Fruits and Vegetables: Chemistry and
University of California, Davis, CA. Technology, pp. 174-188. American Chemical
Society, Washington, DC
Raghavan, G.S.V., P. Alvo, Y. Gairepy, and C.
Vigneault. 1996. Refrigerated and controlled
modified atmosphere storage. In L.P. Somogyi
et al., eds, Processing Fruits: Science and
Technology, vol. 1, Biology, Principles and
Applications, pp. 135-167. Technomic Pub. Co.,
Lancaster, PA.
25
Temperature Preconditioning of temperature manipulation is often referred to
as a “curing” period and is used with crops such
Susan Lurie and Joshua D. Klein as potatoes, onions, and carrots. Its purpose is
generally to increase resistance of the commodity
Lurie is with the Department of Postharvest to pathogen invasion, though it may also increase
Science, Volcani Center, Bet-Dagan, Israel; and resistance to low-temperature injury in citrus.
Klein is with the Department of Field Crops,
Volcani Center, Bet-Dagan, Israel. In this chapter we discuss temperature
preconditioning treatments according to their
purpose; that is, pathogen, insect, or chilling
Introduction injury control. Most of the methods listed here,
however, are still experimental and have yet to be
Temperature preconditioning of fruits and accepted for routine commercial practice.
vegetables has been practiced for more than 70
years, since Baker (1939, 1952) described heat
treatments for disinfestation of fruit flies in citrus. Commercial Treatments
There is renewed interest in high temperature as
a postharvest treatment for control of both insect The greatest number of temperature manipulations
pests and fungal pathogens in fresh produce. In used commercially are based on high-temperature
part, this is because of the deregistration of a treatments (vapor heat or hot forced-air) for insect
number of compounds that, until recently, have disinfestation. Temperature regimes are developed
been used for effective control of postharvest specifically for each commodity and insect pest.
disorders. In addition, there is increased consumer The accepted procedures for produce entering
demand for produce that has had minimal, or the United States are described in the Plant
ideally no, chemical treatment. Protection and Quarantine Treatment Manual
from USDA, Animal and Plant Health Inspection
Heat has fungicidal as well as insecticidal action, Service (APHIS). The manual is routinely updated
but heat regimes that are optimal for insect control (APHIS 1998). The latest edition of the manual
may not be optimal for disease control; in some should be consulted for approved treatments for
cases they may even be detrimental. A thermal particular commodities or pests.
treatment that is developed for fungus or insect
control should not damage the commodity being An example of commercial temperature
treated. In fact, in many cases high temperature conditioning for pest control is Mexican-grown
manipulation before storage may have beneficial mangos, which may be infested with a variety
effects on the commodity treated. These benefits of fruit fly larvae or eggs. Officially authorized
include slowing the ripening of climacteric fruit treatments are high-temperature, forced-air
and vegetables, enhancing sweetness of produce treatment (HAT) or a hot water dip treatment
by increasing the amount of sugars or decreasing (HWT) before storage and shipment. In HAT, fruit
acidity, and prevention of storage disorders such are heated until their centers reaches 48 °C (118
as superficial scald on apples and chilling injury °F). HWT conditions depend on fruit size and can
on subtropical fruits and vegetables (Lurie 1998). vary from 45 to 90 min in water, where the fruit
interior reaches 46 °C (115 °F).
Temperature conditioning before storage may also
mean an incubation period spent at either ambient Vapor-heat (VHT) differs from high-temperature,
temperature of 16 to 25 °C (61 to 77 °F) or at a forced-air in that moisture accumulates on the
temperature below ambient but above that which surface of the fruit. The water droplets transfer
might produce chilling injury: 5 to 12 °C (41 to heat more efficiently than air, allowing the fruit
54 °F), depending on the commodity. This type to heat quickly; but there may also be increased
26
physical injury to the fruit. Papayas grown in develop fewer rots after storage. Onions can be
Hawaii are vapor-heat-treated before being stored longer if held at 28 °C (82 °F) for 3 days
exported to Japan. before storage.
Citrus can be disinfested by HAT at 44 °C (111 The two commercial applications of high-
°F) for 100 min, with an additional 90 min temperature antifungal treatments are HWT for
spent raising the temperature to 44°C. The usual papayas (Akamine and Arisumi 1953), which has
disinfestation method, however, is to hold the fruit been used for almost 50 years, and a hot-water
at low temperature of 0 to 2 °C (32 to 36 °F) for brush treatment (HWB) (Fallik 1996a, 1999,
10 to 16 days before raising the temperature to the Prusky et al. 1997). The brush system is in use
normal storage temperature of 6 to 11°C (43 to 52 on packing lines for export of corn, mangos,
°F), depending on cultivar. Since citrus is sensitive peppers, and some citrus from Israel. The machine
to chilling, fruit are generally held at 20 °C (68 °F) sprays hot water at 50 to 65 °C (122 to 149 °F) on
or 16 °C (61 °F) for 3 to 5 days before placing at produce as it moves along on brush rollers. The
low temperature. This curing treatment decreases major benefit appears to be removal of spores and
fruit susceptibility to chilling injury resulting from dirt, though hot water combined with brushing
the subsequent disinfestation treatment. also causes surface cracks to be filled in by the
natural wax of the commodity, as well as eliciting
resistance to pathogens in some cases.
Insect Disinfestation
The state of temperature conditioning treatments
The development and implementation of heat against fungal pathogens was reviewed by Barkai-
treatments for insect disinfestation have been Golan and Phillips (1991) and Coates and Johnson
reviewed thoroughly (Couey 1989, Paull 1993). (1993). The majority of the regimes listed in table
Table 1 includes treatment regimes that have 2 were developed in the past 10 years. Dips in
been reported in the past 20 years. More than half hot fungicide solution have been used since the
the treatments are designed to kill fruit fly eggs 1950s for pathogen control. As various fungicides
or larvae, because their presence requires strict lose their registration or as pathogens develop
quarantine in most fruit-importing countries. The resistance, there is increased interest in heat-
most recently developed methods include heat treating produce in combination with compounds
treatments in combination with low-O2 or high- that are generally recognized as safe (GRAS),
CO2 atmospheres. such as calcium chloride or sodium carbonate
(table 2).
Antifungal Treatments
Physiological Benefits of Conditioning
Curing is used commercially to increase resistance Treatments
to pathogen invasion. Potatoes are cured at 12 °C
(54 °F) for 10 to 12 days before storage at 4 to 9 Most thermal treatments have been developed
°C (39 to 48 °F), depending on cultivar and on as lethal regimes for insects or fungi. Some of
whether they are designated for industry or home these regimes, however, also have prophylactic
consumption. Sweet potatoes are also cured at 30 effects against physiological disorders such as
°C (86 °F) for 5 days before storage at 12 °C (54 chilling injury (CI). Prevention of CI allows
°F). In both cases the curing period allows for the commodity to be stored longer at lower
wound healing and deposition of cell wall material temperatures, which in turn permits export in ships
to create a physical barrier to pathogens. Kiwifruit rather than more costly air freight. In addition,
also benefit from a curing period. If held at 10 °C a preshipping heat treatment can allow for low-
(50 °F) before storage at low temperatures, they temperature disinfestations of commodities, such
27
as citrus, by improving the resistance of fruit to CI
generally incurred during this treatment.
28
Table 1. Thermal treatment of horticultural commodities for insect disinfestation
_____________________________________________________________________________________________________________________
Fruit flies
Caribbean fruit fly Anastrepha suspensa grapefruit HAT Sharp & Gould 1994
mango HAT 52 °C/125 min Miller et al. 1991
orange HAT Sharp & McGuire 1996
Mediterranean fruit fly Ceratitis capitata avocado HAT 40 °C/24 h Jang 1996
mango VHT 47 °C/15 min Heather et al. 1997
papaya HAT 47 °C at pulp for 3.5 h Armstrong et al. 1995
Melon fruit fly Dacus cucurbitae avocado HAT 40 °C/24 h Jang 1996
Bactrocera cucurbitae
cucumber HAT then HWT 32 °C/24 h then Chan & Linse 1989
45-46 °C/50-60 min
papaya HAT 4C °C at pulp for 3.5 h Armstrong et al. 1995
zucchini VHT Jacobi et al. 1996
Mexican fruit fly Anastrepha ludens grapefruit HAT & CA 44 °C/2 h in 1% O2 Shellie et al. 1997
Bactrocera cucumis
zucchini VHT 45 °C/30 min Jacobi et al. 1996
Oriental fruit fly Dacus dorsalis cucumber HAT then HWT 32 °C/24 h then Chan & Linse 1989
Bactrocera dorsalis 45-46 °C/50-60 min
papaya HAT 47 °C at pulp for 3.5 h Armstrong et al. 1995
Papaya fruit fly Bactrocera payapae mango VHT 47 °C/15 min Heather et al. 1997
Queensland fruit fly Bactrocera tyroni avocado HWT & benomyl 46 °C/3 min then Jessup 1994
1 °C/7 days
litchi VHT 45 °C/30 min Jacobi et al. 1993
mango VHT 47 °C/10 min Heather et al. 1997
Jacobi et al. 1995
HWT then VHT 53 °C/15 min then Jacobi & Giles 1997
29
47°C/15 min
Table 1. Thermal treatment of horticultural commodities for insect disinfestation—Continued
30
_____________________________________________________________________________________________________________________
Other insects
Coddling moth Cydia pomonella apple HAT or VHT 44 °C/120 min then Neven et al. 1996
0 °C/4 weeks
cherry HAT & CA 47 °C/44 min in 1% O2; Neven & Mitcham 1996
15% CO2 Neven & Drake 2000
pear HAT or VHT 44 °C/120 min then Neven et al. 1996
0°C/4 weeks
HAT & CA 30 °C/ 30 h in 0.3% O2 Chervin et al. 1997
Fuller’s rose beetle Asynonychus godmani lemon HWT 52 °C/8 min Soderstrom et al. 1993
Leafroller Cnephasia jactatana apple HAT & CA 40 °C/10 h in 0.4% O2 Whiting et al. 1999
Ctenopseustis 45 °C/5 h in 0.4% O2
obliquana kiwifruit HAT & CA 40 °C/5-7 h in 0.4% O2 Whiting et al. 1997
40 °C/6 h in 2% O2; Hoy & Whiting 1998
5% CO2
Light brown apple moth Epiphyas postvittana apple HAT & CA 40 °C/17-20 h in Lay-Yee et al. 1997
1.2% O2; 1% CO2 Dentener et al. 2000
HWT & ethanol 45 °C/13 min in Hoy & Whiting 1998
50% ethanol
HAT & CA
kiwifruit
pear HAT & CA 30 °C/30 h in 0.3% O2 Chervin et al. 1997
Longtailed mealybug Pseudococcus longispinus persimmon HWT 48 °C/26 min or Lester et al. 1995
50 °C/22 min
HAT Dentener et al. 1996,
1997
Table 1. Thermal treatment of horticultural commodities for insect disinfestation—Continued
_____________________________________________________________________________________________________________________
New Zealand flower thrips Thrips obscuratus apricot HWT 48 °C/3 min then McLaren et al. 1997
nectarine 50 °C/2 min
peach
Obscure mealybug Pseudococcus affinis apple HAT & CA 40 °C/10 h in 0.4% O2 Whiting & Hoy 1997
45 °C/5 h in 0.4% O2
Oriental fruit moth Grapholita molesta pear HAT & CA 30 °C/30 h in 0.3% O2 Chervin et al. 1997
Two spotted spider mite Tetranychus urticae apples HWT & ethanol 45 °C/13 min in 50% Dentener et al.
ethanol 1998
kiwifruit HAT & CA 44 °C/211 min Lay-Yee & Whiting
1996
persimmon HWT 47 °C/67 min Lester et al. 1997
White peach scale Pseudaulacaspis pentagona papaya VHT 47 °C/4 h Follet & Gabbard 1999
_____________________________________________________________________________________________________________________
31
32
Table 2. Thermal treatment of horticultural commodities for eradication of and protection from fungal pathogens
_____________________________________________________________________________________________________________________
Alternaria alternata Black spot carrot HWB 100 °C/3 sec Afek et al. 1999
mango HWB 60-70 °C/15-20 sec Prusky et al. 1999
Black mold pepper HWT 50 °C/3 min Fallik et al. 1996b
Botrytis cinerea Grey mold apple HAT & CaCl2 38 °C/4 days and CaCl2 dip Klein et al. 1997
pepper HWT 50 °C/3 min Fallik et al. 1996b
strawberry HWT 45 °C/15 min Garcia et al. 1996
tomato HWT 50 °C/2 min Barkai-Golan et al. 1993
HAT 38 °C/2 days Fallik et al. 1993
Botryodiplodia Stem and surface rots papaya HAT 49 °C/20 min Nishijima et al. 1992
theobromae 32 °C/30 min then
49 °C/20 min
Chalara paradoxa Crown rot banana HWT 45 °C/20 min or Reyes et al. 1998
50 °C/10 min
Colletotrichum Anthracnose mango VHT 46-48 °C/24 sec to 8 min Coates et al. 1993
gloeosporioides HWT McGuire 1991
HAT 51.5 °C/125 min Miller et al. 1991
Diplodia natalensis Stem end rot mango HAT 51.5 °C/125 min Miller et al. 1991
HWT McGuire 1991
Mycospharella spp. Stem and surface rots papaya HAT 49 °C/20 min Nishijima et al. 1992
42 °C/30 min then
49°C/20 min
Penicillium expansum Blue mold apple HAT & CaCl2 38 °C/4 days in 4% CaCl2 Sams et al. 1993
HAT 38°C/4 days Fallik et al. 1996c
Penicillium italicum Blue mold cactus pear HAT or HWT 38 °C/24 h or 55 °C/5 min Schirra et al. 1996
Penicillium spp. lemon HWT & imazalil 50 °C/3 min + imazalil Schirra et al. 1997
33
Table 3. Physiological benefits of thermal treatments for horticultural crops
____________________________________________________________________________________
Chilling injury
____________________________________________________________________________________
Avocado skin browning HAT then 38 °C/3-10 h then Woolf et al. 1995
internal browning, HWT 40 °C/30 min Florissen et al. 1996
pitting 38 °C/60 min Woolf et al. 1997
HWT
Cactus pear rind pitting, HAT or HWT 38 °C/24 h or Schirra et al. 1996
brown staining 55 °C/5 min
34
Table 3. Physiological benefits of thermal treatments for horticultural crops—Continued
____________________________________________________________________________________
Apple increased firmness HAT 38 °C/4 days; Klein & Lurie 1992
42 °C/2 days
Asparagus inhibited curvature HWT 47 ºC/2-5 min Paull & Chen 1999
Green onions inhibited elongation HWT 55 °C/2 min Hong et al. 2000
35
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41
Modified Atmosphere Packaging aerobic respiration (Kays 1997). Similarly, injury
will occur if CO2 exceeds tolerable levels. Ranges
Nazir Mir and Randolph M. Beaudry of nondamaging O2 and CO2 levels have been
published for a number of fruits and vegetables
Mir and Beaudry are with Michigan State (Kader 1997a, Kupferman 1997, Richardson and
University, East Lansing, MI. Kupferman 1997, Saltveit 1997, Beaudry 1999,
2000), minimally processed products (Gorny
1997), and flowers and ornamentals (Reid 1997).
Introduction Horticultural crops differ in their tolerance for
O2 (table 1) and CO2 (table 2). ). The range of O2
Modified-atmosphere packaging (MAP) of fresh and CO2 levels for fruits (figure 1) and vegetables
fruits and vegetables refers to the technique of (figure 2) can overlap or be distinct.
sealing actively respiring produce in polymeric
film packages to modify the O2 and CO2 levels
within the package atmosphere. It is often
desirable to generate an atmosphere low in O2 and/
or high in CO2 to influence the metabolism of the
product being packaged or the activity of decay-
causing organisms to increase storability and/or
shelf-life. For some products, modifying both O2
and CO2 may be desirable; indeed, altering the O2
level automatically alters CO2 level. In addition to
atmosphere modification, MAP vastly improves
moisture retention, which can have a greater
influence on preserving quality than O2 and
CO2 levels. Furthermore, packaging isolates the
product from the external environment and helps
to ensure conditions that, if not sterile, at least
reduce exposure to pathogens and contaminants.
O2 Commodities
%
<0.5 Chopped greenleaf*, redleaf*, romaine* and iceberg lettuce*; spinach; sliced
pear; broccoli*; mushroom
1.0 Broccoli florets, chopped butterhead lettuce, sliced apple, brussels sprouts,
cantaloupe, cucumber, crisphead lettuce, onion bulbs, apricot, avocado,
banana*, cherimoya, atemoya, sweet cherry, cranberry, grape, kiwifruit*,
litchi, nectarine, peach, plum, rambutan, sweetsop
1.5 Most apples*, most pears*
2.0 Shredded and cut carrots, artichoke, cabbage*, cauliflower, celery, bell and
chili peppers, sweet corn, tomato, blackberry, durian, fig, mango, olive,
papaya, pineapple, pomegranate, raspberry, strawberry
2.5 Shredded cabbage, blueberry
3.0 Cubed or sliced cantaloup, low-permeability apples* and pears*, grapefruit,
persimmon
4.0 Sliced mushrooms
5.0 Green snap beans, lemon, lime, orange
10.0 Asparagus
14.0 Orange sections
Source: Gorny 1997, Kader 1997a, Kupferman 1997, Richardson and Kupferman 1997, Saltveit
1997, and Beaudry 2000
* Considered to have very good to excellent potential to respond to low O2.
43
Table 2. CO2 partial pressures above which injury will occur for selected
horticultural crops
______________________________________________________________________
CO2 Commodities
______________________________________________________________________________
kPa
2 Lettuce (crisphead), pear
3 Artichoke, tomato
5 Apple (most cultivars), apricot, cauliflower, cucumber, grape, nashi, olive,
orange, peach (clingstone), potato, pepper (bell)
7 Banana, bean (green snap), kiwifruit
8 Papaya
10 Asparagus, brussels sprouts, cabbage, celery, grapefruit, lemon, lime, mango,
nectarine, peach (freestone), persimmon, pineapple, sweet corn
15 Avocado, broccoli, lychee, plum, pomegranate, sweetsop
20 Cantaloupe (muskmelon), durian, mushroom, rambutan
25 Blackberry, blueberry, fig, raspberry, strawberry
30 Cherimoya
______________________________________________________________________________
Source: modified from Herner 1987, Kader 1997a, and Saltveit 1997
Figure 1. Recommended O2 and CO2 combinations for the Figure 2. Recommended O2 and CO2 combinations for
storage of fruit. The chart depicts atmospheres theoretically the storage of vegetables. The chart depicts atmospheres
attainable by MAP by film permeation alone (low-density theoretically attainable by MAP using low-density
polyethylene, LDPE, lower boundary) and via perforations polyethylene (LDPE, lower boundary) and perforated
alone (upper, dashed line) or their combination (shaded packages (upper, dashed line); redrawn from Mannapperuma
area). Data are adapted from Kader (1997) and reprinted et al. (1989). The shaded area represents atmospheres
with permission from Beaudry (1999). Copyright 1999 by observed in commercial MA packages of mixed lettuce-
Elsevier Science. based salads (Cameron et al. 1995). Reprinted with
permission from Beaudry (1999). Copyright 1999 by Elsevier
Science.
44
The composition of the atmosphere within Continuous Films: The movement of O2 and CO2
a package results from the interaction is usually directly proportional to the difference
of a number of factors that include the in gas concentration across the film. Steady-state
permeability characteristics of the package, (constant) O2 and CO2 levels are achieved in the
the respiratory behavior of the plant material, package when the O2 uptake and CO2 production
and the environment. The films making up by the product are equal to that permeating
the package are selected to have specific through the film, a situation that exists only when
permeability characteristics, and changes in the respiratory rate is constant.
these characteristics over time, temperature,
and humidity follow known physical laws. The Perforated Films: The rate of gas movement
environment can be controlled to provide specific through a perforated film is the sum of gas
conditions. In contrast to these known and diffusion through the perforation and gas
controllable factors are the often unknown and permeation through the polymeric film. Generally,
uncontrollable responses of the plant material. total gas flow through the perforations is much
Plant species and cultivar, cultural practices, greater than gas movement through the film. Gas
stage of development, manner of harvest, tissue transmission through microperforations has been
type, and postharvest handling—all of these modeled (Fishman et al. 1996). The rate of gas
factors contribute and influence the response of exchange through perforations in a film is so much
the material to the generated atmosphere. The greater than through continuous films that a 1-mm
scope of plant responses can be further modified perforation in a 0.0025 mm (1 mil) thick low-
by initial gas flush of the package before sealing density polyethylene (LDPE) film has nearly the
and inclusion of chemical treatments to slow same gas flux as a half a square meter area of the
unwanted processes or reduce decay. Each of film. As might be surmised, perforated packages
these components of the packaging process can be are more suitable for produce having a high O2
examined separately to better illustrate how each demand.
contributes to packaging strategies.
Gas Exchange Properties of Continuous and
Perforated Films
Package Parameters
The relative permeability of the package to O2
Atmosphere modification in MAP requires and CO2 differs substantially between continuous
actively respiring plant tissue and a barrier and perforated films and results in considerable
through which gas exchange is restricted. The differences in gas exchange behavior. In packages
reduced O2 and increased CO2 resulting from composed of continuous films, the permeability
tissue respiration create gradients across the film of the package to CO2 is usually 2 to 8 times that
barrier that provide the driving force for gas of O2 permeability. If the rate of O2 uptake by the
movement into and out of the package. The levels product is roughly the same as its production of
of O2 and CO2 within a package depend on the CO2 (the normal case unless fermentation is taking
interaction between commodity respiration and the place), the CO2 gradient will be much lower
permeability properties of the packaging film or than the O2 gradient. For example, low-density
microperforations (Beaudry et al. 1992, Kader et polyethylene (LDPE) has permeability to CO2
al. 1997a). that is four times that of O2. In a LDPE package
having a 10% O2 steady-state atmosphere, the
There are two strategies for creating film. The first CO2 level could be calculated as (21 - 10%)/4,
employs continuous films that control movement or 2.75% CO2. (Air contains 78% N2, 21%
of O2 and CO2 into or out of the package. The O2, and 0.03% CO2.) For perforated films, the
second uses perforated films with small holes, permeability to CO2 is only 0.77 times that of O2.
or microperforations, as the primary route of gas As a result, in a package relying on perforations
exchange.
45
for gas exchange, CO2 levels climb to roughly with CO2, N2, or a combination of gases is often
the same extent that O2 levels decline (that is, the desirable during filling and sealing to rapidly
gradients are nearly equal) such that the sum of obtain the maximum benefits of MAP.
O2 and CO2 concentrations is usually in the range
of 18 to 20%. For any given O2 level, therefore,
the perforated package will have a considerably Respiratory Parameters
higher level of CO2 compared with the continuous
film package. To extend the example above, if a The maximal rate of respiration for most fruit
package were designed to have a 10% O2 steady- and vegetable products undergoes a 4- to 6-fold
state atmosphere using perforations as the route increase from 0 to 15 °C (Beaudry et al. 1992,
of gas exchange, the CO2 level would be 0.77 × Cameron et al. 1994,1995, Lakakul et al. 1999).
(21 - 10%), or 8.8%, which is about 3-fold greater This means that product respiration increases at
than in the continuous film package. The relative 2 or 3 times the rate of LDPE permeability and
elevation of CO2 in perforated film packages may 30 times the rate of perforation permeability
be critically important in package design, if CO2 is with increasing temperature. When respiratory
needed to help control decay or degreening. demand for O2 increases faster than O2 permeation
as temperature increases, O2 levels decline and
MAP relying on a combination of perforation may pose a risk to product quality, limiting the
and permeation has features of both systems, usefulness of MAP in some situations.
with attainable combinations of O2 and CO2 being
between those of packages relying on permeation Safe levels of O2 and CO2 are important for
and those relying on perforations (Mannapperuma package design. A lower O2 limit has been
et al. 1989, Beaudry 1999) (figures 1 and 2). associated with onset of fermentation and
The temperature sensitivity for permeation, and accumulation of ethanol and acetaldehyde
the permeability of O2 and CO2, is somewhere (Beaudry et al. 1992). Fermentation is linked
between those for perforated packages and to the development of off flavors and tissue
continuous film packages. damage. Effect of temperature on lower O2 limit
has been measured for a number of commodities
Temperature is extremely important in package including whole apple, apple slices, blueberry, and
design, and continuous and perforated films raspberry. In each case, lower O2 limit increased
differ in their response to temperature changes. with temperature. Lower O2 limits vary from 0.15
The O2 and CO2 permeability of continuous films to 5% (table 1) and are influenced by temperature,
increases with temperature, while the diffusion commodity, and cultivar (Beaudry and Gran
of gases through perforations is extremely 1993).
insensitive to temperature changes. For instance,
O2 permeation through LDPE can increase 200%
from 0 to 15 °C, but the exchange of O2 through Integrating Package, Product, and
perforations will increase only 11% across this Environment
temperature range.
Mathematical models can integrate the film
Depending on the rates of respiration and permeability to O2, to CO2, and to H2O and the
transmission through the package, atmosphere respiratory response of the commodity to O2 (and
modification can be achieved rapidly or relatively in some cases to CO2), along with its lower O2
slowly. At low temperatures, atmosphere limit and upper CO2 limit (Beaudry et al. 1992,
modification can take several days, such that Cameron et al. 1994, Fishman et al. 1996, Hertog
some package systems would not achieve steady- et al. 1998, Lakakul et al. 1999). These models
state conditions before the end of their shelf-life. enable the reasonably accurate prediction of
In many cases, purging the package atmosphere package performance; that is, O2-, CO2-, and H2O-
46
content in the package headspace under a variety Film thickness and composition with different
of environmental conditions prior to construction permeability characteristics could be selected to
of the package. Additionally, they permit the protect against fermentation.
identification of limiting features of the film,
package design, and product and environment A package model can also be used to clarify the
conditions. Models typically include temperature nature of the mismatch between the temperature
dependency but can also be developed to predict sensitivity of O2 uptake and O2 flux through
effects of package volume, resistance to heat flow, the film and denote methods to ameliorate this
and developmental changes in product physiology problem. One method would be to choose a film
on headspace gases. with permeability changes for O2 similar to that
of the respiration of the product, so if temperature
Predicting and Controlling O2 and CO2 Content increases, respiration and permeability of the film
increase an equivalent amount.
A model can be developed to predict the
steady-state concentration of O2 in the package Another solution to the MAP temperature problem
headspace. Steady-state models incorporating is to develop a package system that senses either
temperature effects on respiration and the environment or the physiological status of the
permeability have been published for many enclosed product and responds by increasing the
commodities. In addition, more complex dynamic permeability to O2 (Cameron et al. 1993). Such
models have been developed to account for “sense-and-respond” packaging is technically
temporal changes in package volume, product difficult to develop, and progress has only been
respiration, and the humidity and temperature of conceptual at this time (Smyth et al. 1999). A third
the environment (Fishman et al. 1996, Hertog et approach is to design packages to function at the
al. 1998). highest temperatures typically encountered in the
distribution and retail cool chain and, as far as
Package performance can be depicted in a number possible, maintain control over the temperature
of ways. Perhaps the most instructive format is of the packaged product, thereby adapting to the
describing the effects of temperature on package limitations imposed by the film. Most companies
O2 levels. For example, a package was designed using MAP have adopted this simple solution.
to produce low O2 and high CO2 levels for 100 Generally, the lowest temperature feasible is
g of apple slices at 0 °C (Lakakul et al. 1999). maintained, since temperature has a much more
The practical O2 limit was set 3-fold higher than significant influence on preserving quality than the
the fermentation threshold to prevent variation application of low O2 (Kays 1997).
in respiration and permeability from causing a
reduction in package O2 below the lower limit. Variation in the respiration rate of the product
If the package relied on perforations for gas and the variation in film or pore permeability can
exchange, it would undergo a rapid decline in influence package design. Variation in product
O2 by the time the package reached 6 °C (43 ºF). respiration and package permeability has been
Films with higher temperature sensitivity would measured for broccoli, and the effect on package
be less prone to risk fermentation. In this example, O2 modeled (Cameron et al. 1993). Cameron et al.
packages were designed to maintain aerobic (1993) concluded that there is an estimable risk of
O2 levels at 15 °C, the highest temperature to the package O2 falling sufficiently low to promote
which they would be exposed. The performance fermentation in any product. Packages should
of the packages can then be predicted at lower be designed to generate O2 levels well above the
temperatures likely to be encountered during lower limit to ensure aerobic conditions.
storage. A package O2 model can also be used
to predict very specific package criteria. For Products such as broccoli, mushrooms, and
instance, the 100 g of apple slices described above leeks have very high rates of respiration, and
were in a container with a film area of 120 cm2. most continuous films do not have the capacity
47
to provide enough O2 to avoid fermentation. Only four perforations were required to achieve
Accordingly, there is commercial interest in near-ambient O2 levels, while 40 perforations only
developing films with high gas transmission rates. reduced RH to 95%
Films that have improved rates of gas transmission
by virtue of their polymeric nature are often Condensation on the inner surface of the film
blends of two or three different polymers, whith is a common problem with MAP. A drop of
each polymer performing a specific function only 0.2 °C in the film temperature can result
such as strength, transparency, and improved gas in condensation in a package with an internal
transmission. Similarly, films can be laminated to RH of 99% at 10 °C. Cold-storage rooms have
achieve needed properties. temperature swings of several degrees, so
condensation is possible in almost any MAP.
The plastic polymer can also be mixed with an Fortunately, there are film surface treatments that
inert inorganic material such as CaCO3 and SiO2 result in droplet dispersion, so the condensing
to generate micoporous films. Gas permeabilities water forms a thin, uniform layer that is virtually
can be manipulated by adjusting the filler invisible. Condensation could also be reduced in
content, particle size of the filler, and degree of MAPs for a few commodities, such as tomato,
stretching. The average pore size ranges from that tolerate low humidity by including materials
0.14 to 1.4 µm in diameter (Mizutani, 1989). in the package that reduce RH. Salts enclosed
Films using microperforations can attain very in permeable sachets can reduce humidity in
high gas transmission rates. The diameter of MAPs of tomato fruit (Shirazi and Cameron
microperforation generally ranges from 40 to 1992). The possibility of using films with very
200 µm; and, by altering the size and thickness high water permeability has been examined using
of microperforations, gas permeability through mathematical models (Cameron et al. 1995).
a package can be altered to meet well-defined However, since the external humidity would
product requirements. Microperforated films have be critical in maintaining the proper package
also been used to extend storage of strawberries RH, this approach would encounter difficulty in
and nectarines (Meyers 1985) and apple (Watkins maintaining a specific humidity level in shipping
et al. 1988). and storage environments.
Plant tissues tend to lose moisture when RH is Product temperature affects storability more than
below 99 to 99.5%. Generally, water loss results in any other factor. Precooling and temperature
visible wilting or wrinkling of the surface of most maintenance during handling and shipping are
commodities when it exceeds 4 to 6% of total critical in preserving quality. Temperature also
fresh weight (Kays 1997). Fortunately, most MAP significantly affects film permeability and thereby
films are relatively impermeable to water. The the O2 and CO2 content of the package. The
RH is very near saturation in most continuous or elevated rate of respiration at high temperature
perforated film packages. A saturated atmosphere could be used to rapidly establish the desired
at 20 ºC (68 ºF) has only 2.1% H2O, and most package atmosphere, but this would only be useful
external environments are at 30 to 60% RH, in the few situations in which it would be more
yielding a water vapor gradient of about 1%. important to rapidly establish the atmosphere than
The O2 gradient can be several-fold higher. to slow physiological processes—for example,
Because of the small driving force and the rapid to reduce cut-surface browning. Cameron et al.
rate of release of water vapor from the product, (1995) calculated that, at 25 ºC (77 ºF), a package
perforations have a much greater effect on the O2 of blueberry fruit could attain a steady-state
level than on RH. Fishman et al. (1996) calculated atmosphere in less than 2 days, whereas it required
that perforating a continuous film increased O2 approximately 20 days at 0 °C (32 ºF).
flow 40-fold more than it increased H2O flow.
48
The temperature of the produce in the package is some cases is partly mediated by ethylene, as with
managed by circulating cool air around the outside broccoli (Ku and Wills 1999).
of the package. The film and the headspace
atmosphere are barriers to heat movement, prevent While low O2 and elevated CO2 atmospheres are
rapid cooling, and reduce the effectiveness of commonly used to minimize ethylene-dependent
refrigeration. A “safe radius” for the distance from responses attendant to ripening in CA rooms,
the center of the package to the circulated air can this goal may not be fully compatible with MAP.
be calculated based on the heat of respiration The problem with the incorporation of MAP for
and the rate at which heat can be removed by ripening control is not one of efficacy, but rather
the cooler air (Sharp et al. 1993). For instance, one of logistics. Modified atmospheres are most
the center of a package of broccoli must have a effective at reducing ripening prior to the onset
radius of less than 14 cm to keep it within 1 °C of of ripening, rather than at a later stage. However,
the refrigerated air. Slower-respiring pear would packaged products are usually intended for
function as well with a larger package having an immediate consumption and an unripe product
effective radius of 50 cm. is not immediately edible or is of lesser quality
than the ripe product. Thus, the advantage of
improved shelf-life by retarding ripening runs
Plant Responses to MAP counter to the needs of the consumer when retail
MAP systems are used. Nevertheless, MAP can
Some of the most important factors that affect reduce the rate of ripening of some commodities
shelf-life of fresh horticultural products are such as tomato even during its later stages (Yang
ripening and senescence, decay, and cut-surface and Chinnan 1988). More potential for using MAP
browning. The effect of MA on these factors has to control ripening may exist at the packinghouse
been well characterized. The application of MA or distributor level, as in the case of overseas
to affect these limiting factors can be restricted shipment of apples (Watkins et al. 1998), rather
for some crops by adverse or nonbeneficial than at the retail level.
physiological responses; for example, induction
of fermentation. Fresh product quality can be Decay
maximized more effectively by good temperature
management than by atmosphere modification. Decay control is a particularly important problem
for many crops. Levels of >10% CO2 effectively
Ripening and Senescence slow or stop the growth of numerous decay
organisms (Brown 1922). Low O2 has a very
Low O2 and elevated CO2 can significantly reduce limited effect on decay organism activity or
the rates of ripening and senescence primarily survival at levels above the fermentation threshold
by reducing the synthesis and perception of of most commodities. While not all horticultural
ethylene (Burg and Burg 1967, Abeles et al. commodities can withstand CO2 levels sufficient
1992). Changes in respiration and starch, sugars, to inhibit fungal activity, a number of highly
chlorophyll, and cell wall constituents during perishable commodities are not adversely affected
ripening and/or senescence can be reduced and, (table 2). Notable among these are strawberry,
in some cases, nearly arrested by eliminating blueberry, blackberry, raspberry, and cherry,
ethylene action through the use of low O2/high which can be stored successfully under a CO2
CO2 atmospheres. atmosphere of 10 to 20%.
Chlorophyll loss, a desirable trait for many Packaging strategies to enhance CO2 include
climacteric fruits, results in quality loss for many initial purging with high levels of CO2. This
vegetables. Chlorophyll degradation during the strategy relies on continued respiration to replace
senescence of green vegetables can be inhibited CO2 lost from the package and is in commercial
by low O2 and elevated CO2, a response that in use for many berry crops. The choice of film type
49
markedly alters the CO2 content of a package. In the development of off flavors as well as physical
particular, perforated and continuous films differ injury (Kays 1997, Mattheis and Fellman 2000).
in their discrimination between O2 and CO2.
Perforated films will generate a higher partial Production of compounds that contribute to
pressure of CO2 for a given concentration of O2 in the characteristic aromas of many fruit, such as
the package. Perforated packages can accumulate apples, bananas, pears, peaches, and strawberries,
CO2 to levels within the fungistatic range. For can be adversely affected by low O2 and elevated
example, a perforated package that generates 1% CO2 (Song et al. 1998, Mattheis and Fellman
O2 could accumulate a 15% CO2 atmosphere. 2000). Synthesis of aroma compounds are
generally suppressed by high CO2 and low O2, in
While high RH reduces water loss, it also part by their action on ethylene perception but also
aggravates decay development. Strategies to via action of O2 on oxidative processes, including
reduce humidity in packages using salt sachets for respiration required for substrate production. Most
the purpose of limiting decay have been explored products that suffered moderate suppression of
(Shirazi and Cameron 1992). A number of aroma volatile production due to low-O2 storage
effective chemical additives can be used at various atmospheres will eventually develop characteristic
points during processing and packaging to reduce flavors. However, low-O2 MAP may suppress
decay. aroma production so consumers perceive reduced
quality upon opening the container.
Cut-Surface Browning
Beaudry, R.M. 1999. Effect of O2 and CO2 partial Fishman, S., V. Rodov, and S. Ben-Yehoshua.
pressure on selected phenomena affecting fruit 1996. Mathematical model for perforation effect
and vegetable quality. Postharvest Biol. Technol. on oxygen and water vapor dynamics in modified-
15:293-303. atmosphere packages. J. Food Sci. 61:956-961.
Beaudry, R.M., A.C. Cameron, A. Shirazi, and Gorny, J.R. 1997. A summary of CA and MA
D.L. Dostal-Lange. 1992. Modified-atmosphere requirements and recommendations for fresh-cut
packaging of blueberry fruit: effect of temperature (minimally-processed) fruits and vegetables. In
on package O2 and CO2. J. Amer. Soc. Hort. Sci. J. Gorny, ed., Fresh-Cut Fruits and Vegetables
117:436-441. and MAP, CA’97 Proceedings, vol. 5, pp. 30-
66. Postharvest Hort. Ser. no. 19, University of
Beaudry, R.M., and C.D. Gran. 1993. Using California, Davis, CA.
a modified-atmosphere packaging approach
to answer some postharvest questions: factors Herner, R.C. 1987. High CO2 effects on plant
affecting the lower oxygen limit. Acta Hort. organs. In J. Weichman, ed., Postharvest
362:203-212. Physiology of Vegetables, pp. 239-253. Marcel
Dekker, New York, NY.
Brown, W. 1922. On the germination and growth
of fungi at various temperatures and in various Hertog, M.L.A.T.M., H.W. Peppelenbos, R.G.
concentrations of oxygen and carbon dioxide. Evelo, and L.M.M. Tijskens. 1998. A dynamic
Ann. Bot. 36:257-283. and generic model of gas exchange of respiring
produce: the effects of oxygen, carbon dioxide and
Burg, S.P., and E.A. Burg. 1967. Molecular temperature. Postharvest Biol. Technol. 14:335-
requirements for the biological activity of 349.
ethylene. Plant Physiol. 42: 114-152.
51
Kader, A.A. 1997a. A summary of CA Mattheis, J.P., and J.P. Fellman. 2000. Impact of
requirements and recommendations for fruits other modified atmosphere packaging and controlled
than apples and pears. In A. Kader, ed., Fruits atmosphere on aroma, flavor and quality of
Other Than Apples and Pears, CA’97 Proceedings, horticultural produce. HortTechnology 10:507-
vol. 2, pp. 1-36. Postharvest Hort. Ser. no. 17, 510.
University of California, Davis, CA.
Meyers, R.A. 1985. Modified atmosphere
Kader, A.A. 1997b. Biological bases of O2 and packaging and process. U.S. Patent 4515266.
CO2 effects on postharvest life of horticultural
perishables. In M.E. Saltveit, ed., Vegetables and Mizutani, Y. 1989. Microporous polypropylene
Ornamentals, CA’97 Proceedings, vol. 4, pp. 160- sheets. Ind. Eng. Chem. Res. 32:221-227.
163. Postharvest Hort. Ser. no. 18, University of
California, Davis, CA. Peiser, G., G. Lopez-Galvez, and M. Cantwell.
1997. Changes in off-odor volatiles of salad
Kader, A.A., D. Zagory, and E.L. Kerbel. 1989. products during storage. In J. Gorny, ed., Fresh-
Modified atmosphere packaging of fruits and Cut Fruits and Vegetables and MAP, CA’97
vegetables. Crit. Rev. Food Sci. 28:1-30. Proceedings, vol. 5, pp. 23-28. Postharvest Hort.
Ser. no. 19, University of California, Davis, CA.
Kays, S.J. 1997. Postharvest Physiology of
Perishable Plant Products, Van Nostrand Reinhold, Reid, M.S. 1997. A summary of CA and
New York, NY. MA requirements and recommendations for
ornamentals and cut flowers. In M.E. Saltveit, ed.,
Ku, V.V.V., and R.B.H. Wills. 1999. Effect of Vegetables and Ornamentals, CA’97 Proceedings,
1-methylcyclopropene on the storage-life of vol. 4, pp. 129-136. Postharvest Hort. Ser. no. 18,
broccoli. Postharvest Biol. Technol. 17:127-132. University of California, Davis, CA.
Lakakul, R., R.M. Beaudry, and R.J. Hernandez. Saltveit, M.E. 1997. A summary of CA and MA
1999. Modeling respiration of apple slices in recommendations for harvested vegetables. In
modified-atmosphere packages. J. Food Sci. M.E. Saltveit, ed., Vegetables and Ornamentals,
64:105-110. CA’97 Proceedings, vol. 4, pp. 98-117.
Postharvest Hort. Ser. no. 18, University of
Mannapperuma, J., D. Zagory, R.P. Singh, and California, Davis, CA.
A.A. Kader. 1989. Design of polymeric packages
for modified atmosphere storage of fresh produce. Sharp, A.K., A.R. Irving, and S.C. Morris. 1993.
In J.K. Fellman, ed., Proceedings of the 5th Does temperature variation limit the use of MA
International Controlled Atmosphere Research packaging during shipment in freight containers?
Conference, Other Commodities and Storage In G. Blanpied, J. Bartsch, and J. Hicks, eds.,
Recommendations, Wenatchee, WA, Vol. 2, pp. Proceedings of the 6th International Controlled
225-233. Atmosphere Research Conference, pp. 238-251.
Cornell University, Ithaca, NY.
52
Shirazi, A., and A.C. Cameron. 1992. Controlling
relative humidity in modified-atmosphere
packages of tomato fruit. HortScience 27:336-339.
53
Wholesale Distribution Center nonfruit vegetables and temperate fruits and
Storage melons. If there is enough capacity in the facility,
the fruits should be stored separately from the
vegetables. This allows installing equipment to
James F. Thompson and Adel A. Kader
maintain higher RH (90 to 98%) for the vegetables
as many of them are quite susceptible to water
Thompson is formerly with the Biological and
loss and wilting. Table 2 shows cut flowers and
Agricultural Engineering Department, University
nursery items divided into the recommended three
of California, Davis, CA. He is now retired. Kader
categories or groups. If handled with produce, the
was with the Department of Pomology, University
floral items in group 1 should be in the group 1A
of California, Davis, CA. He is deceased.
vegetable room to minimize exposure to ethylene
produced by many fruits.
Most produce is shipped from the point of
production to regional or local distributors, such
as terminal markets, independent wholesalers, or
chain store distribution centers. Produce orders
are assembled at these sites and then shipped to
retail stores, restaurants, or institutions such as
schools or hospitals. Produce and floral items
lose quality during these marketing steps, and the
amount of quality loss accumulates at each step.
The consumer will receive quality produce only
if each operation in the handling chain minimizes
abuse caused by mechanical damage, improper
temperature and RH, moisture loss, ethylene
damage, odor contamination, and excessive
storage time.
54
Table 1. Compatible fresh fruits and vegetables during 7-day storage*
Groups 1A and 1B Group 2 Group 3
0-2 °C; group 1A: 90-98% RH; group 1B: 85-95% RH 7-10 °C; 85-95% RH 13-18 °C; 85-95% RH
Group 1A
Vegetables Alfalfa sprouts Chinese cabbage† Mint† Basil† Bitter melon
Amaranth† Chinese turnip Mushroom Beans, snap, green, wax Boniato†
Anise† Collard† Mustard greens† Cactus leaves (nopales) † Cassava
Artichoke Corn, sweet, baby Parsley† Calabaza Dry onion
Arugula† Cut vegetables Parsnip Chayote† Ginger
Asparagus† Daikon† Radicchio Cowpea (Southern pea) Jicama
Beans, fava, lima Endive†-chicory Radish Cucumber† Potato
Bean sprouts Escarole† Rutabaga Eggplant† Pumpkin
Beet Fennel† Rhubarb Kiwano (horned melon) Squash, winter (hard rind) †
Belgian endive† Garlic Salsify Long bean Sweet potato†
Bok choy† Green onion† Scorzonera Malanga† Taro (dasheen)
Broccoli† Herbs† (not basil) Shallot† Okra† Tomato, ripe, partially ripe,
& mature green
Broccoflower† Horseradish Snow pea† Pepper, bell, chili Yam†
Brussels sprouts† Jerusalem artichoke Spinach† Squash, summer (soft rind) †
Cabbage† Kailon† Sweet pea† Tomatillo
Carrot† Kale† Swiss chard† Winged bean
Cauliflower† Kohlrabi Turnip
Celeriac Leek† Turnip greens†
Celery† Lettuce† Water chestnut
Chard† Watercress†
55
56
Table 1. Compatible fresh fruits and vegetables during 7-day storage*—Continued
Groups 1A and 1B Group 2 Group 3
0-2 °C; group 1A: 90-98% RH; group 1B: 85-95% RH 7-10 °C; 85-95% RH 13-18 °C; 85-95% RH
Group 1B
Fruits and Apple‡ Elderberry Prune† Avocado, unripe‡ Lime† Atemoya‡ Sapodilla‡
melons Apricot‡ Fig Quince† Babaco Limequat Banana‡ Sapote‡
Avocado, ripe‡ Gooseberry Raspberry Cactus pear, tuna Mandarin Breadfruit‡ Soursop‡
Barbados cherry Grape Strawberry Calamondin Mango, ripe‡ Canistel‡ Watermelon
Blackberry Kiwifruit†‡ Carambola Olive Casaba melon
Blueberry Loganberry Cranberry Orange Cherimoya‡
Boysenberry Longan Custard apple‡ Passion fruit Crenshaw melon‡
Caimito Loquat Durian, ripe‡ Pepino Honeydew melon‡
Cantaloup‡ Lychee Feijoa Pineapple Jaboticaba
Cashew apple Nectarine Granadilla‡ Pummelo Jackfruit‡
Cherry Peach Grapefruit† Sugar apple Mamey‡
Coconut Pear (Asian & European) Guava‡ Tamarillo Mangosteen‡
Currant Persimmon† Juan canary melon‡ Tamarind Papaya‡
Fresh-cut fruits‡ Plum, ripe† Kumquat Tangelo Persian melon‡
Date Plumcot, ripe† Lemon† Tangerine Plantain‡
Dewberry Pomegranate Ugli fruit Rambutan
Source: Thompson et al. 1996
*Ethylene should be kept below 1 µL L-1 (1 ppm) in the storage area.
†
Sensitive to ethylene damage.
‡
Produces significant ethylene.
Table 2. Compatible flowers, florist’s foliage, and nursery items during 7-day storage†
Group 1* Group 2 Group 3
0-2 °C; 85-95% RH 7-10 °C; 85-95% RH 13-18°C; 85-95% RH
Flowers Acacia Gaillardia Protea Anemone African violet
Alstroemeria Gardenia Rannunculas Bird of paradise Anthurium
Allium Gerbera Rose Camellia Ginger
Aster Gladiolus Snapdragon Eucharis Heliconia
Bouvardia Gypsophlia Snowdrop Gloriosa Orchid, cattleya, vandal
Buddleia Heather Squill Godetia Poinsettia
Calendula Hyacinth Statice Sweet-william Bulbs, corms, rhizomes,
Candytuft Iris Stephanotis tubers, & roots
Carnation Laceflower Stevia Nursery stock
Chrysanthemum Lilac Stock
Clarkia Lily Strawflower
Columbine Lily-of-the-valley Sweet pea
Coreopsis Lupine Tulip
Cornflower Marigolds Violet
Cosmos Mignonette Zinnia
Crocus Narcissus Cuttings &
scions
Dahlia Orchid,
cymbidium
Daisy, English, Ornithogalum
Marguerite,
Shasta
Delphinium Poppy
Feverfew Peony
Forget-me-not Phlox
Foxglove Primrose
Freesia
57
The two warmer temperature ranges in tables 1 containers on the bottom layers of a pallet load
and 2 are for chilling-sensitive produce (groups can minimize some of this damage. Plastic foam
2 and 3). The highest-temperature room can also and returnable plastic containers are often stronger
be used to ripen fruit that only require a warm than typical fiberboard boxes and can reduce
environment to ripen. If refrigerated space is mechanical damage.
limited, low-temperature fruits, vegetables, and
flowers can be mixed in a room; air-conditioned Most distribution facilities have special ripening
space at 20 to 25 °C (68 to 77 °F) can be used for rooms or areas reserved for fruit ripening.
highest-temperature products (group 3). Ripening rooms are used extensively for bananas
and may also be used to ripen avocados, kiwifruit,
Many green vegetables and most floral products mangoes, tomatoes, nectarines, peaches, plums,
are quite sensitive to ethylene damage. Ethylene and European pears. Pressurized or forced-air
must be kept away from these products. Minimize ripening rooms allow better control of ripening
ethylene from nearby banana-ripening rooms by— compared with older methods of space-stacking
• using ethylene levels of 100 µL L-1 in the boxes in a warm room. The new designs force
ripening rooms instead of the higher levels temperature-controlled air through the boxes to
often used in commercial operations, maintain fairly uniform product temperature.
• venting ripening rooms to the outside after Ethylene gas (100 to 150 µL L-1) is added to the
the exposure period is complete and before atmosphere on a schedule appropriate for each
rooms are opened, product, and CO2 levels are kept below 1% by
• ventilating the area around ripening rooms ventilating the rooms with outside air. Ripening
at least once a day or install an ethylene is done with air temperature in the range of 15
scrubber, and to 25 °C (59 to 77 °F), and water vapor is added
• using battery-powered forklifts instead to the air to keep RH above 85 to 95% in order
of internal combustion-driven units (for to reduce moisture loss. The ripening of some
example, propane-powered units). products, like stone fruit and pears that were
treated with ethylene at the packing operation, can
Floral products are particularly sensitive to be promoted by warming them to 13 to 18 °C (55
ethylene. Some distribution facilities have found to 64 °F).
that the previously described precautions are
inadequate in preventing damage to flowers. They
have chosen to handle flowers with dairy or meat Reference
products, where ethylene is low, or they require
that all floral products be chemically treated to Thompson, J., A.A. Kader, and K. Sylva. 1996.
resist ethylene damage. Compatibility chart for fruits and vegetables in
short-term transport and storage. Nat. Res. Pub.
Weak fiberboard containers are usually the cause no. 21560, University of California, Division of
of mechanical damage to produce between Agriculture and Natural Resources, Oakland, CA.
packing and retail display. If products arrive at the
distribution facility in crushed boxes, store buyers
must work with suppliers to use stronger boxes or
ensure that packed boxes are correctly stacked and
palletized.
58
Grocery Store Display Storage fruits and vegetables, because ethylene production
and action at this temperature range are minimal.
Ornamentals that are chilling-sensitive and
Adel A. Kader and James F. Thompson
ethylene-sensitive should be handled in a separate
area from the ethylene-producing fruits of group 3
Kader was with the Department of Plant
to avoid ethylene damage.
Sciences, University of California, Davis, CA.
He is deceased. Thompson is formerly with
All produce items should be near their optimum
the Biological and Agricultural Engineering
storage temperature when received at the grocery
Department, University of California, Davis, CA.
store and should be unloaded and moved quickly
He is now retired.
to their appropriate storage area. Keeping
cold commodities at warmer temperatures for
more than a few minutes can result in water
Introduction
condensation on the commodity, which may
encourage the growth of decay-producing
Fresh produce received at the grocery store is kept
pathogens. RH should be kept within the optimum
in storage rooms and display areas (in cabinets
range of 85 to 95% for most commodities to
and cases or on racks and tables) for a few hours
minimize water loss. Good air circulation within
to a few days before purchase by consumers or
the storage room is essential to maintain proper
removal by produce personnel. During this time,
product temperature and RH. Thus, space for
the key factors in maintaining quality are careful
air movement should be kept around stacks or
handling to minimize mechanical injuries, storage
pallets of boxes and between them and the room
and display within optimum ranges of temperature
walls. Enough fresh, ethylene-free air should be
and RH, and proper sanitation of storage and
introduced into storage rooms to keep ethylene <1
display areas. Expedited handling and effective
µL L-1 (<1 ppm), and preferably <0.1 µL L-1 (<0.1
rotation of the produce (first in, first out) is also
ppm) if it can be done economically using fresh
recommended.
air exchanges and/or ethylene scrubbing systems.
Display Fixtures
Storage Room
Most produce items in groups 1 and 2 should be
The number and size of storage rooms depend
displayed in refrigerated display cases. Display
on store size and frequency of produce delivery
at store ambient air temperature is acceptable for
to the grocery store. If three rooms are available
some commodities, including produce that does
for produce, they are best designated for short-
not lose water quickly and has a long shelf-life
term storage of the three groups of fruits and
like apple, pear, kiwifruit, and orange. Produce
vegetables mentioned in the chapter Wholesale
that is on sale (special promotion) or that will
Distribution Center Storage; that is, group 1 at 0
be on display for a few hours (like grapes and
to 2 ºC (32 to 36 °F), group 2 at 7 to 10 °C (45
strawberries) can also be stored at ambient
to 50 °F), and group 3 at 13 to 18 ºC (55 to 64
temperatures.
°F). If only two rooms are available, one should
be used for group 1 at 0 to 2 ºC (32 to 36 °F) and
Ideally, the display case temperature range should
the other for groups 2 and 3 (at a compromise
match the recommended range for each group of
temperature range of 10 to 14 ºC [50 to 57 °F]).
commodities: 0 to 2 ºC (32 to 36 °F) for group
If only one room is available, it should be kept
1, including all fresh-cut products, and 7 to 10
at a compromise temperature of 5 ºC (41 °F) and
°C (45 to 50 °F) for group 2. Since display cases
used for groups 1 and 2, while group 3 should
usually do not have the refrigeration capacity to
be kept in an air-conditioned area. Cut flowers
cool the products, it is important to ensure that
and other ornamentals that are best kept at 0 to 2
the product is near its recommended temperature
°C (32 to 36 °F) can be combined with group 1
59
when it is placed in the display case. The produce (R.W. Daniels, Audits International, personal
should not obstruct the discharge air and return air communication, 1989). A survey of temperatures
outlets to maintain good cold air circulation within of fresh-cut vegetable products kept in refrigerated
the case. Also, produce should not be stacked display cases in some grocery stores indicates an
so densely that cold air circulation is blocked or overall mean of about 5 ºC (41 °F) with more than
so high that it is out of the refrigerated zone and 40% of the products having temperature above
becomes exposed to ambient air temperatures. about 7 ºC (45 °F) (Jeff Leshuk, Sensitech Inc.,
personal communication, 1989). This indicates
Refrigerated display cases have either a significant improvements in maintaining the
horizontal or a vertical air flow system and either cold chain within the grocery stores, but more
single-tier or multi-tier display shelves. They improvements are needed to bring the temperature
should be equipped with easy-to-read, accurate range for fresh-cut products close to the
thermometers, which should be calibrated and recommended 0 to 2 °C (32 to 36 °F).
monitored regularly. Performance of refrigerated
display cases is influenced primarily by their Water-loss reduction can be achieved by
refrigeration capacity, defrost options, and air protecting produce from excessive air movement;
circulation system. Important secondary factors packaging in perforated polymeric films
include temperature, RH, and movement of (as moisture barriers); periodically adding
surrounding air and radiant heat from the lighting sanitized, clean water by misting (only useful for
sources. commodities that tolerate wetting, such as those
listed in table 1); and/or displaying on crushed
A 1989 survey of temperatures of fresh-cut ice (only useful for products that tolerate direct
salads kept in refrigerated display cases in a contact with ice). If ice is used, proper drainage
representative sample of grocery stores indicated of the melt water should be provided. It should
an overall mean temperature of about 9 ºC (48 be remembered that ice is not an effective way to
ºF), with more than 78% having temperatures keep the product cold unless it is well surrounded
above about 7 ºC (45 °F) and more than 17.5% by the ice.
having temperature above about 13 ºC (55 °F)
Table 1. Produce that benefit from misting while displayed in refrigerated cases
______________________________________________________________________________
Artichoke Corn, sweet Peppers
Asparagus* Eggplant Radishes
Beans, snap Endive Rhubarb
Beets Kale Shallots, green
Broccoli Leeks Spinach
Brussels sprouts Lettuce Sprouts
Cabbage Mustard greens Squash, summer
Carrots Onions, green Swiss chard
Cauliflower Parsley Turnips
Celery Parsnips Watercress
Collards Peas
______________________________________________________________________________
*Asparagus should be displayed vertically with cut ends on a wet absorbent pad.
60
Non-refrigerated display tables or racks are used Thompson, J.F., and A.A. Kader. 2009. From the
for most group 3 fruits and vegetables, which Farm to Your Table, A Consumer’s Guide to Fresh
should be displayed separately. Some of the fruits Fruits and Vegetables. Publication no. 21643,
in groups 1 and 2, such as avocado, kiwifruit, and Department of Agriculture and Natural Resources,
pear, may be displayed on non-refrigerated display University of California, Oakland, CA. At
tables or racks at ambient produce department http://anrcatalog.ucdavis.edu/Items/21643.aspx
temperatures to enhance their ripening. Daylight-
simulating fluorescent bulbs can provide adequate
lighting in the produce department without giving
off heat.
Further Reading
61
Chilling and Freezing Injury Both temperature and duration of exposure are
involved in the development of chilling injury.
Chien Yi Wang Damage may occur in a short time if temperatures
are considerably below the threshold level, but a
Wang is formerly with the Food Quality product may be able to withstand temperatures
Laboratory, Henry A. Wallace Beltsville a few degrees into the critical zone for a longer
Agricultural Research Center, Agricultural time before injury becomes irreversible. Maturity
Research Service, U.S. Department of Agriculture, at harvest and degree of ripeness are important
Beltsville, MD. He is now retired. factors in determining chilling sensitivity in some
fruits like avocados (Kosiyachinda and Young
1976), honeydew melons (Lipton 1978), and
Chilling Injury tomatoes (McColloch et al. 1966). The effects of
chilling are cumulative in some commodities. Low
Many fruits, vegetables, and ornamentals of temperatures in transit, or even in the field shortly
tropical or subtropical origin are sensitive to low before harvest, add to the total effects of chilling
temperatures (Paull 1990). These crops are injured that occur in cold storage.
after a period of exposure to chilling temperatures
below 10 to 15 °C (50 to 59 °F) but above their Treatments shown to alleviate chilling injury
freezing points (Lyons 1973, Wang 1990). Certain include intermittent warming; high- or low-
horticultural crops of temperate origin are also temperature preconditioning; CA storage;
susceptible to chilling injury (Bramlage and Meir pretreatments with ethylene, abscisic acid, methyl
1990). Those temperate crops, in general, have jasmonate, and other natural compounds; calcium
lower threshold temperatures of <5 °C (41 °F). or other chemical applications; hypobaric storage;
At these chilling temperatures, the tissues weaken waxing; film packaging; and genetic manipulation
because they are unable to carry on normal (Ryall and Lipton 1979, Wang 1993, 1994, Meir et
metabolic processes. Various physiological and al. 1996).
biochemical alterations and cellular dysfunctions
occur in chilling-sensitive species in response Chilling injury is discussed more specifically
to chilling stress (Wang 1982, Wang and Adams under each commodity. Many of the commodities
1982, Raison and Orr 1990). When chilling stress susceptible to chilling injury are listed in table
is prolonged, these alterations and dysfunctions 1 with threshold temperatures and some of the
will lead to the development of a variety of symptoms.
chilling injury symptoms such as surface lesions,
internal discoloration, water-soaking of the tissue,
and failure to ripen normally (Saltveit and Morris
1990). Often, products that are chilled will still
look sound when remaining in low temperatures.
However, symptoms of chilling injury become
evident shortly after they are moved to warmer
temperatures. Fruits and vegetables that have
been chilled may be particularly susceptible to
decay. Weak pathogens such as Alternaria spp.,
which do not grow readily on healthy tissues,
can attack tissues that have been weakened by
low-temperature exposure (McColloch and
Worthington 1952, McColloch 1962).
62
Table 1. Fresh produce susceptible to chilling injury when stored at low but
nonfreezing temperatures
__________________________________________________________________
Lowest Safe Temperature Symptoms of injury when stored
Commodity º C º F between 0 ºC and safe temperature*
__________________________________________________________________________
Apples—certain
cultivars 2-3† 36-38 Internal browning, brown core, soggy
breakdown, soft scald
Asparagus 0-2 32-36 Dull, gray-green, limp tips
Atemoya 4 39 Skin darkening, failure to ripen, pulp
discoloration
Avocados 4.5-13 40-55 †
Grayish-brown discoloration of flesh
Bael 3 38 Brown spots on skin
Bananas 11.5-13† 53-56 Dull color when ripened
Bean (lima) 1-4.5 34-40 Rusty brown specks, spots or areas
Bean (snap) 7† 45 Pitting and russeting
Breadfruit 7-12 45-53 Abnormal ripening, dull brown
discoloration
Choyote 5-10 41-50 Dull brown discoloration, pitting, flesh
darkening
Cranberries 2 36 Rubbery texture, red flesh
Cucumbers 7 45 Pitting, water-soaked spots, decay
Eggplants 7 45 Surface scald, alternaria rot, blackening of
seeds
Ginger 7 45 Softening, tissue breakdown, decay
Guavas 4.5† 40 Pulp injury, decay
†
Grapefruit 10 50 Scald, pitting, watery breakdown
Jicama 13-18 55-65 Surface decay, discoloration
Lemons 11-13† 52-55 Pitting, membranous staining, red blotch
Limes 7-9 45-48 Pitting, turning tan with time
Lychee 3 38 Skin browning
†
Mangos 10-13 50-55 Grayish scald-like discoloration of skin,
uneven ripening
Mangosteen 4-8 39-47 Hardening and browning of the cortex
Melons
Cantaloupe 2-5† 36-41 Pitting, surface decay
Honeydew 7-10 45-50 Reddish-tan discoloration, pitting, surface
decay, failure to ripen
Casaba 7-10 45-50 Pitting, surface decay, failure to ripen
Crenshaw and
Persian 7-10 45-50 Pitting, surface decay, failure to ripen
Okra 7 45 Discoloration, water-soaked areas, pitting,
decay
Olive, fresh 7 45 Internal browning
Oranges 3† 38 Pitting, brown stain
Papayas 7 45 Pitting, failure to ripen, off flavors, decay
63
Table 1. Fresh produce susceptible to chilling injury when stored at low but
nonfreezing temperatures—Continued
__________________________________________________________________
Lowest Safe Temperature Symptoms of injury when stored
Commodity º C º F between 0 ºC and safe temperature*
__________________________________________________________________________
Passion fruit 10 50 Dark red discoloration on skin, loss of
flavor, decay
Peppers, sweet 7 45 Sheet pitting, alternaria rot on pods and
calyxes, darkening of seeds
Pineapples 7-10† 45-50 Dull green when ripe, internal browning
Pomegranates 4.5 40 Pitting, external and internal browning
†
Potatoes 3 38 Mahogany browning, sweetening
Pumpkins and
hardshell squash 10 50 Decay, especially alternaria rot
Rambutan 10 50 Darkening of exocarp
Sweet potatoes 13 55 Decay, pitting, internal discoloration,
hardcore when cooked
Tamarillos 3-4 37-40 Surface pitting, discoloration
Taro 10 50 Internal browning, decay
Tomatoes
Ripe 7-10† 45-50 Water soaking and softening, decay
Mature-green 13 55 Poor color when ripe, alternaria rot
Water convolvulus 10 50 Darkening of leaves and stems
Watermelons 4.5 40 Pitting, objectionable flavor
___________________________________________________________________________
*Symptoms often become apparent only after removal to warm temperatures, as in marketing.
†
See individual commodity sections in this Handbook.
64
Freezing Injury The susceptibility of different fresh fruits and
vegetables to freezing injury varies widely. Some
The recommended storage temperatures for commodities may be frozen and thawed a number
commodities that are not susceptible to chilling of times with little or no injury, whereas others are
injury are as low as possible but slightly above permanently injured by even a slight freezing. All
the freezing point. Freezing injury occurs fruits and vegetables can be categorized into three
when ice crystals form in the tissues. Cultivars, groups based on their sensitivity to freezing: most
locations, and growing conditions may affect susceptible—those that are likely to be injured by
the freezing point. To be on the safe side, the even one light freezing, moderately susceptible—
highest temperature at which freezing of a those that will recover from one or two light
specific commodity may occur should be used as freezing periods, and least susceptible—those that
a guide for recommending the optimum storage can be lightly frozen several times without serious
temperature. More detailed discussion of freezing damage. Table 2 shows the relative susceptibility
points and factors affecting them can be found of a number of fruits and vegetables to freezing
in McColloch (1953), Whiteman (1957), and injury.
Parsons and Day (1970,1971). The most common
symptom of freezing injury is a water-soaked
appearance. Tissues injured by freezing generally
lose rigidity and become mushy upon thawing.
65
The freezing point of the commodity is no (Lutz 1936). Even though a number of fruits and
indication of the damage to be expected by vegetables are somewhat tolerant to freezing,
freezing. For example, both tomatoes and parsnips commodities recovered from freezing often have
have freezing points of -1.1 to -0.6 °C (30 to 31 shorter storage life and are more susceptible
°F), but parsnips can be frozen and thawed several to invasion by microorganisms. For example,
times without apparent injury, whereas tomatoes apples that recover from freezing are softer than
are ruined after only one freezing. The severity normal fruit, and carrots that have been frozen
of freezing injury is influenced by a combination are especially subject to decay. Therefore, it is
of time and temperature. For example, apples best to avoid subjecting fresh produce to freezing
that would be injured little by exposure to temperatures in the first place.
temperatures slightly below the freezing point
for a few days would be severely injured by just
a few hours of exposure to -7 to -10 °C (19 to References
14 °F). The susceptibility to freezing injury is
not necessarily similar for the same type of fruit Bramlage, W.J., and S. Meir. 1990. Chilling injury
or vegetable. For example, leafy lettuce is very of crops of temperate origin. In C.Y. Wang, ed.,
susceptible to freezing injury, whereas some other Chilling Injury of Horticultural Crops, pp. 37-49.
leafy vegetables, such as kale and cabbage, can CRC Press, Boca Raton, FL.
withstand several light freezing periods without
serious injury. Hruschka, H.W., R.V. Akeley, and E.H. Ralph.
1961. Seed potato productivity after cooling,
When left undisturbed, most fruits and vegetables supercooling or freezing. Marketing Research
can usually be cooled one to several degrees Report 507, U.S. Department of Agriculture,
below their freezing point before they actually Washington, DC.
freeze. This cooling without freezing is known
as undercooling or supercooling. They may Kosiyachinda, S., and R.E. Young. 1976. Chilling
remain undercooled for several hours, but sensitivity of avocado fruit at different stages of
they will usually start to freeze immediately if the respiratory climacteric. J. Amer. Soc. Hort.
jarred or moved. If permitted to warm above Sci. 101:665-667.
the freezing point, many commodities that have
been undercooled may escape having ice crystals Lipton, W.J. 1978. Chilling injury of ‘Honey Dew’
form in them. For example, potatoes, which are muskmelons: symptoms and relation to degree of
very sensitive to freezing damage, showed no ripeness at harvest. HortScience 13:45-46.
freezing symptoms from having been undercooled
for a short time to -4 °C (25 °F), about 3 °C (5 Lutz, J.M. 1936. The influence of rate of thawing
°F) below their freezing point, when they were on freezing injury of apples, potatoes and onions.
carefully warmed after undercooling (Hruschka et Proc. Amer. Soc. Hort. Sci. 33:227-233.
al. 1961).
Lyons, J.M. 1973. Chilling injury in plants. Ann.
Plant tissues are very sensitive to bruising while Rev. Plant Physiol. 24:445-466.
frozen, and this sensitivity is another reason for
leaving commodities undisturbed until they have McColloch, L.P. 1953. Injuries from chilling
warmed. Selecting a suitable thawing temperature and freezing. In Plant Diseases, the Yearbook of
involves a compromise. Fast thawing damages Agriculture 1953, pp. 826-830. U.S. Department
tissues, but very slow thawing such as at 0 to 1 °C of Agriculture, Washington, DC.
(32 to 34 °F) allows ice to remain in the tissues
too long and causes injury. Research on the rate
of thawing has suggested that thawing at 4 °C (39
°F) causes the least damage for most commodities
66
McColloch, L.P. 1962. Alternaria rot following Saltveit, M.E., and L.L. Morris. 1990. Overview
chilling injury of acorn squashes. Marketing on chilling injury of horticultural crops. In C.Y.
Research Report 518, U.S. Department of Wang, ed., Chilling Injury of Horticultural Crops,
Agriculture, Washington, DC. pp. 3-15. CRC Press, Boca Raton, FL.
McColloch, L.P., and J.T. Worthington. 1952. Wang, C.Y. 1982. Physiological and biochemical
Low temperature as a factor in the susceptibility responses of plants to chilling stress. HortScience
of mature-green tomatoes to Alternaria rot. 17:173-186.
Phytopathology 42:425-427.
Wang, C.Y. 1990. Chilling Injury of Horticultural
McColloch, L.P., J.N. Yeatman, and P. Loyd. Crops. CRC Press, Boca Raton, FL.
1966. Color changes and chilling injury of pink
tomatoes held at various temperatures. Marketing Wang, C.Y. 1993. Approaches to reduce chilling
Research Report 735, U.S. Department of injury of fruits and vegetables. Hort. Rev. 15:63-
Agriculture, Washington, DC. 95.
Meir, S., S. Philosoph-Hadas, S. Lurie, et al. 1996. Wang, C.Y. 1994. Reduction of chilling injury by
Reduction of chilling injury in stored avocado, methyl jasmonate. Acta. Hort. 368:901-907.
grapefruit, and bell pepper by methyl jasmonate.
Can. J. Bot. 74:870-874. Wang, C.Y., and D.O. Adams. 1982. Chilling-
induced ethylene production in cucumbers
Parsons, C.S., and R.H. Day. 1970. Freezing (Cucumis sativus L). Plant Physiol. 69:424-427.
injury of root crops: Beets, carrots, parsnips,
radishes, and turnips. Marketing Research Report Whiteman, T.M. 1957. Freezing points of fruits,
866, U.S. Department of Agriculture, Washington, vegetables and florist stocks. Marketing Research
DC. Report 196, U.S. Department of Agriculture,
Washington, DC.
Parsons, C.S., and R.H. Day. 1971. Freezing
injury to bell peppers. Marketing Research Report
895, U.S. Department of Agriculture, Washington,
DC.
67
Respiratory Metabolism There is little the postharvest physiologist can do
to alter the internal factors affecting respiration
Mikal E. Saltveit of harvested commodities, because they are
largely a function of the commodity itself once
Saltveit is with Mann Laboratory, Department of harvested. However, a major part of postharvest
Vegetable Crops, University of California, Davis, technology is devoted to reducing respiration
CA. and other metabolic reactions associated with
quality retention by manipulating the external
environment.
Introduction
In general, the storage life of commodities varies
All of the commodities covered in this handbook inversely with the rate of respiration. This is
are alive and carry on processes characteristic of because respiration supplies compounds that
all living things. One of the most important of determine the rate of metabolic processes directly
these is respiratory metabolism. The process of related to quality parameters such as firmness,
respiration involves combining O2 in the air with sugar content, aroma, and flavor. Commodities
organic molecules in the tissue (usually a sugar) and cultivars with higher rates of respiration
to form various intermediate compounds and tend to have shorter storage life than those with
eventually CO2 and water. Energy produced by lower rates of respiration. Storage life of broccoli,
the series of reactions making up respiration can lettuce, peas, spinach, and sweet corn, all of which
be captured as high-energy bonds in compounds have high respiration rates, is short in comparison
used by the cell in subsequent reactions, or it can to that of apples, cranberries, limes, onions, and
be lost as heat. The energy and organic molecules potatoes, all of which have low respiration rates
produced during respiration are used by other (table 1).
metabolic processes to maintain the health of the
commodity. Heat produced during respiration
is called “vital heat,” and it contributes to the
refrigeration load that must be considered in
designing storage rooms.
68
Factors Affecting Respiration commodity (table 2). This table shows that, if a
commodity has a mean shelf-life of 13 days at 20
Respiration is affected by a wide range of °C (68 °F), it can be stored for as long as 100 days
environmental factors that include light, chemical at 0 °C (32 °F) but will last no more than 4 days at
stress (for example, fumigants), radiation stress, 40 °C (104 °F).
water stress, growth regulators, and pathogen
attack. The most important postharvest factors Table 2. Effect of temperature on rate of
are temperature, atmospheric composition, and deterioration
physical stress. ________________________________________
Temperature Assumed Relative Relative
Temperature. The most important factor Q10 velocity of shelf-life
affecting postharvest life is temperature, because deterioration
temperature has a profound effect on the rates of ________________________________________
biological reactions; for example, metabolism °C
and respiration. Over the physiological range of 0 — 1.0 100
most crops, 0 to 30 °C (32 to 86 °F), increased 10 3.0 3.0 33
temperatures cause an exponential rise in 20 2.5 7.5 13
respiration. The Van’t Hoff Rule states that the 30 2.0 15.0 7
velocity of a biological reaction increases 2 to 40 1.5 22.5 4
3-fold for every 10 °C (18 °F) rise in temperature. ________________________________________
The temperature quotient for a 10 °C (18 Chilling stress. Although respiration is normally
°F) interval is called the Q10. The Q10 can be reduced at low but nonfreezing temperatures,
calculated by dividing the reaction rate at a certain commodities, chiefly those originating
higher temperature by the rate at a 10 °C (18 °F) in the tropics and subtropics, exhibit abnormal
lower temperature: Q10 = R2/R1. The temperature respiration when their temperature falls below
quotient is useful because it allows us to calculate 10 to 12 °C (50 to 54 ºF). Typically, the Q10
the respiration rates at one temperature from a is much higher at those low temperatures for
known rate at another temperature. However, the chilling-sensitive crops than it would be for
respiration rate does not follow ideal behavior, and chilling-tolerant ones. Respiration may increase
the Q10 can vary considerably with temperature. dramatically at the chilling temperatures or
At higher temperatures, the Q10 is usually smaller when the commodity is returned to nonchilling
than that at lower temperatures. temperatures. This enhanced respiration
presumably reflects the cells’ efforts to detoxify
The following are typical values for Q10 at various metabolic intermediates that accumulated during
temperature ranges: chilling, as well as to repair damage to membranes
and other subcellular structures. Enhanced
Temperature Q10 respiration is only one of many symptoms that
0 to 10 °C 2.5 to 4.0 signal the onset of chilling injury.
10 to 20 °C 2.0 to 2.5
20 to 30 °C 1.5 to 2.0 Heat stress. As the temperature rises beyond
30 to 40 °C 1.0 to 1.5 the physiological range, the rate of increase in
respiration falls. It becomes negative as the tissue
These typical Q10 values allow us to construct a nears its thermal death point, when metabolism
table showing the effect of different temperatures is disorderly and enzyme proteins are denatured.
Many tissues can tolerate high temperatures for
on the rates of respiration or deterioration
short periods of time (for example, minutes),
and relative shelf-life of a typical perishable
and this property is used to advantage in killing
69
surface fungi on some fruits. Continued exposure physiological changes in adjacent, non-wounded
to high temperature results in phytotoxic tissue. Some of the more important changes
symptoms and then complete tissue collapse. include enhanced respiration, ethylene production,
However, conditioning and heat shocks— phenolic metabolism, and wound healing.
that is, short exposure to potentially injurious Wound-induced respiration is often transitory,
temperatures—can modify the tissue’s responses lasting a few hours or days. However, in some
to subsequent harmful stresses. tissues, wounding stimulates developmental
changes, such as promotion of ripening, that
Atmospheric composition. Adequate O2 levels result in a prolonged increase in respiration.
are required to maintain aerobic respiration. The Ethylene stimulates respiration and stress-induced
exact level of O2 that reduces respiration while ethylene may have many physiological effects on
still permitting aerobic respiration varies among commodities besides stimulating respiration.
commodities. In most crops, O2 levels at around
2% to 3% produce a beneficial reduction in the Stage of development. Respiration rates vary
rate of respiration and other metabolic reactions. among and within commodities. Storage organs
Levels as low as 1% improve the storage life of such as nuts and tubers have low respiration
some crops—for example, apples—but only when rates. Tissues with vegetative or floral meristems
the storage temperature is optimal. At higher such as asparagus and broccoli have very high
storage temperatures, the demand for adenosine respiration rates. As plant organs mature, their rate
triphosphate (ATP) may outstrip the supply and of respiration typically declines. This means that
promote anaerobic respiration (see chapters commodities harvested during active growth, such
“Controlled Atmosphere Storage” and “Modified as many vegetables and immature fruits, have high
Atmosphere Packaging”). The need for adequate respiration rates. Mature fruits, dormant buds, and
O2 should be considered in selecting the various storage organs have relatively low rates.
postharvest handling procedures, such as waxing
and other surface coatings, film wrapping, and After harvest, the respiration rate typically
packaging. Unintentional modification of the declines—slowly in nonclimacteric fruits and
atmosphere, by packaging for example, can result storage organs and rapidly in vegetative tissues
in production of undesirable fermentative products and immature fruits. The rapid decline presumably
and development of foul odors. reflects depletion of respirable substrates, which
are typically low in such tissues. An important
Increasing the CO2 level around some exception to the general decline in respiration
commodities reduces respiration, delays following harvest is the rapid and sometimes
senescence, and retards fungal growth. In low dramatic rise in respiration during the ripening of
O2 environments, however, increased CO2 levels climacteric fruit (figure 1). This rise, which has
can promote fermentative metabolism. Some been the subject of intense study for many years,
commodities tolerate brief storage in a pure N2 normally consists of four distinct phases: (1)
atmosphere (for example, a few days at low preclimacteric minimum, (2) climacteric rise, (3)
temperatures) or in very high concentrations of
climacteric peak, and (4) postclimacteric decline.
CO2. The biochemical basis for this ability to
withstand these atmospheres is unknown.
70
Significance of Respiration
The division of fruits into climacteric and Loss of substrate. Use of various substrates in
nonclimacteric types has been very useful for respiration can result in loss of food reserves in
postharvest physiologists. However, some fruits, the tissue and loss of taste quality (especially
kiwifruit and cucumber for example, appear sweetness) and food value to the consumer.
to blur the distinction between the groups. For certain commodities that are stored for
Respiratory rises also occur during stress and extended periods of time, such as onions used for
other developmental stages, but a true climacteric dehydrated product, the loss of dry weight due
only occurs coincident with fruit ripening. The to respiration can be significant. When a hexose
following is a general classification of fruits sugar (for example, glucose) is the substrate, 180
according to their respiratory behavior during g of sugar is lost for each 264 g of CO2 produced
by the commodity. The rate of dry weight loss can
ripening:
be estimated as follows:
Climacteric Fruits Nonclimacteric Fruits
Dry weight loss (g kg-1 h-1) =
Apple Papaya Blueberry
Respiration (mg CO2 kg-1 h-1) × 0.068
Apricot Passion fruit Cacao
or
Avocado Peach Caju % dry weight loss (g 100 g-1 h-1) =
Banana Pear Cherry Respiration (mg CO2 kg-1 h-1) × 68 × 10-6
Biriba Persimmon Cucumber
Breadfruit Plum Grape For example, onions held at 30 °C (86 °F)
Cherimoya Sapote Grapefruit will respire at about 35 mg CO2 kg-1 h-1. The
Feijoa Soursop Lemon percentage dry weight loss per hour would be
Fig Tomato Lime 35 x 0.68/10,000 = 0.0024%, while the percentage
Guava Watermelon Olive dry weight loss per month would be 0.0024 x 24 x
Jackfruit Orange 30 = 1.73%.
Kiwifruit Pepper
Mango Pineapple Synthesis of new compounds. Postharvest
Muskmelon Strawberry storage can be used either to prevent any
Nectarine Tamarillo reduction in quality or to promote changes that
increase quality. The quality of most vegetables
(for example, cucumbers and lettuce) and
nonclimacteric fruit (for example, strawberries)
is maximal at harvest, and storage conditions are
71
optimized to prevent quality loss. In contrast, Some commodities have high respiration rates
many flowers (for example, carnations and and require considerably more refrigeration than
roses), nonclimacteric fruit (for example, more slowly respiring produce to keep them at a
lemons and oranges), and climacteric fruit (for specified temperature. For example, asparagus,
example, bananas and tomatoes) are harvested broccoli, mushrooms, and peas respire about 10
before they reach their best quality, and storage times faster than apples, cabbage, lemons, and
conditions are optimized to permit development tomatoes.
of optimum quality. In the first case, the synthesis
of new compounds is unnecessary because they Meaning of the respiratory quotient (RQ).
lead to reduced quality (for example, enzymes The composition of a commodity frequently
that destroy chlorophyll in lettuce or promote determines which substrates are used in respiration
lignification in asparagus). In the second case, and consequently the value of the respiratory
synthesis of pigments and volatiles (for example, quotient (RQ). RQ is defined as the ratio of CO2
lycopene in tomatoes and amyl esters in banana), produced to O2 consumed; CO2 and O2 can be
loss of chlorophyll (for example, chlorophyll- measured in moles or volumes. Depending on
degrading enzymes in banana and lemons), and the substrate being oxidized, RQ values for fresh
the conversion of starch to sugar (for example, commodities range from 0.7 to 1.3 for aerobic
sweetening of apples and bananas) is necessary for respiration. When carbohydrates are being
development of maximum quality. These synthetic aerobically respired, RQ is near 1, while it is <1
reactions require energy and organic molecules for lipids and >1 for organic acids. Very high RQ
derived from respiration. values usually indicate anaerobic respiration in
those tissues that produce ethanol. In such tissues,
Release of heat energy. The heat produced by a rapid change in RQ can be used as an indication
respiration (vital heat), about 673 kcal for each of the shift from aerobic to anaerobic respiration.
mole of sugar (180 g) used, can be a major factor
in establishing the refrigeration requirements
during transport and storage. Vital heat must Measuring the Rate of Respiration
be considered in selecting proper methods
for cooling, package design, and stacking of The rate of any reaction can be determined
packages, as well as selection of refrigerated by measuring the rate at which the substrates
storage facilities (that is, refrigeration capacity, disappear or the products appear. Apart from the
air circulation, and ventilation). The approximate water produced by respiration, which is relatively
rates of heat production by various crops at trivial compared with the very high water content
different storage temperatures can be calculated of most harvested commodities, all the substrates
from the respiration rates for many fruits and and products of respiration have been used to
vegetables given in section “Summary of determine the rate of respiration. They are loss
Respiration and Ethylene Production Rates” in the of substrate (for example, glucose) loss of O2,
Introduction of this Handbook. increase in CO2, and production of heat. The most
commonly used method is to measure production
Calculation of heat production from the respiration of CO2 with either a static or a dynamic system.
equation shows that production of 1 mg of CO2
yields 2.55 cal. In the language of the refrigeration In a static system, the commodity is enclosed in an
engineer, a respiration rate of 1 mg CO2 kg-1 h-1 airtight container and gas samples are taken after
indicates heat production of 61.2 kcal tonne-1 day1 sufficient CO2 has accumulated to be accurately
(220 BTU ton-1 day-1). The British thermal unit detected by any one of a number of commercially
(BTU) is the heat required to raise 1 lb of water by available instruments (for example, gas
1 °F. chromatograph and infrared CO2 analyzer). If the
container is properly sealed, CO2 should increase
linearly with time. Multiplying the change in
72
concentration times the container volume and C6H12O6 + 6 O2 → 6 CO2 + 6 H2O + 686 kcal mole-1
dividing by weight of the commodity and duration
of time between samples gives the production rate. The components of this reaction have various
sources and destinations. The one mole of glucose
In the dynamic system a flow of air (or other (180 g) can come from stored simple sugars like
gas mixture) is passed through the container glucose and sucrose or complex polysaccharides
at a known rate. The system will come into like starch. Fats and proteins can also provide
equilibrium (>99.3%) in about the same time it substrates for respiration, but their derivatives
takes for 5 times the volume to flow through the (fatty acids, glycerol, and amino acids) enter at
container. The difference in CO2 concentration later stages in the overall process and as smaller,
between the inlet and outlet is measured after partially metabolized molecules. The 192 g of
the system has reached equilibrium by taking O2 (6 moles × 32 g mol-1) used to oxidize the
gas samples at both points and analyzing them. 1 mole of glucose diffuses into the tissue from
Multiplying the difference in concentration by the surrounding atmosphere, while the 6 moles
the flow rate and dividing by the weight of the of CO2 (264 g) diffuses out of the tissue. The 6
commodity calculates the production rate. moles of H2O (108 g) that are produced are simply
incorporated into the aqueous solution of the cell.
Biochemistry of Respiration There are three fates for the energy (686 kcal mol-1)
released by aerobic respiration. Around 13 kcal is
Respiration is the oxidative breakdown of lost due to the increase in entropy (disorder) when
complex substrate molecules normally present in the complex glucose molecule is broken down
plant cells, such as starches, sugars, and organic into simpler molecules. Of the remaining 673 kcal
acids, to simpler molecules such as CO2 and H2O. that are capable of doing work, around 281 kcal
Concomitant with this catabolic reaction is the (about 41% of the total energy) is used to produce
production of energy and intermediate molecules 38 ATP molecules (38 ATP × 7.4 kcal ATP-1).
that are required to sustain the myriad of The remaining 392 kcal (57%) is lost as heat. In
metabolic reactions essential for the maintenance actuality, most energy is lost as heat since energy is
of cellular organization and membrane integrity lost to heat every time energy is transferred during
of living cells. Since respiration rate is so tightly a metabolic reaction.
coupled to the rate of metabolism, measurements
of respiration provide an easy, nondestructive Aerobic respiration involves a series of three
means of monitoring the metabolic and complex reactions, each of which is catalyzed by
physiological state of tissues. For example, events a number of specific enzymes that perform one of
of senescence and ripening are often signaled by the following actions: add an energy-containing
abrupt changes in respiration. phosphate group to the substrate molecule,
rearrange the molecule, and break down the
Maintaining a supply of high-energy compounds molecule to a simpler one. The three interconnected
like adenosine triphosphate (ATP), nicotinamide metabolic pathways are glycolysis, tricarboxylic
adenine dinucleotide (NADH), and pyrophosphate acid (TCA) cycle, and electron transport system.
(PPi) is a primary function of respiration. The
overall process of aerobic respiration involves Glycolysis, the breakdown, or lysing, of glucose,
regeneration of ATP from ADP (adenosine occurs in the cytoplasm of the cell. It involves the
diphosphate) and Pi (inorganic phosphate) with production of two molecules of pyruvate from
release of CO2 and H2O. If glucose is used as each molecule of glucose. Each of the 10 distinct,
substrate, the overall equation for respiration can sequential reactions in glycolysis is catalyzed by
be written as follows: one enzyme. Two key enzymes in glycolysis are
phosphofructokinase (PFK) and pyruvate kinase
73
(PK). Cells can control their rate of energy of the anaerobic cell and takes place during the
production by altering the rate of glycolysis, reductive decarboxylation of pyruvate to ethanol
primarily through controlling PFK and PK in fermentative metabolism.
activity. One of the products of respiration, ATP,
is used as a negative feedback inhibitor to control Fermentation, or anaerobic respiration, involves
the activity of PFK. Glycolysis produces two the conversion of hexose sugars into alcohol and
molecules of ATP and two molecules of NADH CO2 in the absence of O2. Pyruvate produced
from the breakdown of each molecule of glucose. through glycolysis via a series of reactions that
do not require O2 can be converted to lactic acid,
Tricarboxylic acid (TCA) cycle, which occurs in malic acid, acetyl CoA, or acetaldehyde. The
the mitochondrial matrix, involves the breakdown pathway chosen depends on cellular pH, prior
of pyruvate into CO2 in nine sequential, enzymatic stresses, and the current metabolic needs of the
reactions. Pyruvate is decarboxylated (removal of cell. Acidification of the cytoplasm enhances the
CO2) to form acetate, which condenses with a co- activity of pyruvic decarboxylase that then shunts
enzyme to form acetyl CoA. This compound then pyruvate to form CO2 and acetaldehyde. The
enters the cycle by condensation with oxaloacetate acetaldehyde is converted by the enzyme alcohol
to form citric acid. Citric acid has three dehydrogenase to ethanol with the regeneration
carboxyl groups from which the cycle derives of NAD+. Two molecules of ATP and 21 kcal of
its name. Through a series of seven successive
heat energy are produced in anaerobic respiration
rearrangements, oxidations, and decarboxylations,
(alcoholic fermentation) from each molecule of
citric acid is converted back into oxaloacetate
glucose. To maintain the supply of ATP at the
that is then ready to accept another acetyl CoA
aerobic rate, 19 times as many glucose molecules
molecule. In addition to producing the many small
would be needed, and glycolysis would increase
molecules that are used in the synthetic reactions
of the cell, the TCA cycle also produces one 19-fold. However, since only two molecules of
molecule of flavin adenine dinucleotide (FADH2) CO2 are produced during glycolysis, instead of
and four molecules of NADH for each molecule six during aerobic respiration, the rate of CO2
of pyruvate metabolized. production would not increase by 19-fold but only
by 6.3-fold (that is, 19 ÷ 3). Concomitantly, there
Electron transport system, which occurs on would be substantial accumulation of ethanol
membranes in the mitochondria, involves and smaller amounts of acetaldehyde. However,
the production of ATP from the high-energy glycolysis usually increases only 3- to 6-fold.
intermediates FADH2 and NADH. The energy
contained in a molecule of NADH or FADH2 is The O2 concentration at which a shift from
more than is needed for most cellular processes. predominantly aerobic to predominantly anaerobic
In a series of reactions, one NADH molecule respiration occurs varies among tissues and is
produces three ATP molecules, while one FADH known as the extinction point, the anaerobic
molecule produces two ATP molecules. The compensation point, and the fermentative
production of ATP depends not only on the energy threshold. Since O2 concentration at any point in a
contained in NADH and FADH2 but also on the fruit or vegetable varies with rates of gas diffusion
chemical environment (pH and ion concentrations) and respiration, some parts of the commodity may
within the cell and mitochondria. become anaerobic while others remain aerobic.
74
Further Reading Wills, R.H.H., T.H. Lee, D. Graham, et al. 1981.
Postharvest - An Introduction to the Physiology
Abeles, F.B., P.W. Morgan, and M.E. Saltveit. and Handling of Fruit and Vegetables. AVI
1992. Ethylene in Plant Biology, 2nd ed. Publishing Company, Westport, CT.
Academic Press, New York, NY.
75
Ethylene Effects include not only the commodity’s epidermis but
also postharvest coatings and packaging. Under
Mikal E. Saltveit biotic or abiotic stress or during climacteric
ripening, C2H4 production can increase
Saltveit is with Mann Laboratory, Department of dramatically, and emanations from stimulated
Plant Sciences, University of California, Davis, tissue can accumulate in packages or storerooms
CA. and produce unwanted effects in adjacent tissue.
Other molecules with specific configurations can
mimic C2H4 but are less effective. For example,
Introduction C2H4 analogs propylene (C3H6) and acetylene
(C2H2) require 100- and 2,700-fold, respectively,
Ethylene (C2H4) is a simple, naturally occurring the concentration of C2H4 to elicit the same effect.
organic molecule that is a colorless gas at
biological temperatures. The following is a list of Plants produce C2H4 through an actively
biological attributes of ethylene: regulated biosynthetic pathway in which the
• It is a colorless gas at biological amino acid methionine is converted to ACC
temperatures. (1-aminocyclopropane-1-carboxylic acid) and
• It is a naturally occurring organic compound. then to C2H4 through a series of biochemical
• It readily diffuses from tissue. reactions. O2 is required for the synthesis of C2H4
• It is produced from methionine via and both O2 and CO2 are required for its biological
aminocyclopropane carboxylate (ACC) by a activity. Each reaction in the synthesis and action
highly regulated metabolic pathway. of C2H4 involves a biological catalyst, an enzyme
• Key enzymes are ACC synthase and ACC that focuses the reaction into producing the next
oxidase. specific chemical for that pathway. Enzyme
• C2H4 synthesis is inhibited by C2H4 in activity is regulated either through its synthesis
vegetative and immature reproductive tissue. and/or destruction, or by interactions with
• C2H4 synthesis is promoted (autocatalytic) substrates and products. These interactions can
by C2H4 in mature reproductive climacteric create a positive or a negative feedback of C2H4 on
tissue. its synthesis (figure 1).
• It is effective at ppm and ppb concentrations
(1 ppm = 6.5 x 10-9 M at 25 °C).
Removal of
• It requires O2 to be synthesized, and O2 and
ethylene
low levels of CO2 to be active. Positive
feedback
Ethylene concentration
78
Prior and current stresses have a significant effect Controlling Ethylene Action
on modulating the effect of C2H4. For example,
wounding stimulates C2H4 production, as well There are roughly three ways to control the action
as a host of plant defense responses such as of C2H4 in plants. The first is to prevent the plant
increased phenylpropanoid metabolism. Some of from being exposed to biologically active levels
these responses involve C2H4, while others do not. of C2H4. The second is to prevent the plant tissue
Increased phenolic metabolism greatly increases from perceiving the C2H4 that is in its surrounding
the susceptibility of some crops like lettuce to atmosphere or that is being produced by the tissue.
develop browning—for example, russet spotting— The third is to prevent the plant from responding
when exposed to C2H4 and/or mechanical injury. to the perceived C2H4 by controlling exposure to
C2H4.
The effect of tissue susceptibility is most clearly
seen in fruit tissue. Immature climacteric fruit Preventing Exposure to Ethylene
respond to C2H4 with increased respiration and
reduced C2H4 production. Once the tissue has The following should be done to prevent exposure
reached a certain stage of maturity, however, C2H4 to C2H4:
not only promotes increased respiration but also • Keep the air around the commodity C2H4
increased C2H4 synthesis. free.
• Use fresh, C2H4-free air from outside.
Controlling the effectiveness of C2H4 does not • Scrub C2H4 from the storage atmosphere.
always involve a reduction in its activity. There • Use sachets of C2H4 absorbers inside
are many beneficial effects of C2H4 that can be packages to reduce levels.
enhanced (see above). The techniques used to • Segregate C2H4-producing commodities
increase the effectiveness of C2H4 are almost the from C2H4-sensitive ones.
mirror image of techniques used to reduce its • Keep exposure to a minimum (in terms of
effectiveness. both duration and level).
• Inhibit C2H4 synthesis (AVG, ACC synthase,
Ethylene action can be enhanced by using low O2, ACC oxidase).
cultivars that are sensitive and respond uniformly
to C2H4 rather than cultivars that are C2H4 Risk of exposure to C2H4 is usually not much of
insensitive. An effective concentration of C2H4 a problem in the field because the levels of C2H4
should be maintained around the tissue for a found even in polluted air rarely reach biologically
sufficient time to elicit the full response. However, active levels. However, in greenhouses, cold-
since the response to C2H4 is log-linear (a log storage rooms, and transportation vehicles, C2H4
increase in C2H4 concentration results in a linear can frequently accumulate to reach biologically
increase in the response), there is an extremely active levels. Ethylene found in these enclosed
large range over which the concentrations are spaces comes from varied sources, and the two
effective. The application of C2H4 must be at most prominent sources are diseased, stressed,
the proper stage of development and at the or ripening plant tissue and the incomplete
proper temperature for the desired effects to be combustion of organic fuels.
induced. Ethephon and similar C2H4-releasing
chemicals permit the commercial application of With proper ventilation of enclosed spaces and
C2H4 in the field. After harvest, C2H4 gas, either with persistent attention to the condition of
from compressed gas cylinders or catalytically adjacent plants and the operation of heaters and
generated from alcohol, can be used in enclosed gas-powered forklifts, C2H4 can be kept below
storage rooms. biologically active levels. Sometimes the C2H4
that we are concerned with comes from the
79
plant itself. Application of inhibitors of C2H4 Preventing Response by the Plant
biosynthesis, such as AVG and AOA, to the tissue
before or after harvest can significantly reduce The third way to control C2H4 is to prevent the
this source of C2H4 exposure. For example, tissue plant from responding to the perceived C2H4. This
can be prevented from making either stress or can be done by interfering with the metabolic
autocatalytic C2H4 by blocking the biosynthetic machinery that is induced by exposure to C2H4, by
pathway for C2H4 synthesis. If exposure cannot be methods such as the following:
prevented or has already occurred, then both the • Store at coldest possible temperature.
duration of exposure and the level of C2H4 in the • Store under CA or MA or in MAP.
atmosphere should be kept as low as possible. • Inhibit or reduce specific enzyme
activities using chemical inhibitors (for
Preventing Perception of Ethylene example, AIP) or genetic engineering (for
example, antisense or other gene knockout
If significant amounts of C2H4 are in the techniques).
immediate environment, certain methods can be • Divert protein synthesis—by heat-shock, for
used to block the perception of C2H4 by the plant. example.
Here are some possible methods: • Minimize time before use (for example,
• Store at the coldest possible temperature. consumption).
• Use inhibitor of C2H4 perception: CO2,
silver (for example, silver thiosulfate), and Since all the effects of C2H4 on plants that we
1-methyl cyclopropene (1-MCP). are interested in involve metabolic changes,
• Use C2H4-insensitive cultivars. reducing the rate of metabolism by lowering
• Interrupt the C2H4-induced signal. the temperature, withholding a vital reactant
(for example, O2), or by inhibiting a specific
Since perception is a metabolic process, holding enzyme (for example, with a chemical or through
the tissue at the lowest possible temperature will genetic engineering) will prevent a response to
effectively reduce perception. Specific chemical C2H4. For example, ripening promoted by C2H4
inhibitors can also be used that directly interfere often entails tissue softening that significantly
with the perception event. reduces shelf-life. Using antisense technology to
reduce the activity of enzymes involved in tissue
A gaseous inhibitor like CO2 or 1-MCP can be softening has produced fruit that remain firmer
introduced into the atmosphere. The tissue can be longer. Ethylene also promotes phenylpropanoid
dipped or fed a nonvolatile inhibitor such as silver metabolism in many tissues that use stress-
thiosulfate, but this treatment is limited to nonfood produced C2H4 as a signal to induce defense
crops. Ethylene-resistant cultivars can be selected mechanisms. Interfering with synthesis or activity
or the tissue genetically engineered to lack the of phenylalanine ammonia lyase (PAL, the first
necessary biochemical receptors for ethylene or enzyme in phenolic metabolism) with chemical
the signal pathway necessary to transduce the inhibitors or heat treatment eliminates tissue
signal into a physiological event. response to C2H4, preventing development of
postharvest disorders.
Even after the molecular perception event has
occurred, blocking the transduced signal will
effectively prevent perception. However, effective
methods to do this will require a far greater
understanding of the signal pathway than is
currently available.
80
Application of Ethylene pathogen growth. Judicious maintenance of proper
ripening environments will ensure production
The quality of some fruits is increased when they of high-quality fruit. Care must be exercised in
are harvested at a mature but unripe stage that venting and opening ripening rooms to prevent
can withstand the rigors and duration of transport release of sufficient amounts of C2H4 to adversely
and then treated with C2H4 to promote ripening affect other commodities stored in the same
before sale. These fruit include avocados, bananas, warehouse.
honeydew melons, lemons, oranges, and tomatoes.
Treatment with C2H4 stimulates many metabolic Saltveit, M.E. 1999. Effect of ethylene on quality
pathways, including respiration. Oxygen use is of fresh fruits and vegetables. Postharv. Biol.
increased, as is the production of CO2 and heat. Technol. 15:279-292.
Rooms designed to hold produce being exposed
to C2H4 must be designed with extra air-moving
capacity to ensure that an optimal ripening Further Reading
environment is maintained around the crop.
Exposure to C2H4 must be uniform throughout Abeles, F.B., P.W. Morgan, and M.E. Saltveit.
the room and within packages. Heat of respiration 1992. Ethylene in Plant Biology, 2nd ed.
and excessive CO2 must be removed to maintain Academic Press, New York, NY.
a proper environment. Loss of water by the crop
will be increased by the rise in respiratory heat Kader, A.A. 1985. Ethylene-induced senescence
production. Maintaining a high RH can lessen and physiological disorders in harvested
water loss, but too much water vapor can decrease horticultural crops. HortScience 20:54-57.
the strength of cardboard boxes and promote
81
Lelievre, J.M., A. Latche, B. Jones, et al. 1997.
Ethylene and fruit ripening. Physiol. Plant.
101:727-739.
82
1-Methylcyclopropene (1-MCP) phase. A process has been developed in which
1-MCP is complexed with α-cyclodextrin,
Chris B. Watkins maintaining the stability of 1-MCP. After
application, 1-MCP is released from the complex
Department of Horticulture, Cornell University, to expose horticultural products to the molecule.
Ithaca, NY
When applied during the preharvest period,
1-MCP has the useful effects of delaying fruit
Introduction drop, slowing fruit maturation and ripening, and
maintaining postharvest quality (McArtney et
The plant hormone ethylene affects a wide range al. 2008, Watkins 2010, Watkins et al. 2010).
of physiological processes in horticultural crops, Produce must be exposed to 1-MCP at an effective
including abscission, senescence and ripening, rate and for a sufficiently long time to elicit
chlorophyll loss, softening, physiological physiological responses. In essence, this means
disorders, sprouting, isocoumarin synthesis, applying a higher preharvest concentration of
lignification, discoloration (browning), decay, 1-MCP than that used for postharvest application,
and stimulation of defense systems (Saltveit while ensuring stability of the formulation without
1999). Depending on the desired use of the inducing phytotoxicity.
produce, these effects can be positive or negative.
However, most postharvest handling is focused Some reports on postharvest dipping of fruit into
on controlling ethylene production or action. aqueous 1-MCP have been published (Choi et
Among the available methods, chemical control of al. 2008), but this method is not currently used
ethylene biosynthesis by aminoethoxyvinylglycine commercially.
(AVG) and inhibition of its action by
1-methylcyclopropene (1-MCP) have become
useful tools for the horticulture industry as they Registration
seek to maintain quality of produce after harvest
(Venburg et al. 2008, Watkins 2008c). The U.S. Environmental Protection Agency
(EPA) approved use of 1-MCP on floriculture
1-MCP belongs to a class of compounds known as and ornamental products in 1999 and on edible
cyclopropenes. The discovery that cyclopropenes food products in 2002. By 2011, more than 40
inhibit ethylene perception by competitively countries had approved use of 1-MCP. It is
binding to ethylene receptors represented a major registered for use on a wide variety of fruits and
breakthrough in controlling ethylene responses vegetables including apple, apricot, Asian pear,
of horticultural products (Blankenship and Dole avocado, banana, broccoli, calabrese, cauliflower,
2003). The process of discovery of the effects Brussels sprouts, cabbage, carrot, cherimoya,
of cyclopropenes and their proposed method of cucumber, date, guava, kiwifruit, lime, mango,
action has been described (Sisler and Serek 2003, melon, nectarine, papaya, paprika, peach, pear,
Sisler 2006). pepper, persimmon, pineapple, plantain, plum,
plumcot, squash, tomato, and many ornamentals.
1-MCP has several characteristics that make it The specific products for which 1-MCP is
conveniently useful by the fresh produce industry. registered in each country vary greatly according
It is a gaseous molecule that is easily applied, has to the importance of the crop in that country. For
an excellent safety profile, leaves no residues in example, 1-MCP can be applied to tulip bulbs in
or on treated produce, and is active at very low the Netherlands.
concentrations (parts per billion).
Recently, 1-MCP formulations have been
Of the cyclopropenes, 1-MCP proved to be approved by the EPA and other regulatory
extremely active, but it is unstable in the liquid authorities for preharvest application.
83
Semicommercial trials have been carried out at • Nonclimacteric fruit can also respond
several locations in the United States, Argentina, to 1-MCP; such effects are providing
Brazil, Canada, Chile, New Zealand, and South interesting insights about ethylene-dependent
Africa. and ethylene-independent events during
ripening.
• Treated fruit are firmer, slower to soften,
Effects of 1-MCP on Fruits, Vegetables, slower to change peel color and they develop
and Ornamental Products aroma and flavor slower, but if 1-MCP
concentrations and exposure periods are
The availability of 1-MCP has provided appropriate for the product, the final quality
outstanding opportunities for researchers attained in the ripened fruit is similar to that
to investigate both ethylene-dependent and of untreated product.
ethylene-independent events during ripening and • Rate of loss of nutritionally important
senescence, in addition to developing practical compounds such as vitamin C are
uses for 1-MCP. Studies have focused on the usually reduced in 1-MCP-treated fruits
effects of 1-MCP on quality of horticultural
and vegetables, and effects on phenolic
crops, specific postharvest issues such as handling
compounds are minor.
and packaging, physiological and biochemical
responses, and storage disorders. A number of
Physiological Disorders. An important area
detailed reviews on the effects of 1-MCP have
of postharvest responses to 1-MCP is its
been published (Blankenship and Dole 2003,
effects on physiological disorders (Watkins
Serek et al. 2006, Watkins 2006,2007, Huber
2007,2008a,2008b). These disorders can be
2008, Watkins 2008a,2008b,2010). These reviews
divided into categories:
report on the use of an extensive range in 1-MCP
• Ethylene-induced disorders. Examples
concentration, depending on the responsiveness of
include russet spotting of lettuce and
the product to the molecule.
isocoumarin accumulation in carrots (Fan
Ripening and Senescence. 1-MCP affects many and Mattheis 2000a), lignification of
ripening and senescence processes, including asparagus (Liu and Jiang 2006), and water-
pigments, softening and cell wall metabolism, soaking of watermelons (Mao et al. 2004).
flavor and aroma, and nutritional properties These disorders are preventable by inhibition
(Watkins 2006,2008b, Serek et al. 2006). These of ethylene perception.
processes are affected to varying degrees in both • Senescence-related disorders. Examples
nonclimacteric and climacteric products. The include senescent breakdown of apples
range of responses reflects the enormous diversity (Moran and McManus 2005), senescent
of these crops in terms of both inherent diversity scald and breakdown of pears (Ekman et
and morphological derivation (Huber 2008). al. 2004), and yellowing of broccoli (Fan
and Mattheis 2000b). These disorders are
Several generalizations can be made about also prevented by inhibition of ethylene
responses of crops to 1-MCP: perception.
• Genotype, cultivar, and maturity effects can • Controlled atmosphere-related storage
be highly variable, but responses to 1-MCP disorders. 1-MCP can increase susceptibility
are typically “concentration × exposure of apple fruit to carbon dioxide injuries.
time” dependent. Incidence of both internal and external forms
• Most if not all climacteric fruit are affected of injury is increased by 1-MCP (DeEll et
by 1-MCP treatment, but the capacity to al. 2003, Fawbush et al. 2008, Argenta et al.
interrupt the progression of ripening, once 2010).
initiated, varies by fruit and by attributes
studied.
84
• Chilling-related disorders that are increased The apple has been an excellent crop for use of
by inhibition of ethylene perception. 1-MCP, which is used extensively around the
Examples include woolliness and internal world to maintain quality through the whole
breakdown of peaches and nectarines (Dong marketing chain from storage to consumer
et al. 2001), chilling injury of citrus and (Watkins 2008b). Applications rates for apples
bananas (Porat et al. 1999, Jiang et al. 2004), vary from 625 to 1,000 nL L-1, depending on
and flesh browning of the ‘Empire’ apple the country of registration. The major benefit of
(Watkins 2008b). 1-MCP to the grower is more time to get high
• Chilling-related disorders that are decreased quality produce through marketing channels to
by inhibition of ethylene perception. the consumer. The successful use of 1-MCP on
Examples include superficial scald; brown apples is largely associated with varieties for
core (coreflush) and soft scald of apples which maintenance of at-harvest quality and only
and pears (Fan et al. 1999); internal flesh moderate softening to a crisp texture is desirable.
browning of avocados and pineapples
(Selvarajah et al. 2001, Pesis et al. 2002); In contrast, challenges exist for effective use of
1-MCP on fruits that ripen to a melting texture
and chilling injury of bamboo shoots (Luo
or have major color change. For example, failure
et al. 2008), melons (Gal et al. 2006), and
to ripen normally has been shown in avocado,
persimmon (Luo 2007).
banana, pear, and tomato after fruit were treated
at an early ripening stage or if the applied
Pathological Disorders: Disease incidence
1-MCP concentration was too high (Golding
can be increased, decreased, or unaffected by
et al. 1998, Mir et al. 2004, Hurr et al. 2005,
1-MCP, depending on the product, although
Bai et al. 2006). Fruit must ripen uniformly to
results are not always consistent because of the
quality characteristics (texture, flavor, aroma,
complex interaction between host, pathogen,
color) that are expected by the consumer. Despite
and environment (Watkins 2008b). In some
the challenges, successful 1-MCP treatment of
instances, disease incidence can be lower because
avocados, bananas, melons, persimmons, and
the beneficial effects of 1-MCP on skin integrity
tomatoes has resulted from careful attenuation
and flesh firmness result in greater resistance to
of 1-MCP concentrations or selecting fruit at the
infection. However, ethylene is necessary for
appropriate ripening stage at harvest. Research
defense systems in other plant systems (Marcos et
on reinitiating ripening after 1-MCP treatment
al. 2005).
in extremely sensitive fruit like pear is ongoing
(Bai et al. 2006, Chiriboga et al. 2011, Villalobos-
Acuna et al. 2011). A considerable amount of
Application
research on crops other than apple is proprietary
and therefore not yet in the public domain.
For postharvest use of 1-MCP on both ornamental
plants and edible food products, material must
be treated in an enclosed area, such as a storage
References
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Leakage of 1-MCP from the treatment area
can reduce its concentration and therefore Argenta, L.C., J.P. Mattheis, and X.T. Fan.
effectiveness. 1-MCP can only be applied by 2010. Interactive effects of CA storage,
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storage operators. Testing of rooms for leakage, quality of apple fruit. Acta Hort. 857:259-266.
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2006. Re-initiating softening ability of Development of apple superficial scald, soft scald,
1-methylcyclopropene-treated ‘Bartlett’ and core flush, and greasiness is reduced by MCP. J.
‘d’Anjou’ pears after regular air or controlled Agric. Food Chem. 47:3063-3068.
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48:206-214.
Huber, D.J. 2008. Suppression of
DeEll, J.R., D.P. Murr, L. Wiley, and M.D. ethylene responses through application of
Porteous. 2003. 1-Methylcyclopropene (1- 1-methylcyclopropene: a powerful tool for
MCP) increases CO2 injury in apples. Acta Hort. elucidating ripening and senescence mechanisms
600:277-280. in climacteric and nonclimacteric fruits and
vegetables. HortScience 43:106-111.
Dong, L., H.W. Zhou, L. Sonego, et al. 2001.
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of ‘Flavortop’ nectarine. Postharv. Biol. Technol. Differential responses in color changes and
23:105-115. softening of ‘Florida 47’ tomato fruit treated
at green and advanced ripening stages with the
Ekman, J.H., M. Clayton, W.V. Biasi, and E.J. ethylene antagonist 1-methylcyclopropene.
Mitcham. 2004. Interactions between 1-MCP HortTechnology 15:617-622.
concentration, treatment interval and storage
time for ‘Bartlett’ pears. Postharv. Biol. Technol. Jiang, Y.M., D.C. Joyce, W.B. Jiang, and W.J. Lu.
31:127-136. 2004. Effects of chilling temperatures on ethylene
binding by banana fruit. Plant Growth Regul.
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Reduction of ethylene-induced physiological
disorders of carrots and iceberg lettuce by Liu, Z.Y., and W.B. Jiang. 2006. Lignin deposition
1-methylcyclopropene. HortScience 35:1312- and effect of postharvest treatment on lignification
1314. of green asparagus (Asparagus officinalis L.).
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Fan, X.T., and J.P. Mattheis. 2000b.
Yellowing of broccoli in storage is reduced by
1-methylcyclopropene. HortScience 35:885-887.
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Luo, Z.S. 2007. Effect of 1-methylcyclopropene Porat, R., B. Weiss, L. Cohen, et al. 1999. Effects
on ripening of postharvest persimmon (Diospyros of ethylene and 1-methylcyclopropene on the
kaki L.) fruit. LWT—Food Sci. Technol. 40:285- postharvest qualities of ‘Shamouti’ oranges.
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1-methylcyclopropene for alleviating chilling of fresh fruits and vegetables. Postharv. Biol.
injury and lignification of bamboo shoot Technol. 15:279-292.
(Phyllostachys praecox f. prevernalis) during cold
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Mao, L.C., Y. Karakurt, and D.J. Huber. 2004. is suppressed by a postharvest application of
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Technol. 33:1-9. Controlling ethylene responses in flowers at the
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citrus fruits to Penicillium digitatum infection and receptor level. Biotechnol. Adv. 24:357-367.
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Moran, R.E., and P. McManus. 2005. Firmness Watkins, C.B. 2006. The use of
retention, and prevention of coreline browning 1-methylcyclopropene (1-MCP) on fruits and
and senescence in ‘Macoun’ apples with vegetables. Biotechnol. Adv. 24:389-409.
1-methylcyclopropene. HortScience 40:161-163.
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88
Texture Textural attributes of fruits and vegetables are
related to the structural, physiological, and
Judith A. Abbott and F. Roger Harker biochemical characteristics of the living cells;
their changes over time; and their alteration
Abbott is formerly with the Food Quality by processes such as cooking or freezing. The
Laboratory, Henry A. Wallace Beltsville continuous physiological changes in living cells
Agricultural Research Center, Agricultural plus the inherent variability among individual
Research Service, U.S. Department of Agriculture, units of the commodity make the assessment of
Beltsville, MD. She is now retired. Harker is with fruit or vegetable texture difficult. Because of their
the Mt. Albert Research Centre, Horticulture and continuous change, textural measurements are
Food Research Institute of New Zealand Ltd., often relevant only at the time of evaluation; that
Auckland, New Zealand. is, they usually cannot be used to predict condition
much later in the storage period or marketing
chain.
Introduction
89
Table 1. Examples of fruits and vegetables derived from various plant parts
____________________________________________________________________________________
Plant part Fruit Vegetable Seasoning or garnish
____________________________________________________________________________________
Root Beet, carrot, cassava (yucca, Licorice
Manihot), parsnip, radish,
sweet potato (Ipomoea),
turnip, yam (Dioscorea)
90
Table 1. Examples of fruits and vegetables derived from various plant parts—
Continued
____________________________________________________________________________________
Plant part Fruit Vegetable Seasoning or garnish
____________________________________________________________________________________
Fruit, mature Apple, atemoya, avocado, Breadfruit, tomatillo, tomato, Allspice, caper berries,
blueberry, carambola, winter squashes (pumpkin, juniper, mace, pepper
cherimoya, cherry, citrus, hubbard, acorn, etc.) (red, Capsicum),
cranberry, date, grape, tamarind, vanilla bean
jackfruit, mango, olive,
papaya, peach, pear,
pineapple, pomegranate,
strawberry
Seeds Nuts, inclusions in Beans (mature), coconut, Anise, caraway,
numerous fruits peanuts, sweet corn, nuts, cardamon, cumin,
inclusions in numerous dill seed, fennel,
fruit—type vegetables (for mustard, nutmeg,
examples, squashes, pepper (black, Piper),
tomatoes, and beans) pomegranate, poppy
seed, sesame seed
which separates adjacent cells and is rich in filled spaces. The cells can be arranged either
pectic substances. The unique mixture of matrix as columns or as a staggered array where each
(pectic and hemicellulosic) and fibrous (cellulosic) cell overlays the junction of the two lower
polysaccharides in the cell wall mostly determines cells (Sørensen et al. 1999). These differences
the mechanical properties of these cells. The in cell packing may, in part, explain genotypic
polysaccharides confer on the wall two important differences in susceptibility to harvest splitting
but seemingly incompatible properties. The first in carrot. In apple cortical tissue, the cells are
is the wall’s plasticity, which enables it to expand large (up to 300 μm in diameter), elongated along
as the cell enlarges during plant development. the direction of the fruit radius, and organized
The second is the wall’s rigidity, which confers into distinct columns (Khan and Vincent 1993).
strength and determines cell shape. However, on As a result of this orientation of apple cells, the
its own the cell wall is unable to provide much tissue stiffness (elastic modulus) is higher and
mechanical support. Rather, it is the interaction the strain at failure is lower when tissue plugs are
between rigidity of the wall and internal compressed in a radial rather than a vertical or
hydrostatic pressure (turgor) of cell contents that tangential orientation (Khan and Vincent 1993,
provides support. Abbott and Lu 1996). Up to 25% of the volume of
apple tissue may be gas-filled intercellular spaces,
The arrangement and packing of parenchyma cells which indicates relatively inefficient cell packing
within the tissue is another factor that influences and a low degree of cell-to-cell contact, both
mechanical strength of produce. In carrots, the of which correlate well (negatively) with tissue
cells are small (approximately 50 μm in diameter), stiffness (Vincent 1989).
isodiametric in shape, and closely packed with
a high degree of contact between neighboring
cells and a small volume of intercellular gas-
91
Cell Wall Cooking often results in degradation of pectic
polymers via β-elimination, which is usually
From a chemical perspective, the primary cell related to the degree of methyl esterification of
wall of parenchyma cells is composed of a pectin (Waldron et al. 1997). Along with turgor
mixture of cellulose, hemicellulose, and pectin. loss, this process is responsible for thermal
The specific intermolecular interactions among softening. However, some vegetables either don’t
these polysaccharides are poorly understood but soften or soften very slowly during cooking—for
usually assumed to follow the models described example, Chinese water chestnut, sugar beet, and
by Carpita and Gibeaut (1993). The cell wall itself beetroot. In Chinese water chestnut, the thermal
is an important constituent of produce, providing stability of texture is associated with the presence
dietary fiber, thought to protect against colorectal of ferulic acid in the cell wall (Waldron et al.
cancer (Harris et al. 1993). 1997).
Changes that occur in the cell wall during Postharvest treatments involving dipping or
ripening of fruit, storage of produce, and cooking infiltrating with calcium maintain firmness during
are critical to the texture of the final product. storage of a wide range of fruit (Conway et al.
During maturation of some vegetative parts, 1994). Examination of fracture surfaces following
especially stems and petioles, cell walls become tensile testing of apple cortex indicated that tissue
lignified (Okimoto 1948, Price and Floros failure from calcium-treated fruit was due to cell
1993). Lignification results in toughening of rupture, whereas failure in control apples was due
the product, such as woodiness in asparagus, to cell debonding (Glenn and Poovaiah 1990).
broccoli, pineapple, and rutabaga. During fruit While evidence suggests that calcium influences
ripening, cell wall changes include solublization texture through its interaction with the cell
and degradation of pectin and a net loss of wall (pectin), it may also affect texture through
the noncellulosic neutral sugars galactose and interactions with membranes.
arabinose, and there may be a decrease in the
molecular weight distribution of hemicelluloses The cell wall may also influence perception
(Harker et al. 1997). Numerous enzymes have of juiciness through its ability to hold and
been suggested as being critical to these changes release fluid. In some fruits, the cell wall swells
in the cell wall including polygalacturonases and considerably during ripening (Redgwell et al.
several glycosidases, including β-galactosidase, 1997). It has been suggested that hydrated cell
xyloglucanase, endotransglycosylase, and walls and perhaps the presence of free juice
cellulases (Dey and del Campillo 1984, Huber over the surface of undamaged cells could be
1992, Seymour and Gross 1996, Harker et al. responsible for the sensation of juiciness in fruit
1997). The possible role of expansins—proteins with soft melting textures (Harker et al. 1997).
that are proposed to disrupt hydrogen bonds In stonefruit, loss of juiciness is thought to occur
within the cell wall—has been considered (Civello when pectates bind water into a gel-like structure
et al. 1999). The use of molecular approaches, within the wall (Ben-Arie and Lavee 1971).
including antisense technologies, has been a Separation of cells at the middle lamella rather
powerful tool in the search for an understanding of than rupture of cells during chewing is at least
fruit softening (Giovannoni et al. 1989). However, partially responsible for the dry, mealy mouth-
no single enzyme has been identified as the major feel of overripe apples and wooliness of peaches
determinant of fruit softening, suggesting that wall (Harker and Hallett 1992).
breakdown results from the coordinated action of
several enzymes or that the key enzyme has not Cell Turgor
been identified.
Plant cells tend to maintain a small positive
pressure, known as turgor pressure, which
develops when the concentration of solutes inside
92
the cell (more specifically inside the plasma (Shackel et al. 1991). Also, turgor is thought to
membrane) is higher than that outside the cell. play a central role in softening and development
The extracellular solution fills the pores of the cell of mealiness during storage of apples (Hatfield
wall, sometimes infiltrates into gas-filled spaces, and Knee 1988).
and usually is continuous with vascular (water-
conducting) pathways of the plant. Differences in Cell-to-Cell Debonding Versus Cell Rupture
solute concentration at the inner and outer surface
of the plasma membrane cause water to flow into The strength of the cell wall relative to the
the cell by osmosis. This net movement of water adhesion between neighboring cells will determine
is halted by the physical constraint of the rigid cell whether cell rupture or cell-to-cell debonding is
wall and, as a result of this, turgor develops inside the mechanism of tissue failure. Cell rupture is
the cell. At equilibrium, generally associated with crisp and often juicy
Ψ = Ψp + Ψπ produce, as well as with unripe fruit and raw
Where: vegetables. Cell-to-cell debonding is frequently
Ψ is the turgor (generally a positive value), associated with dry, unpleasant texture such as
Ψp is the water potential (water activity, in mealy apples or chilling-injured stonefruit and
generally a negative value) of the tissue, and tomato and with juice loss in citrus (Harker et al.
Ψπ is osmotic pressure (generally a positive 1997).
value) of the cell (Tomos 1988).
However, a dry texture is not always unacceptable
Turgor has the effect of stressing the cell wall. The to consumers—in banana, for example. In some
consequences of this stressing depend on whether fruits, cell-to-cell debonding does not result in
compressive loads or tensile loads are applied. a dry texture; rather, a layer of juice covers the
When tissues are subjected to compressive loads, intact cells exposed following cell separation
higher-turgor tends to make the cell more brittle; (Harker et al. 1997). Furthermore, cell-to-cell
that is, makes it fail at a lower force (Lin and debonding is a common outcome of cooking
Pitt 1986). When tissues are subjected to tensile of vegetables such as potato (Waldron et al.
measurements, turgor tends to harden the cell wall 1997) and carrot (Ng and Waldron 1997). In
and a greater force is needed before cells fail (De fresh produce, cell adhesion is presumed to be a
Belie et al. 2000a). However, turgor is unlikely function of three factors: strength of the middle
to influence tissue strength if the mechanism lamella, the area of cell-to-cell contact, and the
of failure is cell-to-cell debonding, rather than extent of plasmodesmatal connections (Harker
fracturing across individual cells, unless an et al. 1997). Tissue collapse can also occur
increasing turgor, and thus swelling, reduces cell- without cell wall breakdown or cell separation.
to-cell contact area (Glenn and Poovaiah 1990, In some tissues, fluids are forced out of cells by
Harker and Hallett 1992). compressive forces known as “cell relaxation”
(Peleg et al. 1976) or “exosmosis” (Jackman and
The importance of turgor has been demonstrated Stanley 1995).
in a number of ways. The rapid phase of cooking-
induced softening of carrot occurs as a result of Other Elements of Tissue Strength
membrane disruption and the elimination of the
turgor component of texture (Greve et al. 1994). The strength and integrity of many edible plant
Similarly, when produce experiences a freeze- organs are influenced by a number of additional
thaw cycle, the membranes are damaged and the factors (Harker et al. 1997). Many fruits and
tissues become more flaccid in the case of leafy vegetables contain a number of tissue zones:
vegetables (and softer in the case of fruits) and periderm, pericycle, and phloem parenchyma in
often leak much juice upon thawing. Firmness carrot; skin, outer pericarp, inner pericarp, and
and turgor correlate well in apple (Tong et al. core in kiwifruit; and outer pericarp, locular gel,
1999), and turgor declines during tomato ripening seeds, and columella in tomato. These tissues
93
differ in strength and biological properties and proposed a texture profile, a systematic approach
often need to be considered individually when to sensory texture analysis based on mechanical,
measuring texture. For example, failure of the geometrical, and other characteristics. Mechanical
core of kiwifruit to soften to the same extent as characteristics included basic parameters
the pericarp causes a texture that is unacceptable (hardness, cohesiveness, viscosity, elasticity,
to consumers. In some multiple fruit that do and adhesiveness) and secondary parameters
not adhere to the receptacle, such as raspberry, (brittleness or fracturability, chewiness, and
the main element of strength is the adhesion gumminess). Geometrical properties related to
between neighboring drupelets due to hair-like size, shape, and orientation of particles. The
protuberances. However, it is the skin of many other characteristics comprised moisture and fat
types of produce that plays a key role in holding content. Sherman (1969) and others have proposed
the flesh together, particularly in soft fruit. The revisions of the texture profile classification
cuticle of epidermal cells and thickened cell walls scheme, but the original is generally used with
of hypodermal cells contribute to strength of only minor changes by sensory texture specialists.
simple skins. In harder, inedible skins, specialized Most sensory analysis textbooks contain a small
cells may be present: collenchyma, sclerenchyma, chapter on evaluation of texture; for example,
tannin-impregnated cells, and cork. Meilgaard et al. (1999). Harker et al. (1997)
reviewed fruit texture and included extensive
The presence of tough strands of vascular tissue discussion of oral sensation of textural attributes.
may strengthen the flesh but often results in
an unpleasant fibrous texture. For example, Shewfelt (1999) suggested that the combination
toughness of asparagus spears is principally due of characteristics of the product be termed
to fiber content and fiber lignification (Lipton “quality” and that the consumer’s perception and
1990). Rarely is the stringiness desirable, as in response to those characteristics be referred to as
spaghetti squash. In most commercial fruits, “acceptability.” Texture may be a limiting factor
with the exception of pineapple (Okimoto 1948), in acceptability if textural attributes are outside
fibrousness of the flesh is not a major problem. the individual’s range of acceptability for that
However, some fruits, including peaches and commodity. People have different expectations
muskmelons, can have a problem with stringiness and impose different limits for various
(Diehl and Hamann 1979). Generally, the commodities. The relationship of instrumental
perception of stringiness is greater with very measurements to specific sensory attributes and
ripe fruit due to the contrast between the soft their relationship to consumer acceptability must
melting texture of the parenchyma cells and the be considered (Shewfelt 1999). Instruments may
fibrousness of the vascular tissues. Similarly, be designed to imitate human testing methods,
the gritty texture of pear and guava (Harker et or fundamental mechanical measurements may
al. 1997) becomes particularly noticeable when be statistically related to human perceptions and
the surrounding cells are soft. However, while judgments to predict quality categories. Only
stringiness is caused by vascular tissues, grittiness people can judge quality, but instruments that
is caused by sclerenchymatous stone cells (Harker measure quality-related attributes are vital for
et al. 1997). research and inspection (Abbott et al. 1997).
People sense texture in numerous ways: the look The ability to measure texture is critical for
of the product, the feel in the hand, the way it evaluation and control of quality. The complex
feels as they cut it, the sounds as they bite and nature of texture is associated with the diversity
chew it, and, most important of all, the feel of of tissues involved, the attributes required to
the product in the mouth. Szczesniak (1963) describe textural properties, and changes in these
94
attributes as the product ripens and senesces. subsequent behavior of the material. On the other
Instrumental measurements are preferred over hand, scientists who study food are interested in
sensory evaluations for research and commercial initial failure but they are also interested in the
applications because they are less subject to continuous breakdown of the food in the mouth
human influences, are more precise, and can in preparation for swallowing. As Bourne (1982)
provide a common language among researchers, pointed out, “Food texture measurement might
companies, regulatory agencies, and customers. be considered more as a study of the weakness
It is often suggested that the relevance of of materials rather than strength of materials.” In
instrumental measurements depends on how well fact, both strength and breakdown characteristics
they predict sensory attributes (Voisey 1971), but are important components of texture.
there are also valid uses for mechanical property
measurements that relate only to functional
behavior of the fruit or vegetable, such as bruise Elastic and Viscoelastic Behavior
resistance or the ability to be sliced for fresh-cut
preparations. Fruits and vegetables exhibit viscoelastic behavior
under mechanical loading, which means that
There have been numerous reviews of methods for force, distance, and time—in the form of rate,
instrumental measurement of fruit and vegetable extent, and duration of load—determine the
texture (Bourne 1980, Chen and Sun 1991, value of measurements. For example, impact
Abbott et al. 1997, Harker et al. 1997). Interaction of the fruit against a hard surface is very rapid
among characteristics and the continuing loading, whereas the weight of other fruit on an
physiological changes over time complicate the individual fruit at the bottom of a bin and the
measurement of fruit or vegetable texture. For force of a carton wall against tightly packed fruit
example, as the parenchymal tissue of honeydew are long-term loads. The fruit will respond quite
melon softens, the perception of fibers (vascular differently to the two forms of loading. Because
bundles) increases (Diehl and Hamann 1979). On of the viscoelastic character of fruit and vegetable
the other hand, the fibrousness in asparagus is tissues, every effort should be made to use a
related to active lignification of fiber and vascular consistent action and speed when making manual
bundles (Chang 1987). Similar effects can affect texture measurements such as the Magness-Taylor
instrumental measurements. For example, fibers puncture test (Blanpied et al. 1978, Harker et al.
are held relatively rigidly in a hard melon and 1996). The rate of loading should be controlled
thus contribute to the overall force required to cut and specified in mechanized measurements.
through the flesh, but the fibers in a soft melon can The optimal rate of loading differs for different
be displaced by the instrument’s probe and alter commodities. People use different loading rates
distribution of forces within tissue. The displaced (chewing speeds) when eating foods of different
fibers can also effectively change the shape textures (Harker et al. 1997), but the optimum
of the probe as it progresses through the flesh loading rate for instrumental measurements
accumulating a “cap” of fibers. may not resemble the rate of human mastication
(Thybo et al. 2000).
Most instrumental measurements of texture have
been developed empirically. While they may There are many types of mechanical loading:
provide satisfactory assessments of the quality puncture, compression, shearing, twisting,
of produce, they often do not fulfill engineering extrusion, crushing, tension, bending, vibration,
requirements for fundamental measurements and impact. And there are four basic values that
(Bourne 1982). Fundamental material properties can be obtained from mechanical properties tests:
measurements were developed to study the force (load), deformation (distance, displacement,
strength of materials for construction or penetration), slope (ratio of force to deformation),
manufacture. After the failure point of such a and area under the force/deformation curve
material is exceeded, there is little interest in the (energy). The engineering terms based on these
95
measurements are stress, strain, modulus, and
energy, respectively.
96
Table 2. Magness-Taylor fruit firmness tester and related penetrometers
____________________________________________________________________________________________________________________
Instrument Type* Force† Readout Manufacturer
____________________________________________________________________________________________________________________
Magness-Taylor MT Manual Gauge D. Ballauff Manufacturing Co., Laurel, MD
Universal testing Universal Mechanical Electronic AMETEK Test and Calibration Instruments
machines or (Paoli, PA)
materials testers Chatillon (Largo, FL)
Food Technology Corp. (of General Kinetics,
Inc.)
Instron Corp. (Canton, MA)
Lloyd Instruments Material Testing Products
(Hampshire, UK)
Stable Micro Systems, Ltd. (Surrey, UK)
Tinius Olsen Testing Machine Co. (Willow
Grove, PA)
_____________________________________________________________________________________________________________________
*MT = Magness-Taylor tester.
†
MT firmness, regardless of instrument, is measred as force and should be reported in pounds-force (lbf, English units) or Newtons (N, metric units),
not in kilograms force (kgf), though the gauges of some instruments indicate kg. To convert: lbf x 4.448 = N; kgf x 9.807 = N; N x 0.225 = lbf; N x
0.102 = kgf.
‡
Other labels, such as “McCormick,” may occur on Effe-gi instruments.
97
A typical force/deformation (F/D) curve for a At the maximum deformation point specified by
cylindrical piece of apple tissue compressed at the user, the probe is withdrawn and the force
constant speed is shown in figure 1. F/D curves diminishes until contact is lost. In the apple tissue
for puncture tests look similar to compression shown in figure 1, maximum force occurred at
curves. The portion of the initial slope up to point maximum deformation, point (c), but other apples
(a) in figure 1 represents nondestructive elastic in the same lot had maxima at rupture point (a)
deformation; point (a) is the inflection point or at some point between rupture and maximum
at which the curve begins to have a concave- deformation, such as marked by point (b). Of
downward shape and is called the “elastic limit.” course, F/D curves that differ from the one shown
The region before point (a) is where slope or in figure 1 are also reported for apple and for other
elastic modulus should be measured. Beyond the commodities. F/D curves for very soft, noncrisp,
elastic limit, permanent tissue damage begins. or spongy tissues do not have sharp peaks but
There may be a bioyield point (b), at which cells show gradual increase in force to a rupture point,
start to rupture or move with respect to their followed by gradual decrease. Some may not
neighbors, causing a noticeable decrease in slope. even show rupture; for example, a cylinder of
Point (c) marks rupture, where major tissue failure eggplant compressed like apple tissue in figure
causes the force to decrease substantially. In some 1 may show smoothly increasing force to the
F/D curves, including figure 1, bioyield may not point of maximum deformation. Samples with a
be distinguishable from rupture. Beyond rupture, mixture of parenchyma and fibers or stone cells
the force may again increase, level off, or decrease may have quite jagged F/D curves, with several
as deformation increases (Bourne 1965). local maxima and ruptures as the probe encounters
resistant clusters of stone or fiber cells.
Figure 1. Actual force/deformation curve of a cylindrical piece of apple tissue under compression
of 1 mm s-1 demonstrating elastic limit (a), bioyield (b), and rupture or massive tissue failure (c).
Maximum force is at point (c) but could also occur at point (a) or point (b) in other apples. Force/
deformation (F/D) for Magness-Taylor puncture would look similar (with somewhat different
maximum forces) but would terminate at 5/16 in or 8 mm, depending on whether original or metric
specification was selected to control the universal testing instrument.
98
Firmness of horticultural products can be or mechanical use, and dial (analog) or digital
measured at different force or deformation levels readout (table 2). The term “Magness-Taylor
in all three regions of figure 1, depending on the firmness” is used generically for the measurements
purpose of the measurement and the definitions of made with the several variants of the MT. All
the quality attributes. F/D characteristics beyond use rounded-tip probes of specific geometry and
the elastic limit may be more important than those measure the maximum force required to insert
before it, because they simulate the destruction the probe 7.94 mm (5/16 in) into the flesh (Haller
that occurs in bruising or eating (Szczesniak 1941). The rounded portion of a Magness-Taylor
1963, Bourne 1968). The two most common probe is only a portion of a full hemisphere . A
texture tests of fruits and vegetables, the Magness- probe measuring 11.11 mm (28/64 in) in diameter
Taylor puncture and the Kramer Shear report with a radius of curvature of 8.73 mm (11/32 in) is
only the maximum force attained, regardless of used for apples. A probe measuring 7.94 mm (5/16
the deformation at which it occurs. On the other in) in diameter with a radius of curvature of 5.16
hand, elastic modulus or Young’s modulus is often mm (13/64 in) is used for cucumber, kiwifruit,
used by engineers as an index of product firmness. mango, papaya, peaches, pears, and plums. A thin
The modulus of elasticity is the ratio of stress slice of skin (about 2 mm thick and slightly larger
to strain as calculated from the slope of the F/D diameter than the probe) should be removed from
curve before the elastic limit. Any nondestructive the area to be tested, except for cucumbers, which
method should limit the force or deformation are tested with the skin intact.
level to the elastic region so that negligible
tissue damage is sustained during measurement. A group of U.S. researchers published
It is important to recognize and understand recommendations for making manual
the fundamental properties measured by both penetrometer tests (Blanpied et al. 1978), stating
destructive tests and nondestructive methods, the that steady force should be applied so that the
differences between them, and the factors that can probe is inserted to the inscribed depth mark in
affect the tests. 2 s. The probes can also be mounted in materials
testers (universal F/D testing machines) made by
Numerous mechanical instruments have been numerous manufacturers (Bourne 1974, Breene
developed over the past century for measuring et al. 1974, Abbott et al. 1976, Harker et al. 1996,
textural attributes of horticultural products. Lehman-Salada 1996). A group sponsored by
Despite the large variations in design, these the Commission of the European Communities
mechanical instruments either measure or control recommended that a materials tester be used to
functions of force, deformation, and time. The drive the probe to a depth of 8 mm at speeds
types of loading by these instruments include between 50 and 250 mm min-1 (Smith 1985).
puncture, compression, shearing, twisting, Because of the curvature of the MT probes and
extrusion, crushing, tension, and bending. the fact that firmness as measured in puncture
tests is a combination of shear and compression in
Puncture Tests variable proportions, it is not possible to convert
measurements made with one size MT probe to
Puncture testers based on the original Magness- the other MT size, or to accurately convert to
Taylor pressure tester (MT), also called the or from values for probes of other geometries
USDA or Ballauff tester (Magness and Taylor (Bourne 1982). A random sample of 20 to 30 fruit
1925, Haller 1941) and more correctly called of similar size and temperature should be tested
the Magness-Taylor fruit firmness tester, are with punches on two opposite sides, depending
used to measure firmness of numerous fruits and on uniformity of the lot. Peaches are often more
vegetables to estimate harvest maturity or for variable around the circumference than other fruit
postharvest evaluation of firmness. There are so a larger number is recommended (Blanpied
several adaptations of the Magness-Taylor tester et al. 1978). Similar measurements are made
that differ in instrument size and shape, manual on cherry, grape, and strawberry using a 3-mm
99
probe and on olive using a 1.5-mm probe on spaces between pieces would be expected to
the U.C. tester (E.J. Mitcham 2000, personal affect significantly the F/D profile as the blades
communication). Numerous puncture tests with penetrate through the contents of the shear
flat-faced cylindrical or hemispherical probes and cell; therefore some standardization of loading
a few with conical probes have been conducted. practice is advisable. Adaptations with smaller
None have achieved the acceptance of the cells and fewer blades are available; for example,
Magness-Taylor fruit firmness test. Stable Micro Systems. As with the MT probe,
comparisons should not be made between results
Shear Tests from cells of different geometries.
100
al. (1990) found that whole tomato compression and watermelon) usually break or rupture and cells
was relatively insensitive to small differences in from ripened soft fruits (banana, nectarine, and
firmness due to chilling injury. Kader et al. (1978) kiwifruit) tend to separate at the middle lamellae.
compressed tomatoes between a pair of spherical
indenters as a measure of firmness. Torsion Test
If the viscous element is a significant contributor True torsion tests are rarely used on horticultural
to the texture, as it is for intact tomatoes and specimens because of the difficulties in shaping
citrus, measurement of continuing deformation and holding the tissue (Diehl and Hamann 1979,
under a constant force (creep) (Hamson 1952, Diehl et al. 1979).
El Assi et al. 1997) or decrease in force under
a fixed deformation (relaxation) (Sakurai and Twist Test
Nevins 1992, Errington et al. 1997, Kajuna et
al. 1998, Wu and Abbott 2002) provides textural Studman and Yuwana (1992) proposed a simple
information in addition to elastic properties. twist tester consisting of a sharp spindle with a
To minimize the effect of loading position on rectangular blade that is forced into the flesh; the
firmness measurement in tomato, Kattan (1957) torque (twisting force) required to cause crushing
designed a creep tester that applied force around or yielding of the tissue is measured. Though
the fruit’s circumference with a belt. The failure called a “twist test,” this is not to be confused
of creep or force-relaxation testers to be adopted with a torsion test: The properties tested are
commercially is due to the time required for likely a combination of shear and compression.
adequate relaxation, which can be up to 60 s. Harker et al. (1996) found the twist test to be
more precise than several testers using the MT
Tension Test puncture probe; however, Hopkirk et al. (1996)
suggest that puncture and twist tests may measure
Tensile tests measure the force required to stretch different mechanical properties, resulting in quite
or pull a sample apart. Failure can be through cell different firmness judgments. The twist test has
rupture, cell separation, or a combination of both. the advantage of being able to measure strength
Tensile measurement has not been as popular of tissue zones at specific depths from the surface
as puncture or compression testing because it without requiring the excision of tissue samples.
is not intuitively as closely related to crushing
or chewing as is puncture or compression and
because it requires gripping or otherwise holding Nondestructive Measurements for Online
the ends of the sample so that they can be pulled Sorting
apart without crushing the tissues where they are
held. Schoorl and Holt (1983) used clamps to hold Most F/D measurements are destructive, such as
apple tissue. Stow (1989) and Harker and Hallett the familiar Magness-Taylor fruit firmness test
(1992) used shaped samples held by special and the Kramer shear test; or they are too slow
claw-like hooks. Harker and Hallett (1994) used for online use, such as the Cornell firmness tester.
quick-set adhesive to glue the ends to instrument However, remember that eating is destructive!
fixtures. Researchers often examine the broken Rupture forces usually provide the best correlation
ends of tensile test samples to determine the mode with sensory texture evaluations of foods.
of fracture. Microscopic analyses of the broken Unfortunately, destructive tests cannot be used
ends (Lapsley et al. 1992, Harker and Sutherland to sort fruits and vegetables for subsequent
1993, Harker and Hallett 1994, Harker et al. 1997) sale, so a great deal of research has gone into
reveal that tissue from unripe fruit generally developing nondestructive methods to estimate
fractures due to individual cells breaking, whereas the mechanical properties and the textural quality
cells from ripe fruits that tend to be crisp (apple of fruits and vegetables (Chen and Sun 1991,
101
Abbott et al. 1997, Hung et al. 2001). None of force, coefficient of restitution, contact time, and
these nondestructive methods has attained wide the impact frequency spectrum. The coefficient
commercial acceptance to date. of restitution is the ratio of the velocities of the
product just before and after impact and reflects
During development, new instrumental texture the energy absorbed in the product during impact.
measurements are most often initially calibrated
against existing instruments. If they are to be There is no agreement on the best parameter
used to predict sensory attributes or acceptability, to measure; selection seems to depend on
the new measurement should also be compared commodity, impact method, and the firmness
directly with descriptive sensory analyses to reference used by the investigators. Most impact
develop calibration equations for quantitative tests involve dropping the product onto a sensor
attributes (how much of a trait is present) or with (Rohrbach 1981, Delwiche et al. 1987, Zapp et
consumer evaluations to predict acceptability. al. 1990, McGlone and Schaare 1993, Patel et
Alternatively, instrumental measurements may al. 1993) or striking the product with the sensor
be compared with commercially useful traits (Delwiche et al. 1989, Brusewitz et al. 1991, Chen
like bruising, days from bloom, or storage et al. 1996, Bajema and Hyde 1998). Delwiche et
life to develop predictive equations. After the al. (1989, 1991) developed a single-lane firmness
relationship between an instrumental measurement sorting system for pear and peach. Impact
and a quality attribute or acceptability has been measurements often do not correlate highly
well established, the instrumental measurement with the Magness-Taylor puncture measurement
is usually used to replace human evaluations. It is (Hopkirk et al. 1996). A potential problem with
advisable to verify the relationships occasionally, impact tests is that bruising may occur unless a
because changes in factors such as genetics, soft sensor is developed (Thai 1994).
growing or storage conditions, consumer
preference, or wear on the instrument may change Sonic or Acoustic Tests. Sonic (acoustic)
the relationships. vibrations are those within the human audibility
range of 20 to about 20,000 Hz (vibrations per
Laser Air-Puff Test. A nondestructive, noncontact second). Sonic measurements provide a means
firmness detector was recently patented (Prussia of measuring fruit and vegetable firmness. The
et al. 1994) that uses a laser to measure deflection traditional watermelon ripeness test is based on
caused by a short puff of high-pressure air, the acoustic principle: One thumps the melon and
similar to some devices used by ophthalmologists listens to the pitch of the response.
to detect glaucoma. This is essentially a
nondestructive compression test. Under fixed air A number of sonic instruments and laboratory
pressure, firmer products deflect less than softer prototype sorting machines have been developed
ones. Laser-puff readings correlate well with and tested (Abbott et al. 1968, 1992, Armstrong
destructive Magness-Taylor firmness values for et al. 1990, Peleg et al. 1990, Zhang et al. 1994,
apple, cantaloupe, kiwifruit, nectarine, orange, Stone et al. 1998, Schotte et al. 1999, De Belie
pear, peach, plum, and strawberry (Fan et al. 1994, et al. 2000b, Muramatsu et al. 2000). When an
Hung et al. 1998, McGlone et al. 1999, McGlone object is caused to vibrate, amplitude varies
and Jordan 2000). with frequency of the vibration and will be
at a maximum at some particular frequency
Impact or Bounce Test. When one object collides determined by a combination of the shape, size,
with another object, its response is related to its and density of the object; such a condition is
mechanical properties, its mass, and the contact referred to as resonance. Resonance measurement
geometry. Numerous studies have been conducted can be achieved by applying an impulse or thump
on the impact responses of horticultural products that contains a range of frequencies. Modulus of
and a number of impact parameters have been elasticity values obtained from resonant frequency
proposed to measure firmness, including peak data have correlated well with those measured by
102
conventional compression tests, but they often a laser beam may change during ripening due to
correlated poorly with MT puncture forces. Abbott compositional changes and changes in cell-to-
et al. (1968) proposed a stiffness coefficient, f2m, cell contact. Measurement of the scatter using
which was based on the modulus of elasticity computer vision may thus provide an indirect
using the resonant frequency (f) and mass (m) of indication of textural changes. Significant
the specimen; this was later modified by Cooke correlations between mechanical properties and
and Rand (1973) to f2m2/3. image size have been shown in apples (Duprat et
al. 1995, McGlone et al. 1997, Cho and Han 1999,
Farabee and Stone (1991) developed a portable De Belie et al. 2000a) and tomatoes (Tu et al.
sonic instrument for field determination of 2000).
watermelon ripeness and hollow heart detection.
Kawano et al. (1994) reported a commercial Juiciness
sorting machine for detecting internal voids in
Japanese watermelon. Shmulevich et al. (1995) The importance of juiciness has been
developed a sonic instrument using a lightweight demonstrated by numerous consumer awareness
flexible piezoelectric film sensor to follow changes studies; however, there has been little progress
in fruit during storage. Muramatsu et al. (2000) in developing instrumental measurements of
examined the relationship of both phase shifts juiciness. Intuitively, one would expect total
and resonant frequencies to firmness. Nybom moisture content to determine juiciness, but the
(1962) and Peleg et al. (1990, 1999) examined correlations between them are often low for fruits
the sonic energy transmitted by the specimen and vegetables (Szczesniak and Ilker 1988).
rather than the resonant frequencies. Despite Apparently, inability of cells to release juice
considerable research, sonic vibration has not has a greater effect. For example, water content
yet become a viable option for the horticultural of juicy and chilling-injured peaches is similar,
industry. However, several advanced commercial yet injured fruit have a dry mouth-feel. Also,
prototypes are currently being evaluated. mealy apples feel dry to the palate because cells
separate at the middle lamella, rather than being
Ultrasonic Tests. Ultrasonic frequencies
ruptured and releasing juice during chewing.
(>20,000 Hz) are widely used in the medical field
Generally, juiciness is characterized as weight or
and for analyzing meat. Ultrasonic tests have
percentage of juice released from a fixed weight
been used with limited success for measuring
of tissue. Juice can be extracted from tissue by
physical and chemical properties of fruits and
the following methods: using a press (like a
vegetables because of the high attenuation
(energy absorption) of plant tissues. The cider press), homogenizing and centrifuging to
commonly measured ultrasonic parameters are separate juice from solids, using juice extractors,
velocity, attenuation, and frequency spectrum and measuring juice released during compression
composition. Bruises in apples (Upchurch et al. testing of excised tissue (Harker et al. 1997).
1987) and hollow heart of potatoes (Cheng and
Haugh 1994) could be detected in the laboratory Summary
using ultrasonics. Mizrach and Flitsanov (1999)
and Mizrach et al. (1994, 1999) have followed Texture measurement has become widely
the softening process in avocados, melons, and accepted by horticultural industries as a critical
mangoes, respectively. indicator of nonvisual aspects of quality. The
ability to measure texture has allowed industries
Light Scatter Imaging. As light passes through to set standards for quality at pack-out and to
tissue, cellular contents such as starch granules, monitor deterioration in quality during storage
cell walls, and intercellular spaces cause scatter. and distribution. Furthermore, the study of the
The extent of scatter of collumated light such as chemical, physiological, and molecular changes
that control or influence texture has been
103
facilitated by the development of methods for References
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Postharvest Pathology latent infections before harvest or occur at harvest
or afterward during storage. Fungi are more
Peter L. Sholberg and William S. Conway commonly found attacking fruit, and bacteria
are more common as postharvest pathogens of
Sholberg is with the Pacific Agri-Food Research vegetables. This chapter will provide a general
Centre, Agriculture and Agri-Food Canada, overview of the subject touching on noteworthy
Summerland, BC, Canada. Conway is formerly research where it can be used to illustrate
with the Food Quality Laboratory, Henry A. postharvest pathology. The reader is encouraged to
Wallace Beltsville Agricultural Research Center, consult the references for specific information on
Agricultural Research Service, U.S. Department the topics that are covered.
of Agriculture, Beltsville, MD. He is now retired.
Factors that Influence Postharvest
Introduction Pathology
Losses caused by postharvest diseases are greater Postharvest losses vary each year. Prevailing
than generally realized because the value of fresh weather while the crop is growing and at harvest
fruits and vegetables increases several-fold while contribute greatly to the possibility of decay.
passing from the field to the consumer (Eckert and Certain cultivars are more prone than others to
Sommer 1967). Postharvest losses are estimated to decay caused by specific pathogens. Resistance
range from 10 to 30% per year despite the use of of major apple cultivars to the fungi that cause
modern storage facilities and techniques (Harvey blue mold, gray mold, bull’s-eye rot, and Mucor
1978). rot depended on cultivar (Spotts et al. 1999).
Condition of the crop, as determined by fertilizer
Postharvest diseases affect a wide variety of crops, and soil factors, is very important in susceptibility
particularly in developing countries that lack of the crop to disease. Maturity of the crop at
sophisticated postharvest storage facilities (Jeffries harvest, handling, and type of storage have great
and Jeger 1990). Infection by fungi and bacteria influence on how long the crop can be stored
may occur during the growing season; at harvest without decay. The following sections address
time; during handling, storage, transport and how these preharvest factors lead to disease in
marketing; or even after purchase by the consumer specific crops.
(Dennis 1983). Reduction of losses in perishable
food crops resulting from postharvest diseases Weather. Weather affects many factors related
has become a major objective of international to plant diseases, from the amount of inoculum
organizations (Kelman 1989). The reality is that that overwinters successfully to the amount of
there is a portending food crisis that will require pesticide residue that remains on the crop at
the concerted efforts of all who are involved harvest (Conway 1984). Abundant inoculum
in food production to redouble their efforts. In and favorable conditions for infection during the
fact, to adequately feed the world’s expected 10 season often result in heavy infection by the time
billion people within the next 40 to 50 years, food the produce is harvested. For example, conidia
production efficiency and distribution will need to of the fungus that causes bull’s-eye rot are rain-
be improved immensely (Campbell 1998). dispersed from cankers and infected bark to fruit,
especially if rainfall is prolonged near harvest
Specific causes of postharvest losses of fruits time, resulting in rotten fruit in cold storage
and vegetables may be classed as parasitic, several months later (Spotts 1990).
nonparasitic, or physical (Cappellini and Ceponis
1984).This chapter deals with the parasitic causes Pinpoint or storage scab of apple caused by the
that are of microbiological origin that begin as same fungus that causes apple scab and gray mold
caused by the fungus Botrytis cinerea are also
111
very much influenced by the weather. Storage and can be stored longer before they breakdown
scab only occurs in years with unusually wet or decay. Conversely, high nitrogen content in
summers and early falls, when the fruit remain wet fruit predisposes them to decay (Conway 1984).
for a day or more. These late-season infections In pears, it has been found that management
may not become visible until the apples are in of trees for low nitrogen and high calcium in
storage (Pierson et al. 1971). Flowers and fruit the fruit reduced severity of postharvest fungal
are most vulnerable to Botrytis cinerea infection decay (Sugar et al. 1992). Apple cultivars can
when conditions are wet. For example, in grapes be selected for resistance to certain postharvest
infection occurs at 15 to 20 ºC (59 to 68 ºF) in diseases (Spotts et al. 1999). For example, ‘Royal
the presence of free water after approximately Gala’ is extremely resistant to wound pathogens,
15 h (Bulit and Dubos 1988). In wet seasons, ‘Granny Smith’ to skin punctures, and ‘Braeburn’
strawberries and raspberries may be harvested in to infiltration of fungal spores into the core.
apparently sound condition only to decay during
transit and marketing (Snowdon 1990). Fungicide Sprays. Certain preharvest sprays
are known to reduce decay in storage. Several
Postharvest decay involves further development of studies done on the effectiveness of preharvest
preharvest infections together with new infections ziram fungicide application on pome fruit
arising from germination of spores on the fruit showed an average reduction in decay of about
surface. From these examples it is apparent that 25 to 50% with a single spray (Sugar and Spotts
decay often has a weather component, making 1995). Iprodione was used for several years as a
thorough weather records an important source preharvest spray 1 day before harvest to prevent
of information for predicting possible decay in infection of stone fruit by Monilinia spp. In
storage. combination with wax and/or oil, its decay control
spectrum is increased and it will also control
Physiological Condition. Condition of produce postharvest fungi such as Rhizopus and Alternaria
at harvest determines how long the crop can be (Ogawa et al. 1992). Cyprodinil prevented gray
safely stored. For example, apples are picked mold infection in apple 3 months after it was
mature but preclimacteric to ensure that they can applied (Sholberg and Bedford 1999). The new
be stored safely for several months. The onset class of strobilurin fungicides promises to provide
of ripening and senescence in various fruit and postharvest control of several diseases in fruits
vegetables renders them more susceptible to and vegetables, is especially effective against fruit
infection by pathogens (Kader 1985). On the other scab on apples, and should reduce the presence of
hand, fruit and vegetables can be made less prone pinpoint scab in storage.
to decay by management of crop nutrition. For
example, calcium has been more closely related to Packing Sanitation. It is important to maintain
disease resistance than any other cation associated sanitary conditions in all areas where produce is
with the cell wall (Sams 1994). packed. Organic matter (culls, extraneous plant
parts, and soil) can act as substrates for decay-
In a study on the effect of increased flesh calcium causing pathogens. For example, in apple and
content of apples in storage, fruit were treated pear packinghouses, the flumes and dump tank
with solutions of CaCl2 by dipping, vacuum, or accumulate spores (Blanpied and Purnasiri 1968)
pressure infiltration. Both vacuum and pressure and may act as sources of contamination if steps
infiltration increased calcium content of the fruit are not taken to destroy or remove them.
sufficiently to significantly reduce decay (Conway
1982). Increased calcium contents in potatoes Chlorine readily kills microorganisms suspended
and peaches have also been documented with in dump tanks and flumes if the amount of
reduced postharvest decay (Conway 1989). In available chlorine is adequate. A level of 50 to
general, produce containing adequate levels of 100 ppm of active chlorine provides excellent
calcium do not develop physiological disorders
112
fungicidal activity (Spotts and Peters 1980). following conditions (Ogawa and Manji 1984):
Chlorine measured as hypochlorous acid can • Type of pathogen involved in the decay
be obtained by adding chlorine gas, sodium • Location of the pathogen in the produce
hypochlorite, or dry calcium hypochlorite. Though • Best time for application of the treatment
chlorine effectively kills spores in water, it does • Maturity of the host
not protect wounded tissue against subsequent • Environment during storage, transportation,
infection from spores lodged in wounds. Organic and marketing of produce
matter in the water inactivates chlorine, and levels
of chlorine must be constantly monitored. The use Chemical Control
of a sand filter in association with chlorination
improves its efficiency probably because it Several fungicides are currently used as
removes organic matter (Sholberg and Owen postharvest treatments for control of a wide
1990). Chlorine is sensitive to pH (Dychdala spectrum of decay-causing microorganisms.
1983): Hypochlorite solutions with higher pH However, when compared with preharvest pest-
values (7.5 to 8.5) are more stable but less control products, the number is very small. Many
fungicidal, whereas at lower pH values (5.5 to 6.5) products formerly used after harvest are no longer
the solutions are less stable but more fungicidal. permitted because of concerns with residues and
possible toxic effects, the most notable being
Chlorine dioxide can replace hypochlorite in products that contain benomyl. Other products are
some sanitizing processes, because several no longer as effective because of development of
disadvantages limit the use of chlorine, including resistance by the target pathogen. For example,
its unpleasant odor. Chlorine dioxide is not intensive and continuous use of fungicides for
corrosive and is effective over a wide pH range control of blue and green mold on citrus has led
(Spotts and Peters 1980). Recently in precisely to resistance by the causal pathogens of these
controlled tests in water or as a foam, chlorine diseases (Eckert 1988). Resistance has been
dioxide was found to be effective against common reported in many other crops to several different
postharvest decay fungi on fruit packinghouse fungicides with different modes of action (Delp
surfaces (Roberts and Reymond 1994). Peracetic 1988). Resistance development continues to
acid is another material that could be used (Mari be a major problem and has resulted in the
et al. 1999). It has greater stability and faster Fungicide Resistance Action Committee (FRAC),
biocidal properties than chlorine dioxide but is a cooperative effort among various producers of
more corrosive. fungicides to delay resistance by recommending
specific management guidelines (FRAC 1998).
The search goes on for effective and economical
sanitizing agents. New and old products alike are Examples of chemicals currently used for
continually being evaluated under present-day postharvest treatments are thiabendazole,
packing operations. Interest in ozone has been dichloran, and imazalil. However, resistance
rekindled with development of more efficient to thiabendazole and imazalil is widespread
ozone generators. Acetic acid in the form of a gas (Holmes and Eckert 1999), and their use as
can be used as a sanitizing agent on several crops effective materials is declining. Preservatives or
(Sholberg 1998). It was as effective as SO2 in antimicrobial food additives are not generally
preventing gray mold decay in table grapes stored thought of as postharvest treatments, but they do
for 2 months (Sholberg et al. 1996). control decay and, in some cases, are the only
means of control. These products include sodium
Postharvest Treatments. Products or treatments benzoate, the parabens, sorbic acid, propionic
used to control postharvest decay can be classified acid, SO2, acetic acid, nitrites and nitrates, and
as either chemical or biological and should be antibiotics such as nisin (Chichester and Tanner
selected only after proper consideration of the 1972). In California, for example, gray mold of
stored table grapes is prevented by fumigation
113
with SO2 (Luvisi et al. 1992). The demand for Other bacterial microorganisms have been
new postharvest fungicide treatments is strong, developed for postharvest disease control.
especially since the loss of iprodione in 1996. For example, strains of Bacillus pumilus and
Pseudomonas fluorescens have been identified that
Fludioxinil was granted an emergency registration exhibit successful control of B. cinerea in field
in 1998 to curb potential losses in nectarines, trials of strawberry (Swalding and Jeffries 1998).
peaches, and plums (Forster and Adaskaveg Yeasts such as Pichia guilliermondii (Wisniewski
1999). Not all postharvest pathogens are currently et al. 1991) and Cryptoccocus laurentii, which
controlled by materials that are available. For occur naturally on apple leaves, buds, and fruit
example, Mucor piriformis, a major postharvest (Roberts 1990), were the first to be applied for
pathogen of apples and winter pears in the Pacific control of postharvest decay on fruit. The yeast
Northwest is not controlled by any registered Candida oleophilia has been registered for control
fungicide (Spotts and Dobson 1989). There is a of postharvest decay on fruit crops. The yeasts
dire need for new fungicide treatments that could Cryptococcus infirmo-minutus and Candida sake
in part be alleviated by using biological control successfully control brown rot and blue mold on
agents (Wisniewski and Wilson 1992, Utkhede sweet cherry (Spotts et al. 1998) and on three
and Sholberg 1993). diseases of apple (Vinas et al. 1998), respectively.
114
cases, 3.0 kGy (300 krad) have been effective in generally is more effective, hot air has been used
controlling decay in several products (Hardenburg to control decay in crops that are injured by hot
et al. 1986). A dose of 250 grays (Gy) has an water. Heating of pears at temperatures from 21
adverse effect on grapefruits, increasing skin to 38 ºC (70 to 100 °F) for 1 to 7 days reduced
pitting, scald, and decay. Low doses of 150 Gy postharvest decay (Spotts and Chen 1987). Decay
for fruit flies and 250 Gy for codling moth are in ‘Golden Delicious’ apples was reduced by
acceptable quarantine procedures (Meheriuk exposure to 38 ºC (100 °F) for 4 days (Sams et al.
and Gaunce 1994). Commercial application 1993) and virtually eliminated when treated after
of gamma radiation is limited by the cost and inoculation (Fallik et al. 1995, Klien et al. 1997).
size of equipment needed for treatment and by Heat treatment eliminates incipient infections and
uncertainty about consumers’ acceptance of improves coverage by fungicides (Couey 1989).
irradiated foods (Hardenburg et al. 1986). Gamma The primary obstacle to the widespread use of
irradiation may be used more in the future once heat to control postharvest fruit diseases or insect
methyl bromide is no longer available to control infestation is the sensitivity of many fruit to the
insect infestation in stored products. All uses of temperatures required for effective treatment.
methyl bromide are being phased out to avoid any
further damage to the protective layer of ozone Both low and high RH have been related to
surrounding the earth. postharvest decay control. Perforated polyethylene
bags for fruit and vegetable storage create RH
Effect of Storage Environment on Postharvest about 5 to 10% above that in storage rooms.
Decay Though shrivel and weight loss are reduced,
decay may increase (Spotts 1984). Crops with
Commercial producers and handlers modify well-developed cuticle and epidermis, such as
temperature, RH, and atmospheric composition apples and pears, tolerate lower RH levels, which
during prestorage, storage, and transit to control helps prevent storage decay. Often fungal spore
decay (Spotts 1984). For optimum decay germination is inhibited at low RH, and small
control, two or more factors often are modified differences in RH can have significant effects in
simultaneously. relation to the degree of postharvest decay (Spotts
and Peters 1981).
Temperature and RH. Proper management of
temperature is so critical to postharvest disease Modified or Controlled Atmospheres.
control that all other treatments can be considered Alterations in O2 and CO2 concentrations are
as supplements to refrigeration (Sommer 1989). sometimes provided around fruit and vegetables
Fruit rot fungi generally grow optimally at 20 (Spotts 1984). With close control of these gases,
to 25 ºC (68 to 77 °F) and can be conveniently the synthetic atmosphere is commonly called
divided into those with a growth minimum of 5 a “controlled atmosphere”; the term “modified
to 10 ºC (41 to 50 °F) and those with a growth atmosphere” is used when there is little possibility
minimum of -6 to 0 ºC (21 to 32 °F). Fungi with of adjusting gas composition during storage
a minimum growth temperature below -2 ºC (28 or transportation (Sommer 1989). Because the
°F) cannot be completely stopped by refrigeration pathogen respires as does produce, lowering O2
without freezing the fruit. However, temperatures or raising CO2 above 5% can suppress pathogenic
as low as possible are desirable because they growth in the host. In crops such as stone fruits, a
significantly slow growth and thus reduce decay. direct suppression occurs when fungal respiration
and growth are reduced by the high CO2 of the
High temperature may be used to control modified atmosphere. For example, CO2 added to
postharvest decay on crops that are injured by air has been widely used in the transport of ‘Bing’
low temperatures, such as mango, papaya, pepper, cherries, primarily to suppress gray mold and
and tomato (Spotts 1984). Though hot water brown rot. Low O2 does not appreciably suppress
fungal growth until the concentration falls below
115
2%. Important growth reductions result if O2 is Postharvest Diseases of Vegetables
lowered to 1% or less, though there is a danger
that the crop will start respiring anaerobically and Postharvest diseases of vegetables are caused by
develop off flavors. Other technologies that have microscopic fungi and bacteria (Snowdon 1992,
been tested for lowering postharvest decay with Howard et al. 1994). Bacteria are more common
limited success are storage and transport under as pathogens of vegetables than fruit because,
low O2 and the use of carbon monoxide (Spotts in general, vegetables are less acidic than fruit.
1984, Sommer 1989). Bacteria are visible under the light microscope
as mostly single-celled rods. Bacteria are capable
of very rapid multiplication under the right
Postharvest Diseases of Fruits conditions of pH, temperature, and nutrition.
They are classified according to their size,
Fruit crops are attacked by a wide range of shape, reaction to certain stains, and behavior on
microorganisms in the postharvest phase various growth media (Krieg and Holt 1984). The
(Snowdon 1990, Ogawa and English 1991). Actual term “vegetable” encompasses a range of plant
disease only occurs when the attacking pathogen parts, and the common definition is a culinary
starts to actively grow in the host. Diseases are one, denoting consumption as a savory rather
loosely classified according to their signs and than as a dessert food (Snowdon 1992). Many
symptoms. Signs are visible growths of the causal vegetables are fruits in the botanical sense, notable
agents, and symptoms the discernible responses examples being tomatoes, peppers, squashes, and
produced by the host. In many diseases there is cucumbers. Table 2 lists many of the important
local discoloration and disruption of tissue, with diseases of vegetable crops according to host and
the formation of obvious lesions. Postharvest causal agents.
diseases are caused primarily by microscopic
bacteria and fungi, with fungi the most important
causal agent in fruit crops.
116
Table 1. Important postharvest diseases of fruit
______________________________________________________________________________________
Fruit Disease Causal agent Fungal class/type
______________________________________________________________________________________
117
Table 1. Important postharvest diseases of fruit—Continued
______________________________________________________________________________________
Fruit Disease Causal agent Fungal class/type
______________________________________________________________________________________
Mango Anthracnose C. gloeosporioides Coelomycete
Botryodiplodia rot B. theobromae Coelomycete
Stem-end rots B. theobromae, Coelomycete
Phomopsis spp.
118
Table 2. Important postharvest diseases of vegetables
__________________________________________________________________________________
Vegetable Disease Causal Agent Fungal Class/Type
__________________________________________________________________________________
Bulbs Bacterial soft rot Erwinia caratovora Bacterium
(onion, garlic) Black rot Aspergillus niger Hyphomycete
Blue mold rot Penicillium spp. Hyphomycete
Fusarium basal rot Fusarium oxysporum Hyphomycete
Neck rot Botrytis spp. Hyphomycete
Purple blotch Alternaria porri Hyphomycete
Sclerotium rot Sclerotium rolfsii Agonomycete
Smudge Colletotrichum circinans Coelomycete
119
Table 2. Important postharvest diseases of vegetables—Continued
__________________________________________________________________________________
Vegetable Disease Causal Agent Fungal Class/Type
__________________________________________________________________________________
Roots/tubers
Carrots Bacterial soft rot Erwinia spp., Bacteria
Pseudomonas spp.
Black rot A. radicina Hyphomycete
Cavity spot Disease complex Soil fungi
Chalaropsis rot Chalara spp. Hyphomycete
Crater rot R. carotae Agonomycete
Gray mold rot B. cinerea Hyphomycete
Sclerotium rot S. rolfsii Agonomycete
Watery soft rot Sclerotinia spp. Discomycete
120
Table 2. Important postharvest diseases of vegetables—Continued
__________________________________________________________________________________
Vegetable Disease Causal Agent Fungal Class/Type
__________________________________________________________________________________
Sclerotium rot S. rolfsii Agonomycete
Soil rot R. solani Agonomycete
Sour rot Geotrichum candidum Hyphomycete
Watery soft rot Sclerotinia spp. Discomycete
Miscellaneous
121
New Directions for Postharvest Plant plant pathogens and foodborne human pathogens
Pathology such as Salmonella and Listeria. A study involving
more than 400 samples each of healthy and soft-
Postharvest plant pathology has changed its rotted commodities collected in retail markets
emphasis in the last decade. Food safety has indicated that the presence of Salmonella on
emerged as a key element in decay control produce affected by bacterial soft rot was twice
programs. Continued failure to effectively control that of healthy samples (Wells and Butterfield
certain postharvest diseases and the need for more 1997). Controlled experiments with potato,
environmentally friendly crop control materials carrot, and pepper tissues inoculated with a strain
require a new approach to disease control. of Salmonella confirmed that bacterial soft rot
Integrated postharvest decay control is the concept infection increased multiplication of Salmonella
that offers the most promise for the future. Society by at least three- to ten-fold compared with
can no longer rely on one or two control strategies multiplication on uninfected tissues. Similarly,
but must enlist the entire spectrum of strategies to populations of Listeria monocytogenes, inoculated
reduce postharvest losses. into decayed apple tissue, continually increased
on fruit decayed by Glomerella cingulata but
did not survive after 5 days on fruit decayed by
Food Safety Issues Penicillium expansum (Conway et al. 2000). The
pH of the decayed area declined from pH 4.7 to
The two most important causes of unsafe food are 3.7 in the case of P. expansum but increased from
microbial toxins (Hsieh and Gruenwedel 1990) pH 4.7 to 7.0 in the case of G. cingulata. This
and contamination of horticultural products by pH modification may be responsible for affecting
fecal coliforms (Gould 1973). The microbial growth of the foodborne pathogen.
toxins can be subdivided into bacterial toxins
and toxins produced by fungi or mycotoxins. An Contamination of produce with human pathogens
example of a microbial toxin that is extremely is an important issue that must be addressed
toxic are the botulinum toxins produced by the along with limiting decay caused by postharvest
anaerobic bacterium Clostridium botulinum. pathogens and maintaining product quality.
122
• Maintain good sanitation in packing areas References
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127
Flavor Genetics is the primary determinant of flavor of
fresh produce (Cunningham et al. 1985, Baldwin
Elizabeth A. Baldwin et al. 1991b, 1992), with preharvest environment
(Romani et al. 1983), cultural practices (Wright
Baldwin was with the Citrus and Subtropical and Harris 1985), harvest maturity (Fellman et
Products Laboratory, Agricultural Research al. 1993, Maul et al. 1998, Baldwin et al. 1999a),
Service, U.S. Department of Agriculture, Winter and postharvest handling (Mattheis et al. 1995;
Haven, FL. She is now with the U.S. Horticultural Fellman et al. 1993; Baldwin et al. 1999a,b)
Research Laboratory, Agricultural Research having lesser effect. Fruit such as apples and
Service, U.S. Department of Agriculture, Fort bananas that continue to ripen after harvest are
Pierce, FL. termed climacteric, while those such as citrus
and strawberries that do not ripen after harvest
are termed nonclimacteric. The flavor quality
Introduction of nonclimacteric fruit generally declines after
harvest, while climacteric fruit can reach their
The quality of fresh produce has traditionally best flavor after harvest. Climacteric fruit develop
been based on external characteristics of size, better quality if harvested after the start of
color, and absence of surface defects. Fruit and ripening, while fruit of both will be inferior in
vegetable breeders select for color, size, disease quality if harvested immature, even if held under
resistance, yield, and other easily quantified optimal postharvest conditions.
horticultural traits. Because flavor and texture
characteristics were not a part of the selection Human perception of flavor is exceedingly
process, improvements in these quality attributes complex. Taste is the detection of nonvolatile
have not kept pace with the more easily quantified compounds (in concentration of parts per hundred)
traits. Public and industry organizations are by several types of receptors in the tongue for
increasingly concerned with the public’s growing sugars or polyalcohols, hydronium ions, sodium
dissatisfaction over the flavor and texture of some ions, glucosides and alkaloids, etc. These
horticultural produce. correspond to the perception of sweet, sour, salty
and bitter tastes in food. Aroma compounds can
Flavor and aroma are perhaps the most elusive be detected in parts-per-billion concentrations and
and subjective of quality traits. Flavor is taste are detected by olfactory nerve endings in the nose
plus odor and is mainly composed of sweetness, (DeRovira 1997). The brain processes information
sourness, and aroma, which correspond to from these senses to give an integrated flavor
sugars, acids, and volatiles. Other components experience. This integration makes it difficult to
of flavor include bitterness related, for example, determine the relative importance of each input
to sesquiterpene lactones in chicory (Peters and since the brain can interpret changes in aroma as
Amerongen 1998), saltiness due to various natural changes in taste (O’Mahony 1995) or vice versa.
salts, and astringency related to flavonoids, For example, the levels of aroma compounds
alkaloids (DeRovira 1997), tannins (Taylor influenced panelist perception of sweetness and
1993), and other factors. The perception of sourness for tomatoes (Lycopersicon esculentum
sweetness—that is, sugars—one of the most Mill.) (Baldwin et al. 1998). Conversely, levels of
important components of fruit or vegetable taste components influenced panelist perception of
flavor, is modified by sourness or acid levels and aromatic descriptors in mango (Mangifera indica
aroma compounds. The contribution of aroma L.) (Malundo et al. 2000a). The perception of
to the flavor quality of fresh produce has gained certain combinations of chemicals is synergistic,
increasing attention. while others combinations mute our perception
in a process called masking. In contrast to
128
masking is anesthetization or blanking, in which Taste Components
olfactory receptors become overloaded. Lighter-
aroma volatiles (for example, top notes—low Fructose, sucrose, and glucose are the sugars that
molecular weight, polar, hydrophilic compounds) affect the perception of sweetness in fruits and
are perceived first and generally have the major vegetables. Fructose is the sweetest, and glucose
impact on perception, while heavier compounds is less sweet than sucrose. A single “sucrose
are perceived later: for example, background equivalent” value is the weighted average of these
notes—high molecular weight, nonpolar, various sugars in a sample (Koehler and Kays
hydrophobic compounds (DeRovira 1997). 1991). Sugar content is commonly accepted to be
synonymous with SSC (soluble solids content),
and an inexpensive refractometer can easily
Sensory Evaluation measure SSC. However, the quantification of
individual sugars requires complicated laboratory
Human perception of flavor can be determined analysis. Breeders often select for higher SSC in
by sensory evaluation by taste panels. an attempt to increase sweetness. In some fruits,
Consumer preference and acceptance vary due such as orange (Citrus sinensis), SSC relates to
to socioeconomic, ethnic, and geographical sweetness; while in others, such as tomato and
background, often necessitating the segmenting of mango, the relationship is not linear (Baldwin et
subpopulations for a particular study (O’Mahony al. 1998, 1999a, Malundo et al. 2000a).
1995). Generally a large number of panelists (for
example, 50 to 100) rank their perceptions on a Organic acids, such as citrate in citrus and
traditional 9-point hedonic scale, but sometimes tomatoes, tartaric acid in grapes (Vitis sp.), and
a simple 3-point scale including the descriptive malic acid in apples (Malus pumila), give fruit
terms outstanding, acceptable, and unacceptable and vegetables their sour flavor. Some fruits, like
can be effective for tomato fruit evaluation. In melon (Cucumis melo) or banana (Musa sp.), have
one study, adaptation of logistic regression from very little acid (Wyllie et al. 1995). Different acids
medical science proved useful, in which a 0 can affect sourness perception, depending on their
or 1 indicates whether the consumer would or chemical structure. An increase in carboxyl groups
would not purchase a mango (Mangifera indica). decreased acidity, while an increase in molecular
The consumer was asked to base their decision weight or hydrophobicity increased sourness
on flavor, which was then related to chemical (Hartwig and McDaniel 1995). For example,
constituents (Malundo et al. 2000b). Difference acetic acid was perceived as more intensely sour
testing can be used to measure slight differences than lactic or citric acid.
between foods (usually due to one particular
aspect of flavor) and is considered a narrow- Acids can be measured individually by HPLC
band approach. Descriptive analysis measures (Baldwin et al. 1991a,b), by titration (TA) with
intensities of a set of sensory attributes and is sodium hydroxide (Jones and Scott 1984), or by
considered a broadband approach (O’Mahony pH (Baldwin et al. 1998). Sometimes SSC, the
1995). Panelists are trained to detect a range ratio of SSC/TA, or pH relate better to sourness
of flavor attributes and score their intensity, than TA itself (Baldwin et al. 1998, Malundo et al.
generally on a 150 mm unstructured line. Sensory 2000a).
studies for fresh produce can be used to identify
optimal harvest maturity, evaluate flavor quality
in breeding programs, determine optimal storage
and handling conditions, assess effects of
disinfestation or preconditioning techniques on
flavor quality, and measure flavor quality over the
postharvest life of a product.
129
Aroma Components determined concentrations, odor thresholds, and
log odor units for those tomato volatiles present
Volatiles that we can perceive contribute to food at levels of 1 ppb or more (about 30 of > 400
flavor. The level at which a compound can be identified compounds). However, the aroma
detected by smell (the odor threshold) can be perception of volatile compounds is affected by
established in a background similar to a food the medium of evaluation. For example, both
medium as described by the Ascending Method the thresholds and descriptors of some volatile
of Limits of the American Society for Testing compounds in tomato were different if the
and Materials (ASTM 1991). Log odor units are background medium contained levels of methanol
calculated from the ratio of the concentration and ethanol similar to that found in fresh tomato
of a component in a food to its odor threshold. homogenate or in deodorized homogenate itself
Compounds with positive odor units contribute than if the medium was water (Tandon et al. 2000)
to food flavor. Buttery (1993), for example, (table 1).
130
Aroma compounds are often only released port where the volatiles are desorbed. It has been
upon cell disruption when previously used on apples, tomatoes (Song et al. 1997), and
compartmentalized enzymes and substrates strawberries (Song et al. 1997, Golaszewski et al.
interact (Buttery 1993). Some aroma compounds 1998).
are bound to sugars as glycosides (celery
[Apium graveolens], lettuce [Lactuca sativa]), Aside from GC and GC-MS methods, there are
or glucosinolates (cabbage [Brassica oleracea], new sensors available that have a broad range of
radish [Raphanus sativus]). This linkage can be selectivity. These sensor arrays, called “electronic
cleaved by enzyme action or heat during cooking. noses,” are useful to discriminate among samples
Other aromatic compounds are breakdown based on the interaction of volatile components
products of lipids, amino acids, lignin, or pigments with the various sensors. The resulting
(Buttery and Ling 1993). response pattern allows a particular sample or
flavor component(s) to be detected by pattern
Measurement of aroma compounds is difficult recognition. However, these instruments do not
and time consuming. Earlier studies employed give information that leads to identification and
the classical flavor isolation procedures of steam quantification of individual compounds. Five basic
distillation and solvent extraction (Teranishi and sensor technologies have been commercialized
Kint 1993). The disadvantage of this method to date: metal oxide semiconductors, metal oxide
is that it can qualitatively and quantitatively semiconductor field effect transistors, conducting
modify the flavor profile of a sample (Schamp organic polymers, piezoelectric crystals (bulk
and Dirinck 1982). This method is not easily acoustic wave), and quartz crystal microbalance.
applied to large numbers of samples, and internal The next generation of electronic noses may use
standards must be incorporated to determine fiberoptic, electrochemical, and bimetal sensors
recovery. The resulting concentration of material, (Schaller et al. 1998).
however, allows identification of compounds by
gas chromatography-mass spectrometry (GC-
MS). Investigators have employed purge-and-trap Relating Sensory to Chemical Data
headspace sampling methods, which involve
trapping and concentrating volatile components Chemical analysis of flavor compounds provides
on a solid support. Volatiles are later released little insight into the actual flavor experience.
from the trap using heat for analysis by GC-MS. However, sensory attributes, preferences, and
This method is excellent for quantification and decisions can be statistically related to chemical
identification of aroma compounds (Schamp and components in foods (Martens et al. 1994). Some
Dirinck 1982, Teranishi and Kint 1993). important or abundant flavor compounds in
selected fruits and vegetables are shown in table
Static headspace methods are said to more 2. Correlation of physical measurements with
closely reflect the true flavor profile; but sensory analysis gives meaning to instrumental
compounds are present at low levels, and some data, as was shown with apple and tomato
may not be detected. Cryofocusing (cold trap) of (Baldwin et al. 1998). For example, linear
static headspace volatiles (Teranishsi and Kint regression established relationships between levels
1993) reduces this problem, since samples are of sesquiterpene lactones and bitterness in chicory
concentrated without heating that may cause (Peters and Amerongen 1998). Multivariate
adulteration. This method has been used for methods require large data sets, but nonlinear
quantification of orange juice volatiles (Moshonas regression techniques such as principal component
and Shaw 1997). The newest method is solid- or discriminate analysis yielded useful results for
phase micro-extraction (SPME), a rapid sampling citrus (Moshonas and Shaw 1997), strawberry
technique in which volatiles interact with a fiber- (Fragaria ananassa) (Shamaila et al. 1992), and
coated probe inserted into the sample headspace. tomato (Maul et al. 1998). Differences between
The probe is then transferred to a GC injection
131
samples were found based on measurement of levels of flavor volatiles varied significantly
volatiles or other flavor compounds. among varieties (Baldwin et al. 1991a,b).
Insertion of the rin gene to reduce ethylene
Alternatively, sniff ports (olfactometry detectors) production and slow tomato fruit softening
can be used with GCs, allowing a person to resulted in some deterioration in flavor quality
determine if odors are detectable as well as their (Baldwin et al. 2000) and reduction in flavor
relative intensity as the volatile components are volatiles (Baldwin et al. 1992, 2000). Flavor
separated by the GC column. This technique was appears to be related to ethylene production
used on apples (Cunningham et al. 1985, Young (Baldwin et al. 1991a, 2000). Transgenic fruit
et al. 1996). Descriptive terms can be assigned to with antisense aminocyclopropanecarboxylic
the respective peaks on the GC chromatogram that acid (ACC) synthase (an enzyme in the ethylene
have odor activity (Acree 1993). The drawback biosynthetic pathway) had lowered levels of
to this method is that the interactive effects of many important flavor volatiles (Baldwin et al.
volatile compounds with each other and with 2000). Fruit with antisense pectinmethylesterase
sugars and acids, both chemically and in terms of (which demethylates pectin in cell walls) had
human perception, are eliminated. lowered levels of methanol, while those with
downregulated phytoene synthase (phytoene is a
precursor of carotenoids) had lowered levels of
Factors That Can Affect Flavor of Fruits carotenoid-derived volatiles (Baldwin et al. 2000).
and Vegetables
Effect of Preharvest Factors
Effect of Genetics on Flavor
Preharvest factors such as sunlight, water
Fruit and vegetable varieties differ in flavor based availability, fertilization, and chemical
on sensory and chemical analysis. “Charm” applications affect crop growth and can affect
analysis combines separation on a GC column internal quality characteristics of the harvested
with a sniff port to assign biological activity (odor product, including flavor. Preharvest treatment
activity) to individual aroma components as they with aminoethoxyvinylglycine (AVG) suppressed
are identified and quantified by GC (Cunningham volatile production in pears by 50%, which was
et al. 1985). This study with 40 cultivars showed reversed by ethylene exposure (Romani et al.
that apple aroma was not the result of the same 1983); and heavy rains prior to harvest dilute
compounds in every cultivar, although some flavor compounds in tomato. Fruit from tomato
common volatile compounds were important in all plants treated with increased levels of N and K
cultivars. fertilizer scored lower in sensory analysis and
showed increased levels of TA, SSC, and several
Important aroma-specific compounds for volatiles (Wright and Harris 1985). Preharvest
strawberry included ethyl butanoate, methyl mite control resulted in sweeter and more flavorful
butanoate, γ-decalactone, and 2-heptanone field grown strawberries than those receiving no
(Larsen et al. 1992). Strawberry cultivars differed treatments, according to a trained sensory panel
in flavor intensity and sweetness, according to (Podoski et al. 1997).
a trained sensory panel (Podoski et al. 1997).
Concentrations of several important compounds, Effect of Harvest Maturity
including α- and ß-ionones, were higher in wild
compared to cultivated raspberries (Rubus sp.). Horticultural crops should be harvested at optimal
In addition, numerous aroma compounds were eating quality, but practical considerations dictate
found only in wild berries, all of which may that they are harvested at a stage that minimizes
contribute to the stronger and more pleasant aroma physical damage during shipping and handling
of wild berries (Martin and MacLeod 1990). In and maximizes shelf-life. The climacteric stage at
tomato, the TA/SSC (Stevens et al. 1977) and harvest affected ester formation in apples (Fellman
132
et al. 1993). Harvest maturity affected both the apples (Fellman et al. 1993). However, when
sensory and chemical analysis of ripened tomato atmospheres induced anaerobic metabolism,
fruit (Maul et al. 1998). Tomatoes harvested at large concentrations of ethanol and acetaldehyde
the immature green stage resulted in ripened fruit accumulated. The altered synthesis of fruit
with lower volatile levels than mature-green- volatiles resulted in increased amounts of ethyl
harvested tomatoes. Harvest maturity also affected acetate and certain ethyl esters at the expense
consumer acceptability for mango and trained of others. Sensory analysis of CA-stored apples
descriptive panel ratings for sweetness, sourness, revealed that intensity of fruity and floral
and various aroma descriptors. Fruit harvested descriptors decreased after 10 weeks in CA, while
later were sweeter and less sour and generally had sourness and astringency were higher compared
more intense aroma characteristics (Baldwin et al. to apples stored in air. CA storage also increased
1999a). certain volatiles in tomato, compared to air-stored
fruit (Crouzet et al. 1986).
Effect of Postharvest Handling
Use of packaging and edible coatings can create
Various techniques are used to extend the shelf- a modified atmosphere (MA) with reduced O2
life of fruits and vegetables after harvest, to and elevated CO2 levels, similar to that of CA.
control postharvest decay, and to eliminate pests Lowering O2 and raising CO2 can maintain the
(quarantine treatments). These storage techniques quality of many fresh fruits and vegetables for
and treatments involve cold, heat, irradiation, extended periods. However, exposure of fresh
chemical applications, and different storage produce to O2 levels below their tolerance level
atmospheres. can increase anaerobic respiration and lead to the
development of off flavors. Use of edible coatings
Tomato fruit stored at 36, 41, 50, and 55 °F (2, 5, affects flavor and the level of volatile flavor
10, and 13 °C) had reduced levels of important compounds in citrus (Cohen et al. 1990), apple
volatiles and had less-ripe aroma and flavor (Saftner et al. 1999), and mango fruit (Baldwin
as well as more off flavors compared to fruit et al. 1999b). The coating barrier probably
stored at 68 °F (20 °C), as quantified by a trained induced anaerobic respiration and the synthesis of
descriptive panel (Maul et al. 2000). Subjection ethanol and acetaldehyde and entrapped volatiles,
of fruit to heat treatments for preconditioning and including ethanol and acetaldehyde (Baldwin et
decay control (McDonald et al. 1996) resulted in al. 1999b). In broccoli, sulfur-containing volatiles,
altered aroma volatile profiles. Heat treatment of including methanethiol and dimethyl disulfide,
apples to reduce physiological and pathological are produced in response to anaerobic conditions
disorders inhibited emission of volatile esters that can be created by MAP (Dan et al. 1997).
important to apple flavor (Fallik et al. 1997). Storing strawberries in MAP altered volatile
Levels of fructose and glucose, but not sucrose, profiles depending on conditions (CO2, mixed
decreased with increased storage time and storage gases, or air), enabling separation of the samples
temperature for muskmelon. However, sensory using multivariate statistics (Shamaila et al. 1992).
analysis did not find differences in flavor or Fruit treated with CO2 had the greatest change
sweetness between stored and freshly harvested in volatile levels. This was confirmed by another
melons (Cohen and Hicks 1986). study in which strawberry fruit stored in a CO2-
saturated atmosphere exhibited significant changes
CA storage altered flavor of apples, and, if in volatile levels and phenylalanine ammonia
prolonged, reduced volatile emission compared lyase (PAL) activity (Dourtoglou et al. 1995). The
to air-stored fruit, especially lipid-derived amino acid phenylalanine is the precursor to a
esters (Mattheis et al. 1995). Low-O2 storage number of volatiles through a pathway in which
decreased ester content and the enzymatic PAL is the key enzyme.
activity responsible for ester biosynthesis in
133
In addition to CA, other gaseous treatments of fruit ripen (Brady 1993). Aroma of peaches and
fruits and vegetables have been reported. Use nectarines is distinguished by the presence of
of ethylene to synchronize ripening has been γ- and δ-lactones (peach-like and coconut-like,
practiced for years on banana and tomato, as well respectively), although other esters and aldehydes
as for degreening of citrus. Ethylene gassing of contribute to peach flavor (Do et al. 1969, Crouzet
tomato fruit alters volatile levels (McDonald et al. 1990). γ-Lactones from C-5 to C-12,
et al. 1996). Treatment of apple fruit with δ-lactones, and unsaturated lactones represent
1-methylcyclopropene (1-MCP) and methyl more than 25% of the volatiles, with γ-lactone
jasmonate inhibited both ethylene production and being the second most abundant component after
production of many volatile alcohols and esters, benzaldehyde. γ-Undecalactone, although rarely
including the formation of esters from alcohols reported in natural extracts, has a distinct peach
(Fan and Mattheis 1999). Treatment of bananas odor. It has been named “peach aldehyde” and is
with 1-MCP also suppressed volatile production used in peach flavor formulations (Crouzet et al.
and composition, resulting in an increase in 1990). Ethyl hexanoate and 3-methylbutanoate,
alcohols and a decrease in related esters (Golding linalool, α-terpineol, 6-pentyl-α-pyrone (coconut
et al. 1999). Application of acetaldehyde and odor), and benzyl alcohol are also considered
ethanol vapors to blueberries, tomatoes, and pears important (Crouzet et al. 1990).
increased their sugar content, sugar-acid ratio, and
hedonic sensory rating (Paz et al. 1981). Small Fruits
Other chemical treatments of fresh produce Strawberry. In most berry fruits sucrose, glucose,
may also affect flavor. For example, pressure and fructose are present in roughly equivalent
infiltration of apples with calcium chloride concentrations (Manning 1993), and citrate is
transiently reduced levels of important flavor the major organic acid. More than 200 volatile
volatiles (Saftner et al. 1999). compounds have been identified in strawberry.
C-6 aldehydes such as hexanal and trans-2-
hexenal are found, as well as lipoxygenase and
Flavor of Some Popular Fruits and hydroperoxide lyase. Lipoxygenase acts on
Vegetables linolenic acid to form 13- and 9-hydroperoxides,
which are cleaved by hydroperoxide lyase to form
Apple. Sucrose is the major sugar in apples, hexanal and cis-3-hexenal. The cis-3-hexenal is
though it is slowly hydrolyzed to glucose and then isomerized to trans-2-hexenal (Perez et al.
fructose during latter ripening stages. The major 1999), as was reported for tomato (Galliard et al.
organic acid is malate, although some citrate 1977, Riley et al. 1996). 2,5-Dimethyl-4-hydroxy-
is also present (Knee 1993). Eleven aroma 3(2H)-furanone (furaneol) and its methyl ether
compounds contribute to apple aroma in most (mesifuran) are important aroma components in
of the 40 cultivars, while 27 other compounds both strawberry and tomato and are considered to
contributing to flavor where found only in certain be glycosidically bound in both fruits (Roscher et
genetic types (Cunningham et al. 1985). Loss of al. 1997). Of more than 100 volatile compounds
apple flavor after long-term CA storage is a major identified from strawberry, furaneol, ethyl
problem, probably due to the reduction of volatile hexanoate, and ethyl butanoate are considered to
synthesis during storage (Mattheis et al.1995). be the character-impact compounds (Zabetakis
and Holden 1997). Sensory analysis of strawberry
Peach (Prunus persica). The main sugar in juice showed that furaneol was positively related
peaches is sucrose, but cultivars differ greatly to fresh flavor and negatively related to off flavors,
in glucose:fructose:sorbitol ratios, which may while α-terpineol was inversely related to fresh
contribute to differences in flavor. The major flavor (Golaszewski et al. 1998).
organic acids are malate and citrate, with malate
levels declining and citrate levels increasing as
134
Raspberry (Rubus idaeus, R. ursinus). The include geraniol, citronellol, hydroxycitronellol,
main sugars in raspberry are sucrose, glucose, farnesol, and farnesyl acetate. Most volatiles are
and fructose, with citric as the major organic acid present below their threshold concentrations,
(Robbins and Fellman 1993). At least 200 volatile but hydroxycitronellol was described by
compounds have been identified in raspberry sensory panelists as blueberry-like. Rabbit-eye
(Honkanen and Hirvi 1990, Dourtoglou et al. blueberries (V. ashei) have a different aroma
1995). Impact flavor compounds for raspberry are than high-bush. Some aroma volatiles unique to
1-(p-hydroxyphenyl)-3-butanone, cis-3-hexenol, rabbit-eye blueberries include 1-penten-2-one,
α- and β-ionones, α-irone, and mesifurane. Other γ-terpinene, carveol, acetone, cis-caran-3-ol,
abundant volatiles include geraniol, nerol, and ecineralone, α-cedrene, sabinol, geranyl formate,
linalool among others (Paterson et al. 1993). The linalyl acetate, undecan-2-one, tridecan-2-one,
“raspberry ketone,” or character-impact volatile ethyl acetate, ethyl tetradecanoate, dimethyl
for raspberry, is 4-(4-hydroxyphenyl)-butan-2- octanedioate, toluene, p-cymene, and β-ionone,
one (Larsen and Poll 1990). It had the lowest among others (Honkanen and Hirvi 1990).
threshold (therefore the largest contribution to
flavor), followed by α-ionone, β-ionone, geraniol, Grape (Vitis). Glucose and fructose are the
linalool, and benzyl alcohol. Furaneol, linalool, predominant sugars in grapes, while tartaric and
and ethyl hexanoate were important general malic acids account for 90% of the TA (Kanellis
aroma compounds, while ethyl butanoate, methyl and Roubelakis-Angelakis 1993). Grapes show
butanoate, γ-decalactone, and 2-heptanone were an increase in free and glycosylated aroma
important cultivar-specific compounds (Larsen compounds at the end of ripening, after sugar
et al. 1992). The most potent flavor compounds accumulation has slowed (Coombe and McCarthy
identified using a retronasal aroma simulator 1997). This process is different from that of other
in raspberries were β-damascenone, diacetyl, berries and has been termed “engusting.” The
1-hexen-3-one, 1-nonen-3-one, 1-octen-3-one, and volatiles in wine grapes are the most complex
cis-3-hexenal (Roberts and Acree 1996). and are classified into five groups, of which the
first four have glycosylated forms: monoterpene
Blackberry (Rubus laciniata). Fresh (abundant in “floral” grapes), norisoprenoid,
blackberry fruit contain 245 aroma compounds benzenoid, aliphatic, and methoxypyrazine. The
(Georgilopoulos and Gallois 1987). The most accumulation of flavor volatiles occurs late in
abundant were heptan-2-ol, para-cymen-8-ol, the berry-ripening cycle, well after accumulation
heptan-2-one, hexanol, α-terpineol, pulegone, of sugar as observed in Muscat berries (Park et
octanol, isoborneol, mytenol, 4-terpineol, carvone, al. 1991). Different varieties have distinctive
elemincine, and nonanol. Though heptan-2-ol is an aroma character. For example, Muscat odor
important flavor compound with an intense fruit is mainly composed of monoterpenes such as
taste with herbaceous nuances, no single volatile linalool and geraniol (Webb 1981, Kanellis
was identified as blackberry-like (Marton and and Roubelakis-Angelakis 1993). Carbernet
MacLeod 1990). Some compounds in blackberry Sauvignon, a V. vinifera cultivar, contains
fruit and leaves are glycosidically bound, such methoxyisobutylpyrazine, which has a strong,
as benzyl alcohol, benzoic acid, 3-hydroxy-7,8- green-bell-pepper-like aroma (Webb 1981).
dihydro-β-ionol, and cis-3-hexenol among others Benzyl and 2-phenylethyl alcohols, ethers,
(Humpf and Schreier 1991). aldehydes, and hydrocarbons also contribute to
aroma. American grapes (V. labruscana and V.
Blueberry (Vaccinium). Blueberries have rotundifolia) are not suitable for wine production
glucose and fructose as their major soluble sugars because they possess what has been termed
and citric, malic, and quinic acids (Eck 1986). “foxy” and candy-like odors due to compounds
The odor-impact compounds for high-bush like methyl anthranylate, aminoacetophenone,
blueberry (Vaccinium myrtillus) are trans-2- furaneol, and methyl furaneol. ß-Phenylethanol,
hexenal, trans-2-hexenol, and linalool, but also with its rose-like odor, was found to be important
135
for muscadine (V. rotundifolia) aroma ( Flora and Tangerine (Citrus reticulata). Analysis of
Nakayama 1981). The V. vinifera grapes exhibit tangerine essence revealed 34 volatile compounds
a mild aroma that is more desirable for wine that were odor contributors. However, no one
production (Shure and Acree 1995). compound was found to have a characteristic
tangerine odor (Moshonas and Shaw 1972).
Banana (Musa). Sucrose is the predominant Later studies suggested that the compounds
sugar in banana initially, but as ripening proceeds thymol and methyl-N-methylanthranilate
glucose and fructose accumulate. Malic, citric, (dimethylanthranilate) are odor-impact
and oxalic acids are the predominant organic compounds for this fruit but that they are modified
acids, with the astringent taste of unripe bananas by the presence of monoterpene hydrocarbons.
being attributed in part to oxalate levels (Seymour Nevertheless, dimethylanthranilate is the most
1993). The characteristic aroma of bananas potent flavor component (Shaw and Wilson 1980).
arises from a complex mixture of compounds
including short-chain fatty acids such as acetates, Grapefruit (Citrus paradisi). At least 126
butanoates, and 3-methylbutyl esters. Recently, volatile components have been identified in
nonvolatile glycoside precursors were shown grapefruit (Demole et al. 1982). Nootkatone and
to release glycosidically bound volatiles from 1-p-menthene-8-thiol may be key aroma-impact
banana pulp by ß-glucosidase, including decan- compounds for grapefruit (Demole et al. 1982),
1-ol, 2-phenylethanol, 3-oxy-pentanoic acid, although aldehydes and esters are also important
3-methylbutanoic acid, and benzoic acid (Perez (Shaw and Wilson 1980).
et al. 1997). Esters account for about 70% of the
volatile compounds and acetates and butyrates Mango (Mangifera indica). The major sugars
predominate (Seymour 1993). 3-Methylbutyl in mango are glucose, fructose, and sucrose,
acetate, however, is considered to dominate with sucrose predominating. The major acids
banana flavor as the key odor-impact volatile are citric, malic, and sometimes tartaric at 0.1%
(Berger 1991), along with butanoate and to 0.4% TA (Nairain et al. 1997, Baldwin et al.
3-methylbutanoate (Engel et al. 1990). Unusual 1999b) and 10 to 16 SSC (Baldwin et al. 1999b).
phenol derivatives eugenol, 5-methoxyeugenol, Mango varieties differ in amount and type of
eugenol-methylether, and elemicin contribute volatile compounds present (more than 150
background notes for the full-bodied mellow compounds identified), often depending on area of
aroma of ripe bananas (Engle et al. 1990). production. Asian mangoes have more oxygenated
volatile compounds such as esters, furanones,
Citrus Fruits and lactones, giving some varieties pineapple- or
peach-like aromas (Narain et al. 1997), while
Sweet Orange (Citrus sinensis). The major sugar western mangoes that are hybrids of Asian stock
in most citrus types is sucrose, with varying levels have higher levels of certain hydrocarbons such as
of glucose and fructose. The major acid is citrate. 3-carene (MacLeod and de Troconis 1982, Wilson
Typical orange aroma is attributed to alcohols, et al. 1986, Narain et al. 1997).
aldehydes, esters, hydrocarbons, ketones, and
other components, of which more than 200 have Pineapple (Ananas comosus). Besides banana
been identified. Of these, esters and aldehydes are and possibly mango, pineapple is the most popular
the primary contributors, followed by alcohols, fruit from the tropics. SSC can range from 11°
ketones, and hydrocarbons (Bruemmer 1975). to 17° Brix, and the major sugars are glucose,
There is no single impact compound for orange. fructose, and sucrose, with sucrose predominating
However, octanal, decanal, nonanal, dodecanal, (Salunkhe and Desai 1984, Shukor et al. 1998).
ethylbutyrate, and limonene are likely contributors The major acids are citrate and malate with
to flavor (Shaw and Wilson 1980, Shaw 1991). about 0.1 to 0.6% titratable acidity (Salunkhe
and Desai 1984, Shukor et al. 1998). More than
120 volatiles have been identified in green and
136
ripened pineapples with esters dominating at aroma of a fresh ripe tomato. Of these, cis-3-
more than 80% of the total volatiles (Shukor hexenal and β-ionone have the highest odor
et al. 1998). Contributing aroma volatiles, units, and 2-isobutylthiazole is unique to tomato.
based on odor thresholds, show that pineapple Furaneol has an odor threshold indicating it may
aroma is also dominated by esters such as ethyl contribute to flavor (Buttery et al. 1995). Volatile
2-methylbutanoate, ethyl acetate, ethyl hexanoate, production occurs at the same time that ethylene
ethyl butanoate, methyl heptanoate, and others. increase, carotenoid synthesis, and chlorophyll
breakdown occur (Baldwin et al. 1991a). Enzymes
Melons (Cucumis melo). Sucrose is the principal important in volatile synthesis from lipids include
sugar in most melon types, although high levels lipoxygenase, hydroperoxide lyase (hydroperoxy
of fructose may be present in some watermelon cleavage), and alcohol dehydrogenase (Galliard et
cultivars. Melons contain citrate and malate, al. 1977, Riley et al. 1996). Amino acid precursors
or only malate in watermelon (Seymour and include alanine, isoleucine, leucine, phenylalanine,
McGlasson 1993). Ethyl 2-methylbutanoate and valine (Buttery and Ling 1993). Glycosides
and methyl-2-methylpropanoate are among are also precursors to some volatiles (Krammer et
the most significant contributors to flavor of al. 1994). Furaneol is also reported to be important
muskmelon cultivar ‘Makdimon,’ one of the C. (seems it is found in every fruit). Genetically
mello reticulatus cultivars that exhibit strong engineered fruit with down- or up-regulated
characteristic aromas. Muskmelon and watermelon alcohol dehydrogenase expression exhibited
also have cis-non6-enal and cis,cis-nona-3,6,- altered levels of some related volatiles (Speirs et
dien-1-ol, respectively. The former has a strong al. 1998).
melon-like aroma, while the latter is reminiscent
of watermelon rind. 4-Oxononanal and 2-hydroxy-
5-pentyltetrahydrofuran have fruity and green
odors and contribute to watermelon aroma. The
volatile cis-non6-enyl acetate has a pleasant
honeydew-melon-like aroma (Engle et al. 1990).
Other varieties have ethyl-2-methylpropanoate,
2-methylbutyl acetate, 2-methylpropyl acetate,
and the thioether esters (Wyllie et al. 1995).
137
Table 2. Some important or abundant flavor compounds in selected fruits and vegetables
____________________________________________________________________________________________
Peach
Brady 1993 sucrose malic benzaldehyde
Crouzet et al. 1990 glucose citric benzyl alcohol
Do et al. 1969 fructose nonanol
sorbitol
linalool
ethyl hexanoate
3-methylbutanoate
α-terpineol
γ-hexalactone
δ-decalactone
γ-undecalactone
δ-undecalactone
γ-dodecalactone
δ-dodecalactone
α-pyrone
6-pentyl-α-pyrone
Strawberry
Golaszewski et al. 1998 sucrose citric hexanal
Honkanen et al. 1980 glucose cis-3-hexanal
Manning 1993 fructose trans-2-hexanal
Perez et al.1999 furaneol
Roscher et al. 1997 mesifuran
Zabetakis and Holden 1997 ethyl hexanoate
ethyl butanoate
methyl butanoate
ethyl-2-methyl propanoate
138
Table 2. Some important or abundant flavor compounds in selected fruits and vegetables
—Continued
____________________________________________________________________________________________
Fruit and references Major Major Important aroma
sugars acids compounds
____________________________________________________________________________________________
Raspberry
Dourtoglou et al. 1995 sucrose citric H-(4-hydroxyphenyl-butan-2-one)
Honkanen and Hirvi 1990 glucose (raspberry ketone)
Larsen and Poll 1990 fructose α-ionone
Larsen et al. 1992 β-ionone
Paterson et al. 1993 geraniollinalool
Robbins and Fellman 1993 benzyl alcohol
Roberts and Acree 1996 ethyl hexanoate
ethyl butanoate
methyl butanoate
γ-decalactone
2-heptanone
cis-3-hexanal
β-damascenone
Muscadine β-phenylethanol
(V. rotundifolia) butyl alcohol
hexyl alcohol
hexanal
trans-2-hexenal
isoamyl alcohol
acetaldehyde
isobutyraldehyde
ethyl acetate
ethyl propionate
butyl acetate
propyl acetate
2-methylbutanol
139
Table 2. Some important or abundant flavor compounds in selected fruits and vegetables
—Continued
____________________________________________________________________________________________
Fruit and references Major Major Important aroma
sugars acids compounds
____________________________________________________________________________________________
Muscat varieties linalool
(V. vinifera) geraniol
methoxyisobutylpyrazine
Banana
Berger 1991 sucrose malic decan-1-ol
Engel et al. 1990 glucose citric 2-phenylethanol
Perez et al. 1997 fructose oxalic 3-oxy-pentanoic acid
Seymour 1993 3-methylbutanoic acid
3-methylbutyl acetate
butanoate
3-methylbutanoate
eugenol
5-methoxyeugenol
eugenol-methylether
elemicin
Sweet orange
Bruemmer 1975 sucrose citric geranial
Shaw 1991 glucose neral acetaldehyde
Shaw and Wilson 1980
fructose decanal
octanal
nonanal
ethyl acetate
ethyl propionate
ethyl butanoate
methyl butanoate
ethyl-2-methyl butanoate
ethyl-3-hydroxy hexanoate
linalool
α-terpineol
limonene
myrcene
α-pinene
valencene
Tangerine
Moshonas and Shaw 1972 sucrose citric acetaldehyde
Shaw and Wilson 1980 glucose decanal
fructose octanal
dimethyl anthranilate
thymol
α-sinensal
γ-terpinene
β-pinene
140
Table 2. Some important or abundant flavor compounds in selected fruits and vegetables
—Continued
____________________________________________________________________________________________
Fruit and references Major Major Important aroma
sugars acids compounds
____________________________________________________________________________________________
Grapefruit
Demole et al. 1982 sucrose citric acetaldehyde
Shaw and Wilson 1980 glucose decanal
ethyl acetate
methyl butanoate
ethyl butanoate
1-p-menthene-8-thiol
nootkatone
limonene
naringin
Mango
Baldwin et al. 1999 sucrose citric ethyl butanoate
MacLeod and de Troconis 1982 glucose malic ethyl-2-butanoate
Nairain et al. 1997 fructose hexanal
Wilson et al. 1986 cis-3-hexanal
trans-2-hexanal
γ-octalactone
γ-dodecalactone
furaneol
α-pinene
β-pinene
3-carene
myrcene
limonene
p-cymene
terpinolene
α-Copaene
caryophyllene
141
Table 2. Some important or abundant flavor compounds in selected fruits and vegetables
—Continued
____________________________________________________________________________________________
Fruit and references Major Major Important aroma
sugars acids compounds
____________________________________________________________________________________________
isopropyl acetate
ethyl propanoate
ethyl isobutanoate
propyl acetate
butyl acetate
methyl-2-methylbutanoate
ethyl butanoate
2-methylpropanoate
2-methylbutyl acetate
2-methylpropyl acetate
methyl (methylthio) acetate
ethyl (methylthio) acetate
ethyl (methylthio) propanoate
Tomato
Baldwin et al. 1991a,b glucose citric hexanal
Buttery 1993 fructose malic trans-2-hexenal
Buttery and Ling 1993 cis-3-hexenal
Buttery et al. 1995, 1989 cis-3-hexenol
De Bruyn et al. 1971 β-ionone
Hobson and Grierson 1993 β-damascenone
1-penten-3-one
3-methylbutanal
3-methylbutanol
2-isobutylthiazole1-nitro-
phenyl-ethane
trans-2-heptenal
phenylacetaldehyde
6-methyl-5-hepten-2-one
methyl salicylate
geranylacetone
____________________________________________________________________________________________
142
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Food Safety human consumption.
James R. Gorny and Devon Zagory Systems that ensure safety and wholesomeness of
fruits and vegetables during postharvest handling
Gorny was with the International Fresh-cut and fresh-cut processing fall into four prevention
Produce Association, Alexandria, VA. He is now programs: Good Agricultural Practices (GAPs),
with the Produce Marketing Association, Newark, Good Manufacturing Practices (GMPs), Sanitation
DE. Zagory was with Food Safety & Quality Procedures, and Hazard Analysis Critical Control
Programs, Davis Fresh Technologies, Davis, CA. Points (HACCPs).
He is now with NSF Agriculture, Davis, CA.
The greatest risk to human health from
consumption of uncooked produce is from
Introduction pathogenic microorganisms. Raw agricultural
products, such as fresh produce, should
Data from the U.S. Center for Disease Control be expected to harbor a wide variety of
and Prevention (CDC) between 1973 and 1987 microorganisms including the occasional
indicate that there were 3,699 foodborne illness pathogen. A vigorous population of nonpathogenic
outbreaks in the United States. Only 2% were bacteria can be an excellent barrier to prevent
associated with fruits and vegetables, and most the growth of pathogens, should they be present.
of those were due to improper home canning. Nonpathogenic bacteria also act as indicators
In general, produce is a low-risk food, and it is of temperature abuse and age by spoiling the
unlikely that one will become ill from eating raw product. In the absence of spoilage, high levels
fruits or vegetables. But a small risk does exist, of pathogens may occur, and the item may be
and it is incumbent on all of those involved in consumed because it is not perceived as spoiled.
the production and distribution of fresh produce There are four groups of human pathogens
to work to minimize those risks. Safety is the associated with fresh produce:
perception of acceptable risk, and if no risk is • Soil-associated pathogenic bacteria
acceptable, then nothing can ever truly be safe. (Clostridium botulinum and Listeria
Many consumers feel that food products should moncytogenes)
have no risk associated with their consumption. • Fecal-associated pathogenic bacteria
Unfortunately, the reality is that reducing the
(Salmonella spp., Shigella spp., E. coli
risk of foodborne illness from consumption of
O157:H7, and others)
fresh fruits and vegetables to absolute zero is an
• Pathogenic parasites (Cryptosporidium and
impossible task. It should also be kept in mind that
Cyclospora)
the health benefits derived from eating at least 5
• Pathogenic viruses (hepatitis, norwalk virus,
servings of fresh fruits and vegetables daily far
outweigh the very small probability of contracting and others)
a foodborne illness.
Many of these pathogens are spread from humans
Fruits and vegetables are unique foods, since or domestic animals to food to humans. Fruits and
they are often consumed raw or with minimal vegetables may become contaminated by infected
preparation. To date, there have been no effective field workers, food preparers, consumers, cross-
intervention strategies developed that can contamination, use of contaminated irrigation
completely eliminate food safety risks associated water, use of inadequately composted manure, or
with consumption of uncooked produce. contact with contaminated soil. To minimize risks,
Therefore, preventing contamination with human growers should implement practices outlined
pathogens, dangerous levels of chemical residues, in the “Guide to Minimize Microbial Food
or physical contaminants is the only way to ensure Safety Hazards for Fresh Fruits and Vegetables”
that these foods are wholesome and safe for published by the Center for Food Safety and
149
Applied Nutrition, Food and Drug Administration Table 1. Probability that a given number
(FDA 1998). This publication outlines Good of samples will fail to detect microbial
Agricultural Practices (GAPs) which, when contaminants at specified contamination
followed, can significantly reduce the risk of levels
microbial hazards in produce. Growers should be _____________________________________
aware that agricultural practices that may have Percent Number of Samples Analyzed
been acceptable in years past may no longer Contaminated 5 10 15 20 30 60
be acceptable. In addition, fresh-cut processors -------------------%----------------
should adhere to Good Manufacturing Practices 10.0 41 65 79 88 96 >99
(GMPs) 21 [CFR 100-169] to appropriately 5.0 33 40 54 64 79 95
manage food safety risks during processing. Food 2.0 10 18 26 33 45 70
handlers and consumers must act responsibly as 1.0 5 10 14 18 26 45
they are the final link in the food safety chain. 0.1 1 1 2 2 3 6
_____________________________________
Prevention of contamination is the only way to Source: U.S. Food and Drug Administration
minimize true food safety risks and ensure food
safety. Microbial testing cannot guarantee the Has the Problem Gotten Worse?
absence of pathogens on fresh produce and, in
fact, is unlikely to detect pathogens even when Scientists continue to discover new
they are present. For example, if 5 fruit in a microorganisms that cause foodborne illness,
given lot of 100 individual fruit are harboring and recent advances in diagnostics allow more
pathogens (5% contamination rate), how many rapid detection of smaller numbers of pathogens
fruit would have to be sampled to be 95% sure on foods. Detection methods for pathogenic
that one of the infected fruit was found? Table 1 microorganisms are faster and more sensitive,
shows that at 5% contamination rates, it would be allowing investigators to better identify causes of
necessary to test 60 fruit to have a 95% chance of outbreaks. In recent years, fresh produce sourcing
finding the pathogen. It is surely not practical to has undergone significant changes, and centralized
test 60 out of every 100 fruits or vegetables. Yet local production has been replaced with
testing fewer fruits results in a high likelihood worldwide sourcing. Agricultural practices and
that pathogens will be missed, even when they hygienic conditions vary greatly among growing
are present. For this reason, negative results from regions around the world, and increased global
product pathogen testing have little value and sourcing increases consumers exposure to diverse
can be misleading. Microbial testing can be an endemic microflora carried on fresh fruits and
effective tool, but sampling the finished product is vegetables. Also, global sourcing means longer
not an efficient, cost-effective approach. Sampling transportation and handling, giving pathogenic
potential sources of contamination—such as microorganisms additional time to proliferate and
irrigation water, cooling and process water, and reach levels which can cause illness. Population
food contact surfaces—and monitoring employee demographics in North America have shifted, with
hygiene practices are more effective in preventing a greater number of individuals that are older or
spread of human pathogens. who have compromised immune systems. They
are at greater risk from foodborne illness, and the
consequences of exposure can be deadly. All of
these circumstances have resulted in increased
foodborne illness awareness.
150
Reported Food Borne Illness Outbreaks In the United This is a problem since some pathogens, such as
States Due to Fruits and Vegetables
E. coli O157:H7, have an infectious dose of as
30
few as 10 to 100 viable cells. To date, there are
25
No. Of Outbreaks
20
no wash-water treatments that can completely
15
eliminate human pathogens from fresh produce.
10
Product wash-water, if not properly sanitized, can
5 become a source of microbiological contamination
0 for every piece of product that passes through
74 76 78 80 82 84 86 88 90 92 94 96 98
Two Year Interval
that water. It is a widespread misconception
that chlorinated wash-water cleans or sterilizes
Figure 1. Increasing number of foodborne illness outbreaks
produce as it is washed. Chlorinated wash-water
associated with fresh produce in the United States. Source: does little more to clean produce than potable,
CDC Food Borne Outbreak Surveillance System. nonchlorinated water. Chlorine does sanitize
wash-water and maintains a low microbiological
Intervention Strategies count in the water. In this way the water does not
become a reservoir for mold spores and bacteria to
Washing produce before preparation or infest produce.
consumption is recommended but does not
guarantee that fresh produce is pathogen-free. Sodium or calcium hypochlorite is most
Studies have demonstrated that washing produce commonly used in produce wash-water. The
in cold chlorinated water will reduce microbial antimicrobial activity of these compounds depends
populations by 2 or 3 logs (100- to 1000-fold), but on the amount of hypochlorous acid (HOCl)
sterility is not achieved, because microorganisms present in the water. This, in turn, depends on the
adhere to surfaces of produce and may be present pH of the water, the amount of organic material
in microscopic nooks and crannies on the surface in the water, and, to some extent, the temperature
of produce (Zhuang et al. 1995). of the water. Above pH 7.5, very little chlorine
occurs as active hypochlorous acid, but rather as
5 inactive hypochlorite (OCl-). Therefore, the wash-
water pH should be kept between 6.0 and 7.5 to
4 ensure chlorine activity. If the pH falls below 6.0,
S. montevideo
Log CFU/cm 2
0
0 60 110 210 320
Chlorine (ppm)
151
Effect of pH on Chlorine Dissociation Products
% Chlorine present as HOCl consumer resistance to the use of irradiation for
100 foods. Irradiation with a gamma source, such as
cobalt 60, has been studied by many researchers.
80
In the 30 years preceding 1983, more than a
thousand published reports addressed irradiation
or OCl-
60
of fruits and vegetables (Kader and Heintz 1983).
40
The accumulated data suggest that irradiation may
20 have some applications for disinfestation of fruits
and vegetables but that irradiation alone will not
0 resolve most microbiological issues. Different
0 1 2 3 4 5 6 7 8 9 10 organisms vary in their sensitivity to ionizing
pH HOCl radiation and many microbes will not be killed at
OCl-
the maximum allowable dose of 1 kGy (Brackett
Figure 3. Effect of pH on chlorine dissociation products.
1987).
An effective wash-water sanitation system is Killing microbes with irradiation occurs when the
becoming a necessity in the produce industry due irradiated energy interacts with water in microbial
to increased concerns with safety of fresh produce. cells. Reactive chemicals are created that damage
Since water can be a source of contamination if the cells’ genetic material, or DNA. The ability of
the water itself becomes contaminated, the ability irradiation to kill a particular microbe is measured
to ensure clean water is an essential element of a as the “D-value,” the amount of energy needed
food safety program. Understanding how different to kill 90% of the cells of the microbe. Thus, a
sanitizers work and how they are measured and dosage of 2D would kill 99% of the cells, 3D
monitored is an important element in operating kills 99.9% and so on. Of course, the D-value will
a food safety system in an effective and cost- differ for different microorganisms.
efficient manner (See table 2.)
Insect pests and some parasites (Cyclospora,
Irradiation and Cold Pasteurization Cryptosporidium, etc.) have a relatively large
amount of water and DNA in their cells and so are
Use of nonthermal irradiation, often called cold easily killed by irradiation. D-values for gamma
pasteurization, has been advocated as a means to irradiation of 0.1 kGy are typical. Thus, a dosage
eliminate human pathogens from produce, similar of 0.5 kGy would give a 5-log reduction. Bacteria
to current allowable practices in the meat and (E. coli, Salmonella, Listeria, etc.) have less DNA
poultry industry. To date this strategy has been and are more resistant to irradiation. D-values
ineffective for a number of reasons, including the of 0.3 to 0.7 kGy are typical, depending on the
expense of irradiating produce, a lack of facilities bacterium. Thus, it would require 1.5 to 3.5 kGy
to treat produce, the damage susceptibility of to achieve a 5-log reduction of bacteria. At this
many produce items to irradiation, and perceived time, the maximum allowable dosage for treating
153
biosolid wastes should be closely managed soil for up to 250 days (Suslow 1999). Proper
to minimize the potential for microbial composting via thermal treatment reduces the
contamination of fresh produce. risk of potential foodborne illness. However, the
• Worker hygiene and sanitation practices persistence of many human pathogens in untreated
during production, harvesting, sorting, agricultural soils is unknown. Use of inorganic
packing, and transport play a critical role fertilizers, which have been certified to be free of
in minimizing the potential for microbial heavy metals and other chemical contaminants, is
contamination of fresh produce. recommended.
• All applicable local, State, and Federal laws
and regulations, or corresponding or similar Irrigation Water. Irrigation water is another
laws, regulations, or standards for operators potential vector by which contaminants may be
outside the United States for agricultural brought into contact with fruits and vegetables.
practices should be followed. Deep-well water is less likely to be contaminated
• Accountability at all levels of the with human pathogens than surface water
agricultural environment (farms, packing supplies. However, all irrigation water sources
facility, distribution center, and transport should be periodically tested for contamination
by pesticides and human pathogens. The
operation) is important to a successful food
presence of E. coli is a useful indicator for fecal
safety program. There must be qualified
contamination and possible presence of human
personnel and effective monitoring to ensure
pathogens. Inexpensive test kits for generic E.
that all elements of the program function
coli are available from several vendors. Overhead
correctly and to help track produce back
irrigation systems are more likely than flood,
through the distribution channels to the furrow, or drip irrigation to spread contamination
producer. since contaminated water is applied directly to
the edible portions of fruits and vegetables. Water
Land Use. The safety of food grown on any given used to mix or spray agricultural chemicals must
parcel of land is influenced not only by the current be confirmed to be free of pathogens before use.
agricultural practices but also by former land use
practices. Heavy metals and pesticide residues Pesticide Use. All pesticide use should be
may persist in soils for long periods of time. done in strict accordance with manufacturer’s
Soil should be tested to ensure that dangerously recommendations as well as Federal, State, and
high levels of these compounds are not present. local ordinances. Monitoring and documentation
Former land use should also be investigated and of proper pesticide use should be done to prevent
documented to ensure that the production land was unsafe or illegal residues from contaminating
not formerly used for hazardous waste disposal or fruits and vegetables. All pesticide applications
for industrial purposes that may have left behind should be documented, and proper records of
toxic residues. If production land was previously application should be available and reviewed by
used for agricultural purposes, pesticide use management on a regular basis. Appropriately
records should be reviewed to ensure that proper trained and licensed individuals should perform
pesticide management practices were followed. pesticide use recommendations and applications.
Production acreage should not have recently been
used as a feedlot or for animal grazing, because Harvest Operations. During harvest operations,
fecal contamination of the soil may persist. field personnel may contaminate fresh fruits and
vegetables by simply touching them with an
Fertilizers. Improperly composted or unclean hand or knife blade. Portable field latrines
uncomposted manure is a potential source of equipped with hand-washing stations must be
human pathogens. Human pathogens may persist available and used by all harvest crew members.
in animal manure for weeks or even months. Training, monitoring, and enforcement of field
E. coli O157:H7 has been found to survive in worker hygiene practices, such as washing hands
uncomposted dairy manure incorporated into
154
after using the bathroom, are necessary to reduce a food-contact-surface sanitizer. Sanitizers should
the risk of human pathogen contamination. Once be used only after thorough cleaning with abrasion
harvested, produce should not be placed on soil to remove organic material such as dirt or plant
before being placed in clean and sanitary field materials. Steam or high-pressure water should be
containers. Field harvesting tools should be clean used with care as it may create bacterial aerosols
and sanitary and should not be placed directly and actually help spread contamination throughout
in contact with soil. Field containers should be the packinghouse facility.
cleaned and sanitized on a regular basis and
should be kept free of contaminants such as mud, Cold Storage Facilities. Cold storage facilities,
industrial lubricants, metal fasteners, or splinters. and in particular refrigeration coils, refrigeration
Plastic bins and containers are recommended as drip pans, forced-air cooling fans, drain tiles,
they are easier to clean and sanitize than wooden walls, and floors, should be cleaned and sanitized
ones. on a regular basis. The human pathogen Listeria
monocytogenes can multiply at refrigerated
Sanitary Postharvest Handling of Produce. temperatures in moist conditions and may
Depending on the commodity, produce may be contaminate produce if condensation from
field-packed in containers that will go all the way refrigeration units or ceilings drips on to the
to the destination market, or it may be temporarily produce. A common environmental pathogen, L.
placed in bulk bins, baskets, or bags that will monocytogenes, may get on walls, in drains, and
be transported to a packing shed. Employees, into cooling systems. Comprehensive sanitation
equipment, cold storage facilities, packaging programs that target these areas are important in
materials, and any water that will contact the preventing establishment of this pathogen.
harvested produce must be kept clean and sanitary
to prevent contamination. Packaging Materials. All packaging materials
should be made of food-contact-grade materials
Employee Hygiene. Gloves, hairnets, and clean to ensure that toxic compounds in the packaging
smocks are commonly worn by packing house materials do not leach out of the package and
employees in export-oriented packing sheds. The into the produce. Toxic chemical residues may
cleanliness and personnel hygiene of employees be present in some packaging materials due to
handling produce at all stages of production and use of recycled base materials. Packages, such
handling must be managed to minimize the risk of as boxes and plastic bags, should be stored in
contamination. Adequate bathroom facilities and an enclosed storage area to protect them from
hand-washing stations must be provided and used insects, rodents, dust, dirt, and other potential
properly to prevent contamination of produce by sources of contamination. Plastic field bins and
packing house employees. Shoe- or boot-cleaning totes are preferred to wooden containers since
stations may also be in place to reduce the amount plastic surfaces are more amenable to cleaning
of field dirt and contamination that enters the and sanitizing. Field bins should be cleaned and
packing shed from field operations. Employee sanitized after every use. Wooden containers
training in sanitary food handling practices or field totes are almost impossible to sanitize
should be done when an employee is hired, before since they have a porous surface and wooden
beginning work, and on a regular basis thereafter. or metal fasteners, such as nails from wooden
All training should be documented and kept on containers, may accidentally be introduced into
file. produce. Cardboard field bins, if reused, should
be inspected for cleanliness and lined with clean
Equipment. Food contact surfaces on conveyor plastic bags before reuse to prevent risk of cross-
belts, dump tanks, etc. should be cleaned and contamination.
sanitized on a regularly scheduled basis with
approved cleaning compounds. A 200 ppm (200
µL L-1) NaOCl solution (bleach) is an example of
155
Produce Wash-Water and Hydrocooling Water. (if any) are remote or nonexistent. Products are
All water that comes in contact with produce for recalled because of adulteration (filth in produce
washing, hydrocooling, or vacuum cooling must relating to aesthetic quality) or misbranding (label
be potable. To achieve this, water should contain violation), and the product does not involve a
between 2 and 7 ppm (µL L-1) free chlorine and health hazard. Every food provider should develop
have a pH between 6 and 7. Total chlorine up to a recall or trace-back plan and an organizational
200 µL L-1 is allowed by law, though 50 to 100 structure that enable it to remove product from the
ppm (µL L-1) is usually sufficient if the pH of market in a rapid and efficient manner.
the water is between 6 and 7. Alternatively, an
oxidation-reduction potential greater than 650 Good Manufacturing Practices (GMPs)
mV using any oxidative sanitizer will ensure
that bacteria in the water are killed on contact. GMPs ensure that food for human consumption
Chlorine use prevents cross-contamination of is safe and has been prepared, packed, and held
produce in the washing or hydrocooling system under sanitary conditions. GMPs are mandatory
but it will not sterilize the produce. Rinsing for the fresh-cut produce industry, but not
produce with potable water will reduce the mandatory for packinghouse or field operations
number of microorganisms present on the produce that simply handle whole produce. However,
but will not remove all bacteria. though GMPs are not mandatory for packing
sheds, they are mostly good common sense
Refrigerated Transport. Produce is best shipped and are recommended for all produce-handling
in temperature-controlled refrigerated trucks. facilities.
Maintaining most perishables below 5 °C (41
°F), except for tropical fruit, will extend shelf- The Code of Federal Regulations describes the
life and significantly reduce the growth rate conditions under which food must be processed
of microbes, including human pathogens. Cut and handled. The regulations cover general
produce, including tropical fruits, should always provisions, buildings and facilities, equipment,
be stored below 5 °C (41°F). Trucks used during production and process controls, and defect action
transportation should be cleaned and sanitized levels. Many of the GMPs are simple good sense,
on a regular basis. Trucks that have been used to such as washing hands after using the restrooms
transport live animals, animal products, or toxic and wearing hairnets when working with food.
materials should not be used to transport produce. Unlike GAPs, GMPs are regulations and have
the weight of law: A food processor must comply
Recall and Traceback Plans. Recall of product is with GMPs. Copies of the Current GMPs (CGMP)
the last line of defense in a food safety emergency. can be obtained by subscribing to the Federal
This action may be initiated by the company, Register or by ordering 21 CFR 100-169 (Code
performed on a voluntary basis, or done at the of Federal Regulations, Title 21, Food and Drugs,
request of the FDA because of a suspected Pt. 100-169). Submit a check or money order to
hazard in the product. The FDA has defined three Superintendent of Documents, U.S. Government
recall classifications and FDA actions. Class I Printing Office, Washington, DC 20402; or
is an emergency situation involving removal of telephone the Government Printing Office at
products from the market that could lead to an 202-783-3238 to order by credit card.
immediate or long-term life-threatening situation
and involve a direct cause-effect relationship; for Personnel GMPs. Personnel working in food
example, C. botulinum in the product. Class II is a processing plants or packinghouses can be a
priority situation in which the consequences may significant source of food contamination. This
be immediate or long term and may be potentially includes production employees, maintenance
life threatening or hazardous to health; for employees, supervisors, and management. It is the
example, Salmonella in food. Class III is a routine responsibility of processing plant management to
situation in which life-threatening consequences educate and train all food handlers about sanitary
156
handling of foods. Employees experiencing corrosion. Overhead pipes, ducts, and fixtures
diarrhea, vomiting, open skin sores, boils, fever, should not be suspended over work areas, and
or disease should report these symptoms to their horizontal surfaces of these items should be
supervisor and should not be allowed to work with minimized to reduce accumulation of dust and
food products. All food handlers should have clean water condensation. Where possible, overhead
outer garments or frocks and thoroughly wash structures should be hidden above a false ceiling,
their hands before entering a food processing area, and all hoses, pipes and electrical conduits should
especially after toilet use. No jewelry (earrings, descend vertically from the ceiling so as to not
pendants, rings, etc.), pens, or wrist watches provide horizontal surfaces for accumulation of
should be allowed in food processing areas, filth. Adequate lighting should be provided and
because these items may fall into food products all light bulbs should be covered to ensure that
unnoticed. Intact, clean, and sanitary gloves and broken glass cannot contaminate food products.
hair restraints should be used by all personnel in
food handling areas. All water (rinse, flume, cleaning, ice, etc.) used
in food processing must be of proper sanitary
Physical Plant and Grounds. Food processing quality. Plumbing should be of adequate size and
design to handle the amount of product being
plants and produce packing houses should be
processed. Produce handling environments are
constructed so as to segregate food handling
usually wet; therefore, sloping floors with drains
activities from the outside environment. The
should be present to remove excess water from
physical building itself should have no openings
the processing area. Sanitary sewer lines should
or gaps which could allow entrance by rodents,
be separate from floor drains to ensure that cross-
insects, or birds. Surrounding grounds should be contamination of the processing area from sewage
free of clutter such as equipment, litter, waste, backflow does not occur.
refuse, or animal feces. No unpaved or dusty roads
should be adjacent to food handling facilities, An adequate number of toilets and hand-washing
and areas surrounding the structure should be stations should be available to accommodate
adequately drained so that no standing or pooled all employees. Restroom facilities should not
water is present. Vegetation surrounding the open into processing areas. Hot running potable
processing plant should be kept down to prevent water, soap, and hand towels should be available
the formation of breeding grounds for rodents. at all times. Signs should be posted to instruct
Rodent traps should be placed on the outside employees to wash their hands after using the
perimeter of the grounds and be inspected and restroom. Employee frocks, gloves, and knives
serviced regularly. should never be taken into the restrooms, and
adequate storage space should be made available
Plant Construction and Design. The most directly outside the restroom door for temporary
important aspect of sanitary food plant and storage of these items. Heating, ventilation, and
produce packinghouse design is sufficient space air-conditioning (HVAC) systems may feature
for sanitary operations. Processing areas should filtered positive air pressure in processing plants
be designed for easy cleaning and sanitation. because of the potential for airborne pathogen
Floors, walls, and ceilings should be made of a contamination. HVAC units should blow air along
cleanable, noncorrosive, nonabsorbent material the ceiling and down the walls to keep the walls
and be in good repair. Floors should have rounded dry and free of condensation.
corner joints where they meet the wall to allow
for easy cleaning. Processing plant floors should Sanitation Procedures. Cleaning and sanitation
be constructed of sealed concrete or tile to are some of the most important programs in any
withstand the physical and chemical abuses from food processing plant or packing shed. Cleaning is
machinery and cleaning chemicals. Equipment the removal, through physical action, of debris and
should be constructed of stainless steel to prevent filth. Sanitation is the application of antimicrobial
157
compounds. Sanitation cannot be effective until prepared for specific pieces of equipment that
surfaces are cleaned. Regular and scheduled are cleaned on a regular basis. This ensures that
equipment cleaning and sanitizing ensures that the equipment is cleaned properly regardless of
food products are being processed under hygienic who does the cleaning. SSOPs should specify the
conditions. Cleaning and sanitation is best done following:
by a specially trained sanitation and cleaning crew • What: identifies task
and not by production personnel. A sanitation • Why: purpose of task
program in a food processing plant consists of two • When: frequency of task
main elements: a master sanitation schedule and a • Who: person responsible for task
monitoring program. • How: steps for completing task
Master Sanitation Schedule. A written master A sample SSOP for sanitation of drains might take
sanitation schedule should be in place to ensure the following form:
that all areas of a food processing plant or packing
shed are cleaned and sanitized on a regular basis.
The master sanitation schedule should detail the
area to be cleaned, the sanitation method, tools,
cleaning materials, and frequency of cleaning.
There are five steps involved in cleaning and
sanitizing:
1. Physical debris removal
2. Rinsing
3. Washing with detergent
4. Second rinsing
5. Sanitizing
Frequency: Daily
159
Table 3. Comparison of common sanitizers
__________________________________________________________________________________
Chlorine Iodine QUATS Acid-anionic
surfactants
__________________________________________________________________________________
Effectiveness against:
Effects on property:
Corrosive Fairly Slightly No Slightly
Maximum level permitted by 200 µL L-1 25 µL L-1 200 µL L-1 200-400 µL L-1
FDA without rinse based on type
160
Table 3. Comparison of common sanitizers—Continued
__________________________________________________________________________________
Chlorine Iodine QUATS Acid-anionic
surfactants
__________________________________________________________________________________
Incompatible with— Phenols, amines, Starch, silver Anionic wetting Cationic
soft metals agents, soaps, surfactants,
wood, cloth, alkaline
cellulose, nylon cleaners
Effective at neutral pH Yes No Yes No
__________________________________________________________________________________
Source: Adapted from Katsuyama and Strachan (1980)
*Type A: alkyl dimethyl benzyl ammonium chloride.
†
Type B: methyl dodecyl benzyl trimethyl ammonium chloride.
Chlorine is by far the most commonly used the location is noted. The plates are then incubated
sanitizer at 100 to 200 µL L-1. It is important that in the laboratory; if microbes were present on the
water containing chlorine be free from organic sampled surfaces, they will grow on the agar. A
matter and have a pH between 6.0 and 7.0. If low bacteria count per square centimeter means
either of these conditions is not met, then the that the sanitation crew is doing a good job at
chlorine is ineffective. cleaning and sanitizing. If the number of microbes
dramatically increases, an evaluation of sanitation
Monitoring Program. Before processing or procedures is in order.
packing begins, sanitation crew performance
should be evaluated on a daily basis to ensure Surface Swabbing. A variation of the petri plate
that conditions are hygienic. Visual inspection method is to use sterile swabs to collect samples
should be performed to ensure that no food from food contact surfaces. Wet sterile swabs are
particles or foreign matter are present on used to brush an area of a food contact surface.
processing equipment. In particular, areas that The swab is then placed in a container with sterile
are difficult to clean should be inspected, such solution. Bacteria are counted after incubation
as the underside of conveyors and peeling as above. Swabs and films for environmental
equipment. Unfortunately, visual inspection is sampling are commercially available from several
not enough to ensure that equipment has been companies.
sanitized properly. The number of microbes
present on processing equipment after sanitation Bioluminescence. The contact petri plate or swab
operations should be determined on a regular basis methods are good for monitoring sanitation
to evaluate sanitation crew performance. Such crew performance, but results are not available
determination can be made using one of three immediately. Another microbe detection method,
methods: petri contact plate, surface swabbing, or called bioluminescence, is capable of detecting the
bioluminescence. presence of microbes immediately. This method
relies on measuring the amount of ATP (adenosine
Petri Contact Plate. Plastic petri plates or triphosphate) that is present on food contact
films contain sterile agar with growth media surfaces. ATP is present in all living cells and
for microbes and the type of microbes that will thus is a good indicator of the presence of organic
grow on these plates is determined by the type of material. This test is similar to the swab testing
medium used. In this method, petri plates or films method except that the cleanliness of equipment is
are pressed up against food contact surfaces and determined within minutes after the swab is taken.
161
In this test, equipment is swabbed and the amount of CCPs. Each HACCP program is unique and
of ATP present is determined by a chemical test must be tailored to the specific operation.
kit. These test kits are available from a number
of suppliers. Bioluminescence test results are There are seven basic steps in an HACCP
available immediately and can determine if program:
cleaning and sanitation procedures must be 1. Conduct a hazard analysis
repeated before processing or packing begins. 2. Determine CCPs to control the identified
hazards
3. Establish critical limits for each CCP
Hazard Analysis Critical Control Points 4. Establish CCP monitoring requirements
(HACCPs) 5. Establish corrective actions to be taken
when a CCP is outside critical limits
HACCP is a food safety system developed by 6. Establish record-keeping systems to
the Pillsbury Company to reduce risk associated document the HACCP program
with food eaten by astronauts during space flights. 7. Establish procedures to verify that the
HACCP is a system for the prevention of physical, HACCP program is functioning as intended.
chemical, or microbial contamination of food. The
prime function of HACCP is to prevent identified Assessment of Hazards
hazards in food preparation through control of the
process. HACCP functions as the final stage of Each unit operation should be evaluated to
an integrated food safety program and includes identify potential sources of microbial, chemical,
Good Agricultural Practices (GAPs), Good and physical hazards that may be introduced
Manufacturing Practices (GMPs), and Sanitation into produce. Areas that should be evaluated
Standard Operating Procedures (SSOPs). In fact, are growing and harvesting operations, packing
HACCP can only be effective if these programs shed operations, packaging material and storage
are in place and functioning properly. There is no areas, and all steps in distribution. This process is
minimum or maximum number of Critical Control best accomplished by a team consisting of both
Points (CCPs) in any given operation. What is management and production personnel. (Example:
important is that all potential hazards be addressed
Hydrocooling water contamination, microbial or
through prerequisite programs or HACCP. Those
chemical.)
hazards that can be controlled or minimized
through quantitative control of a process may
Determination of CCPs to Control the Identified
be designated CCPs and included in a HACCP
Hazards
program. Fresh-cut processors may have as few as
two Critical Control Points (CCPs) in a perfectly
The next step in developing a HACCP program is
adequate HACCP plan.
to draw a flow diagram for the specific operation
and then determine where each of the identified
HACCP is a systems approach to ensure safety
hazards may be prevented. Each point that will
of a food product; it is not a means of ensuring
be monitored to control a specific hazard may be
food quality. Prevention of physical, chemical,
designated a CCP. (Example: A chlorine injection
and microbial contamination of produce during
system on a hydrocooler.)
packing or processing is essential to ensure
production of a safe product. It is recommended
Establishment of CCP Limits
that each produce handling operation identify an
individual for formal HACCP training and to be in
Once CCPs have been identified, critical limits
charge of a team responsible for implementing the
must be set to determine when corrective actions
HACCP program. HACCP programs should be as
need to be taken. Limits must be observable and
simple as possible, without an excessive number
measurable. (Example: Hydrocooler water must
162
have a chlorine level of 100 to 150 µL L-1 total Application of HACCP
chlorine and a pH of 6.0 to 7.5.)
When considering applying these principles
Establishment of CCP Monitoring Procedures to a farm operation, one can immediately see
the difficulty in controlling naturally occurring
It is critical to define clearly how often monitoring hazards. For example, bird droppings in an
will be done, how measurements will be taken, orchard may potentially represent a hazard from
and what documentation will be prepared. the spread of E. coli O157:H7 or Salmonella spp.
(Example: Hydrocooler water pH and chlorine However, it may not be a CCP because there is
levels will be monitored hourly using a test kit and no way to prevent that hazard by controlling a
continuously with a strip chart recorder that has process. Furthermore, there is no way to quantify
been calibrated daily; hourly pH and chlorine level and measure bird droppings to know if they are
measurements will be recorded in writing; and the within critical limits. The same would also be true
records will be made available for inspection at of Clostridium botulinum spores in soil. Though
the hydrocooler.) they may represent a potential hazard, it would not
be appropriate to establish soil as a CCP because
Corrective Action When Deviations From it is not practical to measure the spores in soil or
Critical Limits Occur to control them through any known process. In
fact, most agricultural hazards cannot, and should
When a deviation from the prescribed limits not, be prevented through HACCP. Instead, the
occurs, corrective action must be taken to use of GAPs has been identified by the FDA and
eliminate potential contamination. All deviations the produce industry as a more appropriate way to
and corrective actions must be documented in address these hazards.
writing. (Example: Chlorine levels are determined
to be below 25 µL L-1. Hydrocooling of product Another example is a cold storage room in a
is stopped, chlorine levels are adjusted, and all packinghouse where condensed water from
products that had been hydrocooled since the last refrigeration coils may contain the bacterium
time the system was verified to be within critical Listeria monocytogenes and could drip on the
limits are disposed of.) product. This is certainly a significant hazard,
but is it a CCP? It would not be practical to
HACCP Recordkeeping Systems develop a process to prevent water from dripping
or to quantify and monitor water dripping from
All paperwork related to the HACCP system refrigeration coils. A more appropriate way to deal
must be kept in an orderly and accessible manner. with this hazard is through SSOPs. Refrigeration
Paperwork kept should include production records coils and drip pans should be cleaned and
(for example, supplier audits), harvesting records sanitized according to a predetermined schedule
(for example, harvest dates and lot numbers), CCP to prevent the growth of L. monocytogenes in the
monitoring records, and deviation file (HACCP condensate. This way, the hazard is prevented
deviations and corrective actions taken). more effectively and more simply than by
designating a CCP.
HACCP Verification
There is no minimum or maximum number of
Periodic HACCP plan review, including review CCPs in any given operation. What is important
of CCP records, deviations, and random sampling is that all potential hazards be addressed through
must be conducted to ensure that the HACCP prerequisite programs or through HACCP. Those
program is functioning properly. hazards that can be controlled or minimized
through quantitative control of a process may
163
be designated CCPs and included in a HACCP Emphasis should be placed on preventing
program. Fresh-cut processors may have as few as contamination by pathogens. The best way to
two CCPs in an adequate HACCP plan. prevent the introduction of pathogens into fresh-
cut produce is by employing GAPs, GMPs,
The Fresh-Cut Industry SSOPs, and, in some cases, by implementing
an effective HACCP program. Such a program
Consumers expect that fresh-cut processors will identifies potential points of contamination and
manufacture wholesome and nutritious foods. ensures that those potential hazards are controlled
To do this, fresh-cut processors must have and monitored to enhance safety. HACCP and
systems in place to ensure that products being food safety do not begin and end at the doors of
manufactured do not have physical, chemical, the handling facility. They require that the produce
or microbial contaminants introduced during handler work with both suppliers and customers
processing and packaging. If such systems to maintain food safety throughout the production,
are not in place, consumers are at risk and a distribution, and marketing chain.
single incidence of personal injury traced back
to a specific food manufacturer may put that
company out of business and result in criminal Sprouts—A Special Case
prosecution of the owners and management.
Ensuring that food products are manufactured Over the past several years, sprouts have become
in a safe and wholesome manner does add cost a common fresh produce item linked to foodborne
to the final product. However, the long-term illness. A scientific advisory group to the FDA has
success of every food processor depends on its recognized sprouts as a special problem. This is
ability to consistently produce safe products. because bacterial pathogens that may be present
Food safety should not be confused with food at very low levels on sprout seeds at the time of
quality. Food safety programs simply ensure that sprouting can multiply to very high levels during
food products are safe to consume and prevent the 3- to 5-day sprouting process.
injury to consumers. Food safety does not begin
at the processing plant receiving dock and the Most sprout outbreaks have been caused by seed
production of raw ingredients should be done that was contaminated with a bacterial pathogen
following GAPs. before sprouting began. Pathogens can survive
for months under dry conditions used for seed
Fresh-cut produce can be damaged through storage. Though contaminated alfalfa seeds have
peeling, cutting, slicing, or shredding. These same been identified as the source in many outbreaks,
operations can transfer pathogenic microbes from clover, radish, and bean sprouts have also been
the surface of the intact produce to the internal associated with outbreaks. Any type of sprout seed
tissues. Injured cells and released cell fluids may potentially be contaminated with bacterial
provide a nourishing environment for microbial pathogens before sprouting.
growth. Maintaining low temperature throughout
distribution is critical to maintaining quality of The FDA published guidelines for sprout
fresh-cut fruits and vegetables. Low temperatures processors to reduce the potential for foodborne
reduce enzymatic reactions and greatly slow down illness related to sprouts. The guidelines include
the multiplication of spoilage organisms. Low treatment of seeds in a sanitizer solution (currently
temperatures also prevent the multiplication of a special allowance for 20,000 µL L-1 chlorine)
most foodborne pathogens, with the exception of prior to sprouting, as well as testing of the sprout
Listeria monocytogenes and a few others that are wash-water for Salmonella, E. coli O157:H7, and
capable of growing, albeit slowly, at refrigerated L. monocytogenes prior to harvest.
temperatures.
164
The FDA has published two related documents, Zhuang, R.Y., L.R. Beuchat, and F.J. Angulo.
entitled “Guidance for Industry: Reducing 1995. Fate of Salmonella montevideo on and in
Microbial Food Safety Hazards for Sprouted raw tomatoes as affected by temperature and
Seeds” and “Guidance for Industry: Sampling treatment with chlorine. Appl. Environ. Microbiol.
and Microbial Testing of Spent Irrigation Water 61: 2127-2131.
During Sprout Production.” These guidelines are
intended to provide recommendations to suppliers
of seed for sprouting and to sprout producers Further Reading
about how to reduce microbial food safety hazards
found in the production of raw sprouts. The Guidance for Industry: Reducing Microbial Food
guidelines are also intended to help ensure that Safety Hazards for Sprouted Seeds, October 27,
sprouts are not a cause of foodborne illness and 1999, Federal Register, vol. 64, no. 207. (U.S.
that those in the sprout industry comply with food Food and Drug Administration, Center for Food
safety provisions of the Federal Food, Drug, and Safety and Applied Nutrition, 200 C Street,
Cosmetic Act. Washington, DC.; http://www.gpo.gov/fdsys/pkg/
FR-1999-10-27/pdf/99-27976.pdf
Katsuyama, A.M., and J.P. Strachan. 1980. Beuchat, L.R. 1998. Surface Decontamination
Principles of Food Processing Sanitation. The of Fruits and Vegetables Eaten Raw: A Review.
Food Processors Institute, Washington, DC. World Health Organization, Geneva, Switzerland.
Suslow, T. 1999. Addressing animal manure Current Good Manufacturing Practices (GMPs)
management issues for fresh vegetable production. Regulations 21 CFR 100-169. Superintendent of
Perishables Handling 98:7-9. Documents, U.S. Government Printing Office, 732
North Capitol Street, NW, Washington, DC.
165
Nutritional Quality and Its Dietary supplements, while advantageous for
Importance to Human Health conditions in which specific nutrients are needed
in abundance such as iron deficiency, may be
Adel A. Kader, Penelope Perkins-Veazie, and poorly absorbed. Climatic conditions, particularly
Gene E. Lester temperature and light intensity, have an especially
strong effect on the nutritional quality of fruits
Kader was with the Department of Pomology, and vegetables (Mozafar 1994). Low temperatures
University of California, Davis, CA. He is favor synthesis of sugars and vitamin C (glucose
deceased. Perkins-Veazie is formerly with being the precursor to ascorbic acid) and at the
the South Central Agricultural Laboratory, same time decrease the rate of ascorbic acid
Agricultural Research Service, U.S. Department oxidation. Maximum β-carotene (vitamin A)
of Agriculture, Lane, OK. She is now with the content in tomatoes occurs at a temperature range
Department of Horticultural Science, North of 15 to 21 ºC (59 to 70 ºF), but β-carotene content
Carolina State University, North Carolina is reduced if temperatures are higher or lower
Research Campus, Kannapolis, NC. Lester is than this range, principally due to the temperature
formerly with the Kika de la Garza Subtropical sensitivity of lycopene, the precursor to β-carotene
Agricultural Research Center, Agricultural and lutein.
Research Service, U.S. Department of Agriculture,
Weslaco, TX. He is now with the Office of The B vitamins are crop specific when it comes
National Programs, Agricultural Research Service, to temperature sensitivity. Warm-season crops
U.S. Department of Agriculture, Beltsville, MD. (beans, tomatoes, peppers, melons, etc.) produce
more B vitamins at high (27 to 30 ºC, 81 to 86 ºF)
Fruits, nuts, and vegetables play a significant than at low (10 to 15 ºC, 59 to 70ºF) temperatures.
role in human nutrition, especially as sources Conversely, cool season crops (broccoli, cabbage,
of dietary fiber, minerals, and vitamins—C spinach, peas, etc.) produce more B vitamins at
(ascorbic acid), A, thiamine (B1), niacin (B3), low than at high temperatures. Light intensity
pyridoxine (B6), folacin (also known as folic has little effect on the B vitamins; but, as light
acid or folate), B9, and E (Quebedeaux and Bliss intensity increases, vitamin C increases and total
1988, Quebedeaux and Eisa 1990, Craig and Beck carotenoids (vitamin A precursors) and chlorophyll
1999, Wargovich 2000). Their contribution as a decrease (Gross 1991). Higher light intensities
group is estimated at 91% of vitamin C, 48% of produce more sugars, leading to more vitamin C,
vitamin A, 30% of folacin, 27% of vitamin B6, and also increase plant temperatures, inhibiting
17% of thiamine, and 15% of niacin in the U.S. β-carotene (vitamin A) production, which protects
diet. Fruits and vegetables also supply 16% of chlorophyll from photobleaching. Soil type, the
magnesium, 19% of iron, and 9% of calories. rootstock used for fruit trees, mulching, irrigation,
Legume vegetables, potatoes, and tree nuts, such fertilization, and other cultural practices influence
as almond, filbert, pecan, pistachio, and walnut, the water and nutrient supply to the plant, which
contribute about 5% of the per-capita availability can affect the composition and quality attributes
of proteins in the U.S. diet, and their proteins are (appearance, texture, taste, and aroma) of the
of high quality because they contain essential harvested plant parts (Goldman et al. 1999).
amino acids. Nuts are a good source of essential Other environmental factors that affect fruit and
fatty acids, fiber, vitamin E, and minerals. vegetable nutritional quality are altitude, soil pH
Other important nutrients supplied by fruits and and salinity, production practices (organic versus
vegetables include riboflavin (B2), zinc, calcium, conventional and greenhouse versus field), ozone,
potassium, and phosphorus. Fruits and vegetables insect injury, and plant diseases.
remain an important source of nutrients in many
parts of the world and offer advantages over Maturity at harvest, fruit size, and harvesting
dietary supplements because of low cost and wide method influence the commodity’s quality and
availability. extent of physical injuries. Delays between
166
harvest and consumption or processing can dietary supplements in increasing LDL oxidation
result in losses of flavor and nutritional quality. resistance, lowering DNA damage, and inducing
The magnitude of these losses increases with higher repair activity in human volunteers who
exposure to temperature, relative humidity, and/ participated in a study conducted in France, Italy,
or concentrations of O2, CO2, and C2H4 outside The Netherlands, and Spain (Southon 2000).
the ranges that are optimum for each commodity In another study, adding antioxidant (vitamins
during the entire postharvest handling system (Lee A, C, and E) dietary supplements into the diet
and Kader 2000). Furthermore, processing and of cancer treatment patients who were eating a
cooking methods can greatly affect the nutritional balanced diet of fruits and vegetables negatively
value of fruits and vegetables. For instance, water- affected their radiotherapy and chemotherapy
soluble vitamins such as vitamin C and folate (Seifried et al. 2003). High consumption of
are lost when cooking water is discarded, while tomatoes and tomato products has been linked
fat-soluble compounds such as lycopene may be to reduced carcinogenesis, particularly prostate
stabilized or enhanced by cooking. cancer, and has been thought to be due to the
presence of lycopene, which gives red tomatoes
Fruits, nuts, and vegetables in the daily diet have their color (Giovannucci 2002). However,
been strongly associated with reduced risk for use of tomato powder effectively reduced
some forms of cancer, heart disease, stroke, and prostate carcinogenesis in rats, while lycopene
other chronic diseases (Quebedeaux and Bliss supplements, considered the primary active
1988, Quebedeaux and Eisa 1990, Produce for ingredient of tomatoes, had no effect (Boileau et
Better Health Foundation 1999, Prior and Cao al. 2003). Similar comparative studies are needed
2000, Southon 2000, Wargovich 2000, Tomas- on other constituents of fruits and vegetables
Barberan and Espin 2001, Hyson 2002, Goldberg and on the bioavailability of nutrients taken as
2003). Some components of fruits and vegetables dietary supplements or as foods that contain these
(phytochemicals) are strong antioxidants and nutrients.
function to modify the metabolic activation and
detoxification and disposition of carcinogens or Examples of the phytochemicals in fruits and
even influence processes that alter the course vegetables that have established or proposed
of the tumor cell (Wargovich 2000). Though positive effects on human health and their
antioxidant capacity varies greatly among fruits important sources are shown in tables 1 and
and vegetables (Prior and Cao 2000, Perkins- 2. Some changes in these tables are likely as
Veazie and Collins 2001, Kalt 2002), it is better the results of additional studies on effects of
to consume a variety of commodities rather than phytochemicals and their bioavailability on human
limiting consumption to a few with the highest health become available in the next few years.
antioxidant capacity. The Dietary Guidelines Meanwhile it is important to evaluate the validity
for Americans, 2010 (USDA and HHS 2010) in and dependability of the results of every study
part advises filling half your plate with fruits, before reaching conclusions for the benefit of
vegetables, cooked beans, and grains. Such a consumers.
healthy eating pattern also embodies food safety
principles to avoid foodborne illness. In some
countries, consumers are encouraged to eat up to
10 servings of fruits and vegetables per day.
167
Table 1. Nutritive constituents of fruits and vegetables that have positive effects
on human health and their sources
_____________________________________________________________________________
Constituent Sources Established or
proposed effects on
human wellness
_____________________________________________________________________________
168
Table 1. Nutritive constituents of fruits and vegetables that have positive effects
on human health and their sources—Continued
_____________________________________________________________________________
Constituent Sources Established or
proposed effects on
human wellness
_____________________________________________________________________________
Magnesium Spinach, lentils, okra, potato, banana, nuts, corn, Helps prevent
cashews osteoporosis; aids
nervous system,
teeth, and immune
system
Potassium Baked potato or sweet potato, banana and Helps prevent
plantain, cooked dry beans, cooked greens, hypertension (high
dried fruits (such as apricots and prunes), blood pressure),
winter (orange) squash, cantaloupe stroke, and
arteriosclerosis
--------------------------------------------------------------------------------------------------------------------
169
Table 2. Nonnutritive plant constituents that may be beneficial to human health
______________________________________________________________________________
Constituent Compound Sources Established or
proposed effects on
human wellness
_______________________________________________________________________________
Phenolic compounds
Proanthocyanins Tannins Apple, grape, cranberry, Help prevent cancer
pomegranate
Anthocyanidins Cyanidin, malvidin, Red, blue, and purple Help prevent
delphinidin, fruits (such as apple, heart disease,
pelargonidin, blackberry, blueberry, cancer initiation,
peonidin, cranberry, grape, diabetes,
petunidin nectarine, peach, plum cataracts, and
& prune, pomegranate, allergies; help
raspberry, and lower blood
strawberry) pressure
Flavan-3-ols Epicatechin, Apples, apricots, Help prevent
blackberries, plums, platelet
raspberries, strawberries aggregation and
cancer
Flavanones Hesperetin, Citrus (oranges, grapefruit, Help prevent cancer
naringenin, lemons, limes,
eriodictyol tangerines, etc.)
Flavones Luteolin, apigenin Celeriac, celery, peppers, Help prevent
rutabaga, spinach, cancer, allergies,
parsley, artichoke, guava, and heart disease
pepper
Flavonols Quercetin, Onions, snap beans, Help prevent heart
kaempferol, broccoli, cranberry, kale, disease and
myricetin, rutin peppers, lettuce cancer initiation;
are capillary
protectants
Phenolic acids Caffeic acid, Blackberry, raspberry, Help prevent
chlorogenic acid, strawberry, apple, peach, cancer; help
coumaric acid, plum, cherry lower cholesterol
ellagic acid
Carotenoids
170
Table 2. Nonnutritive plant constituents that may be beneficial to human health—
Continued
______________________________________________________________________________
Constituent Compound Sources Established or
proposed effects on
human wellness
______________________________________________________________________________
Lycopene Tomato, watermelon, Help prevent
papaya, Brazilian guava, cancer, heart
autumn-olive, red disease, and male
grapefruit infertility
α-Carotene Sweet potatoes, apricots, Help prevent/slow
pumpkins, cantaloupes, tumor growth
green beans, lima beans,
broccoli, brussel sprouts,
cabbage, kale, kiwifruit,
lettuce, peas, spinach,
prunes, peaches, mango,
papaya, squash, carrots
β-Carotene Cantaloupes, carrots, Help prevent cancer
apricots, broccoli, leafy
greens (lettuce, swiss
chard, etc.), mango,
persimmon, red pepper,
spinach, sweet potato
Xanthophylls Lutein, zeaxanthin, Sweet corn, spinach, Help prevent
β-cryptoxanthin corn, okra, cantaloupe, macular
summer squash, turnip degeneration
greens
Monoterpenes Limonene Citrus (grapefruit, Help prevent cancer
tangerine, etc.)
_______________________________________________________________________________
171
References Perkins-Veazie, P., and J.K. Collins. 2001.
Contribution of nonvolatile phytochemicals to
Boileau, T.W., Z. Liao, S. Kim, et al. 2003. nutrition and flavor. HortTechnology 11:539-546.
Prostate carcinogenesis in N-methyl-N-nitrosourea
(NMU)-testosterone-treated rats fed tomato Prior, R.L., and G. Cao. 2000. Antioxidant
powder, lycopene, or energy-restricted diets. J. phytochemicals in fruits and vegetables: diet and
Natl. Cancer Inst. 95:1578-86. health implications. HortScience 35:588-592.
Craig, W., and L. Beck. 1999. Phytochemicals: Produce for Better Health Foundation. 1999.
health protective effects. Can. J. Diet. Pract. Res. Dietary Guidelines: The Case for Fruits and
60:78-84. Vegetables First. The Foundation, Wilmington,
DE.
Giovannucci, E. 2002. A review of epidemiologic
studies of tomatoes, lycopene, and prostate cancer. Quebedeaux, B., and F.A. Bliss, eds., 1988.
Exp. Biol. Med. (Maywood) 227:852-9. Horticulture and Human Health. Contributions
of Fruits and Vegetables, Proceedings of the 1st
Goldberg, G., ed. 2003. Plants: Diet and Health. International Symposium on Horticulture and
The Report of a British Nutrition Foundation Human Health, Arlington VA, April 12-15, 1987,
Task Force. Blackwell Science, Oxford, United pp. 243. Prentice Hall, Englewood Cliffs, NJ.
Kingdom.
Quebedeaux, B., and H.M. Eisa. 1990.
Goldman, I.L., A.A. Kader, and C. Heintz. 1999. [Horticulture and Human Health: Contributions of
Influence of production, handling, and storage Fruits and Vegetables, Proc. 2nd Intl. Symp. Hort.
on phytonutrient content of foods. Nutr. Rev. and Human Health.] HortScience 25:1473-1532.
57:S46-S52.
Seifried, H.E., S.S. McDonald, D.E. Anderson, et
Gross, J. 1991. Pigments in Vegetables: al. 2003. The antioxidant conundrum in cancer.
Chlorophylls and Carotenoids. AVI Book, Van Cancer Res. 63:4295-4298.
Nostrand Reinold, New York, NY.
Southon, S. 2000. Increased fruit and vegetable
Hyson, D. 2002. The Health Benefits of Fruits consumption within the EU: potential health
and Vegetables: A Scientific Overview for benefits. Food Res. Intl. 33:211-217.
Health Professionals. Produce for Better Health
Foundation, Wilmington, DE Tomas-Barberan, F.A., and J.C. Espin. 2001.
Phenolic compounds and related enzymes as
Kalt, W. 2002. Health functional phytochemicals determinants of quality in fruits and vegetables. J.
of fruits. Hort. Rev. 27:269-315. Sci. Food Agric. 81:853-876.
Lee, S.K. and A.A. Kader. 2000. Preharvest and USDA and HHS. 2010. U.S. Department of
postharvest factors influencing vitamin C content Agriculture and U.S. Department of Health
of horticultural crops. Postharv. Biol. Technol. and Human Services. Dietary Guidelines for
20:207-220. Americans, 2010. 7th Edition, U.S. Government
Printing Office, December 2010, Washington, DC.
Mozafar, A. 1994. Plant Vitamins: Agronomic,
Physiological and Nutritional Aspects. CRC Press, Wargovich, M.J. 2000. Anticancer properties of
Boca Raton, FL. fruits and vegetables. HortScience 35:573-575.
172
Annual Culinary Herbs Precooling Conditions
173
is susceptible to chilling injury if stored below cut ends of stems. Low temperatures should be
12 °C (54 °F). The primary symptom of chilling maintained throughout the cold chain to minimize
injury is browning or blackening of the leaves pathological disorders and prolong shelf-life.
(Cantwell and Reid 1993). Chilling increases the susceptibility of basil to
decay.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Aharoni, N., O. Dvir, D. Chalupowisz, and
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and Z. Aharon. 1993. Coping with postharvest
1.8 at 20 °C (68 °F). To calculate heat production, physiology of fresh culinary herbs. Acta Hort.
multiply mg kg-1 h-1 by 220 to get BTU per ton per 344:69-78.
day or by 61 to get kcal per tonne per day.
Aharoni, N., A. Reuveni, and O. Dvir. 1989.
Modified atmospheres in film packages delay
Physiological Disorders senescence and decay of fresh herbs. Acta Hort.
258:255-262.
Yellowing and leaf abscission may occur due to
ethylene exposure, especially if herbs are held at Cantwell, M. 1997. Properties and recommended
10 °C (50 °F) or warmer. Basil is susceptible to conditions for storage of fresh fruits and
chilling injury if held below 12 °C (54 °F), the vegetables. At http://postharvest.ucdavis.edu.
main symptom being necrosis and browning or
blackening of the leaves. Cantwell, M.I., and M.S. Reid. 1993. Postharvest
physiology and handling of fresh culinary herbs. J.
Herbs, Spices, Medicinal Plants 1:83-127.
Postharvest Pathology
Gorini, F. 1981. Vegetable schedules. 2.
Molds and bacterial decay may develop, Leafy vegetables. Chervil. Informatore di
especially on mechanically damaged leaves or Ortoflorofrutticoltura 22:3-4.
174
Lange, D.D., and A.C. Cameron. 1994.
Postharvest shelf-life of sweet basil (Ocimum
basilicum). HortScience 29:102-103.
175
Apple Skin Color. Each variety has specific commercial
requirements for skin color ranging from green
Chris B. Watkins, Eugene Kupferman, and David or yellow for varieties such as ‘Golden Delicious’
A. Rosenberger and ‘Granny Smith’ to red for varieties such as
‘Red Delicious.’ Bicolored apples such as ‘Gala’
Watkins is with the Department of Horticulture, and ‘Braeburn’ are also popular. Some varieties
Cornell University, Ithaca, NY; Kupferman is with are currently marketable only if they meet strict
Washington State University, Fruit Research and standards for red color intensity and coverage.
Extension Center, Wenatchee, WA; Rosenberger There is a tendency for wholesalers to gradually
is with Hudson Valley Laboratory, Cornell increase color standards, thereby encouraging
University, Highland, NY. growers to select redder strains of previously
acceptable bicolored apples. Red color is not an
indicator of fruit maturity or quality, however.
Scientific Name and Introduction With few exceptions, the ground (background)
color requirement for apples is light green,
Malus × domestica Borkh., the apple, is a as yellowness is regarded as an indication
perennial of the Rosaceae family. The apple is of overmature or senescent fruit. Consumers
thought to have arisen in the Caucasus region generally prefer ‘Golden Delicious’ apples that
of southeastern Europe, and the tree is one of have a white skin color, rather than green or
the hardiest temperate zone species. The edible yellow. Consumers demand fully green ‘Granny
part of the fruit is composed of fleshy tissue that Smith’ apples without a red blush and 100% red
surrounds the five ovaries, as well as some ovary color for ‘Red Delicious.’
or carpel tissue. The parenchyma of the fused
bases of the calyx, corolla, and stamens constitutes Blemish. A high-quality apple in the marketplace
the major edible part of the fruit, though this is free from blemish, though there may be a
tissue is sometimes interpreted as being cortical. greater tolerance for defects in certain markets
The skin surrounding the fleshy parenchyma such as organic outlets. Occurrences of physically
tissue is made up of cuticle, epidermal, and induced damage such as bruising or stem-
hypodermal layers, with lenticels allowing gas punctures and physiological and pathological
diffusion across the skin. Cracks in the skin disorders are not acceptable in any market. The
surface are also important for gaseous exchange. prevalence of these defects can be affected greatly
The diffusion characteristics of the skin can affect by variety characteristics such as stem length, skin
the tolerance of different varieties to storage tenderness, softness of the fruit, and genetically
conditions. Examples include ‘Golden Delicious,’ based resistance to physiological and pathological
which tends to shrivel faster than other varieties disorders. The density of the flesh and the skin
because of breaks in the cuticle, and the Marshall thickness can also contribute to resistance of fruit
‘McIntosh’ strain, which is less tolerant to low O2 to bruising under normal handling conditions,
in CA storage than other strains because of higher and susceptibility to bruising can determine the
resistance of the skin to gas exchange. commercial success of a variety.
177
indices. Since a rise in ethylene production is programs in conjunction with their land grant
associated with initiation of ripening, it has universities. Currently in Washington, individual
been suggested that ethylene production or packinghouses conduct their own maturity
internal ethylene concentration (IEC) should be programs inline with their marketing strategies. In
a major determinant of harvest decisions (Lau each region, a wide range of maturity and quality
1985). However, relationships between ethylene indices are collected, and the optimum harvest
production and optimum harvest dates can be period (harvest window) is established for each
poor, and the timing, or presence, of increased variety (Anonymous 1986, Blanpied and Silsby
ethylene production is a function of cultivar. 1992, Beaudry et al. 1993. The strength of these
Factors such as a growing region, orchard within a programs lies not in reliance on absolute maturity
region, cultivar strain, growing season conditions, indices, but in discussion with industry personnel
and nutrition are greatly affected within a cultivar on changes in maturity and quality occurring over
(Watkins 2003). Therefore, ethylene production the harvest period. In this way, full participation
may not be relevant for determining harvest of of extension personnel, growers, and storage
some cultivars. operators can ensure that fruit of appropriate
storage potential are directed towards short-,
For some bicolored apples, background color is medium- or long-term storage.
considered an important harvest index. In some
cases, State regulations have been established to
set minimal harvest maturities; for example, in Grades, Sizes, and Packaging
California the starch index for ‘Granny Smith.’
While ethylene production is regarded as the only Grade Standards. U.S. grades are U.S. Extra
physiological indicator of apple fruit maturity, Fancy, U.S. Fancy, and U.S. No. 1, based
fruit of some varieties and growing regions are primarily on color requirements, but also on
often harvested well before autocatalytic ethylene freedom from decay, disorders, and blemishes,
production occurs, and therefore this measurement as well as firmness of fruit (Childers et al. 1995).
is commercially irrelevant. However, in some These Federal guidelines have been adopted
regions ethylene is used to determine when fruit by many States, but States may have additional
are too mature to be candidates for long-term grading and branding laws. Information pertaining
storage. It is generally recognized that no single to any State can be obtained from the local State
maturity index is appropriate across all varieties; Department of Agriculture. Washington State
growers have learned to rely on a combination of packers follow the grade standards of either U.S.
indices. Federal regulations or special Washington State
Grade Standards promulgated by the Washington
Other indices, such as flesh firmness and soluble State Department of Agriculture in conjunction
sugar content, are quality indicators rather than with USDA.
maturity indicators, as they are influenced greatly
by orchard factors; for example, fruit exposure to Cartons. Sizing is usually carried out by weight
light. During the preharvest season, firmness falls or fruit diameter but is independent of grade.
and sugar content continues to increase. However, Requirements for fruit size vary greatly by market,
these quality indicators provide information but in general, larger sizes bring greater returns.
that can be important to fruit performance in Most fruit are packed into bushel cartons, usually
storage. Both indices are increasingly used in the 40 lb (18.2 kg), depending on variety, and sold
marketplace as quality criteria by wholesalers, by count (fruit per carton). Apples are most often
especially in Europe. packed on four or five soft fiberboard trays made
from recycled newspaper. In some cases, the tray
The three major apple-growing regions in the is made of soft polystyrene. Cartons are often
U.S. are the States of Washington, New York, unvented. However, unvented cartons on pallet
and Michigan. They operate apple maturity stacks will cool slowly, detrimentally affecting
178
product longevity. Venting to improve cooling Table 1. Effect of cooling rate on firmness
rates of fruit is becoming more common. of rapid CA Empire apples
________________________________________
A two-layer carton that is wider, known as the Days to cool Days from Flesh firmness (N)
60×40 pack, is becoming more common in the to 0 °C harvest to after removal
United States and for export to Europe. It has the 3% O2 from CA
advantage of minimizing fruit handling as the ________________________________________
cartons are placed directly onto display racks at 1 4 63
retail. 7 4 58
14 4 52
Most apples are sold loose, though fruit are ________________________________________
increasingly available in polyethylene bags of Modified from Blanpied (1986).
3, 5, or 10 lb (1.4, 2.3, or 4.5 kg). These bags
were originally used for marketing smaller fruit Apple fruit can be cooled by room cooling,
but are now used for all qualities and sizes. Bags forced-air cooling, or hydrocooling. Forced-air
are most often sold in warehouse-type retail cooling and hydrocooling systems can be used to
stores. Consumer packages in which two to six rapidly reduce fruit temperatures, but they are not
apples, or a combination of fruits, are shrink- widely used for apples in the United States. Room
wrapped are becoming more popular in some cooling, in which normal air flow within the storage
retail outlets. Shrink-wrapped packages reduce the room cools the fruit, is the predominant method
time consumers spend in the produce section and in most regions. However, air flows around rather
also reduce loss caused by consumer sorting and than through bins of fruit, so this method is slow
handling of individual fruit. and inefficient. Rapid cooling is often difficult
to accomplish when rooms are filled rapidly and
refrigeration capacity was not designed for a large
Cooling Conditions fruit load. This problem can be overcome in two
ways. First, fruit can be separated and loaded into
The rate of cooling of apple fruit affects retention a number of rooms for precooling before being
of quality, but its importance varies according moved into long-term storage. A second option is
to variety, harvest maturity, nutritional status of to load only the quantity that can be handled by the
the fruit, and storage history. It is very important existing refrigeration system.
to rapidly cool apple varieties that mature in the
early part of the harvest season (summer varieties) When refrigeration capacity is a limiting factor,
since they will soften more rapidly than those that no more than two stacks of bins should be placed
mature in the later part of the harvest season. across the width of the storage room each day,
and that should be reduced to one stack if the air
Within a variety, apples tend to soften more temperature in the room is not down to 0 °C (32
rapidly at later stages of maturity than at earlier °F) by the next morning (Bartsch and Blanpied
stages. Effects of slow cooling are magnified as 1990). Faster cooling will be obtained if bins
storage length increases. Therefore, inadequate are placed in the downstream discharge of the
investment of resources at harvest to ensure rapid evaporator with pallet runners oriented in the
fruit cooling may not be apparent until late in the same direction as the air flow. Additional bins of
storage period when fruit may not meet minimum fruit should be stacked, no more than two high,
firmness standards for marketing. For example, in unfilled refrigeration rooms to cool overnight
a 1-day delay at 21 °C (70 °F) before cooling before loading into the CA room the next
results in a 7- to 10-day loss of storage life for morning. These stacks should be placed randomly
‘McIntosh.’ The effects of delays before cooling throughout the unfilled room to maximize air
of fruit, irrespective of timing of CA conditions, exchange with the fruit. Capacity to cool fruit
are illustrated for ‘Empire’ apples in table 1. depends on refrigeration capacity and room
179
design. A qualified refrigeration engineer should
assist in the development of a cooling program.
180
Table 2. Storage characteristics of several apple varieties
______________________________________________________________________________
Variety Potential months Superficial Comments
of storage scald
______________ susceptibility
0 ºC CA*
Air
______________________________________________________________________________
181
Table 2. Storage characteristics of several apple varieties—Continued
______________________________________________________________________________
Variety Potential months Superficial Comments
of storage scald
______________ susceptibility
0 ºC CA*
Air
______________________________________________________________________________
Mutsu 3-4 6-8 Slight Green apples have low eating quality.
Spartan 3-4 6-8 Slight Can be susceptible to high CO2; susceptible
to skin shrivel at 36 to 38 °F.
Stayman 2-3 5-7 High Will tolerate CO2 up to 5%, but usually
stored in 2 to 3% CO2; scald inhibitor
essential; susceptible to skin shrivel.
______________________________________________________________________________
* The potential months storage are for rapid CA and range from those obtained with standard CA
to those obtained with low-O2 and low-ethylene CA. Growing region affects storage periods
obtained even under optimal CA conditions.
Temperatures for air-stored fruit are affected Temperatures in storage rooms should be monitored
by sensitivity of the variety to low-temperature throughout the storage period using thermocouples
disorders. While lower temperatures usually result throughout the room (Bartsch and Blanpied 1990).
in firmer and greener fruit, some varieties such as It is dangerous to rely on a single thermometer
‘McIntosh’ can develop core browning, soft scald, at the door, as temperatures within stacks and
and internal browning when held at temperatures throughout the room may be lower or higher than
below 3 °C (37 °F). However, these disorders indicated by such readings. Faster fruit ripening
typically develop only in fruit kept for more than and greater refrigeration usage result when fruit
several months, so risks of low-temperature injury temperatures are too high (table 3). Excessive
are low for fruit kept in short-term storage (2 to 3 temperatures after packing due to lack of cooling
mo). An additional factor to consider in selecting or developing during transport to market can
storage temperatures is the effect of temperature reduce quality at the consumer level. Fruit
on RH requirements. It is easier to maintain RH temperatures can increase during packing; failure
above 90% at 1 °C (34 °F) than at 0 °C (32 °F). to remove heat may result in subsequent loss
Final decisions should be based on experience of firmness during transport (Kupferman 1994,
with the apple variety and advice of extension Watkins 1999).
personnel.
Cultivar Temperature
__________________________________________
-1 °C 0 °C 2.2 °C 3.3 °C 4.4 °C
__________________________________________________________________________
Mean 680 750 900 1,000 1,100
__________________________________________________________________________
* To convert BTU ton-1 day-1 to kJ ton-1 day-1, multiply by 1.055.
183
Controlled Atmosphere (CA) defrosting. When CA rooms are designed, the
Considerations refrigeration engineer should demand the largest
coil size feasible for the room. Operators have
Apples are the predominant horticultural been reducing the number of defrost cycles to an
commodity stored under CA conditions, but absolute minimum to optimize RH in the room.
the gas composition and storage temperature Some operators reduce the O2 to the minimum
conditions are specific to variety, growing safe level and then raise the temperature to 1 to 2
region, and sophistication of the equipment °C (34 to 36 °F) to minimize the need to defrost.
available for monitoring and controlling the Some storage rooms are outfitted with high-
atmospheres. Interactions occur between O2, pressure water vapor systems that add moisture to
CO2, and temperature. For example, low storage the room and are suited for operation at around 0
temperatures increase fruit susceptibility to low-O2 °C (32 °F). The air distribution system should be
injury. Also when very low O2 levels are used, designed to prevent condensation of water droplets
levels of CO2 should be reduced to prevent CO2 on fruit to prevent decay. The use of plastic, rather
damage. than wooden bins or poly tubes (bin liners), inside
wooden bins has also helped minimize shrivel of
The wide range of recommended atmospheres has ‘Golden Delicious.’
been reported by Kupferman (1997) and reflects
the above factors as well as strategies employed Once fruit have been cooled and CA conditions
by different industries. Information about CA established, CA storage regimes fall into one of
recommendations for varieties in any growing three categories, depending on level of equipment
region should be obtained from local extension and technology involved.
personnel.
Standard CA involves conservative atmosphere
Until the mid 1970s, 8 to 10 days was often conditions used with minimum risk of gas-related
required to load a CA room and an additional injuries (table 4). Control of these atmospheres
15 to 20 days was needed for fruit respiration to may be manual by daily reading and adjustment
lower O2 to 2.5 to 3%. Fruit quality resulting from or via computer-controlled equipment. The margin
these conditions gradually became unacceptable of safety is large enough so that fluctuations in
in the marketplace. Rapid CA is now standard gas concentrations in manually adjusted storages
practice in many apple industries. Nitrogen flushing should not cause fruit injury.
equipment enables O2 in CA rooms to be reduced
to less than 5% within a day or two of harvest,
though 4 to 7 days from the harvest of the first
fruit and placing into the storage room with CA
conditions is considered “rapid CA.” For certain
varieties, fruit core temperatures must be reduced to
predetermined thresholds before application of CA.
Even when rooms are filled over extended periods,
O2 concentrations are usually lowered by flushing
with N2, and it is becoming more common to use N2
flushing for resealing rooms that are opened briefly
to remove some of the fruit required for marketing.
Nitrogen used for flushing is either purchased in
tanks or generated on site.
184
Table 4. Atmospheric and temperature requirements for standard CA storage
of apples
__________________________________________________________________________
Variety CO2 O2 Temperature Low-O2*
__________________________________________________________________________
% % °C
185
Table 4. Atmospheric and temperature requirements for standard CA storage
of apples—Continued
__________________________________________________________________________
Variety CO2 O2 Temperature Low-O2*
________________________________________________________________________
% % °C
Mutsu 2-3 2 0 yes
Spartan 2-3 2-3 0 yes
Stayman 2-5 2-3 0 yes
__________________________________________________________________________
Modified from Kupferman (1997).
* Storage potential at 1.5 to 1.8% O2 (Eastern USA)
†
CO2 sensitive: keep CO2 well below the O2 level. If not treated with DPA, use 1.5% to 2%
CO2 during the first 30 days.
Low-O2 CA storage requires that fruit be kept 2. Avoid apples from orchard blocks that average
at O2 below 2%, but above the concentration at fewer than five seeds per apple. Low seed count
which fermentation will occur. Nondescriptive can be a problem with some varieties.
terms such as “ultralow” are sometimes used but 3. Reduce core temperatures to 1 to 2 °C within
should be avoided in favor of describing specific 2 days after harvest for ‘McIntosh’ and
O2 percentages. The safe O2 concentration varies ‘Empire.’ (In Washington, cooling can take
by variety (table 4) and region. ‘Delicious’ longer as it is done under CA, with the
apples from British Columbia, Canada, for exceptions of ‘Fuji’ and ‘Braeburn’).
example, can be stored safely at 0.7% (Lau 1997) 4. Decrease O2 to under 5% within 7 days after
allowing control of superficial scald without harvest, except for ‘Fuji’ and ‘Braeburn.’
use of diphenylamine (DPA). Fruit of the same 5. Raise storage temperatures from 0 to 2 °C to
cultivar from other growing regions may show reduce risk.
injury when stored at these low O2 levels (Lau 6. Obtain automatic gas analysis/control
et al. 1998). Strains within a variety can also equipment to eliminate O2 fluctuations that may
vary in sensitivity (Lau 1997). An extreme case lead to low-O2 injury.
is the Marshall strain of ‘McIntosh,’ where O2 7. Avoid use of postharvest drench with DPA
below 4 to 4.5% is not safe, whereas 2 to 3% O2 where possible; its application has been
is acceptable for other ‘McIntosh’ strains (Park associated with low-O2 injury, for example, for
et al. 1993). In general, it is necessary to increase varieties with low-O2 scald risk.
storage temperature when low-O2 CA storage is
used. A number of guidelines have been developed Low-ethylene CA storage is appropriate because
for safe operation of long-term CA storage. apple fruit are climacteric, with autocatalytic
Using the following techniques, it has also been ethylene production often beginning close to
possible to minimize risk of low-O2 injury in the harvest. However, rates of ethylene production can
Northeastern United States: vary greatly among apple varieties. An important
physiological effect of CA storage is inhibition
1. Apply low-O2 CA storage only to apples of either ethylene production or its action, due to
harvested early in their harvest window. lowered O2 or increased CO2.
Overmature fruit can be damaged by low-O2
storage.
186
Low-ethylene CA storage has been evaluated as ‘Gala’ and ‘Golden Delicious’ are CO2-tolerant
a method for reducing superficial scald, as a safe varieties that also benefit from rapid reduction of
substitute for low-O2 CA storage, and for retarding atmosphere. In Washington, fruit with moderate
flesh softening and other forms of senescence pulp temperatures can be placed into a low-O2
(Blanpied 1990). Low-ethylene CA storage (below environment without danger of CO2 damage.
1 µL L-1) was used successfully in New York for Rapid CA is valuable because it helps retain fruit
storage of the naturally low-ethylene-producing firmness and acidity better than slowly established
‘Empire’ apple, but it has been replaced by low-O2 CA on these varieties. Washington-grown ‘Gala’
storage. In general, low-ethylene CA storage has not and ‘Golden Delicious’ can be stored as low as
proven successful for maintenance of fruit quality 1.0% O2 with CO2 levels up to 2.5% at 1 °C (34
if levels of ethylene gas within the fruit cannot be °F). If the temperature is lowered below this point,
controlled, and generally the return on investment O2 is raised. Regular storage is 0 °C (32 °F).
in this technology has been poor.
‘Fuji,’ ‘Braeburn,’ and ‘Granny Smith’ are in the
Other methods used in conjunction with CA CO2-intolerant category. Their cells are densely
storage to maintain quality of apple fruit include packed, and air exchange within the fruit is
treatments using short-term stress levels of low therefore reduced. In Washington, these apples
O2 or high CO2. In varieties including ‘Granny must have the flesh temperature close to the
Smith,’ ‘Delicious,’ and ‘Law Rome,’ O2 of 0.25 storage temperature before the O2 is reduced.
to 0.5% for up to 2 weeks has resulted in control These varieties have a tendency to develop
of superficial scald (Little et al. 1982, Wang and internal browning, a CO2 damage symptom that
Dilley 2000). High-CO2 (15 to 20%) treatments is associated with a natural predisposition of the
before application of CA storage were used for variety (and preharvest factors as well as storage
maintenance of firmness of ‘Golden Delicious’ regime). CO2 should remain well below the O2
apples in northwestern North America but generally level at all times, and temperatures should be
are no longer recommended because of fruit slightly elevated. For example, fruit stored at 1.5%
damage (Blanpied 1990). O2 are stored with CO2 below 0.5% at 1 °C (34
°F) if fruit are appropriately mature at harvest. It
Under commercial conditions, fruit from CA is not advisable to store waxed fruit in boxes with
rooms should be sampled at monthly intervals polyliners in CA, as this can hinder air exchange
to detect development of any storage problems within fruit.
and therefore reduce the chances of major fruit
losses. Sampling should be carried out by placing ‘Red Delicious’ is somewhat CO2-tolerant and
representative samples of fruit near a sampling is also tolerant of rapid CA. However, producers
port in the door of the CA room. Samples should have not seen the dramatic positive effects of
be kept in mesh bags rather than plastic bags to rapid CA on ‘Delicious’ that have been noted on
prevent false positive readings for scald. ‘Golden Delicious’ or ‘Gala.’ ‘Delicious’ fruit
soften more rapidly in a bin than on the tree, so
CA should not be delayed after harvest. Typical
CA and Apple Varieties regimes for CA of nonwatercored ‘Delicious’ are
1.5% O2 and up to 2.0% CO2 at 0 to 1 °C (32 to 34
The selection of CA atmospheres and temperature °F).
must take into consideration the variety and, in
some cases as mentioned above, the strain of State regulations on CA storage cover both the
a particular variety, in addition to where it was safe operation and the use of the legal definition
grown. Experience in Washington has shown that of “controlled atmosphere” for stored apples.
varieties can be divided into two types: those Regulations include the rate of establishment
tolerant of high CO2 and those that are not. of CA conditions, the maximum level of O2
187
permitted, and the length of time fruit are in Physiological Disorders
CA. Since regulations vary from State to State,
operators need to contact their State Department A wide variety of physiological disorders are
of Agriculture. found in apple fruit, but susceptibility varies
by variety, preharvest factors, and postharvest
Many precautions must be taken to assure the safe conditions (Lidster et al. 1990, Smock 1977).
operation of CA storage rooms. Operators must be Disorders can be considered in three categories:
aware of the risks of working with O2 levels below
those needed for survival. Additional precautions
must be taken when working with CA generators Disorders That Develop Only on the Tree. The
to avoid implosion or explosion hazards. In short, most important of these is watercore, in which
workers must be thoroughly trained. Contact your intercellular air spaces in the core and cortical
local extension office for information about safety tissues become filled with liquid, predominantly
in CA. sorbitol (Marlow and Loescher 1984). Usually
the occurrence of watercore is associated
with advancing fruit maturity and low night
1-Methylcyclopropene (1-MCP) temperatures prior to harvest, but a variant of
the disorder can occur as a result of heat stress.
Softening, yellowing, respiration, loss of titratable Presence of watercore in fruit at harvest creates
acidity, and sometimes a reduction in SSC, as well problems in certain varieties such as ‘Delicious’
as development of several physiological disorders, because fruit with moderate or severe watercore
are delayed or inhibited by 1-MCP application can develop breakdown during storage. By
(Watkins 2002, Watkins and Miller 2004). comparison, grade standards for ‘Fuji’ were
Responses of fruit to 1-MCP may be affected by modified so that watercore in ‘Fuji’ apples is not
cultivar and fruit maturity. Volatile production a grade defect in the United States or Canada,
by apples also is inhibited by 1-MCP, being because watercore is a desirable feature for this
consistent with the view that volatile production cultivar due to the sweetness it imparts to the
is regulated by ethylene. Consumer studies on fruit. Mild or moderate watercore should not be
acceptability of 1-MCP treated fruit are required a problem in storage of ‘Fuji’ if fruit are cooled
to ensure flavor is not unacceptably compromised. prior to reduction of O2. Severely watercored fruit
should not be placed in CA since breakdown will
develop over time.
Retail Outlet Display Considerations
Disorders That Develop on the Tree and During
Keeping apple fruit cold reduces metabolic rates Storage. Bitter pit is a disorder characterized by
and maintains fruit quality, but the trend in most development of discrete pitting of the cortical
new retail outlets is not to have refrigeration in flesh, the pits being brown and becoming
display tables. Moreover, typical displays in U.S. desiccated with time (Ferguson and Watkins
supermarkets employ stacking of loose fruit with 1989). The pits may occur predominantly near
increased risk of bruising. There is some movement the surface or deep in the cortical tissue. An
towards use of display cartons such as the 60×40 associated disorder, known as lenticel blotch, is
box, which reduces the need to handle fruit and also observed in some varieties. The incidence
ensures more rapid turnover of product. and severity of bitter pit are affected by variety,
but within a variety, bitter pit is related to
harvest date and climate. In susceptible varieties,
harvest of less mature fruit can result in higher
bitter pit incidence, as can excessive pruning or
high temperatures and/or droughty conditions
during the growing season. Effects of climatic
188
conditions are at least partly related to low to reduce decay incidence, and calcium salts may
calcium concentrations in the fruit. Development also be included at the same time to reduce bitter
of bitter pit during storage results in financial loss, pit or senescent breakdown. Application of label
and a number of strategies have been employed rates of clean DPA should prevent DPA-induced
to prevent its occurrence (Ferguson and Watkins fruit damage and exceed residue tolerances. The
1989). These include prediction of risk based on risk of DPA damage to fruit increases if DPA is not
mineral (mainly low calcium) content at harvest discarded when soil accumulates in the solution.
or infusion of magnesium. Rapid cooling, CA Both DPA use and DPA residues on fruit are
storage, and application of postharvest calcium prohibited in some countries. Another antioxidant,
drenches may be able to reduce its occurrence. ethoxyquin, is no longer permitted for use on
Recommended rates for application of calcium apples. Low levels of O2 in CA storage reduce
vary by variety and region; product labels should the risk of scald developing and also may permit
be followed in conjunction with advice of the use of lower DPA concentrations. Alternative
local extension specialist. Preharvest applications ways of controlling superficial scald are being
of calcium may be far more effective than investigated, and storage operators are reducing
postharvest drenching as a means of increasing the use of DPA where possible. Low-O2 and low-
concentration of fruit calcium and reducing bitter ethylene CA storage also reduce scald incidence.
pit. In British Columbia, Canada, 0.7% O2 storage is
used as a substitute for DPA treatment (Lau 1997).
Disorders That Develop During Storage. This technique cannot be used universally because
These can be divided into senescent breakdown fruit grown in other regions may be susceptible to
disorders, chilling disorders, and disorders low-O2 injury or the risk of scald may be greater
associated with inappropriate atmospheres during due to climate or variety.
storage. Senescent breakdown incidence is related
to harvest of overmature fruit or fruit with low Soft scald is characterized by irregular but
calcium content. It can be exacerbated by storing sharply defined areas of soft, light-brown tissue
fruit at higher than optimal temperatures. Fruit of that may extend into the cortex. Susceptibility of
susceptible varieties are commonly drenched with fruit to soft scald is variety- and climate-related,
calcium before storage, but incidence of senescent but effects of harvest maturity are inconclusive.
breakdown can also be reduced by harvesting fruit Overmaturity is almost always a contributing
at a less mature stage, rapid cooling, and reducing factor in ‘Golden Delicious.’ Storing fruit at 3 °C
storage duration. The most common disorders rather than at lower temperatures can sometimes
associated with temperature and atmospheres control the disorder, and DPA used for control of
are superficial scald, soft scald, low-temperature superficial scald may also reduce incidence of soft
breakdown, brown core, internal browning, scald. Storage at a lower temperature following
low-O2 injury, and high-CO2 injury. prompt cooling can reduce the incidence of soft
scald on ‘Golden Delicious.’
189
underneath the skin. In contrast to senescent cultivars of apples can also be invaded via the
breakdown, the affected tissues are more likely stem during long-term CA storage (Rosenberger
to be firmer, moister, and darker in color. Brown 1999).
core (coreflush) involves browning of the flesh,
initially in the core area and later in the cortex, Numerous other pathogens can also appear in
where it becomes difficult to distinguish from stored apples (Pierson et al. 1971, Snowdon
low-temperature breakdown. Internal browning 1990). Some postharvest pathogens infect fruit
does not involve breakdown of the flesh, but rather in the field but remain latent or quiescent until
a graying of flesh that becomes apparent when after apples are harvested and placed into storage.
apples are cut. Internal browning and coreflush are These include the Colletotrichum species that
often associated with higher CO2, since both can cause bitter rot; Botryosphaeria species that cause
occur in CA when CO2 is higher than O2. black rot and white rot; and Pezicula malicortis,
the cause of bull’s eye rot (Rosenberger 1990).
Low-O2 injury affects fruit in a number of ways. Postharvest decays initiated in the field must
The first indication of injury is loss of flavor, be controlled using fungicides or other disease
followed by fermentation-related odors. These management strategies during the growing season.
odors may disappear if storage problems are
identified soon enough and severe injury has not Blue mold and gray mold have been controlled
occurred. Injury symptoms range from purpling since the early 1970s by using postharvest
or browning of the skin in a red-colored variety, applications of benzimidazole fungicides.
to development of brown soft patches resembling Thiabendazole (TBZ), benomyl, and thiophanate-
soft scald, to abnormal softening and splitting of methyl were all registered for postharvest use on
fruit. As discussed earlier, varieties vary greatly apples until the postharvest labels for benomyl and
in response to low O2, and susceptibility to injury thiophanate-methyl were withdrawn in the early
is influenced by a number of preharvest and 1990s. TBZ is usually applied immediately after
postharvest factors. harvest in combination with the antioxidant DPA
(Hardenburg and Spalding 1972). TBZ may be
CO2 injury may be external or internal. The applied a second time as a line spray or in wax as
external form consists of wrinkled, depressed, apples are packed.
colorless or colored areas restricted to the skin
surface and usually on the greener side of the fruit. Benzimidazole-resistant strains of P. expansum
Internal forms are expressed as brown heart and/or and B. cinerea were discovered in apple
cavities in the flesh. DPA can reduce incidence of storage during the mid to late 1970s. However,
both external and internal CO2 injuries. postharvest application of a benzimidazole plus
DPA continued to control blue mold and gray
mold because most benzimidazole-resistant
Postharvest Pathology strains of the pathogen were highly sensitive to
DPA (Rosenberger and Meyer 1985, Sharom
The main postharvest diseases of apples that and Edgington 1985). During the mid 1990s, the
develop in storage are blue mold caused by incidence of blue mold began to increase in some
Penicillium species and gray mold caused by apple packinghouses where the predominant
Botrytis cinerea. Blue mold is the most common strains of P. expansum had developed resistance
and destructive of all the rots. Most blue mold to the benzimidazole-DPA combination. Gray
decays are caused by P. expansum, but P. solitum, mold is still controlled by the benzimidazole-
P. commune, and P. crustosum are also common DPA combination, presumably because it does
postharvest pathogens of apples (Sanderson and not recycle on field bins as readily as does P.
Spotts 1995). Penicillium species enter fruit expansum, and it therefore has been subjected to
primarily through cuts, stem punctures, and less selection pressure for fungicide resistance.
bruises (Wright and Smith 1954). However, some
190
Captan has a postharvest registration but has of biocontrols and reduced rates of TBZ may be
proven only moderately effective for controlling more effective than using either product alone
P. expansum and B. cinerea. Captan residues (Chand-Goyal and Spotts 1997). When such
are not acceptable in some export markets. combinations are used, the chemical fungicide
The fungicide fludioxonil is very effective for may provide eradicant and short-term protectant
controlling P. expansum on apples (Rosenberger et activity necessary to prevent decay until the
al. 2000). Fludioxonil is not yet approved for use biocontrol agents become established in wounds
on apples in the United States, but it may receive or other infection courts. Biocontrols provide
an EPA registration in the near future. some protection against TBZ-resistant strains
of the pathogens for which no other controls are
Much effort has been devoted to development currently available.
of biocontrols for postharvest diseases of apples
(Roberts 1990, Mercier and Wilson 1994, Regardless of the postharvest fungicide or
Wisniewski et al. 1995, Filonow et al. 1996, biocontrol strategies that may evolve from
Chand-Goyal and Spotts 1997, Janisiewicz current research, good sanitation will remain
1998). The controlled postharvest environment essential for reducing disease incidence. Inoculum
theoretically should allow selection of biocontrol for blue mold recycles from year to year on
agents particularly suited to those environments. contaminated field bins and in packinghouses
Many of the biocontrol agents selected and and cold storage rooms (Rosenberger 2001).
developed to date have proven very effective Recycling of P. expansum on field bins results in
in controlled tests, but commercialization of repeated exposure of the same pathogen strains
biocontrols has been slow. Product developers to postharvest fungicide treatments, thereby
view the market potential for postharvest contributing to more rapid selection of fungicide-
treatments as relatively limited because the resistant strains. Badly contaminated bins should
products are applied in a closed environment be cleaned and disinfested (steam-cleaned) before
rather than being sprayed over thousands of they are reused for a new crop. Plastic bins may
acres, as are conventional fungicides used to carry less inoculum than wooden bins. Plastic bins
protect crops in the field. Furthermore, liabilities also have the advantage of reducing bruising and
involved in postharvest treatment of apples are abrasion where apples contact the sides of bins.
considerable, both because of the value of the Careful fruit handling, rapid cooling after harvest,
stored crop and because apples as a commodity and storage at recommended temperatures also
have previously been spotlighted in debates help limit postharvest decays.
relating to food safety issues. In addition, devising
shelf-stable formulations of biocontrol agents
has been difficult. Some currently registered Quarantine Issues
products must be kept frozen until used. Activity
of biocontrol organisms may be compromised These vary widely according to marketplace and
by excessively high pathogen inoculum or by country and guidance should be sought from the
presence of other compounds in postharvest local Department of Agriculture.
treatment mixtures.
191
Additional Information Childers, N.F., J.R. Morris, and G.S. Sibbett.
1995. Modern Fruit Science - Orchard and
Many States have workshops and materials Small Fruit Culture, pp. 145-170. Horticulture
available. Washington, Michigan, and New York Publications, Gainesville, FL.
offer regular CA workshops, and proceedings are
available from Michigan State University and Ferguson, I.B., and C.B. Watkins. 1989. Bitter pit
Cornell University. Contact your local extension in apple fruit. Hort. Rev. 11:289-355.
office or horticulture society to obtain information
for your State. Filonow, A.B., H.S. Vishniac, J.A. Anderson,
and W.J. Janisiewicz. 1996. Biological control
of Botrytis cinerea in apple by yeasts from
References various habitats and their putative mechanisms of
antagonism. Biol. Contr. 7:212-220.
Anonymous. 1986. Apple Maturity Program
Handbook, 57 pp., Apple Maturity Program, Hardenburg, R.E., and D.H. Spalding. 1972.
Wenatchee, WA. Postharvest benomyl and thiabendazole
treatments, alone and with scald inhibitors, to
Bartsch, J.A., and G.D. Blanpied. 1990. control blue and gray mold in wounded apples. J.
Refrigeration and Controlled Atmosphere Storage Amer. Soc. Hort. Sci. 97:154-158.
for Horticultural Crops. Northeast Regional
Agricultural Engineering Service Bull. no. 22, 45 Hardenburg, R.E., A.E. Watada, and C.Y.
pp., (NRAES) PALS Publishing, Ithaca, NY. Wang. 1986. The Commercial Storage of Fruits,
Vegetables, and Florist and Nursery Stocks.
Beaudry, R., P. Schwallier, and M. Lennington. Agriculture Handbook 66. U.S. Department
1993. Apple maturity prediction: an extension of Agriculture, Agricultural Research Service,
tool to aid fruit storage decisions. HortTechnology Washington, DC.
3:233-239.
Ingle, M., and M.C. d’Souza. 1989. Physiology
Blanpied, G.D. 1986. Empire harvest dates and and control of superficial scald of apples. A
storage strategies to meet the market demand for review. HortScience 24:28-31.
fruit quality and condition. Proc. NY State Hort.
Soc. 131:150-166. Janisiewicz, W.J. 1998. Biocontrol of postharvest
diseases of temperate fruits: challenges and
Blanpied, G.D. 1990. Controlled atmosphere opportunities. In J. Boland and L. Kuykendall,
storage of apples and pears. In M. Calderon and eds., Plant-Microbe Interactions and Biological
R. Barkai-Golan, eds., Food Preservation by Control, pp. 171-198. Marcel Dekker, New York,
Modified Atmospheres, pp. 265-299. CRC Press, NY.
Boca Raton, FL.
Kader, A.A. Postharvest Technology of
Blanpied, G.D., and K.J. Silsby. 1992. Predicting Horticultural Crops, DANR Pub. no. 3311,
harvest date windows for apples. Cooperative University of California, Davis, CA.
Extension Information Bulletin 221, Cornell
University, Ithaca, NY. Kupferman, E. 1994. Report to the industry on
fruit quality and packing practices for Washington
Chand-Goyal, T., and R.A. Spotts. 1997. grown apples. Tree Fruit Postharv. J. 5:3-26.
Biological control of postharvest diseases of apple
and pear under semi-commercial and commercial
conditions using three saprophytic yeasts. Biol.
Control 10:199-206.
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Kupferman, E. 1997. Controlled atmosphere Park Y.M., G.D. Blanpied, Z. Jozwiak, and F.W.
storage of apples. In A.A. Kader, ed., 7th Liu. 1993. Postharvest studies of resistance to
International Controlled Atmosphere Research gas diffusion in McIntosh apples. Postharv. Biol.
Conference, University of California, Davis, Technol. 2:329-339.
July 13-18, 1997, vol. 2, pp. 1-31. University of
California, Davis, CA. Pierson, C.F., M.J. Ceponis, and L.P. McColloch.
1971. Market Diseases of Apples, Pears and
Lau, O.L. 1985. Harvest guide for B.C. apples. Quinces. Agriculture Handbook 376, U.S.
British Columbia Orchardist 7:1A-20A. Department of Agriculture, Agricultural Research
Service, Washington, DC.
Lau, O.L. 1997. The effectiveness of 0.7% O2 to
attenuate scald symptoms in ‘Delicious’ apples is Roberts, R.G. 1990. Postharvest biological control
influenced by harvest maturity and cultivar strain. of gray mold of apple by Cryptococcus laurentii.
J. Amer. Soc. Hort. Sci. 122:691-697. Phytopathology 80:526-530.
Lau, O.L., C.L. Barden, S.M. Blankenship, et al. Rosenberger, D.A. 1990. Postharvest diseases.
1998. A North American cooperative survey of In A.L. Jones and H.S. Aldwinckle, eds.,
‘Starkrimson Delicious’ apple responses to 0.7% Compendium of Apple and Pear Diseases, pp. 53-
O2 storage on superficial scald and other disorders. 54. APS Press, St. Paul, MN.
Postharvest Biol. Technol. 13:19-26.
Rosenberger, D.A. 1999. Postharvest decays:
Lee, C.Y., and N.L. Smith. 1995. Minimal research results and future directions. In CA
processing of New York apples. New York’s Food Storage: Meeting the Market Requirements,
and Life Sciences Bull. 145, Cornell University, pp. 49-62. NRAES Bull. 136, Northeast Regional
Ithaca, NY. Agricultural Engineering Service, Ithaca, NY.
Lidster, P.D., G.D. Blanpied, and R.K. Prange. Rosenberger, D.A. 2001. Postharvest decay
1990. Controlled-atmosphere disorders of control without fungicides. In Storage Workshop
commercial fruits and vegetables. Publication 2001: Apple Handling and Storage, pp. 21-
1847E, Agriculture Canada, Ottawa, Canada. 23. NRAES Bull. 153, Northeast Regional
Agricultural Engineering Service, Ithaca, NY.
Little, C.R., J.D. Faragher, and H.S. Taylor. 1982.
Effects of initial low-O2 stress treatments in low Rosenberger, D.A., and F.W. Meyer. 1985.
oxygen modified atmosphere storage of ‘Granny Negatively correlated cross-resistance to
Smith’ apples. J. Amer. Soc. Hort. Sci. 107:320- diphenylamine in benomyl-resistant Penicillium
323. expansum. Phytopathology 75:74-79.
Marlow, G.C., and W.H. Loescher. 1984. Rosenberger, D.A., C.A. Ahlers, and F.W. Meyer.
Watercore. Hort. Rev. 6:189-251. 2000. Evaluation of Scholar for controlling blue
mold of apples during storage, 1998-99. Fung.
Mercier, J., and C.L. Wilson. 1994. Colonization Nemat. Tests 55:24.
of apple wounds by naturally occurring microflora
and introduced Candida oleophila and their effect Sanderson, P.G., and R.A. Spotts. 1995.
on infection by Botrytis cinerea during storage. Postharvest decay of winter pear and apple fruit
Biol. Contr. 4:138-144. caused by species of Penicillium. Phytopathology
85:103-110.
193
Sharom, M.S., and L.V. Edgington. 1985. Watkins, C.B., and W.B. Miller. 2004. A summary
Temperature dependent negatively correlated of physiological processes or disorders in fruits,
cross-resistance between benomyl and vegetables and ornamental products that are
diphenylamine for Botrytis cinerea, Gerlachia delayed or decreased, increased, or unaffected by
nivalis, and Monilinia fructicola. Can. J. Plant application of 1-methylcyclopropane (1-MCP). At
Path. 7:389-394. http://hort.cals.cornell.edu.
Smock, R.M. 1977. Nomenclature for internal Wisniewski, M.W., S. Droby, E. Chalutz, and Y.
disorders of apples. HortScience 12:306-308. Eilam. 1995. Effect of Ca2+ and Mg2+ on Botrytis
cinerea and Penicillium expansum in vitro and on
Snowdon, A.L. 1990. A Color Atlas of Postharvest the biocontrol activity of Candida oleophila. Plant
Diseases and Disorders of Fruits and Vegetables, Pathol. 44:1016-1024.
vol. 1, General Introduction and Fruits. CRC
Press, Boca Raton, FL. Wright, T.R., and E. Smith. 1954. Relation of
bruising and other factors to blue mold decay
Thompson, J.F., F.G. Mitchell, T.R. Rumsey, R.F. of Delicious apples. USDA Circular 935, U.S.
Kasmire, and C.C. Crisosto. 1998. Commercial Department of Agriculture, Washington, DC.
cooling of fruits, vegetables, and flowers. DANR
Pub. no. 21567, University of California, Davis,
CA.
194
Apricot generally handled in half bins or totes and hand-
packed. In some cases, apricots are dry-dumped
Carlos H. Crisosto and Adel A. Kader onto a padded packing line belt. Apricots are
tray-packed in single and double layers or
Crisosto is with the Department of Plant Sciences, volume-filled (about 10 kg net). Apricots should
University of California, Davis, CA. Kader was be uniform in size, and not more than 5% in
with the Department of Plant Sciences, University each container may vary more than 6 mm when
of California, Davis, CA. He is deceased. measured at the widest part of the cross section.
Fruit size, shape, freedom from defects (including The major benefits of CA during storage and
gel breakdown and pit burn), and freedom from shipment are retaining fruit firmness and ground
decay are all important quality criteria. High color. CA conditions of 2 to 3% O2 + 2 to 3%
consumer acceptance is attained for fruit with CO2 are suggested for moderate commercial
high (>10%) SSC and moderate TA (0.7 to 1.0%). benefits; the extent of benefits depends on
Apricots with 2 to 3 lb-force (8.9 to 13.3 N) flesh cultivar. Exposure to less than 1% O2 may result
firmness are ready to eat. Most cultivars soften in development of off flavors, and higher than
very fast, making them susceptible to bruising and 5% CO2 for more than 2 weeks can cause flesh
subsequent decay. browning and loss of flavor. The addition of 5 to
10% CO2 as a fungistat during transport (less than
2 weeks) may improve the potential for benefit
Horticultural Maturity Indices from CA. Prestorage treatment with 20% CO2
for 2 days may reduce incidence of decay during
In California, harvest date is determined by subsequent transport and storage in CA or air.
changes in skin ground color from green to
yellow. The exact yellowish-green color depends
on cultivar and shipping distance. Apricots should Retail Outlet Display Considerations
be picked when still firm because of their high
bruising susceptibility when fully ripe and soft. Cold-table display is recommended because
of apricots’ fast ripening. Ripening before
consumption should ideally be done at
Grades, Sizes, and Packaging temperatures of 18 to 24 ºC.
195
Chilling Sensitivity Pit Burn. Flesh tissue around the stone softens
and turns brown when the apricots are exposed
Chilling-sensitive cultivars develop and express to temperatures above 38 °C (100 °F) before
chilling injury symptoms (gel breakdown, flesh harvest. This heat injury increases with higher
browning, and loss of flavor) more rapidly at 5 temperatures and longer durations of exposure.
°C than at 0 °C. Storage at 0 °C is necessary to
minimize incidence and severity of chilling injury
on susceptible cultivars. Postharvest Pathology
Respiration Rate
Quarantine Issues
-1 -1
Temperature mg CO2 kg h
0 °C 4 to 8 Apricots are currently exported from California
10 °C 12 to 20 to Canada and Mexico and imported from Chile
20 °C 30 to 50 and New Zealand. California apricot shipments
to Canada, except British Columbia, are not
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate restricted. For export to British Columbia and
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and Mexico, apricots must be free of oriental fruit
1.8 at 20 °C (68 °F). To calculate heat production, moth (Grapholita molesta/Cydia molesta). Apricot
multiply mg kg-1 h-1 by 220 to get BTU per ton per shipments require a phytosanitary certificate (PC)
day or by 61 to get kcal per tonne per day. and a clear statement that the fruit were produced
and inspected in accordance with the system
approach guidelines agreed to by APHIS and the
Physiological Disorders Canadian Food Inspection Agency.
Gel Breakdown or Chilling Injury. Develops For export to Mexico, a PC stating that the
in cold storage, particularly at 2 to 7 °C (35 fruit are free of western fruit tree leaf roller
to 45 °F) when apricots are stored for a long (Archips argyrospilus), navel orangeworm
time period. This physiological problem is (Amyelois transitella), oblique banded leaf
characterized in early stages by the formation of roller (Choristaneura rosaseana), orange tortrix
water-soaked areas that subsequently turn brown. (Argyrotaenia citrana), carob moth (Spectrobates
Breakdown of tissue is sometimes accompanied ceratoniae), omnivorous leaf roller (Platynota
by sponginess and gel formation. Fruit stored at stultana), and peach twig borer (Anarsia
these temperatures have short market-life and lose lineatella) is needed. Additionally, a clear
flavor. statement is required that the fruit were produced
196
and inspected in accordance with the Mexico work Chambroy, V., et al. 1991. Effects of different CO2
plan. A copy of the work plan for the exportation treatments on postharvest changes of apricot fruit.
of apricot fruit from the United States to Mexico Acta Hort. 293:675-684.
can be obtained from the PPQ regional office in
Sacramento, CA. The work plan is only valid for Claypool, L.L., and R.M. Pangborn. 1972.
the current season. Influence of controlled atmosphere storage on
quality of canned apricots. J. Amer. Soc. Hort. Sci.
Methyl bromide fumigation is required on apricots 97:636-638.
imported from Chile, but not from New Zealand.
In some cases, apricots may be precleared. To do Folchi, A., G.C. Pratella, S.P. Tian, and P.
this, the shipments must be accompanied by a Bertolini. 1995a. Effect of low oxygen stress in
PPQ Form 203 signed by the APHIS inspector on apricot at different temperatures. Ital. J. Food Sci.
site in Chile. 7:245-254.
198
Optimum Storage Conditions Respiration Rates
199
minor problem with fruit bruises and/or chemical- Couturier, G., E. Tanchiva, J. Gonzales, et al.
scalded areas caused by calcium dips of 4 to 8% 1996. Preliminary observations on the insect pests
(Hernández et al. 2003). of araza (Eugenia stipitata Mc Vaugh, Myrtaceae),
a new fruit crop in Amazonia. Fruits 51:229-239.
Fruit are highly susceptible to dehydration and Galvis, J.A., and M.S. Hernández. 1993b.
shriveling. A warming treatment of 18 h at 20 °C Comportamiento fisiológico del arazá (Eugenia
(68 ºF) with 90% RH after 6 days at 10 °C (50 stipitata) bajo diferentes temperaturas de
ºF) reduces skin scald and associated decay and almacenamiento [Physiological behavior of
extends shelf-life up to 2 weeks (Hernández et al. araza (Eugenia stipitata) under different storage
1999, 2002b). temperatures]. Colombia Amazónica 6:123-134.
200
Hernández, M.S., J. Barrera, J.P. Fernández- Saldanha, L.A., and N.M. Silva. 1999. Semi-
Trujillo, et al. 2002a. Efecto de la temperatura de artificial bearing of the larvae of Anastrepha
almacenamiento en la fisiología y calidad del fruto obliqua (Diptera:Tephritidae) in Manaus,
de arazá (Eugenia stipitata Mc Vaugh) [Effect of Amazonas-Brazil. Florida Entomologist 82:82-87.
storage temperature on postharvest physiology
and quality of araza fruit]. IV Iberian Congress. Swift, J.F., and W.E. Prentice. 1983. Native fruit
Spanish Soc. Hort. Sci., Cáceres, May 2001. Actas species of the ecuadorian Amazon: production
de Horticultura 37:1074-1081. techniques and processing requirements. Proc.
Amer. Soc. Hort. Sci. Trop. Reg. 27A:95-100.
Hernández, M.S., H.E. Arjona, O. Martínez,
and J.P. Fernández-Trujillo. 2002b. Influence of Tai Chun, P.A. 1995. Pre- and post-harvest pests
intermittent warming treatments on the postharvest and diseases of Araza (Eugenia stipitata) in Costa
quality of araza fruit (Eugenia stipitata McVaugh). Rica. Inter-American Institute for Cooperation on
In R. Dris and A. Sharma, eds., Food Technology Agriculture, Costa Rica.
and Quality Evaluation, vol. 43, pp. 261-265. Sci.
Pub. Inc., Plymouth, U.K.
201
Artichoke carton of about 10 kg (23 lb.). For example, size
18 buds means 18 buds per carton (also referred
Chien Yi Wang to as “18s”). Standard grades include 18s (>13
cm diameter, 5.4 in), 24s (10 to 13 cm, 4.0 to 4.5
Wang is formerly with the Food Quality in), 36s (8.5 to 10 cm, 3.5 to 4.0 in), 48s (7.5 to
Laboratory, Henry A. Wallace Beltsville 8.5 cm, 3.0 to 3.5 in) or 60s (6.5 to 7.5 cm, 2.75
Agricultural Research Center, Agricultural to 3 in) buds per box. Smaller buds (2.5 to 6.5
Research Service, U.S. Department of Agriculture, cm, 1.0 to 2.75 in) are often “jumble packed” at
Beltsville, MD. He is now retired. an average of 100 to 175 buds per box. The fresh-
produce market prefers 24s and 36s, but some
retailers prefer 36s and 48s, since artichokes are
Scientific Name and Introduction generally priced by bud, not by weight.
Cynara scolymus L., the globe artichoke, is a A new grade was adopted for U.S. No. 1 long
perennial of the Asteraceae (Compositae) family. stem globe artichokes in 2006. The stems must
The edible portion includes the tender immature be smoothly cut to a minimum of at least 20 cm (8
flower bud and fleshy central base that is protected in) unless otherwise specified to a longer length in
by a cone of short, thick-stemmed bracts. The connection with a grade.
main types include Green Globe, Desert Globe
Imperial Star, Emerald, and Big Heart. Artichokes
are primarily grown in California and are available Precooling Conditions
year round.
In order to maintain quality and storage life,
artichoke buds should be precooled to below 5 °C
Quality Characteristics and Criteria (41 °F) within 24 h of harvest (Lipton and Stewart
1963). Hydrocooling, forced‑air cooling, and
A high-quality artichoke will have tightly closed, package‑icing are common methods of postharvest
turgid outer bracts without signs of black tip, cooling of artichokes and will generally retard
blistering, or browning. It should be medium to deterioration such as discoloration, weight loss,
dark glossy green in color, and some cultivars may and decay.
have a magenta color at the base of each bract.
The artichoke should not be soft when squeezed or
feel heavy for its size. Both thorny and thornless Optimum Storage Conditions
cultivars are used commercially.
The recommended conditions for storage of
artichokes are 0 °C (32 °F) and >95% RH.
Horticultural Maturity Indices Artichoke buds can be kept in good condition for
2 weeks at 0 °C (32 °F), 10 days at 5 °C (41 °F),
The outer bracts of an artichoke ready for harvest and 5 days at 10 °C (50 °F) (Ryall and Lipton
should be tightly closed, firm, and turgid. They are 1979, Saltveit 1991).
harvested when immature and selected based on
size and compactness.
Controlled Atmosphere (CA)
Considerations
Grades, Sizes, and Packaging
A reduction in browning of the outer bracts is the
Grades include U.S. No. 1 and U.S. No. 2, based major benefit from CA storage when artichokes
primarily on external appearance. Buds are are stored at temperatures higher than 0 °C (32
classified by the number that fit into a standard °F). However, the effectiveness of CA storage
202
depends on bud maturity, cultivar, temperature, multiply mg kg-1 h-1 by 220 to get BTU per ton per
and the particular atmosphere used (Rappaport day or by 61 to get kcal per tonne per day.
and Watada 1958, Andre et al. 1980, Ryder et al.
1983). Optimal CA conditions vary widely among
cultivars, ranging between 1 to 6% O2 and 2 to Physiological Disorders
7% CO2 (Ryall and Lipton 1979, Saltveit 1997,
Andre et al. 1980, Escriche et al. 1982). Little Splitting of the bract tip is a common problem
or no beneficial effect on quality retention can caused by rough handling during and after harvest.
be obtained by CA storage when artichoke buds The surfaces of bracts are also easily bruised and
are stored at 0 °C (32 °F) (Miccolis and Saltveit scratched, so careful handling is important. The
1988). Therefore, no general recommendation abraded areas usually turn brown or black, which
can be made for CA storage. O2 below 2% may greatly detracts from appearance and quality and
result in internal blackening (Suslow and Cantwell provides a route through which microorganisms
1998). can enter. Also, violet discoloration of inner
bracts occurs, the severity of which was low when
artichokes were stored at temperatures below 10
Retail Outlet Display Considerations °C (50 °F) or above 25 °C (77 °F) (Bianco 1979)
and may have been due to low ethylene production
Use of both top ice and water sprinklers are (Ryder et al. 1983).
acceptable.
Postharvest Pathology
Chilling Sensitivity
The most common decay found in artichokes is
Artichokes are not sensitive to chilling and should
gray mold (Botrytis cinerea) (Moline and Lipton
be stored as cold as possible without freezing.
1987). The lesions most frequently begin on
wounds and spread to other areas of the bud.
Ethylene Production and Sensitivity Since storage at low temperatures slows the
rate of spread of the disease, fungal growth near
Artichokes produce only very low amounts of freezing temperature is minimal. Bacterial soft rot
ethylene and are not particularly sensitive to (Erwinia carotovora) may be a problem in storage
ethylene exposure. and distribution if optimum temperature is not
maintained. Therefore, low temperatures must be
maintained throughout the cold chain to minimize
Respiration Rates pathological disorders and prolong shelf-life.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate No current potential.
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
203
Special Considerations Miccolis, V., and M.E. Saltveit. 1988. Influence of
temperature and controlled atmosphere on storage
Artichokes must be handled with care to of ‘Green Globe’ artichoke buds. HortScience
avoid mechanical damage and therefore limit 23:736-741.
discoloration and pathological problems. During
winter, artichokes may have a white or bronze, Moline, H.E., and W.J. Lipton. 1987. Market
blistered appearance due to being frosted in Diseases of Beets, Chicory, Endive, Escarole,
the field. The artichokes are said to have been Globe Artichokes, Lettuce, Rhubarb, Spinach, and
“frost-kissed.” This is purely an appearance issue Sweet Potatoes. Agriculture Handbook 155, U.S.
and does not affect eating quality. In fact, this Department of Agriculture, Agricultural Research
condition may enhance the nutty flavor. Avoid Service, Washington, DC.
wilted, moldy, significantly discolored, or woody
(overmature) artichokes. Rappaport, L., and A.E. Watada. 1958. Effect of
temperature on artichoke quality. In Proceedings
of the Conference on Transport of Perishables, pp.
Acknowledgments 142-146. University of California, Davis, CA.
Some of the information included was from the Ryall, A.L., and W.J. Lipton. 1979. Handling,
Produce Marketing Association’s “Fresh Produce Transportation and Storage of Fruits and
Manual” and the University of California, Davis, Vegetables, vol. 1, Vegetables and Melons. AVI,
website on “Fresh Produce Facts” at http:// Westport, CT.
postharvest.ucdavis.edu/produce_information.
Ryder, E.L., N.E. DeVos, and M.A. Bari. 1983.
The globe artichoke (Cynara scolymus L).
References HortScience 18:646-653.
Andre, P., R. Blanc, M. Buret, et al. 1980. Trails of Saltveit, M.E. 1991. Artichokes. In P. Lidster,
preservation of artichokes by combined utilization G. Blanpied, and R. Prange, eds., Controlled-
of vacuum precooling, controlled atmospheres, Atmosphere Disorders of Commercial Fruits
and cold. Revue Hort. 211:33-40. and Vegetables, pp. 23-24. Pub. no. 1847/E,
Agriculture Canada, Ottawa, Ontario, Canada.
Bianco, V.V. 1979. Influence of temperature on the
coloration of interior bracts and the inflorescence Saltveit, M.E. 1997. A summary of CA and MA
complex of stored artichokes. In 3rd International requirements and recommendations for harvested
Congress on Artichoke Studies Abstracts, pp. 166. vegetables. In M.E. Saltveit, ed., 7th International
Bari, Italy. Controlled Atmosphere Research Conference,
University of California, Davis, July 13-18, 1997,
Escriche, A., F. Artes, and J.C. Marin. 1982. vol. 4, pp. 98-117. University of California, Davis,
Conservation d’artichauts en atmosphere CA.
controlée. In Progress in the Design and Operation
of Refrigerating Equipment in the Storage of Suslow, T., and M. Cantwell. 1998. Globe
Fruits and Vegetables by Refrigeration, pp. 336- artichoke in “Fresh Produce Facts.” At http://
341. Inst. Intl. du Froid, Paris, France. postharvest.ucdavis.edu/produce_information.
204
Asian Pear Quality Characteristics and Criteria
205
be confined to the use of single trays stacked Respiration Rates
together in a large bin rather than volumes of fruit
packed into large trays or bins. Once the fruit are Temperature mg CO2 kg-1 h-1
harvested, they should be placed in the shade and 0 °C 2 to 8
not left in direct sunlight. 20 ºC 20 to 30
206
Exposure of climacteric cultivars to <1 µL L-1 way down to the calyx. Cavities are usually dry
ethylene accelerates loss of green color and and surrounded by apparently healthy tissue. This
slightly increases softening at 20 °C (68 °F). The disorder can occur in fruit while still on the tree.
effects at 0 °C (32 °F) are minimal. It is more obvious, however, after 2 to 6 weeks in
cold storage. The cause of FSD is still unknown.
However, climatic factors, such as a fluctuating
Physiological Disorders hot and cool summer or high rainfall right before
harvest, may enhance the incidence of this
Internal breakdown and chilling injury can be a disorder. There is no effective way to control FSD
problem with Chinese pear cultivars such as ‘Ya since definite causes have not been identified. The
Li,’ ‘Daisui Li,’ ‘Seuri,’ ‘Tse Li,’ ‘Shin Li,’ and problem is the inability to predict FSD without
Korean pears such as ‘Shingo,’ ‘Okysankichi,’ and cutting the fruit. Further research needs to be done
‘Dan Be.’ Internal browning or core breakdown to determine the causes, variety of susceptibility
is the main worldwide consumer complaint. in local climates, and other control methods,
Development of brown to dark-brown water- either preharvest or postharvest, that will reduce
soaked areas in the core and/or flesh occurs during FSD symptoms to a commercially acceptable
storage, with no visible external indication of level. Meanwhile, whenever possible, avoid the
internal browning. following conditions that might induce FSD: low
crop load (large fruit), later picking (advanced
Fruit grown under the hot conditions of maturity), extreme temperature changes during
California’s San Joaquin Valley and picked the maturation season, sunburn, erratic irrigation
later than 180 days after full bloom are likely or precipitation (frequency, amount, and timing),
to develop browning during storage. In order harvesting fruit under warm temperatures, and
to apply this information to other locations or cooling the fruit rapidly.
years, fruit should be picked no later than 3,000
degree-days as calculated by averaging the daily Low-O2 injury manifests itself as discolored
maximum and minimum temperatures, subtracting surface depressions from exposing ‘Nijisseiki’
4 and summing the resulting values (Johnson and pears to ≤1% O2 for 4 mo at 0 °C (32 ºF) and from
Lakso 1985). The fruit should be picked when exposing ‘Ya Li’ and ‘Tsu Li’ pears to ≤1% O2 for
most of the pears on the tree are still green, though 2 mo, ≤2% O2 for 4 mo, or ≤3% O2 for 6 mo at 0
it is all right if a few at the top have begun to °C (32 ºF).
develop some light-yellow spots. Fruit picked
when the skin is completely yellow will develop High-CO2 injury is manifested as core or medial
internal browning within 1 mo after harvest. In flesh browning, with cavities developing in severe
China, reduction of ‘Ya Li’ internal breakdown has cases as a result of desiccation of dead tissue. ‘Ya
been accomplished using a temperature program Li’ pears can exhibit CO2 injury after exposure to
that decreases storage temperature gradually. ≥5% CO2 for 6 weeks at 0 ºC (32 ºF).
Flesh spot decay (FSD) can be a problem with Watercore symptoms (glassy, diffuse water-soaked
Japanese pear cultivars. FSD is more frequent on areas in flesh; affected areas may taste sweet and
large (<300 g) and overmature fruit. FSD limits turn slightly brown) occur in some cultivars (for
opportunities to grow and market Japanese pears. example, ‘Nijisseiki,’ ‘Shinseiki,’ and ‘Hosui’)
Symptoms include partial browning of spots and under conditions favoring vigorous tree growth.
development of cavities in the flesh. It appears Avoid harvesting overmature fruit to reduce
along and around the vascular bundles when the watercore incidence and severity.
symptoms are severe, but there is no external
indication of the disorder. Generally, FSD is more Superficial scald or skin browning can occur
pronounced above the equator of the fruit (towards after long-term (>16 weeks) storage of ‘Shinsiki’
the stem end), but it can also be observed all the and ‘Nijisseiki’ fruit. Long-term storage can
207
lead to the development of a skin disorder that “Excerpt” to track phytosanitary requirements for
is characterized by the appearance of scaldlike each country. APHIS also provides phytosanitary
browning symptoms. Initially, the affected areas inspections and certifications that declare fruit are
of the skin are light brown in color, but as the free of pests to facilitate compliance with foreign
disorder progresses the skin becomes dark brown regulatory requirements. Issues associated with
and develops a bronze, scaldlike appearance. exotic pest quarantines, addressing both imported
The disorder is confined to the skin and is similar and exported fruit, can change rapidly.
to superficial scald in apples. Scald appears to
be a problem associated with packaging in that
most of the scald appears at the calyx end or that Suitability as Fresh-Cut Product
portion of the fruit that is tightly confined within
the pocket of the pocket pack. However, the Preliminary research conducted in the laboratory
whole fruit surface is susceptible to the disorder, of Dr. Adel Kader at the University of California,
which is rapidly induced if Asian pears are stored Davis, CA in 2000 showed that Asian pear slices
together with apples. To avoid the disorder, have a very short shelf-life, even at 0 to 2 °C (32
adequate ventilation during storage and storage of to 36 °F), due to tissue browning. Dipping slices
fruit in a relatively ethylene-free atmosphere are in 1% ascorbate + 1% CaCl2 for 2 min soon after
recommended. slicing delayed browning, but shelf-life was less
than 5 days at 2 °C (36 °F).
Postharvest Pathology
Special Considerations
Asian pear fruit are susceptible to many
pathogens, such as Botrytis, Alternaria, and The five varieties ‘Shinsui,’ ‘Shinseiki,’
Phomopsis species. These pathogens invade fruit ‘Kosui,’ ‘Hosui,’ and ‘Nijisseiki’ all have an
through wounds caused by mishandling of fruit adequate storage life. If fruit are harvested at the
after harvest. The rots develop slowly in fruit recommended maturity, a storage life of 12 to
during storage and eventually the whole fruit 20 weeks and a subsequent shelf-life of 10 to 15
becomes affected. Affected flesh areas become days can be expected, depending on the variety.
soft and discolored. Damage to fruit surfaces by The major limitation to the storage life of Asian
birds while fruit are on the tree can provide entry pears is development or enhancement of maturity-
points for pathogens. related disorders.
Limited import (Chile) and export (Canada) Most of this information was taken from the
activity is occurring. If imported from Chile, University of California, Davis, website “Fresh
Asian pear shipments must be accompanied by Produce Facts” at http://postharvest.ucdavis.edu/
a PPQ (Plant Pest Quarantine Form 203) signed produce_information.
by the USDA, Animal Plant Health Inspection
Service (APHIS) inspector on site in Chile. If
shipments were not precleared at origin, sampling References
at arrival time is necessary. APHIS issues rules
regarding import requirements and provides Beutel, J. 1990. Asian pears. In J. Janick and J.E.
information to assist exporters in targeting markets Simon, eds., Advances in New Crops, pp. 304-
and defining what entry requirements a particular 308. Timber Press, Portland, OR.
country has. In cooperation with the State plant
boards, APHIS developed a database called
208
Crisosto, C.H., K.R. Day, S. Sibbett, et al. 1994.
Late harvest and delayed cooling induce internal
browning of ‘Ya Li’ and ‘Seuri’ Chinese pears.
HortScience 29:667-670.
209
Asparagus tightly closed. Tender, immature (that is, shorter)
asparagus may be harvested for special markets.
Yaguang Luo, Trevor Suslow, and Marita Cantwell
Luo is with the Food Quality Laboratory, Henry A. Grades, Sizes, and Packaging
Wallace Beltsville Agricultural Research Center,
Agricultural Research Service, U.S. Department of Harvested spears are prepared for market by
Agriculture, Beltsville, MD; Suslow and Cantwell grading, sizing, and bunching. Grades are based
are with the Department of Plant Sciences, on freshness, length and diameter of the stalks,
University of California, Davis, CA. color of spears, tightness of the spear tips, and
the extent of bruising. Sizing is based on spear
diameter with U.S. No. 1 having a diameter >1
Scientific Name and Introduction cm (>0.5 in) and U.S. No 2 having a diameter
>0.8 cm (>5/16 in). Spears of larger diameter
Asparagus (Asparagus officinalis L.) is a perennial are considered to be superior in quality with less
of the Liliaceae family. The edible portion of fiber (Peirce 1987). Spears are tied in bunches
asparagus is a rapidly growing stem (shoot) weighing 0.45 to 1.1 kg (1 to 2.5 lb) and trimmed
with scale leaves that arise at nodes. There are to a standard length of 18 to 25 cm (7 to 10 in).
two forms of marketed asparagus, namely white Color is also important, with U.S. No. 1 spears
(blanched) and green. White asparagus is more being green for more than 2/3 their length, while
common in Europe and Asia (Lipton 1990); U.S. No. 2 are green for more than half their
green asparagus is popular in the United States length. After trimming the butt-end, the bunches
and is produced predominantly in California and are packed upright in trapezoidal-shaped crates to
Washington. Asparagus has a high metabolic rate minimize geotropic bending (curving away from
after harvest and is among the most perishable gravity) in transit. The container should include a
crops. wet pad in contact with the butt end to maintain
turgidity. Headspace is provided in the carton to
prevent tip curvature or breakage during spear
Quality Characteristics and Criteria elongation.
210
temperature is critical to delay senescence, tissue Chilling Sensitivity
toughening, and flavor loss (King et al. 1993).
High RH is essential to prevent desiccation and to Asparagus is subject to chilling injury after about
maintain freshness. Typically, asparagus is packed 10 days at 0 °C (32 °F). Symptoms include loss
and shipped with water-saturated pads in the of sheen and glossiness and graying of tips. A
bottom of the containers to maintain high RH and limp, wilted appearance may be observed. Severe
to replenish water lost by the spear or water used chilling injury may result in darkened spots or
during spear elongation; this practice maintains streaks near the tips.
spear turgidity. Excessive free water at elevated
storage or shipping temperatures may lead to
increased decay. Ethylene Production and Sensitivity
212
Hernandez-Rivera, L., R. Mullen, and M. Saltveit, M.E. 1997. A summary of CA and MA
Cantwell. 1992. Textural changes of asparagus requirements and recommendations for harvested
in relation to delays in cooling and storage vegetables. In M.E. Saltveit, ed., 7th International
conditions. HortTech. 2:378-381. Controlled Atmosphere Research Conference,
University of California, Davis, July 13-18, 1997,
King, G.A., D.C. Woollard, D.E. Irving, and W.M. vol. 4, pp. 98-117. University of California, Davis,
Borst. 1990. Physiological changes in asparagus CA.
spear tips after harvest. Physiol. Plant. 80:93-400.
Saltveit, M.E., and R.F. Kasmire. 1985. Changes
King, G.A., P.L. Hurst, D.E. Irving, and R.E. in respiration and composition of different length
Lill. 1993. Recent advances in the postharvest asparagus spears during storage. HortScience
physiology, storage and handling of green 20:1114-1116.
asparagus. Postharv. News Info. 4(3):5N-89N.
Snowdon, A.L. 1992. A Color Atlas of Postharvest
Klieber, A., and R.B.H. Wills. 1992. Optimization Diseases and Disorders of Fruits and Vegetables,
of storage conditions for ‘UC 157’ asparagus. vol. 2, CRC Press, Boca Raton, FL.
Australian J. Expr. Agric. 32:529-534.
Suslow, T.V. 1998a. Asparagus Producers
Lill, R.E., and V.K. Corrigan. 1996. Asparagus and Shippers Quality Assurance Guidance to
responds to controlled atmospheres in warm Minimizing Microbial Food Risks. University of
conditions. Intl. J. Food Sci. Tech. 31:117-121. California Vegetable Research and Information
Center, Davis, CA, and the California Asparagus
Lill, R.E., G.A. King, and E.M. O’Donoghue. Commission, El Centro, CA.
1990. Physiological changes in asparagus spears
immediately after harvest. Scientia Hort. 44:191- Suslow, T.V. 1998b. Asparagus produce facts.
199. Recommendations for Maintaining Postharvest
Quality. At http://postharvest.ucdavis.edu/
Lipton, W.J. 1990. Postharvest biology of fresh produce_information.
asparagus. Hort. Reviews. 12:69-155.
Suslow, T. 2001. Progress note on asparagus
Lipton, W.J. 1957. Physiological changes in handling and display. University of California
harvested asparagus (Asparagus officinalis) Perishables Handling Quarterly, no. 106. At http://
as related to temperature of holding. Ph.D. postharvest.ucdavis.edu.
Dissertation, University of California, Davis, CA.
Torres-Penaranda, A.V., and M.E. Saltveit. 1994.
Paull, R.E., and N.J. Chen. 1999. Heat treatment Effects of brief anaerobic exposures on carbon
prevents postharvest geotropic curvature of dioxide production and quality of harvested
asparagus spears (Asparagus officinalis C.). asparagus. J. Amer. Soc. Hort. Sci. 119:51-555.
Postharv. Biol. Technol. 16:37-41.
213
Atemoya Precooling Conditions
Robert E. Paull and Nancy Jung Chen Room or forced-air cooling to 10 to 13 °C (50 to
55 ºF).
Paull and Chen are with the Department of
Tropical Plant and Soil Sciences, University of
Hawaii at Manoa, Honolulu, HI. Optimum Storage Conditions
Physiological Disorders
Postharvest Pathology
Quarantine Issues
References
215
Avocado Avocados are available year-round in the United
States and are supplied by two major producing
Allan B. Woolf, Anne White, Mary Lu Arpaia, and areas: California (90%) and Florida (10%).
Kenneth C. Gross Fruit (mainly ‘Hass’) are also imported from (in
approximate order by volume) Chile, Mexico, the
Woolf and White are with the Mt. Albert Research Dominican Republic, New Zealand, Bahamas, and
Centre, Plant and Food Research, Auckland, NZ; Jamaica.
Arpaia is with the Department of Botany and
Plant Sciences, University of California Kearney There are many cultivars of avocados grown
Agricultural Center, Parlier, CA; Gross is formerly commercially in the United States, and they
with the Food Quality Laboratory, Henry A. come in assorted sizes and shapes. The primary
Wallace Beltsville Agricultural Research Center, California cultivar is Hass, a G-MX hybrid that
Agricultural Research Service, U.S. Department accounts for approximately 95% of the planted
of Agriculture, Beltsville, MD. He is now retired. acreage. Other cultivars include ‘Bacon,’ ‘Fuerte,’
‘Gwen,’ ‘Lamb Hass,’ ‘Pinkerton,’ ‘Reed,’ and
‘Zutano.’ With the exception of ‘Reed,’ which
Scientific Name and Introduction is believed to be entirely of the G race, the other
cultivars are considered to be primarily G-MX
The avocado originated in Central America and hybrids. ‘Hass’ accounts for 80% of avocado
southern Mexico. The Aztecs considered the consumption in the United States and is the main
avocado an aphrodisiac and called it “huacatl.” In focus of research and development. The main
Chile, Peru, and Ecuador, it is called “palta,” an Florida cultivars (West Indian and Guatemalan
Incan name. Spanish-speaking people also call it races and hybrids) are ‘Simmonds,’ ‘Nadir,’
“aguacate,” “cura,” or “cupandra.” The avocado ‘Booth 8,’ ‘Choquette,’ and ‘Lula.’
is known as “abogado” in Spain. Historically, the
avocado has also been referred to as “alligator
pear,” “vegetable butter,” “butter pear,” and Quality Characteristics and Criteria
“midshipman’s butter.”
For avocado, the major quality criteria used
The avocado is botanically classified into three during grading are size and skin color, as well as
races: (1) West Indian (WI), Persea americana freedom from wounds, blemishes, insect damage
Mill. var. americana (P. gratissima Gaertn.), (particularly from caterpillar and thrip scarring),
tropical with large variably shaped fruit and lower spray residues (most commonly copper), and
oil content; (2) Mexican (MX), P. americana Mill. other contaminants on the skin. When ripe, the
var. drymifolia Blake (P. drymifolia Schlecht. key issues are absence of disease (body rot and
& Cham.), semitropical with smaller elongated, stem end rots), physiological disorders (flesh
thin-skinned fruit and higher oil content; and (3) graying), and physical damage (bruising). Many
Guatemalan (G), P. nubigena var. guatemalensis of these quality factors depend on the cultivar, and
L. Wms., subtropical with mostly round, thick- consumer preference for size, shape, and color can
skinned fruit and intermediate oil content (Bergh vary from region to region.
and Lahav 1996). Many of the commercial
cultivars are hybrids of these three races. There While avocados from both Florida and California
is great variability in fruit traits not only between are high-quality fruit, there are significant
races but between cultivars within a race. One of differences in size, texture, and flavor. Florida
the most distinct differences between cultivars avocados are usually at least twice as large as
is the peel color when ripe. The peel of some those from California and often sell at a lower
cultivars changes from green to black or purple price. The smaller California avocados have a
with increasing maturity or ripening. desirable nutlike flavor and a richer, creamier
texture than the less oily Florida fruit, which are
216
sometimes marketed as “lite avocados.” These (18.7%), and ‘Zutano’ (18.7%). In California, fruit
differences are mainly due to the fact that different are also released into the market at predetermined
horticultural races of the avocado are produced in dates based on dry matter and size for each
California and Florida. cultivar. For example, the size and release dates
for ‘Hass’ are size 40 and greater on November
Avocados are one of the few fruit that contain 28; Size 48, December 12; Size 60, January 2; and
significant quantities of oil; sometimes more than Size 70 or smaller, January 16. Florida avocados
30% of fresh weight, depending on cultivar and have lower oil content (3 to 15%) and are
maturity. Oil content is a key part of the sensory generally harvested at a specific date and weight
quality. Oil quality is very similar to that of olive or size.
oil, with approximately 75% monounsaturated,
15% saturated, and 10% polyunsaturated fatty Avocados can be held on the tree for many months
acids (omega-6). However, there is variation with after they are physiologically mature because they
race, cultivar, growing region, and season. The do not ripen until they are harvested. However,
high-monounsaturated, high-polyunsaturated, time to ripen does decrease with increasing time
and low-saturated fatty acid content makes this a on the tree. Freshly harvested avocados tend to
“healthy” oil in terms of effect on heart disease. have “green” skins, though ‘Hass’ fruit that are
In addition, avocado oil contains a range of other harvested late in the season may have some skin
health-promoting compounds such as chlorophyll, darkening at harvest. The peel of ripe ‘Hass’ and
carotenoids, α-tocopherol, and β-sitosterol. ‘Lamb Hass’ avocados should have a dark, purple-
These health factors, along with the absence of black or black skin, while green-skinned cultivars
cholesterol, should be emphasized with consumers remain green when ripe. Avocados are ripe when
since avocados are perceived by some as an the fruit yields slightly to light finger pressure.
unhealthy or “fat” fruit. Extraction of oil from Pulp color, texture, and flavor when ripe are
reject avocados is carried out in some countries cultivar specific.
for use in cosmetic products and for culinary
purposes.
Grades, Sizes, and Packaging
Traditionally, fruit produced in the United States
have been “clip” harvested (peduncle cut to leave California avocados are packed in single-layer
a “button” at the top of the fruit). However other 12.5 lb (5.67 kg) flats or trays, 2-layer 25 lb (11.34
producing countries (Australia, Israel, Spain, and kg) lugs, and 25 lb (11.34 kg) volume-fill boxes.
South Africa) are now “snap”-harvesting ‘Hass’ RPCs (returnable plastic containers) are used
fruit. The ultimate success of snap harvesting increasingly. There is also increased usage of
depends on fruit maturity, growing conditions prepacked units such as polyethylene containers
(rain), and cultivar (Arpaia and Hofshi 1998). (“clam shells”) or mesh bags. For Florida
avocados, the common packages used are single-
layer, 13 lb (6.12 kg) flats; two-layer, 27 lb (12.47
Horticultural Maturity Indices kg) lugs; 35 lb (15.88 kg) cartons; and 10 lb (4.54
kg) natural packs.
The percentage of dry matter in avocado fruit
is highly correlated to oil content and is the key California avocados are graded as No. 1 or No. 2.
maturity index used in California and worldwide Florida avocados are graded as U.S. No. 1, U.S.
(Lee et al. 1983). Minimum dry matter percentage No. 2, and U.S. Combination. However, only
ranges from 17 to 25%, depending on cultivar. In some Florida varieties are graded, while the others
California, the minimum percentage of dry matter are marketed as unclassified. In California, fruit
at harvest for the major cultivars are ‘Bacon’ are weight-sized into the following categories:
(17.7%), ‘Fuerte’ (19.0%), ‘Gwen’ (24.2%), 20 (18.75 to 22.0 oz, 532 to 624 g), 24 (15.75 to
‘Hass’ (20.8%), ‘Pinkerton’ (21.6%), ‘Reed’ 18.75 oz, 447 to 532 g), 28 (13.75 to 15.75 oz, 390
217
to 447 g), 32 (11.75 to 14.0 oz, 333 to 397 g), 36 result from suboptimal storage temperatures.
(10.5 to 12.5 oz, 298 to 354 g), 40 (9.50 to 11.50 Therefore, it is important to maintain the
oz, 269 to 326 g), 48 (7.50 to 9.50 oz, 213 to 269 appropriate temperature for unripe fruit and stack
g), 60 (6.25 to 7.50 oz, 177 to 213 g), 70 (4.75 to containers to allow for proper air circulation and
6.25 oz, 135 to 177 g), and 84 (3.75 to 4.75 oz, temperature control.
106 to 135 g) count for 25 lb packs, and half these
values for flats (or single-layer trays). Florida
fruit are packed by count. Regulations specify that Controlled Atmosphere (CA)
the pack shall be at least fairly tight and that the Considerations
weight of the smallest fruit in any container shall
not be less than 75% that of the largest fruit in the CA storage is commonly used for transporting
same container. Commonly used sizes for Florida fruit to distant markets in refrigerated shipping
packages are 6, 7, 8, 9, 10, 12, 14, 16, 18, 20, and containers. The atmosphere used and technology
24 count. for controlling the atmosphere vary between
shipping companies. Generally, O2 levels of 2 to
5% (or possibly as high as 10%) + 3 to 10% CO2
Precooling Conditions are used. The primary benefit of low O2 is that
of delayed softening and reduction of respiration
Ripening and associated softening of avocados and ethylene production from standard storage
can be delayed by cooling soon after harvest. This temperatures. Elevated CO2 may delay softening
is critical where long storage periods are required and reduce sensitivity to external chilling injury
or where field temperatures are above 25 °C (77 and allow lower storage temperatures (Faubion et
°F). Forced or passive air-cooling is generally al. 1992). Low-O2 injury may appear as irregular
carried out as rapidly after harvest as possible; brown to dark brown patches on the skin and
within 24 to 48 h or sooner is recommended. may additionally cause diffuse browning of
Hydrocooling of ‘Hass’ is also used commercially. flesh beneath affected skin. CO2 atmospheres
Recommendations as to the target temperature above 10% can be detrimental by leading to
(prior to packing) vary between 5 and 15 °C discoloration of the skin and development of off
(41 and 59 °F) and may be influenced by the flavor, particularly when the O2 concentration is
emphasis on whether fruit should be graded with less than 1%. Reducing ethylene levels to below 1
condensation or not. µL L-1 using ethylene scrubbers during CA storage
may provide added benefits for retarding ripening
and decrease development of chilling injury
Optimum Storage Conditions (Faubion et al. 1992).
218
(ppb) at about 6 °C (43 °F) for 18 to 24 h. For to reduce the proportion of fruit that become
‘Hass,’ 1-MCP treatments will have benefit if overripe, with concomitant increase in rots and
fruit are stored for longer than 4 weeks. However, other disorders. Avocados should not receive a
for other cultivars that exhibit higher levels of water sprinkle or top ice.
internal chilling injury at even short storage times,
1-MCP may be of more benefit. Care should be
taken because applying higher concentrations Chilling Sensitivity
may result in excessive delays to softening and
ripening, which in turn are likely to increase Avocados exhibit two forms of chilling injury,
disease incidence. 1-MCP does not reduce internal and external, which are generally induced
external chilling injury (skin blackening) of ‘Hass’ by quite different storage conditions. Internal
avocados. chilling injury manifests as a grayish-brown
discoloration of the flesh, particularly at the base
Step-Down Temperatures. The other key of the fruit around the seed. This can be associated
technology for maintaining fruit quality with vascular browning, which starts at the base
(particularly preventing internal chilling injury), of the fruit (rather than at the stem end, which is
proven over many years by the South African often associated with stem end rots). In ‘Hass,’
industry, is the use of “step-down” temperatures internal chilling injury tends to occur after about
(Vorster et al. 1987). Temperatures are typically 4 or more weeks in storage at about 6 °C (43 °F),
decreased 1 to 2 °C each week during shipping, but will be influenced by maturity and growing
with the final temperature not to drop below 3.5 conditions. Cultivars differ in their susceptibility
°C (38 °F), and with progressively lower initial to this disorder, with some being very sensitive.
temperatures as fruit maturity increases. These Calcium levels are a possible explanation for
temperature regimes have been developed and differences in internal chilling injury (Chaplin and
refined over many years and have resulted in a Scott 1980). Another low-temperature disorder,
protocol for each cultivar for differing times in the “pulp spot,” may be observed in ‘Fuerte’ fruit
season and growing region. in which small dark spots can be observed in
the flesh. Internal chilling injury is generally
associated with softening of fruit during storage
Retail Outlet Display Considerations and thus is increased by the presence of ethylene
(Chaplin et al. 1983). Internal chilling injury is
Avocados are best ripened at 15 to 20 ºC (59 to the key limiting factor for long-term storage of
68 ºF) (Hopkirk et al. 1994). The ripening rate avocados.
at temperatures below 15 ºC (59 ºF) is relatively
slow, and ripening at temperatures above 25 ºC External chilling injury occurs as irregular
(77 ºF) may result in increased decay, uneven patches of blackening on the skin (similar to
ripening of the flesh, and off flavors. As for apple scald) that can be observed during storage
bananas, ethylene treatment can be used to but that generally increase slightly in intensity
“precondition” or “preripen” fruit. Avocados are after removal from cold storage. The damage is
very susceptible to bruising during softening first seen in inner cell layers of the exocarp and
(Arpaia et al. 1987) and thus should be handled then the outer layers of the skin (Woolf 1997). In
carefully during transport and display. Any means cultivars that naturally darken during ripening,
of minimizing “squeezing” by customers will such as ‘Hass,’ the damage will be less apparent
also improve quality. Since quality can decrease after ripening but may be distinguished as brown,
rapidly during softening, it is best to check corky skin tissue in ripe fruit. External chilling
avocado ripeness every day and to display or use injury is generally induced by temperatures below
the ripest fruit first. If possible, ripe or near-ripe 3 °C (38 °F). However, fruit become less sensitive
fruit should be held at lower temperatures (1 to 6 with increasing maturity, and ripe fruit are less
°C, 34 to 43 °F) (Young and Kosiyachinda 1976) affected. Fruit exposed to low temperatures may
219
be of poor internal quality when ripe, with a high fruit), since the time to ripen decreases during
incidence of rots and softening disorders (Woolf et storage. Because heat production of avocados
al. 1995), but will have lower incidence of internal is much greater than that of many other fruit
chilling injury (graying). For ‘Hass’ fruit stored crops, careful attention should be paid to air flow
for long periods at standard storage temperatures and temperature management during ethylene
(for example, 6 to 7 weeks at about 6 °C [43 °F]), treatment and subsequent ripening. Palletized fruit
a form of external chilling injury is expressed that may reach temperatures of more than 30 °C (86
is of a very similar appearance to that observed at °F), with negative effects on ripe fruit quality. For
low temperatures. This form of external chilling this reason, ethylene treatment of palletized fruit
injury will tend to be seen in fruit that are quite should be carried out under forced-air conditions.
soft (nearly ripe) at the point of removal from During ethylene treatment, CO2 levels should be
storage. maintained at less than 1 to 2% by either continual
venting of the atmosphere or full venting and
ethylene reinjection if “shot” systems are used.
Ethylene Production and Sensitivity Following ripening, fruit should be precooled to 5
°C (41 °F).
Rates of ethylene production are generally low
for unripe avocados, less than 0.1 μL kg-1 h-1 at 20
ºC (68 ºF), but increase rapidly after harvest up to Respiration Rates
levels greater than 100 μL kg-1 h-1 at 20 ºC (68 ºF)
when fully ripe. Therefore, ripe avocados should Respiration rate of avocados is relatively high
not be stored with fruits and vegetables that are compared with many other fruit crops.
sensitive to ethylene damage. Unripe avocados
are very sensitive to ethylene. They should not Temperature mg CO2 kg-1 h-1
be stored near ripe fruit or other fresh produce 5 ºC 20 to 50
that produces more than trace ethylene. Ethylene 10 ºC 50 to 160
exposure during storage accelerates ripening and 20 ºC 80 to 300
softening and can increase incidence and severity Data from Kader and Arpaia (2001).
of internal chilling injury and decay.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
Ethylene Treatment (“Preconditioning” or 1.8 at 20 °C (68 °F). To calculate heat production,
“Preripening”) multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal per tonne per day.
There is an increasing move at the retail level
toward “ripe for tonight” programs that generally
result in significant increases in sales. This is Physiological Disorders
achieved by treating avocados with 10 to 100
µL L-1 ethylene at 17 to 20 ºC (63 to 68 ºF) for There is a range of physiological disorders of
approximately 48 to 72 h (early season), 24 to 48 avocados (Arpaia et al. 2009), and most of these
h (mid season), or 12 to 24 h (late season). This occur following long storage periods. The key
significantly reduces both the time to ripen (to 3 disorders are flesh graying, vascular browning,
to 6 days, depending on cultivar and maturity) and pulp spot, which are all symptoms of internal
and fruit-to-fruit variability in ripening. If fruit are chilling injury (see Chilling Sensitivity). If fruit
stored prior to ethylene treatment, the duration of are stored for excessively long periods, the
treatment required to achieve maximum rate of flesh may also fail to ripen evenly and become
ripening is reduced. For ‘Hass,’ after 3 to 4 weeks increasingly susceptible to pathogens. The timing
of storage, there may be relatively little benefit to of expression of internal chilling injury and its
ethylene treatment (particularly for later-season severity depend on temperature management,
220
initial ripeness, cultivar, production area, and fruit reduce rots. Snap picking of fruit can reduce stem
maturity. External chilling injury may occur if end rot incidence in dry periods, but it can result
fruit are stored at low temperatures (0 to 3 °C [32 in increased rots in humid growing environments
to 38 °F]) or for long periods (more than 6 weeks) or when fruit are harvested in wet conditions.
at standard storage temperatures.
Perhaps the most important area for reducing rots
is that of maintaining good ripe-fruit quality by
Postharvest Pathology optimizing temperatures during handling, storage,
transport, and ripening. It is also critical not to
Rots of avocados are divided into two categories store fruit for long periods. Ripening fruit at lower
on the basis of their location (Snowdon 1990). temperatures—for example, 15 to 20 °C (59 to
Stem end rots enter the fruit at the stem, or 68 °F)—can lead to significant reduction in rots
peduncle end of the fruit, and move down the fruit compared with higher temperatures (Hopkirk et
resulting in discolored flesh, often with associated al. 1994). Storing ‘Hass’ fruit for 1 to 3 weeks
browning of the vascular strands (Johnson and can also reduce rot incidence (as compared with
Kotze 1994). Body rots invade through the skin nonstored fruit), as can ethylene treatments, which
and are generally manifested as circular brown to both synchronize and hasten ripening. Postharvest
black spots that may be covered with spore masses fungicides (prochloraz, benlate [benomyl], and
in the later stages of infection. Decay penetrates thiabendazole) are used in some countries, but
through to the flesh resulting in discrete areas of these are not registered for use in the United
discolored flesh. In cultivars that darken when ripe States (Darvas et al. 1990). Research on biological
(‘Hass’), rots may be less obvious externally. Rots control agents is being carried out in South Africa,
are rarely observed at harvest or during storage but New Zealand, and Australia.
can increase rapidly with fruit softening. Where
infection pressure is high and physical damage to
the skin occurs prior to storage, small, soft-black Quarantine Issues
circles of infection (“measles”) can occur during
storage. These generally spread rapidly outwards Issues relating to quarantine will vary according
after removal from storage. to the marketplace and country of origin, and
guidance should be sought from the Department
The causal organisms can vary with growing of Agriculture. If avocados are grown in fruit-fly-
environment and country. The following infested areas, significant quarantine issues will
pathogens (in order of frequency) have been arise. Methyl bromide treatment is an APHIS-
isolated from decayed California avocados: approved treatment for Mediterranean fruit fly,
Colletotrichum, Dothiorella, Alternaria, and but it results in a significant reduction in fruit
Phomopsis spp. (Smilanick and Margosan 2001). quality. Because avocados do not tolerate standard
Differences in the pathogens responsible for decay high-temperature disinfestation treatments, (for
exist among countries; for example, New Zealand example, fruit core temperatures of 47 °C [117
versus Australia (Everett 1996, Arpaia et al. 2009). °F] for 20 min), low-temperature disinfestation
is the most viable approach. Tolerance to
Preharvest control methods for postharvest fungal temperatures that can be used for low-temperature
decay include good orchard sanitation (removal disinfestation can be imparted by pretreatments
of mummified fruit and dead wood) and effective at 38 °C (100 °F) (Sanxter et al. 1994, Woolf et
preharvest fungicide application, such as copper, al. 1995), for example, or by low-temperature
which is widely used in some countries (including conditioning (Woolf et al. 2003). However, the
the United States, in Florida) where humid only commercial disinfestation treatment in use is
growing conditions prevail. Harvesting should not for Queensland fruit fly in ‘Hass’ avocados: 6 to
be carried out in the rain or when fruit are wet, and 8 °C (43 to 47 °F) for 3 to 5 days followed by 16
careful handling to minimize skin damage helps days at temperatures <1 °C (34 °F) (Hofman et al.
221
2003). However, this low-temperature disinfesting Bergh, B.O., and E. Lahav. 1996. Avocados. In J.
treatment may not be effective for all fruit fly Janick and J.N. Moore, eds., Fruit Breeding, vol. I,
species, since cold tolerance varies. Tree and Tropical Fruits, pp. 113-166. John Wiley
& Sons, New York, NY.
Suitability as a Fresh-Cut Product Chaplin, G.R., and K.J. Scott. 1980. Association
of calcium in chilling injury susceptibility in
Avocados are not currently marketed as fresh-cut stored avocados. HortScience 15:514-515.
products, but they are marketed as chunks, paste,
and guacamole dips. Chaplin, G.R., R.B.H. Wills, and D. Graham.
1983. Induction of chilling injury in stored
avocados with exogenous ethylene. HortScience
Acknowledgments 18:952-953.
Some of the above information was taken from the Darvas, J.M., J.M. Kotze, and F.C. Wehner.
previous edition of Agriculture Handbook Number 1990. Effect of treatment after picking on the
66; the Produce Marketing Association’s Fresh incidence of postharvest diseases of avocado.
Produce Manual; the University of California, Phytophylactica 22:93-96.
Davis, website “Fresh Produce Facts” (http://
postharvest.ucdavis.edu/produce_information); the Everett, K.R. 1996. Postharvest diseases
Michigan State University Extension Information of avocados. At http://www.hortnet.co.nz/
Management Program Database website (http:// publications/science/everavo1.htm.
msue.anr.msu.edu); the California Avocado
Commission website (http://www.avocado.org); Faubion, D.F., F.G. Mitchell, G. Mayer, and M.L.
and the University of Florida website (http:// Arpaia. 1992. Response of ‘Hass’ avocado fruit
edis.ifas.ufl.edu). The authors also express their to postharvest storage in controlled atmosphere
appreciation for input and comments on the conditions. In C.J. Lovatt, and P. Holthe, eds.,
manuscript by Adel Kader, Joe Smilanick, Reuben Proceedings of 2nd World Avocado Congress,
Hofshi, Chris Yearsley, Laurence Eyers, Ian Orange, CA, April 1991, pp. 467-472. University
Ferguson, and Linda Boyd. of California, Riverside, CA.
222
Kader, A.A., and M.L. Arpaia. 2001. Avocado. Woolf, A.B. 1997. Reduction of chilling injury
Recommendations for maintaining postharvest in stored ‘Hass’ avocado fruit by 38 °C water
quality. At http://postharvest.ucdavis.edu/produce_ treatments. HortScience. 32:1247-1251.
information.
Woolf, A.B., C.B. Watkins, J.H. Bowen, et al.
Kosiyachinda, S., and R.E. Young. 1976. Chilling 1995. Reducing external chilling injury in stored
sensitivity of avocado fruit at different stages of ‘Hass’ avocados with dry heat treatments. J. Amer.
the respiratory climacteric. J. Amer. Soc. Hort. Soc. Hort. Sci. 120:1050-1056.
Sci. 101:665.
Woolf, A.B., K.A. Cox, A. White, and I.B.
Lee, S.K., R.E. Young, P.M. Schiffman, and C.W. Ferguson. 2003. Low temperature conditioning
Coggins. 1983. Maturity studies of avocado fruit treatments reduce external chilling injury of ‘Hass’
based on picking dates and dry weight. J. Amer. avocados. Postharv. Biol. Technol. 13:113-122.
Soc. Hort. Sci. 108:390-394.
Young, R.E., and S. Kosiyachinda. 1976. Low
Pesis, E., M. Ackerman, R. Ben-Arie, et al. 2002. temperature storage of ripe avocado fruit. In
Ethylene involvement in chilling injury symptoms California Avocado Society Yearbook, pp. 73-76.
of avocado during cold storage. Postharv. Biol. Temecula, CA.
Technol. 24:171-181.
223
Banana and Plantain Horticultural Maturity Indices
224
preference. Plantains are packed as individual a much fresher condition (latex flowing through
fingers in 18 kg boxes. Most exporters use the vascular tissues) on arrival at destination.
polyethylene film liners and paper pads to reduce Shelf-life can potentially be extended twofold
moisture loss and protect fruit from physical to threefold by optimum CA. O2 levels below
damage during handling and transport. 1 to 1.5% may cause grayish or brown peel
discoloration, failure to ripen properly, and off
flavor (Kader 1992, 1994, 1997). CO2 levels above
Precooling Conditions 6 to 8% may cause pulp to soften while the peel
is still green and may confer undesirable texture
Precooling of bananas or plantains is not generally and flavor (Wei and Thompson 1993, Kader 1994,
done. Adequate cooling is not initiated until fruit Kader 1997). Ripe fruit can tolerate higher levels
are loaded into containers or cargo holds aboard of CO2.
ships.
The beneficial and detrimental effects of reduced
O2 and/or elevated CO2 depend on temperature
Optimum Storage Conditions and time of exposure. Removal of ethylene gas
can have an additional benefit on extending
Optimum temperatures for storage and holding green-life of bananas and plantains under both
of green bananas are 13 to 14 ºC (56 to 58 ambient and modified atmosphere conditions
ºF) (Thompson and Burden 1995). Because (Scott et al. 1970, Liu 1976, Scott 1977, Turner
marketing quality standards are more relaxed 1997). CO2 inhibits the effect of ethylene on
for plantains, and plantains are more prone to ripening, and higher levels of O2 than those under
premature ripening during transit and storage, CA are necessary for adequate ripening. Thus,
it is recommended that green plantains be held bananas held under CA should be ventilated with
between 9 and 12 ºC (48 to 53 ºF). Plantains fresh air for at least 18 to 24 h before ripening
grown during the warmer months tend to attain (gassing with ethylene) is initiated. An underpeel
physiological maturity faster than fruit grown discoloration resembling chilling injury has been
during the winter months; thus green-life potential observed on green bananas when transported long
varies during the year. Optimum RH for holding distances under CA conditions at temperatures
and transporting fruit is 90 to 95%. Holding of below 14 ºC (58 ºF). This disorder has also been
ripe fruit should be kept to a minimum. associated with high temperatures of 33 to 35 ºC
(91 to 95 ºF) in the field (Stover and Simmonds
1987).
Controlled Atmosphere (CA)
Considerations
Retail Outlet Display Considerations
Bananas and plantains respond well to CA. CA
is used commercially during ocean transport of Fruit should be displayed at retail in
green bananas. Modified atmosphere packaging nonrefrigerated areas in the produce section.
using polyethylene bags (banavac) is used for Existing refrigerated shelf space in supermarkets
both bananas and plantains, but plantain are normally is below the minimum safe temperature
physiologically more active than bananas and for bananas and plantains and chilling injury can
have higher respiration rates and so could exhaust still occur in ripe fruit. Displaying surfaces should
O2 more rapidly than bananas. Optimum gas be cushioned to avoid physical damage to the
levels for most cultivars range between 2 to fruit.
5% O2 and 2 to 5% CO2 (Bishop 1990, Kader
1992, Kader 1994, Kader 1997). Main benefits
of controlled atmosphere include delaying of
ripening, reduction of crown rot incidence, and
225
Chilling Sensitivity Controlled ripening. Mature bananas left to ripen
naturally will eventually soften, but the change in
Chilling injury is an important disorder of color will not be uniform and the peel will be dull,
bananas and plantains. Both green and ripe fruit pale yellow, and unattractive. In order for the fruit
are susceptible, with green fruit being slightly to attain a bright yellow peel color, a firm pulp
more sensitive than ripe fruit. Chilling injury texture, and good flavor, bananas are ripened by
results from exposing fruit to temperatures below releasing ethylene into a sealed chamber or room
about 13 ºC (56 ºF) for a few hours to a few days, and at controlled temperature and RH. Plantains
depending on cultivar, maturity, condition of are being ripened by this controlled method in
the fruit, temperature, and duration of exposure most markets, but some markets still rely on
(Stover 1972, John and Marchal 1995, Turner natural ripening. Immediately after harvest,
1997). Chilling injury is mainly a peel disorder. bananas do not respond to ethylene treatment
Symptoms include subepidermal discoloration or, in the best scenario, will initiate ripening but
visible as brown or black streaks in a longitudinal will never attain the characteristic bright yellow
cut, a dull or grayish (smokey) cast on ripe fruit, color. One main reason for controlled ripening is
and failure to ripen. In severe cases, the peel to provide retailers and wholesalers with fruit at a
turns dark brown or black, and even the flesh can stage of ripeness desired by consumers.
turn brown and develop an off taste (John and
Marchal 1995, Turner 1997). Chilled fruit are Generally, very low concentrations of ethylene
more sensitive to mechanical injury. Ripe fruit, are sufficient to ripen the fruit—10 to 50 µL L-1
if chilled, turn dull brown when later exposed to (Thompson and Seymour 1982). In commercial
higher temperatures and are very susceptible to practice, however, 1,000 µL L-1 is commonly
handling marks: The slightest pressure can cause used to ensure uniform ripening. This is partly
discoloration. Inflicted chill in green or ripe fruit because many ripening rooms are not fully gas-
may not become apparent until 18 to 24 h after tight and the concentration may be rapidly reduced
actual damage has occurred. through leakage. Most commercial cultivars
of bananas and plantains require exposure to
ethylene for 24 to 48 h at 14 to 18 ºC (58 to 64 ºF)
Ethylene Production and Sensitivity (Thompson and Burden 1995, Robinson 1996).
However, temperatures of up to 20 ºC (68 ºF) are
Bananas and plantains are sensitive to sometimes necessary (Thompson and Burden
physiological levels of ethylene as low as 0.3 to 1995). Optimum RH levels during ripening are 90
0.5 µL L-1 if the O2 and CO2 levels are similar to to 95%. (After coloring is underway, RH should
those found in outside fresh air (Peacock 1972). be reduced to 85% to prevent peel splitting.)
The three main factors affecting response to High RH requirements for proper ripening can be
external ethylene are fruit maturity, time from attained when the fruit is being packed in partially
harvest when ethylene exposure began, and length sealed polyethylene liners. Exposure of ripe
of exposure to ethylene. bananas or plantains to temperatures higher than
those in the ripening range can hasten softening
Ethylene production rates: and decay, weaken the neck, and cause splitting of
Temperature μL C2H4 kg-1 h-1 the peel and poor color development.
13 ºC 0.04 to 2.0
15 ºC 0.15 to 5.0 The color of the peel is used as an indicator of
18 ºC 0.20 to 8.0 ripening. A scale of 1 to 7 is convenient: 1 is
20 ºC 0.30 to 10.0 dark green, 2 is light green, 3 is more green than
Lower values in the range are for mature-green yellow, 4 is more yellow than green, 5 is yellow
fruit, and higher values are for ripening fruit. with green tips, 6 is fully yellow, and 7 is flecking
Data modified from Kader (1998). (Kader 1992). Room ventilation after gassing
with ethylene is essential to keep CO2 below
226
1% and avoid its effects on delaying ethylene The lower value of the range is for mature-green
action. Use of a forced-air system (pressurized) fruit, and the higher value is for ripening fruit. To
in ripening rooms assures more uniform cooling get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate by
or warming of the fruit as needed and more 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and 1.8
uniform ethylene concentrations throughout the at 20 °C (68 °F). To calculate heat production,
ripening. When ripening is done in nonpressurized multiply mg kg-1 h-1 by 220 to get BTU per ton per
conventional rooms, open stacking of boxes is day or by 61 to get kcal per tonne per day.
essential to allow adequate air circulation for
uniform ripening. Many stacking patterns are
used and the best pattern to be used depends on Physiological Disorders
pallet sizes and ripening facilities. Because heat
rises, the amount of box-op area exposed is most A condition known as “maturity bronzing” or
important for preventing heat buildup in the stack “maturity stain” has been observed in Australia
and controlling pulp temperature during ripening. and Central America at certain times during the
Bananas are usually ripened to color stage 3 to 4 year, 20 to 30 days before harvest (Stover and
before delivery to distribution centers, retailers, or Simmonds 1987). The fruit is unacceptable for
wholesalers. market, although eating quality is unaffected. This
disorder has been associated with water deficits at
Within certain limits, the period required for bunch emergence during rapid fruit growth under
ripening bananas can be shortened or extended very humid and hot conditions (Daniells et al.
to meet trade requirements by adjusting the 1987, Williams et al. 1989, 1990). If bananas and
temperature. Under average conditions, depending plantains are exposed to temperatures above 30 to
on initial temperatures chosen and condition of 35 ºC, ripening can be irreversibly inhibited (high-
the fruit, the ripening cycle can be as short as 4 temperature injury).
days (above 18 ºC) or may be extended to 8 to 10
days (at 14 ºC). If initial ripening temperatures are
too high (above 25 ºC), the pulp will soften but Postharvest Pathology
the peel will remain green (a condition described
as “cooked” or “boiled” fruit) (Robinson 1996, The main postharvest diseases of bananas and
Turner 1997). Uneven ripening can be caused plantains are crown rot—a disease complex
by low temperatures and insufficient ethylene. caused by several fungi (Colletotrichum musae,
Ripening rates and characteristics of the fruit vary Fusarium semitectum, Fusarium pallidoroseum,
to some extent between lots depending on cultivar, Lasiodiplodia theobromae, Botryodiplodia
country of origin, weather conditions during the theobroame, Ceratocystis paradoxa, Verticillium
growing of the fruit, temperatures during handling sp., Acremonium sp., and Curvularia sp.)—and
and transit, and maturity of the fruit. Hard-green anthracnose (Colletotrichum musae) (Stover
fruit (less full) will take longer to ripen than more 1972, Wardlaw 1972, Stover and Simmonds 1987,
advanced and full fruit. Snowdon 1990, Jeger et al. 1995). Anthracnose
is a latent infection that occurs in the plantation.
Though it can appear on green fruit, it is more
Respiration Rates apparent in ripening fruit as numerous small,
dark, circular spots. Crown-rot organisms
Temperature mg CO2 kg-1 h-1 normally enter after harvest, usually as a result of
13 ºC 20 to 80 mechanical injury to the fruit.
15 ºC 26 to 140
18 ºC 32 to 200 Other diseases that from time to time become
20 ºC 40 to 280 important locally include stem-end rot
Data modified from Kader (1998). (Lasiodiplodia theobromae / Thielaviopsis
paradoxa), in which the invaded flesh becomes
227
brown, soft, and water-soaked, and cigar-end Acknowledgments
rot (Verticillium theobromae / Trachysphaera
fructigena), in which the rotted tip of the finger is Some of the information in this chapter was from
dry and appears similar to the ash of a cigar (Stover the University of California, Davis, website “Fresh
1972, Wardlaw 1972, Stover and Simmonds Produce Facts” at http://postharvest.ucdavis.edu/
1987, Snowdon 1990, Jeger et al. 1995). Sigatoka produce_information.
disease of bananas is caused by Mycosphaerella
sp. and has been reported in most banana/plantain-
producing countries (Snowdon 1990). The potential References
of this fungal disease is such that a flourishing
banana industry can be destroyed within a few Akkaravessapong, P. 1986. The ripening of
years. Fruit symptoms include premature ripening, bananas increases their susceptibility to mechanical
buff-colored pulp, and increased susceptibility to damage. M.Sc. (Agric) Prelim. Report, University
chilling injury. Preventive and control measures to of Western Australia, Perth, Australia. Unpublished
reduce decay incidence begin with strict sanitation report.
in plantation and packing plant, postharvest
treatment with systemic fungicides, minimizing Bishop, D. 1990. Controlled atmosphere storage.
mechanical damage during handling, prompt In C.J.V. Dellino, ed., Cold and Chilled Storage
cooling of fruit to lowest safe temperature, and Technology, pp. 66-98. Blackie, London, U.K.
expediting transport to final destination.
Daniells, J.W., B.J. Watson, P.J. Farrell, and
J.C. Mulder. 1987. Soil water stress at bunch
Quarantine Issues emergence increases maturity bronzing of banana
fruit. Queen. J. Agric. An. Sci. 44:97-100.
There are no quarantine issues.
Jeger, M.J., S. Eden-Green, J.M. Thresh, et al.
1995. Banana diseases. In S. Gowen, ed., Bananas
Suitability as Fresh-Cut Product and Plantains, pp. 317-381. Chapman and Hall,
London, U.K.
Bananas and plantains are not good candidates
for fresh-cut processing because of their very John, P., and J. Marchal. 1995. Ripening and
high oxidation and browning potential (John and biochemistry of the fruit. In S. Gowen, ed.,
Marchal 1995). Bananas and Plantains, pp. 434-467. Chapman and
Hall, London, U.K.
228
Kader, A.A. 1997. A summary of CA Stover, R.H. 1972. Banana, Plantain, and Abaca
recommendations for fruits other than apples Diseases. Commonwealth Agricultural Bureaux,
and pears. In A.A. Kader, ed., 7th International Slough, U.K.
Controlled Atmosphere Research Conference,
University of California, Davis, CA, July 13-18, Stover, R.H., and N.W. Simmonds. 1987. Bananas.
1997, vol. 32, pp. 1-34. University of California, Longman Scientific and Technical, Essex, U.K.
Davis, CA.
Thompson, A.K., and G.B. Seymour. 1982.
Kader, A.A. 1998. Bananas. In Fresh Produce Comparative effects of acethylene and ethylene gas
Facts. At http://postharvest.ucdavis.edu/produce_ on initiation of banana ripening. Ann. Appl. Biol.
information. 101:407-410.
Liu, F.W. 1976. Banana response to low Thompson, A.K., and O.J. Burden. 1995.
concentrations of ethylene. J. Amer. Soc. Hort. Sci. Harvesting and fruit care. In S. Gowen, ed.,
101:222-224. Bananas and Plantains, pp. 403-433. Chapman and
Hall, London, U.K.
Peacock, B.C. 1972. Role of ethylene in the
initiation of fruit ripening. Queensland J. Agric. Turner, D.W. 1997. Bananas and plantains. In S.K.
Animal Sci. 29:137-145. Mitra, ed., Postharvest Physiology and Storage of
Tropical and Subtropical Fruits, pp. 47-83. CAB
Robinson, J.C. 1996. Bananas and Plantains. CAB Intl., Wallingford, U.K.
Intl., Wallingford, U.K.
Wardlaw, C.W. 1972. Banana Diseases Including
Scott, K.J., W.B. McGlasson, and E.A. Roberts. Plantain and Abaca. Longman, London, U.K.
1970. Potassium permanganate as an ethylene
absorbent in polyethylene bags to delay ripening Wei, Y., and A.K. Thompson. 1993. Modified
of bananas during storage. Austr. J. Exp. Agric. atmosphere packaging of diploid bananas (Musa
Animal Husb. 10:237-240. AA). In J. de Baerdemaeker, B. McKenna, M.
Janssens, A. Thompson, F. Artes Calero, E.
Scott, K.J. 1977. Some Australian contributions Hohn, and Z. Somogyi, eds., Proceedings of the
on postharvest physiology and pathology of the COST-94 Workshop on Postharvest Treatment
banana fruit. Tech. Bull. 38, ASPAC Food and of Fruit and Vegetables: Systems and Operations
Fertilizer Technology Center,Taipei, Taiwan. for Postharvest Quality, September 14-15, 1993,
Leuven, Belgium, pp. 235-246. Commission of the
Simmonds, N.W., and K. Shepherd. 1955. The European Communities, Leuven, Belgium.
taxonomy and origins of the cultivated bananas. J.
Linnean Soc. (Botany) 55:302-312. Williams, M.H., M. Vesk, and M.G. Mullins.
1989. Characteristics of the surface of banana peel
Snowdon, A.L. 1990. Postharvest Disease and in cultivars susceptible and resistant to maturity
Disorders of Fruits and Vegetables, vol. 1, General bronzing. Can. J. Bot. 67:2154-2160.
Introduction and Fruits. CRC Press, Boca Raton,
FL. Williams, M.H., M. Vesk, and M.G. Mullins. 1990.
Development of the banana fruit and occurrence of
Sommer, N.F., and M.L. Arpaia. 1992. Postharvest the maturity bronzing disorder. Ann. Bot. 65:9-19.
handling systems: tropical fruits. In A.A. Kader,
ed., Postharvest Technology of Horticultural
Crops, 2nd ed., pp. 241-245. DANR Pub no. 3311,
University of California, Davis, CA.
229
Bean Grades, Sizes, and Packaging
Marita Cantwell U.S. grades for snap beans are U.S. Fancy,
U.S. No. 1, and U.S. No. 2, based primarily on
Cantwell is with the Department of Plant Sciences, external appearance. Though not sized for market,
University of California, Davis, CA. a reasonable size is expected according to the
characteristics of each variety. Beans are packed
in 11.4 to 13.6 kg (25 to 30 lb) crates and 6.8 or
Scientific Name and Introduction 9.1 kg (15 or 20 lb) cartons; presnipped beans for
foodservice are available in 4.5 kg (10 lb) bags.
Fresh snap or common beans (string beans, yellow
wax beans, green beans) (Phaseolus vulgaris L.),
runner or flat beans (P. coccineus L.), and long Cooling
beans (Vigna sesquipedalis) are members of the
Fabaceae (Leguminosae) family. Both the fleshy Snaps beans can be hydrocooled. This is
pod and seeds are consumed. Fresh pod beans especially beneficial in dry climates where
are available from many production areas in the dehydration is a concern and in situations where
United States, but in the winter months they are evaporation of surface moisture occurs rapidly
produced mainly in Florida and Mexico. after cooling (for example, beans packed in wire-
bound crates). Hydrocooling is very rapid, but
significant postharvest decay can occur if the
Quality Characteristics and Criteria product remains wet after cooling. Forced-air
cooling is the method of choice if beans have
Beans should be well-formed and straight, bright been packed. Efficient cooling is achieved without
in color with a fresh appearance free of defects, leaving free moisture on beans, but high relative
and tender (not tough or stringy) but firm. The humidity must be maintained to reduce excessive
diameter of the pod, rather than length, is a good water loss (Boyette et al. 1994, Sargent 1995).
indicator of quality. Buyers prefer pods with no or
only slight bulges indicating tender, young seeds.
As the name implies, snap beans should break Optimum Storage Conditions
easily when the pod is bent, giving off a distinct
audible snap. Poor quality is most often associated Snap beans should be stored at 5 to 7.5 °C (41
with overmaturity, broken beans, water loss, to 46 °F) with 95 to 100% RH. At 5 to 7.5 °C
chilling damage, and decay. (41 to 46 °F), a storage life of 8 to 12 days is
expected. Good quality can be maintained for
a few days at temperatures below 5 °C (41 °F),
Horticultural Maturity Indices but chilling injury will be induced (see Chilling
Sensitivity). Some chilling may occur even at the
Snap beans (yellow, green, and purple types) are recommended storage temperature of 5 °C (41 °F)
harvested when they are rapidly growing, about 8 after 7 to 8 days, depending on cultivar. Quality
to 10 days after flowering for typical mature snap rapidly decreases above 7.5 °C (46 °F) due to
beans. All pod beans should be harvested when the yellowing, seed development, and water loss.
pod is a bright color and fleshy, and the seeds are Waxed cartons and plastic film liners reduce water
small and green. After that period, excessive seed loss. The perishability and rates of water loss of
development reduces quality and the pod becomes immature beans are higher than for mature beans.
pithy and tough and loses its bright color. Long beans have postharvest requirements similar
to those of snap beans with similar responses to
chilling (Zong et al. 1992).
230
Controlled Atmosphere (CA) Freezing Injury
Considerations
Freeze damage occurs at -0.7 °C (30.7 °F) or
At recommended temperatures, 2 to 5% O2 below and appears as water-soaked areas that
reduces respiration (Saltveit 1997). Snap beans subsequently deteriorate and decay.
benefit from CO2 levels between 3 and 10% with
retention of color, reduced discoloration, and
reduced decay on damaged beans (Cano et al. Ethylene Production and Sensitivity
1997, Hardenburg et al. 1986, Trail et al. 1992).
Higher CO2 levels (20 to 30%) are used for short Beans produce only very low amounts of
periods (for example, 24 h) but can cause off ethylene (less than 0.05 µL kg-1 h-1 at 5 °C or 41
flavors (Costa et al. 1992). °F). Ethylene exposure greater than 0.1 µL L-1
promotes chlorophyll loss, increases browning,
and reduces green bean storage life by 30 to 50%
Retail Outlet Display Considerations at 5 °C (41 °F ) (Wills and Kim 1996).
231
Postharvest Pathology California, Davis, July 13-18, 1997, vol. 4, pp. 46-
52. University of California, Davis, CA.
Various decay organisms may attack fresh pod
beans as a result of chilling injury, surface Cantwell, M., and T. Suslow. 1998. Snap bean
moisture, or mechanical damage. Common produce facts. Recommendations for maintaining
decay-causing fungi are those causing “nesting postharvest quality. At http://postharvest.ucdavis.
decays” (cottony leak caused by Pythium spp. and edu/produce_information.
Rhizopus spp), gray mold (Botrytis cinerea), and
watery soft rot (Sclerotinia spp.). Water-soaked Costa, M.A.C., J.K. Brecht, S.A. Sargent, and D.J.
spots may be due to lesions caused by bacterial Huber. 1994. Tolerance of snap beans to elevated
infections (Pseudomonas spp. and Xanthomonas CO2 levels. Proceedings of the Annual Meeting of
spp.) (Snowdon 1992). the Florida State Horticultural Society 107:271-
273.
Beans are snipped to remove stems and tails for Saltveit, M.E. 1997. A summary of CA and MA
food service. Browning of the cut ends can be requirements and recommendations for harvested
a problem, and high-CO2 atmospheres retard vegetables. In M.E. Saltveit, ed., 7th International
discoloration. Controlled Atmosphere Research Conference,
University of California, Davis, July 13-18, 1997,
vol. 4, pp. 98-117. University of California, Davis,
Special Considerations CA.
Extra careful and expedited postharvest handling Sargent, S.A. 1995. Optimizing packing and
are required for highly perishable, very fine cooling methods for maintaining high quality snap
French beans (haricot verts) to avoid physical beans. In G.J. Hochmuth and D.N. Maynard, eds.,
damage and dehydration. FACTS Proceedings, p. 8. University of Florida,
Gainesville, FL.
233
Beet 0.5 kg (1.1 lb) and must contain at least 3 beets.
Fresh-market bunches are packed in small crates
Franciszek Adamicki of 10 to 15 kg (22 to 33 lb) capacity, whereas
beets intended for storage are packed in 20 kg (44
Adamicki is with the Department of Vegetable lb) polyethylene-lined crates or bins of 500 to 600
Storage, Research Institute of Vegetable Crops, kg (1,100 to 1,320 lb) capacity.
Skiermiewice, Poland.
Precooling Conditions
Scientific Name and Introduction
Bunched beets should be precooled to below 4 °C
Beta vulgaris L. Crassa group—table beet, or red (39 °F) within 4 to 6 h of harvest. Hydrocooling,
beet—is a biennial of the Chenopodiaceae family. forced-air cooling, and package icing are common
In the first year, it forms a fleshy storage root cooling methods. Proper precooling and packaging
(enlarged hypocotyl) that is edible. In the early retard subsequent discoloration of the leaves,
stages of plant development, the whole plant may weight loss, and decay. Mature harvested beets
be consumed. Beets are grown worldwide, the top should be precooled within 24 h after harvest to
producers being Germany, Poland, the Russian below 5 °C (41 °F) with forced-air cooling.
Federation, and the United States.
Optimum Storage Conditions
Quality Characteristics and Criteria
Bunched beets can be kept for about 10 to 14 days
Quality criteria include root shape, root size at 0 °C (32 °F) and above 98% RH. Topped beets
(diameter), color, firmness (turgidity), smoothness, should be stored at 1 to 2 °C (33 to 36 °F) and
cleanness, trimming of rootlets, and freedom 98% RH. During storage at 0 to 1 °C (32 to 34
from defects. Intensive and uniform color with °F), more black spot and rot occur than at higher
minimum zoning is the most important quality temperatures (Schouten and Schaik 1980). Red
criterion. beets can be in air-ventilated storage for 4 to 6
mo and in mechanical refrigerated storage for as
long as 8 to 10 mo. Before storage, beets should
Horticultural Maturity Indices be topped and sorted to remove all diseased or
mechanically damaged roots. Large roots keep
Fresh-market bunched beets (with tops) are much better than small ones because they lose
harvested as early as 50 to 70 days after planting; water and shrivel more slowly.
whereas roots (without tops) are usually harvested
later but before they reach full maturity, especially Red beets can be stored in pits and trenches,
when they are intended for long-term storage. especially where winter temperatures are low for
prolonged periods. Insulation of pits (clamps) and
trenches is needed to avoid injurious temperature
Grades, Sizes, and Packaging fluctuations. The temperature in pits (clamps) and
cellars should not drop below -0.5 °C (31 °F) or
Grades U.S. No. 1 and U.S. No. 2 are based exceed 5 °C (41 °F) to minimize losses caused by
primarily on external appearance. Unless freezing, sprouting, and rotting.
otherwise specified, the diameter of each beet
shall not be less than 2.5 to 3.8 cm (1 to 1.5 in).
Standard bunches shall be fairly uniform in size,
and each bunch of beets shall not weigh less than
234
Controlled Atmosphere (CA) Physiological Disorders
Considerations
Death of shoots and breakdown of the top part
There is little or no benefit from CA or MA of roots are common problems during long-term
storage of beet roots. Elevated levels of CO2 storage at 0 ºC (32 °F). Physiological disorders
(over 5%) in the atmosphere may promote decay can appear quickly during subsequent shelf-life at
(Shipway 1968). 20 ºC (68 °F) after storage (Adamicki and Badełek
1997).
Quarantine Issues
Ethylene Production and Sensitivity
None.
Beet roots produce very low amounts of ethylene,
(<0.1 µL kg-1 h-1) and are not particularly sensitive
to ethylene exposure. Suitability as Fresh-Cut Product
No current potential.
Respiration Rates
235
Hardenburg, R.E., A.E. Watada, and C.Y.
Wang. 1986. The Commercial Storage of Fruits,
Vegetables, and Florist and Nursery Stocks.
Agriculture Handbook 66, U.S. Department
of Agriculture, Agricultural Research Service,
Washington, DC.
236
Blackberry Horticultural Maturity Indices
237
Retail Outlet Display Conditions juice) (Perkins-Veazie et al. 1996, Mitcham et al.
1998).
Blackberries should be stored and displayed at the
coldest refrigeration temperature possible and with
no mist. As little as 1 day at room temperature can Postharvest Pathology
stimulate growth of gray mold.
The most common postharvest diseases are gray
mold (Botrytis cinerea) and rhizopus rot (Rhizopus
Chilling Sensitivity stolonifer) (Jennings 1988, Ellis et al. 1991).
No known issues.
Ethylene Production and Sensitivity
238
Controlled Atmosphere Research Conference,
University of California, Davis, July 13-18, 1997,
vol. 3, pp. 1-34. University of California, Davis,
CA.
239
Blueberry Quality Characteristics and Criteria
Blueberries (Vaccinium sp.) are a member of the Grades, Sizes, and Packaging
heath family (Ericaceae) grown as a perennial
crop. Blueberries have a blue to blue-black Highbush blueberries are graded as U.S. No. 1 or
epidermis or skin that is covered by a waxy are unclassified. Size can be used in connection
bloom, giving the fruit a light-blue appearance. with grade: extra large is fewer than 90 berries
The flesh is juicy and creamy white to green in per cup (250 mL), large is 90 to 129 berries per
color. Blueberries of commercial importance cup, medium is 130 to 189 berries per cup, and
include the lowbush (V. angustifolium Ait.), small is 190 to 250 berries per cup (AMS 1966).
northern (V. corymbosum L.), southern highbush Blueberries are packaged as 1- or 2-pint units,
(hybrids of V. corymbosum, V. ashei, and V. usually in polyethylene or polystyrene-ventilated
darrowi Camp.), and rabbiteye (V. ashei Reade). clamshells, and sold as 12-unit trays.
Lowbush blueberries are much smaller (less than
1 g) than highbush types and are prized for their
use in processed products such as pie and pastry Precooling Conditions
fillings, pancakes, muffins, jams, and sauces.
Northern highbush berries are grown primarily Blueberries for fresh market should be forced-
in the northern tier of the United States and air cooled to under 10 ºC (50 °F), graded, then
require 600 to 1,000 h of chilling below 7 °C maintained under refrigeration at 0 to 3 °C (32
(45 °F) (Pritts 1992). Southern highbush berries to 37 °F) within an hour after harvest to remove
require fewer chill hours and can lose fruit to field heat and extend storage life. Precooling to
frost damage if grown in areas where spring 5 ºC (41 °F) can cause condensation problems
temperatures drop below 0 °C (32 °F) during in lowbush blueberries when packed at ambient
bloom and early fruit set. Rabbiteye blueberries temperatures but should be incorporated when
are hardy only in the Southern United States or delays between picking and packing exceed 21 h
where winter temperatures remain above 10 °C (Jackson et al. 1999).
(50 °F). These berries often have a gritty mouth
feel from seeds and stone cells and lack a well-
developed calyx but have a longer shelf-life and Optimum Storage Conditions
more pigment than highbush berries.
Blueberries should be held at -0.5 to 0 ºC (31 to
32 ºF) and at above 90% RH for up to 2 weeks
for lowbush, northern highbush, and southern
highbush (Perkins-Veazie et al. 1995, Jackson et
al. 1999) and up to 4 weeks for rabbiteye (Miller
et al. 1988).
240
Controlled Atmosphere (CA) Physiological Disorders
Considerations
The major disorders are shrivel (water loss),
Rabbiteye, highbush, and lowbush blueberries sunscald, and fruit cracking.
benefit from 10 to 15% CO2 + 1 to 10% O2 when
held at or below 5 ºC (41 °F) (Smittle and Miller
1988, Prange et al. 1995). Firmness and total Postharvest Pathology
acidity were maintained and decay decreased with
a shelf-life up to 6 weeks. Blueberries are susceptible to Botrytis
cinerea (gray mold) and anthracnose (ripe rot,
Colletotrichum gloesosporiodes) (Milholland
Retail Outlet Display Considerations 1995). At temperatures above 10 ºC (50 °F),
Rhizopus stolonifer can grow readily in fruit
Blueberries should be stored and displayed under packs.
refrigeration with temperatures as close to 0 °C
(32 °F) as possible.
Quarantine Issues
Respiration Rates
Special Considerations
-1 -1
Temperature mg CO2 kg h
0 ºC 2 to 10 Bruising caused by improper handling or
4 to 5 ºC 9 to 12 mechanical harvesting reduces the storage life
10 ºC 23 to 35 of fresh fruit. Mechanical harvesting can reduce
15 to 16 ºC 34 to 62 fruit storage life by half compared with hand
20 to 21 ºC 52 to 87 harvesting.
25 to 27 ºC 78 to 124
241
Davis, website “Fresh Produce Facts” at http:// Pritts, M.P. 1992. Site selection and preparation.
postharvest.ucdavis.edu/produce_information. In M. Pritts and J. Hancock, eds., Highbush
Blueberry Production Guide. NRAES-55,
Cooperative Extension Service, Ithaca, NY.
References
Smittle, D.A., and W.R. Miller. 1988. Rabbiteye
AMS [USDA Agricultural Marketing Service]. blueberry storage-life and fruit quality in
1966. United States Standards for Grades of controlled atmospheres and air storage. J. Amer.
Blueberries. U.S. Government Printing Office, Soc. Hort. Sci. 113:723-728.
Washington, DC.
Suzuki, A., T. Kikuchi, and K. Aoba. 1997.
El-Agamy, S.Z.A., M.M. Aly, and R.H. Biggs. Changes of ethylene evolution, ACC content,
1982. Fruit maturity as related to ethylene in ethylene forming enzyme activity and respiration
‘Delite’ blueberry. Proceedings of the Annual in fruits of highbush blueberry. J. Japan. Soc.
Meeting of the Florida State Horticultural Society Hort. Sci. 66:23-27.
95:245-246.
242
Bok Choy Optimum Storage Conditions
Scientific Name and Introduction Low-O2 atmospheres of 0.5 to 1.5% retard leaf
yellowing caused by chlorophyll degradation
Bok choy (Brassica campestris L. ssp. chinensis) (O’Hare et al. 1995). A combination of 5% CO2
is also known as Chinese chard, boy-toyo, pak- + 3% O2 delays leaf yellowing and senescence
choy, and pak-tsoi (King 1989). Bok choy is a during storage (Wang and Herner 1989).
nonheading type of Chinese cabbage (Li 1981). It
is an annual in the Cruciferae family. The edible
portions are the shiny, dark green leaves and the Retail Outlet Display Considerations
thick, chalk-white stalks (Peirce 1987). Most U.S.
bok choy is produced in California. Bok choy is displayed loosely on refrigerated
shelves. Misting should be done to minimize
moisture loss and wilting.
Quality Characteristics and Maturity
Indices
Chilling Sensitivity
High-quality bok choy has thick, fleshy, firm
stalks and glossy, dark-green leaves. Bok choy Bok choy is not chilling sensitive and should be
with bruised or slimy spots and wilted leaves stored as cold as possible without freezing. It
should be avoided. freezes at -0.5 °C (31 °F).
Bok choy is mainly supplied to ethnic markets in Bok choy produces very small amounts of
the United States, but many large supermarkets ethylene, 0.1 to 0.2 µL kg-1 h-1, and is not overly
are carrying it in their “ethnic” fresh produce sensitive to ethylene.
departments. There are no standard U.S. grades.
Respiration Rates
Precooling Conditions
Temperature mg CO2 kg-1 h-1
Prompt precooling to near 0 °C (32 °F) is 0 °C 5 to 6
important to maintain freshness and for extended 5 °C 10 to 12
storage. 10 °C 19 to 21
15 °C 34 to 44
20 °C 48 to 63
Data from Luo and Zheng (2001).
243
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Peirce, L.C. 1987. Vegetables: Characteristics,
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and Production, and Marketing. John Wiley & Sons,
1.8 at 20 °C (68 °F). To calculate heat production, New York, NY.
multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal per tonne per day. Wang, H., and R.C. Herner. 1989. Effect of CA
storage on the ultrastructure of chloroplasts and
chlorophyll content of Chinese mustard. In E.
Physiological Disorders and Postharvest Kupferman, and M. Patterson, eds., Proceedings
Pathology of the 5th International Controlled Atmosphere
Research Conference, vol. 2, pp. 9-17. Wenatchee,
Leaf yellowing indicates that senescence has WA.
occurred during extended storage or storage at
higher than optimal storage temperatures. Storing
bok choy at 0 to 5 °C (32 to 41 °F) will mitigate
this problem.
Quarantine Issues
None.
No current potential.
References
244
Breadfruit drain from the fruit after harvest before washing in
water to avoid latex stain.
Robert E. Paull and Nancy Jung Chen
Paull and Chen are with the Department of Grades, Sizes, and Packaging
Tropical Plant and Soil Sciences, University of
Hawaii at Manoa, Honolulu, HI. There are no U.S. or international grade standards.
Fruit are graded according to size and various
counts per fiberboard carton containing 9 to 18
Scientific Name and Introduction kg (20 to 40 lb). Fruit are sold on a weight basis.
Telescoping two-piece fiberboard cartons are
The tropical breadfruit (Artocarpus altilis generally used for packaging. One-piece cartons
[Parkins] Fosb.) develops from the whole having dividers to minimize fruit movement and
inflorescence and is normally round, sometimes rubbing also are used.
cylindrical, 15 to 30 cm (6 to 12 in) in diameter,
and weighs from 0.5 to 3 kg (1.1 to 6.6 lb)
(Nakasone and Paull 1998). The fruit receptacle Precooling Conditions
(core) is surrounded by a pale yellow-white,
edible pulp that is covered by a yellowish green, Cool and ship fruit as soon as possible after
thin, reticulated skin. Most varieties are seedless harvest. Room cooling to 12 ºC (54 ºF) is
(Ragone 1991). Seeded varieties have from 10 generally used. Do not use hydrocooling as it
to 150, 2.5-cm-long (1-in-long) brown seeds leads to skin browning.
(Bennett and Nozzolillo 1987). The tropical
breadfruit is widely grown in tropical areas.
Optimum Storage Conditions
245
Chilling Sensitivity reported (Salunke and Desai 1984). Purseglove
(1968) reported a fruit rot caused by Rhizopus
Long-term storage is not possible. At 12 ºC (54 artocarpi in India.
ºF), chilling injury symptoms begin to develop
within 7 days (Marriott et al. 1979). Symptoms are
a brown, scaldlike discoloration of the skin, failure Quarantine Issues
to fully soften, poor flavor development, and an
increase in decay. Breadfruit is a fruit fly host and has been
successfully treated by vapor heat treatment and
irradiation.
Ethylene Production and Sensitivity
Special Considerations
Respiration Rates
Fruit can be boiled, dried, used in breadmaking,
Temperature mg CO2 kg h -1 -1 or fermented. Slices can be fried or stored in brine
13 ºC 94 to 564 (Whitney 1988, Bates et al. 1991). The sweet ripe
25 ºC 362 to 597 fruit is eaten as a dessert. The cooked seeds are
Data from Worrell and Carrington (1997) and also eaten.
Worrell et al. (1998).
Fruit rot due to Phytophthora palmivora and pink Purseglove, J.W. 1968. Tropical Crops.
disease (Botryobasidium salmonicola) have been Dicotyledons. Longman, London, U.K.
246
Ragone, D. 1991. Ethnobotany of breadfruit in
Polynesia. In P.A. Cox and S.A. Banack, eds.,
Islands, Plants, and Polynesians—An Introduction
to Polynesian Ethnobotany, pp. 203-220.
Dioscorides Press, Portland, OR.
247
Broccoli Grades, Sizes, and Packaging
Peter M.A. Toivonen and Charles Forney There are three grades: U.S. Fancy, U.S. No.
1, and U.S. No. 2. They are based on external
Toivenen is with Pacific Agri-Food Research appearance, level of damage, trimming, and stalk
Centre, Agriculture and Agri-Food Canada, diameter (AMS 1943). Two or three heads are
Summerland, British Columbia, Canada; Forney normally bunched together for the fresh market.
is with Atlantic Food and Horticultural Research Such bunches are packed 14 to 18 units in waxed
Centre, Agriculture and Agri-Food Canada, cardboard boxes and weigh approximately 10 kg
Kentville, Nova Scotia, Canada. (21 lb) (Boyette et al. 1996). Larger heads may
have the stem trimmed to produce “crowns,”
and these are packed loose in 9 kg (20 lb) boxes.
Scientific Name and Introduction Individual florets are also cut and packed in 2.5 to
5 kg (5.5 and 11 lb) film bags for hotel, restaurant,
Brassica oleracea L., Italica group, also known and institutional use.
as broccoli, calabrese, or sprouting broccoli, is a
native of southern Europe and a member of the
Brassicaceae (Cruciferae) family. The crop is Precooling Conditions
annual and grows to maturity in about 75 to 95
days, depending on cultivar, season, and planting Field-packed broccoli is commonly cooled by
date. The whole immature influorescence (head) is injecting liquid ice into waxed cartons (Cantwell
the edible portion, with the floret tissue most often and Suslow 1999). Ice maintains the proper
preferred by consumers. It grows best in cool temperature and RH for transport and distribution.
climates and is available year-round from many Hydrocooling and forced-air cooling are also
areas of North America, though a large portion is options, but good temperature management is
grown in California. required during transport (Cantwell and Suslow
1999).
248
Controlled Atmosphere (CA) Respiration Rates
Considerations
Temperature mg CO2 kg-1 h-1
The recommended CA for broccoli is 1 to 2% 0 °C 20 to 22
O2 and 5 to 10% CO2 at 0 to 5 °C (32 to 41 °F) 5 °C 32 to 36
(Makhlouf et al. 1989, Cantwell and Suslow 10 °C 76 to 86
1999). Optimal CA conditions can double storage 15 °C 160 to 180
life, especially when broccoli is held above 20 °C 280 to 320
optimum temperatures. However, if O2 drops to Data from Cantwell and Suslow (1999).
below 1% in storage, there is a risk of off odors
caused by the generation of sulfur-containing Respiration rates for cut florets are slightly higher
volatiles (Forney et al. 1991). High rates of air (Izumi et al. 1996).
exchange are recommended in shipping containers
to avoid accumulation of off odors. Modified To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
atmosphere packaging systems generally maintain by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
both O2 and CO2 at 10% to prevent accumulation 1.8 at 20 °C (68 °F). To calculate heat production,
of off odors. multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal per tonne per day.
Broccoli is commonly converted to fresh-cut floret Canaday, C.H., J.E. Wyatt, and J.A. Mullins. 1991.
products. Stems are also shredded into a packaged Resistance in broccoli to bacterial soft rot caused
coleslaw-type product. by Pseudomonas marginalis and fluorescent
Pseudomonas species. Plant Dis. 75:715-720.
AMS [USDA Agricultural Marketing Service]. Forney, C.F., J.P. Mattheis, and R.K. Austin.
1943. United States Standards for Grades of 1991. Volatile compounds produced by broccoli
Bunched Italian Sprouting Broccoli. At http:// under anaerobic conditions. J. Agric. Food Chem.
www.ams.usda.gov/AMSv1.0/standards. 39:2257-2259.
250
Gillies, S.L., and P.M.A. Toivonen. 1995. Cooling
method influences the postharvest quality of
broccoli. HortScience 30:313-315.
251
Brussels Sprouts decay, and damage (AMS 1954). To meet these
grades, sprouts should be larger than 2.5 cm (1
Charles Forney and Peter M.A. Toivonen in) in diameter and shorter than 7 cm (2.75 in).
Brussels sprouts are packaged loose in 11-kg
Forney is with Atlantic Food and Horticulture (25-lb) cartons or in 3.6- to 4.6-kg (8- to 10-lb)
Research Centre, Agriculture and Agri-Food flats or cartons containing 12 plastic containers
Canada, Kentville, Nova Scotia, Canada; of sprouts (Boyette et al. 1996). Plastic liners are
Toivonen is with Pacific Agri-Food Research often used in cartons with loose sprouts to reduce
Centre, Agriculture and Agri-Food Canada, moisture loss. In addition, polyethylene bags are
Summerland, British Columbia, Canada. sometimes used in place of the plastic containers
for consumer units.
252
4 weeks. CO2 ranging from 5 to 10% and O2 of 1 To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
to 2% are beneficial (Lyons and Rappaport 1962, by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
Isenburg 1969, Lipton and Mackey 1987). CO2 1.8 at 20 °C (68 °F). To calculate heat production,
above 10% and O2 below 0.5% can be injurious. multiply mg kg-1 h-1 by 220 to get BTU per ton per
CO2 above 20% causes internal browning and day or by 61 to get kcal per tonne per day.
pitting of outer leaves (Lyons and Rappaport
1962), while O2 at 0.5% or less can induce a
reddish-tan discoloration of the heart leaves and Physiological Disorders
bitter flavor (Lipton and Mackey 1987). Brussels
sprouts do not produce severe off odors when held Internal browning, or “tipburn,” is the margins of
in low O2 as do other Brassica vegetables such as inner leaves in buds turning brown and is caused
broccoli (Forney and Jordan 1999). by inadequate transport of calcium to young
expanding leaves. Growing conditions that favor
rapid growth promote internal browning (Maynard
Retail Outlet Display Considerations and Barker 1972).
Special Considerations
Respiration Rates
Brussels sprouts can be stored attached to their
Temperature mg CO2 kg-1 h-1 stem to prolong storage life. Packaging in vented
0 °C 20 to 60 poly-bags or overwrapped cups reduces wilting.
5 °C 44 to 96 Sprout freeze at -0.8 °C (30.6 °F) (Cantwell 2001).
10 °C 126 to 168
15 °C 128 to 272
20 °C 172 to 380
Data from Lyons and Rappaport (1959).
253
References Ryall, A.L., and W.J. Lipton. 1979. Handling,
Transportation and Storage of Fruits and
AMS [USDA Agricultural Marketing Service]. Vegetables, vol. 1, Vegetables and Melons. AVI
1954. United States Standards for Grades of Publishing Co., Westport, CT.
Brussels Sprouts. At http://www.ams.usda.gov/
AMSv1.0/standards. Snowdon, A.L. 1991. A Color Atlas of Postharvest
Diseases and Disorders of Fruits and Vegetables,
Boyette, M.D., D.C. Sanders, and G.A. Rutledge. vol. 2, Vegetables. Wolfe Publishing Ltd., London,
1996. Packaging requirements for fresh fruits and U.K.
vegetables. At http://www.bae.ncsu.edu/programs/
extension/publicat/postharv/ag-414-8/index.html. Stenvers, N. 1971. Preliminary experiments
on vacuum cooling of vegetables and berries.
Cantwell, M. 1997. Properties and recommended Sprenger Institute Report 1630 (Suppl.), Sprenger
conditions for storage of fresh fruits and Institute, Berlin, Germany.
vegetables. At http://postharvest.ucdavis.edu.
Stewart, J.K., and W.R. Barger. 1963. Effects of
Forney, C.F., and M.A. Jordan. 1999. Anaerobic cooling method, prepacking and top-icing on the
production of methanethiol and other compounds quality of brussels sprouts. Proc. Amer. Soc. Hort.
by Brassica vegetables. HortScience 34:696-699. Sci. 83:488-494.
254
Cabbage Horticultural Maturity Indices
255
Optimum Storage Conditions 10% CO2 may injure stored cabbage. Low-O2 and
high-CO2 injury is slow to appear and the extent
Cabbage should be stored at 0 °C (32 °F) with of the injury depends on the cultivar and maturity
98 to 100% RH. Storage at -1 °C (31 °F) may (Masters and Hicks 1990). For example, low-O2
cause freezing, while storage at 1 °C (34 °F) injury does not occur in oxheart cabbage until
may promote senescence-related storage losses, after 35 days when held in 0% O2 (100% N2) at
especially if held in long-term storage—for 0 to 4 °C (32 to 39 °F) (Schouten et al. 1997),
example, 6 mo (R. Prange, unpublished data). and green cabbage does not show low-O2 injury
High RH minimizes decay and trimming losses until after 2, 3, or 6 mo, if held at 0 °C (32 °F)
(van den Berg 1987). The presence of light in in 0.5, 1.0 or 1.5% O2, respectively (Masters and
the storage room reduces physiological disorders Hicks 1990, Menniti et al. 1997). Similarly, CO2
such as leaf yellowing and weight loss (Prange injury is seen after 2 mo if held at 0 °C (32 °F)
and Lidster 1991). Cabbage is stored in bins or in 20% CO2 or after 2.5 mo (Menniti et al. 1997)
in bulk (IOS 1991). Only three to six wrapper or 6 mo (Masters and Hicks 1990) in 10% CO2.
leaves should be left on the head (Hardenberg Symptoms of low-O2 and high-CO2 injury are off
et al. 1986). All loose leaves should be trimmed flavors and off odors as well as visible damage
before storage because they will interfere with (Lougheed 1987, Ludford and Isenberg 1987,
air circulation between heads. Air circulation Masters and Hicks 1990, Menniti et al. 1997,
in the storage should be sufficient to maintain Schouten et al. 1997). Both low O2 and high CO2
constant and uniform temperature and RH around produce very similar visible damage beginning
all cabbage heads. Bulk-stored cabbage should in the meristematic tissue located at the apex of
be ventilated in a vertical direction and the depth the stem in the middle of the cabbage. Damage
should not exceed 3 m (9.8 ft). Bin-stored cabbage spreads to outer leaves and appears as black spots
should be arranged to maximize uniform air (low O2) (Schouten et al. 1997) or bronzing (high
flow around each bin. Storage life depends on CO2) (Masters and Hicks 1990). Lougheed (1987)
cultivar (for example, early-maturing cultivars suggested there may be no interaction between
tend to have shorter storage life than late-maturing low O2 and high CO2 in injury, but Kaji et al.
cultivars), quality (for example, freedom from (1993) showed that high CO2 (5 to 15%) keeps
decay), and storage conditions (Pritchard and shredded cabbage in good condition if O2 is high
Becker 1989, IOS 1991, Boyette et al. 2008a). (5 to 10%).
The end of storage life is signaled by increased
respiration rate, core elongation, and sometimes
rootlet development on the core butt (Guffy and Retail Outlet Display Considerations
Hicks 1985, Pritchard and Becker 1989).
Damaged outer wrapper leaves should be
trimmed. Trimming may expose lighter green
Controlled Atmosphere (CA) inner leaves, but natural or artificial light can
Considerations increase the chlorophyll content and green color
(Perrin 1982). The greatest concern is loss of
Cabbage is the most common vegetable stored moisture, which can be prevented by wrapping
under CA (Saltveit 1997, Thompson 1998). Wide each head in a clear plastic film, frequent water
ranges of O2 levels from 1.5 to 5% and CO2 levels sprinkling, and/or displaying in a refrigerated
from 0 to 8% have been recommended. Therefore, cabinet.
the mid range of 2 to 3% O2 and 4 to 5% CO2 is
probably a good general recommendation. Low O2
reduces color and trimming loss and inhibits root Chilling Sensitivity
growth, while elevated CO2 reduces rot, decay,
and sprouting (Saltveit 1997). Atmospheres with Cabbage is not chilling sensitive. The freezing
lower than 1.5 to 2% O2 and/or higher than 8 to point is -0.9 to -0.83 °C (30.4 to 30.5 °F)
256
(Hardenburg et al. 1986, Pritchard and Becker multiply mg kg-1 h-1 by 220 to get BTU per ton per
1989). Even though cabbage with core day or by 61 to get kcal per tonne per day.
temperature of -1.1 °C (30 °F) before harvest can
show no evidence of freeze damage (Pritchard and
Becker 1989), storage at -1.0 °C (30.2 °F) is not Physiological Disorders
advisable because it can produce freeze damage,
especially on outer leaves (R. Prange, unpublished The physiological cause is unknown for some
data). Temperature oscillations during cycling disorders of stored cabbage (for example, black
of mechanical refrigeration may expose tissue to midrib, black speck of cabbage [pepper spot,
freezing temperatures if the setpoint is too low or spotted necrosis], gray speck, and necrotic spot)
if the hystersis is too large. (Bérard 1994). The occurrence of these disorders
is influenced by cultivar and cultural practices,
especially mineral nutrition. Some storage
Ethylene Production and Sensitivity disorders are clearly frost-induced (for example,
black blotching, black spot, epidermal detachment,
Cabbage produces very little ethylene (<0.1 μL frost blemishing, and redheart). Bérard (1994) also
kg-1 h-1 at 20 °C [68 °F]) (Kader 1992). Kubo et al. describes storage disorders caused by dormancy,
(1990) detected only a trace amount of ethylene ethylene, and head maturity.
from cabbage at 25 °C (77 °F). When heads are
stored in the dark at 5 °C (41 °F) in sealed plastic
bags, ethylene reaches only 1 μL L-1, regardless of Postharvest Pathology
cultivar (Meinl and Bleiss 1986).
The major cause of postharvest decay in cabbages
Cabbage should not be exposed to ethylene after is the gray mold fungus (Botrytis cinerea)
harvest. Ethylene increases respiration (Inaba (Geeson 1983, Snowden 1991). Gray mold can
et al. 1989), and concentrations as low as 1 μL be minimized by using resistant cultivars, using
L-1 accelerate senescence and quality loss (for preharvest fungicides, practicing strict hygiene,
example, leaf yellowing, wilting, and abscission avoiding mechanical or frost damage, rapid cooling
more in air than in CA) (Hicks and Ludford 1980, to 0 °C (32 °F), and using CA storage (Snowden
Pritchard and Becker 1989). Reduced ethylene 1991). Alternaria rot, also known as dark, black,
production and phenylalanine ammonia-lyase or gray leaf spot and caused by Alternaria spp.,
(PAL) activation is linked to less tissue browning infects a wide range of cruciferous vegetables
in shredded cabbage (Takahashi et al. 1996) and can cause significant storage losses (Geeson
1983, Snowden 1991, Cerkauskas 1994). Since this
disease is commonly transmitted through infected
Respiration Rates seed, it can be minimized by using disease-free
seed, rotation with noncruciferous crops, applying
Temperature mg CO2 kg-1 h-1 preharvest fungicides, destruction of diseased
0 °C 4 to 6 material before storage, and using rapid cooling to
4 to 5 °C 9 to 12 0 °C (32 °F). There are other fungi (such as ring
10 °C 17 to 19 spot), bacteria (bacterial rots and watery soft rot,
15 to 16 °C 20 to 32 for example), and a virus (tobacco mosaic virus)
20 to 21 °C 28 to 49 that cause significant losses (Dennis 1983, Ceponis
25 to 27 °C 49 to 63 et al. 1987, Snowden 1991).
Data from Hardenburg et al. (1986).
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Quarantine Issues
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production, None.
257
Suitability as Fresh-Cut Product
Shredded cabbage is suitable as a fresh-cut product, packaged in air or MAP. Gorny (1997) indicates MA
treatment efficacy as good in extending storage life of shredded cabbage and provides respiration rates at
different temperatures, atmospheres, and varying amounts of shredding.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and 1.8 at
20 °C (68 °F). To calculate heat production, multiply mg kg-1 h-1 by 220 to get BTU per ton per day or by
61 to get kcal per tonne per day. Data from Gorny (1997).
258
Cantwell, M., and T. Suslow 2008. Cabbages Hicks, J.R., and P.M. Ludford. 1981. Effects of
(round and Chinese types): recommendations low ethylene levels on storage of cabbage. Acta
for maintaining postharvest quality. (http:// Hort. 116:65-73.
postharvest.ucdavis.edu/produce_information)
Inaba, A., Y. Kubo, and R. Nakamura. 1989. Effect
Ceponis, M.J., R.A. Cappellini, and G.W. of exogenous ethylene on respiration in fruits and
Lightner. 1987. Disorders in cabbage, bunched vegetables with special reference to temperature.
broccoli and cauliflower shipments to the New J. Jap. Soc. Hort. Sci. 58:713-718.
York market, 1972-1985. Plant. Dis. 71:1151-
1154. IOS [International Organization for
Standardization]. 1991. Round-headed cabbage—
Cerkauskas, R.F. 1994. Alternaria diseases. In R.J. guide to cold storage and refrigerated transport.
Howard, J.A. Garland, and W.L. Seaman, eds., Intl. Std. Ref. No. ISO 2167:1991(E), Geneva,
Diseases and Pests of Vegetable Crops in Canada, Switzerland.
pp. 96-97. Canadian Phytopathological Society
and Entomological Society of Canada, Ottawa, Kader, A.A. 1992. Postharvest biology and
Canada. technology: an overview. In A.A. Kader, ed.,
Postharvest Technology of Horticultural Crops,
Geeson, J.D. 1983. Brassica. In C. Dennis, ed., pp. 15-20. DANR Pub. no. 3311, University of
Postharvest Pathology of Fruits and Vegetables, California, Davis, CA.
pp. 125-126. Academic Press, New York, NY.
Kaji, H., M. Ueno, and Y. Osajima. 1993. Storage
Ghosh, S.P., and D.L. Madhavi. 1998. Cabbage. In of shredded cabbage under a dynamically
D.K. Salunkhe and S.S. Kadam, eds., Handbook controlled atmosphere of high O2 and high CO2.
of Vegetable Science and Technology, pp. 299- Biosci. Biotech. Biochem. 57:1049-1052.
321. Marcel Dekker, New York, NY.
Kubo, Y., A. Inaba, and R. Nakamura.
Gorny, J.R. 1997. A summary of CA and MA 1990. Respiration and C2H4 production in
requirements and recommendations for fresh- various harvested crops held in CO2-enriched
cut (minimally processed) fruits and vegetables. atmospheres. J. Amer. Soc. Hort. Sci. 115:975-
In J.R. Gorny, ed., 7th International Controlled 978.
Atmosphere Research Conference, University of
California, Davis, July 13-18, 1997, vol. 5, pp. 30- Lougheed, E.C. 1987. Interactions of oxygen,
66. University of California, Davis, CA. carbon dioxide, temperature, and ethylene that
may induce injuries in vegetables. HortScience
Guffy, S.K., and J.R. Hicks. 1985. Effect of 22:791-794.
cultivar, maturity and storage on respiration, dry
weight and glucosinolate content of cabbage. Acta Ludford, P.M., and F.M.R. Isenberg. 1987.
Hort. 157:211-218. Brassica crops. In J. Weichmann, ed., Postharvest
Physiology of Vegetables, pp. 497-522. Marcel
Hardenburg, R.E., A.E. Watada, and C.Y Wang. Dekker, New York, NY.
1986. The Commercial Storage of Fruits,
Vegetables, and Florist and Nursery Stocks. Masters, J.F., and J.R. Hicks. 1990. Cabbage. In
Agriculture Handbook 66, U.S. Department P.D. Lidster, G.D. Blanpied, and R.K. Prange,
of Agriculture, Agricultural Research Service, eds., Controlled-Atmosphere Disorders of
Washington, DC. Commercial Fruits and Vegetables, pp. 29-30.
1847/E, Agriculture Canada, Ottawa, Canada.
259
Meinl, G., and W. Bleiss. 1986. Beobactungen zur Snowden, A.L. 1991. A Colour Atlas of
Ethylenbildung und zur seiner Wirkung wahrend Postharvest Diseases and Disorders of Fruits and
der lagerung von Kopfkohl (Brassica oleracea Vegetables, vol. 2, Vegetables. Wolfe Publishing,
L. var. capitata) (Observations on ethylene London, U.K.
synthesis and its effects during storage of cabbage
[Brassica oleracea L. var. capitata]). Archiv für Takahashi, T., K. Abe, and K. Chachin. 1996.
Zuchtungforschung 16:267-270. Studies on the physiological and chemical changes
in shredded cabbage. III. Effect of air-exposure at
Menniti, A.M., M. Maccaferi, and A. Folchi. 1997. low temperature on physiological activities and
Physio-pathological responses of cabbage stored browning of shredded cabbage. J. Jap. Soc. Hort.
under controlled atmospheres. Postharv. Biol. Sci. 43:663-667.
Technol 10:207-212.
Thompson, A.K. 1998. Controlled Atmosphere
NASS [USDA National Agricultural Statistics Storage of Fruits and Vegetables. CAB Intl.,
Service]. 2005. Agricultural Statistics. At http:// Wallingford, U.K.
www.nass.usda.gov/index.
van den Berg, L. 1987. Water vapor pressure. In
Perrin, P.W. 1982. Poststorage effect of light, J. Weichmann, ed., Postharvest Physiology of
temperature and nutrient spray treatments on Vegetables, pp. 203-230. Marcel Dekker, New
chlorophyll development in cabbage. Can. J. Plant York, NY.
Sci. 62:1023-1026.
260
Carambola harvested at the color break stage (O’Hare
1993). Fruit that are 50 to 75% yellow are firmer
Robert E. Paull and Ching Cheng Chen than full-color fruit, and thus are regarded as
commercially mature. Fruit continue to develop
Paull and Chen are with the Department of color after harvest, though there is little other
Tropical Plant and Soil Sciences, University of change in quality.
Hawaii at Manoa, Honolulu, HI.
Grades, Sizes, and Packaging
Scientific Name and Introduction
There are no U.S. or international grades.
The carambola (Averrhoa carambola L.) is Carambola fruit are sold in 3.5-kg (7-lb) flats, 10-
a star-shaped fruit that has a waxy skin with kg (22-lb) single layers, 9-kg (20-lb) suitcases,
several smooth brown seeds. The flesh and skin and clam shells (16 fruit in 1 layer or 32 fruit in
are crisp and juicy (Nakasone and Paull 1998). 2 layers). Fruit require careful packing to reduce
The carambola is also referred to as “star fruit.” damage. A plastic or foam sleeve or wax paper
However, the name is not preferred, because there should be used.
is another tropical fruit called star fruit. Carambola
is grown widely in the tropics and in the warmer
areas of the subtropics. Precooling Conditions
261
Retail Outlet Display Considerations Respiration rate and pattern depend on cultivar
and maturity at harvest (Shiesh et al. 1987).
Do not display green fruit and do not stack more
than two or three fruit high to avoid mechanical
injury to the fragile wings (ribs). Misting is Physiological Disorders
acceptable.
The major problem is physical injury, especially
on the rib edges, that leads to browning. Injury
Chilling Sensitivity due to abrasion and impact can be avoided by
careful handling. Browning due to mechanical
During low-temperature storage at 0 ºC (32 ºF) injury can intensify with water loss. Fruit that
and 5 ºC (41 ºF) for 2 and 6 weeks, respectively, have lost about 5% of their weight due to water
some small surface pitting and rib-edge browning loss show visible symptoms of dehydration.
occurred, with severity of injury increasing with
storage time (Wan and Lam 1984). Greener fruit
are more susceptible to chilling injury (Wan Postharvest Pathology
and Lam 1984, Kenney and Hull 1986). Surface
pitting and rib-edge browning can also be seen Anthracnose (Colletotrichum gloeosporioides)
with desiccation, which may not be true chilling is most common, and the symptoms are thin,
injury. light-brown patches on fruit edges (Watson
et al. 1988). Diseases caused by Alternaria
alternata, Cladosporium cladosporioides, and
Ethylene Production and Sensitivity Botryodiplodia theobroma have been reported.
These diseases mainly occur at physical injury
These nonclimacteric fruit have a low production sites with prolonged storage.
rate, under 3 µl C2H4 kg-1 h-1 at 20 ºC (68 ºF),
depending on maturity (Oslund and Davenport
1983). Ethylene treatment (100 µL L-1 for 24 h) Quarantine Issues
slightly hastens degreening but has little effect
on flavor. Higher rates of ethylene production Carambola is a fruit fly host. Irradiation and cold
have been recorded after 12 days at 20 ºC (68 ºF) treatment (14 days at 1 ºC) are recommended.
(Shiesh et al. 1987) and may be associated with
decay.
Suitability as Fresh-Cut Product
262
Kader, A.A. 1999. Carambola (star fruit). Produce Wan, C.K., and P.F. Lam. 1984. Biochemical
Facts. Perishables Handling Quarterly, no. 93, pp. changes, use of polyethylene bags and chilling
19-20. University of California, Davis, CA. injury of carambola (Averrhoa carambola L.)
stored at various temperatures. Pertanika 7:39-46.
Kenney, P., and L. Hull. 1986. Effect of storage
condition on carambola quality. Proceedings of the Watson, B.J., A.P. George, R.J. Nissen, and B.I.
Annual Meeting of the Florida State Horticultural Brown. 1988. Carambola a star on the horizon.
Society 99:222-224. Queensland Agric. J. 114:45-51.
263
Carrot Grades, Sizes, and Packaging
Yaguang Luo, Trevor Suslow, and Marita Cantwell Carrots can be harvested either bunched or top-
trimmed; top-trimmed is the dominant method.
Luo is with the Food Quality Laboratory, Henry A. The common grades for bunched carrots are U.S.
Wallace Beltsville Agricultural Research Center, No. 1 and U.S. Commercial. For topped carrots,
Agricultural Research Service, U.S. Department of the grades are U.S. Extra No. 1, U.S. No. 1, U.S.
Agriculture, Beltsville, MD; Suslow and Cantwell No. 1 Jumbo, and U.S. No. 2. Topped carrots are
are with the Department of Plant Sciences, Mann typically packed in 0.5- to 2.25-kg (1- to 5-lb)
Laboratory, University of California, Davis, CA. perforated plastic “Cello-pack” bags that are
grouped in 11- or 22- to 22.7-kg (24- or 48- to 50-
lb) cartons or master poly bags. Bunched carrots
Scientific Name and Introduction are packed loosely in 12-kg (26-lb) cartons.
Quality criteria vary with use. High-quality carrots Storage at 0 to 1 °C (32 to 34 °F) is essential to
are firm, straight from “shoulder” to “tip,” smooth minimize decay and sprouting during storage.
with little residual “hairiness,” sweet with no High RH is required to prevent desiccation and
bitter or harsh taste, and show no signs of cracking loss of crispness. Mature topped carrots can be
or sprouting (Suslow and Cantwell 1998). stored for 7 to 9 mo at 0 °C (32 °F) with 98 to
100% RH. However, commercial storage and
distribution conditions rarely achieve the optimum
Horticultural Maturity Indices storage conditions and topped carrots are often
stored for 5 to 6 mo at 0 to 5 °C (32 to 41 °F)
Harvest maturity varies with the market outlet and with 90 to 95% RH. Common “Cello-pack”
use. Fresh market carrots are harvested partially carrots are typically immature and may be stored
mature, when the roots are about 1.8 cm (0.75 in) successfully for 2 to 3 weeks at 3 to 5 °C (37 to 41
or larger in diameter at the upper end (Kotecha et °F). Bunched carrots are highly perishable due to
al. 1998). Late harvesting may improve storability the presence of leaves and can be maintained for
by reducing decay during extended storage only 8 to 12 days. Bunched carrots are typically
(Suojara 1999). Fresh-cut processing carrots are shipped and stored with shaved or flaked ice.
harvested immature to ensure they are tender and
sweet.
264
Controlled Atmosphere (CA) Respiration Rates
Considerations
Temperature Topped Bunched
CA does not extend storage life of carrots beyond -1 -1
--------------mg CO2 kg h -----------------
that in air with high RH (Leshuk and Saltveit 0 °C 10 to 20 18 to 35
1990). Low O2 (1%) inhibited sprouting but also 5 °C 13 to 26 25 to 51
promoted decay (Abdel-Rahman and Isenberg 10 °C 20 to 42 32 to 62
1974). CO2 injury appears as soft brown spots 15 °C 26 to 54 55 to 106
upon exposure to air. CO2 levels above 5% 20 °C 46 to 95 87 to 121
promote decay. Storage at O2 levels below 3% can Data from Hardenburg et al. (1986).
result in increased bacterial rot, off flavors, and off
odors (Leshuk and Saltveit 1990). To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
Retail Outlet Display Considerations multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal per tonne per day.
Carrots are often displayed loosely on a shelf
with mist or in perforated plastic “Cello-pack”
consumer packages. Physiological Disorders
265
Sclerotinia, but tissue damage and color loss occur Hardenburg, R.E., A.E. Watada, and C.Y.
after treatment (Liew and Prange 1994). Good Wang. 1986. The Commercial Storage of Fruits,
sanitation during packing and storage at 0 °C (32 Vegetables, and Florist and Nursery Stocks.
°F) minimizes postharvest diseases. Agriculture Handbook 66. U.S. Department
of Agriculture, Agricultural Research Service,
Washington, DC.
Quarantine Issues
Kotecha, P.M., B.B. Desai, and D.L. Madhavi.
None 1998. Carrot. In D.K. Salunke and S.S. Kadam,
eds., Handbook of Vegetable Science and
Technology, pp.119-139. Marcel Dekker, New
Suitability as Fresh-Cut Product York, NY.
266
Suojara, T. 1999. Effect of harvest time on the
storage performance of carrot. J. Hort. Sci.
Biotech. 74:484-492.
267
Cassava white to light yellow. Principal causes for loss are
vascular streaking and decay. Extended storage
Steven A. Sargent can have two adverse effects on quality: starches
are converted to sugars, and roots become fibrous,
Sargent is with the Horticultural Sciences lengthening cooking time (Booth et al. 1976).
Department, University of Florida, Gainesville,
FL.
Horticultural Maturity Indices
Scientific Name and Introduction Harvest maturity is based on the root size desired
by the market and ranges from 6 to 18 mo after
Cassava (Manihot esculenta Crantz) is a woody the stem sections are planted. Sweet types usually
perennial shrub of the Euphorbiaceae family and is grow faster than bitter types. The main stem
native to the Amazon region and Central America. is often trimmed to approximately 1 m (39 in)
It is widely grown throughout the tropics for its in height a few days before harvest. Plants are
starchy root (Rubatsky and Yamaguchi 1997). manually pulled, or the root zone mechanically
Cassava is called “yuca” in Spanish, “mandioca” undercut to facilitate plant removal, and individual
in Portuguese, “cassave” in Haitian Creole, and roots are cut. Cassava roots are turgid at harvest
“manioc” in French. It is consumed in a variety and must be handled carefully to avoid splitting
of ways, but only after some form of processing. the periderm. Harvest may be delayed until
Cultivars are classified into two groups based on market, processing, or other conditions are
the amounts of hydrogen cyanide present. Sweet favorable, since cassava roots can be stored in the
types contain less than 50 mg kg-1 HCN (fresh ground for up to 24 mo.
weight) and are generally sold as fresh roots,
whereas bitter types have higher amounts of HCN
along with higher yields and starch content (da Grades, Sizes, and Packaging
Conceição 1980). These latter types are processed
into products including flour (from coarse to finely There are no U.S. grade standards for cassava.
textured), tapioca starch, and fermented starch. In However, shippers should consult with buyers
2002, 184 million tonnes of cassava was produced to define quality expectations. For example,
worldwide; major producers were Africa (Nigeria) root lengths in excess of 30 cm (11.8 in) are
(99 million tonnes), Asia (52 million tonnes), undesirable to many importers. Commercially,
and Latin America and the Caribbean (32 million roots are cleaned by brushing, rinsing in water,
tonnes). Cassava is adapted to semiarid climates surface-drying, and coating with paraffin wax
and has been a traditional crop for subsistence prior to packing in corrugated cartons.
farmers, though it is increasingly cultivated as an
agronomic crop.
Precooling Conditions
268
1976). Paraffin wax is applied to roots exported Respiration Rates
to the United States. Waxing and holding at 0
to 5 ºC (32 to 41 °F) extends shipping time to After 1 day of storage in air at 25 °C (77 °F) with
over 30 days with minimal vascular streaking. A 98% RH, unwaxed roots respired at a rate of 23
water-based carnauba wax maintained postharvest μL CO2 kg-1 h-1, whereas those treated with 75 µL
quality equivalent to paraffin wax (Sargent et al. L-1 ethylene respired at a rate of 32 μL kg-1 h-1.
1995). After 4 days under the same conditions, internal
CO2 production was 8 and 11 μL kg-1 h-1, while O2
levels were 9 and 11.5 μL kg-1 h-1 for unwaxed and
Controlled Atmosphere (CA) waxed roots, respectively (Aracena 1993).
Considerations
269
Quarantine Issues Snowdon, A.L. 1992. A Color Atlas of Postharvest
Diseases and Disorders of Fruits and Vegetables,
There are no restrictions on imports from the vol. 2, Vegetables. CRC Press, Boca Raton, FL.
major production areas.
Wheatley, C.C., and W.W. Schwabe. 1985.
Scopoletin involvement in postharvest
Suitability as Fresh-Cut Product physiological deterioration of cassava root
(Manihot esculenta Crantz). J. Expt. Bot.
There is potential for peeled intact or sliced roots, 36:783‑791.
but shelf-life is currently limited to 2 or 3 days
under ideal storage conditions due to vascular
streaking.
References
270
Cauliflower Grades, Sizes, and Packaging
Charles Forney and Peter M.A. Toivonen There are two grades, U.S. No. 1 and U.S.
Commercial. Grading is based on size, external
Forney is with Atlantic Food and Horticulture appearance, decay, damage, and trimming (AMS
Research Centre, Agriculture and Agri-Food 1968). Heads are normally well-trimmed and
Canada, Kentville, Nova Scotia, Canada; packed in a single layer with 12 to 24 heads in
Toivonen is with Pacific Agri-Food Research a carton weighing 11.4 to 13.6 kg (25 to 30 lb).
Centre, Agriculture and Agri-Food Canada, Cauliflower may also be packed in 27-kg (60-
Summerland, British Columbia, Canada. lb) wire-bound crates or 23-kg (50-lb) cartons/
crates (Boyette et al. 1996). Cauliflower is
commonly packed in the field, where leaves
Scientific Name and Introduction are trimmed closely to the head and heads are
overwrapped with a perforated plastic film. The
Brassica oleracea L., Botrytis group of the overwrap should have four to six 6-mm (0.25-in)
Brassicaceae (Cruciferae) family, is also known holes to allow adequate ventilation but minimize
as cauliflower. It is derived from the wild cabbage dehydration. Individual florets are also cut and
that is native to southern Europe. Cauliflower packed in 2.3- and 4.6-kg (5- and 10-lb) film bags
produces an edible head of malformed and for institutional use, or smaller units for consumer
condensed flowers (the curd) whose stalks are packs.
short, fleshy, and closely crowded. Cauliflower is
sensitive to its growing environment and requires
cool temperatures, plenty of moisture, and good Precooling Conditions
fertility to produce quality heads. Therefore,
much of commercial production is in coastal areas Cauliflower is mostly vacuum-cooled or
having moderate temperatures, or the crop is hydrocooled prior to storage or marketing.
grown to take advantage of the cooler times of the Vacuum cooling works well for field-packed heads
growing season. and is more effective if cauliflower is wetted
before cooling (Stewart and Barger 1961). Forced-
air cooling can also be used.
Quality Characteristics and Criteria
271
Controlled Atmosphere (CA) To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
Considerations by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
The benefits from CA and modified atmosphere multiply mg kg-1 h-1 by 220 to get BTU per ton per
are modest (Saltveit 1997). Low O2 (below 2%) day or by 61 to get kcal per tonne per day.
in combination with 3 to 5% CO2 may delay leaf
yellowing and the onset of curd browning (Romo-
Parada et al. 1989). However, injury may occur Physiological Disorders
at below 2% O2 and/or above 5% CO2 (Lipton
et al. 1967, Lipton and Harris 1976). Injury may Black speck is a disorder in which necrotic lesions
not be apparent until curds are cooked, when they 0.5 to 4 mm (0.02 to 0.16 in) in diameter appear
become soft, grayish, and develop off flavors. on the surface of branches or flower stalks in
Storage at CO2 levels above 10% can induce injury the interior of the curds. This disorder is more
within 2 days. Unlike broccoli, fresh cauliflower prevalent on certain cultivars and is most severe
does not produce strong off odors when held in in cauliflower produced during warm weather and
low-O2 atmospheres (Forney and Jordan 1999). rapid growth (Loughton and Riekels 1988).
Cauliflower has a very low ethylene production Heads are susceptible to freezing injury, which
rate, <1 μL kg-1 h-1 at 20 ºC (68 °F). It is extremely appears as water-soaked and grayish curds, if held
sensitive to ethylene, with the most prevalent below 0.8 °C (30.6 °F).
symptoms being curd discoloration and leaf
yellowing and abscission.
Postharvest Pathology
Respiration Rates
The major causes of postharvest decay are
Temperature mg CO2 kg h -1 -1 bacterial soft rot caused by Erwinia and
0 °C 16 to 18 Pseudomonas spp. and brown rot caused by
5 °C 20 to 22 Alternaria spp. (Ceponis et al. 1987). Storing only
10 °C 32 to 36 good quality, disease-free heads and maintaining
15 °C 42 to 50 good temperature control can best control these
20 °C 74 to 84 decay organisms.
25 °C 86 to 98
Data from Suslow and Cantwell (2008).
272
Quarantine Issues Dearborn, C.H., and G.J. Raleigh. 1935. A
preliminary note on the control of internal
None. browning of cauliflower by the use of boron. Proc.
Amer. Soc. Hort. Sci. 33:622-623.
Suitability as Fresh-Cut Product Forney, C.F., and M.A. Jordan. 1999. Anaerobic
production of methanethiol and other compounds
Cauliflower is commonly converted to fresh-cut by Brassica vegetables. HortScience 34(4):696-
floret products. 699.
Growing conditions can strongly influence the Leshuk, J.A., and M.E. Saltveit. 1990.
quality of fresh cauliflower. Heads must be Controlled atmosphere storage requirements and
protected from the sun, normally by tying leaves recommendations for vegetables. In M. Calderon
subtending the head together, to prevent yellowing and R. Barkai-Golan, eds., Food Preservation by
and strong flavor development in the curd. Only Modified Atmospheres, pp. 315-352. CRC Press,
high-quality heads should be stored or shipped Boca Raton, FL.
long distances. Heads must be handled gently to
avoid bruising, which results in rapid browning Lipton, W.J., and C.M. Harris. 1976. Response of
and decay (Suslow and Cantwell 2008). stored cauliflower (Brassica oleracea L., Botrytis
group) to low-O2 atmospheres. J. Amer. Soc. Hort.
Sci. 101:208-211.
Acknowledgments
Lipton W.J., C.M. Harris, and H.M. Couey. 1967.
Some of the information in this chapter was Culinary quality of cauliflower stored in CO2-
obtained from the Oregon State University enriched atmospheres. Proc. Amer. Soc. Hort. Sci.
website for “Commercial Vegetable Production 91:852-859.
Guides” at http://horticulture.oregonstate.edu/
content/vegetable-production-guides. Loughton, A., and J.W. Riekels. 1988. Black speck
in cauliflower. Can. J. Plant Sci. 68:291-294.
273
vol. 4, pp. 98-117. University of California, Davis,
CA.
274
Celeriac Precooling Conditions
Common packaging is 9.1-kg (20-lb) cartons, Celeriac is not chilling sensitive and should be
though 15.9-kg (35-lb) cartons are also used. stored as cold as possible without freezing.
There are no defined grading categories for
celeriac; it is graded and packed based on relative
size. Ethylene Production and Sensitivity
275
not be stored with other fruit or vegetables that References
produce high levels of ethylene.
Cantwell, M. 2001. Celeriac. At http://postharvest.
ucdavis.edu.
Respiration Rates
Kurki, L. 1971. Moisture in vegetable storage.
-1 -1
Temperature mg CO2 kg h Acta Hort. 20:146-151.
0 °C 5 to 8
5 °C 11 to 15 Morris, S. 2001. Fruit and vegetables storage and
10 °C 18 to 28 transport database. At http://www.postharvest.
15 °C 32 to 38 com.au/celeriac.pdf.
20 °C 41 to 49
Data from Morris (2001). Weichmann, J. 1976. Storage of celeriac. Indus.
Obst. Gemuseverwert. 61(8):205-207.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and Weichman, J. 1977. CA storage of celeriac. Acta
1.8 at 20 °C (68 °F). To calculate heat production, Hort. 62:109-118.
multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal per tonne per day. Wiersma, O. 1973. Storage of celeriac.
Betrijfsouteikkeling 4:79-80.
Physiological Disorders
Postharvest Pathology
Acknowledgments
276
Celery Grades, Sizes, and Packaging
Yaguang Luo, Trevor Suslow, and Marita Cantwell U.S. grades are U.S. Extra No. 1, U.S. No. 1, and
U.S. No. 2. Celery may be sold as “unclassified”
Luo is with the Food Quality Laboratory, Henry A. to designate a lot that has not been graded
Wallace Beltsville Agricultural Research Center, according to U.S. standards. In California, fresh-
Agricultural Research Service, U.S. Department of market celery is field-packed in 27.2-kg (60-lb)
Agriculture, Beltsville, MD; Suslow and Cantwell cartons containing 48 stalks or in 12.7-kg (28-lb)
are with Mann Laboratory, Department of Plant cartons containing 12 or 18 hearts. Florida celery
Sciences, University of California, Davis, CA. is packed in seven size grades from 18 to 96 stalks
per crate. Celery hearts are prepared from smaller
stalks that are trimmed to 20, 25, or 30 cm (8, 10,
Scientific Name and Introduction or 12 in) in length, and packed in 8- or 13-kg (18-
or 28-lb) cartons (Peirce 1987).
Celery (Apium graveolens L.) is a biennial of the
Umbelliferae (Apiaceae) family but is planted
and harvested as an annual crop. The edible Precooling Conditions
portion is the long, thick, green fleshy petioles
and, if present after trimming, associated leaves. Celery is typically hydrocooled or vacuum-cooled
California supplies 75% of U.S. production, with with a chilled water spray application. Prompt
other significant production coming from Florida precooling to near 0 °C (32 °F) is essential to
and Michigan (Schaffer 2000). maintain freshness and crispness, as well as for
extended storage.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate The most prominent storage decay is bacteria
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and soft rot (primarily caused by Pectobacterium or
1.8 at 20 °C (68 °F). To calculate heat production, Pseudomonas), gray mold (Botrytis cinerea), and
multiply mg kg-1 h-1 by 220 to get BTU per ton per watery soft rot (Sclerotinia spp.) (Snowden 1992).
day or by 61 to get kcal per tonne per day. Storage at 0 °C (32 °F) is important to minimize
losses due to postharvest decay. Controlled
atmospheres (1.5% O2 and 7.5% CO2) suppress
Physiological Disorders the growth of Sclerotinia and watery soft rot (Reys
and Smith 1986, Reys and Smith 1987). However,
Pithiness is a major source of quality loss and careful maintenance of atmospheric composition
decreased shelf-life (Saltveit and Mangrich is required as celery is susceptible to low-O2 and
high-CO2 injury.
278
Quarantine Issues Reyes, A.A., and R.B. Smith. 1987. Effect of
oxygen, carbon dioxide, and carbon monoxide on
None. However, export loads of celery may be celery in storage. HortScience 22:270-271.
fumigated at entry ports if common insects (aphids
and thrips) are found. Robbs, P.G., J.A. Bartz, G. McFie, and N.C.
Hodge. 1996. Causes of decay of fresh-cut celery.
J. Food Sci. 61:444-448.
Suitability as Fresh-Cut Product
Saltveit, M.E. 1997. A summary of CA and MA
The majority of fresh-cut celery is in the form of requirements and recommendations for harvested
celery sticks (cut petioles). Fresh-cut celery can vegetables. In M.E. Saltveit, ed., 7th International
be packed alone or in combination with other Controlled Atmosphere Research Conference,
vegetables, such as carrots and broccoli. The University of California, Davis, July 13-18, 1997,
shelf-life of fresh-cut celery is typically 12 to vol. 4, pp. 98-117. University of California, Davis,
14 days at 0 to 5 °C (32 to 41 °F). Discoloration CA.
of vascular tissue, splitting of the cut ends, and
bacteria decay are major problems limiting shelf- Saltveit, M.E., and M.E. Mangrich. 1996. Using
life of fresh-cut celery (Robbs et al. 1996, Saltveit density measurement to study the effect of
and Mangrich 1996). excision, storage, abscisic acid, and ethylene on
pithiness in celery petioles. J. Amer. Soc. Hort.
Sci. 121:137-141.
References
Shaffer, E., ed. 2000. Produce Availability
Gariepy, Y., G.S.V. Raghavan, R. Plasse, et al. and Merchandising Guide. The Packer. Vance
1984. Long-term storage of cabbage, celery, and Publishing Corp., Lenexa, KS.
leeks under controlled atmosphere. Acta Hort.
157:193-201. Snowdon, A.L. 1992. A Color Atlas of Postharvest
Diseases and Disorders of Fruits and Vegetables,
Hardenburg, R.E., A.E. Watada, and C.Y. vol. 2, Vegetables, pp. 182-195. CRC Press, Boca
Wang. 1986. The Commercial Storage of Fruits, Raton, FL.
Vegetables, and Florist and Nursery Stocks.
Agriculture Handbook 66, U.S. Department Suslow, T., and M. Cantwell. 2002. Celery
of Agriculture, Agricultural Research Service, Produce Facts. Recommendations for Maintaining
Washington, DC. Postharvest Quality. At http://postharvest.ucdavis.
edu/produce_information.
Leshuk, J.A., and M.E. Saltveit. 1990.
Controlled atmosphere storage requirements and Whiteman, T. 1957. Freezing Points of Fruits,
recommendations for vegetables. In M. Calderon Vegetables and Florist Stocks. Marketing Research
and R. Barkai-Golan, eds., Food Preservation by Report 196, U.S. Department of Agriculture,
Modified Atmospheres, pp. 315-352. CRC Press, Agricultural Marketing Service, Washington, DC.
Boca Raton FL.
Robert E. Paull and Nancy Jung Chen Fruit should be precooled as soon as possible after
harvest to about 12 ºC to 15 ºC (54 to 59 ºF), with
Paull and Chen are with the Department of room cooling or forced-air cooling used most
Tropical Plant and Soil Sciences, University of often.
Hawaii at Manoa, Honolulu, HI.
Optimum Storage Conditions
Scientific Name and Introduction
Store fruit at 10 to 13 ºC (50 to 55 ºF) with 90 to
The cherimoya (Annona cherimola Mill.) is a 95% RH for 2 to 3 weeks. If held at 20 ºC (68 ºF),
heart-shaped fruit having few seeds and a smooth fruit will last 3 to 4 days (Kader and Arpaia 1999).
skin that does not break apart during ripening. It is Storage is limited by skin darkening, desiccation,
grown in Florida, California, and Hawaii. and disease due to chilling injury. Ripe, soft fruit
should be held at 0 to 5 ºC (32 to 41 ºF).
280
Ethylene Production and Sensitivity sanitation. Careful handling and sanitation with
cooling, along with fungicides if approved, can
The cherimoya is a climacteric fruit and has minimize the problems.
high rates of ethylene production (100 to 300 µL
kg-1 h-1 at 20 ºC [68 ºF]) (Palma et al. 1993b).
Exposure to ethylene at 100 µL L-1 for 24 h leads Quarantine Issues
to rapid ripening of mature green fruit.
The cherimoya is a fruit fly host. Other quarantine
pests include seed borers and scales. Heat
Respiration Rates treatments and irradiation are potential treatments.
Premium sweet cherries have a bright, shiny Recommended conditions for storage of sweet
appearance. Fruit color can be dark red (‘Bing’), cherries are -1 to 0 ºC with RH at over 95%.
red (‘Sweetheart’), or yellow with a red blush Sweet cherries maintain good quality for 2 to 4
(‘Rainier’ and ‘Royal Ann’). The appearance of weeks under these conditions.
the stem, which should be green and free from
brown discoloration, is also critical for marketing.
Flavor is enhanced by highly soluble solids and Controlled Atmosphere (CA)
titratable acid content with a firm, juicy fruit Considerations
texture.
Reduction in the amounts of color change
(darkening), acid and firmness loss, incidence
Horticultural Maturity Indices of decay, and stem browning are potential
benefits of CA storage and MAP (modified
Fruit color is the most consistent and reliable atmosphere packaging). The effectiveness of these
maturity index. Different cultivars can be technologies is determined in part by fruit quality
harvested at slightly different color stages. For at harvest. Fruit harvested at a more advanced
example, ‘Bing’ cherries should be a mahogany stage of maturity (low acid, dark color, low
red color rather than lighter red (immature) or firmness) will not realize as much benefit from CA
282
or MAP. Optimal atmosphere conditions for CA 1.8 at 20 °C (68 °F). To calculate heat production,
range from 1 to 5% O2 with 5 to 20% CO2 (Chen multiply mg kg-1 h-1 by 220 to get BTU per ton per
1981, Patterson 1982, Mattheis et al. 1997). For day or by 61 to get kcal per tonne per day.
MAP, 5 to 10% O2 with 5 to 15% CO2 is effective
when fruit temperature is maintained at 0 to 5 ºC
(Mattheis and Reed 1994, Meheriuk et al. 1995). Physiological Disorders
Temperature control for MAP systems is critical
because the risk of anaerobiosis increases as Pitting and bruising are common problems caused
packaged fruit temperature increases. by harvest injury and rough postharvest handling
(Facteau and Rowe 1979, Thompson et al. 1997).
Fruit pitting is a manifestation of subsurface
Retail Outlet Display Considerations damage that develops into sunken areas near the
fruit surface. Bruising can occur from excess
Refrigeration during display is critical to reduce compression, drops or large impacts during
quality loss due to stem browning, shrivel, and harvest, transport, or packing. Visual symptoms of
development of decay. Fruit should be held at 5 pits and bruises often do not appear until well after
ºC or less to slow deterioration. Fruit should be the fruit have been packed, resulting in visible
refrigerated but not wetted because continuous damage appearing in wholesale or retail markets.
moisture on the surface can cause splitting.
Sweet cherries are also prone to shrivel and water
loss due to the lack of a well-developed cuticle.
Chilling Sensitivity Water loss can be minimized by prompt cooling
and storage in a high-RH environment.
Sweet cherries are not sensitive to chilling and
should be stored as cold as possible without Stem browning is another potential physiological
freezing. disorder. Stem browning can be minimized
by proper temperature and RH management;
however, packing procedures that scrape or injure
Ethylene Production and Sensitivity stems create wounds that will brown. In addition
to proper temperature management, use of
Sweet cherries produce very low amounts of chlorine dioxide in hydrocooler water can reduce
ethylene but will respond to exogenous or wound- development of stem browning (Roberts 1989).
induced ethylene with increased respiration and
quality loss.
Postharvest Pathology
283
decay (Willet et al. 1989). Low-temperature Chen, P.M., W.M. Mellenthin, S.B. Kelly, and
storage, fungicide application, and MAP with high T.J. Facteau. 1981. Effects of low oxygen and
CO2 (5 to 20%) all slow pathogen growth (English temperature on quality retention of ‘Bing’ cherries
and Gerhardt 1942, Gerhardt et al. 1942, 1956, during prolonged storage. J. Amer. Soc. Hort. Sci.
DeVries-Patterson et al. 1991, Brash et al. 1992, 106:533-535.
Spotts et al. 1998).
Crisosto, C.H. 1991. Sweet cherry harvesting,
postharvest handling and storage. Perishables
Quarantine Issues Handling Quarterly, no. 71. University of
California, Davis, CA.
Fruit exported to Japan must be fumigated with
methyl bromide to control codling moth larvae. DeVries-Patterson, R.M., A.L. Jones, and A.C.
Fruit shipped into California must be inspected Cameron. 1991. Fungistatic effects of carbon
and certified free from cherry fruit fly infestation. dioxide in a package environment on the decay of
Michigan sweet cherries by Monilinia fructicola.
Plant Dis. 75:943-946.
Suitability as Fresh-Cut Product
Do, J.Y., D.K. Salunkhe, D.V. Sisson, and A.A.
No current potential. Boe. 1966. Effects of hydrocooling, chemical,
and packaging treatments on refrigerated life and
quality of sweet cherries. Food Technol. 20:115-
Special Considerations 118.
Sweet cherries must be cooled promptly after Dugan, F.M., and R.G. Roberts. 1994. Etiology
harvest and low temperatures maintained of preharvest colonization of Bing cherry fruit by
throughout packing, storage, and transport. fungi. Phytopathology 84:1031-1036.
Low temperatures minimize quality loss as well
as physiological and pathological disorders. Dugan, F.M., and R.G. Roberts. 1997. Preharvest
Maintenance of low temperature is critical when fungal colonization affects storage-life of Bing
using MAP systems to avoid anaerobic conditions Cherry Fruit. J. Phytopathology 145:225-230.
and off-flavor development.
English, H., and F. Gerhardt. 1942. Effect of
carbon dioxide and temperature on the decay of
References sweet cherries under simulated transit conditions.
Proc. Am. Soc. Hort. Sci. 40:172-176.
Adaskaveg, J.E., and J.M. Ogawa. 1994.
Penetration of iprodione into mesocarp fruit tissue Facteau, T.J., and K.E. Rowe. 1979. Factors
and suppression of gray mold and brown rot of associated with surface pitting of sweet cherry. J.
sweet cherries. Plant Dis. 78:293-296. Amer. Soc. Hort. Sci 10:706-710.
Brash, D.W., L.H. Chea, and W. Hunt. 1992. Gerhardt, F., H. English, and E. Smith. 1942.
Controlled atmospheres inhibit storage rot of Respiration, internal atmosphere and moisture
cherries. In A.J. Popay, ed., Proceedings of the studies of sweet cherries during storage. Proc.
45th New Zealand Plant Protection Conference, Amer. Soc. Hort. Sci. 41:119-123.
August 11-13, 1992, pp. 136-137. New Zealand
Plant Protection Society, Auckland, New Zealand.
284
Gerhardt, F., H. Schomer, and T.R. Wright. Thompson, J.F., J.A. Grant, E.M. Kupferman, and
1956. Sealed Film Lug Liners for Packing Bing J. Knutson. 1997. Reducing sweet cherry damage
Cherries. Pub. no. 121, U.S. Department of in postharvest operations. HortTechnology 7:134-
Agriculture, Agricultural Marketing Service, 138.
Washington, DC.
Willett, M., G. Kupferman, R. Roberts, et al.
Mattheis, J.P., and N. Reed. 1994. Response of 1989. Integrated management of postharvest
‘Bing’ sweet cherries to modified atmosphere diseases and disorders of apple, pears and
packaging. Proceedings of the Oregon cherries. Washington State University Postharvest
Horticultural Society, January 1994, pp. 22-28. Pomology Newsletter 7:1-15.
285
Chicory (Belgian Endive or Witloof be sound, free of reddish blemish, frost damage,
Chicory) or traces of bruises, disease, insects, parasites,
or rodent attack. A chicon should feel heavy for
its size. Leaf tips should not curl back, and the
Vince Rubatzky and Mikal E. Saltveit base should be well-trimmed perpendicular to the
upright axis and without discoloration. It should
Rubatzky and Saltveit are with the Department of be lanceolate in shape, with the length ranging
Plant Sciences, University of California, Davis, from 2 to 3 times the maximum width. Quality is
CA. decreased by flower stem development in excess
of three-fourths of the bud’s length.
Scientific Name and Introduction Development of green leaf color is a quality
defect. Though leaf greening constitutes
Belgium endive or witloof chicory (Cichorium quality loss for many markets, U.S. consumers
intybus L.) is a biennial herbaceous plant tolerate a slight amount without significant
belonging to the Asteraceae family. In addition penalty. A small number of cultivars produce
to chicory’s use a leafy salad vegetable, the roots anthocyanin and are reddish-purple in coloration.
of some cultivars are grown for use as a coffee Elevated temperatures during holding and retail
substitute. Related vegetables of commercial value presentation are more important than exposure to
include lettuce (Lactuca sativa L., Cichorium light in inducing greening.
tribe), endive and escarole (Cichorium endivia
L.), and radicchio (Cichorium intybus L.). The
edible portion of chicory is the young, enlarged, Horticultural Maturity Indices
compact, and etiolated terminal bud that is
composed of young leaves and the partially Depending on temperature, optimum harvest is
suppressed but enlarging floral stem. In the trade, 20 to 30 days after forcing. Harvest delays result
this product is called a chicon. This large bud in elongated chicon and loss of compaction.
results from the forced growth of the apical bud Chicons are harvested when outer leaves are
on a defoliated and vernalized root in storage. The tightly appressed and density is maximal. The
roots are harvested after a year of growth, partially basal portions of the outermost leaves should
defoliated except for the apical bud, and stored be well sheathed. Leaf margins should be thin
until ready for forcing. and smooth. Timely harvest maximizes potential
shelf-life compared to harvesting at a later stage
Belgium endive is a popular vegetable in of development. Deterioration is mainly due to
northern European countries, where it is available marginal leaf drying or browning, which can occur
year-round. The crop is mostly for European rapidly (Herregods 1971).
consumption and of little interest elsewhere. In the
United States, it is not well known or appreciated
by the general public. Accordingly, production is Grades, Sizes, and Packaging
minor and supply is augmented by air shipments
from Europe. Grading is largely determined by uniformity of
shape, overall appearance, and the ratio of length
to width. Highest quality chicons are 9 to 17 cm
(3.5 to 6.8 in) in length and have a maximum
Quality Characteristics and Criteria
diameter of 6 cm (2.5 in). The typical ratio of
length to width is 2:1 to 3:1.
High-quality chicons are compact and turgid with
closely overlapping outer leaves having a mother-
U.S. grades are Extra, Standard, and Baby,
of-pearl luster or milky-white appearance and a
while European standards are Extra, Class
light yellow tinge on leaf margins. They should
286
I, II, and II irregular. Extra category chicons 0 °C (32 °F), even in the presence of light, but
are uniformly shaped, meet appropriate size greening increases with increasing temperature.
dimensions, have outer leaves that measure at
least half of the chicon length, are firm, and do not
exhibit greening or a glassy appearance. Lesser Controlled Atmosphere (CA)
quality involves less uniformity, less favorable Considerations
appearance, deterioration, and loss of compaction.
Storage life can almost be doubled by storage in
3 to 4% O2 with 4 to 5% CO2 at 0 °C (32 °F). CA
Precooling Conditions storage delays greening of leaf tips in light and
leaf spreading.
Trays holding the forced roots can be moved to a
cold room for a day or overnight before snapping
(that is, removal of chicons from the roots). Retail Outlet Display Considerations
Condensation on the chicons when transferred to a
warmer packing area is undesirable. Precooling in Maintain cold conditions to maximize storage and
this manner wastes energy because the entire roots shelf-life and minimize dehydration, but do not
of both marketable and unmarketable chicons are sprinkle or otherwise wet the product. Packaged
cooled. Hydrocooling is very effective, but water chicons should be held in conditions similar to
infiltration is difficult to remove and reduces those of packaged mushrooms, not in wet storage
shelf-life. Vacuum cooling is seldom used since areas.
it is expensive and results in a loss of moisture
accounting for 2 to 3% of the product’s fresh
weight. Forced-air cooling is occasionally used, Chilling Sensitivity
but it is not effective in cooling product in film
packages. Conventional room cooling is the most Chicory is not chilling sensitive, but it freezes at
commonly used method. It is the least expensive -0.5 °C (31 °F).
but is relatively slow compared with other
precooling techniques.
Ethylene Production and Sensitivity
In the United States, chicons are packed in
pasteboard containers that contain 4.5 kg (10 lb) Ethylene production is very low, but exposure to
of product. Perforated plastic film bags are also ethylene can produce physiological disorders such
used for packaging, as are film-overwrapped trays. as russet spotting and accelerated senescence.
Perforations account for about 0.5% of the bags
surface and are intended to limit condensation
because moisture on the chicons is detrimental to Respiration Rates
maintaining quality. Bags are opaque or covered
with translucent blue or green paraffin paper to Temperature mg CO2 kg-1 h-1
exclude light. 0 °C 2 to 3
5 °C 5 to 6
10 °C 12 to 14
Optimum Storage Conditions 15 °C 20 to 22
20 °C 35 to 38
Chicons should be stored in the dark at 0 °C (32 Data from Saltveit (2002, unpublished).
°F) and 95 to 100% RH (Hardenburg et al. 1986).
Storage life is reduced to 2 to 4 weeks at 2 ºC (36 To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
°F), 1 to 2 weeks At 5 ºC (41 °F), and 1 week or by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
less at 15 ºC (59 °F). There is little greening at 1.8 at 20 °C (68 °F). To calculate heat production,
287
multiply mg kg-1 h-1 by 220 to get BTU per ton per Special Considerations
day or by 61 to get kcal per tonne per day.
Chicons must be handled with care to avoid
mechanical damage to minimize discoloration
Physiological Disorders and pathological problems. Temperatures must be
kept low, and light must be excluded to prevent
Disorders include brown or hollow core, greening. High RH is necessary to prevent loss of
blackheart, foliage pinking, and red discoloration turgor and wilting.
of tissue that has been bruised or cut. A similar
reddish-orange coloration occurs when leaves split
or are torn. Additional disorders include russet References
spotting, the formation of light hairlike growth
on leaves, and leaf greening. Other causes of Corey, K.A., D.J. Marchant, and L.F. Whitney.
chicon deterioration are continued growth of the 1990. Witloof chicory: a new vegetable crop in the
stem, resulting in leaf spreading and opening; leaf United States. In J. Janick and J.E. Simon, eds.,
greening, loss of turgor, and wilting that results Advances in New Crops, pp. 414-418. Timber
in a loss of weight, grade, and quality; and the Press, Portland, OR.
appearance of bruises at the base or on the leaves
that become more apparent at retail. Chicons De Proft, M., J. De Greef, K. Van Nerum, and
showing any signs of cuts, drying, burses, or torn G. Goffings. 1986. Ethylene in the production of
tissue should be excluded from sale. Belgian endive. HortScience 21:1132-1133.
288
Chinese Cabbage Grades, Sizes, and Packaging
289
exposure to 7.5% CO2, or 5 to 10 days exposure Respiration Rates
to 30 to 40% (Herner 1987). Specific CA
recommendations depend on cultivar, temperature, Temperature mg CO2 kg-1 h-1
and storage duration. CA storage retains green 0 °C 6 to 14
color and ascorbate and sugar content in leaves 5 °C 8 to 16
and decreases decay (Wang 1983, Ludford and 10 °C 15 to 19
Isenberg 1987). 15 °C 19 to 30
20 °C 25 to 45
Data from Apeland (1985) and Cantwell and
Retail Outlet Display Considerations Suslow (2001).
Chinese cabbage is displayed as individual heads To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
with the outer leaves removed, frequently with a by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
band around the equator to maintain a compressed 1.8 at 20 °C (68 °F). To calculate heat production,
head shape. Heads should be kept as cool as multiply mg kg-1 h-1 by 220 to get BTU per ton per
possible without freezing and sprinkled with water day or by 61 to get kcal per tonne per day.
to minimize moisture loss. Exposure to ethylene
should be avoided.
Physiological Disorders
290
Suitability as Fresh-Cut Product is not essential for shredded Chinese cabbage held
at 0 or 5 °C (32 or 41 °F), as there is only a tran-
Chinese cabbage is suitable as a fresh-cut product sient increase in ethylene production and respiration
packaged in air or modified atmosphere packaging that peaks after 6 to 12 h and 0 to 3 h, respectively.
(MAP). Gorny (1997) rates MAP treatment as The shelf-life-limiting factors are browning on cut
moderately effective in extending the storage surfaces and leaf surfaces, as well as appearance of
life of shredded Chinese cabbage and provides black speck (gomasho). If 1% citrate is used as a
respiration rates at different temperatures, dip, a commercially acceptable shelf-life of 21 days
atmospheres, and varying amounts of shredding at 0 °C (32 °F) or 14 days at 5 °C (41 °F) can be
(table 1). Kleiber and Kim (1998) state that MAP achieved without MA.
5 °C 5 % O2 half head 10
+ 5 % CO2 rough cut (0.5 x 3 cm) 22
fine cut (0.25 - 1.5 cm) 28
291
References
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Apeland, J. 1985. Chilling injury in Chinese Postharvest handling systems: flower, leafy and
cabbage Brassica campestris pekinensis (Lour) stem vegetables. In A.A. Kader, ed., Postharvest
Olsson. Acta Hort. 157:261-270. Technology of Horticultural Crops, pp. 267-270.
DANR Pub. no. 3311, University of California,
Adamicki, F. 1997. Effect of ultra low oxygen Davis, CA.
on the storage and quality of some vegetables. In
M.E. Saltveit, ed., 7th International Controlled Kelley, William T. 1999. Specialty crops: chinese
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California, Davis, July 13-18, 1997, vol. 4, pp. 26- vegetable production. University of Georgia
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Cantwell, M., and T. Suslow. 2001. Cabbages pubcd/c809-w.htm#chinese_cabbage.
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464:249-251.
Fritz, D., and J. Weichmann. 1981. Influence of
weather conditions during growth on storage Ludford, P.M., and F.M.R. Isenberg. 1987.
ability of different Chinese cabbage cultivars. Brassica crops. In J. Weichmann, ed., Postharvest
Chinese Cabbage. In R.L. Villareal and T.D. Physiology of Vegetables, pp. 497-522. Marcel
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pp. 271-278. of Canada. National Research Council, Ottawa,
Ontario, Canada.
Geeson, J.D. 1983. Brassicas. In C. Dennis, ed.,
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pp. 125-126. Academic Press, New York, NY. and recommendations for the controlled and
modified atmosphere storage of harvested
Gorny, J.R. 1997. A summary of CA and MA vegetables. In M.E. Saltveit, ed., 7th International
requirements and recommendations for fresh- Controlled Atmosphere Research Conference, vol.
cut (minimally processed) fruits and vegetables. 4, Vegetables and Ornamentals, July 13-18, 1997,
In J.R. Gorny, ed., 7th International Controlled University of California, Davis, CA.
Atmosphere Research Conference, University of
California, Davis, July 13-18, 1997, vol. 5, pp. 30- Sozzi, A., F.L. Gorini, and L. Uncini. 1981.
66. University of California, Davis, CA. Storage suitability of the Chinese cabbage as
affected by lining. Acta Hort. 116:157-162.
Herner, R.C. 1987. High CO2 effects on plant
organs. In J. Weichmann, ed., Postharvest van den Berg, L. 1987. Water vapor pressure. In
Physiology of Vegetables, pp. 239-253. Marcel J. Weichmann, ed., Postharvest Physiology of
Dekker, New York, NY. Vegetables, pp. 203-230. Marcel Dekker, New
York, NY.
Kader, A.A. 1992. Postharvest biology and
technology: an overview. In A.A. Kader, ed., Wang, C.Y. 1983. Postharevst response of Chinese
Postharvest Technology of Horticultural Crops, cabbage to high CO2 treatment or low O2 storage.
pp. 15-20. DANR Pub. no. 3311, University of J. Amer. Soc. Hort. Sci. 108:125-129.
California, Davis, CA.
292
Wang, S.L., and R.F. Cerkauskas. 1999. Recent
development of production and processing of
oriental vegetables in Canada. Acta Hort. 483:429-
436.
293
Coconut Horticultural Maturity Indices
Robert E. Paull and Saichol Ketsa Young coconuts are harvested 6 to 9 mo after
flowering as they approach full size and the skin
Paull is with the Department of Tropical Plant and is still green (Consignado et al. 1976, Srivichai
Soil Sciences, University of Hawaii at Manoa, 1997) and the short stem (rachillae) on the top of
Honolulu, HI; Ketsa is with the Department of individual coconuts that originally held the male
Horticulture, Kasetsart University, Bangkok, flowers (“rat-tail” in Thai) becomes half green
Thailand. and brown. In immature nuts, the skin surface
around the calyx (cap) on the top of the coconut
is creamy white or a whitish yellow. When the
Scientific Name and Introduction area surrounding the cap is green, the coconut
is considered mature and is 10 to 12 mo old. At
Coconut (Cocos nucifera L.) is marketed at two maturity the skin begins to change from green to
stages of development. At an immature stage, the yellow, then brown, and the “rat-tail” is entirely
fruit (water coconut) contains mainly water and a brown.
little jellylike meat instead of the hard, white flesh
(meat/endosperm) found in mature coconuts. In
Thailand, and now marketed in the United States, Grades, Sizes, and Packaging
immature green nuts are trimmed and shaped,
removing most of the husk. The final product has There are no specific grades; informal grades are
a flat bottom, round body with a pyramid top, generally based on size and weight. Mature U.S.
and the eyes showing. To prevent browning of dehusked coconuts are sold in 34- to 36-kg (75- to
the remaining husk, nuts are dipped in 1 to 3% 80-lb) woven plastic or burlap sacks containing 40
sodium meta-bisulfite for 2 to 5 min and then to 50 coconuts, plastic mesh bags of 12 coconuts,
wrapped in plastic film (Tongdee et al. 1991). and cartons with 20 to 25 film-wrapped coconuts
Sometimes fungicide is included in the sulfite weighing 17 to 18 kg (37 to 40 lbs). Immature
solution. Alternatively, the husk is removed before coconuts (water coconuts) are shaped, dipped in
dipping in sulfite solution. Fruit are available year bisulphate, and film-wrapped. They are sold in
round from most tropical countries (Seelig 1970). single-piece cartons containing 10 to 16 nuts. For
young coconuts, the entire husk is removed, and
the nuts are then dipped in sodium bisulfite before
Quality Characteristics and Criteria packing.
294
Optimum Storage Conditions Retail Outlet Display Considerations
Mature coconuts with husk can be kept at Display coconuts at ambient temperatures and do
ambient conditions for 3 to 5 mo before the liquid not mist. Nonwrapped or individually wrapped
endosperm evaporates or before the shell cracks shaped coconuts are displayed at ambient
due to desiccation or sprouting. Storage at 0 to temperatures or at 10 °C (50 °F).
1.5 ºC (32 to 35 ºF) and 75 to 85% RH is possible
for up to 60 days for mature, dehusked coconuts
(Maliyar and Marar 1963), and 13 to 16 ºC (55 to Chilling Sensitivity
60 ºF) and 80 to 85% RH for 2 weeks or less. Low
RH and high temperature should be avoided. When stored at 0 °C (32 °F), immature nuts have
green skins that turn brown after 7 days. Few
Young coconuts are normally held at 3 to 6 ºC (37 other changes occur in other quality characteristics
to 43 ºF) with 90 to 95% RH, while wrapped and at this temperature (Consignado et al. 1976).
shaped fruit can be held for 3 to 4 weeks. Shaped
young coconuts treated with 0.5 to 1.0% sodium
meta-bisulfite can be held at ambient temperature Ethylene Production and Sensitivity
for 2 days before browning occurs, while those
treated with 2% sodium meta-bisulfite can be held Ethylene production is very low to near zero for
at ambient temperature for 2 to 7 days (Tongdee mature husked coconut. There are no reports of
et al. 1992). Young coconuts that have not been sensitivity to ethylene.
dehusked can be stored for a longer period than
dehusked or shaped young coconuts. In dehusked
or shaped coconuts, soluble solids content (SSC) Respiration Rates
declines and total acidity (TA) increases more
rapidly than in nondehusked coconuts. Thus, the Temperature mg CO2 kg-1 h-1
taste of dehusked or shaped coconuts sours more Immature 10 oC 7 to 8
o
rapidly than nondehusked coconuts during storage 20 C 13 to 24
(Somboonsup 1985). The husk acts as an insulator Mature 10 oC 6 to 7
and may increase the storage life of young 20 oC 13 to 26
coconuts.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 1.9 at 10 °C (50 °F), and 1.8 at 20 °C (68 °F).
Controlled Atmosphere (CA) To calculate heat production, multiply mg kg-1 h-1
Considerations by 220 to get BTU per ton per day or by 61 to get
kcal per tonne per day.
No data are available on CA storage. Mature
dehusked coconuts are waxed or film-wrapped to
reduce water loss. Immature husked nuts can also Physiological Disorders
be film-wrapped or waxed; however, the outside
color changes rapidly from white to brown unless Mechanical damage to immature coconuts will
they are dipped in sodium bisulfite (Tongdee et al. cause the white coir (the stiff fiber from the outer
1992). husk) to turn brown and the nut to crack. Younger
nuts have a lower rupture force than mature nuts
(Tongdee 1991). A rapid temperature change of
8 °C (15 °F) or more during storage of mature
husked coconut can lead to cracking (Burton
1982), while freezing occurs at -3 °C (27 °F).
295
Moisture loss causes a loss of water in the nut; this Muliyar, M.K., and M.M.K. Marar. 1963. Studies
loss can be reduced by RH control, film wrapping, on the keeping quality of ripe coconuts in storage.
or waxing of mature nuts. Indian Coconut J. 17:13-18.
References
296
Cranberry Grades, Sizes, and Packaging
297
Controlled Atmosphere (CA) Ethylene Production and Sensitivity
Considerations
Cranberry has a low ethylene production rate of
There is no known commercial use of CA in 0.1 to 1.0 μL kg-1 h-1 at 5 °C (41 °F) (Kader 1992,
cranberry storage. Some research suggests CA Mitcham et al. 1999). Postharvest treatment of
containing various combinations of O2 and CO2 fruit with as little as 10 μL L-1 markedly increases
does not extend cranberry storage life compared anthocyanin content, which is enhanced further if
with ambient air (Anderson et al. 1963, Stark the fruit are treated in the presence of light (Fudge
et al. 1969). Conversely, Kader (1992, 1997) 1930, Craker 1971). Eck (1990) and Reid (1992)
suggested that a CA condition of 1 to 2% O2 and indicate the use of ethephon, a source of ethylene
0 to 5% CO2 is beneficial. The maximum CO2 approved in some areas of the United States,
tolerance may be above 5%, as Stark et al. (1969) which accelerates cranberry maturity and/or red
used 10% CO2 without detrimental effects. In a color development. A 1995 U.S. Environmental
2-mo test at 3 °C (37 °F) with 98% RH using 10 Protection Agency “Reregistration Eligibility
CA combinations of 2%, 21%, and 70% O2 with Decision (RED) on Ethephon” indicates that
0%, 15%, and 30% CO2. Gunes and Watkins cranberries have been deleted from ethephon
(2001) concluded that 21% O2 with 30% CO2 was product labels.
optimal. Cranberries can be held in an anaerobic
condition (100% N2) for up to 14 mo at 3.3 °C (38
°F) in low RH (Stark et al. 1974). Such fruit have Respiration Rates
little decay but a high amount of physiological
breakdown, making them unacceptable for Cranberries have a low respiration rate compared
fresh or juice use but still acceptable for use in to other berry crops, which have a moderate to
cranberry sauce. high rate (Kader 1992).
Cranberry is considered to be a chilling sensitive To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
fruit (Wright et al. 1937, Levine et al. 1941, by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
Kader 1992). Storage at temperatures near 0 °C 1.8 at 20 °C (68 °F). To calculate heat production,
for more than about 4 weeks may result in low- multiply mg kg-1 h-1 by 220 to get BTU ton per
temperature breakdown (Lidster et al. 1988). day or by 61 to get kcal per tonne per day.
Chilling injury symptoms include dull appearance,
rubbery texture, and increased decay (Mitcham
et al. 1999). If fruit are held at 0 °C, intermittent Physiological Disorders
warming to 21 °C (70 °F) for 1 day a month can
reduce chilling injury (Hruschka 1970). Physiological breakdown of cranberry is
manifested by a soft and rubbery condition,
dull external appearance, and diffusion of
red anthocyanin pigment throughout internal
tissues (Ceponis and Stretch 1981). No fungal
organisms are associated with this condition. It
298
is correlated with one or more of the following: Suitability as Fresh-Cut Product
impact bruising; late-harvested, more intensely
colored fruit; and immersion of free berries for 8 No current potential.
h or more in a flooded bog or similar smothering
effects where cranberries are held in poorly
ventilated conditions (Graham et al. 1967, Special Considerations
Patterson et al. 1967, Ceponis and Stretch 1981,
Massey et al. 1981). Chilling injury can have the Cranberries can be stored fresh for 2 to 4 mo,
same symptoms. depending on season, cultivar, maturity, handling,
and storage conditions (Hardenburg et al. 1986).
The storage life of cranberries is limited by the
Postharvest Pathology development of decay, shrinkage resulting from
moisture loss, and physiological breakdown
Postharvest cranberry diseases are almost entirely (Lidster et al. 1988). Early-harvested fruit usually
caused by fungi, with the exception of ringspot, have a longer storage potential than late-harvested
which is thought to be a virus-induced disease fruit (Doughty et al. 1967). Physical damage,
(Caruso and Ramsdell 1995, Prange and DeEll which can occur during mechanical harvesting or
1997). Cranberry is not only attacked by several rough hand-harvesting, transport, or mechanical
common postharvest fungi but also by a large cleaning, sorting, and packing, increases
number of fungi that are unknown on other fruit physiological breakdown, postharvest softening,
crops (Caruso and Ramsdell 1995, Prange and and decay and reduces storage life (Graham et al.
DeEll 1997). The principal storage rots, which 1967, Patterson et al. 1967, Massey et al. 1981).
can be found in all the major cranberry-growing There is more fungal decay and physiological
areas, are end rot, black rot, viscid rot, yellow rot, breakdown in water-harvested than in hand-
and botryosphaeria fruit rot (Eck 1990, Prange harvested cranberries, especially if cranberries are
and DeEll 1997). Since the occurrence of these kept in the water more than 1 h after detachment
fungi can vary with location and season and some from the plant (Mitcham et al. 1999; Blake
are not easily identified visually, confirmation of Johnson, 2000, personal communication).
the causal organism(s) usually requires extensive
culturing and spore examination. Decay may be
reduced if storage O2 is below 1%, since there is Acknowledgments
no decay control at 1% (Anderson et al. 1963), but
100% N2 for 3 weeks at 3 °C (38 °F) reduced the Some of the information in this chapter was
number of pathogenic species and decay compared from the Produce Marketing Association’s
with air-stored fruit (Lockhart et al. 1971). “Fresh Produce Manual” and from B. Johnson
of Bezanson and Chase Cranberry Company,
Aylesford, Nova Scotia, Canada.
Quarantine Issues
299
Anderson, R.E., R.E. Hardenburg, and H.C. Hardenburg, R.E., A.E. Watada, and C.Y.
Vaught. 1963. Controlled atmosphere storage Wang. 1986. The Commercial Storage of Fruits,
studies with cranberries. Proc. Amer. Soc. Hort. Vegetables, and Florist and Nursery Stocks.
Sci. 83:416-422. Agriculture Handbook 66, U.S. Department
of Agriculture, Agricultural Research Service,
Caruso, F.L., and D.C. Ramsdell. 1995. Washington, DC.
Compendium of Blueberry and Cranberry
Diseases. APS Press, St. Paul, MN. Hruschka, H.W. 1970. Physiological breakdown in
cranberries—inhibition by intermittent warming
Ceponis, M.J., and A.W. Stretch. 1981. The during cold storage. Plant Dis. Rptr. 54:219-222.
influence of water immersion time at harvest
on physiological breakdown of ‘Early Black’ Kader, A.A. 1992. Postharvest biology and
cranberries in storage. HortScience 16:60-61. technology: an overview. In A.A. Kader, ed.,
Postharvest Technology of Horticultural Crops,
Craker, L.E. 1971. Postharvest color promotion in pp. 15-20. DANR Pub. no. 3311, University of
cranberry with ethylene. HortScience 6:137-139. California, Davis, CA.
Dana, M.N. 1990. Cranberry management. In Kader, A.A. 1997. A summary of CA requirements
G.J. Galleta and D.G. Himelrick, eds., Small Fruit and recommendations for fruits other than apples
Crop Management, pp. 334-262. Prentice Hall, and pears. In A.A. Kader, 7th International
Upper Saddle River, NJ. Controlled Atmosphere Research Conference,
University of California, Davis, July 13-18, 1997,
Doughty, C.C., M.E. Patterson, and A.Y. Shawa. vol. 3, pp. 1-34. University of California, Davis,
1967. Storage longevity of the ‘McFarlin’ CA.
cranberry as influenced by certain growth
retardants and stage of maturity. Proc. Amer. Soc. Kasmire, R.F., and J.F. Thompson. 1992. Selecting
Hort. Sci. 91:192-204. a cooling method. In A.A. Kader, ed., Postharvest
Technology of Horticultural Crops, pp. 63-68.
Eck, P. 1990. The American Cranberry. Rutgers DANR Pub. no. 3311, University of California,
University Press, New Brunswick, NJ. Davis, CA.
Fudge, B.R. 1930. Increasing the color of Kaufman, J., S.M. Ringel, A.A. Hamer, et al.
cranberries after removal from the vines. Bull. 1958. Effect of Precooling on Market Quality of
504, New Jersey Agricultural Experiment Station, Cranberries Shipped by Rail or Truck. Marketing
New Brunswick, NJ. Research Report 287, U.S. Department of
Agriculture, Agricultural Marketing Service,
Graham, S.O., M.E. Patterson, and B. Allan. 1967. Washington, DC.
Bruising as a predisposing factor in the decay of
stored cranberries. Phytopathology 57:497-501. Levine, A.S., C.R. Fellers, and C.I. Gunness.
1941. Carbon dioxide-oxygen and storage
Gunes, G., and C.B. Watkins. 2001. Postharvest relationships in cranberries. Proc. Amer. Soc.
quality of cranberries as affected by controlled Hort. Sci. 38:239-242.
atmosphere. HortScience 36:571 (Abstract).
Lidster, P.D., P.D. Hildebrand, L.S. Bérard, and
S.W. Porritt. 1988. Commercial Storage of Fruits
and Vegetables. Pub. no. 1532/E, Agriculture and
Agri-Food Canada, Ottawa, Ontario, Canada.
300
Lockhart, CL., F.R. Forsyth, R. Stark, and Stark, R., I.V. Hall, F.R. Forsyth, and P.R. Dean.
I.V. Hall. 1971. Nitrogen gas suppresses 1969. Cranberries evaluated for fresh fruit and
microorganisms on cranberries in short term processing quality after reduced O2 storage.
storage. Phytopathology 61:335-336. Cranberries 34(6):14,16 and 34(7):14,16.
Massey, L.M., B.R. Chase, and M.S. Starr. 1981. Stark, R., F.R. Forsyth, C.L. Lockhart, and I.V.
Impact-induced breakdown in commercially Hall. 1974. Processing quality of cranberries after
screened ‘Howes’ cranberries. J. Amer. Soc. Hort. extended storage in N2 atmosphere with low and
Sci. 106:200-203. high relative humidities. Can. Inst. Food Sci.
Technol. J. 7:9-10.
Mitcham, E.J., C.H. Crisosto, and A.A. Kader.
1999. Bushberries: blackberry, blueberry, Wright, R.C., J.B. Demaree, and M.S. Wilcox.
cranberry, raspberry. In Fresh Produce Facts. 1937. Some effects of different storage
At http://postharvest.ucdavis.edu/produce_ temperatures on the keeping of cranberries. Proc.
information. Amer. Soc. Hort. Sci. 34:397-401.
301
Cucumber (2 in) for picklers and <6 cm (2 3/8 in) for slicers.
The minimum length is 14 cm (5.5 in) for slicers.
Mikal E. Saltveit Fruit firmness, external glossiness, and formation
of jellylike material around the seeds are
Saltveit is with the Department of Plant Sciences, indicators of proper harvest maturity. Greenhouse-
Mann Laboratory, University of California, Davis, grown parthenocarpic (produced without
CA. pollination) fruit are harvested 10 to 14 days after
anthesis after they turn bright green (Kanellis et
al. 1988). Straight, uniformly cylindrical fruit
Scientific Name and Introduction slightly tapered at both ends are of highest quality.
Cucumbers are listed as nonclimacteric, yet there
Cucumber (Cucumis sativus L) is a member of is a burst of ethylene production that precedes
the Cucurbitaceae family, along with melons, a rapid loss of chlorophyll in harvested fruit
squashes, and many other horticulturally important (Saltveit and McFeeters 1980).
species. The fruit are borne on indeterminate,
tendril-bearing vines of subtropical and tropical
origin (Robinson and Decker-Walters 1997). The Grades, Sizes, and Packaging
inferior ovary has three united carpels in most
cultivars. Compact, determinate cultivars have Grades for table or slicing cucumbers are based on
been developed for home gardeners and for once- length, shape, fruit color, and diameter and include
over mechanical harvesting (Miller and Wehner U.S. Fancy, Extra 1, No. 1, No. 1 Small, No. 1
1989). Large, and No. 2. Pickling cucumbers are graded
by fruit diameter: No. 1 (up to 2.5 cm [1 in]), No.
2 (2.5 to 3.8 cm [1.0 to 1.5 in]), and No. 3 (3.8
Quality Characteristics and Criteria to 5.0 cm [1.5 to 2.0 in]). Fruit over 5 cm (2 in)
are too large. Additional quality indices include
Fruit are round to oblong or narrowly cylindrical, size, freedom from growth or handling defects,
with small tubercles (warts) and spines of freedom from decay, and lack of yellowing. Most
trichome origin on the rind of some cultivars fresh-market cucumbers are packed in fiberboard
(Miller and Wehner 1989). The rind of slicing boxes. Fresh market slicers are often waxed to
cucumbers should be dark green and firm with reduce moisture loss, while greenhouse slicers are
no pits or wrinkled (pinched) ends. Spine color is wrapped in shrink-plastic to reduce water loss.
associated with mature fruit color and fruit netting. Pickling cucumbers are usually transported from
Fruit of white-spined cultivars are light green to the field to the brining facility in large field bins.
yellow when mature and not netted. Black-spined
fruit are orange or brown when mature and may
be netted. The flesh is crisp and white, except in a Precooling Conditions
few cultivars where it is pale orange.
The chilling sensitivity of cucumbers does
not preclude precooling with cold water
Horticultural Maturity Indices (hydrocooling) or air (forced-air) (Ryall and
Lipton 1979). However, even though fruit can
Fruit are harvested at various stages of tolerate brief exposure to chilling temperatures,
development. Immature fruit are green at the they should not be maintained at chilling
edible stage, except for a few cultivars that are temperatures for more than 6 h.
white or yellow. Fruit are generally harvested
immature (that is, before the seeds are fully
enlarged and hardened) with a diameter of <5 cm
302
Optimum Storage Conditions temperatures for 12 h every 2 or 3 days can reduce
chilling injury of fruit held at 0 to 2 °C (32 to 36
Recommended conditions for commercial storage °F) (Cabrera and Saltveit 1990). Development of
of cucumbers are 10 to 12.5 ºC (50 to 54 °F) chilling injury symptoms can be avoided if chilled
at 95% RH (Hardenburg et al. 1986). Storage fruit are used immediately after removal from
life is generally less than 14 days, with visual storage.
and sensory quality rapidly declining thereafter.
Chilling sensitivity limits storage temperatures
to a narrow range. Storage below 10 °C (50 °F) Ethylene Production and Sensitivity
results in chilling injury in as little as 2 or 3 days,
while storage at 15 °C (59 °F) results in rapid Cucumber fruit produce little ethylene, about
yellowing and loss of quality. 0.1 to 1.0 µL kg-1 h-1 at 20 °C (68 °F), but they
are very sensitive to it. Exposure to 1 to 5 µL
L-1 ethylene accelerates yellowing and decay.
Controlled Atmosphere (CA) Reduced O2 and elevated CO2 minimize the
Considerations response to ethylene exposure.
None. References
Cucumber fruit are usually treated with approved Hardenburg, R.E., A.E. Watada, and C.Y.
waxes or oils to reduce water loss, reduce abrasion Wang. 1986. The Commercial Storage of Fruits,
injury, and improve appearance. Surface pitting Vegetables, and Florist and Nursery Stocks.
and yellowing are common defects that follow Agriculture Handbook 66, U.S. Department
exposure to chilling temperatures and ethylene. of Agriculture, Agricultural Research Service,
Harvesting at the proper maturity and storing at Washington, DC.
temperatures above 10 °C (50 °F) in an ethylene-
free atmosphere are necessary for optimal quality Kanellis, A.K., L.L. Morris, and M.E. Saltveit.
retention and maximum market life. 1988. Responses of parthenocarpic cucumbers
to low-oxygen storage. J. Amer. Soc. Horti. Sci.
The postharvest conditions for slicing cucumbers 113:734-737.
generally apply to fruit intended for pickling.
However, damage incurred during the mechanical Leshuk, J.A., and M.E. Saltveit. 1990. Controlled
harvesting of pickling cultivars increases atmospheres and modified atmospheres for
the rate of respiration (from 6 to 20%), the preservation of vegetables. In M. Calderon, ed.,
rate of water loss, and disease susceptibility. Food Preservation by Modified Atmospheres, pp.
Also, since pickling cucumbers are commonly 315-352. CRC Press, Boca Raton, FL.
shipped in large field bins, prompt precooling
is very important to remove field heat and Miller, C.H., and T.C. Wehner. 1989. Cucumbers.
prevent respiratory activity from raising fruit In N.A.M. Eskin, ed., Quality and Preservation of
temperature in transit. Hydrocooling is effective Vegetables, pp. 245-264. CRC Press, Boca Raton,
for precooling, but storage life is halved because FL.
of the spread of inoculum. Sanitization of water
is therefore very important. Fruit can be held Ryall, A.L., and W.J. Lipton. 1979. Handling,
at chilling temperatures for a few days if used Transportation and Storage of Fruits and
immediately after removal from storage. Vegetables, 2nd ed., vol. 1, Vegetables and
Melons. AVI Publishing Co., Westport, CT.
304
Robinson, R.W., and D.S. Decker-Walters. 1997.
Cucurbits. CAB Intl., Wallingford, U.K.
305
Currant, Gooseberry, and Elderberry Also, brownish‑purple fruit from a native western
U.S. species, R. aureum Pursh (buffalo currant,
Robert K. Prange albol currant, golden currant) or from a hybrid of
R. nigrum and R. hirtellum (jostaberry) may be
Prange is with Atlantic Food and Horticulture available in some local markets.
Research Centre, Agriculture and Agri-Food
Canada, Kentville, Nova Scotia, Canada. Sambucus canadensis L., the elderberry,
is a moderately tall deciduous shrub of the
Caprifoliaceae family, native to North America.
Scientific Name and Introduction The ease in harvesting the purplish-black fruit
from wild plants may in part account for the
Currants and gooseberries are closely related, lack of commercial plantings. Most of the fruit
berry‑bearing deciduous shrubs in the Ribes L. is processed, since the uncooked berries are
genus of the Saxifragaceae family. Gooseberries astringent and not very edible (Way 1981).
are sometimes placed in a separate genus, Selection and breeding, primarily in New York
Grossularia Mill. and Nova Scotia, have resulted in a number of
cultivars (Craig 1978, Way 1981, Stang 1990), and
The most common species are R. sativum Syme small-scale production has been reported in New
(red, white, and pink currants) and R. nigrum York, Ohio, and Oregon (Way 1981).
L. (black currant) (Harmat et al. 1990). White
currant, an albino form of red currant, is of lower
acidity and thus is suitable for eating fresh. Quality Characteristics and Criteria
Pink currants have a colorless skin and a pink
flesh. Black currant fruit differ from red currants Currant and elderberry fruit are produced on the
in being more astringent but having a distinct plant in clusters, and gooseberry fruit are borne
aroma, making it very desirable in processed singly or in pairs. Ideally, fruit throughout each
products. Gooseberry cultivars are derived from cluster should be firm and bright, with the proper
R. uva‑crispa L. (European gooseberry) and R. cultivar‑specific color, and free of decay or
hirtellum Michx. (American gooseberry). The mechanical or insect injury. For the fresh market,
European gooseberry is much larger than the it is also important to have large and uniform
American gooseberry. European cultivars are from fruit throughout the cluster. A long shelf‑life
R. uva‑crispa, but American cultivars are virtually with retention of both firmness and flavor is also
all from crosses of R. uva‑crispa and R. hirtellum. desirable for the fresh-fruit market.
306
mature first (Audette and Lareau 1996, http:// Optimum Storage Conditions
www.crfg.org/pubs/ff). The entire cluster can be
harvested, or only mature berries can be picked Since currant, gooseberry, and elderberry are
over several harvests. At maturity, gooseberry not chilling sensitive, the recommended storage
cultivars may be green, white, yellow, or various temperature and RH for all three are ‑0.5 to 0
shades of red (pink to purple to almost black). °C (31 to 32 °F) with RH of 95% (Hardenburg
Since both immature (green) and ripe gooseberries et al. 1986, Story and Simons 1989). Batzer and
are used, harvest maturity depends entirely on end Helm (1999) recommended slightly warmer
use (Ryall and Pentzer 1982). Green gooseberries temperatures of 0 to 1 °C (32 to 34 °C) for red
are very firm and tart, whereas some cultivars, currant and gooseberry and 0 to 2 °C (32 to 36
when fully mature and soft, are quite sweet. °F) for black currant, perhaps to avoid accidental
Elderberries are harvested in late August and freezing. With proper cooling, the storage duration
September, when the fruit is sufficiently large can be 1.5 , 2.5, and 3 weeks for black currant, red
and has changed to an acceptable purplish‑black currant, and gooseberry, respectively (Batzer and
color. The fruit do not mature at the same time, so Helm 1999).
several pickings are necessary over a 1- to 2-week
period (Craig 1978, Way 1981). Harvesting occurs
in late August and September, depending on Controlled Atmosphere (CA)
climate and cultivar. Postharvest decay of currant, Considerations
gooseberry, and elderberry can be minimized by
avoiding picking wet or overripe fruit. As summarized by Batzer and Helm (1999) and
Thompson (1998), research indicates that red
currant and gooseberry respond very well to CA,
Grades, Sizes, and Packaging while black currant benefits only slightly. Storage
duration of red currant can be extended to 8 to 14
There are no U.S. fresh fruit standards for weeks, depending on cultivar, using 18 to 20%
these fruit. There is a U.S. grade standard for CO2 with 2% O2 at 1 °C (34 °F). For gooseberry,
processing currants, based on color, attachment storage duration is extended to 6 to 8 weeks using
of stem, and freedom from decay and insect or 10 to 15% CO2 with 1.5% O2 at 1 °C. Increasing
mechanical damage. Processors, who use most of the CO2 up to 20% reduces incidence of storage
the commercial production, may have their own rots (Thompson 1998, Batzer and Helm 1999),
standards. Since fresh market volumes are not and lowering the O2 reduces respiration rate
large, container sizes and packaging for the fresh (Robinson et al. 1975). Compared with red currant
market tend to be those used for similar but more and gooseberry, black currant does not respond
common berries (for example, raspberries). as well to low O2 and its storage can only be
extended to 3 weeks at 0 to 2 °C (32 to 36 °F) and
15 to 20% CO2.
Precooling Conditions
There is no known information on the effect of CA
Currant, gooseberry, and elderberry fruit are on elderberry.
relatively perishable. Quick cooling after harvest
to recommended storage temperatures is desirable,
using forced‑air cooling with 95% RH (Kasmire Retail Outlet Display Considerations
and Thompson 1992, Batzer and Helm 1999).
Currant, gooseberry, and elderberry should be kept
in a refrigerated display but not sprinkled with
water or top-iced.
307
Chilling Sensitivity Postharvest Pathology
Currant, gooseberry, and elderberry are not The main postharvest disease is gray mold rot
chilling sensitive (Kader 1992). (Botrytis cinerea), which can appear as small
brown spots on currant and gooseberry fruit
(Ryall and Pentzer 1982, Dennis 1983, Harmat et
Ethylene Production and Sensitivity al. 1990). These enlarge rapidly at temperatures
above 10 °C (50 °F) and gradually affect the entire
No data available. berry with a soft rot. Currant and gooseberry
fruit can be susceptible to American powdery
mildew (Sphaerotheca mors-uva) (Harmat et
Respiration Rates al. 1990, Audette and Lareau 1996). Fruit that
become contaminated by soil splash after heavy
Temperature Gooseberry Black currant rain are frequently infected by Mucor piriformis
---------mg CO2 kg-1 h-1--------- (Dennis 1983). Dennis (1983) also reports a fruit
0 °C 5 to 7 16 disease in gooseberry caused by Alternaria and
4 to 5 °C 8 to 16 27 Stemphyllum. The infection is usually confined
10°C 12 to 32 40 to the seeds enclosed in the pericarp. If, however,
15 to 16°C 28 to 69 89 the fruit is stored for a few days at ambient
20 to 21°C 41 to 105 128 temperatures prior to consumption or processing,
Gooseberry data from Smith (1967), Robinson the fungus invades the pericarp tissue.
et al. (1975), and Hardenburg et al. (1986); black
currant data from Robinson et al. (1975). Several insects can attack the fruit: currant maggot
or fruit fly (Epochra canadensis) (North America),
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate gooseberry fruitworm (Zophodia convolutella)
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and (North America), and currant moth (Incurvaria
1.8 at 20 °C (68 °F). To calculate heat production, capitella) (Europe) (Harmat et al. 1990). In
multiply mg kg-1 h-1 by 220 to get BTU per ton per addition, slugs and snails (Helix aspersa and
day or by 61 to get kcal per tonne per day. Cepaea spp.) will attack fruit (North America
and Europe) (Harmat et al. 1990). Diseases and
insects are not generally serious on elderberries
Physiological Disorders (Way 1981), perhaps because of the absence
of extensive plantings. An unidentified mildew
CO2 above 20% results in internal breakdown and can be a problem on ripe fruit, especially if the
fruit discoloration in some red currant cultivars weather is cool during ripening and there is poor
after 13 weeks of storage (Thompson 1998). air circulation around the plants (Way 1981).
Low O2 further increases these symptoms. Smith
(1967) showed that green gooseberry fruit held at Birds feeding on ripe currant, gooseberry, and
0 °C (32 °F) in air are damaged by CO2 that was elderberry fruit can be a serious pest problem
increased from 8 to 12%. Fruit turned yellow and (Way 1981, Harmat et al. 1990, Stang 1990).
had an abnormal flavor. Increasing the temperature In addition to prompt harvesting of ripe fruit,
from 0 to 5 °C (32 to 41 °F) eliminates the various bird repellent measures may have to be
disorder. considered.
Quarantine Issues
None
308
Suitability as Fresh‑Cut Product Harmat, L., A Porpaczy, D.G. Himelrick, and
G.J. Galleta. 1990. Currant and gooseberry
No current potential management. In G.J. Galleta and D.G. Himelrick,
eds., Small Fruit Crop Management, pp. 245‑272.
Prentice Hall, Upper Saddle River, NJ.
Special Considerations
Kader, A.A. 1992. Postharvest biology and
Ribes species are hosts for the white pine blister technology: an overview. In A.A. Kader, ed.,
rust, which causes few problems for currants or Postharvest Technology of Horticultural Crops,
gooseberries but is dangerous to 5‑needle pine pp. 15-20. DANR Pub. no. 3311, University of
species. Thus, commercial production of Ribes California, Davis, CA.
species, especially black currant, may be banned
in some U.S. municipalities (California Rare Fruit Kasmire, R.F., and J.F. Thompson. 1992. Selecting
Growers 1996). a cooling method. In A.A. Kader, ed., Postharvest
Technology of Horticultural Crops, pp. 63-68.
DANR Pub. no. 3311, University of California,
Davis, CA.
References
Robinson, J.E., K.M. Browne, and W.G. Burton.
Audette, M., and M.J. Lareau. 1996. Currants
1975. Storage characteristics of some vegetables
and Gooseberries Culture Guide. Conseil des
and soft fruits. Ann. Appl. Biol. 81:339-408.
Productions Végétales du Québec, Inc., Québec
City, Québec, Canada.
Ryall, A.L., and W.T. Pentzer. 1982. Handling,
Transportation and Storage of Fruits and
Batzer, U., and H.‑U. Helm. 1999. Lagerung
Vegetables, 2nd ed., vol. 2, Fruits and Tree Nuts.
von Beerenobst (Storage of small fruits).
AVI Publishing Co., Westport, CT.
Erwerbsobstbau 41:51‑55.
Spayd, S.E., J.R. Morris, W.E. Ballinger, and D.G.
California Rare Fruit Growers. 1996. CRFG fruit
Himelrick. 1990. Maturity standards, harvesting,
facts: currants. At http://www.crfg.org/pubs/ff/
postharvest handling, and storage. In G.J. Galleta
currants.
and D.G. Himelrick, eds., Small Fruit Crop
Management, pp. 504‑531. Prentice Hall, Upper
Craig, D.L. 1978. Elderberry culture in eastern
Saddle River, NJ.
Canada. Pub. no. 1280, Agriculture Canada,
Ottawa, Ontario, Canada.
Smith, W.H. 1967. The storage of gooseberries.
Agricultural Research Council (U.K.) Ditton and
Dennis, C. 1983. Soft fruits. In C. Dennis, ed.,
Covent Garden Laboratories Annual Report 1965-
Postharvest Pathology of Fruits and Vegetables,
66, pp. 13-14.
pp. 23-42. Academic Press, New York, NY.
Stang, E.J. 1990. Elderberry, highbush cranberry,
Hardenburg, R.E., A.E. Watada, and C.Y.
and juneberry management. In G.J. Galleta
Wang. 1986. The Commercial Storage of Fruits,
and D.G. Himelrick, eds., Small Fruit Crop
Vegetables, and Florist and Nursery Stocks.
Management, pp. 363‑382. Prentice Hall, Upper
Agriculture Handbook 66, U.S. Department
Saddle River, NJ.
of Agriculture, Agricultural Research Service,
Washington, DC.
Story, A., and D.H. Simons. 1989. Handling and
Storage Practices for Fresh Fruit and Vegetables.
Australian United Fresh Fruit and Vegetable
Association Ltd., Victoria, Australia.
309
Thompson, A.K. 1998. Controlled Atmosphere
Storage of Fruits and Vegetables. CAB Intl.,
Wallingford, U.K.
310
Date high sugar content can be harvested at the khalal
stage (“balah” in North African countries or
Elhadi M. Yahia “bisr” in Oman). For other varieties, dates that are
harvested before reaching full maturity must be
Yahia is with the Faculty of Natural Sciences, ripened artificially. Very immature dates cannot
Universidad Autónoma de Querétaro, Querétaro, be properly ripened artificially and will thus be of
Mexico. poor quality.
Phoenix dactylifera L., the date palm, has been Dates are graded based on uniformity of color
a staple in the Middle East and North Africa for and size and extent of defects or damage, which
thousands of years. Date fruit is a drupe with a include discoloration of the flesh, rupture of
single seed or pit. The fruit has an oblong shape, the skin, deformity of the fruit, puffiness of
measures about 2.5 to 7.5 cm (1 to 3 in) long, and the skin, scars, sunburn, insect damage, decay,
has thick or thin flesh. It tastes astringent when black scald, fermentation, improper ripening,
premature and becomes sweet when ripe. Dates mechanical damage, and presence of dirt or other
are rich in carbohydrate and other nutrients and foreign material. These criteria are the bases for
are a high-energy food. Codex and U.S. grades A, B, C, standard, and
substandard applied to whole, pitted, or dry dates.
In general, the total amounts of sugar for different
Quality Characteristics and Criteria grades are usually the same when expressed as a
percentage of dry weight, but the higher grades
High-quality fresh dates should be of an adequate usually contain higher amounts of sugar per date.
size and proper color and have a small pit and ‘Medjool’ dates in the United States are classified
thick flesh. They should not be contaminated by into three size categories: jumbo (fewer than 10
dirt, sand, or leaf particles or exhibit damage by dates per lb or per 0.45 kg), mixed (10 to 15 dates
birds, insects, or rodents. There should be no fungi per lb), and conventional (more than 15 dates per
or mold infestation nor sugar crystal formation lb).
or other apparent alterations (Dowson 1982). The
skin should be smooth with little or no shriveling. Some dates are marketed in 15-lb (6.8-kg) flats
The color should be golden brown, amber, green, of fiberboard or wood, others in 5- or 10-lb (2.3-
or black, depending on the variety. The texture or 4.5-kg) cartons. Large, reinforced cartons are
may be soft and syrupy or firm and dry, depending used for packing dry dates, especially for export.
on the cultivar. Consumer packages are widely used and consist
of various sizes and shapes, including bags of
transparent film and trays overwrapped with
Horticultural Maturity Indices film. Round fiberboard cans with metal tops and
bottoms containing 500 to 1,000 g (1.1 to 2.2 lb)
Fruit growth follows a sigmoidal curve and is are also used. Rigid, transparent plastic containers
usually divided into five stages of development with a capacity of 200 to 300 g (0.44 to 0.66 lb)
known by their Arabic terms: hababouk, kimri, are commonly used. Small consumer packages
khalal, rutab, and tamr. Most dates are harvested are also used, such as bags containing 50 to 60 g
in the tamr stage when the fruit has about 60 to (about 2 oz).
80% sugar content, depending on location and
cultivars. At this stage, fruit can be harvested
soft, semidry, or dry depending on destination
and use. Some varieties with low tannins but
311
Optimum Storage Conditions the fruit increases. Cured ‘Deglet Noor’ dates,
with 20 to 22% moisture, produced 0.4 mg CO2
Pathological and physiological deterioration kg-1 h-1 at 24 °C and 2 mg CO2 kg-1 h-1 when
increases with increasing moisture content and moisture content increased to 27%. (Rygg 1975).
storage temperature. Relatively small differences
in moisture content may have an important effect
on keeping the quality of ‘Deglet Noor’ fruit. Physiological Disorders
At 24 °C (75 °F), ‘Deglet Noor’ dates show
darkening of the skin 4 times faster when they Darkening is a major problem in dates. Its
have 24% versus 20% moisture content (Rygg rate varies with cultivar, temperature, and
1975). At 0 °C (32 °F), dates can be stored in good moisture content; and it is affected by several
conditions up to 1 year, but some varieties may treatments. It can be reduced by storage in low
develop sugar spots or crystals. Fully mature soft temperatures, low moisture content, or in an inert
and firm ‘Deglet Noor’ dates can be kept for more gas. Temperatures above 60 °C (140 °F) cause a
than 1 year when stored at -17.5 °C (0 °F) but will reddish color and increase the astringency and off
not stand more than 1 mo at 27 °C (80 °F), 3 mo flavors in ‘Deglet Noor.’
at 15 °C (59 °F), or 8 mo at 5 °C (41 °F) (Rygg
1956). Partially dried dates can be kept for 1 year Blacknose, or sugartip, is a severe checking of the
at 0 °C (32 °F) or lower, or for a few weeks at skin in some cultivars, especially in ‘Deglet Noor.’
ambient temperature. Dry dates can be kept at 20 It is induced by high RH just before the beginning
°C (68 °F) for years without significant quality of the khalal stage and is characterized by
losses. Optimum RH is 70 to 75%. darkening, shriveling, and hardening of the flesh
at the tip of the fruit (Rygg 1975). Black scald
is characterized by blackening of the flesh and a
Chilling Sensitivity sunken area with a definite line of demarcation at
the tip or on the sides of the fruit.
Ripe dates at the rutab or tamr stages (see
Horticultural Maturity Indices), commonly Puffiness, or sunken separation, is caused by high
handled in the world market, are not sensitive temperatures and/or high RH before the beginning
to chilling and freezing temperatures. However, of ripening and may increase during curing. This
freezing temperatures can injure dates at early disorder affects only soft cultivars.
stages of kimri and khalal.
Sugar spotting is characterized by light-colored
spots under the skin and is restricted to the invert
Ethylene Production and Sensitivity sugar dates. Almost all dry cultivars, and several
of the semidry cultivars, contain large amounts of
At 20 °C (68 °F), dates produce less than 0.1 µL sucrose and are less sensitive to sugar spotting.
kg-1 h-1 ethylene at the khalal stage and none at the Sugar spotting decreases as the temperature
rutab and tamr stages. Ripe dates are not sensitive decreases and when moisture falls below 22%.
to ethylene exposure. Sugar spots affect appearance and texture. They
can be removed by warming but can reappear if
unfavorable conditions persist (Rygg 1975).
Respiration Rates
Freezing of dates at a higher temperature
Respiration rates of dates are very low: <5 mg range results in the rupture of various cellular
CO2 kg-1 h-1 at 20 °C (68 °F) at the khalal stage compartments and the appearance of bright,
and <2 mg kg-1 h-1 at the rutab and tamr stages. yellow-brown spots of crystallized solutes
Respiration rates increase as moisture content of (Shomer et al. 1998). Intercellular membranes
312
and cell walls can be kept intact even after 10 Quarantine Issues
mo when fruits are frozen at a lower temperature
range. Fumigation by phosphine, ionizing radiation,
the use of low or high temperatures, and MA
treatments are registered to be used for insect
Postharvest Pathology control in dates.
References
Insect Pests
Dowson, V.H.W. 1982. Date production and
Oligonychus afrasiaticus McGregor and O. protection with special reference to North Africa
pratensis Banks are mites referred to as “Bou and the Near East. Plant Production and Protection
Faroua” or “Old World date mite.” They affect Paper 35, United Nations, Food and Agriculture
dates at the hababouk stage. The larva develops Organization, Rome, Italy.
around the fruit with a white filament netting,
which in turn causes the fruit to drop prematurely. Rygg, G.L. 1975. Date Development, Handling
The same is caused by Coccotrypes daclyliperda, and Packing in the United States. Agriculture
which leads to fruit drop-off at the immature Handbook 482, U.S. Department of Agriculture,
green stage. Parlatoria blanchardi scale also Agricultural Research Service, Washington, DC.
attacks the fruit while it is still green. Date or
carob moth Ectomyelois ceratoniae Zeller, another Rygg, G.L. 1956. Effect of temperature and
Lepidoptera widely present in different date- moisture content on the rate of deterioration in
producing areas, causes important postharvest Deglet Noor dates. Date Growers’ Institute Report
losses on stored dates. Several other insects, 33.
such as Batrachedra amydraula Meyr, date stone
beetle (Coccotrypes dactyliperda F.), Carpophilus
hemipterius, Carpophilus multilatus, Urophorus Further Reading
humeralis, and Heptoncus luteolus, can cause
serious damage to dates on the bunch or after Abbas, M.F., and M.A. Ibrahim. 1996. The role of
harvest. Other pests include Vespa orientalis, ethylene in the regulation of fruit ripening in the
Cadra figulilella, Arenipes sabella, and mushroom Hillawi date palm (Phoenix dactylifera). J. Sci.
mites (Tyrophagus lintaeri Osborn), which can Food Agric. 72:306-308.
infest stored dates.
313
Ahmed, M.S.H., Z.S. Al-Hakkak, S.R. Ali, et al. Rygg, G.L., J.R. Furr, R.W. Nixon, and W.W.
1982. Disinfection of commercially packed dates, Armstrong. 1953. Factors affecting the spoilage of
Zahdi, by ionizing radiation. Date Palm J. 1:249. dates at room temperature. Date Growers’ Institute
Report 30:10-14.
Al-Taweel, A.A., M.S.H. Ahmed, M.A. Shawki,
and M.J. Nasser. 1993. Effects of sublethal Shomer, I., H. Borchov-Neori, B. Luzki, and
doses of gamma radiation on the mating ability U. Merin. 1998. Morphological, structural and
and spermatophore transfer of Ephesia cautella membrane changes in frozen tissues of Madjhoul
(Lepidoptera: Pyralidae). Insect Sci. Appl. 14:7- date (Phoenix dactylifera L.) fruits. Postharv. Biol.
10. Technol. 14:207-215.
Barreveld, W.H. 1993. Date palm product. Vandercook, C.E., S. Hasegawa, and V.P. Maier.
Agric. Serv. Bull.101, United Nations, Food and 1980. Dates. In S. Nagy and P.E. Shaw, eds.,
Agriculture Organization, Rome, Italy. Tropical and Subtropical Fruits. AVI Publishing
Co., Westport, CT.
Benjamin, N.D., H.R. Shabana, A.A. Mahdi
Hassan, et al. 1976. Effect of different
temperatures and types of containers on the
storage of commercial varieties of dates in Iraq.
Technical Bull. 13, Palm and Dates Research
Center, Baghdad, Iraq.
314
Dragon Fruit skin color change occurs, then declines 25 to 30
days after flowering (Nerd et al. 1999, Le et al.
Robert E. Paull 2000). At this stage, soluble solids content (SSC)
increases to about 14% (Nerd et al. 1999, Le et al.
Paull is with the Department of Tropical Plant and 2000).
Soil Sciences, University of Hawaii at Manoa,
Honolulu, HI.
Horticultural Maturity Indices
Scientific Name and Introduction A common index of maturity is skin color change
to almost full red (Nerd et al. 1999). Harvesting
Dragon fruit (Hylocereus spp.), also known as indices include color, SSC, total acidity (TA),
strawberry pear or thang loy (Vietnamese), pitaya and days from flowering (minimum 32 days). An
roja (Spanish), and la pitahaya rouge (French), SSC:TA of 40 has been suggested as a harvest
grows on a tropical climbing cactus. There is some index.
confusion as to what species is being grown as
they are all referred to as “pitahaya” in Spanish.
The normally white-fleshed Hylocereus undatus Grades, Sizes, and Packaging
is grown commercially, as are the red- or purple-
fleshed H. costaricensis (grown in Nicaragua There are no U.S. or international standards.
and possibly Guatemala) and H. polyhizus Fruit are generally graded by size and color. Size
(grown in Israel). There are yellow clones of H. grades suggested for Vietnam are extra large (over
undatus named “pitaya amarilla” (yellow pitaya) 1.1 lb or 500 g), large (0.84 lb-1.1 lb or 380-500
in Mexico and other Latin-American countries. g), regular (0.66-0.84 lb or 300-380 g), medium
Pitaya amarilla is a different species from the (0.57-0.66 lb or 260-300 g), and small (under 0.57
other yellow pitaya, Selenicereus megalanthus lb or 260 g) (Le et al. 2000). Fruit exported from
(Mizrahi et al. 1997). Dragon fruit is a self- Israel to Europe are graded by number of fruit per
compatible cultivar in Vietnam (Mizrahi et al. 8.8-lb (4-kg) cardboard box: 6, 8, 10, 12, 14, and
1997, Nerd and Mizrahi 1997). 16.
Dragon fruit is a large, oblong fruit with a red There are no reported data. Room cooling and
peel and large, green scales. The scales turn hydrocooling are possible.
yellow on ripening. The skin begins to change
color 25 to 30 days after flowering in both H.
undatus and H. polyhizus. At about the same Optimum Storage Conditions
time flesh firmness approaches a minimum, and
eating quality approaches a maximum 33 to 37 The recommended storage temperature for dragon
days after flowering (Nerd et al. 1999). Fruit can fruit is 10 °C (50 °F), since 6 °C (43 °F) can
be harvested 25 to 45 days after flowering; 32 to induce chilling injury (Nerd et al. 1999). The
35 days was recommended by Nerd et al. (1999). lower temperature (6 °C) has been recommended
Fruit size depends on seed number (Weiss et al. for the yellow pitaya Selenicereus megalanthus
1994). (Nerd and Mizrahi 1999), and this agrees with
minimum growth temperature of 7 °C ( 45 °F) for
The flesh of different species can vary from white this species (Nerd and Mizrahi 1998). Dragon fruit
to various hues of red to very dark red. As the has a storage life of about 14 days at 10 °C (50
fruit matures, acidity reaches a peak just as the °F); while at 5 °C (41 °F) and 90% RH, a storage
315
life of 17 days can be achieved (Le et al. 2000) if Respiration Rates
fruit are harvested 30 to 35 days after flowering.
However, 5 °C (41 °F) may lead to chilling injury The maximum respiration rate of these
on return to 20 °C (68 °F), as indicated by peel nonclimacteric fruit (H. undatus and H. polyhizus)
and flesh deterioration and inferior taste (Nerd occurs during early fruit growth (Nerd et al. 1999,
et al. 1999). Thus, 10 °C (50 °F) for a maximum Le et al. 2000). The following respiration rates are
of 14 days may be a better recommended storage for mature fruit:
temperature.
Temperature mg CO2 kg-1 h-1
20 °C 95 to 144
Controlled Atmosphere (CA) 23 °C 75 to 100
Considerations Data from Nerd et al. (1999) and Le et al. (2000),
respectively.
No reported CA data are available. Fruit harvested
28 to 30 days after flowering and stored in a To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
modified atmosphere (MA) bag (O2 transmission by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
rate 4,000 mL m-2 day-1) can be held for 35 days 1.8 at 20 °C (68 °F). To calculate heat production,
at 10 °C (50 °F), versus 14 days for air controls multiply mg kg-1 h-1 by 220 to get BTU per ton per
(Le et al. 2000b). More mature fruit (40 days after day or by 61 to get kcal per tonne per day.
flowering) in the same MA bag had 50% of the
shelf-life.
Physiological Disorders
Retail Outlet Display Considerations Chilling injury, mechanical injury, and water loss
are the three major disorders. Mechanical injury
Display dragon fruit at 10 ºC (50 °F). Do not mist. leads to development of sunken areas. More
mature fruit are more susceptible to mechanical
injury (Le et al. 2000). Splitting is a problem
Chilling Sensitivity in fruit more than 35 days after flowering that
received rainfall or excessive irrigation during
Flesh translucency is a symptom of chilling ripening (Le et al. 2000).
injury. Other symptoms include softening, wilting,
darkening of scales, browning of the outer flesh,
and poor flavor. These symptoms rapidly develop Postharvest Pathology
on H. undatus and H. polyhizus fruit held at 6 °C
(42.8 °F) for 2 weeks then transferred to 20 °C Bacterial (Xanthomonas campestris) and
(68 °F) (Nerd et al. 1999). Fruit harvested 25 days Dothiorella spp. diseases have been reported
after flowering are more sensitive to chilling (6 (Barbeau 1990). Postharvest disease has been
°C, 7 days). Sensitivity is significantly reduced associated with Fusarium lateritium, Aspergillus
when fruit are harvested 30 to 35 days after riger, and Aspergillus flavus (Le et al. 2000).
flowering (6 °C, 17 days). No commercially significant bacterial or fungal
diseases have been experienced in Israel.
References
317
Durian sweet pulp (aril). The pulp can be odorless or have
a strong odor suggesting garlic, onion, or pungent
Robert E. Paull and Saichol Ketsa cheese with a hint of fruitiness. The edible pulp
(20 to 35% total mass) has a smooth, custardlike
Paull is with the Department of Tropical Plant and texture. In some fruit, seeds are rudimentary or
Soil Sciences, University of Hawaii at Manoa, small compared with wild types.
Honolulu, HI; Ketsa is with the Department of
Horticulture, Kasetsart University, Bangkok, Quality criteria include a pulp with sweet flavor
Thailand. and good texture, few or small seeds, large aril
percentage, and marketable weight of 1.5 to
3.5 kg (3.3 to 7.7 lb), elongated to round shape,
Scientific Name and Introduction good shelf-life, good rind color and thickness,
reduced rind splitting, and freedom from disease
The durian (Durio zibethinus Murray [syn. D. and insects. Superior varieties have thick, yellow,
acuminatissina Merr]) is often referred to as the fiberless, and custardlike pulp.
“King of Fruits” in Southeast Asia. Its qualities
promote considerable discussion because of its The Thai cultivar ‘Chanee’ has a stronger aroma
unique odor, which can be offensive to some and is smaller than the preferred ‘Monthong’
people. (The fruit has a strong onion-garlic odor.) (Golden Pillow). Malaysian cultivars include
“Zibethinus” is derived from the word “zibetto,” D-2 ‘Dato Nina,’ D-7 ‘Repok B-2,’ D-10 ‘Durian
Italian for the civet, a catlike animal with a musky Hyan,’ and D-24 and D-98 ‘Katoi.’
odor. This tropical tree is mainly cultivated in Sri
Lanka, southern India, southern Burma, Thailand,
Cambodia, Vietnam, Malaysia, Indonesia, Borneo, Horticultural Maturity Indices
the Philippines (on Mindanao Island), and New
Guinea. It has spread throughout the tropical At maturity, the fruit naturally falls from the tree
world under the general name of “durian” (Indo- at the articulation of the fruit stem with the fruit,
Malay) or variants thereof, such as “duren” then ripens in 2 to 4 days, with the fruit normally
(Indonesian), “duyin” (Burmese), “thureen” splitting into segments of irregular widths at
(Cambodian), “thurian” (Thai), “saurieng” the stylar end. Ripening results in an increase in
(Vietnamese), “dulian” (Filipino), “stinkvrucht” soluble sugars and a decrease in starch and pulp
(Dutch), and “kadu” (Sudan). Limited supplies firmness that occurs before natural fruit splitting
are available from the Caribbean and Central and starts.
South America. Commercial supplies are available
from Thailand and Malaysia. To prevent natural fruit fall, fruit may be tied
to the limb or harvested at maturity. Maturity is
judged by appearance (fruit stalk thickness and
Quality Characteristics and Criteria flexibility, abscission zone, or carpel sutures),
number of days after flowering, and a hollow
Weighing up to 8 kg (18 lb), the oval or ellipsoid, sound when tapped with a wood or rattan stick
green to brownish fruit can measure up to 30 or knife. The number of days after flowering and
cm (12 in) long and 20 cm (8 in) in diameter. It tapping are the most reliable criteria (Siriphanich
is densely covered with stout, sharp, pyramidal 1996). ‘Chanee’ durian takes 2 to 4 days, while
spines 1 cm (0.5 in) long on its thick, fibrous ‘Monthong’ durian takes 4 to 6 days to ripen after
rind. Fruit are divided into 3 to 5 smooth-walled harvest, depending on maturity. Fruit at 85%
compartments, each containing 1 to 6 glossy, maturity, based on days from anthesis and rind
creamy to red-brown seeds 2 to 6 cm (0.8 to 2.4 characteristics, ripen to excellent quality in under
in) long covered by a white to yellowish, soft, 1 week at 28 to 31 °C (82 to 88 °F). Ripening
318
takes longer than 1 week at 22 °C (72 °F). Fruit Controlled Atmosphere (CA)
that are 95% mature when harvested have already Considerations
commenced ripening, while 75% mature fruit
may ripen with an inferior quality. Compared with Ripening is inhibited by 2% O2, and fruit fail
fruit picked from the tree, fruit collected from the to ripen when removed to air. Fruit stored in up
ground after falling are more subject to disease to 20% CO2 in air were not affected in terms of
and fracture and have a shorter shelf-life (2 to 3 ripening or quality (Tongdee et al. 1990). Low
days instead of the 7 to 8 days for fruit picked O2 (10%) reduces respiration rate and ethylene
from the tree). In Thailand, the fruit is harvested production but does not affect the onset of
with the stem (peduncle) attached, and the stem ripening, and ripe fruit quality is not affected. The
is wrapped in a leaf or paper to reduce wilting aril remains hard in less mature fruit stored in 10%
and maintain the fresh appearance of the stem, O2 and 15 or 20% CO2. The commercial potential
since stem appearance is also used as a measure of of CA or MA is still unclear (Siriphanich 1996).
freshness. ‘Chanee’ is at optimum eating stage for
only a few days while ‘Mon Tong’ is at this stage
for a longer period. Retail Outlet Display Considerations
Use forced-air or room cooling to 15 °C (59 °F). The pulp of half-ripe or nearly fully ripe fruit is
much less sensitive to chilling injury than the peel,
and the pulp can be stored for 4 weeks at 5 °C
Optimum Storage Conditions (41 °F). Whole fruit stored at under 15 °C (59 °F)
develop chilling injury shown by the peel turning
This climacteric fruit, when stored at 15 ºC (59 black or dark brown starting at the groove between
ºF), has extended shelf-life (Brooncherm and the spines (Brooncherm and Siriphanich 1991).
Siriphanich 1991); RH of 85 to 95% is best (Ketsa Chilling-injured pulp suffers a loss of aroma, does
and Pangkool 1994, Sriyook et al. 1994). Fruit not soften, and may develop sunken areas on the
can be waxed to reduce water loss (Sriyook et al. surface (Siriphanich 1996).
1994). Fruit ripened at a lower RH (75%) have a
better eating quality and are less juicy and easier
to dehusk than fruit ripened at a higher RH (Ketsa Ethylene Production and Sensitivity
and Pangkool 1994).
Production of ethylene varies from near zero
in immature fruit up to 40 µL kg-1 hr-1 at the
climacteric peak and varies with cultivar (Tongdee
319
et al. 1987a,b). Most of the ethylene production Collectrichum, Fusarium, and Rhizopus can
is associated with the husk, as the pulp has a very sometimes be severe (Siriphanich 1996).
low rate (Siriphanich 1996). Durian can be ripened Sanitation, avoidance of mechanical injury, and
with ethylene gas (Ketsa and Pangkool 1995) or fungicide can be used for control (Lim 1990).
ethephon (Atantee 1995). The husk of ripened
durians will turn yellow or brown if ethylene
concentration is too high. Thai consumers prefer Quarantine Issues
to buy naturally ripened durians since the husk
remains light green or olive. Durians with a If the skin is not broken or split, durian is not
yellowish or brownish husk are not regarded as a fruit fly host. The skin must be free of other
fresh. insects such as scales.
The respiration rate for durian is 80 to 450 mg The fruit is most frequently eaten fresh. The
(45 to 254 µL) CO2 kg-1 h-1 at 22 °C. To calculate aril contains 64% water, 2.7% protein, 3.4%
heat production, multiply mg kg-1 h-1 by 220 to fat, 27.9% carbohydrate, and 23 mg per 1,000
get BTU per ton per day or by 61 to get kcal g vitamin C. Choice varieties fetch higher
per tonne per day. The peel has a much higher prices than others. Roadside and market stalls
respiration rate and ethylene production than the in Southeast Asia may cut open the fruit and
pulp (Brooncherm and Siriphanich 1991). The package the soft aril and seed in a shrink-wrapped
climacteric rise seems to occur first in the pulp. or stretch-wrapped tray. Ripe fruit and soft arils
In ‘Chanee’ and ‘Kan Yao,’ the respiration and are also frozen for export. Partially ripe fruit are
ethylene production increase during ripening but difficult to open without damaging the pulp.
decline when the fruit is overripe, while in ‘Mon
Tong,’ the climacteric peak does not occur until
the fruit is overripe (Tongdee et al. 1987b). Special Considerations
320
References Siriphanich, J. 1996. Storage and transportation
of tropical fruits: a case study on durian. In S.
Atantee, S. 1995. Effect of ethephon on ripening Vijaysegaran, M. Pauziah, M.S. Mohamed, and S.
and quality of durians. Graduate Special Problem, Ahmed Tarmizi, Proceedings of the International
Department of Horticulture, Kasetsart University, Conference on Tropical Fruits, 23-26 July 1996,
Bangkok, Thailand. Kuala Lumpur, Malaysia, vol. 1, pp. 439-451.
MARDI, Kuala Lumpur, Malaysia.
Brooncherm, P., and J. Siriphanich. 1991.
Postharvest physiology of durian pulp and husk. Sriyook, S., S. Siriatiwat, and J. Siriphanich.
Kasetsart J. (Natural Sci.) 25:119-125. 1994. Durian fruit dehiscence—water status and
ethylene. HortScience 29:1195-1198.
Hiranpradit, H., N. Lee-Ungulasatian, S.
Chandraparnik, and S. Jantigoo. 1992. Quality Tongdee, S.C., A. Chayasombat, and S.
standardization of Thai durian, Durio zibethinus Neamprem. 1987a. Effects of harvest maturity
Murr. Acta Hort. 321:695-704. on respiration, ethylene production, and
composition of internal atmospheres of Durian
Ketsa, S., and S. Pangkool. 1994. The effect of (Durio zibethinus, Murray). Proceedings of the
humidity on ripening of durians. Postharv. Biol. Durian Workshop, Bangkok, Thailand, pp. 31-36.
Technol. 4:159-165. Thailand Institute of Scientific and Technological
Research, Bangkok, Thailand.
Ketsa, S., and S. Pongkool. 2001. Effect of
maturity stages and ethylene treatment on ripening Tongdee, S.C., S. Neamprem, and A.
of durian fruits. In C. Frisina, K. Mason, and J. Chayasombat. 1987b. Control of postharvest
Faragher, eds., Proceedings of the Australasian infection of phytophthora fruit rot in durian with
Postharvest Horticulture Conference, held at fosetyl-Al and residue levels in fruit. Proceedings
Monash University, Melbourne, Australia, of the Durian Workshop, Bangkok, Thailand,
18 September to 22 September, 1995, pp. pp. 55-66. Thailand Institute of Scientific and
67-72. Department of Natural Resources Technological Research, Bangkok, Thailand.
and Environment, Institute for Horticultural
Development, Knoxfield, Victoria, Australia. Tongdee, S.C., A. Suwanagul, S. Neamprem, and
U. Bunruengsri. 1990. Effect of surface coatings
Lim, T.K. 1990. Durian—Diseases and Disorders, on weight loss and internal atmosphere of durian
pp. 60-72. Tropical Press, Kuala Lumpur, (Durio zibethinus Murray) fruit. ASEAN Food J.
Malaysia. 5:103-107.
321
Eggplant enlarge and harden. Firmness and external
glossiness are also used as harvest indicators.
Jorge H. Siller-Cepeda Overmature fruit become pithy and bitter.
322
Controlled Atmosphere (CA) absorbed by eggplants. Thus, these products
Considerations should not be placed in close proximity to
eggplants (Cantwell and Suslow 2006).
CA storage or shipping offers little benefit. Low
O2 levels (3 to 5%) delay deterioration and the
onset of decay by only a few days. Eggplant Chilling Sensitivity
tolerates up to 10% CO2, but storage life is
not extended beyond that under reduced O2. Eggplants will develop chilling injury after storage
Wrapping fruit with plastic film to create modified for 6 to 8 days at 5 °C (41 °F) (table 1). Surface
atmosphere (MA) reduces weight loss and pitting and scald are definite external symptoms
maintains firmness due to the high RH, especially (McColloch 1966). Scald refers to brown spots
on Japanese eggplant types, which have a high or areas that are first flush with the surface but
transpiration rate (Díaz Pérez 1998a). Eggplant may become sunken with time. Browning of the
fruit wrapped in high-density polyethylene flesh and seeds is a conspicuous internal symptom
(HDPE) films maintain a fresher flavor, firmness, of chilling injury, almost invariably followed by
and quality for a longer period than nonwrapped decay caused by Alternaria sp. (Ryan and Lipton
fruit (Ben-Yehoshua et al. 1985, Díaz Pérez 1979). Chilling injury is cumulative and may be
1998b). initiated in the field prior to harvest. Symptom
development can be reduced by storage in
polyethylene bags or polymeric film overwraps
Retail Outlet Display Considerations that retard water loss; however, increased decay
from Botrytis is a potential risk.
Eggplants should never be held in contact with
ice. Odor from ginger, and possibly other odor-
producing commodities such as onions, can be
0.0 °C 1 to 2 2 to 3 —
2.5 °C 4 to 5 5 to 6 5 to 6
5.0 °C 6 to 7 8 to 9 10 to 12
7.5 °C 12 12 to 14 15 to 16
_______________________________________________________
323
Respiration Rates
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate by 2.0 at 0 °C (32 °F), 1.9 at
10 °C (50 °F), and 1.8 at 20 °C (68 °F). To calculate heat production, multiply mg
kg-1 h-1 by 220 to get BTU per ton per day or by 61 to get kcal per tonne per day.
325
Endive and Escarole Grades, Sizes, and Packaging
326
Chilling Sensitivity Special Considerations
Endive and escarole are not chilling sensitive, but Endive and escarole must be handled with care
freezing at -0.1 °C (31.8 °F) must be avoided. to avoid mechanical damage and to minimize
discoloration and pathological problems.
Temperatures must be kept low and RH high to
Ethylene Production and Sensitivity prevent loss of turgor and wilting.
Physiological Disorders
Postharvest Pathology
Quarantine Issues
327
Fennel to avoid scratching the sheaths. Mechanical injury
produces rapid browning of the leaves.
Fabio Mencarelli
Fennel is harvested by hand when the plant Fennel is not chilling sensitive.
reaches a specified size by cutting the plant from
the taproot. Outer leaves are removed, and the
remaining leaves are trimmed to 10 to 15 cm (4 to Ethylene Production and Sensitivity
6 in) in length. It is harvested year-round, except
in June and July. Ethylene production is low at 0 to 2 °C (32 to 36
°F)—about 0.5 to 1.0 mL kg-1 h-1—and increases to
2.5 to 6 mL kg-1 h-1 at 20 °C (68 °F) (Mencarelli et
Grades, Sizes, and Packaging al. 1996). No data exist on sensitivity to ethylene.
328
Respiration Rates References
Temperature mg CO2 kg-1 h-1 Bianco, V.V. 1990. Il finocchio. In V.V. Bianco and
2 °C 18 to 20 F. Pimpini, eds., Orticoltura, pp. 168-183. Patron
20 °C 24 to 40 Press, Bologna, Italy.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Mencarelli, F., R. Massantini, and F. Garosi. 1996.
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and Aspetti fisiologici della postraccolta del finocchio
1.8 at 20 °C (68 °F). To calculate heat production, fresco. Procedura Giornate di Studio sulla
multiply mg kg-1 h-1 by 220 to get BTU per ton per Coltivazione del Finocchio, 3:29-30, pp. 131-136.
day or by 61 to get kcal per tonne per day. Italian Horticultural Society, Tarquinia, Viterbo,
Italy.
Postharvest Pathology
Quarantine Issues
Special Considerations
329
Fig Quality Characteristics and Criteria
Carlos H. Crisosto and Adel A. Kader Skin color and flesh firmness of fresh figs are
related to their quality and postharvest life. Flavor
Crisosto is with the Department of Plant Sciences, is influenced by stage of ripeness, and overripe
University of California, Davis, CA. Kader was figs can become undesirable due to fermentative
with the Department of Plant Sciences, University products. Other quality indices include absence
of California, Davis, CA. He is deceased. of defects (such as bird-peck, sunburn, scab, skin
break, and stem shrivel), insects, and decay.
330
Controlled Atmosphere (CA) 1.8 at 20 °C (68 °F). To calculate heat production,
Considerations multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal per tonne per day.
CA combinations of 5 to 10% O2 with 15 to
20% CO2 are effective in decay control, firmness
retention, and reduction of respiration and Physiological Disorders
ethylene production. Postharvest life at optimum
temperature and RH depends on cultivar and Extended storage in CA can result in loss of
ripeness at harvest, but ranges from 1 to 2 weeks characteristic flavor. Figs exposed to <2% O2 or
in air and 3 to 4 weeks in CA for California-grown >25% CO2 develop off flavors due to fermentative
‘Black Mission’ and ‘Calimyrna’ figs. metabolism.
Figs should be displayed at 0 to 2 ºC (32 to 36 ºF) Alternaria rot, caused by Alternaria tenuis,
and dried with an RH of 90 to 95%. appears as small, round, brown to black spots over
the fruit surface. Any cracks on the skin make the
fruit more susceptible to the rot.
Chilling Sensitivity
Black mold rot, caused by Aspergillus niger,
Figs are not chilling sensitive. appears as dark or yellowish spots in the flesh with
no external symptoms. At advanced stages the
skin and flesh turn a slightly pink color and white
Rates of Ethylene Production and mycelia with black spore masses follow.
Sensitivity
Endosepsis (soft rot), caused by Fusarium
Temperature µL kg-1 h-1 moniliforms, appears in the cavity of the fig
0 ºC 0.4 to 0.8 making the pulp soft, watery, and brown with
5 ºC 0.8 to 1.5 sometimes an offensive odor.
10 ºC 1.5 to 3.0
20 ºC 4.0 to 6.0 Souring is a preharvest problem resulting from
yeasts and bacteria carried into figs by insects,
Figs are climacteric fruit and are slightly sensitive especially vinegar flies, resulting in odors of
to ethylene action on stimulating softening and alcohol or acetic acid.
decay severity, especially if kept at 5 ºC (41 ºF) or
higher temperatures. Recommendations to reduce postharvest diseases
are the following: controlling orchard insects
to reduce fruit damage and transmission of
Respiration Rates fungi; using effective control of preharvest
diseases; enforcing strict sanitation of picking
Temperature mg CO2 kg-1 h-1 and transporting containers; supervising careful
0 ºC 4 to 8 handling to minimize abrasions, cracks, and other
5 ºC 10 to 16 physical damage; avoiding picking figs from the
10 ºC 18 to 24 ground for fresh market; and enforcing prompt
20 ºC 40 to 60 cooling to 0 ºC (32 ºF) and maintaining the cold
chain all the way to the consumer.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
331
Quarantine Issues Chossa, I. 1997. Fig. In S. Mitra, ed., Postharvest
Physiology and Storage of Tropical and
Currently, there is limited export to Canada. There Subtropical Fruits, pp. 245-268. CAB Intl.,
is no import of fresh figs into the United States. Wallingford, U.K.
Issues associated with exotic pest quarantine
addressing both imported and exported fruit Colelli, G., F.G. Mitchell, and A.A. Kader. 1991.
change rapidly. The USDA Animal and Plant Extension of postharvest life of ‘Mission’ figs by
Health Inspection Service (APHIS) issues rules CO2-enriched atmospheres. HortScience 26:1193-
regarding import requirements. APHIS provides 1195.
information to assist exporters in targeting
markets and defining what entry requirements Condit, J.J. 1947. The Fig. Chronica Botanica Co.,
a foreign country might have for fruit. In Waltham, MA.
cooperation with State plant boards, the agency
developed the database “Excerpt” to track Obenauf, G., M. Gerdts, G. Leavitt, and J. Crane.
phytosanitary requirements for each country. 1978. Commercial dried fig production. Leaflet
APHIS also provides phytosanitary inspections 21051, University of California, Division of
and certifications that declare that fruit are free Agricultural Sciences, Davis, CA.
of pests to facilitate compliance with foreign
regulatory requirements.
Special Considerations
Acknowledgments
References
333
Controlled Atmosphere (CA) multiply mg kg-1 h-1 by 220 to get BTU per ton
Considerations per day or by 61 to get kcal per tonne per day.
Respiration rates increase when sprouting begins.
Atmospheres with high CO2 (5 to 15%) are
beneficial in retarding sprout development and
decay during storage at 0 to 5 °C (32 to 41 °F). Physiological Disorders
Low O2 (0.5%) alone does not retard sprout
development of ‘California Late’ stored up to 6 Waxy breakdown is a physiological disorder that
mo at 0 °C (32 °F). Atmospheres with 15% CO2 affects garlic during later stages of growth and is
may produce a yellow, translucent discoloration often associated with periods of high temperature
on some cloves after about 6 mo (Cantwell 2006). near harvest. Early symptoms are small, light-
yellow areas in the clove flesh that darken to
yellow or amber. Later, the clove becomes
Retail Outlet Display Considerations translucent, sticky, and waxy, but the outer dry
skins are not usually affected. Waxy breakdown
Garlic should be kept cool and dry. is commonly found in stored and shipped garlic,
rarely in the field. In addition to its association
with high preharvest temperatures and sunscald,
Chilling Sensitivity low O2 and inadequate ventilation during handling
and storage may also be contributing factors.
Garlic is not chilling sensitive, and the optimum
storage temperature of -1 °C (30 °F) is just above
the freezing point of garlic. Postharvest Pathology
334
foodservice but now found in retail food stores. References
Fresh-peeled garlic cloves are packed in rigid
clear plastic containers or in plastic film liners Cantwell, M. 2000. Garlic. Recommendations
in carton boxes. The mechanical peeling process for maintaining postharvest quality. Perishables
results in broken and damaged pieces, and damage handling. At http://postharvest.ucdavis.edu/
is the major factor leading to decay and quality produce_information.
loss during storage. Storage at 0 to 5 °C (32 to 41
°F) is imperative to maintain good quality. A 2- to Hardenburg, R.E., A.E. Watada, and C.Y.
3-week storage life is expected if garlic is kept Wang. 1986. The Commercial Storage of Fruits,
at 5 °C (41 °F) or below. Storage temperatures Vegetables, and Florist and Nursery Stocks.
above 5 °C (41 °F) will result in pink and brown Agriculture Handbook 66, U.S. Department
discoloration on the damaged areas and favor root of Agriculture, Agricultural Research Service,
and sprout development. Washington, DC.
335
Ginger Precooling Conditions
Robert E. Paull and Ching Cheng Chen Forced-air or room cooling to 12 to 14 °C (54 to
57 °F) should be used.
Paull and Chen are with the Department of
Tropical Plant and Soil Sciences, University of
Hawaii at Manoa, Honolulu, HI. Optimum Storage Conditions
Desired quality characteristics include skin color, Retail Outlet Display Considerations
plumpness of tuber pieces, sheen on skin, and
absence of vegetative sprouts, blemishes, soil, Display fresh young ginger with misting and
and insect injury. Young ginger is bright yellow to mature ginger at ambient temperature with no
brown and has a high sheen with greenish-yellow misting.
vegetative buds, but no sprouts.
Chilling Sensitivity
Horticultural Maturity Indices
Mature ginger is chilling sensitive if held below
Mature ginger rhizomes are harvested when the 12 °C (54 °F). Symptoms include loss of skin
plant tops begin to wilt and die. These rhizomes color and pitting of the skin. In severe cases there
should be plump with a dry, bright yellow-brown is internal breakdown.
skin. The sheen is soon lost and the skin darkens
after harvest.
Ethylene Production and Sensitivity
336
1.8 at 20 °C (68 °F). To calculate heat production, Haware, M.P., and L.K. Joshi. 1974. Studies on
multiply mg kg-1 h-1 by 220 to get BTU per ton per soft rot of ginger from Madhya Pradesh. Indian
day or by 61 to get kcal per tonne per day. Phytopath. 27:158-161.
Fusarium rot (Fusarium spp.) can cause serious Trujillo, E.E. 1963. Fusarium yellows and rhizome
problems. Symptoms include pale-brown rot of common ginger. Phytopathology 53:1370-
discoloration of the vascular strands (Trujillo 1371.
1963) that invades the rest of the rhizome, which
then becomes brown and dry (Teakle 1965).
Pythium rot (Pythium spp.) has also been reported.
The rhizome becomes soft and watery (Haware
and Joshi 1974). Fungicides are not permitted, but
reasonable control is obtained if the rhizomes are
adequately cured and held at 12 to 14 °C (54 to 57
°F). Saprophytes, such as Penicillium spp., may
grow on cut ends and injured areas and, though
not parasitic, they give the cut ends and surface an
unsightly appearance.
Quarantine Issues
References
337
Ginseng Precooling Conditions
Seung Koo Lee and Sang Don Yun Ginseng roots are commonly hydrocooled or
forced-air cooled to below 5 ºC (41 °F). Cooling
Lee and Yun are with the Department of generally retards subsequent deterioration such as
Horticulture, Seoul National University, Suwon, weight loss and decay.
South Korea.
Optimum Storage Conditions
Scientific Name and Introduction
Ginseng should be stored at 0 ºC (32 °F) with 95%
American ginseng (Panax quinquefolium L.), RH or higher. Roots retain good quality for 2 mo
Chinese sanch’i ginseng (Panax notoginseng), at 0 ºC (Yun 1998) and 20 days at 25 ºC (77 °F)
Japanese ginseng (chikusetsu-ningin) (Panax (Oh et al. 1979).
japonica), and Korean ginseng (Panax ginseng
C.A Meyer) are all perennials of the Araliaceae
family (Bae 1978). The edible portion is the main Controlled Atmosphere (CA)
root with two to five lateral roots. Ginseng is used Considerations
as a cure-all medicine in Asia (Proctor 1990).
Ginseng is grown primarily between latitudes 30º Reduced microorganism growth and attenuated
to 48º N in Canada, China, Korea, and the United cavitation are the major benefits of CA. Optimal
States. CA is 1% O2 with >5% CO2 (Lee and Kim 1979,
Yun and Lee 1998). Cavitation is significantly
reduced at 15% CO2 (Yun 1998).
Quality Characteristics and Criteria
High-quality ginseng has a firm main root without Retail Outlet Display Considerations
defects. It should be clearly defined to show a
head (rhizome), body (main root), and legs (lateral Ginseng roots are displayed with green leafy
roots). vegetables, and water loss is controlled by
humidification or packaging.
Special Considerations
Quality Characteristics and Criteria Store and display grapes at the coldest
refrigeration temperature possible. Delays in
High-quality grapes are free of injury, decay, cooling greatly increase shatter and decay (Lutz
cracking, and sunscald; appear and feel turgid; 1939).
have a dry stem scar; and are fully colored. The
rachis should be green and berries are firmly
attached to pedicels. Bunches should be compact, Chilling Sensitivity
but berries not too tightly packed.
American grapes are not known to be chilling
sensitive.
Horticultural Maturity Indices
For fresh market, berries should be harvested Ethylene Production and Sensitivity
when soluble solids content (SSC) is 14 to 18%.
Stimulation of Botrytis cinerea (gray mold)
growth can occur on berries and stems in the
Grades, Sizes, and Packaging presence of ethylene. Ethylene production from
American grapes is less than 0.1 µL kg-1 h-1.
No standard packaging is used with American
grapes. Grapes are usually packed as intact or
trimmed clusters (rachis and berries) in bulk in
lugs, in quart-size vented plastic containers, or in
plastic slit bags of 2 lb (0.9 kg).
340
Respiration Rates Suitability as Fresh-Cut Product
Disorders encountered on American grapes Lutz, J.M. 1938. Factors Influencing the Quality
include sunburn, shrivel from low-RH storage, of American Grapes in Storage. Bull. 606, U.S.
and bleaching or stipple near berry pedicels from Department of Agriculture, Washington, DC.
SO2 application (Morris et al. 1992).
Morris, J.R., O.L. Oswald, G.L. Main, et al. 1992.
Storage of new seedless grape cultivar with sulfur
Postharvest Pathology dioxide generators. Am. J. Enol. Vitic. 43:230-
232.
American grapes are susceptible to gray mold
(Botrytis cinerea Pers.), ripe rot (Colletotrichum Hewitt, W.B. 1988. Berry rots and raisin
gloesporioides [Penz.] Penz. & Sacc.), molds. In R.C. Pearson and A.C. Goheen, eds.,
macrophoma rot (Botryosphaeria dothidea Compendium of Grape Diseases and Insects, pp.
[Moug. Ex Fr.] Ces & de Not.), powdery mildew 26-27. APS, St. Paul, MN.
(Uncinula necator [Schw.] Burr.), blue mold
(Penicillium), alternaria (Alternaria alternata
[Fr.] Keissl), and aladosporium rot (Cladosporium
herbarum Pers.:Fr.) (Hewitt 1988). Undeveloped
berries can show infection by black rot
(Guignardia bidwelli [Ellis] Viala & Ravaz).
Quarantine Issues
None.
341
Grape (Muscadine) Grades, Sizes, and Packaging
342
Ethylene Production and Sensitivity Special Considerations
Stimulation of Botrytis cinerea (gray mold) Sodium metabisulfite pads and generators (12 to
growth can occur on grapes and stems in the 21 µL L-1 SO2) have been used to extend shelf-
presence of ethylene. Ethylene production from life of muscadines from 2 to 8 weeks (Smit et al.
muscadines is less than 0.1 µL kg-1 h-1 (Perkins- 1971, Ballinger and Nesbitt 1982, James et al.
Veazie, unpublished). 1997).
Temperature mg CO2 kg-1 h-1 Ballinger, W.E., and W.B. Nesbitt. 1982. Quality
of muscadine grapes after storage with sulfur
2 °C 6 to 14
dioxide generators. J. Amer. Soc. Hort. Sci.
5 °C 8 to 18
107:827-830.
20 °C 33 to 68
Data from Perkins-Veazie (2002, unpublished). Ballinger, W.E., and W.F. McClure. 1983. The
effect of ripeness on storage quality of ‘Carlos’
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate muscadine grapes. Sci. Hort. 18:241-245.
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production, Flora, L.F. 1977. Storage stability of juices
multiply mg kg-1 h-1 by 220 to get BTU per ton per and jellies made from muscadine grapes (Vitis
day or by 61 to get kcal per tonne per day. rotundifolia Michx.). Amer. J. Enol. Vitic. 28:171-
173.
Scientific Name and Introduction Most California table grapes are packed in the
field. In contrast to South Africa and Chile, few
The table grape (Vitis vinifera L.) is a grapes are shed-packed in the United States. The
nonclimacteric fruit with a relatively low rate most common field-packing system is the “avenue
of physiological activity. It is subject to serious pack.” Fruit are picked and placed into picking
water loss following harvest, which can result in lugs. Usually, the picker also trims the cluster. The
stem drying and browning, berry shattering, and picking lug is then transferred a short distance to
even wilting and shriveling of berries. Gray mold, the packer, who works at a small, portable stand in
caused by the fungus Botrytis cinerea, requires the avenue between vineyard blocks.
constant attention and treatment during storage
and handling. In California, the major cultivars Shed-packed fruit are harvested by pickers and
are ‘Thompson Seedless’ (Sultanina) and ‘Flame placed in field lugs without trimming. The fruit
Seedless,’ marketed mostly during the summer are then placed in the shade of the vines to await
months up to 8 to 10 weeks after harvest. Present transport to the shed. At the packing shed, the field
interest centers on other introduced seedless lugs are distributed to packers who select, trim,
‘Fantasy’ cultivars such as ‘Ruby Seedless’ and and pack the fruit. Often two different grades are
‘Crimson.’ Seeded ‘Red Globe’ is becoming packed simultaneously by each packer to facilitate
important late in the season. quality selection. In some operations, trimming,
color sorting, and a first quality sorting may occur
in the field. In all of the systems, grapes are nearly
Quality Characteristics and Criteria always packed on a scale to facilitate packing to a
precise net weight, whether field- or shed-packed.
High consumer acceptance is attained for fruit In general, mid- and late-season grapes are packed
with high soluble solids content (SSC) or a high in plastic bags or wrapped in paper. For early-
ratio of SSC to total acidity (TA)—that is, SSC/TA season grapes, bulk pack is mainly used. In all
ratio. Berry firmness is also an important factor for cases, packed lugs are subject to quality inspection
consumer acceptance as are lack of defects such as and check weighing.
decay, cracked berries, stem browning, shriveling,
sunburned, dried berries, and insect damage. After packing and lidding, grapes are palletized
on disposable or recycled pallets. Some strapping
in the field before loading is necessary in grapes
packed in shoebox boxes. Often, loaded pallets
coming from the field pass through a “pallet
squeeze,” a device that straightens and tightens
the stacks of containers. These pallet loads
344
are unitized, usually by strapping or netting. Controlled Atmosphere (CA)
Some palletizing glue is used in shed-packing Considerations
operations. This glue bonds the corrugated
containers vertically on the pallet so that only CA of 2 to 5% O2 combined with 1 to 5% CO2
horizontal strapping is required. during storage or shipment is not currently
recommended for table grapes, because it is only
slightly beneficial. SO2 is used for decay control.
Precooling Conditions CO2 at 10 to 15% in air can be used to control
grey mold for 2 to 4 weeks depending on cultivar.
Cooling must start as soon as possible, and SO2
applied, within 12 h of harvest. Many forced-air
coolers for grapes in California are designed to Retail Outlet Display Considerations
achieve seven-eighths cooling in 6 h or less. After
cooling is complete, pallets are moved to a storage Use of a cold table for display is recommended.
room to await transport.
Chilling Sensitivity
Optimum Storage Conditions
Table grapes are not chilling sensitive.
Ideally, storage rooms should operate at -1 to 0 ºC
(30 to 32 ºF) with 90 to 95% RH and a moderate
airflow of 20 to 40 ft3 min-1 ton-1 (0.63 to 1.25 kL Ethylene Production and Sensitivity
min-1 metric ton-1) of stored grapes. The constant
low temperature, high RH, and moderate airflow Table grapes produce less than 0.1 µL kg-1 h-1 at 20
are important to limit water loss from fruit stems. ºC (68 ºF) ethylene. They are not very sensitive to
Fruit should be stored at a pulp temperature ethylene.
of -0.5 to 0 ºC (31 to 32 ºC) throughout their
postharvest life.
Respiration Rates
Optimum Temperature For grape clusters; that is, berries and stems:
Temperature mg CO2 kg-1 h-1
A storage temperature of -1 to 0 ºC (30 to 32 0 ºC 2 to 4
ºF) is recommended for mature fruit. Freezing 5 ºC 6 to 8
damage may occur in less mature grapes. The 10 ºC 10 to 16
highest freezing point for berries is -3.0 ºC (27 20 ºC 24 to 30
o
F), but the freezing point varies depending on
SSC. A -2 ºC (28 ºF) freezing point for stems has Stem respiration is approximately 15-fold higher
been reported for wine grapes. More recent table than berry respiration.
grape cultivars are more sensitive to stem freezing
damage. An RH of 90 to 95% and an air velocity To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
of approximately 20 to 40 ft3 min-1 (0.63 to 1.25 by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
kL min-1) is suggested during storage. 1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal per tonne per day.
345
Physiological Disorders finally masses of gray-colored spores develop.
Wounds on the berry surface near harvest provide
Shatter is a loss of berries from the cap stem. opportunities for infection, though no wound
In general, shatter increases in severity with is required for infection under wet conditions.
increasing maturity (that is, the longer fruit remain Removing desiccated, infected grapes from the
on the vine). Berries of seedless cultivars are previous season can reduce gray mold infection.
usually less well attached to the cap stem than Leaf-removal canopy management, preharvest
seeded cultivars. Shatter varies considerably from fungicides, and trimming visibly infected, split,
season to season, and there is a large variation cracked, or otherwise damaged grapes before
among varieties. Gibberellin applied at fruit set packing is recommended. Prompt cooling and
weakens berry attachment. Shatter is mainly fumigation with SO2 (100 µL L-1 for 1 h) are
due to rough handling during field packing with essential to control gray mold during cold storage.
additional shatter occurring all the way to final Because of increased interest in the export market,
retail sale. Shatter incidence can be reduced there is a need to use SO2-generating pads,
by controlling pack depth and packing density especially for long-distance export marketing in
(in3 lb-1), using cluster bagging, practicing which grapes are in ocean transport for extended
gentle handling, and maintaining recommended periods. These pads have sodium metabisulfite
temperature and RH. Cane-girdling reduces incorporated into them that releases SO2 during
shattering incidence. transit and marketing.
Waterberry is associated with fruit ripening Other pathogens. Black rot, caused by Aspergillus
and most often begins to develop shortly after niger; blue rot, caused by Penicillium spp.; and
veraison (berry softening). The earliest symptom rhizopus rot, caused by Rhizopus stolonifer or R.
is the development of small (1- to 2-mm) dark oryzae, become important at warmer temperatures,
spots on the cap stems (pedicles) and/or other and they commonly appear sometime during
parts of the cluster framework. These spots transport or marketing after grapes are removed
become necrotic and slightly sunken, and they from cold storage. They are at least partially
expand to affect more areas. The affected berries controlled by SO2 fumigation, though little
become watery, soft, and flabby when ripe. In research has been done to show this (Snowdon
California, this disorder has been associated with 1990).
a high-nitrogen-status vine, canopy shading, or
cool weather during veraison and fruit ripening. Sulfur dioxide use. Botrytis rot of grapes is not
Avoid overfertilization with nitrogen. Foliar sufficiently reduced by fast cooling alone. It is
nutrient sprays of nitrogen should be avoided in standard practice in California to fumigate with
waterberry-prone vineyards. Removing affected SO2 immediately after packing and follow with
berries during harvest and packing is a common, lower-dose treatments weekly during storage.
though labor-intensive, practice. Formulas for calculating initial and subsequent
weekly SO2 fumigation dosages using the
traditional system are available (Luvisi et al.
Postharvest Pathology 1995, Nelson 1985). It was demonstrated that
the amount of SO2 gas needed to kill Botrytis
Gray mold. The most destructive of the spores or to inactivate exposed mycelium depends
postharvest diseases of table grapes is gray on the concentration and length of time the
mold (Botrytis cinerea), primarily because it fungus is exposed to the fumigant. A cumulative
develops at temperatures as low as 31 oF (-0.5 concentration, calculated as the product of
o
C) and grows from berry to berry. Gray mold concentration and time, called “CT product,”
first turns berries brown, then loosens the describes the SO2 exposure needed to kill the
skin of the berry. Its white, threadlike hyphal decay organism. A CT of at least 100 µL L-1 h-1 is
filaments erupt through the berry surface, and the minimum required to kill spores and mycelia
346
of Botrytis at 0 oC (32 oF). This finding was the Grapes imported into the United States are
basis for the development of the total utilization fumigated with methyl bromide, following
system (Luvisi et al. 1992). Total utilization often treatment schedules issued by APHIS, to prevent
uses about half as much SO2 as the traditional entry of insect pests. Cold treatments are also
method and improves uniformity and effectiveness accepted by APHIS for the control of fruit flies.
of SO2 fumigant. In this total utilization system, Of primary concern are the vine moth, Lobesia
the first fumigation is done in conjunction with botrana; the Mediterranean fruit fly, Ceratitis
forced-air cooling. The forced air flows through capitata; and miscellaneous external-feeding
boxes and ensures good penetration of SO2 even to insects.
the center boxes on a pallet. In most combinations
of boxes and packs, this system produces over Grapes exported from the United States may
80% penetration, measured as a percentage of the harbor pests of concern elsewhere, but they rarely
room air CT product. Storage fumigation is done require treatment, though this situation can change
every 7 to 10 days. rapidly. Black widow spiders are occasional
hitchhikers within grape clusters or within grape
During ocean shipment for periods longer than boxes. SO2 fumigation, alone or combined with
10 days or long retail handling in which SO2 CO2, has been used successfully to kill spiders
fumigation cannot be applied, the use of SO2- before export. Omnivorous leafroller, Platnota
generating pads in combination with a box liner is stultana, is found on grapes in California and has
advised. These SO2-generating pads have sodium the potential to be a pest of regulatory concern on
metabisulfite incorporated into them to allow a table grapes exported to counties where this pest is
constant and slow release of SO2 during shipping not found. Two methods of control are insecticidal
and marketing. In California, a slow-release CA treatment (Ahumada et al. 1996) and low-
SO2-generating pad, combined with a perforated temperature storage combined with SO2 slow-
polyethylene box liner with 1/4-in (6.4-mm) holes release generators (Yokoyama et al. 1999).
at 3- to 4-in (7.6- to 10.2-cm) center, reduces
water loss and assures gray mold control without
enhancing SO2 phytotoxicity (Crisosto et al. Suitability as Fresh-Cut Product
1994).
Grapes are well-adapted to a stemless packaging
system.
Quarantine Issues
347
References Nelson, K.E. 1985. Harvesting and Handling
California Table Grapes for Market. DANR Pub.
Ahumada, M.H., E.J. Mitcham, and D.G. Moore. no. 1913, University of California, Oakland, CA.
1996. Postharvest quality of ‘Thompson Seedless’
grapes after insecticidal controlled-atmosphere Nelson, K.E. 1978. Precooling—its significance
treatments. HortScience 31:833-836. to the market quality of table grapes. Int. J. Refrig.
1:207-215.
Cappellini, R.A., M.J. Ceponis, and G.W.
Lightner. 1986. Disorders in table grape shipments Ryall, A.L., and J.M. Harvey. 1959. The Cold
to the New York market, 1972-1984. Plant Dis. Storage of Vinifera Table Grapes. Agriculture
70:1075-1079. Handbook 159, U.S. Department of Agriculture,
Agricultural Marketing Service, Washington, DC.
Crisosto, C.H., J.L. Smilanick, N.K. Dokoozlian,
and D.A. Luvisi. 1994. Maintaining table grape Snowdon, A.L. 1990. A Color Atlas of Postharvest
postharvest quality for long distant markets. In Diseases and Disorders of Fruits and Vegetables,
Proceedings of the International Symposium on vol. 1, General Introduction and Fruits. CRC
Table Grape Production, Anaheim, California, Press, Boca Raton, FL.
June 28-29,1994, pp. 195-199. American Society
for Enology and Viticulture, Davis, CA. Yahia, E.M., K.E. Nelson, and A.A. Kader. 1983.
Postharvest quality and storage-life of grapes as
Harvey, J.M., and W.T. Pentzer. 1960. Market influenced by adding carbon monoxide to air or
Diseases of Grapes and Other Small Fruits. controlled atmospheres. J. Amer. Soc. Hort. Sci.
Agriculture Handbook 189, U.S. Department 108:1067-1071.
of Agriculture, Agricultural Marketing Service,
Washington, DC. Yokoyama, V.Y., G.T. Miller, and C.H. Crisosto.
1999. Low temperature storage combined with
Kanellis, A.K., and K.A. Roubelakis-Angelakis. sulfur dioxide slow release pads for quarantine
1993. Grape. In G.B. Seymour et al., eds., control of omnivorous leafroller Platynota
Biochemistry of Fruit Ripening, pp. 189-234. stultana (Lepidoptera: Tortricidae). J. Econ.
Chapman and Hall, London, U.K. Entom. 92(1):235-238.
348
Grapefruit Grades, Sizes, and Packaging
In markets that emphasize processing, grapefruit Chilling injury can occur with low-temperature
must achieve a minimum juice content and storage, typically 5 °C (41 °F) or below. Chilling
SSC:TA ratio before harvest. injury is characterized by peel pitting. Pitting
associated with postharvest pitting is targeted
to areas of the peel surrounding oil glands,
whereas pitting associated with chilling injury
349
is not targeted to oil glands (Petracek et al. Oleocellosis can occur during harvest when
1995). Coating grapefruit with high-shine water excessive squeezing force is used to remove fruit
waxes reduces the incidence of chilling injury. from the stem. Grapefruit harvested in the morning,
Conditioning fruit by intermittent warming or when RH is high, are most susceptible because oil
stepwise lowering of temperature can also reduce glands are easily broken in turgid peel. Symptoms
chilling injury. of oleocellosis appear in the packinghouse or in
storage, and can be exacerbated by degreening.
Postharvest Pathology
Physiological Disorders
Postharvest pitting is a peel disorder that affects
Granulation, or section-drying, affects late-season waxed grapefruit stored at higher temperatures.
grapefruit dominantly on the stem or stylar end of Postharvest pitting can be reduced or eliminated
the segment. Granulation can be severe in larger by reducing fruit pulp temperature to 10 °C (50 °F)
fruit stored for extended durations (Burns and or less and coating fruit with highly gas-permeable
Albrigo 1998). Granulation can be avoided by coatings (Petracek et al. 1995, Florida Department
harvesting large fruit early in the season. of Citrus 1996).
350
Postharvest decay can result in significant losses Drenching harvested grapefruit with
of grapefruit. Postharvest grapefruit decays thiabendazole (TBZ) before packinghouse arrival
generally fall into two categories: those that is recommended for Diplodia, Phomopsis,
develop as a result of colonization or infection anthracnose, and Penicillium control. In addition,
on the fruit before harvest (stem end rots, application of aqueous imazalil or TBZ in the
anthracnose, and brown rot) and those that wax treatment aids in control. Minimizing
develop by inoculations made through wounds degreening time by delaying harvest will assist in
made during harvest or subsequent handling controlling stem-end rot caused by Diplodia and
(blue and green mold and sour rot). Stem end rots anthracnose. Careful harvesting and handling can
develop as latent infections on the fruit button reduce injuries that allow wound pathogens to
(calyx and disc) and begin growth through the enter grapefruit. Good sanitation of packinghouse
core after harvest. The decay develops unevenly equipment and storage areas will help control
at the stem and stylar ends resulting in wavy diseases that have no effective chemical control,
margins. Stem-end rots are a problem with such as sour rot. Generally, precooling or storing
grapefruit grown in warm humid climates such as fruit after packing at temperaturesof 10 °C (50 ºF)
Florida but are rare in Mediterranean climates. or below will help control growth of postharvest
pathogens.
Diplodia natalensis is prevalent in early-season
fruit if temperatures are high and degreening is
used. Development of Phomopsis citri is favored Quarantine Issues
during the winter months when temperatures
are low and degreening is no longer necessary. In areas infested with tropical fruit flies, cold
Alternaria citri is a less aggressive fungus that treatment is an approved quarantine treatment.
can be problematic in overmature grapefruit and However, grapefruit must first be preconditioned
those in extended storage. Often the symptoms of at 10 to 15 °C (50 to 59 °F) to increase resistance
alternaria, internal black discoloration generally to chilling injury. After 1 week, temperatures
towards the stem end, are not visible until the fruit can be reduced to 0.6 to 2.2 °C (33 to 36 °F) for
are cut. 14 to 24 days. In areas of low fly infestation, a
less stringent temperature-duration schedule can
Anthracnose, Colletotrichum gloesporioides, be used (Florida Department of Citrus 1996).
is a minor problem that can appear on late- The appearance of citrus canker (Xanthomonas
season fruit. Brown rot, caused by Phytophthora axonopodis pv. citri) has restricted movement
citrophthora, appears more frequently in mature of grapefruit grown in affected areas in Florida.
fruit and fruit stored for longer durations at low Compliance with the Citrus Canker Eradication
temperatures. Green and blue mold, caused by Program (2000) is required for harvesting, pack-
Penicillium digitatum and italicum, respectively, ing, and shipping fruit from quarantined areas.
invade fruit through wounds made during
harvest handling. Growth of P. digitatum is more
favorable at temperatures above 10 °C (50 °F), Suitability as Fresh-Cut Product
whereas growth of P. italicum occurs more readily
at lower temperatures. Immature fruit are resistant The potential for grapefruit as a fresh-cut
to sour rot (Geotrichum candidum) infection, product is great. Peeled and sectioned grapefruit
but as the fruit mature, the disease can become packaged in hard plastic containers is displayed
a problem. Consequently, late-season grapefruit on refrigerated shelves of Southeastern U.S.
can become infected, especially since the disease retail markets. Technological developments have
develops more readily at temperatures above 15 overcome various postharvest problems with
°C (59 °F) (Eckert and Brown 1986, Whiteside fresh-cut citrus. However, further development of
1988, Florida Department of Citrus 1996). automated systems for efficient and economical
peeling is essential (Pao et al. 1997).
351
References Pao, S., P.D. Petracek, and M.A Ismail. 1997.
Advances in preparing peeled fresh-cut citrus.
ASHRAE [American Society of Heating, Food Tech. Intl. 42:39-40.
Refrigerating and Air-Conditioning Engineers].
1998. Citrus fruit, bananas, and subtropical fruit. Petracek, P.D., W.F. Wardowski, and G.E. Brown.
In 1998 ASHRAE Handbook on Refrigeration. 1995. Pitting of grapefruit that resembles chilling
American Society of Heating, Refrigerating and injury. HortScience 30:1422-1426.
Air-conditioning Engineers, Atlanta, GA.
Saunt, J. 2000. Citrus Varieties of the World.
Arpaia, M.L., and A.A. Kader. 2000. Grapefruit. Sinclair Intl. Ltd., Norwich, U.K.
Recommendations for Maintaining Postharvest
Quality. At http://postharvest.ucdavis.edu/ Soule, J., and W. Grierson. 1986. Maturity and
produce_information. grade standards. In W.F. Wardowski, S. Nagy, and
W. Grierson, eds., Fresh Citrus Fruits, pp. 23-48.
Brown, G.E., P.D. Petracek, M. Chambers, et al. AVI Publishing Co., Westport, CT.
1998. Attempts to extend the market availability
of ‘Marsh’ grapefruit with storage at 2 to 3 ºC. Wardowski, W.F. 1996. Recommendations for
Proc. Fla. State Hort. Soc. 111:268-273. Degreening Florida Fresh Citrus Fruit. Circular
1170, pp. 1-3. Florida Cooperative Extension
Burns, J.K., and L.G. Albrigo. 1998. Time of Service, University of Florida, Gainesville, FL.
harvest and method of storage affect granulation
in grapefruit. HortScience 33:728-730. Whiteside, J., S. Garnsey, and L. Timmer, eds.
1988. Compendium of Citrus Diseases. APS Press,
Echeverria, E., J.K. Burns, and W.M. Miller. 1999. St. Paul, MN.
Fruit temperature and maturity affect development
of blossom end clearing in grapefruit. HortScience
34:1249-1250.
352
Greens for Cooking Grades, Sizes, and Packaging
353
baskets or boxes to help maintain low temperature senescence and leaf yellowing (Suslow and
and high RH. When properly handled, greens Cantwell 2002).
in general may be kept for about 2 weeks
(Hardenburg et al. 1986, Sanders et al. 1999,
Suslow and Cantwell 2002). Turnip greens are Respiration Rates
particularly perishable, in contrast to kale, which
has been kept in excellent condition for up to 3 The data provided below are for spinach. Similar
weeks under ideal storage conditions (Hardenburg data are not available for other types of leafy
et al. 1986). greens.
Though greens are sometimes displayed on Freezing injury, which results in water-
unrefrigerated counters, they should be placed soaked tissue and decay, is the most common
on ice or on refrigerated counters. Intermittent physiological disorder. Temperatures slightly
misting minimizes water loss and wilting. below 0 °C (32 °F) can result in freezing (Suslow
and Cantwell 2002).
Chilling Sensitivity
Postharvest Pathology
Leafy greens are not sensitive to chilling
temperatures and should be stored as cold as Bacterial soft rots, primarily caused by Erwinina
possible without freezing. and Pseudomonas, are the most common types
of postharvest decay (Suslow and Cantwell
2002). Other market diseases may be found, but
Ethylene Production and Sensitivity in general these are not of serious concern if
appropriate disease control has been implemented
The ethylene production rate for fresh spinach during production and if greens are properly
is <0.1 µL kg-1 h-1 at 20 ºC (68 °F) (Suslow and handled.
Cantwell 2002). Similar data are not available
for other greens, but their ethylene production
rates could reasonably be expected to be in the Quarantine Issues
same general range as that of spinach (Kader
1992). All leafy greens are sensitive to ethylene There are no current issues. However, anyone
in the postharvest environment, which accelerates exporting fresh produce should check with the
354
appropriate agency in the importing country to Hardenburg, R.E., A.E. Watada, and C.Y.
ensure that all regulations are met. Wang. 1986. The Commercial Storage of Fruits,
Vegetables, and Florist and Nursery Stocks.
Agriculture Handbook 66, U.S. Department
Suitability as Fresh-Cut Product of Agriculture, Agricultural Research Service,
Washington, DC.
All leafy greens have potential for fresh-cut
marketing. Hurst, William C. 2000. Commercial production
and management of cabbage and leafy greens.
University of Georgia Bull. 1181. At http://pubs.
Special Considerations caes.uga.edu/caespubs/pubcd/B1181.
Harvest workers should sanitize cutting tools Kader, A.A. 1992. Postharvest biology and
periodically to avoid the spread of decay-causing technology: an overview. In A.A. Kader, ed.,
microorganisms. Postharvest Technology of Horticultural Crops,
pp. 15-20. DANR Pub. no. 3311, University of
California, Davis, CA.
References
Maynard, D.N., and G.J. Hochmuth. 1997. Knott’s
AMS [USDA Agricultural Marketing Service]. Handbook for Vegetable Growers. 4th ed. John
1946. United States Standards for Grades of Wiley & Sons, New York, NY.
Spinach Leaves (Fresh). U.S. Department of
Agriculture, Agricultural Marketing Service, Saltveit, M.E. 1997. A summary of CA and MA
Washington, DC. requirements and recommendations for harvested
vegetables. In M.E. Saltveit, ed., 7th International
AMS [USDA Agricultural Marketing Service]. Controlled Atmosphere Research Conference,
1953a. United States Standards for Grades University of California, Davis, July 13-18, 1997,
of Collard Greens or Broccoli Greens. U.S. vol. 4, pp. 98-117. University of California Davis,
Department of Agriculture, Agricultural Marketing CA.
Service, Washington, DC.
Seelig, R.A. 1974. Collards—Fruit and Vegetable
AMS [USDA Agricultural Marketing Service]. Facts and Pointers. United Fresh Fruit and
1953b. United States Standards for Grades Vegetable Association, Alexandra, VA.
of Mustard Greens and Turnip Greens. U.S.
Department of Agriculture, Agricultural Marketing Suslow, T.V., and Marita Cantwell. 2002. Spinach,
Service, Washington, DC. recommendations for maintaining postharvest
quality. Produce Facts. University of California,
Boyhan, G.E., William C. Hurst, W. Terry Davis, CA. At http://postharvest.ucdavis.edu/
Kelley, et al. 2004. Postharvest handling and produce_information.
transportation of fruits and vegetables. University
of Georgia Fact Sheet 100. At http://pubs.caes.uga.
edu/caespubs/pubcd/FS-100.
355
Guava Grades, Sizes, and Packaging
Robert E. Paull and Ching Cheng Chen Guava fruit are commonly shipped in 4.5-kg
(10-lb) single-layer cartons with foam sleeves or
Paull and Chen are with the Department of wrapping to prevent injury.
Tropical Plant and Soil Sciences, University of
Hawaii at Manoa, Honolulu, HI.
Precooling Conditions
356
when chilled. Browning of the flesh can occur. rot (Phomopsis destructum), and rhizopus rot
Decay incidence and severity increase with (Rhizopus stolonifer). Orchard sanitation and
chilling injury. Ripe, soft fruit can be held at 5 °C effective postharvest management, such as
(41 °F), as they are less sensitive to chilling injury. avoiding mechanical injury and prompt cooling,
can reduce disease incidence.
357
Honeydew Melon white, slightly waxy, and may have very sparse
netting. Full-slip hybrid honeydew melons, versus
Gene Lester and Krista Shellie honeydew melons cut at harvest, are perceived by
consumers to have superior flavor, texture, and
Lester and Shellie are formerly with the Kika de la sweetness.
Garza Subtropical Agricultural Research Center,
Agricultural Research Service, U.S. Department
of Agriculture, Weslaco, TX. Lester is now with Horticultural Maturity Indices
the Office of National Programs, Agricultural
Research Service, U.S. Department of Honeydew melons, hybrids and nonhybrids, are
Agriculture, Beltsville, MD. Shellie is now with ready to eat when the peel turns pale green to
the Horticultural Crops Research Laboratory, cream colored and the surface feels waxy. The
Agricultural Research Service, U.S. Department blossom end gives when pressed with the thumb,
of Agriculture, Parma, ID. and the melon has a pleasant aroma. Less ripe and
cold melons have little aroma. The majority of
honeydew melons have green flesh, but specialty
Scientific Name and Introduction fruit can have gold, orange, or pink flesh.
Cucumis melo L. (Inodorus Group), called winter Casaba melons are ready to eat when the very
melons even though they are mainly grown in the furrowed or wrinkled peel is yellow and the
spring in Texas and summer elsewhere, include blossom end is springy. The flesh should be soft,
honeydew, casaba, crenshaw, and canary melons almost white, with a slight salmon cast around
(Bailey 1976). This melon group is an annual, the seed cavity and subtly sweet. No aroma is
tender, running herb of the Cucurbitaceae (gourd) produced except for a hint of cucumber.
family that is grown for its sweet, flavorful fruits
with light-green, white, or pink flesh. Honeydew Crenshaw melons are a cross between casaba
melons are the dominantly grown and shipped and persian (see “Netted Melons”). Crenshaw
melon of this group and are primarily produced in melons are ready to eat when half the dark-green
Arizona, California, and Texas. peel turns yellow, the blossom end is springy,
and a pleasant spicy aroma is emitted at room
temperature. The very sweet and juicy flesh should
Quality Characteristics and Criteria be salmon color and soft. Entirely yellow and soft
fruit are overripe and unpleasant to eat.
Minimum quality standards for honeydew melons
are good internal quality of 8% soluble solids Canary melons are ready to eat when the peel,
content (SSC) (10% in California); firm; well- generally smooth but sometimes furrowed, is
formed; mature; and free of aphid stain, rust bright canary yellow (the brighter the peel, the
spot, bruises, broken skin, solar injury (sunscald riper the melon) and the oval-shaped fruit is
and sunburn), hail damage, moisture loss, springy at the blossom end. The flesh should be
insect damage, or growth cracks (AMS 1981). crisp, flavorful, and white, with a hint of pink
Nonhybrid honeydew melons are ready for harvest around the seed cavity. A fragrant aroma is emitted
(cutting) when the rind is slightly waxy and the at room temperature.
color is mostly whitish with a light-green tinge.
These fruit should be well filled out and covered
by a fine fuzz of hairs. Superior honeydew melon Grades, Sizes, and Packaging
quality at harvest is associated with a whitish
peel, high SSC, and round fruit shape (Lester Grades include U.S. No. 1, U.S. Commercial,
and Shellie 1992). Hybrid honeydew melons will and U.S. No. 2, based primarily on percentage
abscise (slip) when mature and are mostly creamy of honeydew fruit that meet decay, damage,
and disease tolerance guidelines (AMS 1981).
358
Honeydew fruit have no Federal marketing Chilling Sensitivity
standard for SSC except for May 1 through June
20, when all honeydew melons regardless of grade Chilling injury can occur at temperatures below
must be at 8% SSC minimum (AMS 1981). Size 7 ºC (45 °F), but the riper the melon the more
classification is the number of fruit (based on a tolerant to chilling injury. Injury is expressed as
uniform fruit diameter and fruit weight) per box to pitting and darkened, elongated patchy surface
achieve a standard weight of 13.6 kg (30 lb). The lesions.
distinct size classes are 4, 5, 6, 8, and 9.
360
Bruton, B.D. 1995. Etiology, epidemiology, and
control of muskmelon fruit rots. In G.E. Lester
and J.R. Dunlap, eds., Cucurbitaceae ’94, pp. 48-
54. Gateway Printing Co., Edinburg, TX.
361
Horseradish Grades, Sizes, and Packaging
362
during storage. An increase in sucrose occurs as Postharvest Pathology
CO2 increases up to 7.5% (Weichmann 1980).
The soilborne fungus Verticilium dahliae Kleb.
infects the vascular tissue of the horseradish plant,
Retail Outlet Display Considerations resulting in discoloration (Eastburn and Weizierl
1995). The discoloration often appears as black
Washed horseradish roots packed in polyethylene specks in cross section or as streaks along the root
bags can be placed on refrigerated shelves for when the root is cut lengthwise. It is the major
several days at temperatures below 10 °C (50 °F). reason for loss in market quality of horseradish
roots.
Chilling Sensitivity
Quarantine Issues
Horseradish roots are not chilling sensitive and
can survive temperatures as low as -8 °C (18 °F) None.
(Bohling and Hansen 1980).
363
Hardenburg, R.E., A.E. Watada, and C.Y.
Wang. 1986. The Commercial Storage of Fruits,
Vegetables, and Florist and Nursery Stocks.
Agriculture Handbook 66, U.S. Department
of Agriculture, Agricultural Research Service,
Washington, DC.
364
Jerusalem Artichoke Quality Characteristics and Criteria
Stanley J. Kays Tuber size and shape are critical quality attributes
and are strongly modulated by cultivar and
Kays is with the Department of Horticulture, production conditions. Many clones have
University of Georgia, Athens, GA. an irregular tuber surface topography due to
branching, an undesirable trait.
365
and harvested as needed. Cold storage is highly Retail Outlet Display Considerations
effective, but costly. Regardless, refrigerated
storage is routinely used for seed and fresh Water loss accounts for the majority of postharvest
market tubers, especially in situations where field weight loss during retail sales. Product should be
storage is not a viable alternative. Root cellars, displayed in refrigerated display cases and, when
champs, and pits are used when the tubers must be not packaged, under high RH conditions such as
harvested in the fall, prior to the ground freezing that afforded by mist systems.
or other adverse conditions occurring, and
refrigeration is not available or is prohibitively
expensive. Chilling Sensitivity
The selection of in situ field storage depends Tubers can withstand low temperatures without
on several factors. Location is the primary damage but freeze at -2.2 °C (28 °F) (Whiteman
determinant in the potential success of in situ field 1957). Freezing at -10 °C (14 °F), whether in the
storage. Field storage is a viable option in northern field or in storage, causes rapid deterioration, but
hemisphere production areas where cold soil nonlethal freezing at -5 °C (23 °F) causes little
temperatures prevail throughout the winter, but damage. As with most fleshy plant products,
freezing of the soil surface is uncommon. Sandy, temperature at which freezing damage occurs
well-drained soils are preferred because they allow and extent of damage vary with cultivar, season,
harvest throughout the winter. Locations that do preconditioning, rate of freezing, and other factors
not meet these criteria generally require the use of (Kays 1997).
refrigeration or some form of common storage.
366
Physiological Disorders Special Considerations
Storage losses are due primarily to desiccation, During storage, tubers undergo significant
rotting, sprouting, freezing, and inulin alterations in carbohydrate chemistry, which,
degradation. Desiccation remains a significant depending on the intended use, can have a
storage problem even though losses can be pronounced effect on quality. Inulin is not one
fairly easily circumvented with proper storage compound, but a series of molecules of varying
conditions. Storage at high RH is essential chain length that begin to depolymerize during
(Shoemaker 1927, Steinbauer 1932) because storage (Jefford and Edelman 1963, Schorr-
tubers lack a corky surface layer similar to that Galindo and Guiraud 1997), whether harvested
found on potatoes to reduce water loss; their thin, or left in situ. The degree of polymerization
easily damaged surface permits rapid water loss. is critical for uses such as fat replacement or
While beneficial for some produce (Kays 1997), high-fructose syrups. With the former, as the
γ-irradiation greatly accelerates inulin degradation chain length decreases the ability of inulin
(Salunkhe 1959) and is of little storage value. to mimic a lipid diminishes. Likewise, with
progressive depolymerization, the ratio of
fructose:glucose decreases and, upon hydrolysis,
Postharvest Pathology yields a progressively less pure fructose
syrup. For example, during winter storage the
Storage rots are a serious problem (McCarter fructose:glucose ratio decreases from 11 to 3
and Kays 1984, Barloy 1988), and higher storage (Schorr-Galindo and Guiraud 1997). Thus, syrups
temperatures result in greater loss. Approximately derived from stored tubers contain a lot more
20 organisms causing storage rots have been glucose than fructose.
isolated from Jerusalem artichoke tubers. The
organisms most frequently isolated were Botrytis
cinerea Pers. and Rhizopus stolonifer (Ehrenb.: References
Fr.) Vuill., though R. stolonifer and Sclerotinia
sclerotiorum (Lib.) de Bary are the most Barloy, J. 1988. Jerusalem artichoke tuber diseases
serious organisms causing rots at low storage during storage. In G. Grassi and G. Gosse, eds.,
temperatures. Sclerotium rolfsii Sacc. and Erwinia Topinambour (Jerusalem Artichoke). Proceedings
carotovora spp. carotovora (Jones) Bergey et of a Workshop Held in Madrid, 30 September and
al., in contrast, are not significant pathogens at 1 October 1987, pp. 145-149. Office for Official
temperatures below 20 °C (68 °F). Storage rots Publications of the European Communities,
are controlled by storage at 0 to 2 °C (32 to 34 Luxembourg, Luxembourg.
°F), removal of diseased tubers, minimizing
mechanical damage, and proper RH control. Denny, F.E., N.C. Thornton, and E.M. Schroeder.
1944. The effect of carbon dioxide upon the
changes in the sugar content of certain vegetables
Quarantine Issues in cold storage. Cont. Boyce Thompson Inst.
13:295-311.
None.
Jefford, T.G., and J. Edelman. 1963. The
metabolism of fructose polymers in plants. II.
Suitability as Fresh-Cut Product Effect of temperature on the carbohydrate changes
and morphology of stored tubers of Helianthus
No current potential. tuberosus L. J. Exp. Bot. 14:56-62.
367
Kays, S.J. 1997. Postharvest Physiology of
Perishable Plant Products. Exon Press, Athens,
GA.
368
Jicama Grades, Sizes, and Packaging
369
et al. 1992, Mercado and Cantwell 1998). No Physiological Disorders
chilling injury is observed on roots stored at 12.5
°C (55 °F). Decay is the main external symptom See Chilling Sensitivity section above.
of chilling injury, and discoloration and loss of
crisp texture are the main internal symptoms.
The roots eventually become “rubbery” in texture Postharvest Pathology
when severely chilled. Internal discoloration
typically occurs from the skin inwards and is The most common decay organisms found
more common and more severe in moderately externally on jicama roots are species of
chilled roots stored at 10 °C (50 °F). At lower Penicillium, Rhizopus, and Cladosporium (Bruton
temperatures, the pulp will take on a translucent 1983, Cantwell et al. 1992). Most postharvest
appearance but not necessarily develop brown decay of jicama is a consequence of mechanical or
discoloration; these roots also exhibit external chilling injury.
decay.
Quarantine Issues
Ethylene Production and Sensitivity
None.
Jicama produces only very low amounts of
ethylene, <0.1 µL kg-1 h-1, though higher rates may
be observed after chilling at 10 °C (50 °F), about Suitability as Fresh-Cut Product
0.5 µL kg-1 h-1. Jicama is not sensitive to ethylene
exposure (Cantwell 2006). Fresh-cut jicama is incorporated in mixed
vegetable snack trays because of its crisp texture
and sweet-starchy flavor. Fresh-cut jicama
Respiration Rates should be stored below 5 °C (41 °F) to reduce
microbial growth and discoloration. A shelf-life
Temperature Intact roots Fresh-cut pieces of 4 to 8 days can be expected at 5 °C (41 °F)
----------mg CO2 kg-1 h-1---------- in air. Modified atmosphere with 5 to 10% CO2
0 °C 4 to 8 4 to 8 maintains quality and extends shelf-life of fresh-
5 °C 10 to 12 8 to 12 cut jicama (Aquino-Bolaños et al. 2000).
10 °C 9.5 to 19 11 to 19
12.5 °C 4 to 8 -
20 °C 5 to 7 - Special Considerations
Data for intact commercial size roots from
Cantwell et al. (1992). Curing. The periderm of jicama roots is easily
damaged during harvest and transport, and this
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate leads to an unsightly appearance, high rates of
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and water loss, and increased susceptibility to decay.
1.8 at 20 °C (68 °F). To calculate heat production, Wound healing or curing can be achieved by
multiply mg kg-1 h-1 by 220 to get BTU per ton per holding jicama roots at 20 to 25 °C (68 to 77 °F)
day or by 61 to get kcal per tonne per day. under 95 to 100% RH for at least 1 week. These
conditions are similar to those described for curing
At 5 °C and 10 °C, respiration rates increase sweetpotatoes and other tropical root and tuber
during storage; rates decrease during storage at crops.
temperatures above 10 °C. Less mature roots may
have higher rates (Bergsma and Brecht 1992).
Respiration rates for fresh-cut pieces were from
2-by-3 cm cylinders (Aquino-Bolaños et al. 2000).
370
References
371
Kiwifruit Horticultural Maturity Indices
372
Table 1. 1999/2000 Kiwifruit size designation chart
_____________________________________________________________________
21 22 1/2
25 27 1/2
27/28 30 1/2
30 33 1/2
33 36 3/8
36 42 3/8
39 48 3/8
42 53 3/8
45 55 1/4
_____________________________________________________________________
1
Tolerance: Average weight of all sample units must be at least 8 lb (3.63 kg) and no
individual sample unit may weigh less than 7 lb 12 oz (3.52 kg).
2
Tolerance: Not more than 10% of the containers in any lot and not more than 5%, by
count, of the fruit in any container may fail to meet the diameter range, except for sizes
42 and 45 in which the tolerance, by fruit count, may not be more than 25%.
A variety of packages are used for kiwifruit. Tray- has been used successfully but is not generally
packed fruit may be placed in cartons of wood or recommended because the hairs on the fruit
fiberboard that contain one, two, or three trays. surface tend to retain an excessive amount of
Appropriate padding should be placed between water, which can promote the growth of decay-
trays. Alternatively, volume-filled (typically 23 causing microorganisms (McDonald 1990). The
lb; 10.4 kg) or count-filled cartons are available. decision to precool remains a matter of choice for
Bagged fruit are generally placed in master the packer, and the process of curing (see Curing)
cartons, a common arrangement being a master is gaining acceptance. Lallu (1997) reports
container containing twenty 1-lb (0.45 kg) bags. that rapid precooling may exacerbate internal
At the retail level, fruit are sometimes sold in lots breakdown associated with chilling injury.
of 6 to 10 fruit per bag with no size specifications.
Mini wooden bins containing about 125 lb (56 kg)
of fruit are used for some markets (KAC 1999). Curing
Any size carton or configuration may be used
in the United States provided it is appropriately Curing occurs during the delay between harvest
labeled. and cooling and is characterized by some water
loss from fruit and drying of the stem scar. In New
Zealand, curing for at least 48 h has been reported
Precooling Conditions to reduce the incidence of Botrytis decay (Lallu et
al. 1997) and development of internal breakdown
Early literature recommends that kiwifruit should (Lallu 1997) during subsequent cold storage. In
be cooled to near 0 °C (32 °F) as soon as possible Chile, Retamales et al. (1997) reported that curing
after harvest for maximum storage potential. up to 72 h did not increase softening during cold
Forced-air cooling is preferred. Hydrocooling storage.
373
Optimum Storage Conditions Ethylene Production and Sensitivity
The recommended condition for commercial At harvest maturity, kiwifruit are low ethylene
storage of kiwifruit is 0 °C (32 °F) with 90 to 95% producers with rates of approximately 0.1 µL
RH. Fruit that are properly handled before storage kg-1 h-1 at 0 °C (32 °F) and about 0.1 to 0.5 µL
may be held in good condition for 4 to 5 mo. (See kg-1 h-1 at 20 °C (68 °F). Fruit that are ripe at less
Ethylene Production and Sensitivity). than 4 lb (1.8 kg) force produce 50 to 100 µL kg-1
h-1. Kiwifruit in storage are extremely sensitive
to the presence of ethylene and should not be
Controlled Atmosphere (CA) Conditions stored with commodities that produce significant
amounts. As little as 5 to 10 ppb (0.005 to 0.010
Kiwifruit respond favorably to CA. The potential µL L-1) ethylene in the storage atmosphere can
for benefit is excellent, with extension of accelerate softening without affecting other
storage life up to about 6 mo. The recommended ripening processes, resulting in unripe fruit that
conditions are 1 to 2% O2 with 3 to 5% CO2 at are excessively soft (McDonald 1990, Crisosto et
0 °C. Less than 1% O2 may induce off flavors, al. 1999).
and greater than 7% CO2 can cause internal
breakdown of the flesh. However, the risk of such
injuries is moderate as long as management of Respiration Rates
the atmosphere is given reasonable attention (see
Physiological Disorders). There is increasing Temperature mg CO2 kg-1 h-1
commercial use of CA during both storage and 0 ºC 3
transport. Refer to Kader (1997) for selected 4 to 5 ºC 5 to 7
references on kiwifruit CA research. 10 ºC 12
20 to 21 ºC 16 to 22
Data from Hardenburg et al. (1986).
Retail Outlet Display Considerations
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
Kiwifruit may be displayed on unrefrigerated by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
counters since it is desirable to have the fruit near 1.8 at 20 °C (68 °F). To calculate heat production,
eating ripeness at the time of sale. multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal per tonne per day.
Chilling Sensitivity
Physiological Disorders
Lallu (1997) reports that the fruit of A.
deliciosa cv. ‘Hayward’ are chilling sensitive at In storage or transit, the flesh of immature fruit
temperatures near 0 °C with symptoms appearing may soften rapidly and have a water-soaked
as a ring or zone of granular, water-soaked tissue appearance while the core remains hard and
in the outer pericarp at the stylar end of the fruit. tough (Beevers and Hopkirk 1990). A similar type
Other symptoms may include the development of of hard-core disorder also has been attributed
diffuse pitting and a dark, scaldlike appearance on to exposure of the fruit to damaging levels of
the skin. Curing alleviated symptoms of chilling ethylene in combination with CO2 of 8% or higher
injury (see Curing). The terms “low temperature (Crisosto et al. 1999).
breakdown,” “senescent breakdown,” and
“physiological pitting” all appear in the literature, Inadvertent freezing results in flesh translucency
but the differences among these disorders are (Beevers and Hopkirk 1990). If freezing injury
difficult to define in fruit stored for an extended occurs, it usually appears first at the stem end
period.
374
and progresses to the stylar end. The flesh of Quarantine Issues
damaged fruit may become somewhat yellow with
prolonged storage (Crisosto et al. 1999). There are no quarantine issues in the United
States. However, importers and exporters should
Pericarp granulation and pericarp translucency contact APHIS or other regulatory bodies
may occur independently, but both are associated in receiving countries to ensure quarantine
with long-term storage. The presence of ethylene requirements are met.
in storage may exacerbate symptoms. These
disorders may simply be a consequence of
senescence, as other causes are not well defined Suitability as Fresh-Cut Product
(Crisosto et al. 1999).
The potential does not seem good for national
The presence of ethylene in CA storage can or regional distribution of fresh-cut kiwifruit.
cause white-core inclusions, which are distinct However, some restaurants do serve fresh-cut
white patches of core tissue that are obvious in kiwifruit on salad bars and in fruit medleys, so
ripe fruit. Symptoms may develop as early as 3 there is a degree of consumer demand for the
weeks in storage, when atmospheric composition product. Agar et al. (1999) reported that fresh-cut
is favorable for development of the disorder slices of kiwifruit could be stored for 9 to 12 days
(Crisosto et al. 1999). if calcium treatments and modified atmospheres
were used. Emerging technologies in fresh-cut
processing may facilitate greater availability in
Postharvest Pathology retail markets.
375
References Kiwifruit Administrative Committee. 1999.
1999/2000 Season California Kiwifruit Packing
Agar, I.T., R. Massantini, B. Hess-Pierce, and Regulations Bulletin. Kiwifruit Administrative
A.A. Kader. 1999. Postharvest CO2 and ethylene Committee, Sacramento, California. At http://
production and quality maintenance of fresh-cut www.kiwifruit.org.
kiwifruit slices. J. Food Sci. 64:33-440.
Lallu, N. 1997. Low temperature breakdown in
AMS [USDA Agricultural Marketing Service]. kiwifruit. Acta Hort. 444:579-585.
1986. United States Standards for Grades of
Kiwifruit. Effective October 15, 1986. U.S. Lallu, N., M. Manning, and H. Pak. 1997. Best
Department of Agriculture, Agricultural Marketing curing practices for the orchard and packhouse.
Service, Washington, DC. New Zealand Kiwifruit Journal (March/April) pp.
35-37.
Beever, D.J., and G. Hopkirk. 1990. Fruit
development and physiology. In I.J. Warrington Manning, M., and D. Beever. 1992. An update
and G.C. Weston, eds., Kiwifruit: Science and on storage pitting research. NZ Kiwifruit Journal
Management, pp. 97-126. Ray Richards Publisher, (Feb.) pp. 5-7.
Auckland, New Zealand.
McDonald, B. 1990. Precooling, storage, and
Crisosto, C.H., E.J. Mitcham, and A.A. Kader. transport of kiwifruit. In I.J. Warrington and G.C.
1999. Kiwifruit. Fresh Produce Facts. University Weston, eds., Kiwifruit: Science and Management,
of California, Davis, CA. At http://postharvest. pp. 429-459. Ray Richards Publisher, Auckland,
ucdavis.edu/produce_information. New Zealand.
Ferguson, A.R. 1990a. The genus Actinidia. In Retamales, J., T. Cooper, and J. Montealegre.
I.J. Warrington and G.C. Weston, eds., Kiwifruit: 1997. Effects of cooling and curing regime on
Science and Management, pp. 15-35. Ray ethylene production and storage behavior of
Richards Publisher, Auckland, New Zealand. kiwifruit. Acta Hort. 444:567-571.
Ferguson, A.R. 1990b. Botanical nomenclature: Snowdon, A.L. 1990. Soft fruits and berry fruits.
Actinidia chinensis, Actinidia deliciosa, and In A Color Atlas of Postharvest Diseases and
Actinidia setosa. In I.J. Warrington and G.C. Disorders of Fruits and Vegetables, vol. 1, General
Weston, eds., Kiwifruit: Science and Management, Introduction and Fruits, pp. 255-266. CRC Press,
pp. 36-57. Ray Richards Publisher, Auckland, Boca Raton, FL.
New Zealand.
Testoni, A., B. Grassi, S. Quaroni, et al. 1997.
Hardenburg, R.E., A.E. Watada, and C.Y. Pitting on kiwifruit in storage caused by
Wang. 1986. The Commercial Storage of Fruits, Phialophora sp. Acta Hort. 444:751-756.
Vegetables, and Florist and Nursery Stocks.
Agriculture Handbook 66, U.S. Department
of Agriculture, Agricultural Research Service,
Washington, DC.
Grades, Sizes, and Packaging Kohlrabi have a very low ethylene production
rate of <0.1 µL kg-1 h-1 at 20 °C (68 °F) and a low
There are no grade standards for kohlrabi. Topped sensitivity to ethylene exposure.
kohlrabi is usually packaged in 11.4-kg (25-lb)
film bags, 22.7-kg (50-lb) film bags, or 11-kg (24-
377
Respiration Rates References
Temperature mg CO2 kg-1 h-1 Forney, C.F., and M.A. Jordan. 1999. Anaerobic
0 °C 10 production of methanethiol and other compounds
5 °C 16 by Brassica vegetables. HortScience 34(4):696-
10 °C 31 699.
15 °C 46
Data were adapted from Pieh (1965). Kasmire, R.E. and Marita Cantwell. 2002.
Postharvest handling systems: flower, leafy
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate and stem vegetables. At http://vric.ucdavis.edu/
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and postharvest/leaf.
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU per ton per Pieh, K. 1965. Cooling of fruits and vegetables.
day or by 61 to get kcal per tonne per day. Deut. Gartenbau 13:136-140
Quarantine Issues
Special Considerations
378
Leek They are usually packaged in 4.5-kg (10-lb)
cartons or wire-bound crates, holding ten 0.45-kg
Jennifer R. DeEll (1-lb) film bags. Some crates may be packed with
18 to 24 bunches, with a net weight of up to 13.6
DeEll is with the Ontario Ministry of Agriculture kg (30 lb).
and Food, Simcoe, Ontario, Canada.
Precooling Conditions
Scientific Name and Introduction
Hydrocooling, crushed ice, and vacuum-cooling
The leek, Allium porrum L. (syn. Allium are the most common methods to promptly cool
ampeloprasum L. var. porrum), is an onionlike harvested leeks to 0 °C (32 °F).
plant belonging to the Liliaceae family. The
edible portion consists of the elongated bases of
the foliage leaves (a false stem) and the lower Optimum Storage Conditions
parts, which are blanched white from being
underground. There are three major types of leek: Leeks can be stored for 2 to 3 mo at 0 °C (32
European leek, which develops a short and thick °F) with 95 to 100% RH. High RH is essential
pseudostem; Turkish leek, which develops a to prevent wilting. Good refrigeration retards
relatively long and thin pseudostem; and kurrat, elongation and curvature that develops in leeks
which does not produce a pseudostem. The kurrat at 10 to 21 °C (50 to 70 °F). Leeks held in
type is grown in the Mediterranean and the Middle polyethylene-lined crates remain saleable for 5
East for its leaves. to 6 weeks at 0 °C (32 °F) under crushed ice, 4
weeks at 0 °C without ice, more than 2 weeks
at 4.4 °C (40 °F), and 13 days at 10 °C (50 °F).
Quality Characteristics and Criteria Leeks stored in nonlined crates keep for 3 weeks
at 0 °C (32 °C) , 8 days at 4.4 °C (40 °F), and 1
High-quality leeks are firm and smooth, free of week at 10 °C (50 °F), with or without crushed
blemishes, and have characteristic white stems ice (Hruschka 1978). Freshly harvested leeks held
with dark-green leaves. The cut bottoms should naked in consumer-unit perforated polyethylene
be flat, because rounded bottoms may indicate bags or in nonperforated (sealed) polyethylene
prolonged storage. bags remain attractive and saleable for up to
10 weeks. They can last up to 10 weeks under
crushed ice at 0 °C (32 °F); for 10 weeks without
Horticultural Maturity Indices ice at 0 °C (32 °F); for 12 days at 10 °C (50 °F);
and 6 days at 21 °C (70 °F).
Leeks can grow as tall as 50 to 100 cm (20 to
39 in) in height and are harvested when the base
diameter reaches 2.54 cm (1 in). Controlled Atmosphere (CA)
Considerations
Chilling Sensitivity
Postharvest Pathology
Leeks are not sensitive to chilling and should be
stored as cold as possible without freezing. Most diseases that attack onions may also affect
leeks.
Physiological Disorders
References
Slight elongation and geotrophic curvature
may occur, even at 0 °C (32 °F). Leeks held in Hruschka, H.W. 1978. Storage and Shelf
polyethylene-lined crates elongate less than 1% Life of Packaged Leeks. Marketing Research
per week at 0 °C (32 °F) under crushed ice, 3% Report 1084, U.S. Department of Agriculture,
per week at 0 °C (32 °F) without ice, 13% per Agricultural Marketing Service, Washington, DC.
week at 4.4 °C (40 °F), and 22% per week at 10
°C (50 °F) (Hruschka 1978). No attempt has been Kurki, L. 1979. Leek quality changes during CA
made to establish a relation between elongation storage. Acta Hort. 93:85-90.
380
Saltveit, M.E. 1997. A summary of CA and MA
requirements and recommendations for harvested
vegetables. In M.E. Saltveit, ed., 7th International
Controlled Atmosphere Research Conference,
University of California, Davis, July 13-18, 1997,
vol. 4, pp. 98-117. University of California, Davis,
CA.
381
Lemon Grades, Sizes, and Packaging
Kenneth C. Gross and Joseph L. Smilanick Grading includes U.S. No. 1, U.S. Export No.
1, U.S. Combination, and U.S. No. 2. Common
Gross is formerly with the Food Quality packaging specifications are 40-lb (18.2-kg)
Laboratory, Henry A. Wallace Beltsville cartons, 10-lb (4.6-kg) mini-pack cartons, 8-lb
Agricultural Research Center, Agricultural (3.6-kg) consumer cartons, and 2-, 3-, and 5-lb
Research Service, U.S. Department of Agriculture, (0.9-, 1.4-, and 2.3-kg) bags. Sizes include 75, 95,
Beltsville, MD. He is now retired. Smilanick is 115, 140, 165, 200, and 235 count.
formerly with the Horticultural Crops Research
Laboratory, Agricultural Research Service, U.S.
Department of Agriculture, Fresno, CA. He is Precooling Conditions
now with the Commodity Protection & Quality
Research Unit, Agricultural Research Service, Most packing houses do not precool lemons
U.S. Department of Agriculture, Parlier, CA. because the anticipated benefit is too modest, or
they may need to degreen the fruit with ethylene,
which requires 20 °C (68 °F) pulp temperatures.
Scientific Name and Introduction
Lemons (Citrus limon L. Burman f.) are grown Optimum Storage Conditions
year round in California, the major producer.
Arizona and to a lesser extent Florida also Yellow lemons harvested when dark green have
produce a significant portion of the lemon crop. a much longer postharvest life than those picked
The primary varieties are ‘Eureka’ and ‘Lisbon.’ yellow, which must be marketed more rapidly due
Both have firm, smooth skins, juicy flesh, and few their shorter shelf-life. Lemons should be stored
seeds. The exact origin of the lemon is unknown, between 7 and 12 °C (45 and 54 °F) depending
but some have linked it to northwestern India. on the maturity-ripeness stage at harvest, season
of harvest, storage time, and production area.
They can be stored for up to 6 mo under the right
Quality Characteristics and Criteria conditions. Optimum RH is 85 to 95%. Because
lemons are chilling sensitive, they should not be
The primary quality characteristics are intensity stored for prolonged periods below 10 °C (50 °F),
and uniformity of yellow color, size, shape, though 3 to 4 weeks storage at 3 to 5 °C (37 to 41
smoothness, firmness, and freedom from decay °F), which is typical for some receivers, is usually
and defects including freezing damage, drying, tolerated without harm. Removal of ethylene
mechanical damage, rind stains, red blotch, from storage rooms can reduce senescence and
shriveling, and discoloration. Lemons should be fungal decay. It is generally recognized that proper
firm and have smooth, thin skins. Ripe lemons storage of lemons improves quality (juice content,
should have a pleasant citrus fragrance. Lemons flavor, and color).
with discolored, bruised, or wrinkled skins should
be avoided. Adequate ventilation must be maintained during
storage. Cartons should be kept off the floor to
prevent them becoming wet from condensation.
Horticultural Maturity Indices Lemons should be stored away from produce
having a strong odor. Also, decay can occur from
The generally accepted standard is a minimum skin cuts or scratches caused by rough handling.
juice content of 28 to 30% by volume, depending Lemons must be handled with care, and shipping
on the grade. containers should not be dropped on the floor. Any
382
affected product should be removed immediately decay, since lemons are sensitive to ethylene
to prevent mold from spreading. exposure. They should not be stored together with
ethylene-producing produce.
Lemons should not receive a water sprinkle or top Several disorders of lemon fruit are significant.
ice. Oleocellosis or oil spotting, a rind blemish that
involves the breaking of oil cells due to physical
stress on turgid fruits, results in the release of oil
Chilling Sensitivity that damages surrounding tissues. Not harvesting
lemons when they are turgid and careful handling
Chilling injury can be a major disorder of lemon, reduce the severity of this disorder. Peteca,
and it is therefore important not to store lemons another rind disorder, begins in white portion of
below 10 °C (50 °F). Symptoms include pitting of peel and develops sunken brown pits. It is favored
the skin (termed “peteca”), interior discoloration, by low temperatures before or after harvest,
red blotch, and loss of juice. Chilling injury oil applications in the grove, and an imbalance
severity depends on cultivar, production area, of calcium and potassium in the peel. Some
harvest time, maturity-ripeness stage at harvest, reduction in peteca is obtained by gibberelic acid
and time-temperature of postharvest handling applications to trees and by avoiding storage of
operations. Moderate to severe chilling injury is susceptible lemons below 13 °C (55 °F).
usually followed by decay.
Membrane stain, an internal disorder in which the
membranes between segments, or carpellary walls,
Ethylene Production and Sensitivity show irregular brown or black areas. It is reduced
by avoiding storage of lemons below 13 °C (55
Rates of ethylene production are generally <0.1 °F) and improving ventilation in storage.
μL kg-1 h-1 at 20 °C (68 °F). If degreening is
desired, lemons can be treated with 1 to 10 µL L-1
ethylene for 1 to 3 days at 20 to 25 °C (68 to 77
°F). However, it should be noted that this exposure
may accelerate deterioration and incidence of
383
Postharvest Pathology Quarantine Issues
There are three main postharvest pathological Most quarantine concerns for lemons address
diseases of lemon. eliminating fruit flies, such as the Caribbean,
Oriental, Mediterranean, and Mexican fruit flies.
Green mold and blue mold are caused by Heat, cold, and methyl bromide treatments are
Penicillium digitatum and P. italicum, certified for this purpose, but they all pose risk
respectively. Spores of these pathogens access the of injury to lemons. Harvest and export of fruit
fruit rind through wounds. Symptoms begin as from certified pest-free zones is another option
water-soaked areas at the fruit surface followed by to control fruit flies that eliminates risks of fruit
growth of colorless mycelia, and then sporulation. injury, and this approach has also been employed
Blue mold is more common when storage against citrus black fly and Fuller rose beetle.
temperatures are low, and it spreads from fruit to Quarantine authorities are concerned that citrus
fruit more readily than green mold. Fungicides black spot, an unsightly rind blemish caused
thiabendazole, imazalil, and sodium ortho-phenyl by Guignardia citricarpa that occurs in South
phenate are used for these diseases, and partial Africa and parts of Asia and South America, could
control can be obtained with biological control potentially become established in Mediterranean
agents and the immersion of fruit in soda ash or countries and in North America.
sodium bicarbonate. Incidence of these diseases is
reduced by careful handling to minimize wounds.
Suitability as Fresh-Cut Product
Sour rot is caused by Geotrichum citri-aurantii,
which enters lemons initially through wounds Lemon sections, prepared by both manual and
made by insects. Then infected fruit is digested by automatic processors, are distributed for use in the
the pathogens, which spread rapidly from fruit to food service industry.
fruit. Sour rot is associated with cool, wet growing
conditions. Partial control can be obtained by
immersing fruit in soda ash, sodium bicarbonate, Acknowledgments
or sodium ortho-phenyl phenate after harvest and
using minimal storage temperatures. Some of the information in this chapter is from
the Produce Marketing Association’s “Fresh
Other pathogens are occasionally troublesome in Produce Manual” and the University of California,
lemon storage, including Alternaria citri and the Davis, website “Fresh Produce Facts” at http://
stem-end rot fungi Diplodia natalensis, as well as postharvest.ucdavis.edu/produce_information.
Phomopsis citri, Botrytis cinerea, Trichoderma We express our appreciation to Laurie Houck,
spp., Sclerotinia sclerotiorum, and Phytopthora University of California, Davis, as well as to Bob
spp. Additional strategies to minimize postharvest Elliott and Chuck Orman, Sunkist Growers Fruit
decay include prompt cooling to the proper Sciences Division, for their helpful review of this
temperature range, maintaining optimum ranges chapter.
of temperature and RH; excluding ethylene during
transport and storage; using gibberelic acid before
harvest to delay senescence of the fruit after References
harvest; and providing sanitation throughout the
handling system. Arpaia, M.L., and A.A. Kader. 2001. Lemon.
Recommendations for Maintaining Postharvest
Quality. At http://postharvest.ucdavis.edu/
produce_information.
384
Eaks, I.L. 1961. Effect of temperature and holding
periods on physical and chemical characteristics
of lemon fruit. J. Food Sci. 26:593-599.
385
Lettuce Horticultural Maturity Indices
386
in 9-kg (20-lb) cartons. Bibb and greenhouse- Optimum Storage Conditions
grown lettuce are commonly packaged in 4.5-kg
(10-lb) cartons. Lettuce should be quickly cooled and maintained
as close to 0 °C (32 °F) as possible with 98
Lettuce harvested for processing is placed in large to 100% RH. Head types are better adapted
bulk bins for transportation to the precooling or to prolong storage than are the other types,
processing facility. Lettuce may be cored in the but none keep longer than 4 weeks, and about
field or at a local or regional processing facility. half that time at 5 °C (41 °F). Film liners or
At the processing facility, heads are cut, washed individual polyethylene head wraps are desirable
in cold water, and centrifuged to remove excess for attaining high RH; however they should
water. Cut lettuce is often mixed with other types be perforated or be permeable to maintain a
of lettuce or greens, shredded carrot, and red noninjurious atmosphere and to avoid 100%
cabbage to produce a salad bag mix. The mix RH on removal from storage. Lettuce is easily
may be treated with a processing aid composed damaged by freezing, so all parts of the storage
of a chlorine-containing compound and/or an room must be kept above the highest freezing
antioxidant or preservative during washing or point of lettuce, -0.2 °C (31.6 °F).
before packaging. The package is made from
special films that are selected to maintain a desired Though most lettuce is hand-harvested, some
lower O2 and higher CO2 concentration than in air. mechanical harvesters are available for product
The bags are then placed in cartons for temporary destined for processing into bag mixes. The
cold storage or for immediate shipment to market. attendant greater damage to the tissue and the
Since gas composition in bags results from a induced higher rates of respiration and water
dynamic interplay between tissue respiration loss requires greater attention to maintaining the
and film permeability, it is important to maintain optimal storage conditions of temperature and RH.
proper temperature and to know the respiratory
characteristics of the enclosed tissue.
Controlled Atmosphere (CA)
Considerations
Precooling Conditions
Lettuce, especially crisphead and fresh-cut,
Vacuum-cooling is the preferred method for respond favorably to CA (Saltveit 1997a). Levels
precooling all lettuces (Ryall and Lipton 1979, of 1 to 3% O2 at temperatures of 0 to 5 °C (32 to
Hardenburg et al. 1986). For effective vacuum- 41 °F) reduce russet spotting in susceptible lots.
cooling, containers and film wraps are perforated Intact heads do not benefit from elevated CO2,
or readily permeable to water vapor. If heads and injury—brown stain—may develop when
of lettuce are dry and warmer than 25 °C (77 lettuce is transferred from storage in >2% CO2
°F), clean water is sprinkled on them the before to air at 10 °C (50 °F) (Ke and Saltveit 1989). A
closing the cartons to aid cooling. A modification 2 to 5% O2 atmosphere maintains appearance of
called hydrovacuum reduces water loss during lettuce and inhibits pink rib and butt discoloration
cooling. Thorough precooling is essential because compared to air. Brown stain is intensified when
mechanically refrigerated trucks do not have O2 is reduced to 2 to 3%, but the effect differs by
enough cooling capacity to cool warm lettuce cultivar. If lettuce needs to be in transit overseas
during transit. Field heat retained in the densely for a month, an atmosphere of 2% CO2 and 3% O2
packed cartons can be removed by forced air is recommended because the reduction in decay
where vacuum-cooling facilities are not available, achieved by 2% CO2 outweighs the danger of
but it is much less effective. Hydrocooling is injury. Romaine and leaf lettuce appear to tolerate
effective for nonheading lettuce types but should a slightly higher CO2 level when packaged than
not be used with head lettuce since the water head lettuce. Browning is a major problem with
retained in the head fosters decay. fresh-cut lettuce, and is controlled by packaging in
387
<1% O2 and 10% CO2 (Lopez-Galvez et al. 1996, Physiological Disorders
Smyth et al. 1998). The elevated level of CO2 is
more effective at reducing browning of the cut Some of the more common disorders of head
surfaces than it is at inducing brown stain. lettuce include tipburn, russet spotting, brown
stain, and pink rib (Ryall and Lipton 1979, Saltveit
1997b). Hard heads are more susceptible to these
Retail Outlet Display Considerations disorders than firm lettuce. Tipburn is of field
origin, but occasionally increases in severity after
Lettuce should be maintained under cold harvest. Leaves with tipburn have brown, often
conditions to maximize storage and shelf- necrotic leaf margins. Russet spotting, which is
life. Periodic sprays of cold water minimize caused by exposure to ethylene and its induction
dehydration. Avoid storage with commodities that of the synthesis, accumulation, and oxidation of
produce ethylene such as apples and tomatoes. phenolic compounds at temperatures around 5 °C
All lettuces are very susceptible to water loss and (41 °F), occasionally causes serious losses. Russet
to ethylene-induced disorders, and they rapidly spots appear as dark brown, oval lesions on the
deteriorate at elevated temperatures. midribs, and on the green leaf tissue in severe
cases. It is easily controlled by making sure the
storage atmosphere is free of ethylene and that the
Chilling Sensitivity temperature is below 2 °C (36 °F).
Not chilling sensitive, but freezing at -0.2 °C (31.6 Lettuce should not be stored with ethylene-
°F) must be avoided. producing commodities such as apples,
cantaloupes, pears, and peaches. Storage in a
low-O2 atmosphere (1 to 8%) is very effective
Ethylene Production and Sensitivity in controlling russet spotting. Brown stain is
causedby exposure to >2.5% CO2 and appears
Ethylene production is very low, but exposure as large, irregularly shaped brown spots or
to ethylene can result in damage such as russet streaks mostly on the midrib. Pink rib occurs in
spotting and leaf yellowing. overmature heads stored at elevated temperatures
and appears as a diffuse pink discoloration of the
midrib; the cause of this disorder is unknown.
Respiration Rates
388
Suitability as Fresh-Cut Product Ryall, A.L., and W.J. Lipton. 1979. Handling,
Transporation and Storage of Fruits and
Suitability is very high, especially in salad mixes Vegetables, 2nd ed., vol. 1, Vegetables and
with other leafy greens. Melons. AVI Pub. Co., Westport, CT.
Some of the information in this chapter, notably Smyth, A.B., J. Song, and A.C. Cameron. 1998.
the respiration data, was taken from the University Modified atmosphere packaged cut iceberg
of California, Davis, website “Fresh Produce lettuce: Effect of temperature and O2 partial
Facts” at http://postharvest.ucdavis.edu/produce_ pressure on respiration and quality. J. Agric. Food
information and from the Produce Marketing Chem. 46:4556-4562.
Association’s “Fresh Produce Manual.”
References
389
Lime Grades, Sizes, and Packaging
High quality, mature limes have a juice content Stylar-end breakdown can be a significant problem
of 30% or higher, by volume (Arpaia and Kader with limes. Stylar-end breakdown begins as an
2000). U.S. consumers prefer a mature, green apparent breakdown of tissues at the stylar end
lime; these have a significantly longer postharvest- of the fruit. Typically the stylar end takes on a
life than those picked when yellow. In some other wet appearance. Large, mature fruit are more
countries, yellow limes are preferred because susceptible. Incidence of the disorder can be
of their higher juice content (Arpaia and Kader aggravated by high field heat and rough handling
2000). (Davenport and Campbell 1977, Malo and
Campbell 1994). Exposing limes to temperatures
below the optimum storage temperature can
result in chilling injury characterized by peel
390
pitting. Oleocellosis can develop on the peel Davenport, T.L., and C.W. Campbell. 1977. Stylar-
if hand-harvest begins early in the morning or end breakdown in ‘Tahiti’ lime: Aggravating
immediately after rainfall, when the peel is turgid. effects of field heat and fruit maturity. J. Amer.
Soc. Hort. Sci. 102:484-486.
Postharvest Pathology and Control Malo, S.E., and C.W. Campbell. 1994. The Tahiti
Lime. Fruit Crops Department Fact Sheet FC-8,
Key lime is very susceptible to stem-end rot IFAS, Florida Cooperative Extension Service,
caused by Diplodia natalensis and anthracnose University of Florida, Gainesville, FL
(Colletotricum). Stem-end rot caused by D.
natalensis, Phomopsis citri, and Alternaria citri Roy, M., C.O. Andrew, and T.H. Spreen. 1996.
are important postharvest diseases in Persian lime. Persian Limes in North America: An Economic
In addition, green and blue mold (Penicillium Analysis of Production and Marketing Channels.
digitatum and P. italicum, respectively) can enter Florida Science Source, Inc., Lake Alfred, FL.
through wounds made during harvesting and
handling and appear in storage. Careful handling Saunt, J. 2000. Citrus Varieties of the World.
to minimize mechanical damage can help reduce Sinclair International Ltd., Norwich, England,
blue and green mold. Proper sanitation of packing U.K.
line equipment and use of postharvest fungicides
reduce postharvest diseases. Wardowski, W., J. Whigham, W. Grierson, and
J. Soule. 1995. Quality Tests for Florida Citrus.
Bull. SP66, University of Florida Cooperative
Quarantine Issues Extension Service. University of Florida,
Gainesville, FL.
Appearance of citrus canker (Xanthomonas
axonopodis pv. citri) has restricted movement
of limes grown in south Florida. Compliance
with the Citrus Canker Eradication Program
(Florida Department of Agriculture and Consumer
Services 2000) is required to market limes from
quarantined areas.
References
391
Litchi Grades, Sizes, and Packaging
Robert E. Paull, Ching Cheng Chen, and Nancy There are no U.S. or international standards. One-
Jung Chen piece fiberboard boxes holding 2.25 kg (5 lb) or
4.5 kg (10 lb) with polyethylene film liners are
Paull, Chen, and Chen are with the Department of used. Fruit are also packed into 0.5-pint (0.12-L)
Tropical Plant and Soil Sciences, styrene containers.
University of Hawaii at Manoa, Honolulu, HI.
Precooling Conditions
Scientific Name and Introduction
Room-cooling is used for precooling.
Litchi (Litchi chinensis Sonn.), also spelled
lychee, originated in southern China where it
has been cultivated for at least 2,000 years. The Optimum Storage Conditions
tree has somewhat exacting requirements that
vary with cultivar for flowering, hence there Storage at 2 to 5 °C (36 to 41 °F) with 90 to 95%
is substantial year-to-year variation in supply RH should result in 3 to 5 weeks of storage life. At
(Nakasone and Paull 1998). The round to egg- 20 °C (68 °F) with 60% RH, fruit will last only 3
shaped fruit, about 2.5 cm (1 in) in diameter, has to 5 days. Fruit need to be carefully sorted before
a thin leathery red skin with blunt or sharp spines. storage to remove any damaged or decayed fruit
The edible, translucent-opaque flesh (aril) encloses or fruit with insect stings (Campbell 1959, Paull
a large (occasionally small), black seed. and Chen 1987).
392
Chilling Sensitivity Postharvest Pathology
Litchi have low sensitivity to chilling. However, Numerous postharvest diseases can occur, but
dehydration during storage often leads to loss most have their origins preharvest. Good field
of skin color and browning and is referred to as sanitation and culling of fruit that show damage
chilling injury. from fruit-piercing insects, cracks, and sunscorch
are effective in minimizing losses (Prasad and
Bilgrami 1973, Scott et al. 1982). Disease
Ethylene Production and Sensitivity organisms include Aspergillus spp. (Roth 1963,
Prasad and Bilgrami 1973, Scott et al. 1982),
Litchi have a low rate of ethylene production: <1 Pestalotiopsis spp. (Prasad and Bilgrami 1973),
nL kg-1 h-1. There are no reports on the response Peronophythora spp. (Ho et al. 1984), sour rot
of this nonclimacteric fruit to ethylene exposure. caused by Geotrichum candidum, and yeasty rots
Ethylene may lead to early aril deterioration. (Roth 1963). Other organisms found to cause rots
include Botryodiplodia theobroma, Colletotrichum
gloesporioides, and Rhizopus oryzae (Roth 1963,
Respiration Rates Prasad and Bilgrami 1973, Scott et al. 1982).
Special Considerations
Physiological Disorders
Fumigation with SO2 followed by a dip in
The major disorder is the rapid browning of the hydrochloric acid can preserve red skin color
shell from a bright red color (Paull and Chen (Paull et al. 1994). Careful application can avoid
1987, Holcroft and Mitcham 1997). The browning an increase in aril sulfite residues and avoid
is associated with water loss and injury (insect off flavors (Paull et al. 1998). Sulfites are not
stings). The browning associated with insect stings approved on fresh produce in the United States,
may go through to the pinkish-white inner surface except for grapes. Most other countries have
of the shell. A breakdown (softening and loss of sulfite residue limits for edible portions (Tongdee
turgidity) of flesh occurs in senescent fruit after 1994).
prolonged storage and overmaturity. The condition
starts at the blossom end. Field and sometimes
postharvest skin cracking can occur. Cracked fruit
should be culled.
393
References Paull, R.E., N.J. Chen, J. Deputy, et al. 1984.
Litchi growth and compositional changes during
Akamine, E.K. 1960. Preventing the darkening fruit development. J. Amer. Soc. Hort. Sci.
of fresh lichees prepared for export. Technical 109:817-821.
Progress Report 127, Hawaii Agricultural
Experiment Station, Honolulu, HI. Paull, R.E., M.E. Reyes, and M. Reyes. 1994.
Litchi and rambutan insect disinfestation:
Akamine, E.K., and T. Goo. 1973. Respiration and treatments to minimize induced pericarp
ethylene production during ontogeny of fruits. J. browning. Postharv. Biol. Technol. 6:139-148.
Amer. Soc. Hort. Sci. 98:381-383.
Paull, R.E., M.E. Reyes, and M. Reyes. 1998.
Campbell, C.W. 1959. Storage behavior of fresh Sulfite residues on litchi fruit with sulfur dioxide.
‘Brewster’ and ‘Bengal’ lychees. Proc. Florida St. Postharv. Biol. Technol. 14:229-233.
Hort. Soc. 72:356-360.
Prasad, S.S., and R.S. Bilgrami. 1973.
Ho, H.H., J.Y. Lu, and L.Y. Gong. 1984. Investigations on diseases of ‘Litchi.’ vol. 3, Fruit
Observation on asexual reproduction by rots and their control by postharvest treatments.
Peronophythora litchii. Mycologia 76:745-747. Indian Phytopath. 26:523-527.
Holcroft, D.M., and E.J. Mitcham. 1997. Roth, G. 1963. Postharvest decay of litchi fruit
Postharvest physiology and handling of litchi (Litchi chinesis Sonn.). Technical Communication,
(Litchi chinesis Sonn.). Postharv. Biol. Technol. Department of Agricultural Technical Services,
9:265-281. Pretoria, South Africa.
Joubert, A.J. 1986. Litchi. In S.P. Monselise, ed., Ragnoi, S. 1989. Controlled Atmosphere
CRC Handbook of Fruit Set and Development, pp. Storage of Fruits and Vegetables, pp. 179. CAB
233-246. CRC Press, Boca Raton, FL. International, Wallingford, U.K.
Kader, A.A. 1994. Modified and controlled Scott, K.J., B.I. Brown, G.R. Chaplin, et al. 1982.
atmosphere storage of tropical fruits. In B.R. The control of rotting and browning of litchi
Champ, E. Highley, and G.I. Johnson, eds., fruit by hot benomyl and plastic film. Sci. Hort.
Postharvest Handling of Tropical Fruits. 16:253-262.
International Conference on Postharvest Handling
of Tropical Fruits, Chiang Mai, Thailand, July Tongdee, S.C. 1994. Sulfur dioxide fumigation
1993, pp. 239-249. ACIAR Proceedings no. 50, in postharvest handling of fresh longan and
ACIAR, Canberra, Australia. lychee for export. In B.R. Champ, E. Highley,
and G.I. Johnson, eds., Postharvest Handling
Kader, A.A. 1998. Produce facts—lychee. of Tropical Fruits. International Conference on
Perishables Handling Quarterly, no. 94, University Postharvest Handling of Tropical Fruits, Chiang
of California, Davis, CA. Mai, Thailand, July 1993, pp. 186-195. ACIAR
Proceedings no. 50, ACIAR, Canberra, Australia.
Nakasone, H.Y., and R.E. Paull. 1998. Tropical
Fruits. CAB Intl., Wallingford, U.K. Vilasachandran, T., S.A. Sargent, and F. Maul.
1997. Controlled atmosphere storage shows
Paull, R.E., and J.N. Chen. 1987. Effect of potential for maintaining postharvest quality of
storage temperature and wrapping on quality fresh litchi. In A.A. Kader, ed., 7th International
characteristics of litchi fruit. Sci. Hort. 33:223- Controlled Atmosphere Research Conference,
236. University of California, Davis, July 13-18, 1997,
Abstract 54, University of California, Davis, CA.
394
Longan polystyrene containers. Fruit are clipped from
the stem, as hand removal often leads to some
Saichol Ketsa and Robert E. Paull inadvertent skin removal.
-----------Storage life-----------
Horticultural Maturity Indices Temperature Untreated SO2-treated
--------------days-----------------
Maturity is judged by shape, skin color, and flavor 0 ºC 14 to 28 21 to 42
of each cultivar. Most fruit can be picked from a 4 ºC 14 14 to 28
tree with one harvest, unless multiple flowerings 10 ºC 7 to 14 14
have occurred. No definite harvest index exists for 20 ºC 3 to 5 7
longan, but growers usually note changes in skin 30 ºC 1 to 2 3 to 5
appearance—mature fruit develop a smooth and
relatively darker skin (Wong and Ketsa 1991).
Controlled Atmosphere (CA)
Considerations
Grades, Sizes, and Packaging
No controlled atmosphere studies have been
One-piece fiberboard crates are used, holding reported, though modified atmosphere packaging
either 4.5 kg (10 lbs) or 2.25 kg (5 lbs), with in 0.03 mm (1/1000 in) polyethylene bags was
plastic liners if fruit are not already packed in tested for 7 days at room temperature, followed by
35 days at 4 °C (39 °F). A modified atmosphere
395
of 1 to 3% O2 delays browning and maintains Physiological Disorders
SSC and vitamin C content (Zhang and Quantick
1997). Treatment with 1% O2 results in a slight off Desiccation is a major problem that leads to a
flavor. rapid loss of bright yellowish color of the skin,
which turns to a dull brown (Jiang et al. 2002).
Longan fruit have a low rate of ethylene Peeled, deseeded fruit aril can be used as a fresh-
production: <1 nL kg-1 h-1. There are no reports on cut product.
ethylene sensitivity.
Special Considerations
Respiration Rates
Longan are fumigated with SO2 in Thailand and
Temperature mg CO2 kg-1 h-1 other countries to prevent skin browning and
5 °C 3.5 to 11.3 to control postharvest disease (Tongdee 1994).
10 °C 16.0 to 25.0 Though very effective, SO2 fumigation is not
20 °C 30.0 to 53.0 approved for use in the United States for fruit to
Data from Liao et al. (1983). be sold as fresh. Asian consumers prefer longan in
bunches. They assume that single fruit have fallen
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate from the bunch because it has been dropped or
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and that fruit are not fresh. Individual fruit may also
1.8 at 20 °C (68 °F). To calculate heat production, have a higher rate of weight loss.
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
396
References Tongdee, S.C. 1994. Sulfur dioxide fumigation
in postharvest handling of fresh longan and
Jiang, Y.M, Z.Q. Zhang, D.C. Joyce, and S. Ketsa. lychee for export. In B.R. Champ, E. Highley,
2002. Postharvest biology and handling of longan and G.I. Johnson, eds., Postharvest Handling
(Dimocarpus longan Lour.) fruit. Postharv. Biol. of Tropical Fruits. International Conference on
Technol. 26:241-252. Postharvest Handling of Tropical Fruits, Chiang
Mai, Thailand, July 1993, pp. 186-195. ACIAR
La-Ongsri, S., S. Gomolmanee, and O. Wara- Proceedings no. 50, ACIAR, Canberra, Australia.
Aswapati. 1993. Reducing chilling injury
symptoms in sulfur dioxide fumigated longan Wong, K.C., and S. Ketsa. 1991. Dimocarpus
fruits. In B.R. Champ, E. Highley, and G.I. longan Lour. In E.W.M. Verheij and R.E.
Johnson, eds., Postharvest Handling of Tropical Coronel, eds., Plant Resources of South-East
Fruits. International Conference on Postharvest Asia, vol. 2, Edible Fruits and Nuts, pp. 146-151.
Handling of Tropical Fruits, Chiang Mai, Pudoc Scientific Publishers, Wageningen, The
Thailand, July 1993, pp. 20. ACIAR Proceedings Netherlands.
no. 50, ACIAR, Canberra, Australia.
Zhang, D., and P.C. Quantick. 1997. Preliminary
Liao, M.L., M.S. Liu, and J.S. Yang. 1983. study on the effects of modified atmosphere
Respiration measurement of some important fruits packaging on postharvest storage of longan fruit.
in Taiwan. Acta Hort. 138:227-246. In A.A. Kader, ed., 7th International Controlled
Atmosphere Research Conference, University of
Nakasone, H.Y., and R.E. Paull. 1998. Tropical California, Davis, CA, July 1997, vol. 3, pp. 90-
Fruits. CAB Intl., Wallingford, U.K. 96. University of California, Davis, CA.
397
Longkong astringency in the flesh declines while sugars
increase six-fold (Paull et al. 1987). The skin
Robert E. Paull bruises very easily, leading to brown discoloration.
398
Controlled Atmosphere (CA) To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
Considerations by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
Fruit stored at 14 °C (58 °F) in 3% O2 and 0% multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
CO2 had 16 days of postharvest life, compared to or by 61 to get kcal tonne-1 day-1.
9 days for fruit held in air (Pantastico et al. 1975).
High CO2 aggravated postharvest skin browning,
especially at 10% O2; browning can also occur in Physiological Disorders
fruit held in polyethylene film bags. Holding in
plastic bags (0.08 mm thickness) reduces weight Abrasion and impact injury, water loss, and
loss but increases surface browning (Brown and chilling injury are the three major disorders.
Lizada 1984). Preliminary recommendations are Mechanical injury (abrasion, impact, and
5% O2 and 0% CO2 (Yahia 1998). compression) leads to skin darkening and
browning. Chilling injury symptoms are pitting
and brown scalding of the skin.
Retail Outlet Display Considerations
Chilling Sensitivity
Quarantine Issues
Chilling leads to skin browning; at 15 °C
symptoms develop after 21 days (Piyasaengthong Longkong is a fruit fly host; irradiation at 300
et al. 1997). grays has potential for disinfestation.
400
Loquat Grades, Sizes, and Packaging
401
Respiration Rates Special Considerations
Respiration rates of loquat are influenced by Loquats must be handled with care to avoid
temperature and decrease rapidly over the first mechanical damage, and low-temperature storage
4 days of storage. By the fourth day of storage, is essential for extending postharvest life.
respiration rates of stored fruit were—
Quarantine Issues
402
Luffa Controlled Atmosphere (CA)
Considerations
Stephen Morris and Jenny Jobling
There is no published information on CA for luffa.
Morris and Jobling are with the Sydney
Postharvest Laboratory, Food Science Australia,
North Ryde, New South Wales, Australia. Retail Outlet Display Considerations
Luffa are harvested immature and selected based To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
on size. by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
Optimum Storage Conditions or by 61 to get kcal tonne-1 day-1.
403
Physiological Disorders Zong, R., M.I. Cantwell, L.L. Morris, and V.
Rubatzky. 1992. Postharvest studies on four fruit-
Luffas should be handled with care; damage type Chinese vegetables. Acta Hort. 318:345-354.
to longitudinal ribs leads to water loss and
decay. Fruit are susceptible to dehydration and
toughening of the peel.
Postharvest Pathology
Quarantine Issues
Special Considerations
References
404
Mandarin (Tangerine) Grades, Sizes, and Packing
405
Degreening Postharvest Pathology
Some mandarin-growing areas, such as Florida, Stem-end rot (Diplodia natalensis and Phomopsis
have persistent high temperatures that prevent citri) is a significant problem on mandarin,
natural color break in the peel. In these cases, especially where degreening is required in early
ethylene is used to degreen (cause the destruction season fruit. Stem-end rots develop as latent
of chlorophyll) early-season mandarin. Ethylene infections on the fruit button (calyx and disc)
is used at 1 to 5 μL L-1 at 28 to 29 °C (82 to 84 before harvest and begin to grow through the core
°F) with 95% RH. Duration of ethylene exposure after harvest. Decay develops unevenly at the stem
ranges from 12 h to 3 days. One complete air and stylar ends, resulting in uneven margins.
change per hour should enter the degreening
room to avoid buildup of CO2, which can Anthracnose, caused by Colletotrichum
inhibit ethylene action, and to assist in uniform gloesporiodes, is a major decay of mandarin.
temperature and ethylene distribution (Wardowski Characterized by brown peel lesions, anthracnose
1996). appears on early-season mandarin that have
undergone lengthy degreening periods.
406
Quarantine Issues Grierson, W. 1986. Physiological disorders. In
W.F. Wardowski, S. Nagy, and W. Grierson, eds.,
The appearance of citrus canker (Xanthomonas Fresh Citrus Fruits, pp. 361-378. AVI, Westport,
axonopodis pv. citri) has restricted movement CT.
of mandarin grown in affected areas in Florida.
Compliance with the Citrus Canker Eradication Saunt, J. 2000. Citrus Varieties of the World.
Program (Florida Department of Agriculture Sinclair International, Norwich, England.
and Consumer Services 2000) is required for
harvesting, packing, and shipping mandarin from Wardowski, W.F. 1996. Recommendations for
quarantined areas to domestic markets. degreening Florida fresh citrus fruit. Circular
1170, University of Florida, Institute of Food and
Cold treatment is an approved quarantine Agricultural Science, Gainesville, FL.
treatment for citrus grown in areas infested with
tropical fruit flies. In Florida, the Caribbean Whiteside, J.O., S.M. Garnsey, and L.W. Timmer,
fruit fly (Anastrepha suspensa) may be found eds. 1988. Compendium of Citrus Diseases. APS
in citrus groves during late spring and summer. Press, St. Paul, MN.
Cold treatment involves storage of fruit below
2 ºC (36 °F) for specified periods to ensure their
freedom from fly infestation. However, because
of susceptibility to chilling injury, fruit may be
stored at higher temperatures of 10 to 15 °C (50 to
59 °F) for about 1 week prior to cold treatment to
increase resistance to chilling injury.
References
407
Mango Grades, Sizes, and Packaging
Robert E. Paull and Ching Cheng Chen There are no U.S. or international grade standards.
Fruit are sold in 16-kg (35-lb) cartons as well as
Paull and Chen are with the Department of 6-kg (14-lb) flat single-layer cartons and 4.5-kg
Tropical Plant and Soil Sciences, University of (10-lb) single-piece fiberboard boxes with various
Hawaii at Manoa, Honolulu, HI. counts.
Mango (Mangifera indica L.) is cultivated Fruit are normally forced-air cooled or room-
throughout the tropics and warmer subtropics. Of cooled, preferably within 24 h of harvest (Mattern
the numerous varieties, Florida’s ‘Tommy Atkins,’ et al. 1972).
‘Kent,’ ‘Keitt,’ and ‘Haden’ are the most common
in the United States. The fruit’s skin is yellow or
green with a golden to red blush, depending on Optimum Storage Conditions
the variety. Fruit can be round, oval, or kidney
shaped. Some varieties have a turpentine-like Storage at 10 to 13 °C (50 to 55 °F) with 85 to
smell and taste. 90% RH should give a shelf-life of 14 to 28 days
for mature green fruit, depending on variety.
Ripe fruit can be stored at 7 to 8 °C (45 to 46 °F).
Quality Characteristics and Criteria Diseases are the principal factor limiting storage
life. Optimum ripening temperature is 20 to 23 °C
Skin coloration, size, appropriate shape for (68 to 73 °F) for best appearance, palatability, and
the variety, appearance, freedom from defects decay control (Jobin-Decor 1988).
and decay, absence of fiber in the flesh, and
a turpentine-like flavor are the most common
quality parameters. Wilted, grayish discoloration Controlled Atmosphere (CA)
and pitting are undesirable. Some fruit varieties Considerations
(‘Haden’) have pinhead-sized black spotting that
is not regarded as a defect. Different cultivars show various responses to CA.
The optimum storage atmosphere for prolonging
storage and shipping is 3 to 5% O2 and 5 to 10%
Horticultural Maturity Indices CO2 at 7 to 9 °C (45 to 48 °F) with 90% RH
(Yahia 1998). Ripening delays are minor and may
The general criteria for maturity of most cultivars not be economic in all situations. Polyethylene
of mango are (1) the fruit “shoulders” have risen or other film bags, with and without an ethylene-
above the stem-end and (2) there is a slight skin absorber, give some delay in ripening. However,
color break on the first fruit of a crop. Early fruit some film bags can also result in off flavor and
from a single flowering should only be harvested abnormal skin coloration.
after a slight skin color change; 2 weeks later all
full-sized fruit can be harvested, even if there is
no apparent change in skin color. Other indices Retail Outlet Display Considerations
include SSC and TA, specific gravity, and days
from blooming. These indices depend on cultivar Mangoes can be displayed at store temperature
and season (Hatton et al. 1965, Kanes et al. 1982). and should not be misted. Bruised and diseased
fruit should be removed from display.
408
Chilling Sensitivity higher than those of unheated fruit for 4 to 6 days
(Mitcham and McDonald 1993).
Chilling susceptibility varies with cultivar;
‘Haden’ and ‘Keitt’ are particularly susceptible.
Most cultivars show injury below 10 °C (50 °F), Physiological Disorders
especially if fruit have just reached maturity.
Tolerance to chilling increases during ripening Some disorders, such as chilling injury and high
(Medlicott et al. 1990). The symptoms include CO2 injury, are induced after harvest, while
grayish, scaldlike discoloration on the skin, others are inherent. Inherent disorders occur
followed by pitting, uneven ripening, and poor intermittently and are unpredictable; for example,
flavor and color development (Hatton et al. 1965, jelly seed, which results in watery, translucent
Medlicott et al. 1990). Heat treatment prior to tissue around the seed giving an over-ripe
storage reduces injury in ‘Keitt’ (McCollum et al. appearance. It does not develop after harvest
1993). unless it was present at harvest (Young and Miner
1961). Some cultivars are very susceptible, such
as ‘Tommy Atkins’ (Lelyveld and Smith 1979).
Ethylene Production and Sensitivity Soft nose and internal breakdown (or spongy
tissue) are other disorders (Lim and Khoo 1985),
Mangoes have moderate ethylene production: though it is possible these are the same condition.
1 to 2 µL kg-1 h-1 at 20 °C (68 °F). Ethylene Sap burn is a major problem with some cultivars
induces faster and more uniform softening (O’Hare 1994), such as ‘Kensington,’ while ‘Irvin’
(Lakshminarayana 1973, Barmore 1974). Ethylene is less susceptible (Loney et al. 1992); washing
treatment can be done prior to shipping (Barmore with water and detergent helps avoid damage
and Mitchell 1977). There is disagreement in the (Brown et al. 1986).
literature regarding effect of ethylene treatment
on quality (Chaplin 1988). This may relate to
maturity when treated. Treatment of immature Postharvest Pathology
fruit leads to softening, but the fruit have poor
flavor. Anthracnose (Colletotrichum gloesporioides),
which is caused by preharvest infection and does
not spread postharvest, and the postharvest stem-
Respiration Rates end rots caused by several fungi that infect before
and after harvest (often as wound invaders that
Temperature mg CO2 kg-1 h-1 spread postharvest) are the two most common
4.5 °C 10 to 22 diseases (Johnson and Coates 1993). Anthracnose
10 °C 23 to 46 appears as fruit ripen and first appears as
15 °C 45 to 90 superficial black spots and streaks that then
20 °C 75 to 151 become sunken (Fitzell and Peak 1984).
Data from Karmarkar and Joshi (1941) and Lam
(1987). Alternaria rot (Alternaria alternata), a preharvest
infection, can sometimes be a problem, while the
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate postharvest wound infections can occasionally be
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and severe, such as black mold (Aspergillus spp.) and
1.8 at 20 °C (68 °F). To calculate heat production, transit rot (Rhizopus spp.). Disease control begins
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 in the field followed by postharvest sanitation,
or by 61 to get kcal tonne-1 day-1. as well as avoidance of latex burn (stain) and
mechanical injury. Hot-water treatment (46 °C
Heating for insect disinfestation elevates for 60 to 120 min) and fungicides can be used,
respiration 3- to 5-fold; after cooling, rates remain depending on the cultivar (Spalding and Reeder
409
1986). Hot-water brushing at 55 °C (131 ºF) for Jobin-Decor, M.P. 1988. Mango ripening guide.
20 s shows good control (Prusky et al. 1999). Queensland Agric. J. 114:369-371.
Fresh-cut pieces and slices are frequently found in Lakshminarayana, S. 1973. Respiration and
markets. Browning of the flesh can be a problem. ripening patterns in the life cycle of the mango
fruit. J. Hort. Sci. 48:227-233.
Barmore, C.R., and E.F. Mitchell. 1977. Ethylene Lam, P.F. 1987. Respiration rates and ethylene
pre-ripening of mangoes prior to shipment. Citrus production of ripening Harumanis mangoes after
Industry 58:18-19, 22-23. different chilling storage periods. MARDI Res.
Bull. 95:15-19.
Brown, B.I., I.A. Wells, and C.F. Murray. 1986.
Factors affecting the incidence and severity of Lim, T.K., and K.C. Khoo. 1985. Diseases and
mango sap-burn and its control. ASEAN Food J. Disorders of Mango in Malaysia. Tropical Press,
2:127-132. Kuala Lumpur, Maylasia.
Chaplin, G.R. 1988. Advances in postharvest Loney, B.R., S.P. Robinson, J.J. Brophy, and E.K.
physiology of mango. Acta Hort. 231:639-648. Chacko. 1992. Mango sap-burn, components of
the fruit sap and their role in causing skin damage.
Fitzell, R.D., and C.M. Peak. 1984. The Austral. J. Plant Physiol. 19:449-457.
epidemiology of anthracnose disease of mango:
inoculum sources, spore production and disposal. Mattern, F., W. Pennock, and L.S. Valles. 1972.
Ann. Appl. Biol. 104:53-59. Supplying the New York market with high quality
Puerto Rican mangoes. J. Agric. Univ. Puerto Rico
Hatton, T.T. Jr., W.F. Reeder, and C.W. Campbell. 56:1-10.
1965. Ripening and Storage of Florida Mangos.
Marketing Research Report 725, U.S. Department McCollum, T.G., S. D’Aquino, and R.E.
of Agriculture, Washington, DC. McDonald. 1993. Heat treatment inhibits mango
chilling injury. HortScience 28:197-198.
410
Medlicott, A.P., J.M.M. Sigrist, and O. Sy. 1990.
Ripening of mangoes following low temperature
storage. J. Amer. Soc. Hort. Sci. 115:430-434.
411
Mangosteen fruit skin damaged by thrips prior to harvest a
more attractive appearance.
Robert E. Paull and Saichol Ketsa
Paull is with the Department of Tropical Plant and Horticultural Maturity Indices
Soil Sciences, University of Hawaii at Manoa,
Honolulu, HI; Ketsa is with the Department of Skin color is the major criterion used to judge
Horticulture, Kasetsart University, Bangkok, maturity. Immature fruit that have a light,
Thailand. greenish-yellow skin with scattered pinkish
spots do not ripen to full flavor if harvested. The
minimum harvest stage for high quality fruit is
Scientific Name and Introduction when the skin has distinct irregular, pink-red
spots over the whole surface. Fruit are at the
Mangosteen (Garcinia mangostana L.) is edible, ripe stage when the skin has darkened to
one of the most praised of tropical fruit. It is reddish-purple, no latex remains in the skin, and
also known as mangostanier, mangoustanier, the flesh segments separate easily from the skin
mangouste, mangostier (French), mangostan (Tongdee and Suwanagal 1989). Careful handling
(Spanish), manggis, mesetor, semetah, sementah is essential in order to avoid mechanical injury.
(Malaysian), manggustan, mangis, mangostan
(Philippine), mongkhut (Cambodian), mangkhut
(Thai), cay mang cut (Vietnamese), manggis, Grades, Sizes, and Packaging
manggistan (Dutch), and mangostao, mangosta,
or mangusta (Portuguese) (Nakasone and Paull There are no U.S. or international standards. Fruit
1998). are graded by size and color. Mangosteens are
normally sold in single-layer, fiberboard cartons
The globe-shaped, smooth berry is 4 to 7 cm (1.6 holding 2.25 kg (5 lb) with padding, or sometimes
to 2.8 in) across and has a persistent calyx. The in trays with fruit individually wrapped to prevent
pericarp is 6 to 10 mm (0.24 to 0.4 in) thick and injury (20 to 24 fruit per tray). In Southeast Asia,
turns purple during ripening. It contains a bitter, fruit are sold in baskets or strung in bundles of 10
yellowish latex and purple-staining juice. The to 25 fruit.
edible white aril has 4 to 8 segments with 1 or 2
larger segments containing apomictic seeds; there
is no true seed. Precooling Conditions
412
injury. Application of surface coatings reduces Physiological Disorders
weight loss and prevents calyx wilting (Choehom
1997). Fruit damage during harvesting and marketing can
affect >20% of fruit. The “gamboges” disorder
occurs where latex seeps into the flesh (aril),
Controlled Atmosphere (CA) turning it yellow and giving it a bitter taste.
Considerations “Gamboges” also moves onto the outer surface
of the fruit. This is a preharvest disorder of
An atmosphere of 5% O2 and 5% CO2 has been unknown cause that makes it difficult to separate
used for 1 mo (Yahia 1998) and resulted in best the aril from the surrounding tissue, even in ripe
overall retention of peel appearance and internal fruit; it causes hardening of the pericarp. This
quality (Rattanachinnakorn et al. 1996). Holding should not be confused with impact injury that
fruit in polyethylene film bags reduces weight leads to hardening of the pericarp at the point
loss and disease (Daryono and Sabari 1986); of impact and aril collapse, dehydration, pink
however, it is not clear if the effects are due to color development, or browning (Tongdee and
the prevention of water loss or to the modified Suwanagul 1989). A drop of 10 cm can cause
atmosphere in the bags. slight pericarp damage, indicated as hardening
at the point of impact within 24 h. Higher drops
causing significantly greater damage often lead to
Retail Outlet Display Considerations downgrading of the fruit (Tongdee and Suwanagul
1989, Ketsa and Atantee 1998).
Fruit are commonly displayed in overwrapped
trays or in closed styrene, clam-shell containers Another disorder of mangosteen fruit is
with no perforations at 10 to 14 °C (50 to 57 °F). translucent aril (nue-kaew), believed to be
They should not be misted. induced by heavy rain during fruit growth
and development, even if just before harvest
(Laywisakul 1994). The specific gravity of fruit
Chilling Sensitivity with translucent aril is >1.0, while that of normal
aril is <1.0. This allows separation of fruit by
Storage at <10 °C (50 °F) leads to rapid hardening floating them in water (Podee 1998). Fruit with
and darkening of pericarp when fruit are returned translucent aril have lower SSC and TA than
to ambient temperature (Uthairatanakij and Ketsa normal fruit (Pankasemsuk et al. 1996).
1996, Choehom 1997).
Postharvest Pathology
Ethylene Production and Sensitivity
Botryodiplodia theobromae, Diplodia spp.,
Mangosteen is a climacteric fruit. Ethylene Pestalotia flagisettula, Phomopsis spp., and
production is about 29 nL kg-1 h-1. The respiratory Rhizopus spp. have been reported; they harden the
peak occurs sooner when fruit are treated with skin and decay the aril.
ethylene. Ethylene treatment triggers autocatalytic
ethylene production (Noichinda 1992).
Quarantine Issues
413
Suitability as Fresh-Cut Product Pantastico, E.B., ed. 1975. Postharvest Physiology,
Handling and Utilization of Tropical and
Some potential exists. Subtropical Fruits and Vegetables. AVI, Westport,
CT.
Choehom, R. 1997. Effect of waxing and plant Rattanachinnakorn, B., J. Phumhiran, and S.S.
growth regulators on quality and storage-life Nanthachai. 1996. Controlled atmosphere storage
of mangosteen (Garcinia mangostana L.) fruit of mangosteen. In Annual Technical Conference
during cold storage. Graduate Special Problem, and Proceedings of the Horticulture Research
Department of Horticulture, Kasetsart University, Institute, Department of Agriculture, Bangkok,
Bangkok, Thailand. Thailand.
Daryono, M., and S. Sabari. 1986. The practical Tongdee, S.C., and A. Suwanagul. 1989.
method of harvest time on mangosteen fruit and Postharvest mechanical damage in mangosteen.
its characteristics in storage. Bull. Penelitian Hort. ASEAN Food J. 4:151-155.
Indonesia 14(2):38-44.
Uthairatanakij, A., and S. Ketsa. 1995. Physico-
Ketsa, S., and S. Atantee. 1998. Phenolics, lignin, chemical changes of pericarp of mangosteen
peroxidase activity and increased firmness of fruits after low temperature storage. In S.
damaged pericarp of mangosteen fruit after Vijaysegaran, M. Pauziah, M.S. Mohamed, and
impact. Postharv. Biol. Technol. 14:117-124. S. Ahmad Tarmizi, eds., Proceedings of the
International Conference on Tropical Fruits, vol.
Laywisakul, S. 1994. Factors Influencing 1, pp. 411-422. Malaysian Agricultural Research
the Development of Translucent Disorder in and Development Institute, Serdang, Salangor,
Mangosteen. M.S. thesis, Kasetsart University, Maylasia.
Bangkok, Thailand.
Yahia, E.M. 1998. Modified and controlled
Nakasone, H.Y., and R.E. Paull. 1998. Tropical atmospheres for tropical fruits. Hort. Rev. 22:123-
Fruits. CAB Intl., Wallingford, U.K. 183.
414
Mushroom cleanliness, and trim quality. Mushrooms are
packed in trays or cartons with a perforated
Franciszek Adamicki polyethylene film overwrap (Suslow and Cantwell
1998).
Adamicki is with the Department of Vegetable
Storage, Research Institute of Vegetable Crops,
Skiemiewice, Poland. Precooling Conditions
415
yellowing of the cap surface. Levels of O2 <1% Physiological Disorders
can favor growth of Clostridium botulinum and
the development of off odors and off flavors, as Low storage temperatures are needed to reduce
well as cap opening and stipe elongation. For this continued development of mushrooms after
reason, CA is not commonly used (Carrier 1995). harvest. Common disorders include upward
bending of caps and opening of the veil.
Mushrooms are easily bruised by rough handling
Retail Outlet Display Considerations and develop brown discolored tissue.
416
Salunkhe, D.K., and S.S. Kadam. 1998. Handbook
of Vegetable Science and Technology. Marcel
Dekker, New York, NY.
417
Nectarine of flesh firmness are considered ready to eat.
Fruit with <27 N (6 lb-force) firmness are highly
Carlos H. Crisosto and Adel A. Kader accepted by consumers.
418
of the white-flesh and “tree ripe” nectarines are Optimum Temperature
packed into 1-layer (tray) boxes.
The optimum storage temperature is -1 to 0 °C (30
Limited volumes of partially-ripe to ripe to 32 °F). The freezing point varies, depending on
nectarines are “ranch packed” at the point of SSC, from -3 to -1.5 °C (27 to 30 °F). A RH of 90
production. In a typical “tree ripe” operation, fruit to 95% with an air velocity of approximately 50
are picked into buckets or totes that are carried by ft3 min-1 is suggested during storage.
trailer to the packing area. Packers work directly
from buckets to select, grade, size, and pack fruit
into plastic trays. Controlled Atmosphere (CA)
Considerations
419
Ethylene Production and Sensitivity cultivars, IB symptoms develop faster and more
intensely when fruit are stored at temperatures
In the following table, the lower end of the range between about 2 and 8 °C (36 and 46 °F) than
is for mature but unripe fruit; higher values are for when stored at 0 °C (32 °F) or below. At the
ripe fruit: shipping point, fruit should therefore be cooled
and held near or below 0 °C (32 °F) if possible.
Temperature µL C2H4 kg-1 h-1 During transportation if IB-susceptible cultivars
0 ºC 0.01 to 5 are exposed to approximately 5 °C (41 °F), it
5 ºC 0.02 to 10 can significantly reduce their postharvest life.
10 ºC 0.05 to 50 Several treatments to delay and limit development
20 ºC 0.10 to 160 of this disorder have been tested. Among them,
preripening fruit before storage is a successful
In general, nectarines harvested at the “well- commercially used treatment in the United States.
mature” or riper stages will ripen properly without Success of CA (10% CO2 and 10% O2) depends on
exogenous ethylene application. In some cultivars, cultivar, market life, and shipping time.
exposure to 100 µL L-1 ethylene results in more
uniform ripening of nectarines picked at the “U.S. Inking (black staining) is a cosmetic problem
mature” stage. affecting only the skin of nectarines. It is
characterized by black or brown spots or stripes.
These symptoms appear generally 24 to 48 h after
Respiration Rates harvest. Inking is a result of abrasion damage
in combination with contamination by heavy
Temperature mg CO2 kg-1 h-1 metals (iron, copper, and aluminum). This usually
0 °C 4 to 6 occurs during harvesting and hauling, though it
10 °C 16 to 24 may occur during postharvest handling. Careful
20 °C 64 to 110 fruit handling, short hauling, avoiding any foliar
nutrient sprays within 15 days before harvest, and
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate following suggested preharvest fungicide spray
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and guidelines are recommended to reduce inking.
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1. Postharvest Pathology
420
Rhizopus rot is caused by Rhizopus stolonifer and Suitability as Fresh-Cut Product
can occur in ripe or near-ripe fruit at 20 to 25 °C
(68 to 77 °F). Cooling fruit and keeping them at The optimal ripeness for preparing fresh-cut
<5 °C (41 °F) is effective against this fungus. nectarines slices is the partially ripe (>27 to 49
N, >6 to 11 lb-force) or ripe (>13 to 27 N, >3 to 6
lb-force) flesh firmness stages. These slices can be
Quarantine Issues kept at 0 °C (32 °F) with 90 to 95% RH for 2 to
12 days, depending on cultivar and ripeness, while
Because some insects such as Conotracherlus retaining good eating quality.
nenuphar (plum curculio), Cydia pomonella
(codling moth), Rhagoletis pommonella (apple
maggot), and Tetranychus pacificus (Pacific spider Special Considerations
mite) are not present in some import markets,
phytosanitary restrictions have been established. Because nectarines are a climacteric fruit, they are
Issues with exotic pest quarantine, addressing harvested at a minimum or higher maturity but
imported and exported nectarines, can change are not fully ripe; that is, they are not ready to eat.
rapidly. APHIS issues rules regarding import Nectarines must be ripened before consumption to
requirements and provide information to assist satisfy consumers.
exporters in targeting markets and defining what
entry requirements a particular country might have
for nectarines. APHIS, in cooperation with the Acknowledgments
State plant boards, developed a database called
“Excerpt” to track phytosanitary requirements Some of the information in this chapter is from the
for each country. APHIS provides phytosanitary University of California, Davis, website “Produce
inspections and certifications that declare Facts” at http://postharvest.ucdavis.edu/produce_
nectarines free of pests to facilitate compliance information.
with foreign regulatory requirements.
For nectarines, there are three main ways to deal Further Reading
with these phytosanitary requirements: inspection
prior to shipment (including use of screened crates Ceponis, M.J., R.A. Cappellini, J.M. Wells, and
transported in sealed containers), methyl bromide G.W. Lightner. 1987. Disorders in plum, peach
fumigation, and a systems approach. and nectarine shipments to the New York market,
1972-1985. Plant Dis. 71:947-952.
A phytosanitary certificate is required to import
California nectarines into Taiwan. Nectarines must Crisosto, C.H. 1994. Stone fruit maturity indices:
be free of Anarsia ineatella (peach twig borer), a descriptive review. Postharv. News Info.
Conotracherlus nenuphar (plum curculio), Cydia 5(6):65N-68N.
pomonella (codling moth), Erwinia amylovora
(fire bright), Rhagoletis pomonella (apple Crisosto, C.H., F.G. Mitchell, and S. Johnson.
maggot), Tetranychus pacificus (Pacific spider 1995. Factors in fresh market stone fruit quality.
mite), and Ceratitis capitata (Mediterranean fruit Postharv. News Info. 6(2):17N-21N.
fly). If these conditions cannot be met, then fruit
must be treated with an appropriate treatment Crisosto, C.H., F.G. Mitchell, and Z. Ju. 1999.
prior to shipment. Details of the treatment must be Susceptibility to chilling injury of peach,
recorded on the phytosanitary certificate. nectarine, and plum cultivars grown in California.
HortScience 34:1116-1118.
421
Kader, A.A., and F.G. Mitchell. 1989. Postharvest
physiology. In J.H. LaRue and R.S. Johnson,
eds., Peaches, Plums, Nectarines: Growing and
Handling for Fresh Market, pp. 154-164. Pub. no.
3331, University of California, Davis, CA.
422
Netted Melon larger, about 6 lb (2.7 kg) each, and have a bright,
orange-pink flesh.
Krista C. Shellie and Gene Lester
Shellie and Lester are formerly with the Kika de la Quality Characteristics and Criteria
Garza Subtropical Agricultural Research Center,
Agricultural Research Service, U.S. Department To meet U.S. grade standards, melons must
of Agriculture, Weslaco, TX. Shellie is now with have sufficient maturity to ensure completion of
the Horticultural Crops Research Laboratory, ripening, sufficient firmness (not soft or wilted),
Agricultural Research Service, U.S. Department shape and netting characteristic for their type,
of Agriculture, Parma, ID; Lester is now with a stem scar not wet and slippery (wet slip), no
the Office of National Programs, Agricultural sunscald (solar injury), flesh and rind free of
Research Service, U.S. Department of Agriculture, decay by fungi or bacteria, and absence of damage
Beltsville, MD. (USDA 1981). Damage includes liquid in the seed
cavity, hail injury, surface mold, aphid honeydew,
scars, cracks, ground spot rind disorders, bruises,
Scientific Name and Introduction and mechanical damage. A minimum SSC of 11%
and 9% is required for U.S. grades Fancy and U.S.
Cucumis melo L. (Reticulatus group), commonly No. 1, respectively. State market-order quality
called cantaloupe or muskmelon, is a member standards may exceed Federal grade requirements.
of the Cucurbitaceae family (Bailey et al. 1976).
True cantaloupes, members of the Cantaloupensis
group, are nonnetted fruit common to Cantaluppi, Horticultural Maturity Indices
Italy, and are seldom grown in the United States.
Western Shipper melons (grown in Arizona, Stem separation and background rind color are
California, and Texas) are grown principally used to indicate acceptable maturity for harvest.
for domestic and export markets, while Eastern As netted melons begin to ripen, a separation
Choice melons (grown in the eastern United layer, or abscission zone, develops at the point
States) are more perishable and are used where the stem attaches to the fruit. Most netted
principally for local consumption. A heavy, melons are commercially harvested when half of
uniform, tan-colored net and bright orange flesh the stem has separated from the melon (referred
characterizes the external appearance of Western to as half-slip) (Kasmire et al. 1970). Abscission
Shipper and Eastern Choice melons. Eastern zone development often corresponds to a change
Choice melons are often deeply sutured, while from green to yellow in rind background color.
Western Shipper melons usually lack sutures. If picked at proper maturity, netted melons will
continue to soften and become more aromatic after
Charentais, galia, ananas, and Persian melons harvest (Shellie 1995, Ayub et al. 1996). Netted
are not commonly grown in the United States, melons harvested prematurely by cutting the
but are gaining popularity as specialty melons. stem prior to abscission-zone development may
The French Charentais is a round, sparsely netted produce little aroma, have low SSC, and do not
melon with a grey-green rind having pronounced properly ripen (Lyons et al. 1962).
dark-green longitudinal tracts; it has a sweet,
highly-scented, orange flesh. Galia melons from
Israel are characterized by a fine, uniform net, Grades, Sizes, and Packaging
round shape, and green flesh (Karchi and Govers
1977). Ananas melons have a sparse, cracked net U.S. Grades for cantaloupes (USDA 1981) include
and white, very sweet flesh. The shape and netting U.S. Fancy, U.S. No. 1, U.S. Commercial, and
of Persian melons are similar to those of Western U.S. No. 2. The difference among grades reflects
Shipper type melons, but Persian melons are levels of tolerance for quality criteria. A minimum
423
SSC of 11% is required for U.S. Fancy and 9% Chilling Sensitivity
for U.S. No 1. There are six common size classes
(9, 12, 15, 18, 23, and 30) based on the number Sensitivity decreases as fruit mature. Full-slip,
of fruit of uniform size and weight that fit into a netted melons may be stored for 5 to 14 days at
standard 40-lb (18-kg) cardboard shipping box. 0 to 2 °C (32 to 36 °F). Less mature melons may
be damaged by storage at <2 °C (36 °F). Injury
symptoms include pitting, surface decay, and
Precooling Conditions failure to ripen (Wang 1990).
Respiration Rates
Controlled Atmosphere (CA)
Considerations Netted melons have a moderate rate of respiration
(Kader 1992).
CA in transit or storage has some potential for
extending shelf-life. The recommended level of O2 Temperature mg CO2 kg-1 h-1
is 3 to 5% for reducing respiration and ethylene 0 °C 5 to 6
production. The recommended level of CO2 is 10 4 to 5 °C 9 to 10
to 20% for reducing loss of sugar and inhibiting 10 °C 14 to 16
surface mold. Storage in an atmosphere containing 15 to 16 °C 34 to 39
>10% CO2 may result in a carbonated taste that is 20 to 21 °C 45 to 65
lost during subsequent storage in air. Off flavors, 25 to 27 °C 62 to 71
odors, and impaired ripening may develop if Data from Sholz et al. (1963).
netted melons are stored in <1% O2 or >20% CO2
(Kader 1992, Saltveit 1997). To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
Retail Outlet Display Considerations multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
The shelf-life of netted melons can be maximized
by storage under refrigeration and by avoiding
postharvest exposure to ethylene. Physiological Disorders
425
Ayub, R., M. Guis, M. Ben Amor, et al. 1996. Shellie, K.C. 1995. Enhancing ripening
Expression of ACC oxidase antisense gene characteristics of ‘Netted, Orange-flesh’ and
inhibits ripening of cantaloupe melon fruits. Nat. ‘Smooth, Green-flesh’ type melons (Cucumis
Biotech. 14:862-866. melo L.). In G.E. Lester and J.R. Dunlap, eds.,
Cucurbitacae ’94: Evaluation and Enhancement
Bailey, L.H., and E.Z. Bailey. 1976. Hortus Third. of Cucurbit Germplasm, pp. 101-103. Gateway
Macmillan, New York, NY. Printing, Edinburg, TX.
Kader, A.A., ed. 1992. Postharvest Technology of Shellie, K.C., and M.E. Saltveit. 1993. The lack of
Horticultural Crops, 2nd ed., p. 192. DANR Pub. a respiratory rise in muskmelon fruit ripening on
no. 3311, University of California, Oakland, CA. the plant challenges the definition of climacteric
behaviour. J. Exp. Bot. 44:1403-1406.
Karchi, Z., and A. Govers. 1977. Galia melon—a
new F1 hybrid for export and the local market. Scholz, E.W., H.B. Johnson, and W.R. Buford.
Hassadeh 57:630-634 (In Hebrew; English 1963. Heat evolution rates of some Texas-grown
Summary). fruits and vegetables. J. Rio Grande Valley Hort.
Soc. 17:170-175.
Kasmire, R.F., L. Rappaport, and D. May. 1970.
Effects of 2-chloroethylphosphonic acid on Snowdon, A. 1992. Color Atlas of Postharvest
ripening of cantaloupes. J. Amer. Soc. Hort. Sci. Diseases and Disorders of Fruits and Vegetables.
95:134-137. CRC Press, Boca Raton FL.
Lester, G.E., and B.D. Bruton. 1986. Relationship USDA [U.S. Department of Agriculture].
of netted muskmelon fruit water loss to 1981. United States Standards for Grades of
postharvest storage-life. J. Amer. Soc. Hot. Sci. Cantaloupes. U.S. Department of Agriculture,
111:727-731. Washington, DC.
Lyons, J.M., W.B. McGlasson, and H.K Pratt. Wang, C.Y. 1990. Chilling Injury of Horticultural
1962. Ethylene production, respiration, and Crops. CRC Press, Boca Raton FL.
internal gas concentrations in cantaloupe fruits at
various stages of maturity. Plant Physiol. 37:31- Zitter, T.A, D.L. Hopkins, and C.E. Thomas. 1996.
36. Compendium of Cucurbit Diseases. American
Phytopathological Society, St. Paul MN.
Ryall, A.L., and W.J. Lipton. 1979. Handling,
Transportation, and Storage of Fruits and
Vegetables, vol. 1. AVI Pub. Co., Westport CT.
426
Nopalitos Grades, Sizes, and Packaging
Cactus stems (“nopalitos” in Spanish) are the Nopalitos should be cooled to about 5 °C (41 °F)
rapidly growing succulent stems (cladodes) of to reduce loss of visual appearance (shiny surface)
the prickly pear cactus (Opuntia spp.). They are that results from water loss. They are usually
a warm-season vegetable but are available year- room-cooled, but also may be forced-air cooled.
round, being grown in California as a specialty Hydrocooling should be avoided as it favors
vegetable or imported from Mexico where they discoloration in damaged areas (especially where
are a traditional vegetable. Though the young spines have penetrated the surface) and decay.
stems of many Opuntia species can be eaten, most
commercial plantings of nopalitos are from O.
ficus-indica and O. inermis. Nopalitos are mostly Optimum Storage Conditions
water (92%) and carbohydrates, as well as fiber
(4 to 6%), and a little protein (1 to 2%). They also Good quality can be maintained for 3 weeks at 5
contain some minerals, principally calcium (1%), °C (41 °F) and 2 weeks at 10 °C (50 °F) with 95
and moderate amounts of vitamin C and vitamin to 99% RH (Cantwell et al. 1992). Major factors
A. They are similar in composition to dark-green limiting storage life of nopalitos are decay and
leaf lettuces (Rodríguez-Felix and Cantwell 1988). dehydration (Cantwell, 1995). Nopalitos stored
under ambient conditions rapidly lose their
brilliant shiny appearance, become dull green
Quality Characteristics and Criteria and may begin to yellow and curve inward due to
water loss. Some discoloration (chilling injury)
Good-quality nopalitos are fresh, turgid, and occurs if stored longer than 3 weeks at 5 °C (41
a brilliant green color. In the early stages of °F) (Cantwell et al. 1992).
growth, vestigial true leaves, usually subtended by
spines, are present on the stems. The leaves often
abscise by the time nopalitos reach commercial Controlled Atmosphere (CA)
size; however, green true leaves on the stem are Considerations
an additional indication of freshness. Nopalitos
should be harvested when young and tender, but No information is available on the potential
not early in the morning so as to avoid a high acid benefits of CA or MAP for nopalitos. For
content (see Special Considerations). diced product, 5 to 10% CO2 may be beneficial
(Cantwell, 2002, unpublished).
Nopalitos are chilling sensitive when stored below Decay at the cut stem end may be a problem if
10 °C (50 °F). Chilling damage may be manifested nopalitos are stored for more than 2 weeks. Decay
as a superficial bronzing or discoloration and can be avoided by insuring that nopalitos are not
increased susceptibility to decay. Symptoms may damaged when cut from the plant. Fungicide dips
appear after 3 weeks at 5 °C (41 °F) (Cantwell et reduce postharvest decay of nopalitos but are not
al. 1992) or sooner (Ramayo-Ramirez et al. 1978). used commercially (Ramayo-Ramírez et al. 1978).
Ethylene production rates are very low: 0.05, 0.1 None are known.
and 0.22 nL kg-1 h-1 at 5, 10 and 20 °C (41, 50,
and 68 °F), respectively (Cantwell et al. 1992).
Nopalitos are not sensitive to ethylene exposure, Suitability as Fresh-Cut Product
but exposure at warm temperatures will enhance
yellowing. Some types of nopalitos are spiny, and therefore a
cleaned and diced product is an attractive option.
Reducing brown discoloration at cut surfaces
Respiration Rates and preventing fluid (mucilage) loss are the main
problems in handling diced cactus stems. Cut
The following are average rates for 10-cm (4-in) nopalitos cannot be washed before marketing,
nopalitos over a 7-day period at the indicated because washing will cause mucilage to exude
temperatures. Initial rates are about 50% higher. and enhance discoloration of cut surfaces.
Respiration rates of 20-cm (8-in) stems are about Notwithstanding the chilling sensitivity of intact
50% lower than rates of 10-cm (4-in) stems. nopalitos, they should be stored between 0 and 5
°C (32 and 41 °F) (Cantwell, 2002, unpublished).
Temperature mg CO2 kg-1 h-1 Shelf-life of diced nopalitos was 1 day at 20 °C
5 °C 16 to 19 (68 °F) and 6 days at 5 °C (41 °F) (Rodríguez-
10 °C 38 to 42 Felix and Soto-Valdez 1992). Moderate CO2 (5 to
15 °C 52 to 59 10%) CA may be useful to reduce discoloration
20 °C 68 to 79 and other visual defects of cut nopalitos (Cantwell,
2002, unpublished).
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production, Special Considerations
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1. Cactus stems should be harvested and handled
with care to avoid mechanical damage, especially
from spines on one stem penetrating the
Physiological Disorders neighboring stem. Spine damage leads to a rusty-
brown discoloration and pathological problems
See above section on Chilling Sensitivity.
Because the prickly pear plant is a crassulacean
acid metabolism (CAM) plant and fixes CO2 at
night as malic acid before converting it to sugars
during the day, the acid content of nopalitos
may fluctuate greatly and affect their flavor
428
(Rodríguez-Felix and Cantwell 1988). Therefore,
it is recommended that stems be harvested 2 to 3
h after sunrise. Small nopalitos, however, are not
CAM-active. Also, low temperature storage at 5
°C (41 °F) maintains acid levels, while warmer
storage conditions of 15 to 20 °C (59 to 68 °F)
result in decreased acid (Cantwell et al. 1992).
References
429
Okra Quality Characteristics and Criteria
430
including 5 to 10% CO2 at 5 to 8 °C (41 to 46 °F) To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
and 3 to 5% O2 and 0% CO2 (Baxter and Waters by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1986). Levels of CO2 >20% can cause off flavors. 1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
Retail Outlet Display Conditions
431
Duzyaman, E. 1997. Okra: botany and
horticulture. Hort. Rev. 29:41-72.
432
Olive [lenticels] through a straw color) indicate when
to harvest. An olive is considered mature if it
Carlos H. Crisosto and Adel A. Kader exudes a characteristic white juice when squeezed.
For black olives, skin color and removal force
Crisosto is with the Department of Plant Sciences, are used; fruit reach this stage 3 to 4 mo after the
University of California, Davis, CA. Kader was green stage.
with the Department of Plant Sciences, University
of California, Davis, CA. He is deceased.
Grades, Sizes, and Packaging
For green olives, criteria are color and freedom Optimum Storage Conditions
from mechanical damage, shriveling, surface
blemishes, scale and other insect injury, and decay. Olives should be stored at 5 to 7.5 °C (41 to 46
These are processed according to California black- °F) with 90 to 95% RH. Temperatures <5 °C
ripe style or Spanish green style fermented olives. (41ºF) cause chilling injury of fresh olives.
For black olives, criteria are color, freedom from
defects, and oil content of 12 to 25% (depending
on cultivar). These are processed (Greek or Italian Controlled Atmosphere (CA)
style) or used for oil extraction. Considerations
433
(46 ºF). This information is for fresh green olives. Physiological Disorders
Fresh black olives should be processed as soon
after harvest as possible, but if necessary black Nailhead is characterized by surface pitting and
olives can be kept in 2% O2 at 5 °C (41 °F) for up spotting. It results from death and collapse of
to 4 weeks. epidermal cells, creating air pockets underneath
the skin. Symptoms are observed on olives kept
at 10 °C (50 °F) for >6 weeks or at 7.5 °C (46 °F)
Chilling Sensitivity for >12 weeks. CO2 injury is evidenced by internal
browning and increased incidence and severity of
Olives are sensitive to temperatures <5 °C (41 °F). decay. It results from exposure to >5% CO2 for
Symptoms in ‘Ascolano,’ ‘Manzanillo,’ ‘Mission,’ more than 4 weeks.
and ‘Sevillano’ fruit are a slight, tannish to brown
discoloration which develops in the flesh adjacent
to the pit. Over time, the discoloration becomes Postharvest Pathology
more intense and progresses through the flesh
into the skin, at which time the olive has the Postharvest diseases occur if olives have been
appearance of having been boiled. chilled at temperatures below 5 °C (41 °F),
mechanically damaged, not cooled promptly after
Chilling injury becomes visible on olives stored harvest to 5 to 7.5 °C (41 to 46 °F), or exposed to
for >2 weeks at 0 °C (32 °F), 5 weeks at 2 °C undesirable atmospheres (>5% CO2 or <2% O2).
(35 °F), or 6 weeks at 3 °C (38 °F). The order
of susceptibility to chilling injury, from most
to least susceptible, is ‘Sevillano,’ ‘Ascolano,’ Quarantine Issues
‘Manzanillo,’ and ‘Mission.’
Since olive fruit fly (Bactrocera oleae) is present
in many California olive-growing areas, a limited
Ethylene Production and Sensitivity amount of fresh olives is imported from Mexico
and Argentina. A very limited amount is exported
Green olives produce <0.1 µL kg-1 h-1 ethylene, to Canada. These exported and imported fresh
and black olives produce 0.5 µL kg-1 h-1 at 20 °C olives are not fumigated with methyl bromide.
(68ºF). Though olives produce very little ethylene,
they are moderately sensitive to ethylene at Issues associated with exotic pest quarantines
concentrations >1 µL L-1, which causes a loss of addressing either imported or exported fresh olives
green color and flesh firmness. can change rapidly. APHIS issues rules regarding
import requirements. This agency provides
information to assist exporters in targeting markets
Respiration Rates and defining what entry requirements a particular
country might have for fresh olives. APHIS, in
Temperature mg CO2 kg-1 h-1 cooperation with the State plant boards, developed
5 °C 10 to 20 a database called “Excerpt” to track phytosanitary
7.5 °C 16 to 24 requirements for each country. APHIS provides
10 °C 24 to 32 phytosanitary inspections and certifications that
20 °C 40 to 80 declare fresh olives free of pests to facilitate
compliance with foreign regulatory requirements.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
434
Suitability as Fresh-Cut Product Maxie, E.C., P.B. Catlin, and H.T. Hartmann.
1960. Respiration and ripening of olive fruits.
Fresh olives are not edible or suitable as a fresh- Proc. Amer. Soc. Hort. Sci. 75:275-291.
cut product.
Olias, J.M., and J.M. Garcia. 1997. Olive. In S.
Mitra, ed., Postharvest Physiology and Storage
Special Considerations of Tropical and Subtropical Fruits, pp. 229-243.
CAB Intl., Wallingford, U.K.
Olives for pickling are harvested either unripe, in
which case they remain green, or ripe, when they
are purple and turn black during pickling. Olives
for oil extraction can be harvested from the straw-
color stage through the black-ripe stage.
Acknowledgments
Further Reading
435
Onion days before lifting, bulbs can be undercut by
a blade; such onions should not be used for
Franciszek Adamicki long-term storage. Onions for bunching can be
harvested from the time they are pencil-sized until
Adamicki is with the Department of Vegetable they have proper bulb size.
Storage, Research Institute of Vegetable Crops,
Skiemiewice, Poland.
Grades, Sizes, and Packaging
Scientific Name and Introduction Grades for green onions include U.S. No. 1 and
U.S. No. 2 based on external appearance and
Onions (Allium cepa L., Cepa group) is a biennial size. For U.S. No. 1, the overall length (excluding
of the Alliaceae family. The edible portions of roots) must be 20 to 61 cm (8 to 24 in), and the
the bulb are the enlarged leaf bases and compact diameter of the bulb 6.4 to 25.4 mm (0.25 to 1 in).
stem. Green onions, also called scallions, are Sizes are based on bulb diameter: Small, <12.7
eaten for their immature bulb and green foliage. mm (0.5 in); Medium, 12.7 to 25.4 mm (0.5 to 1
The predominant flavor component results in); and Large, >25.4 mm (>1 in). Trimmed green
from activity of the enzyme alliinase in broken onions are bunched and marketed as bulb-type in
or crushed tissue, yielding the volatiles propyl 9.1 and 12.7 kg (20 and 28 lb) cartons, and as 24,
disulfide and methyl propyl disulfide. Major onion 36 and 48 count, bunched in 9.1, 5.0 and 5.9 kg
producers are China, India, United States, Turkey, (20, 11 and 13 lb) containers, respectively.
Japan, Spain, Holland, Poland, and Ukraine.
Grades of Bermuda-Granex-Grano type onions
include U.S. No. 1, U.S. No. 2, and U.S.
Quality Characteristics and Criteria Combination and are based on external appearance
and size. U.S. Combination consists of a mixture
High-quality onions have mature bulbs with of U.S. No. 1 and U.S. No. 2 onions in which at
good firmness and compactness of fleshy scales. least 50% (by weight) of the onions in each lot
Size, shape, and the color of the dry skin should meet the requirements of U.S. No. 1 grade. Bulb
be typical for the variety. They should be free of diameters are defined as follows:
mechanical or insect damage, decay, sunscald
injury, greening of fleshy scales, sprouting, Small, 2.5 to 5.7 cm (1 to 2.25 in); Prepacker,
bruising, doubles, bottlenecks (onions which 4.5 to 7.6 cm (1.75 to 3 in); Medium, 5.1 to 8.3
have abnormally thick necks with only fairly well cm (2 to 3.25 in.); Large or Jumbo, 7.6 to 9.5 cm
developed bulbs), and any other defects. (3 to 3.75 in); and Colossal, >9.5 cm (3.75 in).
Containers vary from 2.3 to 22.7 kg (5 to 50 lb)
with 9.1 kg (20 lb) being the most common size.
Horticultural Maturity Indices
For onions other than Bermuda-Granex-Grano and
Harvest maturity depends on the purpose for Creole types, there are five Grades:
which the onions are grown. Onions intended for
storage should be harvested when 50 to 80% of U.S. No. 1, U.S. Export No. 1, U.S. Commercial,
the tops have fallen over and bulbs are mature U.S. No. 1 Boilers, U.S. No. 1 Picklers, and U.S.
with a thin neck. Yields are higher if they are No. 2. The sizing of onions in this group is the
harvested after the tops are completely dry, but same as above. Containers are usually 9.1 kg (20
then bulbs tend to have a shorter storage life. To lb), except for export onions, which are packed in
hasten maturity, tops can be rolled with a light 25-kg (56-lb) containers.
roller when 10% of them have fallen. About 7
436
Precooling Conditions conditions during growth. Maleic hydrazide, a
sprouting inhibitor, is often used to prevent root
In order to maintain high quality, bunched growth and sprouting during long-term storage.
green onions should be precooled to <4 °C (39 It is applied 2 weeks before harvest, when bulbs
°F) within 4 to 6 h of harvest. Hydrocooling, are mature and 50% of tops are down, but onion
forced-air cooling, and vacuum-cooling are used plants must still have five to eight green leaves in
with crushed ice over the product to maintain order to absorb and translocate the sprout inhibitor
temperature and moisture. to bulbs.
Dry onion bulbs for long-term storage should Onions intended for storage should be dried well
be precooled to 0 °C (32 °F) immediately after and cured in the field, under sheds, or in storage.
drying, or within 1 mo using cool outside air. After 2 weeks of field drying, onions can be
The precooling method affects storability. Rapid transferred to storage rooms for final drying and
precooling inhibits rooting and sprouting during curing. Forced-air ventilation at 25 to 27 °C (77
storage. Natural cooling (slow) has a positive to 81 °F) using outside or heated air is commonly
effect on storability when onions have a long rest used to dry onions. Onions can be stored and
period and weather conditions are good for curing. dried on the floor in bulk 3 to 4 m deep or in 500
Gradual cooling at 1 °C (1.8 °F) per day in storage to 1,000 kg (1,100 to 2,200 lb) boxes. Drying
is less effective at inhibiting sprouting and rooting is complete when the onion neck is tight, outer
than rapid cooling (Grzegorzewska 1999). scales are dry and make a rustling noise when
touched, and the skin color is uniform. Weight loss
of 3 to 5% can occur during drying. Losses from
Optimum Storage Conditions neck rot are reduced by rapid drying immediately
after harvest. After drying and curing, the
Bunched green onions can be stored 3 to 4 temperature should be lowered gradually to the
weeks at 0 °C (32 °F) with 95 to 98% RH. normal seasonal temperature, or bulbs can be
Under these conditions, bunched onions stored precooled in cold storage at 0 °C (32 °F). In
in polyethylene-lined containers and top-iced either case, condensation should be avoided as it
maintain excellent quality for 1 mo. Storage life encourages rot and changes the color of the dry
decreases to 1 week if the temperature is 5 °C skin.
(41 °F), and rapid yellowing and decay of leaves
occurs at higher temperatures. In most European countries and in the Northern
United States, onions are stored in common
Pungent dry onions can be stored for 6 to 9 mo storage, using cool, ambient air to maintain
at 0 °C (32 °F) with 65 to 75% RH. High RH optimum temperature and RH. In this condition,
induces root growth, while high temperature onions are usually stored only until the end of
induces sprouting. A combination of high March or beginning of April, since further storage
temperature and high RH increases rotting and can cause losses to sprouting and rotting.
decreases quality. Storage below the freezing point
of -1 to -2 °C (28 to 30 °F) is recommended in Refrigerated storage is used for onions that are
Europe. Mild type or sweet onions can be kept for to be marketed in late April to early July. For
1 to 3 mo; they are stored in common storage with cold storage, onions are usually packed in crates
cool, circulating ambient air or in refrigerated cold or containers. Air circulation must be sufficient
rooms. Onions grown from seed store better than to maintain a constant temperature and remove
those grown from sets or transplants. moisture from inside storage containers. Onions
packed in sacks can only be stored for a limited
After harvest, onion bulbs enter a state of rest for period, about 1 mo, since air movement through
4 to 6 weeks, depending on cultivar and weather sacks is insufficient to maintain proper storage
437
conditions. When stored below -1 to -2 °C (28 to Retail Outlet Display Considerations
30 °F), onions should be thawed at 5 °C (41 °F)
for 1 to 2 weeks before they are removed from Onion bulbs can be displayed in small packages
storage. Rapid thawing damages onion bulbs. or in bulk at <5 °C (41 °F). This temperature
effectively retards sprouting for up to 7 mo.
Mild and sweet onions can be stored for only 1 Onions should not be stored with fruits and
to 4 mo, even in optimal cold storage. CA may vegetables that tend to absorb odors.
extend the storage period. Onions tolerate storage
at 30 to 35 °C (86 to 95 °F) for short periods
before marketing or processing, but their quality Chilling Sensitivity
and external color is less attractive than that of
cold-stored onions. Onions are not sensitive to chilling and can be
stored at -2 to -3 °C (28 to 27 °F), since the
highest freezing point is -0.8 °C (31.6 °F). Storage
Controlled Atmosphere (CA) at <-4 °C (25 °F) may cause freezing injury.
Considerations
Bunched green onions can be stored for 6 to 8 Ethylene Production and Sensitivity
weeks in 2% O2 and 5% CO2 at 0 °C (32 °F). They
can tolerate 1% O2 and up to 5% CO2, but off Ethylene production is very low: < 0.1 µL
flavor may develop if they are stored at >5 °C (41 kg-1 h-1 at 20 °C (68 °F) (Suslow and Cantwell
°F) (Suslow and Cantwell 1998). 1998). Sensitivity to ethylene is also low, but
concentrations of >1,500 µL L-1 encourage
Low-O2 atmospheres reduce respiration and sprouting of onion bulbs.
extend storage life, while elevated CO2 reduces
sprouting and root growth. CA has been used
for storage of pungent onions in England, Respiration Rates
Switzerland, and Poland (Adamicki 1998).
An atmosphere of 3% O2 and 5% CO2 inhibits Temperature mg CO2 kg-1 h-1
rooting, sprouting ,and disease development 0 °C 3
(Adamicki and Kepka 1974, Smittle 1988). 5 °C 5
However, onions stored in 3% O2 and 10% CO2 10 °C 7
showed physiological disorders; high CO2 caused 15 °C 7
injury, but neck rot (Botrytis spp.) was reduced 20 °C 8
(Sitton et al. 1997). Data from Robinson et al. (1975).
CA can have also residual effects. After storage To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
for up to 226 days in 3% O2 and 5% CO2, by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
sprouting of ‘Wolska’ onions at 20 °C (68 °F) 1.8 at 20 °C (68 °F). To calculate heat production,
was delayed for 10 days, compared to air-stored multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
controls (Adamicki and Kepka 1974). It is or by 61 to get kcal tonne-1 day-1.
possible to store onions in low O2 (1 to 2%) with
2% CO2 (Adamicki 1998, Tanaka et al. 1996).
There is some commercial use of 3% O2 and 5 to Physiological Disorders
7% CO2 for sweet onion cultivars (Smittle 1989,
Mikitzel et al. 1993, Sitton et al. 1997). Onion bulbs are affected by several physiological
disorders. Freezing injury causes soft, water-
soaked, fleshy scales and rapid decay after
438
transfer from cold storage to higher temperature, Blue mold rot also produces watery soft rot of
which results in microbial growth. Translucent neck and outer scales, followed by formation of
scales resemble freezing injury and are prevented blue to blue-green mold of the fungus Penicillium
by prompt cold storage following curing. spp. Harvest of mature bulbs, proper curing,
Translucent scales occur with loss of or changes and storage at 0 °C (32 °F) with 60 to 70% RH
in carbohydrate content. Storage of onions at >7% minimizes blue mold problems.
CO2 can also lead to development of translucent
scales. Late harvesting and a long drying period at Bacterial soft rot, caused by Erwinia carotovora
high temperatures produce the highest incidence Jones, develops water-soaked individual scales,
of translucent scales. or the entire onion, with foul smelling, viscous,
liquid-covered rotted areas. The disease progresses
Watery scales is a thick leathery skin with watery, rapidly under warm, humid conditions. Harvesting
glassy, fleshy scales below. The watery scales at full maturity, proper drying, minimizing
may later be affected by fungal or bacterial bruising, and maintaining optimum storage
growth. Late harvesting and prolonged field conditions prevent bacterial soft rot (Ryall and
drying produce the highest occurrence of leathery Lipton 1983, Suslow and Cantwell 1998).
skin. Scale greening, a green coloration of outer
scales, is caused by exposure to light after curing.
Ammonia injury is indicated by brown-black Quarantine Issues
blotches resulting from leakage of ammonia
during storage (Adamicki and Kepta 1974, Smittle Onions intended for export should be free from
1988, Hoftun 1993, Suslow and Cantwell 1998, Ditylenchus dipsaci Kühn.
Solberg 1999).
439
Hoftun, H. 1993. Internal atmosphere and watery Tanaka, K., Y. Matsuo, and J. Egashira. 1996.
scales in onion bulbs (Allium cepa L.). Acta Hort. Controlled atmosphere storage for onions. In
343:135-140. T. Kozai, ed., Proceedings of the International
Symposium on Plant Products in Closed
Mikitzel, L.J., M.E. Patterson, and H. Waelti. Ecosystems, Narita, Japan, 1996, pp. 669-674.
1993. The effect of CA storage on the postharvest Acta Hort., Leuven, Belgium.
life of sweet onions. In 6th International
Controlled Atmosphere Research Conference,
Ithaca, NY, June 1993, NRAES-71, vol. 2,
pp. 789-796. Northeast Regional Agricultural
Engineering Service, Cornell University, Ithaca,
NY.
440
Orange Horticultural Maturity Indices
442
Retail Outlet Display Considerations (Brown 1992) and stem-end rot (Barmore and
Brown 1985), especially at >10 μL kg-1 h-1.
Oranges should be displayed on nonrefrigerated Ethylene also increases respiration in citrus.
shelves and inspected often to remove damaged or
decaying fruit. Conditions for degreening (Kader and Arpaia
1992, Wardowski 1996) are—
Florida California
Chilling Sensitivity
Temperature 28 to 29 ºC 20 to 25 ºC
-1
California and Arizona oranges may develop Ethylene 5 µL L 5 to 10 µL L-1
chilling injury when held at temperatures below RH 90 to 96% 90%
about 3 to 5 ºC (37 to 41 ºF). Oranges produced Ventilation
in Florida or Texas rarely show chilling injury. (<0.1% CO2) 1 air change 1 to 2 air changes
per hour per hour
Symptoms of chilling injury include pitting,
Air Circulation 100 ft3 min-1 1 room volume
brown staining, increased decay, internal per 900-lb bin per min
discoloration, off flavors, and watery breakdown
that may take 60 days to develop at 5 °C (41 °F)
or become evident 1 to 2 days after moving to
Respiration Rates
room temperature (about 72 °F). After removing
fruit from chilling temperatures, respiration and
Temperature mg CO2 kg-1 h-1
ethylene production both increase.
0 °C 2 to 6
The development and severity of chilling injury 5 °C 4 to 8
in citrus is influenced by both preharvest and 10 °C 6 to 10
postharvest factors. Preharvest factors include 15 °C 11 to 22
cultivar, weather conditions, and even location 20 °C 20 to 31
of fruit on the tree (sun-exposed fruit are more
susceptible to chilling injury). Postharvest To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
development of chilling injury symptoms can by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
be reduced by temperature conditioning before 1.8 at 20 °C (68 °F). To calculate heat production,
storage, use of high-CO2 atmospheres (for multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
example, in CA or through the use of wax coatings or by 61 to get kcal tonne-1 day-1.
or plastic film wraps), intermittent warming,
and use of benzimidazole fungicides (such as
thiabendazole and benomyl). The best means Physiological Disorders
of preventing chilling injury is storing fruit at
nonchilling temperatures. Creasing (albedo breakdown) results from the
irregular deterioration of albedo cells (white
spongy tissue) and the collapse of the overlaying
Ethylene Production and Sensitivity flavedo (colored portion of the rind) into irregular
grooves over the fruit surface. Such areas are
Citrus produce very little ethylene: <0.1 μL kg-1 weaker and often split, providing entry for
h-1 at 20 °C (68 °F). Ethylene is used to degreen pathogenic fungi and subsequent decay. This
oranges, especially early in the season when disorder is usually more common on thin-skinned,
natural degreening has been delayed because of fully mature fruit. Conditions giving rise to
warm night temperatures. Degreening usually creasing are complex and not well understood,
takes 1 to 3 days to complete and does not but appear to be related to cultivar, potassium
affect internal quality (SSC, TA, etc.). However, nutrition deficiencies, high levels of nitrogen,
ethylene stimulates decay, such as anthracnose rootstock, water status, and temperature during
443
fruit expansion. Because the disorder is associated Stem-end rind breakdown (SERB) is characterized
with advanced fruit maturity, earlier fruit by the irregular collapse and darkening of rind
harvesting may also reduce the problem. tissue around the stem end of the fruit. A narrow
ring of unaffected tissue immediately around
Granulation is caused by gel formation within the stem (button) is a distinctive symptom of
juice vesicles that greatly reduces extractable SERB. In some growing regions, SERB has been
juice content. It may occur primarily at the stem correlated with a preharvest imbalance in nitrogen
end (in ‘Valencia’ oranges), or extend through the and potassium. Postharvest, SERB is primarily
center of the fruit (in navel oranges). In the United associated with drying conditions and fruit water
States, this is considered a preharvest disorder loss, particularly between harvest and waxing.
that appears more in fruit exposed to the sun, fruit Postharvest practices that minimize water loss,
from young or water-stressed trees, overmature such as maintaining high RH during degreening,
fruit, or fruit from vigorously growing trees. In rapid handling, avoiding excessive brushing,
other parts of the world, the disorder also develops and promptly applying an even coat of wax, are
after harvest. currently the best means of reducing SERB.
444
Quarantine Issues References
Oranges are a fruit fly host, and when produced AMS [USDA Agricultural Marketing Service].
in areas where any fruit fly is found, must be 1969. United States Standards for Grades of
treated for insect control before shipment to Oranges (Texas and States Other Than Florida,
some markets. Approved disinfestation for California and Arizona). U.S. Department of
oranges include methyl bromide fumigation, Agriculture, Washington, DC.
cold treatments, and vapor heat treatments. Use
of methyl bromide is being phased out and will AMS [USDA Agricultural Marketing Service].
no longer be available by the year 2005. Cold 1997. United States Standards for Grades of
treatments are commonly used but may result Florida Oranges and Tangelos. U.S. Department of
in chilling injury. Irradiation and controlled Agriculture, Washington, DC.
atmospheres are potential alternative disinfestation
treatments. AMS [USDA Agricultural Marketing Service].
1999. United States Standards for Grades
All disinfestation treatments can result in of Oranges (California and Arizona). U.S.
phytotoxic injury to the fruit, with the degree of Department of Agriculture, Washington, DC.
injury depending on preharvest factors such as
cultivar and stage of maturity. As an alternative to Barmore, C.R., and G.E. Brown. 1985. Influence
disinfestation treatments, some production areas of ethylene on increased susceptibility of oranges
have established protocols that are accepted by to Diplodia natalensis. Plant Dis. 69:228-230.
receiving markets for certifying “fly-free” areas.
Oranges grown in these areas do not have to be Brown, G.E. 1992. Factors affecting the
treated before shipment. occurrence of anthracnose on Florida citrus. Proc.
Intl. Soc. Citricult. 3:1044-1048.
445
Kader, A.A., and M.L. Arpaia. 1992. Postharvest
handling systems: subtropical fruits. In
A.A. Kader, ed., Postharvest Technology of
Horticultural Crops, 2nd ed., pp. 233-240. DANR
Pub. no. 3311, University of California, Davis,
CA.
446
Papaya (Akamine and Goo 1971). Less mature fruit are
lower in sugar and ripen poorly.
Lili Zhou, Robert E. Paull, and Nancy Jung Chen
Zhou, Paull, and Chen are with the Department Grades, Sizes, and Packaging
of Tropical Plant and Soil Sciences, University of
Hawaii at Manoa, Honolulu, HI. The most common package size is a 4.5-kg
(10-lb) carton; larger 10-kg (22-lb) cartons are
also used. Cartons from areas requiring insect
Scientific Name and Introduction disinfestation are fully sealed to meet regulatory
requirements, while fruit from other areas can be
Papaya, papaw, or paw paw (Carica papaya L.) in open-topped cartons. Count size ranges from
is cultivated throughout the tropics for its fruit 6 to 18, depending on fruit and carton size. Fruit
(Nakasone 1986). Fruit are eaten green or ripe, in are marketed as color break, quarter, half, or
salads or fresh. The related Ecuadorian babaco (C. three-quarters ripe and are normally ready to eat
pentagonia) is also eaten fresh. when there is 75% or more skin color. Foam mesh
sleeves, foam padding on the bottom of cartons, or
Fruit are pyriform (pear-shaped), spherical, or paper wrapping prevents abrasion injury, a major
cylindrical. The pyriform, hermaphroditic fruit problem in fruit still having green areas of skin
is the most common in commerce. These belong (Quintana and Paull 1993).
to the ‘Solo’ group and includes the cultivars
‘Kapoho,’ ‘Rainbow,’ ‘Sunup,’ ‘Sunrise,’ and
‘Sunset’; these varieties weigh 300 to 700 g (10 Precooling Conditions
to 25 oz). Other varieties range from 200 g to 10
kg (0.4 to 22 lbs), with flesh 1.5 to 4 mm (0.06 to Room-cooling and forced-air cooling are
0.16 in) thick. Flesh is greenish-white in immature commonly used. Hydrocooling is possible.
fruit to pale orange-yellow, salmon pink, or red, However, rapid cooling after insect disinfestation
depending on cultivar, when ripe. treatments can lead to skin scalding.
Size, shape, a smooth skin, and absence of Store at 7 to 13 °C (45 to 55 °F) with 90 to 95%
blemishes are major quality characteristics. RH. At 7 to 10 °C (45 to 50 °F), storage life is
Consumers in Western countries also prefer limited by chilling injury, while at 10 to 13 °C
fruit without a heavy musky, sweaty odor found (50 to 55 °F) fruit ripens slowly (Chen and Paull
in some Southeast Asian cultivars. Small, 1986). Papaya fruit at color-turning (break) stage
dry, brown-black “freckles” on the skin are can be stored at 7 °C (45 °F) for 14 days and
nonpathogenic and do not detract from ripening or will ripen normally when transferred to room
flavor (Reyes and Paull 1994). temperature (Thompson and Lee 1971, Chen and
Paull 1986). Ripe, full-color fruit can be held for
>1 week at 1 to 3 °C (34 to 37 °F).
Horticultural Maturity Indices
447
O2 for 6 days (Akamine and Goo 1969). Low O2 Chilling Sensitivity
(1 to 5%), with or without high CO2 (2 to 10%),
reduces decay (Hatton and Reeder 1969) and Chilling injury symptoms include skin scald, hard
delays ripening (Akamine 1959, Chen and Paull lumps in the pulp around vascular bundles, and
1986). High CO2 (30%) adversely affects internal water-soaking of flesh (Thompson and Lee 1971,
color, aroma, and flavor, while there is no residual El-Tomi et al. 1974, Chen and Paull 1986). Fruit
effect of 10% CO2 on decay control, though skin become progressively less susceptible to chilling
degreening is delayed. stress as they ripen (Chen and Paull 1986).
Symptoms of chilling injury occur after 14 days
At 10 °C (50 °F), fruit can be stored for 36 days at 5 ºC (41ºF) in mature green fruit and 21 days
in 8% CO2 and 3% O2 and still have 5 days in 60% yellow fruit. Skin scald can be induced in
at 25 °C (77 °F) for retail (Cenci et al. 1997). color-break fruit after chilling at 1 ºC (34 ºF) for
Ethylene removal prior to storage has shown 24 h. At a storage temperature of 7 ºC (45 ºF) for
variable results (Nazeeb and Broughton 1978). 14 days, storage decay is less than when fruit are
CA recommendations are 2 to 5% O2 plus 5 to held at 12 to 13 ºC (54 to 55 ºF) (Arisumi 1956,
8% CO2. However, no commercial use has so El-Tomi et al. 1974).
far been reported (Yahia 1998). Fruit stored at
10 °C (50 °F), 98% RH, and low pressure of 20
mm Hg ripened more slowly than fruit at normal Ethylene Production and Sensitivity
atmospheric pressure. Low-pressure storage
appears to suppress disease development (Alvarez Ethylene rates in ripening fruit are 6 to 10 μL kg-1
1980, Chau and Alvarez 1983). h-1 (Paull and Chen 1983, Paull 1993). Ethylene-
treated papaya ripen faster and more uniformly
in terms of skin degreening, softening, and flesh
Retail Outlet Display Considerations color (An and Paull 1990). Since papaya ripen
from the inside outwards, the effect of ethylene
The optimum temperature for fruit ripening is 23 treatment is to accelerate the rate of ripening of
to 28 °C (73 to 82 °F), with fruit taking 10 to 16 the mesocarp tissue nearer the skin that has not
days to reach full skin yellowing from the color- started to soften. Ethylene is not recommended
break stage (An and Paull 1990). Severe weight commercially, as rapid softening severely limits
loss and external abnormalities become significant available marketing time (An and Paull 1990).
at temperatures higher than 28 °C (82 °F). Display
temperatures should not be less than 10 °C (50
°F) if fruit are not fully ripe. Fully ripe fruit at the Respiration Rates
edible stage can be held at 1 to 3 °C (34 to 37 °F).
Fruit should not be stacked more than two or three Papaya are climacteric and begin to yellow from
deep in racks, and wicker baskets with uneven the blossom end (Akamine 1966).
bottoms and sides should be avoided; or at least a
layer of protection should be placed between racks Temperature mg CO2 kg-1 h-1
and fruit (Paull et al. 1997). Loss of about 8% of 5 °C 4 to 6
weight from color break produces rubbery, low- 15 °C 15 to 22
gloss, unmarketable fruit (Paull and Chen 1989). 20 °C - color break 9 to 18
Diseased and bruised fruit should be removed 20 °C - ripe 70 to 90
from display and used immediately, if possible, in
salads or mixed fruit cocktails. Papaya should not To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
be misted. by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
448
Physiological Disorders [Geijskes]) generally causes this damage in
Hawaii.
A number of nonpathological disorders are seen
in marketed fruit (Paull et al. 1997). Green,
slightly sunken areas on ripe yellow fruit are Postharvest Pathology
caused by abrasion injury when fruit were still
green (Quintana and Paull 1993). Unsightly skin The major postharvest diseases are anthracnose
freckles (small, brown, slightly raised areas) and stem end rot. Postharvest diseases, especially
that are more common on the side of the fruit anthracnose, become a problem when fruit have
exposed to the sun is seasonal, developing when 25% or more skin yellowing (Wardlaw et al.
rainfall and low temperatures occur 2 mo before 1939, Alvarez and Nishijima 1987). Papaya
harvest (Reyes and Paull 1994). This disorder is diseases greatly increase in severity and incidence
nonpathogenic and does not influence ripening or following 4 weeks storage at 10 °C (50 °F).
flavor. Sunscald, a dark-olive-brown discoloration, Mechanical injury and chilling injury can enhance
occurs on fruit developing on trees with very development of postharvest diseases (Somner
sparse foliage, on trees that are leaning over and Mitchell 1978, Alvarez and Nishijima 1987,
with fruit directly exposed to the sun, and where Nishijima et al. 1990). Rhizopus requires breaks
harvested fruit are left exposed to the sun. in the cuticle for the disease to develop (Nishijima
et al. 1990). Cuticle disruption occurs as latex
Blossom end defect can be severe at some times vessels break down, when the fruit is 40 to 60%
of the year (Zee et al. 1989). It leaves an open yellow (Paull and Chen 1989). Fruit fly punctures
channel at the blossom end to the fruit seed cavity. can also increase Rhizopus rot (Hunter and
Fruit with this disorder are prone to bacterial Buddenhagen 1972), as can mechanical injuries
diseases in the fruit seed cavity before harvest. and lesions caused by fungi such as anthracnose
and Cercospora black spot (Nishijima et al. 1990).
Hard lumps in the flesh occur infrequently in Postharvest diseases are effectively controlled by
otherwise ripe, non-heat-treated fruit (Magalona hot water at 49 °C (120 °F) for 20 min (Akamine
1963, Cavaletto 1989). The lumps are thought to and Arisumi 1953, Couey et al. 1984, Glazener
be associated with insect injury, disease, or other et al. 1984) and fungicide treatment (Couey and
foreign material in the flesh. The condition can Farias 1979).
also occur in heat-treated fruit. Premature ripening
of fruit, referred to as “soft fruit,” is related to low
calcium content. This condition is more common Quarantine Issues
following periods of heavy rainfall 2 to 3 mo
before harvest, at the start of the final phase of Fruit fly infestation becomes a problem with
fruit growth (Qiu et al. 1995). papaya after fruit have 25% skin yellowing (Seo et
al. 1982). The damage caused by fruit flies include
Intra-ovarian ovaries are common in some strains small surface blemishes, destruction of the edible
of papaya (Nakasone and Arkle 1971). These flesh, and spoilage from decay. Heat treatments
ovaries occur as a proliferation of tissue in the and irradiation are used for
seed cavity and can be a threadlike appendage to fruit fly disinfestation (Couey 1989, Paull 1990,
round or elongated structures of various sizes and Armstrong 1994). Occasionally, heat treatment
shapes. A few fill the entire seed cavity of the fruit can cause internal injury and scald (Paull
and have their own seed cavity. 1995), limit postharvest life, and reduce quality
(Paull 1990). Papaya can tolerate insecticidal
Sunken, dry, brownish-grey areas are caused by atmospheres (0.17 to 0.35% O2, balance N2) at 20
mites feeding on skin during early fruit growth. °C (68 °F) for up to 5 days (Yahia et al. 1989),
The red and black mite (Brevipalpus phoenicis though its disinfestation potential has not been
shown.
449
Suitability as Fresh-Cut Product An, J.F., and R.E. Paull. 1990. Storage
temperature and ethylene influence on ripening of
Fruit are prepared as deseeded, halved fruit, slices, papaya fruit. J. Amer. Soc. Hort. Sci. 115:949-953.
and chunks. Fresh-cut products made from 60
to 80% yellow-skinned fruit, overwrapped with Arisumi, T. 1956. Test shipments of papaya with
plastic film, can be held for up to 3 weeks at 0 to special reference to storage decay control. Tech.
4 °C (32 to 39 °F) (Paull and Chen 1997). Powrie Bull. 29, Hawaii Agricultural Experiment Station,
et al. (1990) patented a procedure in which pieces Honolulu, HI.
dipped in citric acid held in multilayer bags with
15 to 20% O2 and 3% helium had little loss in taste Armstrong, J.W. 1994. Tropical and subtropical
and texture after 16 weeks at 1 ºC (34 °F). fruits. In R.E. Paull and J.W. Armstrong, eds.,
Insect Pests and Fresh Horticultural Products:
Treatments and Responses, pp. 275-290. CAB
References Intl., Wallingford, U.K.
Akamine, E.K. 1959. Effects of carbon dioxide Cavaletto, C.G. 1989. Quality of high temperature
on quality and shelf life of papaya. Tech. Progr. forced air treated papaya. In Proceedings of
Rpt. 120, Hawaii Agricultural Experiment Station, the 25th Annual Papaya Industry Association
Honolulu, HI. Conference, Sept. 1989, Hilo, HI, pp. 10-15.
Akamine, E.K. 1966. Respiration of fruits Cenci, S.A., A.G. Soares, J.M.S. Bibino, and
of papaya (Carica papaya L. var. Solo) with M.L.M. Soiya. 1997. Study of the storage of
reference to effect of quarantine disinfestation ‘Sunrise’ and ‘Solo’ papaya fruits under control
treatments. Proc. Amer. Soc. Hort. Sci. 89:231- atmospheres. Abstract no. 112. In M.E. Saltveit,
236. ed., 7th International Controlled Atmosphere
Research Conference, July 13-18, 1997,
Akamine, E.K., and T. Arisumi. 1953. Control University of California, Davis, vol. 4, University
of postharvest storage decay of papaya (Carica of California, Davis, CA.
papaya L.) with special reference to the effect of
hot water. Proc. Amer. Soc. Hort. Sci. 61:270-274. Chau, K.F., and A.M. Alvarez. 1983. Effects
of low-pressure storage on Colletotrichum
Akamine, E.K., and T. Goo. 1969. Effects of gloeosporioides and postharvest infection of
controlled atmosphere storage of fresh papaya papaya. HortScience 18:953-955.
(Carica papaya, L. var. Solo) with special
reference to shelf life extension of fumigated fruit. Chen, N.M., and R.E. Paull. 1986. Development
Res. Bull. 144, Hawaii Agricultural Experiment and prevention of chilling injury in papaya fruit. J.
Station, Honolulu, HI. Amer. Soc. Hort. Sci. 111:639-643.
Akamine, E.K., and T. Goo. 1971. Relationship Couey, H.M. 1989. Heat treatment for control
between surface color development and total of postharvest diseases and insect pests of fruits.
soluble solids in papaya. HortScience 6:567-568. HortScience 24:198-202.
Alvarez, A.M. 1980. Improved marketability of Couey, H.M., A.M. Alvarez, and M.G. Nelson.
fresh papaya by shipment in hypobaric containers. 1984. Comparison of hot-water spray and
HortScience 15:517-518. immersion treatments for control of postharvest
decay of papaya. Plant Dis. 68:436-437.
Alvarez, A.M., and W.T. Nishijima. 1987.
Postharvest diseases of papaya. Plant Dis. 71:681-
686.
450
Couey, H.M., and G. Farias. 1979. Control of Paull, R.E. 1990. Postharvest heat treatments and
postharvest decay of papaya. HortScience 14:719- fruit ripening. Postharv. News Info. 1:355-363.
721.
Paull, R.E. 1995. Preharvest factors and the heat
El-Tomi, A.L., A.B. Abon-Aziz, A.S. Abdel- sensitivity of field grown ripening papaya fruit.
Kader, and F.K. Abdel-Wahab. 1974. The effect of Postharv. Biol. Technol. 6:167-175.
chilling and non-chilling temperatures on quality
of papaya fruit. Egypt. J. Hort. 1:179-185. Paull, R.E. 1993. Pineapple and papaya. In
G. Seymour, J. Taylor, and G. Tucker, eds.,
Glazener, J.A., H.M. Couey, and A. Alarez. 1984. Biochemistry of Fruit Ripening, pp. 291-323.
Effect of postharvest treatments on stemphyllium Chapman & Hall, London, U.K.
rot of papaya. Plant Dis. 68:986-988.
Paull, R.E., and N.J. Chen. 1983. Postharvest
Hatton, T.T., and W.F. Reeder. 1969. Controlled variation in wall degrading enzymes of papaya
atmosphere storage of papaya. Proc. Trop. Reg. fruit during ripening. Plant Physiology 72:131-
Amer. Soc. Hort. Sci. 13:251-256. 138.
Hawaii Department of Agriculture. 1990. Paull, R.E., and N.J. Chen. 1989. Waxing and
Standards for Hawaii-Grown Papaya. Hawaii plastic wraps influence water loss from papaya
Department of Agricuture, Marketing Division, fruit during storage and ripening. J. Amer. Soc.
Honolulu, HI. Hort. Sci. 114:937-942.
Hunter, J.E., and I.W. Buddenhagen. 1972. Paull, R.E., and N.J. Chen. 1997. Minimal
Incidences, epidemiology and control of fruit processing of papaya (Carica papaya L.) and
disease of papaya in Hawaii. Trop. Ag. 49:61-71. the physiology of halved fruit. Postharv. Biol.
Technol. 12:93-99.
Magalona, N. 1963. Artificial Induction of Lumps
in the Fruit of Papaya (Carica papaya L.) M.Sc. Paull, R.E., W. Nishijima, M. Reyes, and C.
Thesis, University of Hawaii, Honolulu, HI. Cavaletto. 1997. A review of postharvest handling
and losses during marketing of papaya (Carica
Nakasone, H.Y. 1986. Papaya. In S.P. papaya L). Postharv. Biol. Tech. 11:165-179.
Monselise, ed., CRC Handbook of Fruit Set and
Development, pp. 277-301. CRC Press, Boca Powrie, W.D., R. Chiu, H. Wu, and B.J. Skura.
Raton, FL. 1990. Preservation of cut and segmented fresh
fruit pieces. U.S. Patent No. 4,895,729.
Nakasone, H.Y., and T.D. Arkle. 1971.
Development of intra-ovarian ovaries in Carica Qiu, Y., M.S. Nishina, and R.E. Paull. 1995.
papaya L. J. Amer. Soc. Hort. Sci. 96:550-553. Papaya fruit growth, calcium uptake and fruit
ripening. J. Amer. Soc. Hort. Sci. 120:246-253.
Nazeeb, M., and W.J. Broughton. 1978. Storage
conditions and ripening of papaya ‘Bentong’ and Quintana, M.E.G., and R.E. Paull. 1993.
‘Taiping.’ Sci. Hort. 9:265-277. Mechanical injury during postharvest handling
of ‘Solo’ papaya fruit. J. Amer. Soc. Hort. Sci.
Nishijima, W.T., S. Ebersole, and J.A. Fernandez. 118:618-622.
1990. Factors influencing development of
postharvest incidence of Rhizopus soft rot of Reyes, M.E.Q., and R.E. Paull. 1994. Skin
papaya. Acta Hort. 269:495-502. freckles on ‘Solo’ papaya fruit. Sci. Hort. 58:31-
39.
451
Seo, S.T., G.B. Farias, and E.J. Harris. 1982.
Oriental fruit fly: ripening of fruit and its effect on
index of infestation of Hawaiian papayas. J. Econ.
Ent. 75:173-178.
452
Parsley Grades, Sizes, and Packaging
Julian A. Heyes Only one grade is available (U.S. No. 1), which
consists of parsley that meet quality criteria and
Heyes is formerly with the New Zealand Institute have similar varietal characteristics; that is, curly-
for Crop and Food Research, Palmerston North, leaf and flat-leaf varieties are not mixed. Parsley is
New Zealand. He is now with the Department of usually packaged in cartons or jumbo crates of 60
Food Science and Technology, Massey University, bunches, 9 to 11 kg (20 to 25 lb).
Palmerston North, New Zealand.
Precooling Conditions
Scientific Name and Introduction
Rapid removal of field heat without excessive
Petroselinum crispum (Mill.) Nyman ex A.W. drying helps retain green color and freshness.
Hill—parsley—is a member of the Apiaceae Parsley can be precooled with ice (package icing
family. The edible foliage is grown as an annual or liquid-icing [Cantwell and Reid 1992]) or by
and used as a garnish and food ingredient. Both vacuum-cooling (Aharoni et al. 1989). Forced-air
curly-leaved (such as ‘Deep Green,’ ‘Forest cooling or hydrocooling are commonly practiced
Green,’ and ‘Moss Curled’) cultivars and flat- (Joyce et al. 1986).
leaved (such as Plain, Plain Italian Dark Green,
and Deep Green Italian) types are available.
There is a subspecies, P. crispum subsp. Optimum Storage Conditions
tuberosum, Hamburg parsley, that has an edible
root. Parsley has very high vitamin and nutrient The recommended conditions for commercial
content. It is highest in calcium, iron, and folate storage of parsley are 0 °C (32 °F) with 95 to
of all vegetables studied (Athar et al. 1999) and 100% RH (Cantwell 2002). Parsley can be stored
has among the highest contents of β-carotene, for 1 to 2 mo under these conditions, compared to
thiamin, riboflavin, and vitamins C and E. A high only 3 days at 18 to 20 °C (64 to 68 °F) with 85 to
proportion of the carotene is 9-cis β-carotene, 90% RH (Lisiewska et al. 1997). The endpoint of
possibly active against cancer and cardiovascular storage at 0 °C (32 °F) occurs when parsley wilts,
disease (Benamotz and Fishler 1998). at around 20% weight loss (Hruschka and Wang
1979). MAP is effective in extending storage
life, but temperature changes and condensation
Quality Characteristics and Criteria must be avoided. Aharoni et al. (1989) showed
that nonperforated polyethylene liners delayed
The quality criteria for parsley are freshness, yellowing and decay at low temperature. Park
green color, freedom from defects or seed stems, et al. (1999) achieved 77 days of storage at 0 °C
and freedom from decay (AMS 2002). (32 °F) and 35 days at 5 °C (41 °F) with good
retention of firmness and vitamin C content by
using a 40 µm-thick ceramic film. A preharvest
Horticultural Maturity Indices spray with gibberellic acid may extend storage life
(Lers et al. 1998). Hamburg parsley roots (without
Parsley can be harvested progressively or cut leaves) can be stored at 0 °C (32 °F) for several
all at one time. Long petioles are desirable for months (Bakowski et al. 1994, Elkner et al. 1998).
bunching. Most of the U.S. crop is harvested by
hand.
453
Controlled Atmosphere (CA) Respiration Rates
Considerations
Temperature mg CO2 kg-1 h-1
Parsley can tolerate 8 to 10% O2 and 8 to 10% 0 °C 22 to 38
CO2 (Saltveit 1997), but this may be of little 5 °C 49 to 70
benefit at 0 °C (32 °F). An atmosphere of 10% O2 10 °C 78 to 150
and 11% CO2 was optimal for delaying yellowing 15 °C 131 to 168
in parsley stored at 5 °C (41 °F) (Apeland 1971). 20 °C 176 to 221
Storage in 10% O2 and 10% CO2 (Yamauchi and 25 °C 259 to 289
Watada 1993) or 10% CO2 (Lers et al. 1998) Data from Apeland (1971), Hruschka and Wang
delayed yellowing at room temperature. (1979), and Cantwell and Reid (1986).
454
staff is paramount. Parsley has been implicated Benamotz, A., and R. Fishler. 1998. Analysis of
as a source of the infectious Shigella (Crowe carotenoids with emphasis on 9-cis β-carotene
et al. 1999) and Citrobacter freundii, causing in vegetables and fruits commonly consumed in
gastroenteritis and hemolytic uremic syndrome Israel. Food Chem. 62:515-520.
(Tschape et al. 1995), and thermotolerant
campylobacters (Park and Sanders 1992). Cantwell, M. 2002. Properties and recommended
conditions for storage of fresh fruits and
vegetables. At http://postharvest.ucdavis.edu/
Special Considerations produce_information/storagefreshproduce.
Parsley has an extremely high respiration rate. Cantwell, M.I., and M.S. Reid. 1986. Postharvest
Young leaves respire at a higher rate than old handling of fresh culinary herbs. II. Respiration
leaves at harvest, but the respiration rate does not and ethylene production. Perishables Handling
decrease as much after harvest in older leaves as Quarterly 60:2-5.
in younger leaves, so younger leaves store better
(Apeland 1971). Cantwell, M.I., and M.S. Reid. 1992. Postharvest
handling systems: fresh herbs. In A.A. Kader, ed.,
Parsley contains furocoumarins, including Postharvest Technology of Horticultural Crops,
psoralen (Manderfeld et al. 1997), which are 2nd ed., pp. 211-213. DANR Pub. no. 3311,
effective antimicrobial agents but can act as University of California, Oakland, CA.
phototoxins, inducing dermatitis (Lagey et al.
1995). Parsley is used as a medicinal plant to treat Cantwell, M.I., and M.S. Reid. 1993. Postharvest
hypertension in Morocco (Ziyyat et al. 1997) and physiology and handling of fresh culinary herbs. J.
diabetes in Turkey (Tunali et al. 1999). Herbs, Spices and Medicinal Plants 1:93-127.
Athar, N., T.W. Spriggs, and P. Liu. 1999. The Hruschka, H.W., and C.Y. Wang. 1979. Storage
Concise New Zealand Food Composition Tables, and shelf life of packaged watercress, parsley
4th ed. New Zealand Institute for Crop & Food and mint. Marketing Research Report 1102, U.S.
Research, Palmerston North, N.Z. Department of Agriculture, Washington, DC.
Bakowski, J., H. Michalik, and L. Umiecka. Inaba, A., Y. Kubo, and R. Nakamura. 1989. Effect
1994. Effect of sowing and harvest date on the of exogenous ethylene on respiration in fruits and
quality of root parsley and its storability. Biuletyn vegetables with special reference to temperature.
Warzywniczy 41:157-167. J. Jap. Soc. Hort. Sci. 58:713-718.
455
Joyce, D., M.S. Reid, and P. Katz. 1986. Tschape, H., R. Prager, W. Streckel, et al. 1995.
Postharvest handling of fresh culinary herbs. Verotoxinogenic Citrobacter freundii associated
Perishables Handling Quarterly 58:1-4. with severe gastroenteritis and cases of hemolytic
uraemic syndrome in a nursery school—green
Lagey, K., L. Duinslaeger, and A. Vanderkelen. butter as the infection source. Epidem. Inf.
1995. Burns induced by plants. Burns 21:542-543. 114:441-450.
Lers, A., W.B. Jiang, E. Lomanies, and N. Tsumura, F., Y. Ohsako, Y. Haraguchi. 1993.
Aharoni. 1998. Gibberellic acid and CO2 additive Rapid changes in parsley leaves during storage in
effect in retarding postharvest senescence of controlled or ethylene-containing atmosphere. J.
parsley. J. Food Sci. 63:66-68. Food Sci. 58:619-625.
Lisiewska, Z., W. Kmiecik, and A. Budnik. Tunali, T., A. Yarat, R. Yanardag, et al. 1999.
1997. Effect of conditions and time of storage on Effect of parsley (Petroselinum crispum) on the
technological quality changes of parsley leaves. skin of STZ-induced diabetic rats. Phytotherapy
Folia Hort. 9:21-29. Res. 13:138-141.
Manderfeld, M.M., H.W. Schafer, P.M. Davidson, AMS [USDA Agricultural Marketing Service].
and E.A. Zottola. 1997. Isolation and identification 2002. Quality Standards—Fresh Fruits and
of antimicrobial furocoumarins from parsley. J. Vegetables. At http://www.ams.usda.gov/
Food Protect. 60:72-77. AMSv1.0/standards.
Manzano, M., B. Citterio, M. Maifreni, et al. Yamauchi, N., and A.E. Watada. 1993. Pigment
1995. Microbial and sensory quality of vegetables changes in parsley leaves during storage in
for soup packaged in different atmospheres. J. Sci. controlled or ethylene-containing atmosphere. J.
Food Agric. 67:521-529. Food Sci. 58:616-618, 637.
Park, C.E., and G.W. Sanders. 1992. Occurrence Ziyyat, A., A. Legssyer, H. Mekhfi, et al. 1997.
of thermotolerant campylobacters in fresh Phytotherapy of hypertension and diabetes in
vegetables sold at farmers outdoor markets and oriental Morocco. J. Ethnopharmacol. 58:45-54.
supermarkets. Can. J. Microbiol. 38:313-316.
456
Parsnip Precooling Conditions
Quality Characteristics and Criteria Little work has been done, but limited results
suggest that there are no benefits to controlled-
A high-quality parsnip will be firm, reasonably atmosphere storage (Stoll and Weichmann 1987).
clean, and fairly smooth-surfaced (not deeply
ridged, no secondary rootlets). Parsnips are topped
after harvesting but should not be trimmed into the Retail Outlet Display Considerations
crown.
Water sprinklers and top-icing are acceptable for
nonpackaged product. Packaged products should
Horticultural Maturity Indices be held in a cold display case with no ice or water
sprinklers.
Parsnips are harvested when root diameter
is between 2.5 and 7.6 cm (1 and 3 in) at the
shoulder. Chilling Sensitivity
There are two grades, U.S. No. 1 and U.S. No. Ethylene Production and Sensitivity
2, based on shape, external appearance, and size.
Parsnips are commonly packaged in 11.4-kg (25- Parsnips produce very little ethylene: <0.1 µL
lb) perforated polyethylene bags or 5.5-kg (12-lb) kg-1 h-1 at 20 °C (68 °F). Exposure to low levels
cartons holding 12 cello bags of 0.5 kg (1 lb) each. of ethylene in cold storage causes bitterness,
likely due to accumulation of xanthotoxin
(8-methoxypsoralen) (Johnson et al. 1973,
Shattuck et al. 1988).
457
Respiration Rates Suitability as Fresh-Cut Product
458
Shattuck, V.I., R. Yada, and E.C. Lougheed. 1988.
Ethylene-induced bitterness in stored parsnips.
HortScience 23:912.
459
Passion Fruit Grades, Sizes, and Packaging
Robert E. Paull and Ching Cheng Chen Fruit should have a diameter of 5 to 8 cm (2 to
3 in) for purple and 6 to 8 cm (2.5 to 3 in) for
Paull and Chen are with the Department of yellow. Skin color should be full yellow or purple,
Tropical Plant and Soil Sciences, University of unless a hybrid. Fruit are packed in 6-kg (13.2-lb)
Hawaii at Manoa, Honolulu, HI. and 4.5-kg (9.9-lb) fiberboard cartons, sometimes
in one- or two-layer trays or cell packs.
460
Chilling Sensitivity 1978). This disease is most severe following
warm, wet periods. Septoria spot (Septoria
Symptoms of chilling injury on yellow passion passiflorae) infects fruit in the field and leads to
fruit are skin discoloration, pitting, water-soaked uneven ripening of the skin. Phytophthora fruit rot
areas, uneven ripening, and increased decay. (Phytophthora spp.) causes water-soaked, dark-
Discoloration can penetrate skin into the exocarp. green patches that dry up on the skin. Orchard
sanitation, reduction of high RH by pruning to
open the canopy, and application fungicides can
Ethylene Production and Sensitivity minimize these diseases.
461
on Postharvest Handling of Tropical Fruits, pp.
427-429. Chiang Mai, Thailand, July 1993. The
Australian Centre for International Agricultural
Research Proceedings no. 50, Canberra, Australia
462
Pea dirt, leaves or other foreign matter, mildew or
other diseases, insects, or mechanical or other
Stephen Morris and Jenny Jobling means. There are no USDA grades for snow or
snap peas, but best-quality snow peas are 7.6 to 9
Morris and Jobling are with the Sydney cm (3 to 3.5 in) long and 1.9 cm (0.75 in) wide,
Postharvest Laboratory, Food Science Australia, while snap peas should be 6.4 to 7.6 cm (2.5 to 3
North Ryde, New South Wales, Australia. in) long. Green peas are shipped in 13.6-kg (30-
lb) bushel baskets, or crates. Snow and sugar snap
peas are shipped in 4.5-kg (10-lb) cartons.
Specific Name and Introduction
There are three types of edible peas (Pisum Harvest Maturity Indices
sativum L.) from the family Fabaceae (also called
Leguminosae). The most common is the garden For best quality, both edible podded peas
or green pea P. sativum var. sativum L. This pea and green peas should be harvested before
has a tough pod that is discarded prior to eating physiological maturity; that is, before peas
(Basterrechea and Hicks 1991, Snowdon 1991). deform the hull (Basterrechea and Hicks 1991).
Most often these peas are frozen or processed. The Snow peas should be harvested when the pods
success of the frozen pea industry in the United are maximum size but before any visible seed
States has resulted in a decline in the sale of peas development; frequent harvesting is necessary.
sold in the pods (Basterrechea and Hicks 1991). Sugar snap peas should be harvested after they
The other two types of peas have soft, edible have developed seeds, as with garden peas
pods and belong to the subspecies P. sativum var. (Hocking 1997).
macrocarpon Ser. The snow or sugar pea has a flat
pod with minimal development of the seeds, while
the sugar snap pea or snap pea has well developed Precooling Conditions
seeds and is fully rounded (Hocking 1997, Suslow
and Cantwell 1998). The sugar snap pea is the Sugar content and flavor decrease rapidly after
result of a cross between the snow pea and an harvest unless green peas are promptly cooled to
unusual tightly podded pea with thick walls. All of 0 °C (32 °F). Therefore, they must be promptly
these peas grow best under cool, moist conditions. precooled after harvest by forced-air cooling,
hydrocooling, or vacuum-cooling. If vacuum-
cooling is used, it is important that the peas are
Quality Characteristics and Criteria prewet to ensure rapid cooling (Ryall and Lipton
1979). Forced-air cooling is the preferred method
Good-quality peas are uniformly bright green, of cooling for edible podded peas (NWREC
fully turgid, and free from defects and mechanical 1998).
damage. Stems and calyxes should be green.
Optimum Storage Conditions
Grades, Sizes, and Packaging
All three types of peas can be stored for 1 to 2
Grades include U.S. No. 1 and U.S. Fancy, based weeks at 0 °C (32 °F) with 95 to 98% RH (Wager
primarily on external appearance. They should 1964, Suslow and Cantwell 1998). Garden peas
exhibit similar varietal characteristics and be store better unshelled than shelled, possibly
not overmature or excessively small, not badly because shelling damages the peas (Basterrechea
misshapen or water-soaked, fairly well filled, and Hicks 1991). If there is surface moisture on
fresh, and free from decay and from damage peas, it is essential that they be stored below 2 °C
caused by black calyxes, freezing, splitting, hail, (36 °F).
463
Controlled Atmosphere (CA) To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
Considerations by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
Little work has been done on CA storage of peas. multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
Green pea quality was maintained better for 20 or by 61 to get kcal tonne-1 day-1.
days in 5 to 7% CO2 at 0 °C (32 °F) than in air
(Tomkins 1957). Snow and snap peas respond
moderately to CA of 2 to 3% O2 and 2 to 3% Physiological Disorders
CO2, though not all research has found CA to be
beneficial (Suslow and Cantwell 1998). Freezing may start at -0.6 °C (31 °F). Freezing
causes water-soaked areas followed by rapid
decay from soft rot bacteria (Suslow and Cantwell
Retail Outlet Display Considerations 1998). Edible podded peas are susceptible to
premature senescence resulting in yellowing, color
Storage with crushed ice can be beneficial, and changes in the calyx, and loss of tenderness and
water sprays are acceptable for garden and snow flavor (Suslow and Cantwell 1998). Storage at >5
peas, but these methods should be avoided for °C (41 °F) and exposure to ethylene can accelerate
snap peas (Suslow and Cantwell 1998). this problem.
Peas are not sensitive to low temperature and Because of high respiration rate, the heat
should be stored as close to 0 °C (32 °F) as generated by unrefrigerated peas will promote
possible without freezing. decay. Blemishes that reduce quality can be
caused by alternaria blight (Alternaria alternata),
anthracnose (Colletotrichum), ascochyta pod
Ethylene Production and Sensitivity spot (Ascochyta pisi Lib), and powdery mildew
(Erysiphe spp.) (Snowdon 1991). Common
Peas produce very low levels of ethylene: <0.1 diseases for edible podded peas are gray mold
μL kg-1 h-1 at 20 °C (68 °F). However, they are (Botrytis cinerea), watery soft rot (Sclerotinia
moderately sensitive to ethylene after harvest, sclerotiotorum), rhizopus rot, and bacterial soft
which results in yellowing and increased decay. rot. Botrytis gray mold can be a problem at the
The calyx is more sensitive to ethylene than the blossom-end of fresh-cut pods.
pod (Suslow and Cantwell 1998).
Quarantine Issues
Respiration Rates
There are no known quarantine issues.
Temperature Garden Peas Edible-pod
peas (estimated)
-----------mg CO2 kg-1 h-1----------- Suitability as Fresh-Cut Product
0 °C 30 to 46 30 to 48
5 °C 55 to 72 53 to 74 Edible podded peas are suitable for use in fresh-
10 °C 63 to 108 65 to 112 cut mixtures.
15 °C 165 to 185 165 to 187
20 °C 220 to 322 221 to 324
25 °C 298 to 327 —
Data from ASHRAE (1985) and Suslow and
Cantwell (1998).
464
Special Considerations
References
465
Peach Horticultural Maturity Indices
Carlos H. Crisosto and Adel A. Kader In California, harvest date of most cultivars is
determined by change in skin ground color from
Crisosto is with the Department of Plant Sciences, green to yellow. A color chip guide is used to
University of California, Davis, CA. Kader was determine maturity of each cultivar except white-
with the Department of Plant Sciences, University fleshed cultivars. A two-tier maturity system is
of California, Davis, CA. He is deceased. used in California: (1) U.S. Mature (minimum
maturity) and (2) Well-Mature or Tree Ripe. Well-
Mature and Tree Ripe are the same according
Scientific Name and Introduction to the California Department of Food and
Agriculture, Division of Inspection Services.
The peach, Prunus persicae, is native to China and
Persia (Iran); at one time it was called “Persian Measurement of fruit firmness is recommended
apple.” Chinese literature dates its cultivation in for cultivars in which skin ground color is masked
China to 1,000 B.C. Carried from China to Persia, by full red color development before maturation.
the peach quickly spread from there to Europe. In these cases, a maximum maturity index can
In the 16th century, it was established in Mexico, be applied. Maximum maturity is defined as
probably by the Spanish. Spanish missionaries the minimum flesh firmness (measured with a
introduced the peach to California in the 18th penetrometer with an 8-mm tip) at which fruit can
century. be handled without bruising damage. Bruising
susceptibility varies among cultivars.
California is a major producer and shipper of
peaches in the United States. The development of
white-fleshed peach cultivars is important. Current Grades, Sizes, and Packaging
fresh peach shipments approach 19 million 10-kg
(22-lb) packages from more than 155 cultivars. In Fruit are hand-picked into bags, baskets, or
the San Joaquin Valley, harvest of early cultivars totes. Fruit are then dumped into bins on trailers
starts in mid May, and harvest of late cultivars between tree rows in the orchard. If fruit are
is completed in early October. The United States picked into totes, the totes are usually placed
exports mainly to Canada, Taiwan, Hong Kong, directly inside the bins. Peaches are transported
Mexico, and South America. from orchard to packinghouse and cooled as soon
as possible after harvest.
Quality Characteristics and Criteria At the packinghouse, fruit are dumped (mostly
using dry bin dumps) and cleaned. Sorting is
There is high consumer acceptance of peaches done to eliminate fruit with visual defects and
with high SSC. TA and SSC:TA are also important sometimes to divert fruit of high surface color
factors in consumer acceptance. For mid-season to a high-quality pack. Attention to details in
peaches, a minimum of 11% SSC with TA ≤0.7% sorting line efficiency is especially important with
is required to satisfy about 80% of consumers. peaches, which have a range of colors, sizes, and
Fruit with 9 to 13.5 N (2 to 3 lb-force) flesh shapes. Sizing segregates fruit by either weight or
firmness are considered ready to eat. Fruit with dimensions.
firmness below about 30 N (7 lb-force) measured
on the fruit cheek have high consumer acceptance. Most yellow-flesh peaches are packed into 2-layer
(tray) boxes. Small yellow-flesh peaches are
generally volume-fill packed. Most white-flesh
and tree-ripe peaches are packed into 1-layer
(tray) boxes. Limited volumes of high-maturity
466
fruit are “ranch-packed” at the point of production. life, and shipping time. Large-sized ‘Elegant
In a typical tree-ripe operation, high-maturity or Lady’ and ‘O’Henry’ peaches stored at 0 °C
high-quality fruit are picked into buckets or totes benefit from an atmosphere of 6% O2 and 17%
that are carried by trailer to the packing area. CO2.
Packers work directly from buckets to select,
grade, size, and pack fruit into plastic trays. Fruit size, storage atmosphere, and temperature
all affect development of chilling injury. Small
peaches stored in air at 0 °C have a longer market
Precooling Conditions life than large fruit. At both temperatures, large-
sized ‘Elegant Lady’ and ‘O’Henry’ fruit have
Fruit can be cooled in field bins using forced-air a longer market life under CA than under air
cooling or hydrocooling. Forced-air cooling in storage. However, at 3 °C (38 ºF) small-sized
side-vented bins can be accomplished by either ‘Elegant Lady’ in CA showed flesh browning.
the tunnel or the serpentine method. Hydrocooling
is normally done by a conveyor-type hydrocooler
or in situ. Fruit in field bins can be cooled to Retail Outlet Display Considerations
intermediate temperatures of 5 to 10 °C (41 to 50
°F), provided packing will occur the next day. If If fruit firmness is <27 N (6 lb-force), fruit should
packing is to be delayed beyond the next day, fruit be displayed on a cold table. If firmness is >27
should be thoroughly cooled in the bins to near N (6 lb-force), fruit should be displayed on a dry
0 °C (32 ºF). In cultivars susceptible to internal table.
breakdown, fast cooling (within 8 h), as well as
maintaining fruit temperature near 0 °C (32 °F),
is recommended. Peaches in packed containers Chilling Sensitivity
should be cooled by forced-air cooling to near 0
°C (32 °F). Even peaches that were thoroughly Most mid-season and late peach cultivars
cooled in the bins will warm substantially during are susceptible to chilling injury or internal
packing and should be thoroughly recooled after breakdown. Chilling injury develops faster and
packing. more intensely in fruit stored at 2 to 7 °C (36 to 45
°F) than in those stored at 0 °C (32 °F) or below.
467
Respiration Rates occurs usually during harvesting and hauling,
though it may occur in other steps of postharvest
Temperature mg CO2 kg-1 h-1 handling. Gentle handling of fruit, short hauling,
0 °C 4 to 6 avoiding foliar nutrient sprays within 15 days of
10 °C 16 to 24 harvest, and following suggested guidelines for
20 °C 64 to 110 preharvest fungicide spray intervals will reduce
inking.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production, Postharvest Pathology
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1. Brown rot, caused by Monilinia fructicola, is the
most important postharvest disease of peaches.
Infection begins during flowering. Fruit rot may
Physiological Disorders occur before harvest but often appears after
harvest. Orchard sanitation to minimize infection
The major physiological cause of deterioration sources, preharvest fungicide application, and
is a low-temperature or chilling injury problem prompt cooling after harvest are among the control
generically called “internal breakdown” (IB). strategies. Also, postharvest fungicide treatment
The disorder can manifest itself as dry, mealy, may be used.
woolly, or hard-textured fruit; flesh or pit cavity
browning; or flesh translucency usually radiating Gray mold is caused by Botrytis cinerea. It can
through the flesh from the pit. An intense red be a serious problem during wet spring weather.
color development of the flesh (“bleeding”) It can occur during storage if fruit have been
usually radiating from the pit may be associated contaminated through harvest and handling
with this problem in some peach cultivars. In all wounds. Avoiding mechanical injuries and good
cases, flavor is lost before visual symptoms are temperature management are effective controls.
evident. However, there is large variability in IB
susceptibility among peach cultivars. In general, Rhizopus rot is caused by Rhizopus stolonifer. It
peach cultivars are more susceptible to IB than can occur in ripe or near-ripe peaches kept at 20
nectarine and plum cultivars. At the shipping to 25 °C (68 to 77 °F). Cooling and keeping fruit
point, fruit should be cooled and held near or below 5 °C (41 °F) is an effective control.
below 0 °C (32 °F). If IB-susceptible cultivars
are exposed to 5 °C (41 °F) during transportation,
their postharvest life can be significantly reduced. Quarantine Issues
Several treatments to delay and limit IB have
been tested. Among them, preripening fruit before Because some insects, such as Conotracherlus
storage is being used successfully in California. nenuphar (plum curculio), Cydia pomonella
The success of CA (17% CO2 and 6% O2) depends (codling moth), Rhagoletis pommonella
on cultivar market life, shipping time, and fruit (apple maggot), and Tetranychus pacificus are
size. not present in some of our import markets,
phytosanitary restrictions have been established.
Inking (black staining) is a cosmetic problem Issues associated with exotic pest quarantines,
affecting only the skin. This disorder is addressing either imported or exported peaches,
characterized by black-brown spots or stripes. can change rapidly. APHIS issues rules regarding
These symptoms appear generally 24 to 48 h import requirements. This agency provides
after harvest. Inking occurs as a result of abrasion information to assist exporters in targeting markets
damage in combination with contamination by and defining countries’ entry requirements.
heavy metals (iron, copper, and aluminum). It APHIS, in cooperation with State plant boards,
468
developed a database called “Excerpt” to track Suitability as Fresh-Cut Product
phytosanitary requirements for each country.
APHIS also provides phytosanitary inspections The optimal ripeness for preparing fresh-cut peach
and certifications that declare peaches free of pests slices is a flesh firmness of 13 to 27 N (3 to 6 lb-
to facilitate compliance with foreign regulatory force). These slices can be kept while retaining
requirements. good eating quality for 2 to 8 days (depending
on cultivar) at 5 °C (41 °F) with 90 to 95% RH.
For peaches, there are three main ways to deal Postcutting dips in ascorbate and calcium lactate,
with these phytosanitary requirements: inspection or use of MAP, may slightly prolong shelf-life.
prior to shipment (including use of screened
crates transported in sealed containers), methyl
bromide fumigation, and a systems approach. A Special Considerations
phytosanitary certificate (PC) is required to import
California peaches into Taiwan. Peaches must Because peaches area a climacteric fruit, they are
be free of Anarsia ineatella (peach twig borer), harvested after they reach a minimum maturity
Conotracherlus nenuphar (plum curculio), Cydia but before they are completely ripe (that is,
pomonella (codling moth), Erwinia amylovora ready to eat). Ripening must be initiated before
(fire bright), Rhagoletis pomonella (apple consumption in order to satisfy consumers.
maggot), Tetranychus pacificus (Pacific spider Detailed ripening protocols for shippers, retail
mite), and Ceratitis capitata (Mediterranean fruit handlers, warehouse managers, and produce
fly). If these conditions are not met, fruit must managers have been developed.
receive an appropriate treatment prior to shipment.
Details of the treatment must be recorded on the
PC. Acknowledgments
A phytosanitary certificate is required to import Some of the information in this chapter is from
California peaches into British Columbia. The PC the University of California, Davis, website on
should state that fruit are free of Cydia molesta “Produce Facts” at http://postharvest.ucdavis.edu/
(oriental fruit moth). Also, it should clearly produce_information.
state that fruit were produced and inspected in
accordance with the systems approach guidelines
agreed to by APHIS and the Canadian Food Further Reading
Inspection Agency. Fruit imports are unrestricted
to all other Canadian provinces. Ceponis, M.J., R.A. Cappellini, J.M. Wells, and
G.W. Lightner. 1987. Disorders in plum, peach
A similar program between APHIS and several and nectarine shipments to the New York market,
Mexican agencies exists to facilitate import 1972-1985. Plant Dis. 71:947-952.
and assure that peaches and nectarines are
free of Cydia molesta (oriental fruit moth), Crisosto, C.H. 1994. Stone fruit maturity indices:
Conotracherlus nenuphar (plum curculio), a descriptive review. Postharv. News Info.
Rhagoletis pomonella (apple maggot), and fruit 5(6):65N-68N.
flies (Tephritdae). Peaches imported into the
United States from other parts of the world are Crisosto, C.H., F.G. Mitchell, and S. Johnson.
sometimes fumigated with methyl bromide, at 1995. Factors in fresh market stone fruit quality.
shipping or arrival point, following treatment Postharv. News Info. 6(2):17N-21N.
schedules issued by APHIS, to prevent entry of
insect pests.
469
Crisosto, C.H., F.G. Mitchell, and Z. Ju. 1999.
Susceptibility to chilling injury of peach,
nectarine, and plum cultivars grown in California.
HortScience 34:1116-1118.
470
Pear cultivar. Pear fruit require a period of cold storage
at -1 °C (30 °F) to induce normal ripening and
develop high dessert quality upon ripening. When
Paul M. Chen
pear fruit meet the chilling requirement after a
period of cold storage, they should be ripened at
Chen is with the Mid-Columbia Agricultural
20 °C (68 °F) in air for 4 to 7 days depending on
Research and Extension Center, Oregon State
the variety for the development of high dessert
University, Hood River, OR
quality.
472
term high-CO2 treatment improves keeping quality duration at -1 °C (30 °F), which is required to
of ‘d’Anjou’ pears (Wang and Mellenthin 1975). induce normal ripening of pear fruit, is 2 to 4
Treatment with 12% CO2 for 2 weeks immediately weeks for ‘Bartlett’ (Puig et al. 1996, Agar et al.
after harvest has a beneficial effect on retention 1999), 2 to 3 weeks for ‘Bosc’ (Chen et al. 1982),
of ripening capacity. Keeping ‘d’Anjou’ pears in and 7 to 8 weeks for ‘d’Anjou’ (Chen et al. 1982).
a low-O2 atmosphere (1.0%) with ≤0.1% CO2 can
also maintain higher dessert quality and reduce Exogenous ethylene, at 100 µL L-1, has been
incidence of superficial scald after long-term cold used commercially to precondition underchilled
storage (Hansen 1957, Mellenthin et al 1980). ‘Bartlett’ and ‘d’Anjou’ pears at 20 °C (68 °F)
for 2 to 3 days before shipment. Preconditioned
pear fruit are capable of ripening normally and
Pear Ripening in Storage uniformly upon reaching the retail markets or for
the canning process (Chen et al. 1996, Puig et al.
Most pear cultivars require a period of cold 1996, Agar et al. 1999, 2000).
storage before they will ripen normally at room
temperature (Leblond and Ularic 1973, Drouet
and Hartmann 1979, Blankenship and Richardson Ethylene Production and Sensitivity
1985, Morin et al. 1985, Knee 1987). Most
cultivars do not soften appreciably during cold Ethylene production rates are low at harvest: <0.1
storage; hence, they require a period of ripening mL kg-1 h-1; but rates gradually increase during air
at warm temperature to develop good flavor and storage at -1.1°C (30 °F). After 3 mo of air storage
texture for eating. The best ripening temperature at -1.1 °C (30 °F) and 1 day at 20 °C (68 °F),
after storage is about 15 to 21 °C (59 to 70 ºF). ethylene production of ‘d’Anjou’ was 0.5 mL kg-1
Higher temperatures may result in poor quality h-1; of ‘Bosc,’ 30 mL kg-1 h-1; and of ‘Comice,’ 30
or decay. Most cultivars fail to soften at 30 °C mL kg-1 h-1.
(86 ºF). It is generally recognized that ethylene
induces ripening. Most pear cultivars will exhibit climacteric-
like ethylene production during the ripening
A striking characteristic of climacteric fruits period at 20 °C (68 °F) when optimally mature
is their autocatalytic ethylene production; that fruit have satisfied their chilling requirement
is to say, ethylene stimulates its own synthesis at -1.1 °C (30 °F). The chilling requirement of
(Pech et al. 1994). During storage at -1 °C (30 optimally mature pears stored at -1.1 °C (30
°F), 1-aminocyclopropane-1-carboxylic acid °F) for induction of normal ripening capacity is
(ACC; the immediate ethylene precursor) starts about 60 days for ‘d’Anjou,’ 30 days for ‘Bosc,’
to accumulate; as a consequence, pear fruit begin 30 days for ‘Comice,’ and 15 days for ‘Bartlett.’
to produce ethylene and ripen normally upon The magnitude of ethylene production at the
exposure to room temperature. Exposure of pear climacteric-like peak is 10 to 20 mL kg-1 h-1 for
fruit to a storage temperature of -1 to 0 °C (30 to ‘d’Anjou,’ 40 to 80 mL kg-1 h-1 for ‘Bosc,’ 80 to
32 ºF) stimulates synthesis of ACC and ethylene 200 mL kg-1 h-1 for ‘Bartlett,’ and 60 to 80 mL kg-1
because low temperature induces biosynthesis of h-1 for ‘Comice.’
ACC oxidase and ACC synthase (Blankenship
and Richardson 1985, Wang et al. 1985, Leliévre Unripe pear fruit are somewhat sensitive to
at al. 1997, Agar et al. 2000). The duration of ethylene, depending on the cultivar. ‘Bartlett’
chilling required for proper ripening is cultivar- pears are most sensitive to ethylene, ‘Bosc’ and
dependent (Wang et al. 1985) and varies with ‘Comice’ fruit are moderately sensitive, and
storage temperature (Sfakiotakis and Dilley 1974, ‘d’Anjou’ fruit are least sensitive to ethylene for
Gerasopoulos and Richardson 1995). Storage induction of ripening. Nevertheless, it is best to
473
avoid storing pear fruit near ripe fruit or other
fresh produce that produces appreciable levels of
ethylene. Preconditioning of pear fruit with 100
µL L-1 ethylene at 20 °C (68 °F) for 72, 48, or 24 h
will induce ripening within 3 to 6 days, depending
on the cultivar (Wang and Mellenthin 1972, Chen
et al. 1982, Elgar et. al. 1997).
Respiration Rates
‘d’Anjou’ ‘Bartlett’
Temperature Days in Storage Days in Storage
°C 1 25 50 100 150 200 1 10 20 90 120
-------------------------------------mg CO2 kg-1 h-1--------------------------------------
-2 5.0 2.0 2.0 2.3 2.4 2.7 — 1.5 2.2 3.2 3.8
-1 5.0 2.0 2.0 2.4 2.5 2.8 — 2.2 2.4 3.6 4.2
0 5.0 2.4 2.5 3.2 4.1 4.5 — 2.5 2.6 5.6 5.9
2 5.0 3.1 3.6 5.1 — — — 3.2 4.5 — —
10 9.0 6.0 7.0 — — — 16.0 8.1 24.0 — —
21 17.0 7.0 7.0 — — — 16.0 38.0 — — —
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and 1.8 at
20 °C (68 °F). To calculate heat production, multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 or by 61 to
get kcal tonne-1 day-1.
474
bins. Fruit at these contact points develop throughout the entire storage period to
pinkish (or blackened) ring markings during minimize development of black speck and
storage (Porritt et al. 1982), and the injured pithy brown core. An alternative method would
fruit become unmarketable. Ethoxyquin be to store field-run ‘d’Anjou’ pears in low O2
treatment as a line spray or a paper wrap (0.8% O2 and <0.03% CO2) at -1 °C (30 °F) for
is carried out during commercial packing. 90 days until qualified. Low-O2 stored fruit can
Because it usually takes about 3 mo to pack all be either presized or packed with in-line spray
fruit stored in air, fruit may receive ethoxyquin of 2,700 µL L-1 ethoxyquin within 2 weeks
protection as early as 1 week after harvest or after being returned to air storage. Treated fruit
as late as 3 mo after storage using either of can be further stored in regular CA storage
these methods. Though neither method causes (2% O2 and 1% CO2) for another 3 to 4 mo at
any phytotoxicity to fruit, delay in ethoxyquin -1 °C (30 °F) for late season marketing.
application has resulted in significant
incidence of scald disorder at terminal Pithy brown core (PBC) affects ‘d’Anjou’ pears
markets. Current recommended ethoxyquin stored in either sealed polyethylene box liners or
treatments are inadequate. CA storage with elevated CO2. It is characterized
by pithy, brown areas in the core region of the
Split application of ethoxyquin control method: fruit. It may be restricted to brown flecks between
Drenching ‘d’Anjou’ fruit with 1,000 µL the carpels, or it may encompass the entire core
L-1 ethoxyquin within 2 days after harvest and extend into the surrounding flesh. In some
controlled scald development for 4 mo in air instances, the tissues collapse, producing cavities.
storage without causing phytotoxicity to fruit. The affected tissues are dry and pithy, in contrast
Drenching fruit with 1,000 µL L-1 ethoxyquin to the soft, watery texture resulting from core
within 2 days after harvest plus an additional breakdown. The disorder is associated with
line spray of 1,700 µL L-1 ethoxyquin within 3 high CO2 in atmospheres of sealed box liners
mo of harvest controlled scald development for or CA storage and is considered to be a form of
5 mo in air storage (Chen et al. 1990a). CO2 injury. It is aggravated by the combination
of low O2 and high CO2 levels in CA storage.
Nonchemical control method: A short-term Susceptibility to PBC increases with factors that
(3- to 4-mo) CA storage of ‘d’Anjou’ pears at induce fruit senescence, such as late harvesting,
-1 ºC in 0.8% O2 and <0.1% CO2 (denoted as delayed storage, slow cooling, high storage
low O2) effectively controls development of temperatures, and extended CA storage (Hansen
superficial scald without inducing black speck and Mellenthin 1962). Fruit from trees with low
and pithy brown core (Chen and Varga 1997a, vigor and fruit grown in cool seasons are more
1997b). Fruit stored in a short-term low-O2 susceptible to PBC (Hansen and Mellenthin 1962).
regime remain free from scald after 8 weeks in
air at -1 ºC. These fruit can be safely packed For fruit stored loose in bins—field-run fruit—
without antioxidant treatment and marketed CO2 should be maintained at <0.03% when O2
after 2 mo of air storage. in CA storage is 0.8 to 1.0% (Chen and Varga
1997b). The maximum storage length of ‘d’Anjou’
Combined ethoxyquin/CA control method fruit stored under low O2 should be no more than
(Chen and Varga 1997a): For ‘d’Anjou’ fruit 120 days. For fruit stored loose in bins, CO2
destined for mid-term (5- to 6-mo) or long- should be maintained at <0.5% when O2 in CA
term (7- to 8-mo) CA storage, a prestorage storage is 1.5 to 1.8%. The maximum storage
drench of fruit with 1,000 µL L-1 ethoxyquin time of fruit stored in the CA regime described
within 2 days after harvest was necessary in above should be no longer than 8 mo. For fruit
order to control superficial scald disorder. The stored loose in bins, CO2 should be maintained at
concentrations of O2 and CO2 in CA storage <1.0% when O2 in CA storage 2.0 to 2.5%. The
must be kept at 1.5% and <0.5%, respectively, maximum storage time of fruit stored in the CA
475
regime described above should also be no longer final color of affected tissues is black, and cracks
than 8 mo (Chen and Varga 1997b). up to 3 cm in length may appear in the blackened
area. The affected area is usually confined to
For fruit stored in packed boxes, only the CA approximately 1 to 2 cm around the calyx, but it
regime with 2.0 to 2.5% O2 and <1.0% CO2 gives may cover the entire lower half of fruit in severe
minimal risk for ‘d’Anjou’ pears to develop PBC cases. Discoloration usually does not extend deep
after prolonged CA storage (6 to 8 mo) (Chen and into the flesh, sometimes only affecting the skin.
Varga 1997b).
Black end is believed to be caused by water
Black speck (BS), or “skin speckling,” of imbalance stemming from a restricted root
‘d’Anjou’ pears becomes an economic problem system. Excessive subsoil moisture in spring
after prolonged CA storage. It has been observed damages feeder roots, inducing development
frequently in commercial CA storage. It is a of the disorder. Fruit grown on trees with Asian
physiological disease and is suspected to be rootstocks (Pyrus serotina Rehder) are highly
closely associated with PBC. Kupferman (1988) susceptible to black end. Fruit grown on trees
reported that BS could develop on ‘d’Anjou’ with French rootstocks (Pyrus communis L.) are
pears with or without PBC. Similarly to PBC, generally free from the disorder (Heppner 1928,
‘d’Anjou’ fruit develop BS only in CA storage. Davis and Tufts 1932).
The unbalanced O2 :CO2 ratio in CA storage
must be an initial factor in causing skin injury of Core breakdown has been referred to in different
fruit. It was confirmed that BS was induced by investigations as “internal breakdown,” “core rot,”
≤1.0% O2 in CA storage for longer than 4 mo and “brown heart,” and “mealy core.” As the names
was not affected by <1% CO2 (Chen and Varga imply, this disorder of pears is characterized
1989). Each BS spot consists of several hundred by the softening and browning of tissues in the
epidermal cells discolored to dark brown. The region of the core. The breakdown may be closely
distinct specks scatter randomly on the peel tissue confined to the core or may extend into the
and are not restricted to around the lenticels. It surrounding flesh. In the early stages, the affected
affects only five to six layers of hypodermal cells tissues are soft and watery; they later become dark
of the skin (Kupferman 1988, Chen and Varga brown. The disorder is often associated with an
1989, Lee et al. 1990). external skin discoloration resembling senescent
scald.
Since BS is associated with low O2 (≤1%), a CA
regime with O2 at 1.5% or higher will alleviate Overmature fruit are more susceptible to core
the disorder after prolonged CA storage. For breakdown in storage and in the market. Late-
‘d’Anjou’ pears, low-O2 (0.8 to 1.0% O2) storage harvested pears are unable to slow their metabolic
should not exceed 120 days (Chen and Varga activities sufficiently for successful storage at
1997b). -1 °C (30 ºF). Serious breakdown can occur in
storage or after pears are removed to ripen at 20
Black end of ‘d’Anjou’ pears begins with °C (68 ºF). Late-harvested pears show an increase
symptoms appearing when fruit are one-third in CO2 in the tissues around the core area until
to one-half full size. It is a protrusion of the the onset of core breakdown. Such fruit produce
calyx due to the retarded development of tissues an abnormally high amount of acetaldehyde until
around the calyx. As the disorder progresses, the core breakdown is advanced. ‘Bartlett,’ ‘Bosc,’
calyx lobes turn black, the tissues surrounding and ‘Comice’ pears are highly susceptible to
the calyx opening become hard, and a brownish core breakdown, whereas ‘d’Anjou’ pears are
discoloration forms. This discoloration may somewhat resistant. Harvesting fruit at optimum
appear first in separate spots, which later coalesce; maturity and rapidly precooling fruit before
or there may be a large area completely and storage are essential to reduce core breakdown
uniformly discolored from the beginning. The (Hartman 1925, Harley 1929).
476
Senescent scald occurs differently in various margin of a decayed area and plating some of the
pears. Pears can be classified broadly into two decayed flesh on potato-dextrose agar.
groups based on their storage behavior. One
group, including ‘d’Anjou,’ ‘Winter Nelis,’ Fungi causing postharvest decay in pome fruits
‘Packham’s Triumph,‘ and ’Hardy,‘ senesce can be subdivided into two broad groups: those
very slowly in cold storage and generally do not that cause primarily postharvest decay and those
lose their capacity for normal ripening at room that also cause fruit decay in the field. Postharvest
temperature as a result of extended storage. These decay attributable to fungi in the latter group
pears are subject to storage scald, called “Anjou usually result from infections that occurred in
scald” or “superficial scald.” The other group of the field but were quiescent or otherwise escaped
pears, which includes ‘Bartlett,’ ‘Bosc,’ ‘Howell,’ notice at harvest. Control measures for these
‘Comice,’ ‘Sierra,’ and ‘Flemish Beauty,’ show diseases are based on the protection of fruit from
a higher rate of senescence during cold storage; preharvest infections. Control measures broadly
fruit lose their ability for normal ripening at room applicable to many postharvest diseases include
temperature after prolonged storage. As storage both sanitation and harvesting, as well as handling
length is extended, fruit become yellow and methods that maintain the integrity of harvested
ultimately develop a dark brown discoloration of fruit. Sanitation measures are especially important
the skin called “senescent scald.” This may occur for reducing exposure of fruit to spores of wound
in cold storage or after fruit are removed from pathogens, such as Penicillium and Botrytis.
cold storage and subjected to higher temperatures. Harvesting and handling methods are important
Fruit remain firm, but the skin sloughs off easily because wounded, bruised, or overmature fruit
(Porritt et al. 1982). are more susceptible to many postharvest decay
fungi. Rapid cooling after harvest reduces the
Methods of treating senescent scald are similar incidence of decay in storage, because low
to those used to reduce core breakdown disorder, temperatures reduce both the rate of fungal growth
with overmature fruit more susceptible in storage and fruit senescence. Low temperature prevents
and in the market. Pears that are harvested late development of incipient infections by some fungi
do not exhibit a decrease in metabolic activity in storage, such as those causing white rot and
necessary for storage at -1 °C (30 ºF). There are brown rot.
no control measures other than harvesting pears
at optimum maturity, reducing the storage period, Postharvest decay pathogens infecting pears in
and improving postharvest handling with methods the field and causing symptoms in stored pears
such as fast cooling of pears immediately after as a result of incipient or quiescent infections
harvest and maintaining constant temperature at -1 are Botryosphaeria dothidea (white rot), B.
°C (30 ºF) throughout storage (Porritt et al. 1982). obtusa (black rot), Glomerella cingulata (bitter
rot), Monilinia fructicola (brown rot), M.
fructigena (brown rot), M. laxa (brown rot), and
Postharvest Pathology Phytophthora cactorum (phytophthora fruit rot).
A detailed description of pear diseases and control Postharvest diseases commonly found in pears
measures can be found in “Compendium of Apple after storage are blue mold (Penicillium spp.),
and Pear Diseases” (Jones and Aldwinckle 1990). gray mold (Botrytis cuberea Per.), bull’s-eye
The cause of postharvest decay in pear fruit is rot (Pezicula malicorticis [H. Jacks.] Nannf.),
often difficult to identify through symptoms alone. alternaria rot (Alternaria alternata [Fr.] Keissler),
Many decay pathogens do not sporulate on fruit mucor rot (Mucor piriformis E. Fischer), side
in cold or CA storage. The only reliable method rot (Phialophora malorum [Kidd & Beaumont]
for identifying the cause of decay is to isolate McColloch), cladosporium rot (Cladosporium
and identify the causal agent. Isolations are easily herbarum [Pers.] Link), coprinus rot (Coprinus
accomplished by removing the fruit skin over the psychromorbidus Redhead & Traquair), fisheye rot
477
(Butlerelfia eustacei Weresub & Illman), pink rot Chen, P. M., and D.M. Varga. 1997b.
(Trichothecium roseum [Pers.] Link), and rhizopus Determination of optimum controlled atmosphere
rot (Rhizopus stolonifer [Ehrenb.] Vuill.). regimes for the control of physiological disorders
of ‘d’Anjou’ pears after short-term, mid-term
or long-term storage. In E.J. Mitcham, ed., 7th
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Chen, P.M., D. Varga, E. Mielke, et al. 1990b.
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Development of ethylene biosynthesis and by ethoxyquin: oxidation of α-farnesene and its
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Hansen, E. 1964. Control of anjou pear scald reported. Good Fruit Grower 39(16):17-18.
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l’evolution de la maturation par divers traitements
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influencing susceptibility of pears to carbon Crassane.” Bull. Inst. Intl. Froid. 3:69-74.
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Hansen, E., and W.M. Mellenthin. 1967. Chemical the skin tissues of normal and black-speckled
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‘Bartlett’ pears. J. Agric. Res. 39:483-493. prevention, and nitrogen metabolism of ‘d’Anjou’
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discoloration of ‘d’Anjou’ pears in relation to fruit
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Heppner, M.J. 1928. Pear black-end and its le metabolisme de l’ethylene au cours de la
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480
Pepper of which depends on the pepper cultivars used.
For instance, cayenne powder is a high-pungency
Gustavo Adolfo González-Aguilar condiment produced from dried mature fruit of
cayenne-type cultivars
González-Aguilar is with the Centro de
Investigación en Alimentación y Desarrollo,
Hermosillo, Sonora, Mexico. Quality Characteristics and Criteria
481
15.9 kg (15 to 35 lb) of randomly packed peppers. ripening and respiration during transit and storage
Very high quality peppers are often marketed in and benefit quality slightly. At 10 °C (50 °F),
5-kg (11-lb) flat cartons with one or two layers of high CO2 (>5%) can cause calyx discoloration,
fruit. skin pitting, and discoloration and softening in
both bell and chili peppers. An atmosphere of
There are no U.S. grades for chili peppers. 3% O2 and 5% CO2 is more beneficial for red
than for green peppers stored at 5 to 10 °C (41
to 50 °F) for 3 to 4 weeks. Before processing,
Precooling Conditions chili peppers can be stored under 3 to 5% O2 and
15 to 20% CO2 for up to 3 weeks at 5 °C (41 °F)
After harvest, fresh market peppers should be without appreciable chilling injury or quality loss.
rapidly cooled to no lower than 7 °C (45 °F) Freshly harvested chili or other hot peppers should
at high RH to reduce water loss and shrivel. be stored under the same temperature and RH
Precooling can be done using forced-air cooling, conditions as sweet peppers.
hydrocooling, or vacuum-cooling. Properly
vented cartons are required to facilitate forced-
air cooling. If hydrocooling is used, care should Retail Outlet Display Considerations
be taken to prevent development of decay. High
RH is necessary to avoid desiccation. Waxing has Fresh bell and chili peppers should be displayed at
been used to reduce desiccation, but it tends to 7 °C (45 °F). They should not be sprinkled or top
increase bacterial soft rot. Shelf-life varies among iced. Dried chili peppers should be kept dry.
different pod types. Deterioration is often due to
moisture loss, with some pod types more prone to
desiccation than others. Chilling Sensitivity
482
Respiration Rates Quarantine Issues
Blossom end rot is characterized by a slightly Mechanically harvested peppers are usually
discolored or dark sunken lesion at the blossom unsuitable for fresh market because of extensive
end of the fruit. It is caused by calcium deficiency injuries but can be used for processing. Peppers
during growth. Pepper speck appears as spotlike must be handled with care to avoid mechanical
lesions that penetrate the fruit wall. The cause is damage that may cause discoloration and
unknown; some varieties are more susceptible. pathological problems. Before packaging, peppers
Chilling injury is described above. can be washed with 300 µL L-1 chlorine to reduce
disease. Waxing with fungicides reduces water
loss and disease.
Postharvest Pathology
After drying, chili peppers are packaged tightly
The most common decay microorganisms are into sacks holding ≥200 lb (≥91 kg) and are
Botrytis, Alternaria, and soft rots of fungal generally stored in nonrefrigerated warehouses
and bacterial origin. Botrytis (grey mold) is a for up to 6 mo. The temperature of the warehouse
common organism on peppers. Field sanitation depends on its construction and the way it
and prevention of wounds on the fruit reduce is managed, but mainly on ambient outdoor
its incidence. Botrytis grows well at the temperature. Insect infestation is a major storage
recommended pepper storage temperatures. problem. In Southern States, chili and other hot
High CO2 levels (>10%) can control Botrytis peppers are dried, packaged, and stored at 0 to
but damages peppers. Hot water dips at 53 to 10 °C (32 to 50 °F). Storage at low temperature
55 °C (126 to 130 °F) for 4 min can effectively retards loss of red color and slows insect activity.
control botrytis rot without causing fruit injury.
The presence of alternaria black rot, especially Moisture content of chili and other hot peppers
on the stem end, is a symptom of chilling injury. during storage should be low (10 to 15%) to
The best control is to store peppers at 7 °C prevent mold growth. A RH of 60 to 70% is
(45 °F). Bacterial soft rot is caused by several desirable. With a high moisture content, pods
bacteria that attack damaged tissue. Soft rots can may be too pliable for grinding and may have
occur on washed or hydrocooled peppers when to be redried. With lower moisture content
water sanitation is inadequate. Peppers are also (<10%), pods may be so brittle they shatter during
affected by many of the disease, virus, insect, and handling, causing loss and release of dust, which
nematode pests that affect tomato. is irritating to the skin and respiratory system.
483
The use of polyethylene film liners within bags Cantwell, M. 1998. Bell peppers.
allows better storage and reduces dust. The liners Recommendations for maintaining postharvest
ensure that the pods maintain constant moisture quality. University of California, Davis, CA.
content during storage until the time of grinding. At http://postharvest.ucdavis.edu/produce_
Thus, they permit successful storage or shipment information.
under a wide RH range. Peppers can be stored 6 to
9 mo at 0 to 4 °C when packed in this manner. Gonzalez-Aguilar, G.A., and M. Tiznado. 1993.
Postharvest physiology of bell peppers stored in
Manufacturers of hot-pepper products hold part of low density polyethylene bags. Lebensm-Wiss.
their raw material in cold storage at 0 to 10 °C but U-Thechnol. 26(5):450-455.
prefer to grind peppers immediately and store the
dried product in air-tight containers. Hardenburg, R.E., A.E. Watada, and C.Y.
Wang. 1986. The Commercial Storage of Fruits,
Whole peppers are also dried until they are brittle Vegetables, and Florist and Nursery Stocks.
and the seeds and pulp are completely dry. The Agricultural Handbook 66, U.S. Department of
dried product is used in flavoring and improving Agriculture, Washington, DC.
the appearance of various products, including
canned products. Some sliced peppers are partially Miller, W.R., and L.A. Risse. 1986. Film wrapping
dried and mixed with salt for preservation for to alleviate chilling injury of bell peppers during
ultimate use in various processed products. cold storage. HortScience. 21:467-468.
484
Perennial Culinary Herbs Precooling Conditions
Scientific Names and Introduction Chives and mint can be stored for 2 to 3 weeks at
0 °C (32 °F) with 95 to 100% RH; Chinese chives
Perennial culinary herbs include chives (Allium last for 1 to 2 weeks. Top icing is sometimes used
schoenoprasum L.), Chinese chives (A. tuberosum for mint. Marjoram, oregano, and tarragon can be
Rottler), marjoram (Origanum hortensis), oregano stored at 0 °C (32 °F) with 90 to 95% RH for 1 to
(O. vulgare L.), peppermint (Mentha piperita L.), 2 weeks; rosemary, sage, and thyme will last for
spearmint (M. spicata L.), rosemary (Rosmarinus 2 to 3 weeks (Hruschka and Wang 1979, Cantwell
officinalis L.), sage (Salvia officinalis L.), tarragon 1997).
(Artemesia dracunculus L.), and thyme (Thymus
vulgaris L.). The leaves are the part primarily used
in foods and cooking. Due to their strong flavors Controlled Atmosphere (CA) and Modified
and aromas, these culinary herbs are generally Atmosphere Packaging Considerations
used in small quantities.
Because of their short postharvest life, CA is
generally not used for fresh herbs. MAP has been
Quality Characteristics and Criteria shown to retard yellowing in chives (Aharoni et al.
1993).
Herbs should appear fresh and green, with no
yellowing, decay, insect damage, or mechanical
damage. Leaves should be uniform in size. Flavor Retail Outlet Display Considerations
and aroma should be strong and characteristic of
the herb. Use of water sprinklers is acceptable.
Most herbs for fresh culinary use are best if These perennial herbs are not chilling sensitive
harvested before flowering. Exceptions are and should be stored as close to 0 °C (32 °F) as
marjoram and oregano (sometimes sold with possible without freezing.
flower buds) and chive blossoms (sometimes used
in salads or as edible garnishes).
Ethylene Production and Sensitivity
485
Respiration Rates Suitability as Fresh-Cut Product
Quarantine Issues
486
Persimmon Horticultural Maturity Indices
487
and 59 ºF); they will exhibit flesh browning persimmons are higher than those of nonchilled
and softening. Exposure to ethylene aggravates fruits. Exposure to ethylene at 0.1 µL L-1 or
chilling injury at these temperatures. higher aggravates chilling symptoms of ‘Fuyu’
persimmons, while CA ameliorates symptoms.
Chilling injury is controlled by avoiding exposure
Ethylene Production and Sensitivity of ‘Fuyu’ fruit to temperatures between 2 °C (36
°F) and 15 °C (59 °F). The optimum storage and
Persimmons produce <0.1 µL kg-1 h-1 at 0 °C (32 transport temperature is 0 °C (32 °F). Exposure
°F) and 0.1 to 0.5 µL kg-1 h-1 at 20 °C (68 °F). to ethylene >1 µL L-1 during postharvest handling
They are very sensitive to ethylene. Exposure to 1 should also be avoided. CA of 3 to 5% O2 and 5 to
and 10 µL L-1 ethylene at 20 °C (68 °F) accelerates 8% CO2 at temperatures <5 °C (<41 °F) reduces
softening to less than 4 lb-force (17.8 N), the limit chilling injury.
of marketability, after 6 and 2 days, respectively.
Thus, removing or excluding ethylene from Exposure to O2 levels <3% during storage for
transport and storage facilities is recommended. longer than 1 mo can result in failure of fruit to
ripen and development of off flavors. Exposure to
CO2 >10% during storage for longer than 1 mo
Respiration Rates can cause brown discoloration of the flesh and
lead to development of off flavors.
Temperature mg CO2 kg-1 h-1
0 °C 4 to 8 Calyx separation is a problem with some
20 °C 20 to 24 cultivars. It has caused losses in New Zealand.
Growing conditions are all-important, and
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate excessive nitrogen fertilization should be avoided.
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and Thinning trees early in the season will enhance
1.8 at 20 °C (68 °F). To calculate heat production, calyx growth and help prevent this disorder.
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
Postharvest Pathology
488
assist exporters in targeting markets and defining References
what entry requirements a particular country has.
APHIS, in cooperation with State plant boards, Wright, K.P., and A.A. Kader. 1997. Effect of
developed a database called “Excerpt” to track slicing and controlled-atmosphere storage on the
phytosanitary requirements for each country. ascorbate content and quality of strawberries and
APHIS also provides phytosanitary inspections persimmons. Postharv. Biol. Technol. 10:39-48.
and certifications that declare fruit are free of pests
to facilitate compliance with foreign regulatory
requirements. Further Reading
489
Prusky, D., R. Ben-Arie, and S. Guelfat-Reich.
1981. Etiology and histology of Alternaria rot of
persimmon fruits. Phytopathology 71:1124-1128.
490
Pineapple less mature fruit are selected for this purpose
(Akamine 1963, Cancel 1974). Immature fruit
Robert E. Paull and Ching Cheng Chen should not be shipped, since they do not develop
good flavor, have low Brix, and are more prone to
Paull and Chen are with the Department of Plant chilling injury (Rohrbach and Paull 1982).
and Soil Sciences, University of Hawaii at Manoa,
Honolulu, HI.
Grades, Sizes, and Packaging
491
(Paull 1993). RH of 85 to 95% is recommended; Chilling Sensitivity
high RH significantly reduces water loss. Ripe
fruit can be held at 7 °C (45 °F) for about 7 to 10 Symptoms of chilling injury include wilting,
days. Pineapples may be stored at 0 to 4 °C (32 drying and discoloration of crown leaves, failure
to 39 °F) for weeks, but upon removal fruit fail of green-shelled fruit to yellow, browning and
to continue ripening and show severe chilling dulling of yellow fruit, and internal flesh browning
injury. Quarter-yellow fruit at harvest gain about 1 (Lim 1985, Paull and Rohrbach 1985). Preharvest
additional week of storage for every 6 °C (11 °F) shading, as well as preharvest and postharvest
decrease in storage temperature (Dull 1971). The low temperature, are the major factors increasing
maximum storage life at 7 °C (45 °F) is about 4 symptom intensity (Akamine et al. 1975, Akamine
weeks (Paull and Rohrbach 1985); however, when 1976, Keetch and Balldorf 1979, Smith 1983).
the fruit are removed, chilling-injury-induced Chilling injury symptoms include endogenous
internal browning develops within 2 to 3 days. brown spot, physiological breakdown, blackheart,
and internal browning. Symptoms develop after
fruit are returned to physiological temperatures
Controlled Atmospheres (CA) of 15 to 30 °C (59 to 86 °F) (Paull and Rohrbach
Consideration 1985). Susceptible fruit are generally lower in
ascorbate and sugar and are opaque (Abdullah
Modifying O2 levels is only minimally effective and Rohaya 1983, Abdullah et al. 1985, Paull and
at extending pineapple shelf-life (Dull et al. 1967, Rohrbach 1985, Swete Kelly and Bragshaw 1993).
Akamine and Goo 1971). Some benefit is gained
from CA (4% O2) treatments in reducing chilling
injury development (Paull and Rohrbach 1982). Ethylene Production and Sensitivity
The fruit waxes currently used generate high
internal concentrations of CO2 (up to 5%) and The ethylene production rate of this
reduced O2 (Paull and Rohrbach 1982). Low O2 nonclimacteric fruit is low: 0.1 to 1.0 µL kg-1 h-1.
has no effect on crown condition or decay but Postharvest use of ethephon to degreen the shell
does delay shell color development and reduce has been tested in the Ivory Coast (Poignant 1971,
superficial mold growth (Akamine and Goo 1971). Crochon et al. 1981), Hawaii (Paull 1985), and
Subjecting fruit to 1 to 2% O2 and 0 to 10% CO2 Australia (Smith 1991). Treated fruit show more
after shipment from Mexico to England at 8 ºC did rapid uniform skin degreening and little change in
delay chilling injury (Haruenkit and Thompson quality. However, shelf-life is slightly shortened
1994). Polyethylene bagging, though difficult to (Paull 1985, unpublished data; Smith 1991, Soler
perform commercially on individual fruit, results 1992a). There is no recommendation for use of
in atmosphere of 8 to 10% O2 and 7% CO2, and ethephon; it is not approved for postharvest use.
fruit waxing delays appearance of chilling-induced
internal browning (Paull and Rohrbach 1982,
Rohrbach and Paull 1982, Abdullah et al. 1985). Respiration Rates
The tentative recommendation is 2 to 5% and 5 to
10% CO2 (Yahia 1998). Temperature mg CO2 kg-1 h-1
5 °C 2
10 °C 4 to 7
Retail Outlet Display Considerations 15 °C 10 to 16
20 °C 19 to 29
Whole fruit should be displayed refrigerated at 10
to 13 °C (50 to 55 °F) and should not be misted or To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
iced. by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
492
Physiological Disorders Sacc. Red Spanish types are more resistant than
‘Smooth Cayenne.’ Infection occurs within 8 to
Flesh translucency, also called porosity, is 12 h following harvest and enters through the
associated with greater fruit sensitivity to point of detachment or wounds. The severity of
mechanical injury, indicated by leakage and the problem depends on the degree of bruising or
oozing of cellular fluids. This condition begins wounding during harvesting and packing, the level
before harvest and continues after (Bowden of inoculum on the fruit, and storage temperature
1969, Rohrbach and Paull 1982, Paull and Reyes during transportation and marketing (Rohrbach
1996). Fruit with increased translucency also have and Schmitt 1994). The rot is commercially
increased pH, a higher SSC:acid ratio, higher controlled by minimizing bruising of fruit during
fruit weight, higher total esters, and lower acidity. harvest and handling, refrigerating the fruit, and
SSC, flesh pigments, and palatability increase to a applying postharvest fungicides (Rohrbach and
maximum at about 60% translucency, then decline Phillips 1990).
in fruit with higher translucency (Bowden 1969).
Fruitlet core rot, black spot, fruitlet brown rot,
Bruising by impact damage is a major problem and eye rot describe the brown to black color of
during harvesting, packing, and shipping of the central part of an individual fruitlet. Epidemic
pineapple. Mechanical injury of translucent fruit levels are rare in the major commercial pineapple-
can lead to leakage of cell contents and loss of producing areas of the world (Rohrbach and
marketable fruit. Injury can be avoided by careful Schmitt 1994). Low-acid cultivars being grown
handling and avoiding impacts and bruising. commercially are most susceptible (Rohrbach
and Schmitt 1994). This disease is caused by a
Sunburned, or sun-scorched, pineapples show complex of fungi (Rohrbach and Schmitt 1994).
a bleached, yellow-white skin that turns pale Infection frequently can lead to misshapen fruit
gray-brown with damage to the flesh underneath. that are culled before packing and shipping.
Damaged areas are more susceptible to disease.
It is common during hot periods (>35 °C [95 ºF]) Yeasty fermentation arises due to the fact that
(Keetch and Balldorf 1979). fruit are not sterile inside, containing many
nongrowing but viable yeasts and bacteria. In
Malformations, or pineapple fruit with pronounced damaged, overripe fruit and fruit with interfruitlet
eyes or fruitlets, are normally not acceptable in cracking, resident yeasts begin to grow or new
Fancy grades of fruit, and the thicker skin results yeasts invade. This growth leads to fermentation
in lower flesh recovery. This condition is common and bubbles of gas and juice through cracks in the
in fruits that flower during cool weather. Some skin. The skin turns brown and leathery and fruit
Spanish varieties are susceptible to broken core, become spongy with bright yellow flesh.
in which the central core has a transverse break
leading to the upper part of the fruit ripening Saprophytes (Penicillium sp.) growing on the
ahead of the bottom (Lim 1985). broken end of the peduncle and fruit surface are
nonpathogenic but are unsightly and therefore
a marketing problem (Rohrbach 1989). The
Postharvest Pathology condition is more common on highly translucent
fruit.
Black rot, also called thielaviopsis fruit rot, water
blister, soft rot, or water rot, is a universal fresh
pineapple problem characterized by a soft watery Quarantine Issues
rot (Rohrbach 1983). Diseased tissue turns dark
in the later stages of the disease because of the Pineapple fruit that are >50% ‘Smooth Cayenne’
dark mycelium and spores. Black rot is caused are not regarded a host for tephritid fruit flies.
by the fungus Chalara paradoxa (De Seynes)
493
Thus, insect disinfestation is not required for Suitability as Fresh-Cut Product
import into fly-free countries (Armstrong 1994).
Fresh-cut pineapple is readily prepared at a
Pineapple caterpillars (Thecla basilides Geyer, central plant or in-store for ready-to-use consumer
Metamasius ritchiei Marchall, Batrachedre pack. Packs contain fresh-cut cylinders with the
methesoni Busch, and Paradiophorus crenatus core removed or spears, chunks, or wedges. The
Billbarg) are exotic and limited to Central product has a shelf-life of at least 7 days at proper
America, South America, and the Caribbean temperature. A patent has been issued for the
(Harris 1927, Rohrbach 1983). The adult oviposits use of pouches flushed with 15 to 20% O2 and
on the inflorescence prior to anthesis. Larvae then 3% argon and held at 1 °C (34 °F) for 10 weeks
infest fleshy parts of the bracts and feed inside (Powrie et al. 1990) for fresh-cut pineapple pieces.
the developing inflorescence, exuding gum from
the feeding chambers. Control with insecticides is
relatively easy if flowering is induced uniformly Special Considerations
with forcing agents. T. basilides is a tropical
species that could cause problems if imported Ease of removal of crown leaves, full skin
into Southern States such as Florida, as it can yellowing, or the sound produced by tapping the
feed on corn, cacao, heliconia, and several other fruit are not signs of ripeness or quality. Fruit are
bromeliads (Rohrbach 1983). picked at the ripe stage and are ready-to-eat, even
if there is a little skin yellowing.
Pineapple scale, Diaspis bromeliea Kerner, occurs
wherever pineapple is grown. Normally, in Hawaii
pineapple scale is not a major problem in fields, References
probably because of scale parasites and predators.
However, because of the quarantine requirement Abdullah, H., and M.A. Rohaya. 1983. The
to have fruit insect-free, even low levels of development of black heart disease in Mauritius
pineapple scale at harvest present quarantine pineapple (Ananas comosus cv. Mauritius) during
problems. Scale are controlled by preharvest storage at lower temperatures. MARDI Res. Bull.
insecticide applications, taking into account “last- 11:309-319.
application-to-harvest” time.
Abdullah, H., M.A. Rohaya, and M.Z. Zaipun.
The pineapple fruit mite, Steneotarsonemus 1985. Effect of modified atmosphere on black
ananas Tryon, occurs universally on the growing heart development and ascorbic acid contents
plant, developing in the inflorescence, fruit, and in ‘Mauritius’ pineapple (Ananas comosus cv.
crown. Fruit mites feed on developing trichomes ‘Mauritius’) during storage at low temperature.
on the white basal leaf tissue and flower bracts and ASEAN Food J. 1:15-18.
sepals, causing light brown necrotic areas. The
pineapple red mite, Dolichotetranychus floridanus Akamine, E.K. 1963. Fresh pineapple storage.
Banks, feeds on the white basal leaf tissue, Hawaii Farm Sci. 12:1-4.
particularly of the crown. Severe damage occurs
when the fruit mature under drought conditions. Akamine, E.K. 1976. Postharvest control of
Red mites may cause death of basal crown leaves, endogenous brown spot in fresh Australian
affecting quality (Rohrbach and Schmitt 1994). pineapples with heat. HortScience 11:586-588.
Mealybugs are removed from the surface by Akamine, E.K., and T. Goo. 1971. Controlled
brushing. Preharvest insecticide and ant control atmosphere storage of fresh pineapples (Ananas
almost eliminate mealybugs (Soler 1992b). comosus [L.] Merr. ‘Smooth Cayenne’). Res. Bull.
Crickets and locusts may feed on bracts before 152, Hawaii Agricultural Experiment Station,
harvest. Honolulu, HI.
494
Akamine, E.K., T. Goo, T. Steepy, et al. 1975. Hawaii Department of Agriculture. 1968.
Control of endogenous brown spot of pineapple Wholesale standards for Hawaiian-grown
in postharvest handling. J. Amer. Soc. Hort. Sci. pineapple. Hawaii Department of Agriculture,
100:60-65. Marketing Division, Honolulu, HI.
Armstrong, J.W. 1994. Tropical and subtropical Keetch, D.P., and D. B. Balldorf. 1979. The
fruits. In R.E. Paull and J.W. Armstrong, eds., incidence of certain pineapple fruit blemishes in
Insect Pests and Fresh Horticultural Products: the eastern cape and border. Citrus Subtrop. Fruit
Treatments and Responses, pp. 275-290. CAB J. 551:12-15.
Intl., Wallingford, U.K.
Lim, W.H. 1985. Diseases and Disorders of
Bowden, R.P. 1969. Further studies on ripeness in Pineapples in Peninsular Malaysia. Report 97,
pineapple. Food Tech. Australia. 21:160-163. MARDI, Kuala Lumpur, Malaysia.
Cancel, H.L. 1974. Harvesting and storage Nakasone, H.Y., and R.E. Paull. 1998. Tropical
conditions for pineapples of the ‘Red Spanish’ Fruits. CAB Intl., Wallingford, U.K.
variety. J. Agric., Puerto Rico 58:162-169.
Paull, R.E. 1993. Pineapple and papaya. In
Collins, J.L. 1968. The Pineapple. Leonard Hill, G. Seymour, J. Taylor, and G. Tucker, eds.,
London, U.K. Biochemistry of Fruit Ripening, pp. 291-323.
Chapman & Hall, London, U.K.
Crochon, M., R. Tisseau, C. Teisson, and R. Huet.
1981. Effet d’une application d’Ethrel avant la Paull, R.E., and K.G. Rohrbach. 1982. Juice
recolte sur la qualite gustative de ananas de Cote characteristics and internal atmosphere of waxed
d’Ivoire. Fruits 36:409-415. ‘Smooth Cayenne,’ pineapple fruit. J. Amer. Soc.
Hort. Sci. 107:448-452.
Dull, G.G. 1971. The pineapple: general. In A.C.
Hulme, ed., The Biochemistry of Fruits and Their Paull, R.E., and K.G. Rohrbach. 1985. Symptom
Products, vol. 2, pp. 303-331. Academic Press, development of chilling injury in pineapple fruit.
London, U.K. J. Amer. Soc. Hort. Sci. 110:100-105.
Dull, G.C., R.E. Young, and J.B. Biale. 1967. Paull, R.E., and M.E.Q. Reyes. 1996. Preharvest
Respiratory patterns in fruit of pineapple, weather conditions and pineapple fruit
Ananas comosus, detached at different stages of translucency. Sci. Hort. 66:59-67.
development. Physiol. Plant. 20:1059-1065.
Poignant, A. 1971. La maturation controlee de
Harris, W.V. 1927. On a lycaenid butterfly l’ananas II-L’ethrel et son action au cours des
attacking pineapple in Trinidad, British West phases ascendante et descendarte de la maturite.
Indies. Bull. Ent. Res. 18:183-188. Fruits 26:23-35.
Haruenkit, R., and A.K. Thompson. 1994. Powrie, W.D., R. Chiu, H. Wu, and B.J. Skura.
Storage of fresh pineapple. In B.R. Champ, E. 1990. Preservation of cut and segmented fresh
Highley and G.I. Johnson, eds., Proceedings fruit pieces. U.S. Patent 4,895,729.
of the International Conference on Postharvest
Handling of Tropical Fruits, pp. 422-426. Chiang Rohrbach, K.G. 1983. Exotic pineapple diseases
Mai, Thailand, July 1993. Australian Centre for and pests and their potential for spread. In K.G.
International Agricultural Research, Proceedings Singh, ed., Exotic Plant Quarantine Pests and
no. 50, Canberra, Australia. Procedures for Introduction of Plant Materials, pp.
145-171. ASEAN Plant Quarantine Centre and
Training Institute, Selangor, Malaysia.
495
Rohrbach, K.G. 1989. Unusual tropical fruit
diseases with extended latent periods. Plant Dis.
73:607-609.
496
Plum and Fresh Prune Horticultural Maturity Indices
Carlos H. Crisosto and Adel A. Kader In most of the plum cultivars grown in California,
harvest date is determined by skin color changes
Crisosto is with the Department of Plant Sciences, specific to each cultivar. A color chip guide is
University of California, Davis, CA. Kader was used to determine maturity for some cultivars.
with the Department of Plant Sciences, University Firmness, measured by squeezing fruit in the palm
of California, Davis, CA. He is deceased. of the hand (“spring”), is also a useful maturity
index for a few cultivars.
Prunes are cultivars of the European plum (Prunus Fresh prunes are picked on the basis of color:
domestica, L.) that can be dried whole. Like at least 50% of the fruit surface is red or purple
plums, prunes can be eaten fresh (if a very sweet and SSC is at least 16% in ‘Moyer’ and 19% in
fruit is desired), but they also have the high sugar ‘French’ prunes.
content necessary for successful drying. The
European plum, believed to have originated in the
Near East, has been grown in parts of Europe for Grades, Sizes, and Packaging
many centuries. Through its culture in France, the
prune ‘d’Agen’ was introduced to California from Plums and fresh prunes are hand-picked into
France by Louis Pellier, a French horticulturist bags, then dumped in bins that are moved on
who had come to California seeking gold. trailers between tree rows in the orchard. At the
packinghouse, plums are dumped (mostly using
dry bin dumps) and washed. Sorting is done to
Quality Characteristics and Criteria eliminate fruit with visual defects and sometimes
to divert fruit of high surface color to a high-
Consumer acceptance is high for fruit with quality pack. Sizing segregates fruit either by
high SSC. Fruit TA, SSC:TA, and phenolic weight or dimension. In general, plums and fresh
content (astringency) are also important factors prunes are packed into 12.6-kg (28-lb) volume-
in consumer acceptance. However, there is no filled containers.
established minimum quality standard based on
these factors. Plums with about 10 N (1 kg-force)
flesh firmness (8-mm tip) are considered ready to
eat.
497
Precooling Conditions Chilling Sensitivity
Plums and fresh prunes can be cooled in field bins Postharvest life varies among cultivars and is
using forced-air cooling, hydrocooling, or room- strongly affected by temperature management.
cooling prior to packing. Packed plums and fresh Most plum and fresh prune cultivars are
prunes should be cooled by forced-air cooling to susceptible to chilling injury when stored at 5 °C
near 0 °C (32 °F). (41 °F). Market-life of ‘Blackamber,’ ‘Fortune,’
and ‘Angeleno’ plums at 0 °C (32 °F) was >5
weeks. ‘Show Time,’ ‘Friar,’ and ‘Howard Sun’
Optimum Storage Conditions plums developed chilling injury symptoms within
4 weeks, even when stored at 0 °C (32 °F). In all
A storage temperature of -1.1 to 0 °C (30 to 32 plum cultivars, a much longer market life was
°F) with 90 to 95% RH should be used. An air achieved when stored at 0 °C (32 °F) than at 5 °C
circulation velocity of approximately 15 m3 min-1 (41 °F).
is suggested. The freezing point varies from -2 to
-1 °C (28 to 30 °F) depending on SSC.
Ethylene Production and Sensitivity
In late-season plums and in fresh ‘French’ and
‘Moyer’ prunes, internal breakdown (IB) has been Ethylene production rates are 0.01 to 5 µL kg-1 h-1
delayed by storing IB-susceptible cultivars at -1.1 at 0 °C, 0.02 to 15 µL kg-1 h-1 at 5 °C, 0.04 to 60
°C (30 °F). However, to store fruit at this low a µL kg-1 h-1 at 10 °C, and 0.1 to 200 µL kg-1 h-1 at
temperature, high SSC and excellent thermostatic 20 °C. The lower end of this range is for mature
control are essential to avoid freeze damage. but unripe fruit; higher values are for ripe fruit.
498
Physiological Disorders and storing them below 5 °C (41 °F) is effective in
controlling this fungus.
Chilling injury (CI) is a concern with most plum
and fresh prune cultivars. It is expressed as flesh
translucency associated with flesh browning. Quarantine Issues
Late plum cultivars also develop lack of juiciness
in addition to these symptoms. In previous A phytosanitary certificate is required to import
publications from South Africa, flesh translucency, California plums into Taiwan. Plums must be
specifically in some plum cultivars, has been free of Anarsia lineatella (peach twig borer),
called “gel breakdown” (Dodd 1984). In the Conotracherlus nenuphar (plum curculio), Cydia
United States, these symptoms are reported under pomonella (codling moth), Erwinia amylovora
“internal breakdown,” or CI (Kader and Mitchell (fire blight), Rhagoletis pomonella (apple
1989, Crisosto et al. 1999). CI symptoms normally maggot), Tetranychus pacificus (Pacific spider
appear after placing fruit at ripening temperatures mite), and Ceratitis capitata (Mediterranean
following cold storage at 2 to 8 °C (36 to 46 °F). fruit fly). If these conditions cannot be met, fruit
must be treated appropriately prior to shipment.
Internal browning is a physiological disorder Details of the treatment must be recorded on the
of ‘Italian’ and other cultivars of prunes. It phytosanitary certificate.
originates before harvest. It is associated with high
temperatures during fruit maturation and delayed A phytosanitary certificate is required to import
harvest. California plums into British Columbia. The
certificate should state that fruit are free of Cydia
molesta (oriental fruit moth), and it should
Postharvest Pathology clearly advertise that the fruit in the shipment
were produced and inspected in accordance
Brown rot is caused by Monilia fructicola and is with the “systems approach guidelines” agreed
the most important postharvest disease of stone to by APHIS and the Canadian Food Inspection
fruits. Infection begins during flowering. Fruit Agency. Fruit imports to all of the other Canadian
rot may occur before harvest but often develops provinces are unrestricted.
during postharvest handling. Orchard sanitation to
minimize infection sources, preharvest fungicide A similar systems approach, between APHIS and
application, and prompt precooling after harvest Mexican agencies, facilitates import and assures
are control strategies. Fruit cracking makes late plums are free of Cydia molesta (oriental fruit
season cultivars more prone to decay. Postharvest moth), Conotracherlus nenuphar (plum curculio),
fungicide treatments may be used to limit decay. Rhagoletis pomonella (apple maggot), and fruit
flies (Tephritidae).
Gray mold is caused by Botrytis cinerea. This
rot can be serious during wet spring weather.
It can develop during storage if fruit have been Suitability as Fresh-Cut Product
contaminated through harvest and handling
wounds. Avoiding mechanical injuries, effective Fresh-cut plums are best kept at 0 °C (32 °F) in
temperature management, and postharvest packages that minimize water loss. Marketing
fungicide treatments are effective control life of fresh-cut plums ranges from 2 to 5 days,
measures. depending on cultivar and stage of ripeness
(firmness) at the time of slicing.
Rhizopus rot is caused by Rhizopus stolonifer.
This rot can occur in ripe or near-ripe stone fruits
kept at 20 to 25 °C (68 to 77 °F). Precooling fruit
499
Acknowledgments Couey, H.M. 1965. Modified atmosphere storage
of ‘Nubiana’ plums. Proc. Amer. Soc. Hort. Sci.
Some of the information in this chapter is from 86:166-168.
the University of California, Davis, website on
“Produce Facts” at http://postharvest.ucdavis.edu/ Crisosto, C.H. 1994. Stone fruit maturity indices:
produce_information. a descriptive review. Postharv. News Info.
5(6):65N-68N.
Kader, A.A., and F.G. Mitchell. 1989. Postharvest Mitchell, F.G. 1987. Influence of cooling and
physiology. In J.H. LaRue and R.S. Johnson, temperature maintenance on the quality of
eds., Peaches, Plums, Nectarines: Growing and California grown stone fruit. Intl. J. Refrig. 10:77-
Handling for Fresh Market, pp. 154-164. Pub. no. 81.
3331, University of California, Davis, CA.
Mitchell, F.G., G.S. Sibbett, and G. Mayer. 1981.
Cold storage of ‘French’ prunes may expand
Further Reading dehydrator capacity. Calif. Agr. 35(1&2):20-22.
Ben, J., and M. Gaweda. 1992. Effect of Proebsting, E.L. Jr., G.H. Carter, and H.H. Mills.
increasing concentrations of CO2 in controlled 1974. Interaction of low temperature storage and
atmosphere storage on the development of maturity on quality of ‘Early Italian’ prunes. J.
physiological disorders and fungal diseases in Amer. Soc. Hort. Sci. 99:117-121.
plums (Prunus domestica L.). Folia Hort. 4:87-
100. Streif, J. 1989. Storage behaviour of plum fruits.
Acta Hort. 258:177-183.
Ceponis, M.J, R.A. Cappellini, J.M. Wells, and
G.W. Lightner. 1987. Disorders in plum, peach Truter, A.G., J.C. Combrink, and L.J. von
and nectarine shipments to the New York market, Mollendorff. 1994. Controlled-atmosphere storage
1972-1985. Plant Dis. 71:947-952. of plums. Deciduous Fruit Grower 44:373-375.
Combrink, J.C. 1996. Integrated Management of Wells, J.M., J.E. Butterfield, and M.J. Ceponis.
Postharvest Quality. INFRUITEC, Stellenbosch, 1994. Diseases, physiological disorders, and
South Africa. injuries of plums marketed in metropolitan New
York. Plant Dis. 78:642-644.
Couey, H.M. 1960. Effect of temperature and
modified atmosphere on the storage-life, ripening
behavior, and dessert quality of ‘El Dorado’
plums. Proc. Amer. Soc. Hort. Sci. 75:207-215.
500
Pomegranate Quality Characteristics and Criteria
M. Pekmezci and M. Erkan Fruit quality depends largely on sugar and acid
content of the juice. A high-quality pomegranate
Pekmezci and Erkan are with the Department should also have an attractive skin and small
of Horticulture, Faculty of Agriculture, Akdeniz seeds in the aril and should be free from sunburn,
University, Turkey. growth cracks, cuts, bruises, and decay. Skin color
and smoothness are also quality indices. Sour
and sour-sweet pomegranates have reddish skin,
Scientific Name and Introduction in contrast to sweet pomegranates which have
yellowish-green skin. Skin thickness varies from
Punica granatum L., the pomegranate, belongs 1.5 to 4.24 mm (Küpper 1995). Skin contains
to the Punicacea family and is one of the 30% tannin, which is used in medical and dye
oldest known edible fruits. It is sometimes industries.
called Chinese apple and has been cultivated
extensively in Mediterranean countries (Tunisia, Pomegranates are low in vitamin C, an important
Turkey, Israel, Egypt, Spain, and Morocco), nutritional quality component, compared to many
Iran, Afghanistan, India, and to some extent in other fruits. Vitamin C content ranges from 0.49
the United States (California), China, Japan, and to 30 mg per 100 g juice depending on cultivar
Russia. The pomegranate requires a long, hot (Hussein and Hussein 1972, Küpper 1995). Juice
summer for fruit to mature, but it can withstand content of pomegranates is 45 to 65% of the whole
low temperatures in winter and is drought- and fruit or 76 to 85% of the aril.
salt-tolerant.
501
Grades, Sizes, and Packaging
There are no U.S. grades. Only a small number of pomegranates have been grown historically in the U.S.,
mostly in California and Arizona. Therefore, most fruit are imported for the U.S. market. Pomegranates
are classified into four groups based on size. Under Turkish standards, sizes are defined as—
Fruit are generally packed into two-layer tray packs or bulk cartons.
502
Respiration Rates seeds (arils) do not develop their typical red color
and are somewhat flattened rather than plump
Pomegranate is a nonclimacteric fruit and has a (Ryall and Pentzer 1974).
very low respiration rate that declines with time in
storage.
Postharvest Pathology
-1 -1
Temperature mg CO2 kg h
5 °C 4 to 8 Gray mold rot (Botrytis cinerea), green mold rot
10 °C 8 to 16 (Penicillium digitatum), and Cladosporium spp.
20 °C 16 to 36 are the main postharvest diseases of pomegranate
Data from Crisisto et al. (1996). fruit. Gray mold usually starts at the calyx. As it
progresses, the skin becomes light-brown, tough,
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate and leathery. Heart rot is another disorder; it may
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and be caused by Aspergillus spp. and Alternaria spp.
1.8 at 20 °C (68 °F). To calculate heat production, Affected fruit show slightly abnormal skin color
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 and a mass of blackened arils; disease develops
or by 61 to get kcal tonne-1 day-1. while fruit are on the tree (Salunkhe and Desai
1984).
Physiological Disorders
Quarantine Issues
Chilling injury (CI) is the most common
physiological disorder during storage. Incidence There are no known quarantine issues.
and severity of CI depend on temperature and
duration. Symptoms are especially apparent
upon removal of fruit from cold storage to 20 °C Suitability as a Fresh-Cut Product
(68 °F). External CI symptoms include surface
pitting, skin discoloration, scald, and dead skin Possibly, but none are fresh-cut at this time.
tissues. Internal symptoms include dead tissues,
brown discoloration of the white segments
separating the arils, and pale aril color (Elyatem Special Considerations
and Kader 1984). Husk scald (brown superficial
discoloration) is another manifestation of CI in Pomegranates do not ripen after harvest and must
pomegranate; it is a restricted to the husk. At be picked fully ripe to ensure the best eating
advanced stages, scalded areas became moldy. quality.
Scald symptoms become evident after 8 weeks of
storage at 2 °C (36 °F).
References
Splitting and cracking occur in fruit on the tree.
The rind shows various degrees of cracking, which Ben-Arie, R., N. Segal, and S. Guelfat-Reich.
often serves as entry points for decay organisms 1984. The maturation and ripening of the
(Salunkhe and Desai 1984). Splitting and cracking ‘Wonderful’ pomegranate. J. Amer. Soc. Hort. Sci.
can be prevented by using regular irrigation; the 109:898-902.
last irrigation must be done 15 to 20 days before
harvest. Crisosto, C.H., E.J. Mitcham, and A.A. Kader.
1996. Pomegranates. Perishables Handling
Internal breakdown is another physiological Quarterly 85:17-18. At http://postharvest.ucdavis.
disorder in pomegranate fruit. The pulp-bearing edu/pffruits/pomegranate.
503
Elyatem, M.S., and A.A. Kader. 1984. Postharvest Mercantila. 1989. Guide to Food Transport.
physiology and storage behavior of pomegranate Fruit and Vegetables. Mercantilia, Copenhagen,
fruit. Sci. Hort. 24:287-298. Denmark.
Hardenburg, R.E., A.E. Watada, and C.Y. Morton, J. F. 1987. Fruits of Warm Climates. Julia
Wang. 1990. The Commercial Storage of Fruits, F. Morton, Miami, FL.
Vegetables, and Florist and Nursery Stocks, rev.
Agricultural Handbook 66, U.S. Department of Onur, C. 1989. Nar muhafazasında bir yenilik
Agriculture, Washington, DC. [News on pomegranate storage]. Derim 6:88-93.
Hussein, M.A.H., and M.A.S. Hussein. 1972. Onur, C., and N. Kaska. 1985. Akdeniz bölgesi
Suitability of pomegranate varieties for narlarının (Punica granatum L.) seleksiyonu
processing. Assuit J. Ag. Sci. 3:303-307. [Selection of pomegranate of Mediterranean
region]. Turkish J. Agric. For., D2, 9, 1:25-33.
Köksal, I. 1989. Research on the storage of
pomegranate cv. ‘Gökbahçe’ under different Onur, C., M. Pekmezci, H. Tibet, et al. 1992.
conditions. Acta Hort. 258:295-302. Hicaznarının soğukta muhafazası üzerinde
bir araştırma [Research on cold storage of
Küpper, W. 1995. Wirkungen von Temperatur pomegranate cv. Hicaznar]. In Proceedings of
und CO2-Konzentration in der Langfristigen CA- Turkey 1st National Horticulture Congress,
Lagerung auf Verschiedene Qualitaetsmerkmale October 13-16, 1992, Ege University Agricultural
und die Respiration Waehrend der Faculty, pp: 209-212.
Nachlagerungsphase des Granatapfels (Punica
Granatum L.) der Sorte ‘Hicaznar.’ Ph.D. Onur, C., M. Pekmezci, H. Tibet, et al. 1995.
thesis, Institut für Obstbau und Gemüsebau der Nar (Punica granatum L.) muhafazası üzerinde
Rheinschen, Friedrich-Wilhelms-Univertat., Bonn, araştırmalar [Investigations on pomegranate
Germany. storage]. In Proceedings of 2nd National
Horticulture Congreess, 3-6 October, 1995, Adana,
Küpper, W., M. Pekmezci, and J. Henze. 1995. Turkey, vol 1, pp. 696-700. Çukurova University,
Studies on CA-storage of pomegranate (Punica
granatum L., cv. ‘Hicaz’). Acta Hort. 398:101- Patil, A.V., and A.R. Karale. 1990. Pomegranate.
108. In T.K. Kose and S.K. Mitra, eds., Fruits: Tropical
and Subtropical, pp. 614-631. Naya Prokash,
LaRue, J.H. 1980. Growing pomegranates in Calcutta, India.
California. Leaflet 2459, University of California,
Division of Agricultural Science, Davis, CA. Pekmezci, M., M. Erkan, H. Gübbük, and S.
Gözlekçi. 1998. Effect of modified atmosphere on
Llacer, G., R. Martinez Valero, P. Melgarejo, et storage of pomegranate fruits (cv. Hicaznar). 25th
al. 1994. Present status and future prospects of International Horticultural Congress, Brussels,
underutilized fruit tree crops in Spain. pp. 63-75. Belgium. Acta Hort. PP2/04/A10:368
In First Meeting CIHEAM Coop. Res. Network
Underutilized Fruit Trees, 1994, Zaragoza, Spain. Ryall, A.L., and W.T. Pentzer. 1974. Handling,
Transportation and Storage of Fruit and
Mars, M. 1994. La culture du granadier (Punica Vegetables, vol. 2. AVI, Westport, CT.
granatum L.) et du figuier (Ficus carica L.) en
tunisie. pp. 76-83. In First Meeting CIHEAM Salunkhe, D.K., and B.B. Desai. 1984. Postharvest
Coop. Res. Network Underutilized Fruit Trees, Biotechnology of Fruits, vol. 2. CRC Press, Boca
1994, Zaragoza, Spain. Raton FL.
504
SeaLand Service. 1991. Shipping guide to
perishables. SeaLand Service, Iselim, NJ.
Further Reading
505
Potato overmaturity resulting in higher sugar levels. Vine
senescence is used as a preharvest maturity index,
Ronald E. Voss but the correlation between vine senescence and
tuber skin-set varies among cultivars.
Voss is with the Department of Plant Sciences,
University of California, Davis, CA.
Grades, Sizes, and Packaging
Scientific Name and Introduction USDA market grades (established in 1991) are
Extra No. 1, No. 1, Commercial, and No. 2; grade
The white, or Irish, potato, Solanum tuberosum L., is distinguished primarily on external condition
is an annual of the Solanaceae family. The edible and appearance. Grades vary among tuber shapes.
portion is the tuber, an underground stem that The minimum diameter for U.S. No. 1 or No. 2
forms at the end of stolons. Potato is a cool-season is 4.8 to 5.1 cm (1 7/8 to 2 in) for round potatoes
plant. It is native to the Americas, with many close with a weight minimum of 118 g (4 oz) for long
relatives in the Andean region of South America. potatoes. At times, an additional requirement that
New varieties commonly have one or more wild ≥60% of the tubers must be a minimum of 148 g
Solanum species included in their pedigree. Potato (5 oz) is imposed. A “B-size” grade is becoming
is grown throughout the world in temperate zones, more common, primarily for round red and white
with planting in spring and harvest in fall. potatoes. B-size grade tubers are <5.1 cm (2 in).
There are many skin colors (brown russet, white, Oblong and long russets are commonly wholesale
red, pink, yellow) and flesh colors (white, cream, marketed in “count” boxes of 60, 70, 80, 90 or 100
yellow, blue/purple/red, and striated). Tuber tubers in a 22.5-kg (50-lb) carton, thus averaging
shapes vary from round to oblate to oblong to approximately 380, 325, 296, 266, or 237 g each
long. Stored potatoes are available year-round; (13, 11, 10, 9, or 8 oz), respectively. These are
fresh potatoes are harvested year-round, albeit retailed bulk for consumers to select individual
in small quantities during winter, spring, and tubers. Common retail packages of 2.27 and 4.55
summer. Processed potatoes (frozen french fries, kg (5 and 10 lbs) plastic and paper bags generally
potato chips, flakes, etc.) are manufactured year- contain 150- to 240-g (5- to 8-oz) tubers. B-size
round from fresh and stored tubers. tubers are sold in pint “strawberry” baskets or in
bulk. Prepeeled tubers are generally packaged in
plastic trays covered with plastic wrap.
Quality Characteristics and Criteria
Potatoes for processing into such products as
A high-quality fresh-market potato tuber is french fries or chips are graded using the U.S.
turgid, well shaped, uniform, and brightly colored grade and size standards in combination with
(especially reds, whites, and yellows), as well individual quality and size requirements of each
as free from adhering soil, mechanical damage, processing company. The company requirements
greening, sprouts, diseases, and physiological vary with the type of product to be manufactured
defects. and the purchaser. Tubers are delivered to
processing plants in bulk trucks or bins holding
about 2,000 lb (910 kg).
Horticultural Maturity Indices
The ability of potato tuber skin to resist abrasion Optimum Storage Conditions
(skinning) during harvest is a common index for
maturity. Sugar content is a maturity index for Long-term storage of potato tubers—up to
processing potatoes, with both immaturity and 12 mo—requires that they be cured. Curing
506
stimulates suberization and wound healing and facilities, variety, and whether or not sprout
reduces respiration. Optimal curing conditions inhibitors are used. Sprout inhibitor may be
are approximately 20 °C (68 °F) with RH of 80 to applied in the field before senescence begins, on
100%. Curing is markedly slower at temperatures the tubers as they are graded and packaged, or in
<12 °C (54 °F) and >25 °C (77 °F). Similarly, storage after curing is completed.
RH <80% delays curing. Curing at 15 °C (59 °F)
is commonly recommended to minimize decay.
Frequently, potatoes harvested in fall in temperate Controlled Atmosphere (CA) Conditions
climates are colder than the optimal curing
temperatures. However, respiration in storage The usefulness of CA storage is minimal, and
will heat the tubers and raise RH. Temperature economic justification doubtful. Periderm
and RH control during this “sweating” or curing development and wound healing are delayed at
time is managed using fans to bring outside air atmospheres with <5% O2. Injury from <1.5%
into the storage room, depending on the need O2 or >10% CO2 includes induced off odors and
during the night or day. Once cured, a process flavors, internal discoloration, and increased
requiring 1 to 2 weeks, the tuber temperature is decay. While higher CO2 levels tend to inhibit
lowered by 1 to 2 °C per day until the desired formation of reducing sugars, they also increase
maintenance temperature and RH are reached. sucrose content.
In some cases, two additional steps must be
employed: drying of wet potatoes upon entry into
storage, and heating of tubers before removal Retail Outlet Display Considerations
from storage. Forced movement of air is used to
ensure uniform temperature throughout the storage Cured and new potatoes, whether displayed in
pile. Maintaining 95 to 99% RH is required at all bulk or in cellophane or paper bags are displayed
times to minimize shrink and pressure bruising in dry. New potatoes that are displayed in bulk
storage. are commonly included with other cool-season
vegetables that receive periodic water misting or
Desired maintenance temperature depends on the sprinkling.
desired end use of the tubers. Respiration rate
of potato tubers is lowest at 2 to 3 °C (36 to 37
°F). Storage at 0 to 2 °C (32 to 36 °F) increases Chilling Sensitivity
the risk of freezing or chilling injury. Sprouting
accelerates at temperatures >4 °C (40 °F), so seed Potatoes freeze at approximately -1 °C (30 °F).
tubers are commonly stored at 4 to 5 °C (40 °F). Internal mahogany browning can occur at 1 to
Tubers for fresh consumption are stored at 7 to 2 °C (34 to 36 °F), while temperatures of 3 to
10 °C (45 to 50 °F), to minimize conversion of 4 °C (37 to 39 °F) typically result in increased
non-reducing sugars such as starch to reducing reducing sugar levels that are not reversible with
sugars such as glucose, which darken during reconditioning.
cooking. Tubers for frying are stored at 10 to 15
°C (50 to 59 °F), depending on the cultivar and its
respective sugar conversion characteristics. Many Ethylene Production and Sensitivity
chipping cultivars accumulate excessive sugar if
stored <15 °C (59 °F). Thus, chipping cultivars are Potatoes produce very low levels of ethylene:
stored at 15 to 20 °C (59 to 68 °F); new cultivars <0.1 µL kg-1 h-1 at 20 °C (68 °F). Cut, bruised,
are being developed that will not accumulate sugar or wounded tubers have greatly increased
at temperatures as low as 5 to 10 °C (41 to 50 °F). ethylene production rates. Potato tubers are not
very sensitive to external ethylene. Low levels
Quality tubers can be stored for 2 to 12 mo, of ethylene elevate respiration, especially in
depending on quality at harvest, quality of storage immature potatoes, and result in weight loss and
507
mild shriveling. After 2 to 3 mo at >5 °C (41 °F) Blackheart is a storage- or transportation-induced
without sprout inhibitor, low levels of ethylene disorder caused by low O2. Typically, blackheart
may retard sprouting, while high amounts may is induced at >30 °C (86 °F), which increases
induce sprouting. respiration. If air exchange around tubers is
insufficient, low-O2 conditions develop in the
interior of the tuber, and the cells suffocate
Respiration Rates and turn black. Blackheart is rare in early crop
potatoes because of typical marketing practices.
Immature potato tubers usually have higher
respiration rates than mature or cured tubers. Freezing at -1 °C (30 °F), whether in the field or in
Cooler temperatures and increased air movement storage, typically results in a distinct demarcation
are effective at controlling effects of a high rate of between affected and unaffected tissue. Symptoms
respiration. Increased air movement in the absence include a water-soaked appearance, glassiness,
of high RH, however, will cause desiccation. and tissue breakdown on thawing. Chilling injury
can occur after a few weeks at 0 °C (32 °F) and
Temperature Immature Mature (cured) result in a mahogany discoloration of internal
--------mg CO2 kg-1 h-1--------- tissue in some varieties. Much longer periods of
5 °C 24 6 to 18 storage are generally required for chilling injury.
10 °C 30 to 40 13 to 19
15 °C 25 to 57 11 to 22 Greening may occur in part of a tuber exposed
20 °C 32 to 81 14 to 29 to light. Affected tubers are easily culled at
grading and rarely proceed to marketing channels.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Darkness is essential for long-term storage
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and because greening can occur during storage
1.8 at 20 °C (68 °F). To calculate heat production, or marketing. Exposure to bright light during
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 postharvest handling, or longer periods (1 to 2
or by 61 to get kcal tonne-1 day-1. weeks) of low light, can result in development
of chlorophyll (greening) and bitter, toxic
glycoalkaloids such as solanine. Solanine also
Physiological Disorders forms in response to bruising and wounding
(including fresh processing followed by storage)
The most common and serious physiological and during sprouting. Glycoalkaloids are heat
disorders affecting potatoes include black spot, stable and minimally degraded by cooking. Tubers
blackheart, freezing injury, greening, hollow heart, in market displays should be replaced daily or
sugar end browning, and internal necrosis. more frequently to minimize greening.
Black spot results from a physical impact to the Enlarged lenticels are a common disorder in
tuber; the stem end is most sensitive. Following early potatoes where excessive irrigation is often
severe bruising or cutting, the affected tissue turns applied to maintain cooler soil temperatures in
reddish, then blue, becoming black in 24 to 72 warm or hot climates. These lenticels are subject
h. Severity increases with time. Cultivars differ to pathological infection in the soil or during
significantly in their susceptibility and symptom packing. Infections may remain innocuous; or
expression. Soil conditions can predispose tubers if transportation conditions are not properly
to blackspot; poor aeration is the most common maintained, they can increase rapidly in severity.
cause. Proper fertilization (particularly potassium), Tubers that appear sound at the packing shed can
water management, careful handling, and high RH become unmarketable during transit.
to maintain turgidity are important in minimizing
black spot. Use of compost and manure helps
prevent blackspot.
508
Skinning is a common disorder in early crop or Quarantine Issues
“new” potatoes (harvested immature). Soil drying
and vine death enhance skin set, and thus decrease Export and import of potato tubers can involve
skinning. Cultivars vary in ability to set-skin, in numerous quarantine issues related to grade,
skin thickness, and thus in skinning susceptibility. diseases, and nematodes. Each country has its
“New” potatoes must be kept at a very high RH, own phytosanitary requirements. Inspections
near 100%, and must be handled with special care. and appropriate authorizations are required.
Among the most common diseases and nematodes
Hollow heart, sugar end accumulation, and included in quarantine or zero-tolerance
internal necrosis are all production problems requirements are cyst nematode (Globodera spp.),
related to irregular growth, inadequate water viruses and viroids, brown rot (Pseudomonas
availability, or widely fluctuating temperatures. solanacearum), ring rot (Corynebacterium
These conditions do not change during harvest and sepedonicum), and powdery scab (Spongospora
postharvest handling. subterranean). Similarly, these diseases and
nematodes are restricted on potato tubers to be
imported. Currently, potato tubers may not be
Postharvest Pathology imported into the United States from any country
except Canada.
Diseases are an important source of postharvest
loss, particularly in combination with rough
handling and poor temperature control. Three Suitability as Fresh-Cut Product
major bacterial diseases and a greater number of
fungal pathogens are responsible for occasional, Potatoes are relatively new as a lightly processed
serious postharvest losses. The major bacterial product. Fresh-cut potatoes are not marketed,
and fungal pathogens that cause postharvest but par-boiled whole tubers can be prepared and
losses in transit, in storage, and after-market are marketed in plastic trays with sealed plastic wrap
bacterial soft rot (Erwinia carotovora subsp. with low O2-transmission characteristics. Storage
carotovora and subsp. atroseptica), Ralstonia (ex requirements for tubers to be processed in this
Pseudomonas, ex Burkholderi) solanacearum, manner have not been well defined.
late blight (Phytophthora infestans), fusarium
dry rot (Fusarium spp.), pink rot (Phytophthora
spp.), water rot (Pythium spp.), and silver scurf Special Considerations
(Helminthosporium solani). Occasionally serious
diseases of immature tubers include pink eye Potatoes may impart an “earthy” odor to apples
(Pseudomonas fluorescens) and grey mold and pears if held in storage with low air exchange.
(Botrytis cinerea). Potatoes may acquire an off flavor from odor
volatiles released by other produce items.
In addition to careful sorting before placing
tubers into storage, management of air, RH, Postharvest handling and storage of late-crop
and temperature during storage and transit of potatoes is very complex and depends on growing
potatoes with potential problems can be effective. conditions, environment at harvest, variety,
Lower RH, shortened curing time, and lower intended use, and many other factors.
temperatures can minimize spread of rot diseases.
509
Further Reading Strand, L., G. Bishop, G. Kleinschmidt, et al., eds.
1992. Integrated pest management for potatoes in
Kader, A.A., ed. 2002. Postharvest Technology of the western United States, rev. DANR Pub. no.
Horticultural Crops, 3rd ed. DANR Pub. no. 3311, 3316 and Western Regional Res. Pub. no. 011,
University of California, Oakland, CA. University of California, Oakland, CA.
Kleinkopf, G.E. 1979. Translucent end of Suslow, T., and R. Voss. 2000. Potato (immature
potatoes. Cur. Info. Ser. 488, University of Idaho, early crop). Recommendations in maintaining
Moscow, ID. postharvest quality. Postharvest Technology
Research & Information Center, University of
Kleinkopf, G.E. 1995. Dynamics of the stored California, Davis, CA.
potato—a management approach. Early season
storage. Amer. Potato J. 72:449-462.
510
Prickly Pear Grades, Sizes, and Packaging
Quality Characteristics and Criteria Depending on variety, ripeness stage, and harvest
season, fruit can be kept for 2 to 5 weeks at 5 to
Fruit of high quality need to be harvested near 8 °C (41 to 46 °F) with 90 to 95% RH. Factors
full ripeness to have color and flavor typical of limiting storage life are decay, dehydration, and
each variety. They should have a high percentage chilling injury.
of pulp, low seed content, and peel that is easy to
remove. Fruit typically have high sugar content
(12 to 17% SSC) and low acidity (0.03 to 0.12% Controlled Atmosphere (CA)
TA). Cactus pears contain considerable amounts Considerations
of vitamin C (200 to 400 µg g-1). If harvested at
maturity from good varieties, fruit have a delicate Limited research indicates that holding cactus pear
sweet flavor that differs by variety. at 5 °C (41 °F) in 2% O2 and 2 to 5% CO2 delays
ripening and extends storage life (Kader 2000).
511
Chilling Sensitivity to 131 °F) for 5 min and fungicide-containing
waxes may reduce surface decay, but are not
Cactus pear fruit are especially chilling sensitive effective when there is damage to the stem-ends.
when stored at <5 °C (41 °F), but chilling injury For control of decay on stem-ends, see Special
may occur in some varieties at <10 °C (50 °F). Considerations. Preharvest calcium sprays result
Symptoms include pitting, surface bronzing and in less postharvest decay (Schirra et al. 1999).
dark spots on the peel, and increased susceptibility
to decay. Chilling occurred in a red-fruit variety
after only 2 weeks at 6 °C (43 °F), but fruit Quarantine Issues
from other varieties were held for several weeks
without signs of chilling. Summer-harvested fruit There are no known quarantine issues.
are more chilling sensitive than autumn-harvested
fruit (Schirra et al. 1999). Application of calcium
chloride, conditioning, and intermittent warming Suitability as Fresh-Cut Product
have had variable success in reducing chilling
injury. Even after brushing and washing, the peel of intact
cactus fruit may have irritating small spines, and
a peeled packaged fruit product could be a useful
Ethylene Production and Sensitivity option. A mixture of cactus fruit of different colors
and types could be a potentially attractive product.
Prickly pear fruit produce very low amounts of
ethylene, about 0.2 µL kg-1 h-1 at 20 °C (68 °F).
They are not sensitive to ethylene exposure. Special Considerations
512
Schirra, M., P. Inglese, and T. La Mantia. 1999.
Quality of cactus pear [Opuntia ficus-indica (L.)
Mill.] fruit in relation to ripening time, CaCl2
preharvest sprays and storage conditions. Sci.
Hort. 81:425-436.
Further Reading
513
Pumpkin and Winter Squash Horticultural Maturity Indices
Pumpkins and winter squash should be fully Pumpkins and winter squash should be room-
mature, with hard rinds and, except for some cooled or loaded directly into refrigerated trucks
striped varieties, solid external color. Good-quality and containers.
pumpkins and winter squash have bright-yellow
or orange flesh with fine, moist texture and high
solids, sugars, and starch (Cantwell and Suslow Optimum Storage Conditions
1998). Overmature flesh can become dry and
stringy. All pumpkins and winter squashes should be
well matured, carefully handled, and free from
injury or decay. Pumpkins and winter squashes
are placed on racks, in bulk bins, or in baskets and
514
are often held in ventilated or common storage in Retail Outlet Display Considerations
production areas. Recommended conditions for
storage of pumpkins and winter squashes are 10 to Pumpkins and winter squash can be displayed in
13 °C (50 to 55 °F). At higher temperatures of 15 ambient conditions.
to 20 °C (59 to 68 °F), green varieties will become
undesirably yellow and acquire a stringiness of
the flesh. Fruit are chilling sensitive (see Chilling Chilling Sensitivity
Sensitivity). The RH should be 50 to 70%. Higher
RH promotes decay while lower RH causes excess Both pumpkins and winter squashes develop
weight loss and texture deterioration (Ryall and chilling injury at <10 °C (50 °F). Storage at
Lipton 1979). The fruit surface should be kept 0 to 4 °C (32 to 39 °F) inhibits yellowing but
dry, and storage rooms should have good air causes sunken pits on the fruit surface and loss
circulation (Holmes 1951). of flavor. Alternaria rot develops on chilled
squashes after removal from storage (McColloch
Pumpkins generally do not keep as well as hard- 1962). Depending on the cultivar, chilling injury
shelled winter squashes. Most cultivars of winter symptoms develop after 1 mo at 5 °C (41 °F) or
squash and pumpkins, as well as the tropical several months at 10 °C (50 °F) (Cantwell and
pumpkins, cannot be stored for more than 2 Suslow 1998).
to 3 mo. Acorn-type squashes, such as ‘Table
Queen,’ can be kept 5 to 8 weeks at 10 °C (50
°F). The popular butternut squash can be kept 2 Ethylene Production and Sensitivity
to 3 mo at 10 °C (50 °F). It is often stored longer,
but spoilage and shrinkage increase. Weight Pumpkins and winter squash produce only trace
loss should be kept below 15% to minimize amounts of ethylene, but wounding greatly
development of hollow neck (Francis and increases ethylene production (Hyodo et al. 1993).
Thomson 1965). Turban and buttercup squashes Hubbard squash and other dark-green-skinned
can be kept 3 mo. Good quality Hubbard squash squashes should not be stored near apples, as the
can be stored 6 mo at 10 to 13 °C (50 to 55 °F) ethylene from apples may cause the skin to turn
and 70% RH. A 15% loss in weight after 6 mo is orange-yellow (Yeager et al. 1945). Ethylene
about average (Guba 1950). may also cause stem abscission, especially in less
mature fruit (Cantwell and Suslow 1998).
515
Physiological Disorders Special Considerations
Damaged areas on fruit turn brown, detracting A 10- to 20-day curing period at 24 to 27 °C (75
from their appearance. Pumpkins and winter to 81 °F) before storage can harden the rind of
squash are susceptible to water loss at the pumpkins and winter squashes (Gorini and Testoni
recommended 50 to 70% RH, but low RH is 1978). However, in New York, curing for 3 weeks
necessary to minimize decay. Yellowing can be at 27 °C (81 °F) to heal mechanical injuries and
a problem for green winter squash varieties and to ripen immature specimens proved unnecessary
is exacerbated by overmaturity, high storage (Platenius et al. 1934, Schales and Isenberg 1963).
temperatures, and ethylene exposure. Curing butternut, Hubbard, and quality squashes
was of no value but not harmful, whereas curing
‘Table Queen’ was detrimental to skin color,
Postharvest Pathology texture, and taste (Schales and Isenberg 1963).
Cured ‘Table Queen’ also decayed more rapidly
Decay is the primary cause of storage losses than noncured fruit.
of pumpkins and winter squash. Numerous
fungi cause storage rots, including species of
Aspergillus, Colletotrichum (anthracnose), References
Didymella, Fusarium, Mycosphaerella (black
rot), Rhizopus, and Sclerotinia (Guba 1950, Abdel-Rahim, A.M. 1988. Postharvest fungal
Abdel-Rahim 1988, Hawthorne 1988, Rath diseases of some vegetables in Kuwait. Arab J.
et al. 1990, Vigliola 1993, Arvayo-Ortiz et al. Plant Protect. 6:83-87.
1994). Alternaria rot develops on chill-damaged
fruit (McColloch 1962). Delaying harvest of Arvayo-Ortiz, R.M., S. Garza-Ortega, and E.M.
buttercup squash 2 weeks increased fungal Yahia. 1994. Postharvest response of winter
storage rots (Hawthorne 1990). Infection starts squash to hot-water treatment, temperature and
through wounds and natural openings in the length of storage. HortTechnology 4:253-255.
surface. Therefore, careful handling to minimize
mechanical damage is recommended to minimize Cantwell, M., and T.V. Suslow. 1998. Pumpkins
storage rots (Guba 1950). Less rot will develop in and winter squashes. Recommendations for
Hubbard squash if stems are completely removed maintaining postharvest quality. Perishables
before storage (Yeager et al. 1945). Hot water Handling Quarterly 94:15-16.
at 60 °C (140 °F) for 2 min reduces storage rots
(Francis and Thomson 1965); lower temperatures Daniel, A.L. 1995. Tropical Pumpkin Cultigen
were not effective (Hawthorne 1989, Arvayo-Ortiz Postharvest Quality Evaluation and Maturity
et al. 1994). Studies. M.Sc. thesis, University of Florida,
Gainesville, FL.
Quarantine Issues Daniel, A.L., J.K. Brecht, C.A. Sims, and D.N.
Maynard. 1995. Sensory analysis of bush and
There are no known quarantine issues. vining types of tropical pumpkin. Proc. Fla. State
Hort. Soc. 108:312-316.
Suitability as Fresh-Cut Product Edelstein, M., H. Nerson, and H.S. Paris. 1989.
Quality of spaghetti squash as affected by fruit
Some large winter squash are cut into sections and maturity, storage period, and cooking duration.
seeds removed for retail sale. Acta Hort. 258:543-545.
516
Francis, F.J., and C.L. Thomson. 1965. Optimum Lin, W.C., and M.E. Saltveit. 1997. Quality of
storage conditions for butternut squash. Proc. winter squash affected by storage air composition
Amer. Soc. Hort. Sci. 86:451-456. and temperature. In M.E. Saltveit, ed., 7th
International Controlled Atmosphere Research
Gorini, F.L., and A. Testoni. 1978. Long-term Conference, July 13-18, 1997, University of
storage of pumpkins. Ann. Ist. Sper. Valoriz. California, Davis, vol. 4, pp. 78-83. University of
Technol. Prod. Agr. 9:131-135. California, Davis, CA.
Guba, E.F. 1950. Spoilage of squash in storage. McColloch, L.P. 1962. Alternaria rot following
Massachusetts Agricultural Experiment Station chilling of acorn squashes. Marketing Research
Bull. no. 457, University of Massachusetts, Report 518, U.S. Department of Agriculture,
Amherst, MA. Washington, DC.
Harvey, W.J., D.G. Grant, and J.P. Lammerink. Platenius, H., F.S. Jamison, and H.C. Thompson.
1997. Physical and sensory changes during 1934. Studies on cold storage of vegetables. New
development and storage of buttercup squash. York (Cornell) Agricultural Experiment Station,
N.Z. J. Crop Hort. Sci. 25:341-351. Bull. no. 602, Cornell University, Ithaca, NY.
Hawthorne, B.T. 1988. Fungi causing storage Prange, R.K., and P.A. Harrison. 1993. Effect
rots on fruit of Cucurbita spp. N.Z. J. Exp. Agr. of controlled atmosphere and humidity on
16:151-157. postharvest physiology of buttercup winter squash,
Cucurbita maxima Duch. hybrid ‘Sweet Mama.’
Hawthorne, B.T. 1989. Effects of cultural In 6th International Controlled Atmosphere
practices on the incidence of storage rots in Research Conference, June 1993, vol. 2, pp. 759-
Cucurbita spp. N.Z. J. Crop Hort. Sci. 17:49-54. 766. Ithaca, NY.
Hawthorne, B.T. 1990. Age of fruit at harvest Rath, G.C., D. Mishra, and N.C. Nayak. 1990.
influences incidence of fungal storage rots on fruit A note on fungi causing rotting of cucurbits in
of Cucurbita maxima D. hybrid ‘Delica.’ N.Z. J. Orissa markets. Orissa J. Agr. Res. 3:161-162.
Crop Hort. Sci. 18:141-145.
Ryall, A.L., and W.J. Lipton. 1979. Handling,
Holmes, A.D. 1951. Factors that affect the storage Transportation, and Storage of Fruits and
life of butternut squashes. Food Technol. 5(9):372- Vegetables, 2nd ed., vol. 1. AVI, Westport, CT.
373.
Schales, F.D., and F.M. Isenberg. 1963. The effect
Hyodo, H., C. Hashimoto, S. Morozumi, et al. of curing and storage on chemical composition
1993. Induction of wound ethylene production and and taste acceptability of winter squash. Proc.
lignin formation in wounded mesocarp tissue of Amer. Soc. Hort. Sci. 83:667-674.
Cucurbita maxima. Acta Hort. 343:264-269.
Vigliola, I. 1993. Postharvest diseases and
Irving, D.E., P.L. Hurst, and J.S. Ragg. 1997. storage conditions in butternut squash (Cucurbita
Changes in carbohydrates and carbohydrate moschata Duch.) in Argentina. Acta Hort. 368:33-
metabolizing enzymes during development, 36.
maturation, and ripening of buttercup squash
(Cucurbita maxima D. ‘Delica’). J. Amer. Soc. Yeager, A.F. 1945. The Storage of Hubbard
Hort. Sci. 122:310-314. Squash. New Hampshire Agricultural Experiment
Station Bulletin, The University of New
Hampshire Bull. no. 356, Durham, NH.
517
Quince Horticultural Maturity Indices
Though the skin of quince has a thick coat of fuzz, Iocheva, E. 1979. Change of catechins in pome
the fruit is tender and bruises easily (Auchter and fruit during technological processing [quince].
Knapp 1929). Quince is subject to decays similar Khranitelna promishlenost 28:33-35.
to those found on apples and pears (Halsted, 1892,
Tafradzhiiski and Angelov 1977). Treatment with Jankowiak, J. 1976. Quince is a “new” old-
fungicides is necessary to prevent decay during fashioned fruit. Org. Gard. Farming 23(8):114-
storage and marketing (Pierson et at. 1971). 115.
Durmishidze, S.V., and N.P. Gumbaridze. 1972. Lutz, A., and P. Winterhalter. 1992a. Bio-oxidative
Catechins and leucoanthocyanidins of quince fruit. cleavage of carotenoids: important route to
Soobshch. Akad. Nauk. Gruz. Ssr. 67:449-451. physiological active plant constituents. Tetra. Lett.
33:5169-5172.
Guldner, A., and P. Winterhalter. 1991. Structures
of two new ionone glycosides from quince fruit Lutz, A., and P. Winterhalter. 1992b. Isolation of
(Cydonia oblonga Mill.). J. Agric. Food Chem. additional carotenoid metabolites from quince
39:2142-2146. fruit (Cydonia oblonga Mill.). J. Agric. Food
Chem. 40:1116-1120.
519
Lutz, A., and P. Winterhalter. 1993. Abscisic Tsuneya, T., M. Ishihara, H. Shiota, and M.
alcohol glucoside in quince. Phytochem. 32:57-60. Shiga. 1983. Volatile components of quince fruit
(Cydonia oblonga Mill.). Agric. Biol. Chem.
Michelesi, J.C., J. Brossier, and J.D. Flick. 1973. 47:2495-2502.
Premieres observations sur plusieurs varietes de
cognassiers a fruits [First observations on several Umano, K., A. Shoji, Y. Hagi, and T. Shibamoto.
quince fruit varieties]. Arboric. Fruit 233/234:36- 1986. Volatile constituents of peel of quince fruit,
38. Cydonia oblonga Miller. J. Agric. Food Chem.
34:593-596.
Muller, W., and A. Husistein. 1979. Quitte—eine
zu beachtende obstart [Quince—a fruit species of Westwood, M.N. 1978. Fruit and nut species. In
interest]. Schweizerische Zeitschrift for Obst- und M.N. Westwood, ed., Temperate-Zone Pomology,
Weinbau 115(1):4-8. pp. 41-76. W.H. Freeman, San Francisco, CA.
Pierson, C.F., M.J. Ceponis, and L.P. McColloch. Williams, W.J. 1935. Cold storage of figs, raisins,
1971. Market Diseases of Apples, Pears and persimmons, quinces, and tobacco. Ice Refrig.
Quinces. Agriculture Handbook 376, U.S. 89:367-368.
Department of Agriculture, Washington, DC.
520
Radicchio Horticultural Maturity Indices
Radicchio (Cichorium intybus L.) is a member There are no USDA grades. Head size is based
of the Asteraceae (Compositae) family. Plants on weight: 30 g (1 oz) for forced radicchio and
are characterized by variegate leaves (var. 60 g (2 oz) for normal production. Extra fancy
silvestre Bishoff) or red leaves (consequence of radicchio has the quality criteria given above.
spontaneous crossings). Plant shape also differs Fancy radicchio can have some light browning
among varieties, including long conical shape on leaves due to freezing or forcing treatment.
with tight red leaves, completely open with red or Radicchio can be packed in trays with shrinkable
green-red leaves, or ball-shaped with red leaves. plastic film or in corrugated containers with plastic
Head and leaves are crisp with a bittersweet liners to avoid water loss (Suslow and Cantwell
flavor similar to Belgian endive and escarole. 1999).
Some varieties—for example ‘di Treviso’ and
‘Verona’—are held in the dark for 7 to 15 days at
10 to 15 °C (50 to 68 °F) after harvest to promote Precooling Conditions
production of new, very tender leaves; this is
called “whitening” (Pimpini 1990). Radicchio is Radicchio is usually placed directly in a cold room
available year-round from California, New Jersey, before shipping.
Mexico, and Italy.
Optimum Storage Conditions
Quality Characteristics and Criteria
Radicchio can be stored for 20 to 30 days at 3 to 5
Plants and leaves should be very turgid and crisp °C (37 to 41 °F) with 90% RH. Varieties that are
with bright white midribs. There should be no whitened can only be stored 1 to 2 weeks under
cracking, splitting, dark spots from mechanical these conditions. Storage life is reduced to 11 days
damage, or necrosis on the leaf margins. at 6 °C (43 °F) (Botondi et al. 1996), while storage
Compactness is important but depends on the at -2 to -3 °C (27 to 28 °F) can prolong storage to
variety; for example, ‘Treviso’ and ‘Chioggia’ 3 mo by controlling decay (Bertolini and Pratella
should have a compact head and very tight leaves, 1993).
whereas ‘Verona’ and ‘Castelfranco’ should have
an open, regularly shaped head. The root must be
cleanly cut with no secondary roots or evidence of Controlled Atmosphere (CA)
decay and cannot exceed 4 cm (1.5 in). Considerations
Radicchio produces very low amounts of ethylene: There are no known quarantine issues.
0.1 to 0.3 µL kg-1 h-1 at 0 to 6 °C (32 to 43 °F) and
0.6 to 1.0 µL kg-1 h-1 at 20 °C (68 °F). Exposure
to 10 µL L-1 ethylene for 6 days at 7.5 °C (46 °F) Suitability as Fresh-Cut Product
with 95% RH increased leaf browning, decay,
and pigmentation of midribs (Botondi et al. 1996, There is growing interest in fresh-cut lettuce-
Suslow and Cantwell 1999). radicchio mixes.
Temperature mg CO2 kg-1 h-1 Bertolini, P., and G. Pratella. 1993. Protezione
0 °C 6 to 10 postraccolta del radicchio. I Radicchi Veneti: 20
6 °C 10 to 16 Anni di Esperienze Produttive e Commerciali, p.
7.5 °C 23 39. ICE, Verona, Italy.
25 °C 45
Botondi, R., R. Massantini, G. Anelli, and F.
-1 -1 -1
To get mL CO2 kg h , divide the mg kg h rate -1 Mencarelli. 1996. Postharvest physiology of red
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and cicory stored at 0° and 6°C. Ital. J. Food Sci.
1.8 at 20 °C (68 °F). To calculate heat production, 4:273-282.
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1. Foschi F., and M. Mari. 1987. Controlled
atmosphere storage techniques of red chicory. In
Proc. 17th International Congress on Refrigeration,
Physiological Disorders vol. C, pp. 290-295. Vienna, Austria. International
Institute of Refrigeration, Paris, France.
Desiccation can lead to necrosis of the leaf
margins. Wilting can occur after a few days of Pimpini, N. 1990. Il radicchio. In V.V. Bianco and
display if plants are not misted. Leaf abscission is F. Pimpini, eds., Orticoltura, pp. 320-338. Patron
common for some varieties, and reconditioning is Press, Bologna, Italy.
often required. Rough handling during harvest or
522
Suslow, T., and M. Cantwell. 1999. Radicchio.
Recommendations for maintaining postharvest
quality. At http://postharvest.ucdavis.edu/produce_
information.
523
Radish between 2 and 3 cm (0.75 to 1.25 in). Bunched
radishes are harvested in either regular or big
Richard LaMar Hassell bunch size, and roots for cello packages are pulled
at regular or jumbo size (Nonnecke 1989).
Hassell is with the Coastal Research and
Education Center, Clemson University,
Charleston, SC. Grades, Sizes, and Packaging
524
the specific amount of desired radishes, and seal Chilling Sensitivity
the bag or provide a zipper locking system. These
bags are then hand-packed into wax cartons and Radishes are not sensitive to chilling. Store them
temporarily stored at 2 °C (36 °F) until shipment. as cold as possible without freezing.
Hydrocooling at 0 to 4.5 °C (32 to 40 °F) is the Radishes produce small amounts of ethylene and
preferred method. Cello packs are hydrocooled are not particularly sensitive to ethylene exposure.
to restore crispness, bulk stored in bins, and then
placed in refrigerated coolers before grading.
Bunched radishes are dipped in chlorinated water Respiration Rates
at 2 °C (26 °F) to restore crispness and freshness
to the tops and roots, as well as to remove field Temperature Topped Roots Bunched Roots
debris. They are then packed into cartons, usually with Tops
24 bunches per carton, and hydrocooled; and the °C -----------mg CO2 kg-1 h-1-----------
carton is topped with an ice slurry before shipping 0 14 to 17 3 to 9
(Brooker and Pearson 1970, Ryall and Lipton 4 to 5 19 to 21 6 to 13
1972a). 10 31 to 36 15 to 16
15 to 16 70 to 78 22 to 42
20 to 21 124 to 136 44 to 58
Optimum Storage Conditions 25 to 27 158 to 193 60 to 89
Data from Hardenburg et al. (1986).
Topped radishes can be held 3 to 4 weeks at 0 °C
(32 °F) with 90 to 95% RH and at least 7 days at To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
7 °C (45 °F). Bunch radishes are harder to keep by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
fresh due to the perishability of the tops. However, 1.8 at 20 °C (68 °F). To calculate heat production,
they can be held at 0 °C (32 °F) and 90 to 95% multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
RH for 1 to 2 weeks. Winter or black radishes can or by 61 to get kcal tonne-1 day-1.
be stored under the same conditions for 2 to 4 mo.
Addition of top ice aids in keeping the tops fresh
(Ryall and Lipton 1972b). Physiological Disorders
525
Postharvest Pathology Carcione, J., and B. Lucas. 1972. The Greengrocer:
The Consumer’s Guide to Fruits and Vegetables.
The initial lesions of bacterial black spot Chronicle Books, San Francisco, CA.
(Xanthomonas vesicatoria) are brown and have a
diameter of 1.0 to 2.5 mm (0.04 to 0.1 in). They Hardenburg, R.E., A.E. Watada, and C.Y.
eventually turn black and coalesce. Protective Wang. 1986. The Commercial Storage of Fruits,
measures include washing in water with 100 Vegetables, and Florist and Nursery Stocks.
to 200 µL L-1 of chlorine. Downy mildew Agricultural Handbook 66, U.S. Department of
(Peronospora parasitica) produces purplish-red Agriculture, Washington, DC.
to brown surface lesions that become rough and
cracked in advanced cases, while the internal Maynard, D.N., and G.J. Hochmuth 1997. Knott’s
tissue can become grayish brown to black. Handbook for Vegetable Growers, 4th ed. Wiley,
Rhizoctonia root rot (Rhizoctonia solani) produces New York, NY.
lesions that are initially round and light brown
and can become slightly sunken. The tissue can Nonnecke, I.L. 1989. Vegetable Production/Radish,
become spongy. This disease favors high RH. pp. 339-346. Van Nostrand Reinhold, New York,
Avoid bruising, hydrocool to 4 °C (40 °F), and NY.
store at 0 to 2 °C (32 to 35 °F) to control these
diseases (Ryall and Lipton 1972a). Murry J. 1977. Fruit and vegetable facts and
pointers (radish). United Fresh Fruit and Vegetable
Association, Washington, DC.
Quarantine Issues
Ryall, A.L., and W.J. Lipton. 1972a. Handling,
There are no known quarantine issues. Transportation, and Storage of Fruits and
Vegetables, vol. 1. AVI, Westport, CT.
Suitability as Fresh-Cut Product Ryall, A.L., and W.J. Lipton. 1972b. Longer
storage-life for radishes. Agric. Res., vol. 20, no. 7.
Currently radishes are cut or diced in packaged
salad mixes and for food service, particularly for Saltveit, M.E. 1997. A summary of CA and MA
incorporation into salad bars. requirements and recommendations for harvested
vegetables. In M.E. Saltveit, ed., 7th International
Controlled Atmosphere Research Conference, July
References 13-18, 1997, University of California, Davis, vol.
4, pp. 98-117. University of California, Davis, CA.
AMS [USDA Agricultural Marketing Service].
1968. United States standards for grades of Thompson, H.C., and W.C. Kelly. 1957. Vegetable
radishes. At http://www.ams.usda.gov/AMSv1.0/ Crops. McGraw-Hill, New York, NY.
standards.
USDA [U.S. Department of Agriculture]. 1975.
Brooker, J., and J. L. Pearson. 1970. Planning How To Buy Food for Economy and Quality:
data for marketing selected fruits and vegetables Recommendations of the United States Department
in the south, part III, Fresh Vegetable Packing of Agriculture. Dover, New York, NY.
Handbook. Southern Cooperative Ser. Bull. 152,
Southern Association of Agricultural Experiment
Station Directors, Raleigh, NC.
526
Rambutan Grades, Sizes, and Packaging
Saichol Ketsa and Robert E. Paull There are no U.S. or International grade standards.
Fruit are sold in 2.25-kg (5-lb) and 4.5-kg (10-lb)
Ketsa is with the Department of Horticulture, one-piece fiberboard cartons. Sometimes fruit are
Kasetsart University, Bangkok, Thailand; Paull prepacked in punnets. In Southeast Asia, clusters
is with the Department of Tropical Plant and of fruit are sold in bunches still attached to the
Soil Sciences, University of Hawaii at Manoa, stem.
Honolulu, HI.
Precooling Conditions
Scientific Name and Introduction
Only room-cooling is recommended.
Rambutan fruit (Nephelium lappacium L.) are
large ovoid or globose fruit about 4.5 cm (1.8 in)
long and 2.5 to 3.7 cm (1 to 1.5 in) wide that grow Optimum Storage Conditions
on woody stalks in clusters of 10 to 18. The outer
skin is 2 to 4 mm (0.1 to 0.2 in) thick and covered Store at 8 to 15 °C (46 to 59 °F) with 90 to 95%
with soft, long spines (spinterns) that turn red or RH to achieve a storage life of 14 to 16 days.
yellow when ripe. The edible aril flesh is attached There may be changes in the skin and spine
to a single, large seed. The fruit is related to litchi coloration after storage, but the flesh is unaffected.
and longan (Nakasone and Paull 1998). Temperature recommendations vary for different
cultivars (O’Hare 1992). Fruit held at 20 °C (68
°F) with 60% RH last about 3 to 5 days.
Quality Characteristics and Criteria
Quality criteria include fruit size, shape, and Controlled Atmosphere (CA)
weight; bright skin and spine color; uniformity; Considerations
absence of defects; and freedom from disease and
insects. High SSC and low TA are desirable (Ketsa CA of 7 to 12% CO2 and 3 to 5% O2 at 10 °C (50
and Klaewkasetkorn 1992). Mechanical injury and °F) is recommended (Kader 1993). At 9 to 12%
dehydration are major causes of appearance loss. CO2, color loss is reduced and shelf-life extended
by 4 to 5 days, while low O2 (3%) has little effect
(O’Hare et al. 1994, O’Hare 1995). Raising
Horticultural Maturity Indices CO2 levels above 12% has no additional effect,
and decay can begin after a few weeks’ storage.
Skin and spine coloration is the main horticultural The MA/CA effect appears to derive more from
maturity index. Fruit having green skin and CO2 elevation and minimizing water loss than
greenish-red spines are sour. Ripe fruit have both through any effect of low O2. Storage in sealed
skin and spines red or yellow, depending on the polyethylene film bags or plastic containers is
variety. Between these two stages, sugar content effective in reducing water loss (Mendoza et al.
increases about 20% and acid level decreases by 1972, Mohamed and Othman 1988, Ketsa and
half (Mendoza et al. 1982). The acceptable stage is Klaewkasetkorn 1995), while wax coatings are
16 to 28 days after color break, at which time skin less effective (Mendoza et al. 1972, Lam and Ng
and spines are brightly colored (O’Hare 1992). 1982, Brown and Wilson 1988). A shelf-life of 14
Over-ripe fruit have a watery texture (Somboon to 21 days can be expected.
1984) which may be a senescence-induced tissue
breakdown.
527
Retail Outlet Display Considerations skinned cultivars often following heavy rains
during the last phase of fruit growth. Poor filling
Rambutan should be displayed in trays with a has been associated with poor nutrition and dry
clear film overwrap or in clam shell containers conditions just after flowering.
with no perforations at 10 to 12 °C (50 to 55 °F).
Fruit should not be misted or iced.
Postharvest Pathology
Chilling injury and darkening of spines and skin Farungsang, U., N. Farungsang, and S. Sangchote.
are major postharvest disorders. Darkening is 1991. Postharvest diseases of rambutan during
caused by dehydration and mechanical injury storage at 13 or 25 °C. In 8th Australasian Plant
(Landrigan et al. 1996). Preharvest disorders Pathology Society Conference, 7-11 October,
include skin splitting and poor filling of fruit 1991, Sydney, Australia. Sydney University,
(O’Hare 1992). Skin splitting occurs in thin- Sydney, Australia.
528
Kader, A.A. 1993. Modified and controlled O’Hare, T.J. 1992. Rambutan: postharvest
atmosphere storage of tropical fruits. In B.R. physiology and storage. Trop. Fruit News 26:4-6.
Champ, E. Highley and G.I. Johnson, eds.,
Proceedings of the International Conference on O’Hare, T.J. 1995. Postharvest physiology and
Postharvest Handling of Tropical Fruits, Chiang storage of rambutan. Postharv. Biol. Technol.
Mai, Thailand, July 1993, pp. 239-249. ACIAR 6:189-199.
Pub. no. 50. Australian Centre for International
Agricultural Research, Canberra, Australia. O’Hare, T.J., A. Prasad, and A.W. Cooke. 1994.
Low temperature and controlled atmosphere
Ketsa, S., and O. Klaewkasetkorn. 1992. storage of rambutan. Postharv. Biol. Technol.
Postharvest quality and losses of ‘Rongrein’ 4:147-157.
rambutan fruits in wholesale markets. Acta Hort.
321:771-777. Sangchote, S.,U. Farungsang, and N. Farungsang.
1992. Rambutan diseases. In G.I. Johnson and
Ketsa, S., and O. Klaewkasetkoan. 1995. Effect E. Highley, eds., Proceedings of the Workshop
of modified atmosphere on chilling injury and on Development of Postharvest Handling
storage-life of rambutan. Acta Hort. 398:223-231. Technology for Tropical Tree Fruits, July 1992,
Bangkok, Thailand, p. 51-59. Australian Center
Lam, P.F., and K.H. Ng. 1982. Storage of waxed for International Agricultural Research, Canberra,
and unwaxed rambutan in perforated and sealed Australia.
polyethylene bags. Report no. 251, Malaysian
Agricultural Research and Development Institute, Somboon, Y. 1984. Effect of Temperature and
Food Technology Division, Selangor, Malaysia. Maturity Stages on Biochemical Changes During
Storage of Rambutan (Nephelium lappaceum
Landrigan, M., S.C. Morris, D. Eamus, and W.B. Linn.) cv. Seechompoo and cv. Rongrien. M.S.
McGlasson. 1996. Postharvest water relationships thesis. Kasetsart University, Bangkok, Thailand.
and tissue browning of rambutan fruits. Sci. Hort.
66:201-208.
529
Raspberry Horticultural Maturity Indices
Raspberries (Rubus ideaus L.) are a member of Grades, Sizes, and Packaging
the Rosaceae family, grown as a perennial crop.
Raspberries are available commercially in red, Raspberries are graded as U.S. No. 1 or No. 2
yellow, purple, and black forms. The red or yellow based on freedom from mold, decay, sunscald,
raspberry is classified into two subspecies: R. over-ripeness, and injury. A limit of 1% of berries
idaeus subsp. vulgatus Arrhen. (European red for mold and 10% total for defects separates
raspberry) and R. idaeus subsp. strigosus Michx. No. 1 grade from No. 2. No. 2 fruit can have no
(American red raspberry). Black raspberries found more than 2% berries with decay. Trays holding
in eastern North America are R. occidentalis L.; 12 half pints (125 g), usually vented plastic
R. glaucus L. is a South American tetraploid black clamshell containers, are the standard package.
raspberry. Purple raspberries (R. neglectus Peck.) No minimum berry size is required.
result from crosses of black and red raspberries.
530
change, and growth of molds. Levels of CO2 Physiological Disorders
>20% can cause discoloration, softening, and off
flavor (Agar and Streif 1996). Shriveling (water loss), leakers (berries with
leakage of juice), and UV damage (white
drupelets) are the primary disorders found in
Retail Outlet Display Conditions raspberries.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Agar, I.T., and J. Streif. 1996. Effect of high CO2
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and and controlled atmosphere (CA) storage on the
1.8 at 20 °C (68 °F). To calculate heat production, fruit quality of raspberry. Gartenbauwissenschaft
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 61:261-267.
or by 61 to get kcal tonne-1 day-1.
531
Burdon, J.N., and R. Sexton. 1990. Fruit
abscission and ethylene production in red
raspberry cultivars. Sci. Hort. 43:95-102.
532
Rhubarb Precooling Conditions
Scientific Name and Introduction Rhubarb petioles can be stored for 2 to 4 weeks
at 0 °C (32 °F) with 95 to 100% RH (McGregor
Rhubarb, or “pie plant,” (Rheum rhabarbarum 1987).
L.) is a perennial of the Polygonaceae family.
Fleshy petioles are the edible portion. Petioles
may be green, pink, or white depending on the Controlled Atmosphere Considerations
variety. Field production in North America is
mainly in Washington, Oregon, Michigan, and CA storage has not yet been used for rhubarb.
Ontario. Rhubarb is forced in heated structures
in Washington, Michigan, and Ontario. Fresh
rhubarb is mostly available in late winter through Respiration Rates
spring (Foust and Marshall 1991), but limited
supplies are available at other times. Temperature mg CO2 kg-1 h-1
0 °C 9 to 13
5 °C 11 to 18
Quality Characteristics and Criteria 10 °C 25
15 °C 31 to 48
Petiole color is associated with quality. The order 20 °C 40 to 57
of preference is red, pink, and green. Petioles
should appear fresh with no signs of desiccation or To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
decay whether presented for sale intact or cut into by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
sections. 1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
Grades, Sizes, and Packaging
Quarantine Issues
References
534
Rutabaga Grades, Sizes, and Packaging
Maria Cecilia do Nascimento Nunes Grades include U.S. No. 1 and U.S. No. 2, based
on subjective external appearance. Sizes are
Nunes is formerly with the Air Cargo defined as Small, diameter of 5.1 to10.2 cm (2 to
Transportation Research Group, Department of 4 in); Small-Medium, 8.9 to 14 cm (3.5 to 5.5 in);
Soils and Agrifood Engineering, Laval University, Medium, 10.2 to 15.2 cm (4 to 6 in); and Large,
Quebec, Canada. She is now with the Food 13 to 17.8 cm (5 to 7 in). Common packaging is
Science and Human Nutrition Department, 23-kg (50-lb) bushel cartons or bags (about 20
University of Florida, Gainesville, FL. roots) and 11-kg (25-lb) 0.5-bushel cartons (about
10 roots).
536
Suitability as Fresh-Cut Product Franklin, E.W., and E.C. Lougheed. 1975. Storing
rutabagas. Factsheet 75-068, AGDEX 258/64,
Rutabagas are good candidates for sale in Ministry of Agriculture and Food Ontario, Guelph,
consumer-size packages. Prepeeled rutabagas Ontario, Canada.
packaged in consumer film bags keep in good
condition for 3 weeks at 0 °C (32 °F). Fresh-cut Gardner, J., and I.L. Nonnecke. 1987. Rutabagas
rutabagas stored in 15% O2 will keep for (table turnips). Factsheet 87-032, AGDEX 258/20,
10 days at 10 °C (50 °F) and 20 days at 1 °C Ministry of Agriculture and Food Ontario, Guelph,
(34 °F). Fermentation may occur at lower Ontario, Canada.
O2 levels (Alexander and Francis 1964). Storage
of cubed, shredded, or peeled rutabaga in 5% O2 Geeson, J.D., K.M. Browne, and H.P. Everson.
and 5% CO2 at 0 to 5 °C (32 to 41 °F) reduces 1989. Long-term refrigerated storage of swedes. J.
respiration rate and has a moderate potential Hort. Sci. 64(4):479-483.
(Gorny 1997).
Gorny, J.R. 1997. A summary of CA and MA
requirements and recommendations for fresh-
Special Considerations cut (minimally processed) fruits and vegetables.
In J.F. Gorny, ed., 7th International Controlled
Strong odors may be transferred to fruits and leafy Atmosphere Research Conference, July 13-18,
vegetables if they are held in the same storage area 1997, University of California, Davis, vol. 5, pp.
with rutabagas. 30-66. University of California, Davis, CA.
537
Salad Greens or in small sealed plastic bags, trays, or clamshell
containers. These greens may also be packed
Kimberly P. Wright as bunches of leaves or plants (Rubatzky and
Yamaguchi 1997, Péron and Rees 1998).
Wright is with Dole Fresh Vegetables, Salinas,
CA.
Precooling Conditions
Scientific Names and Introduction Greens for salads should be cooled to 0 °C (32 °F)
as soon as possible after harvest. Vacuum-cooling
Various greens other than lettuce are used in raw is effective for removing field heat.
salads. These include corn salad; lamb’s lettuce;
field salad; mâche (Valerianella locusta [L.]
Latterrade em. Betcke [V. olitoria]); dandelion Optimum Storage Conditions
(Taraxacum officinale Wiggers); French or round
sorrel (Rumex scutatus); garden sorrel (R. acetosa Salad greens should be stored at 0 to 2 °C (32
L.); miner’s lettuce; winter purslane; claytonia to 36 °F) with 95 to 100% RH. Rocket salad
(Montia perfoliata [Claytonia perfoliata]); typically lasts 7 to 10 days and other leafy greens
mizuna (Brassica rapa L. subsp. japonica group 10 to 14 days (Cantwell 1997). Top icing can be
Japonica); purslane (Portulaca oleracea L.); and used.
rocket salad, roquette, arugula, rucola, or rugula
(Eruca vesicaria [L.] Cav. subsp. sativa [Mill.]
Thell). Young leaves are generally used. Controlled Atmosphere (CA)
Considerations
Greens are harvested as individual leaves, leaf Use of water sprinklers is acceptable. Leafy greens
clusters, or whole plants and should be young, are highly susceptible to water loss and wilting.
tender, and mild flavored. Plants that have
flowered are usually too strong in flavor and tough
in texture for use in raw salads. Chilling Sensitivity
538
Ethylene Production and Sensitivity Quarantine Issues
Salad greens have very low ethylene production There are no known quarantine issues.
but are highly sensitive to ethylene exposure
(Cantwell 1997), which typically results in loss of
chlorophyll leading to yellowing of leaves. Suitability as Fresh-Cut Product
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Cantwell, M. 1997. Properties and recommended
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and conditions for storage of fresh fruits and
1.8 at 20 °C (68 °F). To calculate heat production, vegetables. At http://postharvest.ucdavis.edu/
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day 1 produce_information/storagefreshproduce.
or by 61 to get kcal tonne-1 day-1.
Cantwell, M.I., and M.S. Reid. 1993. Postharvest
physiology and handling of fresh culinary herbs. J.
Physiological Disorders Herbs, Spices and Medicinal Plants 1:93-127.
Because of the delicate texture of the leaves, de Leiris, J. 1987. The packaging of fresh
greens are highly susceptible to mechanical vegetables in barrier films and modified
damage, which may result in discoloration and atmospheres. In Proceedings of the 1st
decay. International Conference on Packaging Advances,
pp. 135-162. Nova-Pack, Dusseldorf, Germany.
539
Piergiovanni, L., P. Fava, and S. Ceriani. 1999. A
simplified procedure to determine the respiration
rate of minimally processed vegetables in flexible
permeable packaging. Ital. J. Food Sci. 11:99-110.
540
Salsify Grades, Sizes, and Packaging
Quality Characteristics and Criteria The skin is very delicate, and salsify easily loses
water if not in plastic-lined trays. Misting with
There are no U.S. or international standards. water is beneficial.
Taproots must be sound, clean, fresh, and without
any foreign smell or taste. They must be full-
bodied, straight, and unbranched. They should not Chilling Sensitivity
be woody. Color must be uniform light-yellow or
brown-black. Salsify is not chilling sensitive. It should be stored
as cold as possible without freezing.
541
Respiration Rates Hardenburg, R.E., A.E. Watada, and C.Y. Wang.
1986. Salsify. In R.E. Hardenburg, A.E. Watada,
Temperature mg CO2 kg-1 h-1 and C.Y. Wang, The Commercial Storage of
0 ºC 22 to 28 Fruits, Vegetables, and Florist and Nursery Stocks,
5 ºC 33 to 53 p. 69. Agricultural Handbook 66, U.S. Department
10 ºC 40 to 57 of Agriculture, Washington, DC.
20 ºC 193
Kierstan, M.P.J. 1978. Production of fructose
-1 -1 -1
To get mL CO2 kg h , divide the mg kg h rate -1 syrups from inulin-containing plants [Jerusalem
by 2.0 at 0 ºC (32 ºF), 1.9 at 10 ºC (50 ºF), and artichokes, chicory, salsify extracts]. Biotech.
1.8 at 20 ºC (68 ºF). To calculate heat production, Bioeng. 20:447-450.
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1. Stoll, K. 1974. Storage of vegetables in modified
atmospheres. Acta Hort. 38:13.
Physiological Disorders
Postharvest Pathology
Quarantine Issues
References
542
Sapodilla and Related Fruits Black sapote will tolerate irradiation at 0.15 kGy,
but abnormal ripening of some fruit is likely when
Elhadi M. Yahia treated at 0.3 kGy (Miller et al. 1997).
This chapter discusses black sapote, lucuma, The lucuma (lucumo, lucmo, lucma, rucma, or
mamey apple, sapodilla, sapote, star apple, and mamon) (Lucuma obovata, HBK) is round or
white sapote. ovate with a green surface and yellow, mealy
flesh. Fruit are about 7 cm long. The lucuma is a
Note regarding respiration data for Sapodilla and climacteric fruit. It has a low water content (64
related fruit: to 72%) and higher amounts of riboflavin, niacin,
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate and ascorbate than apples or bananas (Wenkam
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and and Miller 1965, Lopez 1984).
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU per ton per
day or by 61 to get kcal tonne-1 day-1. Horticultural Maturity Indices
Optimum Storage Conditions and Chilling A classification of five stages of maturity was
Sensitivity developed according to peel and pulp color,
texture, SSC, and respiration (Lizana 1980). The
Black sapote is chilling sensitive. Fruit held at classes in relation to peel and pulp color are—
15, 20, or 25 °C (59, 68, or 77 °F) for up to 7, 10, Class Peel color Pulp color
or 15 days and then transferred to 25 °C (77 °F) 1 light-yellow light-yellow
ripened normally (Miller et al. 1997). Fruit held 2 light-green creamy-yellow
at 10 °C (50 °F) for 7 days and then transferred 3 yellow-green yellow
to 25 °C (77 °F) also ripened normally. However, 4 green-yellow dark-yellow
some fruit held at 10 °C (50 °F) for 10 or 15 days 5 green-yellow orange-yellow
showed abnormal ripening, and most fruit stored
at 1 to 5 °C (34 to 41 °F) did not ripen normally or Fruit ripened on the tree usually become soft
failed to ripen regardless of storage duration. and very fragile (Lizana 1980). The pulp is very
dry when ripe (Lizana 1980). Intense respiratory
activity and sugar accumulation occur during
ripening (Lizana et al. 1986).
543
Mamey Apple oval—and contains one or several shiny black
seeds. It weighs about 70 to 300 g and has a
Scientific Name and Introduction dull brown color, thin skin, and yellowish, light
brown, or red pulp. Sapodilla fruit are prized for
Mamey apple, also know as mamey and zapote, a pleasant aroma and sweet taste. Fruit growth
(Mammea americana) is a fruit of about 300 follows a sigmoid pattern (Lakshminarayana and
to 500 g having a peachlike flavor. The fruit is Subramanyam 1966). Fruit are very susceptible to
a drupe about the shape and size of an orange, mechanical injury.
with a russet surface covered with small spots.
It is round with a thick, brown, leathery skin
containing one large single seed surrounded by a Horticultural Maturity Indices
thin layer of flesh. The tough rind is about 4 mm
thick. The flesh, or endocarp, is yellow, about 2 to The erratic flowering habit of sapodilla and the
5 mm thick, and fused with the testa. presence of fruit at all stages of development on
the tree make it difficult to determine optimum
harvest time (Lakshminarayana 1980). Fruit
Chilling Sensitivity harvested later than optimum time usually soften
very rapidly and become very difficult to handle.
Chilling injury symptoms include failure to ripen, Fruit harvested earlier than physiological maturity
accelerated softening, development of brown may not soften, are usually low in sweetness
spots in pulp, and development of off flavors and and high in astringency when ripe with a rather
aromas. unappealing alcoholic aftertaste, and form pockets
of coagulated latex that lower quality. Unripe fruit
are highly astringent and contain large amounts
Ethylene Production of leucoanthocyanidins. The sucrose content and
pulp-to-peel ratio increase during maturation
Ethylene production at 27 °C (81 °F) is as much (Pathak and Bhat 1953).
as 400 µL kg-1 h-1, among the highest of all fruits
(Akamine and Goo 1978). Fruits shed brown scaly external material and
become smooth when reaching physiological
maturity (Lakshminaryana 1980). Fruit ready for
Respiration Rates harvest do not show green tissue or latex when
scratched with a fingernail. Fully mature fruit
Mamey apple is a climacteric fruit. The respiration have brown skin and separate easily from the stem
rate at 27 °C (81 °F) is 28 to 40 mg (14 to 20 µL) without leaking latex. Extent of scurfiness is also a
CO2 kg-1 h-1 (Akamine and Goo 1978). good indicator of maturity (Kute and Shete 1995).
A fruit with a smooth surface, shining potato
color, and rounded stylar end is considered mature
Sapodilla (Kute and Shete 1995).
544
Optimum Storage Conditions Chilling Sensitivity
Postharvest life is 2 to 3 weeks at 12 to 16 °C (54 Sapodilla fruit are highly susceptible to chilling
to 61 °F) with 85 to 90% RH. Storage life is about injury. Storage of fruit at 6 to 10 °C (43 to 50 °F)
13 days at 25 °C (77 °F), 15 days at 20 °C (68 causes irreversible damage and results in poor
°F), and 22 days at 15 °C (59 °F) (Broughton and flavor (Broughton and Wong 1979, Salunkhe and
Wong 1979). Short-term holding of fruit—less Desai 1984). Chilling injury also occurred in fruit
than 10 h at 4 °C (39 °F)—before storage at 20 stored for 21 days at 10 °C (50 °F). However, fruit
°C (68 °F) extends storage life up to 24 days with waxed with a fatty acid sucrose ester kept for 40
satisfactory quality (Broughton and Wong 1979). days at 10 °C (50 °F).
Exposure of fruit to gamma irradiation at 0.1
kGy extended storage life by 3 to 5 days at 27 °C
(80 °F) and 15 days at 10 °C (50 °F) without any Ethylene Production and Sensitivity
effect on ascorbate content (Salunkhe and Desai
1984). Ethylene production is 2.8, 3.7 and 6.1 µL
kg-1 h-1 at 15, 20 and 25 °C (59, 68 and 77 °F),
respectively (Broughton and Wong 1979).
Modified Atmosphere (MA) and Controlled Treatment of sapodilla fruit with etherel at 1 to 3
Atmosphere (CA) Considerations mL L-1 accelerated ripening and reduced pectin
content, phenolic content, SSC, sugar content, and
Storage life of sapodilla is extended by use of vitamin C content (Shanmugavelu et al. 1971, Das
MA and removal of ethylene (Broughton and and Mahapatra 1977, Ingle et al. 1982). Removing
Wong 1979, Yahia 1998). Storage life at room ethylene delays ripening (Chundawat 1991).
temperature increased from 13 days to 18 days in
5% CO2, 21 days in 10% CO2, and to 29 days in
20% CO2. However, fruit held in 20% CO2 failed Respiration Rates
to ripen, and this level of CO2 is deleterious.
Sapodilla is a climacteric fruit (Lakshminaryana
‘Kalpatti’ fruit treated with 6% Waxol, with 250 and Subramanyam 1966, Broughton and Wong
or 500 µL L-1 Bavistin, or with hot water at 50 °C 1979) but does not reach the climacteric while
(122 °F) for 10 min and wrapped in 150 gauge on the tree (Lakshminaryana and Subramanyam
polyethylene film with 1% ventilation ripened 1966). The respiration rate at 24 to 28 °C
later than the controls, but fungal rot was high (75 to 82 °F) was 16 mg (9 µl) CO2 kg-1 h-1
(Bojappa and Reddy 1990). Fruit treated with (Lakshminaryana and Subramanyam 1966).
6% wax emulsion and packed in 200-guage Preharvest sprays of isopropyl n-phenylcarbamate
polyethylene covers containing ethylene and CO2 (IPC) at 100 µL L-1 retard respiration, while
absorbents had a shelf-life of 45 days at 12 °C maleic hydrazide at 0.5 to 1.0 mL L-1 accelerates it
(54 °F), 10 days more than controls (Chundawat (Lakshminarayana and Subramanyam 1966).
1991).
545
Insects that infest sapodilla fruit include from the stem. Poles with knifes at the end are
Nephopteryx engraphella Rag., fruit flies, and an also used to harvest fruit. Fruit should not be
unidentified borer (Kute and Shete 1995). The allowed to fall on the ground.
most troublesome fruit flies are the Mediterranean
(Ceratitis capitata, Wied.) and Mexican
(Anastrepha ludens, Loew.) species. Grades, Sizes, and Packaging
Star Apple
Quality Characteristics and Criteria
Scientific Name and Introduction
Inferior or improperly ripened mamey sapotes will
develop a pronounced squashlike flavor. The star apple (caimito, sweetsop, or anon)
(Chrysophyllum cainito L.) is an apple-sized fruit,
commonly round but sometimes ovate, heart-
Horticultural Maturity Indices and shaped, or conical. It has a smooth, waxy skin.
Harvesting The fruit appears as a star when cross-sectioned.
Fruit have a soft flesh and are yellowish green in
Fruit are harvested when the flesh begins to turn color with a mild, sweet flavor. The pulp is white
red and are mature when the newly exposed layer or creamy white with numerous embedded small,
has turned from green to pinkish-brown, orange, shiny, dark-brown seeds.
or red. Immature fruit fail to soften, and their pulp
will turn dark-brown and inedible. Harvesting
must be done carefully to avoid mechanical
damage. The fruit should be twisted until it breaks
546
Harvesting Postharvest Pathology
Fruit should be matured on the tree but picked The most important pests include the annona seed
before fully ripe. Fruit picked immature will be borer and the ambrosia beetle. The annona seed
astringent and contain a sticky white latex. Fruit borer lays eggs in the seeds of very young fruits.
left to ripen on the tree often split open, especially Insects develop in the seeds and emerge as adults
during the rainy season. when the fruit matures.
This fruit is slightly sensitive to chilling injury. Quality Characteristics and Criteria
Broughton, W.J., and H.C. Wong. 1979. Storage Lopez, C. 1984. Cultivation of the lucumo and its
conditions and ripening of chiku fruits Achras industrialization. Rev. Campesino 115:38-53.
sapota L. Sci. Hort. 10:377-385.
McGregor, B.M. 1987. Tropical Products
Chundawat, B.S. 1991. Postharvest handling and Transport Handbook. Agriculture Handbook 668,
marketing of sapota fruits. National Seminar on U.S. Department of Agriculture, Washington, DC.
Optimizing Production and Utilization of Sapota,
October 1991, Navsari, Gujarat, India. Miller, W.R., J.L. Shari, and E. Baldwin. 1997.
Quality of irradiated and non-irradiated black
Das, R.C., and S.K. Mahapatra. 1977. Effect of sapote (Diospyros digyna Jacq.) after storage and
growth substances and fungicidal wax emulsion ripening. Proc. Fla. State Hort. Soc. 110:215-218.
on ripening of sapota. Prog. Hort. 8:75.
Mohamed, S., B. Taufik, and M.N.A. Karim.
Ingle, G.S., D.M. Khedkar, and R.S. Dabhade. 1996. Effects of modified atmosphere packaging
1982. Ripening studies in sapota fruit. Indian Food on the physiological characteristics of ciku (Arcas
Packer 36:72-77. sapota L.) at various storage temperatures. J. Sci.
Food Agric. 70:231-240.
548
Pathak, S., and J.V. Bhat. 1952. Studies on the
microorganisms associated with Achras sapota. J.
Univ. Bombay 20(5):14-18.
549
Southern Pea Horticultural Maturity Indices
Penelope Perkins-Veazie and Blair Buckley Fresh-market southern peas are harvested when
the pods are at the mature-green stage (peas are
Perkins-Veazie is formerly with the South Central fully developed and the majority of the pods have
Agricultural Laboratory, Agricultural Research undergone a color change). It is acceptable to have
Service, U.S. Department of Agriculture, some pods that have not undergone a complete
Lane, OK. She is now with the Department color change (green with some purpling in the
of Horticultural Science, North Carolina State case of purplehulls) provided the peas are mature
University, NC Research Campus, Kannapolis, size. Shelled peas should be light green in color.
NC. Buckley is formerly with the Louisiana
State University Research Station, Calhoun, LA. Pea color is affected both by maturity and cultivar.
He is now with the Red River Research Station, Tan or white peas are perceived to be too mature
Lousiana State University, Bossier City, LA. (except for cream types). Blackeye cultivars
exhibit the most difficulty in determining when
to harvest for fresh use because the color of the
Scientific Name and Introduction black hilum does not fully develop until peak
maturity. The color will be purple to chocolate-
Southern pea, or cowpea, (Vigna unguiculata [L.] brown if harvested early. If southern peas are to be
Walp.) is a native of Africa and is adapted to hot, harvested for edible pod use, they must be selected
dry climates. The southern pea is not actually a when quite young and tender—no more than half
pea, and thus differs considerably from the green of the expected diameter of mature green pods—
or English pea (Pisum sativum) (Peirce 1987). unless it is a cultivar specifically developed for
Prized in the southern United States, southern edible pod use.
peas are rich in folic acid, fiber, and calcium.
Peas are distinguished by pod color, pea color and
pattern, and hilum color. Five groups of cultivars Grades, Sizes, and Packaging
are recognized on the basis of seed and pod
coloration and seed packing in pod. They include USDA grades of southern peas are U.S. No.
black-eyed (‘California Blackeye No. 5’), cream 1 (95% of pods must be at least 12.5 cm [5
(‘Elite’), purplehull (‘Pinkeye Purple Hull’), in] long), and U.S. Commercial (no minimum
crowder (‘Mississippi Silver’), and miscellaneous length). Shelled peas are marketed in 4.5- to
other colored, noncrowder peas (‘Dixielee’). 5.4-kg (10- to 12-lb) plastic bags (considered
‘Bettersnap’ was developed specifically for edible the equivalent of a shelled bushel) or in 0.45-kg
pods (Fery and Dukes 1995). (1-lb) bags. Some large operations package peas
in vacuum-packed 0.45- and 4.5-kg (1- and 10-
lb) bags. No grades exist for southern peas used
Quality Characteristics and Criteria as snap beans. Fresh southern peas may also be
sold in the hull by the bushel. A USDA bushel
Some cultivars have edible pods (similar to green is 11.4 kg (25 lbs); however, at the local market
beans) and should be harvested when the pods level the bushel weight varies widely from region
are 10 to 16 cm (4 to 6 in) long, flexible, and dark to region or from 7.3 to 13.6 kg (16 to 30 lbs).
green. Shelled peas should be a mature size, have Pods for shelling are packed primarily in mesh
smooth skin, and exhibit characteristic color. bags (cabbage sacks) or wooden bushel baskets
(becoming less common).
550
Precooling Conditions Ethylene Production and Sensitivity
In both shelled and unshelled states, peas are Ethylene production rate is unknown. Southern
very prone to decay if held at room temperature. peas are probably sensitive to ethylene with
Unshelled peas are best cooled using a forced-air effects characterized by yellowing of pods.
system. Contact with water greatly accelerates
their deterioration. Shelled peas should be blown
free of foreign material and then hydrocooled in Respiration Rates
a solution of 100 µL L-1 chlorine to remove heat
quickly, preserve green color, and slow microbial Temperature Whole pods Shelled peas
growth. Peas to be trucked for processing are ------mg CO2 kg-1 h-1--------
shelled into field bins where temperatures may 2 °C 13 to 36 26 to 33
reach 38 °C (100 °F) (Hardenburg et al. 1986). 5 °C 16 to 33 -
Peas start to yellow and decay after a few hours 20 °C 145 to 151 90 to 161
at 25 °C (77 °F). Flavor deterioration and off Data from P. Perkins-Veazie (unpublished)
flavor in shelled peas may be a problem if they from pods 15 to 23 cm long of cultivars ‘Excel,’
are held for as much as 7 h at 30 °C (86 °F) before ‘BetterSnap,’ ‘Early Scarlet,’ and ‘Pinkeye Purple
processing. Hull BVR.’
Southern peas in the pod can be held for 6 to 8 To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
days at 4 to 5 °C (39 to 41 °F) with 95% RH. by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
Without refrigeration, they remain edible only 1.8 at 20 °C (68 °F). To calculate heat production,
about 2 days and show extensive decay in 4 to multiply mg kg-1 h-1 by 220 to get BTU per ton per
6 days. Shelled peas should be held for no more day or by 61 to get kcal tonne-1 day-1.
than 24 to 48 h at 4 to 5 °C (39 to 41 °F) (Jenkins
1954).
Physiological Disorders
Controlled Atmosphere (CA) Brown spots, cracking, and seed-coat splitting are
Considerations problems with pods and peas.
Retail Outlet Display Conditions Very little research has been done on postharvest
pathogens of southern pea. Botrytis cinerea (gray
Peas should be kept under high RH and mold) can quickly develop on pods and shelled
refrigerated to slow decay and color loss. peas (Roland E. Roberts, Texas A&M, 2002,
personal communication).
Chilling Sensitivity
Quarantine Issues
Unknown. Southern peas are probably sensitive
when held at 5 °C (41 °F) or below. There are no known quarantine issues.
551
Suitability as Fresh-Cut Product
Special Considerations
References
552
Sprouts is 1.3 to 7.6 cm (0.5 to 3 in), alfalfa sprouts are
harvested after 1 to 2 days when length is 2.5 to
Jennifer R. DeEll 3.8 cm (1 to 1.5 in), and radish sprouts after 2 to 4
days when length is 1.3 to 2.5 cm (0.5 to 1 in).
DeEll is with the Ontario Ministry of Agriculture
and Food, Simcoe, Ontario, Canada.
Grades, Sizes, and Packaging
Scientific Name and Introduction There are no established USDA quality standards
for sprouts. Perforated film packaging helps
Sprouts are young seedlings just after seed maintain the quality of fresh sprouts by reducing
germination. The most common marketed water loss. Alfalfa sprouts are marketed in 112- or
sprout is mung bean (Vigna radiata L. Wilczek; 168-g (4- or 6-oz) containers with 12 containers
syn., Phaseolus aureus Roxb.). Other sprouts per case. Mung bean sprouts are marked in the
include alfalfa (Medicago sativa L.); buckwheat same size containers and in 2.3-kg (5-lb) open
(Fagopyrum esculentum Moench); green pea flats.
(Pisum sativum L.); kidney, pinto, and navy bean
(Phaseolus vulgaris L.); lentil (Lens culinaris
Medik.), mustard (Brassica nigra L. Koch; Precooling Conditions
Sinapis alba L.); onion (Allium cepa L.); radish
(Raphanus sativus L.); red clover (Trifolium Sprouts should be cooled immediately and held at
partense L.); soybean (Glycine max L. Merr.); 0 °C (32 °F). Vacuum-cooling, hydrocooling, and
watercress (Nasturtium officinale R.Br.); and forced-air cooling are common methods.
wintercress (Barbarea vulgaris R.Br.).
Optimum Storage Conditions
Quality Characteristics and Criteria
Sprouts are highly perishable and most last 5 to 10
Fresh mung bean sprouts have crisp white days at 0 °C (32 °F) with 95 to 100% RH. Mung
hypocotyls and yellow or green cotyledons. bean sprouts stored at 0, 2.5, 5 or 10 °C (32, 37,
Symptoms of deterioration include darkening 41 or 50 °F) reach the lower limit of marketability
of the root and cotyledons, development of after 8.5, 5.5, 4.5 and 2.5 days, respectively
dark streaks on the hypocotyl, and eventual (Lipton et al. 1981). The shelf-life of bean sprouts
development of sliminess, decay, and a musty held at 0 °C (32 °F), but exposed daily to 20 °C
odor. Sprouts vary in texture and taste: Some are (68 °F) for 30 min can be reduced by 50%. Alfalfa
spicy (such as radish and onion sprouts); others and radish sprouts stored at 0 °C (32 °F) with
are hardy and often used in Asian foods (such >95% RH had a shelf-life of 7, and 5 to 7 days,
as mung bean sprouts); while others are more respectively (Cantwell 1997).
delicate and used in salads and sandwiches to add
texture and moistness (such as alfalfa sprouts).
Controlled Atmosphere Considerations
553
Retail Outlet Display Considerations Darkening of the radicles and browning of the
cotyledons are other changes associated with the
Sprouts should be held close to 0 °C (32 °F). They deterioration of mung bean sprouts. Cotyledon
should not be misted. color in mung bean sprouts may blacken at low
temperatures, and this may be a symptom of
chilling injury (DeEll et al. 2000).
Chilling Sensitivity
Temperature mg CO2 kg-1 h-1 Sprouts have been associated with human
0 °C 23 pathogenic bacteria, such as Aeromonas
2.5 °C 29 hydrophila, Escherichia coli O157:H7, Listeria
5 °C 42 monocytogenes, Salmonella spp., and Bacillus
10 °C 96 cereus (Brackett 1999). Therefore, the importance
Data from Lipton et al. (1981). of proper storage and handling, along with good
worker hygiene and sanitation, cannot be stressed
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate enough.
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU per ton per Acknowledgments
day or by 61 to get kcal tonne-1 day-1.
Some information in this chapter is from the
International Sprout Growers Association website
Physiological Disorders at http://www.isga-sprouts.org.
555
Squash function of the type of squash and market demand.
Squash may be field-packed directly into shipping
T. Gregory McCollum containers or transported to the packinghouse in
field boxes or bulk bins for washing and sizing
McCollum is with the U.S. Horticultural Research before packing. Squash are packed in a variety of
Laboratory, Agricultural Research Service, U.S. containers including bushel baskets, wire-bound
Department of Agriculture, Fort Pierce, FL. wooden crates, and fiberboard boxes (McGregor
1987). A plastic liner should be used in all wooden
containers to prevent abrasion and retard water
Scientific Name and Introduction loss.
Tenderness and firmness are the major quality Summer squash are highly perishable and
characteristics. The surface of summer squash not suited for storage longer than 2 weeks
should be shiny; dullness is a sign of senescence. (Hardenburg et al. 1986). For maximum shelf-life,
Fruit should be firm and free of physical injury. summer squash should be held at 5 to 10 °C (41 to
Dark green types should be entirely green; 50 °F) with 95% RH.
yellowish areas are a sign of senescence. Water
loss results in a dull surface and loss of firmness.
Controlled Atmosphere (CA)
Considerations
Horticultural Maturity Indices
Storage in low-O2 atmospheres appears to be of
Summer squash are harvested up to 1 week after little or no value for zucchini squash (Mencarelli
anthesis, when they are still shiny. Small fruit et al. 1983, Leshuk and Saltveit 1990).
are more desirable than large fruit because small
fruit’s flesh and seeds are more tender and slightly
sweet. Retail Outlet Display Considerations
556
Chilling Sensitivity Physiological Disorders
Summer squash are chilling sensitive and should Summer squash are very susceptible to water loss.
not be exposed to temperatures below 5 °C (41 Shriveling may become evident with as little as
°F) (Ryall and Lipton 1979). However, variation 3% weight loss. Precooling and storage at high
in chilling tolerance among summer squash RH minimize weight loss. Squash can be waxed,
types is great (Sherman et al. 1987, Suslow and but only a thin coating should be applied. Waxing
Cantwell 1998). Chilled summer squash show provides some surface lubrication that reduces
surface pitting and decay rapidly at nonchilling chafing in transit. Summer squash skin is very
temperatures, though damage may be absent tender; skin breaks and bruises can be a serious
during refrigeration. Chilled fruit have increased source of water loss and microbial infection.
rates of water loss upon transfer to nonchilling
temperatures (McCollum 1989).
Postharvest Pathology
Ethylene Production and Sensitivity Decay caused by fungal and bacterial pathogens
can cause significant postharvest losses in summer
Summer squash produce low to moderate amounts squash. The incidence of decay increases in
of ethylene: 0.1 to 1.0 µL kg-1 h-1 at 20 °C (68 fruit that have physical injury or chilling stress.
°F). The rate of ethylene evolution is greatly Common postharvest diseases include alternaria
increased in fruit that have been held at chilling rot, bacterial soft rot, cottony leak, fusarium rot,
temperatures (McCollum 1989). Increased phythopthora rot, and rhizopus rot. Alternaria rot
yellowing may result if green-skinned summer can be especially pronounced following chilling
squash are exposed to ethylene (Ryall and Lipton injury.
1979).
Quarantine Issues
Respiration Rates
There are no known quarantine issues.
-1 -1
Temperature mg CO2 kg h
0 °C 24 to 26
5 °C 27 to 37 Suitability as Fresh-Cut Product
10 °C 65 to 68
15 °C 139 to 167 Summer squash is frequently sliced and marketed
20 °C 153 to 175 in foam trays overwrapped with polyethylene.
Data from Hardenburg et al. (1986).
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Special Considerations
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production, All types of summer squash are extremely tender
multiply mg kg-1 h-1 by 220 to get BTU per ton per and are injured by the slightest scratch, bruise, or
day or by 61 to get kcal tonne-1 day-1. scuff. Yellow and scalloped squash show scuffing
clearly because the ensuing darkening is obvious
on a light background. Summer squash should
be handled gently throughout marketing; sorters
and packers should wear cotton gloves to prevent
fingernail cuts.
557
References Suslow, T.V., and M. Cantwell. 1998. Squash
(soft rind). Recommendations for maintaining
AMS [USDA Agricultural Marketing Service]. postharvest quality. At http://postharvest.ucdavis.
1984. United States Standards for Grades of edu/produce_information.
Summer Squash. U.S. Department of Agriculture,
Agricultural Marketing Service, Washington, DC.
At http://www.ams.usda.gov/AMSv1.0/standards.
558
Strawberry half of each fruit showing pink or red color. U.S.
Combination grade is a mixture of U.S. No. 1 and
Elizabeth J. Mitcham 2, with at least 80% meeting U.S. No. 1 grade.
Strawberries are often packaged by pickers in the
Mitcham is with the Department of Plant Sciences, field either into open-mesh baskets or into clear
University of California, Davis, CA. clamshell containers of 1 dry pint or 1 dry quart
capacity. The mesh baskets or clamshells are held
in a corrugated fiberboard tray holding about 4 to
Scientific Name and Introduction 5 kg (9 to 11 lb).
559
Chilling Sensitivity postharvest. The fungus continues to grow at 0 °C
(32 °F), but growth is slow at this temperature.
Strawberry fruit are not sensitive to chilling and Rhizopus rot, caused by Rhizopus stolonifer, is
should be stored as cold as possible without another important disease of strawberry. This
freezing. fungus cannot grow at temperatures <5 °C (41
°F). Postharvest fungicides are not used on
strawberries; therefore, prompt cooling, storing at
Ethylene Production and Sensitivity 0 °C (32 °F), preventing injury, and shipping at 0
°C (32 °F) under high CO2 are the best methods
Strawberries produce very low amounts of for disease control. Damaged fruit should be
ethylene: <0.1 µL kg-1 h-1 at 20 °C (68 °F). They eliminated from baskets to prevent spread of the
do not respond to ethylene (Mason and Jarvis disease to healthy berries (nesting) (Sommer et al.
1970). Removal of ethylene from storage air may 1973).
reduce disease development (El-Kazzaz et al.
1983).
Quarantine Issues
Disease is the greatest cause of postharvest loss. Ceponis, M.J., R.A. Cappellini, and G.W.
The most common decay is botrytis rot, also called Lightner. 1987. Disorders in sweet cherry and
gray mold, caused by Botrytis cinerea (Ceponis strawberry shipments to the New York market,
et al. 1987). This disease can begin preharvest, 1972-1984. Plant Dis. 71:472-475.
remaining as a latent infection; or it can begin
560
El-Kazzaz, M.K., N.F. Sommer, and R.J. Wells, J.M., and M. Uota. 1970. Germination and
Fortlage. 1983. Effect of different atmospheres growth of five fungi in low-oxygen and high-
on postharvest decay and quality of fresh carbon dioxide atmospheres. Phytopathology
strawberries. Phytopathology 73:282-285. 60:50-53.
Gil, M.I., D.M. Holcroft, and A.A. Kader. 1997. Wright, K.P., and A.A. Kader. 1997. Effect of
Changes in strawberry anthocyanins and other slicing and controlled-atmosphere storage on the
polyphenols in response to carbon dioxide ascorbate content and quality of strawberries and
treatments. J. Agric. Food Chem. 45:1662-1667. persimmons. Postharv. Biol. Technol. 10:39-48.
561
Sweet Corn starchy while sh2 varieties eventually taste watery
and bland.
Jeffrey K. Brecht
562
to that of traditional sweet corn varieties and lose only 6% is converted at 0 °C (32 °F). While
sugar as rapidly (Evensen and Boyer 1986, Olsen sh2 varieties lose sugar at the same rate as su1
et al. 1991), so cooling is still critical with these varieties, their higher initial sugar levels keep
newer varieties. Sweet corn should not be handled it sweet-tasting longer. For sh2 varieties, water
in bulk unless copiously iced because it tends to loss and pericarp toughening supplant loss of
heat throughout the pile. sweetness in limiting postharvest life (Brecht et
al. 1990). The former is minimized by prompt
Vacuum-cooling can adequately precool sweet cooling, trimming flag leaves and long shanks, and
corn, but the corn must be first wetted (and top- maintaining high RH, usually by icing. Denting
iced after cooling) to minimize water loss from of kernels is promoted by water loss from husk
husks and kernels (Showalter 1957, Stewart and leaves (Showalter 1967). A loss of 2% moisture
Barger 1960). Crated sweet corn can be vacuum- may result in objectionable kernel denting.
cooled from about 30 °C (86 °F) to 5 °C (41 °F) Pericarp toughening can also be minimized by
in 30 min. Hydrocooling by spraying, showering, prompt cooling and by maintaining sweet corn at
or immersion in water at 0 to 3 °C (32 to 38 °F) 0 °C (32 °F). Under optimum storage conditions,
is effective, though it takes longer than vacuum- the potential postharvest life of sh2 sweet corn is
cooling if the sweet corn is packed. Bulk sweet >2 weeks.
corn takes about 60 min to cool from 30 to 5 °C
(86 to 41 °F) in a well-managed hydrocooler,
while crated sweet corn takes about 80 min Controlled Atmosphere (CA)
(Talbot et al. 1991)—and few if any operators Considerations
leave it in that long. Periodic monitoring of sweet
corn temperature is needed to ensure proper Increased attention for CA and MAP was spurred
cooling to at least 10 °C (50 °F). Hydrocooling by an interest in marine transport to export sweet
nomographs for bulk and crated sweet corn are corn from the United States to Europe and the Far
available (Stewart and Couey 1963). East, which can involve transit times of >2 weeks.
Injurious atmospheres at 2 °C (35 °F) contain <2%
After hydrocooling, top-icing is desirable during O2 or >15% CO2 (Spalding et al. 1978), resulting
transport or holding to continue cooling, remove in fermentation, off flavors, and odors. Reduced
the heat of respiration, and keep the husks fresh. O2 and elevated CO2 reduce respiration and slow
When precooling facilities are not available, sucrose loss; elevated CO2 also reduces decay and
sweet corn can be cooled with package ice and maintains green husk color (Spalding et al. 1978,
top ice. Injection of an ice-water slurry (slush ice) Schouten 1993, Aharoni et al. 1996).
into cartons was as effective as hydrocooling and
better than vacuum-cooling in maintaining quality
(Talbot et al. 1991), probably due to residual ice in Retail Outlet Display Considerations
the cartons, since the cooling rate was slower than
for the other methods. Sweet corn should be displayed in refrigerated
cases or with ice.
Optimum Storage Conditions
Chilling Sensitivity
Traditional sweet corn varieties are seldom stored
for more than a few days because of the resulting Sweet corn is not chilling sensitive. It should be
serious deterioration and loss of tenderness and stored as cold as possible without freezing.
sweetness. The loss of sugar is about 4 times as
rapid at 10 °C (50 °F) than at 0 °C (32 °F). At 30
°C (86 °F), 60% of the sugar in su1 sweet corn
can be converted to starch in a single day, while
563
Ethylene Production and Sensitivity Quarantine Issues
Sweet corn produces only trace ethylene, and There are no known quarantine issues.
exogenous ethylene is not a problem, though high
ethylene amounts can lead to husk yellowing
given sufficient exposure time. Suitability as Fresh-Cut Product
There are no significant disorders. Brecht, J.K., S.A. Sargent, R.C. Hochmuth,
and R.S. Tervola. 1990. Postharvest quality of
supersweet (sh2) sweet corn cultivars. Proc. Fla.
Postharvest Pathology State Hort. Soc. 103:283-288.
Decay is not usually a serious problem, but when Evensen, K.B., and C.D. Boyer. 1986.
present it typically occurs on the husk and silks. Carbohydrate composition and sensory quality of
Trimming ears can promote decay development on fresh and stored sweet corn. J. Amer. Soc. Hort.
the cut kernels and other damaged tissues mainly Sci. 111:734-738.
caused by Alternaria alternata (Fr.) Keissler,
Fusarium moniliforme Sheldon, and Mucor Olsen, J.K., R.E. Giles, and R.A. Jordan. 1991.
hiemalis Wehmer (Aharoni et al. 1996). Thus, Postharvest carbohydrate changes and sensory
proper sanitation and temperature management quality of three sweet corn cultivars. Sci. Hort.
are important to minimize decay in trimmed sweet 44:179-189.
corn.
Risse, L.A., and R.E. McDonald. 1990. Quality
of supersweet corn film-overwrapped in trays.
HortScience 25:322-324.
564
Robinson, J.E., K.M. Browne, and W.G. Burton. Wann, E.V., C.Y. Lee, M.C. Bourne, and D.L.
1975. Storage characteristics of some vegetables Downing. 1997. Sweet corn. In D. Smith, J. Cash,
and soft fruits. Ann. Appl. Biol. 81:339-408. W. Nip, and Y. Hui, eds., Processing Vegetables:
Science and Technology, pp. 325-354. Technomic,
Scholz, E.W., H.B. Johnson, and W.R. Buford. Lancaster, PA.
1963. Heat evolution rates of some Texas-grown
fruits and vegetables. J. Rio Grande Valley Hort.
Soc. 17:170-175.
565
Sweetpotato Grades, Sizes, and Packaging
566
Radiation Treatments Chilling Sensitivity
Extension of sweetpotato shelf-life via treatment Sweetpotato roots freeze at -1.9 °C (28.6 °F)
with gamma irradiation in general offers no (Whiteman 1957) and are susceptible to chilling
advantage over proper storage temperature injury when stored at <12 °C (55 °F) (Lewis and
management. Morris 1956, Picha 1987). Symptoms of chilling
injury include root shriveling, surface pitting,
abnormal wound periderm formation, fungal
Optimum Storage Conditions decay, internal tissue browning, and hardcore
formation (Buescher et al. 1975a, Daines et al.
Following curing, sweetpotatoes should be 1976). Synthesis of chlorogenic acid and other
carefully moved, usually in palletized containers, phenolic compounds has been associated with
to a separate storage room and held at 14±1 °C tissue browning symptoms (Walter and Purcell
(57±2 °F) with 90% RH (Kushman 1975). Long- 1980).
term storage experiments have shown that roots
can be stored successfully under these conditions Hardcore is a physiological disorder in which
for up to 1 year without sprouting (Picha 1986), various areas within the root become hard,
though sensory quality declines with extended apparently due to cold-induced alterations in
storage. Storage room air flow should be 1,125 L cellular membranes (Yamaki and Uritani 1972).
min-1 tonne-1 (36 ft3 min-1 ton-1) of roots at optimal The disorder is not apparent in fresh roots but
temperature and RH. Storage at 19 °C (66 °F) appears after cooking or processing. All cultivars
or above results in considerable sprouting after appear to be susceptible to hardcore; however,
several months of storage and an associated loss in there is substantial variation in susceptibility
root quality and marketability. Storage of roots at among cultivars, and noncured roots appear to be
<12 °C (55 °F) results in chilling injury. more susceptible than cured roots.
567
to ambient ethylene that would normally not pathogens are typically opportunistic pathogens
be encountered during storage with proper that require wounds to gain entry into the root.
ventilation. Therefore, ethylene exposure under
normal storage conditions is a relatively minor Internal cork is a virus-mediated disorder in
concern. which root tissue develops necrotic lesions during
storage (Kushman and Pope 1972). The number
and size of lesions varies widely and increases
Physiological Disorders with storage duration and elevated storage
temperature. Lesions are found primarily in the
In addition to chilling-induced hardcore, the interior, but may also be present on the surface.
sweetpotato is susceptible to other physiological
disorders. Roots may be lost during curing Control of postharvest diseases centers on
or storage because of exposure to anaerobic prevention, since little can be done once the
conditions before harvest caused by excessive root is infected. During harvest, care must be
moisture (Ahn et al. 1980, Chang et al. 1982). taken to minimize damage to roots and exercise
Roots may appear sound, only to decompose proper sanitation. After harvest, roots should
rapidly once in storage, emitting a distinctive be cured immediately and then stored at the
sour, fermented odor. Pithiness is another proper temperature. Creating entry wounds via
disorder found in apparently sound roots; it is mechanical damage during movement from the
characterized by reduced density and a spongy curing room to storage areas should be judiciously
feel when squeezed. Curing and storage conditions avoided, as well as after storage during washing,
that promote a high metabolic rate facilitate sorting, and grading prior to marketing. Wash-
development, as do sprouting in storage and water should be frequently changed to prevent
exposure to low soil temperatures of 5 to 10 °C accumulation of inoculum, and the use of calcium
(41 to 50 °C) before harvest. hypochlorite in the water is recommended.
Postharvest pesticides, if used, must be applied in
accordance to State and Federal laws.
Postharvest Pathology
568
production areas can be shipped throughout the Daines, R.H., D.F. Hammond, N.F. Haard, and
continental United States, but they may not be M.J. Ceponis. 1976. Hardcore development
imported because of the risk of viral diseases. in sweet potatoes. A response to chilling and
its remission as influenced by cultivar, curing
The sweetpotato weevil is the single most temperatures and time and duration of chilling.
devastating insect pest of the crop worldwide and Phytopathology 66:582-587.
is a pest in both field and storage. To date, there
are no adequate field or storage control measures, Delate, K.M., and J.K. Brecht. 1989. Quality of
though CA storage treatments may have promise tropical sweetpotatoes exposed to controlled-
(Delate and Brecht 1989). Roots should not be atmosphere treatment for postharvest insect
shipped from weevil-infested production sites to control. J. Amer. Soc. Hort. Sci. 114:963-968.
other areas of the country.
Kays, S.J., and S.E. Kays. 1998. Sweetpotato
chemistry in relation to health. In Sweetpotato
Suitability as Fresh-Cut Product Production Systems toward the 21st Century,
pp. 231-272. Kyushu National Agricultural
No current potential. Experiment Station, Miyakonojo, Japan.
569
Picha, D.H. 1987. Chilling injury, respiration and
sugar changes in sweet potatoes stored at low
temperature. J. Amer. Soc. Hort. Sci. 112:497-502.
570
Swiss Chard Precooling Conditions
Physiological Disorders
Postharvest Pathology
Quarantine Issues
References
572
Tamarillo Grades, Sizes, and Packaging
The best maturity index for tamarillo is peel and Retail Outlet Display Considerations
pulp color. Other indices correlated with skin color
are changes in firmness, juice content, and SSC. Fruit should be kept cool and dry throughout
For dark-red-skinned types, which progress from marketing.
green to dark purple (color due to chlorophyll and
anthocyanins) to red, harvesting at the dark purple
stage is considered best. If fruit are harvested Chilling Sensitivity
green, flavor score, juice content, SSC, and
color of ripened fruit are inferior to those of fruit Tamarillos are sensitive to chilling injury if stored
harvested at the dark-purple stage (Heatherbell et below 3 °C (37 °F). Symptoms include pitting and
al. 1982, El-Zeftawi et al. 1988). a scaldlike browning of the skin, calyx, and stem.
Discoloration on the peel is observed within 15
days of storage at 0 °C (32 °F) (Espina and Lizana
1991).
573
Ethylene Production and Sensitivity Suitability as Fresh-Cut Product
Ethylene production is very low (<0.1 µL kg-1 h-1) No current potential exists.
until fruit begin to senesce (Pratt and Reid 1976).
Green and partially ripe fruit respond to ethylene
with increased respiration (Pratt and Reid 1976) References
and accelerated red color development (Prohens
et al. 1996). Green fruits, however, have less color Blank, R.H., H.M. Dance, R.E. Hampton, et al.
development and lower SSC:TA ratio when ripe 1987. Tamarillo (Cyphomandra betacea): effect of
than fruit harvested partially ripe (Prohens et al. field-applied fungicides and postharvest fungicide
1996). dips on storage rots of fruit. N.Z. J. Exp. Agric.
15:191-198.
574
Tamarind Quality Characteristics and Criteria
Elhadi M. Yahia Both green immature pods and brown ripe pods
are normally marketed when 5 to 20 cm (2.0 to 7.9
Yahia is with the Facultad de Quimica, in) long.
Universidad Autonoma de Queretaro, Queretaro,
Mexico.
Horticultural Maturity Indices
Scientific Name and Introduction Tamarind fruit take about 8 mo from fruit set
to harvest, and growth is a typical sigmoid type
Tamarind fruit (Tamarindus indica L.; synonyms (Hernandez-Unzon and Lakshminarayana 1982a).
T. occidentalis Gaertn., T. officinalis Hook.) As pods mature, skin develops into a brown,
are indehiscent, beanlike, curved pods 3 to brittle shell, the pulp turns brown or reddish-
20 cm (1.2 to 7.9 in) long weighing 15 to 20 brown, and seeds become covered with dry and
g (0.5 to 0.7 oz). Fruit have a scurfy brown, sticky pulp. When fully ripe, the shells are brittle
woody, fragile shell with brown pulp and 8 to 10 and easily broken. Mature fruit can be left on the
blackish-brown, hard, shiny seeds (Hernandez- tree for more than 6 mo after ripening without
Unzon and Lakshminarayana 1982a). Fruit are significant spoilage; however, birds and insects
about 30% shell, 30% pulp, and 40% seeds. The become pests. Fruit should be harvested when the
color of the pulp comes from the presence of moisture content is <20% to facilitate separation
several anthocyanins, of which vitexene is the of the shell from the pulp.
most important (Lewis and Neelakantan 1964).
There are also fruit with red pulp; these are not
commonly cultivated. However, the reddish-flesh Harvesting
types are distinguished in some regions and are
regarded as superior in quality. Fruit are eaten at Ripe fruit should be harvested so as to prevent
the green-mature stage or when the shell pod has improper ripening and difficulties in separation of
become brittle and the pulp brown. Tamarind is the peel after harvest. Fruit can be pulled off the
a good source of calcium, phosphorous, and iron peduncle or cut using scissors (Hernandez-Tuzon
and an excellent source of riboflavin, thiamin, and Lakshminarayana 1982b). Fruit for immediate
and niacin; but it contains only small amounts of processing are harvested by pulling pods away
vitamins A and C (Bueso 1980). from the stalk. Some can be harvested by shaking
the branches, leaving the remaining fruit to fall
Tamarind is of minor importance in the United naturally when ripe. In humid climates, fruit are
States. The two major types are “sweet-fruited” readily attacked by beetles and fungi and should
and “acid-flavored.” Tamarind is considered to be therefore be harvested before they are fully ripe.
both the sweetest and the most sour of all fruits— Dry, ripe fruits are easily cracked, and the pulp
on a fresh weight basis, mature tamarind pulp and fibers separated from the broken shell.
has 30 to 35% sugar and 12 to 24% TA. Some
cultivars have sweeter pulp, such as ‘Makham
Waan’ in Thailand and ‘Manila Sweet’ in Florida Optimum Storage Conditions
(Morton 1958).
The high SSC:TA and the low water content
contribute to a long storage life. Tamarind can be
stored with the skin or as a separated dry pulp.
Tightly packaged pods can be stored at about 20
°C (68 °F) for several weeks. The pulp of mature
tamarind is commonly compressed and packed in
575
palm leaf mats or plastic bags and stored at 20 °C Special Considerations
(68 °F). It can be stored for a significant length of
time when processed into a paste. It can be frozen Fruit are commonly processed into juices, nectars,
and stored for 1 year, or refrigerated for up to 6 fruit punch, concentrates, and glaceéd and
mo. During storage, the dry, dark-brown pulp crystallized fruit. The pulp can withstand thermal
becomes soft, sticky, and almost black. The pulp processing without affecting the original flavor
can be stored for a longer period after drying or profile (Bueso 1980).
steaming.
References
Respiration Rates
Bueso, C.E. 1980. Soursop, tamarind and chironja.
Tamarind fruit are nonclimacteric. Maximum In S. Nagy and P.E. Shaw, eds., Tropical and
CO2 production occurs 4 weeks after fruit set and Subtropical Fruits: Composition, Properties and
then gradually declines (Hernandez-Unzon and Uses, pp. 375-406. AVI, Westport, CT.
Lakshminarayana 1982b).
Hernadez-Unzon, H.Y., and S. Lakshminarayana.
1982a. Biochemical changes during development
Postharvest Pathology and ripening of tamarind fruit (Tamarindus indica
L.). HortScience 17:940-942.
Tamarind fruit are very tolerant to pathogens
and insects, except for occasional incidence of Hernadez-Unzon, H.Y., and S. Lakshminarayana.
scab. This resistance may be due to the low water 1982b. Developmental physiology of tamarind
content and high acid and sugar content, as well as fruit (Tamarindus indica L.). HortScience 17:938-
high polyphenol content in the peel. Ripe fruit are 940.
susceptible to mold, insects, and birds.
Lewis, Y.S., and S. Neelakantan. 1964. The
real nature of tamarind anthoxanthin. Curr. Sci.
Quarantine Issues 15:460.
Various weevils and borers can infest the ripening Morton, J.F. 1958. The tamarind, its food,
pods or stored fruits. Pulp separated from the peel medicinal and industrial uses. Proc. Fla. State
is highly susceptible to molds. Tamarind beetle Hort. Soc. 79:355-366.
(Pacymerus [Coryoborus] gonogra) and tamarind
seed borer (Calandra [Sitophilus] lineris) can
infest ripening pods and persist in the stored fruits.
The rice weevil (Sitophilus oryzae), rice moth
(Corcyra cepholonica), and fig moth (Ephestia
cautella) can infest fruit in storage.
576
Taro harvested and smaller coromels removed; diseased
areas on main corms are excised. In eddoe, the
coromels are also harvested. Young taro leaves are
Robert E. Paull and Ching Cheng Chen
also harvested, bunched, and marketed as a leafy
vegetable.
Paull and Chen are with the Department of
Tropical Plant and Soil Sciences, University of
Hawaii at Manoa, Honolulu, HI.
Grades, Sizes, and Packaging
577
Chilling Sensitivity Suitability as Fresh-Cut Product
Chilling injury leads to pitting and increased Taro must be cooked before eating.
postharvest disease.
Special Considerations
Ethylene Production and Sensitivity
Corms are roasted, baked, boiled, or deep-fried.
Taro roots have a very low ethylene production. Grated, cooked corm is sometimes mixed with
There is no known response of taro roots to coconut milk. Corms are boiled, mashed, and
ethylene application. sieved for poi.
Respiration rates of taro have not been O’Hare, S.K., and M.P. Asokan. 1986. Edible
documented. aroids: botany and horticulture. Hort. Rev. 8:43-
99.
Pythium root rot can be a major problem in Snowdon, A.L. 1992. Tropical roots and tubers—
wetland taro. Corm rots can also be associated cocoyams (tannias and taros). In A.L. Snowdon,
with a complex of microorganisms, including Color Atlas of Postharvest Diseases and Disorders
Fusarium, Sclerotinia, Erwinia, Botryodiplodia, of Fruits and Vegetables, vol. 2, Vegetables, pp.
and Ceratocystis. These decay organisms are 350-357. CRC Press, Boca Raton FL.
associated with field infection through wounds.
After washing roots to remove soil and then
cutting out diseased tissue, corms should be dried
(cured) so that wounds can heal. Curing is best
done at 20 to 30 °C (68 to 86 °F), followed by
cooling to control further disease development
(Snowdon 1992).
Quarantine Issues
578
Tomatillo Grades, Sizes, and Packaging
Marita Cantwell There are no U.S. Grades. Fruit are not usually
sized before packing. They are packed in 18-kg
Cantwell is with the Department of Plant Sciences, (40-lb) crates (Mexican fruit) or in 4.5-kg (10-lb)
University of California, Davis, CA. cartons (U.S. fruit).
The tomatillo, or husk tomato, (Physalis ixocarpa Forced-air cool or room-cool to retain fresh
Brot. ex Hornem) is a warm-season vegetable appearance of the husk.
of the Solanaceae family. The small, spherical,
green or green-purple fruit is surrounded by an
enlarged calyx, or “husk.” As the fruit matures, Optimum Storage Conditions
it fills the husk and can split it open by harvest
time. Tomatillo is the key ingredient in fresh and Tomatillos can be stored under a wide range
cooked green salsas and other Latin American of conditions. At ambient temperatures, the
dishes. Tomatillos are available year-round. In the husks will dry, but the fruit will remain in good
United States tomatillo are produced mainly on condition for about 1 week. The freshness of fruit
small acreages in California, but large volumes are and husk can be extended by storage at 5 to 10 °C
imported from Mexico (Smith et al. 1999). (41 to 50 °F) with 80 to 90% RH. Chilling injury
can occur after 3 weeks at 5 °C (41 °F).
579
Ethylene Production and Sensitivity been identified. Washing in chlorinated water
reduces superficial mold growth, but this may be
Immature tomatillos produce low amounts of difficult to implement commercially since it is
ethylene: 0.5 to 2.0 µL kg-1 h-1 at 10 to 20 °C difficult to remove all moisture inside the husk.
(50 to 68 °F). More mature fruit produce greater
amounts: 1 to 10 µL kg-1 h-1. Ethylene production
can be high, 20 to 40 µL kg-1 h-1 at 20 °C (68 °F), Quarantine Issues
in overmature fruit (that is, fruit showing color
changes due to ripening). Exposure of mature There are no known quarantine issues.
fruit to ethylene causes undesirable color changes
(Cantwell et al. 1992).
Suitability as Fresh-Cut Product
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Moriconi, D.N., M.C. Rush, and H. Flores.
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and 1990. Tomatillo: a potential vegetable crop for
1.8 at 20 °C (68 °F). To calculate heat production, Louisiana. In J. Janick and J.E. Simon, eds.,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day‑1 Advances in New Crops: Research, Development,
or by 61 to get kcal tonne-1 day-1. Economics, pp. 407-413. Timber Press, Portland,
OR.
Postharvest Pathology
580
Tomato of immature tomatoes (M-1) in lots of green-
harvested tomatoes can range from 20 to 80%
(Sargent and VanSickle 1996). Tomatoes harvested
Steven A. Sargent and Celso L. Moretti
at the mature-green stage (M-3 or M-4) will ripen
to high quality if handled properly (Maul et al.
Sargent is with the Horticultural Sciences
1998). Tomatoes harvested at the M-2 stage will
Department, University of Florida, Gainesville,
ripen to moderate quality, while those harvested
FL; Moretti is with EMBRAPA, National
at M-1 stage will not ripen to acceptable levels of
Vegetable Crop Research Center, Brasilia, Brazil.
quality.
Grades for field-grown tomatoes include U.S. Greenhouse-grown tomatoes are generally
No. 1, U.S. , No. 2, U.S. Combination, and No. 3, harvested at turning stage or later and packed
with tolerances established for defects at shipping according to ripeness and size (count) in single-
point and en route or at destination (AMS 1991). or double-layer cartons, with or without bottom
Fruit within a grade should have similar varietal trays. Cluster tomatoes are usually picked when
characteristics and be uniformly mature, not the least mature tomato begins to show red
overripe or soft, clean, well developed, fairly color. Uniformity of fruit color, stem freshness,
well formed, fairly smooth, and uniform in and fruit attachment to the stem are important
color. Fruit should be free from decay, freezing quality characteristics for this tomato type.
injury, sunscald, and damage caused by bruising, Cluster tomatoes are usually packed in single-
discoloration, sunken scars, cuts and broken layer cartons but sometimes in netted bags. Foam
skins, puffiness, catfaces, other scars, radial or bottom pads may be inserted to reduce abrasion
concentric growth cracks, and hail or insect injury. and bruising.
Tomatoes are rigorously sized with a 0.8 mm There are currently no U.S. grade standards for
(1/32-in) overlap permitted between sizes, other types of tomatoes. They are all typically
according to the following dimensions (AMS harvested once ripening has started and are sorted
1991): by defect and color. Roma-type tomatoes are
normally packed in 11.4-kg (25-lb) cartons by
Size Minimum Maximum color, while cherry, grape, and minipear tomatoes
diameter1 diameter2 are packed in 227- to 454-g (8- to 16-oz) baskets
Small 5.40 cm (2 4/32 in) 5.79 cm (2 9/32 in) or clamshell containers and placed in larger master
Medium 5.72 cm (2 8/32 in) 6.43 cm (2 17/32 in) cartons to facilitate palletizing.
Large 6.35 cm (2 16/32 in) 7.06 cm (2 25/32 in)
Extra 7.00 cm (2 24/32 in)
large Precooling Conditions
1
Will not pass through a round opening of the
designated diameter when tomato is placed Following commercial packing, tomatoes are
with the greatest transverse diameter across the routinely palletized and cooled to 20 °C (68 °F)
opening. for ripening or to 12 °C (54 °F) for storage. While
2
Will pass through a round opening of the room-cooling is common, forced-air cooling is
designated diameter in any position. more uniform and produces better quality fruit.
Packed, palletized tomatoes with pulp temperature
of 28 °C (83 °F) actually increased 2 °C (4 °F)
582
immediately after being stored at 20 °C (68 Ripeness stage Temperature O2(%) CO2(%) Benefit
°F), and only cooled to 23 °C (73 °F) after 24 Mature green 12 to 20 °C 3 to 5 2 to 3 slight
h using room-cooling (Brecht 1996). However, Red 10 to 15 °C 3 to 5 3 to 5 moderate
with forced-air cooling, tomatoes cooled to 20 Data from Saltveit (1997).
°C (68 °F) in 2.5 h and ripened more uniformly
throughout the pallet than those room-cooled.
583
ripening and other associated metabolic processes in the soil. Areas showing blotchy ripening have
(Abeles et al. 1992). For commercial ripening, less organic acids, SSC, and starch (Moretti et al.
green tomatoes should be held at 20 to 21 °C (50 2000).
to 52 °F) with 90% RH and 50 µL L-1 ethylene;
this will promote uniform ripening. Upon reaching Sunburn is associated with excessive exposure
breaker stage, tomatoes produce sufficient to sunlight and the resultant elevated tissue
ethylene and no longer require gassing. Highest temperature during fruit development, disrupting
quality tomatoes are those reaching the breaker lycopene synthesis and resulting in the appearance
stage within 3 days of ethylene exposure. These of yellow areas that remain during ripening.
fruit were harvested at the mature green stage
and will ripen with quality similar to tomatoes Blossom-end rot involves a calcium deficiency
harvested at the breaker stage or later (Maul et al. caused by either poor uptake or poor translocation
1998). into the fruit. Symptoms begin in the green fruit
as a small discoloration at the blossom end that
increases in size and becomes dry and dark brown.
Respiration Rates Occurrence increases dramatically when calcium
levels in the soil system drop below 0.08%
Tomatoes are climacteric and show a pronounced (Moretti et al. 2000). Eventually, secondary decay
increase in respiration during ripening. The organisms colonize weakened tissues.
intensity and duration of the climacteric varies
among cultivars (Wills et al. 1998). Respiration Graywall is noticeable as necrotic vascular tissue
also varies with temperature and atmospheric in the pericarp fruit wall (Jones et al. 1999). It
composition. begins developing at the green stage and has been
associated with marginal growing conditions such
Temperature Air* 3% O2 /97% N2† as cool weather, low light levels, poor nutrition,
mg CO2 kg-1 h-1 saturated soils, tobacco mosaic virus, and bacteria;
10 °C 13 to 16 6 however, the cause is still undetermined. Graywall
15 °C 16 to 28 - can be a serious disorder in both field and
20 °C 28 to 41 12 greenhouse production systems.
25 °C 35 to 51 -
*Data from Scholz et al. (1963). Storage at 10 Irregular ripening is characterized by the
°C (50 °F) is only recommended for red-ripe appearance of nonuniform ripening and white
tomatoes. internal tissue. It has been associated with feeding
†Data from Robinson et al. (1975). of sweetpotato whitefly (Bemisia argentifolii) on
tomato fruit (Hanif-Khan et al. 1997).
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and Internal bruising is recognized by the appearance
1.8 at 20 °C (68 °F). To calculate heat production, of yellow to green locular gel in ripe tomatoes.
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 It is caused by an impairment of normal ripening
or by 61 to get kcal tonne-1 day-1. of the locular gel following a physical impact at
the green or breaker stage of ripeness (MacLeod
et al. 1976). Fruit with internal bruising show
Physiological Disorders significant reductions in vitamin C content, TA,
consistency, and total carotenoids (Moretti et al.
Blotchy ripening is characterized by randomized 1998). Besides altering quality attributes, internal
development of green or green-yellowish areas bruising also affects flavor (Moretti et al. 2002).
on the surface of red tomato fruit. Apparently Breaker-stage tomatoes are more sensitive to
the development of this disorder is related to the internal bruising than those handled at the green
availability of potassium and inorganic nitrogen stage (Sargent et al. 1992).
584
Postharvest Pathology Vapor heat and hot water treatments are effective
alternatives to treatment with methyl bromide.
Tomatoes are susceptible to numerous fruit decays
from the field through postharvest handling.
Postharvest decay often develops in wounds and Suitability as Fresh-Cut Product
bruised tissue and during fruit softening. Sound
tomatoes can be inoculated by plant pathogens Despite efforts to commercialize fresh-cut
via cross-contamination from diseased fruit, dirty tomatoes, such products are still only available
harvest containers, and poorly sanitized water in limited quantities to the food-service industry,
handling systems and packing line components. particularly fast-food restaurants and catering
Populations of decay pathogens can be adequately services. After processing, loss of the gel-like
controlled through a regular sanitation program locule tissue, desiccation, water-soaking, and the
in the field and during handling, packing, and development of decay are the principal constraints
ripening-storage operations. challenging the worldwide fresh-cut industry.
Crossing commercial varieties with mutants that
Causes of bacterial decay include soft rot (Bacillus express delayed softening and slicing less ripe
spp., Erwinia carotovora ssp., Pseudomonas spp., tomatoes (in the breaker stage, for instance) for
and Xanthomonas campestris) and lactic acid subsequent application of ethylene are strategies
decay (bacterial sour rot) (Lactobacillus spp. and being researched in different parts of the world
Leuconostoc mesenteroides) (Bartz et al. 1995, to obtain a fresh-cut tomato with sufficient
Conn et al. 1995). postharvest life to be readily commercialized.
Mature-green sliced tomatoes ripen normally at 20
Fungal decay sources include alternaria rot (black °C (68 °F) (Mencarelli and Saltveit 1988). Sliced
rot) (Alternaria alternata), fusarium rot (Fusarium red tomatoes maintain good quality for 14 days
spp.), gray mold rot (Botrytis cinerea), mucor when stored in MAP at 5 °C (Hong and Gross
rot (Mucor mucedo), phoma rot (Phoma ssp.), 2001).
phomopsis rot (Diaporthe spp.), phytophthora rot
(buckeye rot) (Phytophthora spp.), pleospora rot
(Pleospora herbarum, Stemphylium botryosum Special Considerations
imp. stage), rhizopus rot (Rhizopus stolonifer,
R. oryzae), ring rot (Myrothecium roridum), Ethylene used to ripen tomatoes can be
sclerotium rot (Sclerotium rolfsii), sour rot catalytically generated from ethanol using
(Geotrichum candidum), target spot (Corynespora commercially available units or supplied from
cassiicola), and watery soft rot (Sclerotinia compressed cylinders. Because air mixtures of
minor, S. sclerotiorum). Tomato spotted wilt virus 3 to 32% ethylene are explosive (Abeles et al.
induces a mottled coloration at red stage. 1992), ethylene for ripening rooms is supplied
(Information adapted from Jones et al. [1991] and from a compressed cylinder containing a <3%
Snowdon [1992].) ethylene in N2. A metered flow of ethylene from
either a catalytic unit or compressed cylinder is
used to produce a diluted, active concentration of
Quarantine Issues ethylene in the ripening room.
585
References Hanif-Khan, S., R.C. Bullock, P.J. Stoffella, et al.
1997. Possible involvement of altered gibberellin
Abeles, F.B., P.W. Morgan, and M.E. Saltveit. metabolism in the induction of tomato irregular
1992. Ethylene in Plant Biology, 2nd ed. Acad. ripening in dwarf cherry tomato by silverleaf
Press, Waltham, MA. whitefly. J. Plant Growth Reg. 16(4):245-251.
AMS [USDA Agricultural Marketing Service]. Hong, J.H., and K.C. Gross. 2001. Maintaining
2007. United States standards for grades of quality of fresh-cut tomato slices through modified
tomatoes on the vine. At http://www.ams.usda. atmosphere packaging and low temperature
gov/AMSv1.0/getfile?dDocName=STELPR storage. J. Food Sci. 66(7):960-965.
DC5065392
Jones, J.B., J. Jones, R. Stall, and T. Zitter. 1991.
AMS [USDA Agricultural Marketing Service]. Compendium of Tomato Diseases. APS, St. Paul,
1991. United States standards for grades of fresh MN.
tomatoes. At http://www.ams.usda.gov/standards/
tomatfrh.pdf. Kader, A.A. 1986. Biochemical and physiological
basis for effects of controlled and modified
Bartz, J.A., N.C. Hodge, Y. Perez, and D. atmospheres on fruits and vegetables. Food
Concelmo. 1995. Two different bacteria cause Technol. 40(5):99-104.
a decay in tomatoes that is similar to sour rot.
Phytopathology 85:1123. Kader, A.A., and L.L. Morris. 1976. Correlating
subjective and objective measurements of
Brecht, J.K. 1994. Vegetables. In R.E. Paull and maturation and ripeness of tomatoes. In
J.W. Armstrong, eds., Insect Pests and Fresh Proceedings of the 2nd Tomato Quality Workshop.
Horticultural Products: Treatments and Responses, Vegetable Crop Series no. 178, University of
pp. 309-328. CAB International, Wallingford, California, Davis, CA.
U.K.
MacLeod, R.F., A.A. Kader, and L.L. Morris.
Brecht, J.K. 1996. Tomato response to ethylene 1976. Stimulation of ethylene and CO2 production
at high temperatures: are pressure ripening of mature-green tomatoes by impact bruising.
systems worth considering? In C.S. Vavrina, ed., HortScience 11(6):604-606.
Proceedings of the Florida Tomato Institute, pp.
11-13. Pub. no. PRO 108, University of Florida, Maul, F., S.A. Sargent, M.O. Balaban, et al. 1998.
Institute of Food and Agricultural Science, Aroma volatile profiles from ripe tomato fruit are
Gainesville, FL. influenced by physiological maturity at harvest:
an application for electronic nose technology. J.
Conn, K.E., J.M. Ogawa, B.T. Manji, and J.E. Amer. Soc. Hort. Sci. 123(6):1094-1101.
Adaskaveg. 1995. Leuconostoc mesenteroides
subsp. mesenteroides, the first report of a coccoid Maul, F., S.A. Sargent, C.A. Sims, et al. 2000.
bacterium causing a plant disease. Phytopathology Recommended commercial storage temperatures
85:593-599. affect tomato flavor and aroma quality. J. Food
Sci. 65(7):1228-1237.
Goodenough, P.W., and T.H. Thomas. 1980.
Comparative physiology of field grown tomatoes Mencarelli, F., and M.E. Saltveit. 1988. Ripening
during ripening on the plant or retard ripening in of mature-green tomato fruit slices. J. Amer. Soc.
controlled atmospheres. Ann. Appl. Biol. 94:445- Hort. Sci. 113(5):742-745.
455.
586
Moretti, C.L., E.A. Baldwin, S.A. Sargent, Sargent, S.A., and J.J. VanSickle. 1996. Premium-
and D.J. Huber. 2002. Internal bruising alters quality tomato program for Florida. In C.S.
aroma volatile profiles in tomato fruit tissues. Vavrina, ed., Proceedings of the Florida Tomato
HortScience 37(2):378-382. Institute, pp. 8-9. Pub. no. PRO 108, University of
Florida, Institute of Food & Agricultural Science,
Moretti, C.L., A.G. Calbo, and G.P. Henz. 2000. Gainesville, FL.
Postharvest physiology and handling. In J.B.C.
Silva and L.B. Giordano, eds., Processing Scholz, E.W, H.B. Johnson, and W.R. Buford.
Tomatoes, p.136-143. Embrapa/SCT, Brasilia, 1963. Heat-evolution rates of some [Lower Rio
Brazil. Grande Valley] Texas-grown fruits and vegetables.
J. Rio Grande Valley Hort. Soc. 17:170-175.
Moretti, C.L., S.A. Sargent, D.J. Huber, et
al. 1998. Chemical composition and physical Snowdon, A.L. 1992. Color Atlas of Postharvest
properties of pericarp, locule and placental tissues Diseases and Disorders of Fruits and Vegetables,
of tomatoes with internal bruising. J. Amer. Soc. vol. 2, Vegetables. CRC Press, Boca Raton FL.
Hort. Sci. 123(4):656-660.
Wills, R.B.H., B. McGlasson, D. Graham, and D.
Moretti, C.L., S.A. Sargent, D.J. Huber, et Joyce. 1998. Postharvest: An Introduction to the
al. 1999. Delayed ripening does not alleviate Physiology and Handling of Fruits, Vegetables
symptoms of internal bruising in tomato fruit. and Ornamentals. University of New South Wales
Proc. Fla. State Hort. Soc. 112:169-171. Press, Sydney, Australia.
587
Truffle Precooling conditions
Truffles are not chilling sensitive and should be There are no known quarantine issues.
stored as cold as possible without freezing.
Postharvest Pathology
589
Turnip and soft rot. The roots should have a minimum
diameter of 4.4 cm (1.75 in). Turnips are sold
bunched with tops not less than 15 cm (6 in)
Penelope Perkins-Veazie
long, short-trimmed roots with tops not more than
10 cm (4 in) long, and topped turnips with tops
Perkins-Veazie is formerly with the South Central
removed to not more than 2 cm (0.75 in). Some
Agricultural Laboratory, Agricultural Research
roots are waxed, but most are packaged as fresh
Service, U.S. Department of Agriculture,
roots and leaves or topped, and packed in vented
Lane, OK. She is now with the Department
plastic film or mesh 11.4- or 22.7-kg (25- or 50-lb)
of Horticultural Science, North Carolina State
bags or 0.45-kg (1-lb) bags packed 12 per carton.
University, NC Campus, Kannapolis, NC.
Precooling Conditions
Scientific Name and Introduction
Turnips can be cooled in the wash water, but a
Turnip (Brassica campestris, Rapaceum group) is
temperature differential of 10 °C (50 °F) or more
often confused with rutabaga or swede (B. napus,
should be avoided to prevent cracking.
Napobrassica group). Turnips have a small, white-
fleshed root, often with the surface of the top half
purple. Leaves are hairy. Roots can be grown in
Optimum Storage Conditions
warm soils, >25 °C (>77 °F) (Peirce 1987). (In
contrast, rutabagas have large, yellow-fleshed
Turnips can be held 4 to 5 mo at 0 °C (32 °F) with
roots and large, smooth leaves, and they grow in
90 to 95% RH.
cool soils.) There are also yellow-fleshed turnips.
Common turnip varieties are ‘Purple Top,’ ‘White
Globe,’ ‘White Egg,’ and ‘Golden Ball.’
Controlled Atmosphere (CA)
Considerations
Quality Characteristics and Criteria
Unknown.
High-quality turnips are firm and are free of
growth cracks, woodiness, rot, injury, and
Retail Outlet Display Conditions
pithiness.
Turnips should be stored and displayed under high
RH and refrigerated to slow shrivel. They can be
Horticultural Maturity Indices
misted.
Root diameter and freedom from woodiness are
maturity indices for turnips. If sold as topped
Chilling Sensitivity
turnips, roots should be at least 4.4 cm (1.75 in) in
diameter.
Turnips are not sensitive to chilling and should be
stored as cold as possible without freezing.
Grades, Sizes, and Packaging
Ethylene Production and Sensitivity
Grades of U.S. No. 1 and U.S. No. 2 are based
primarily on external appearance, size, and
Turnips produce no detectable ethylene and are
defects. U.S. Grade 1 roots are well trimmed, firm,
insensitive to ethylene.
fairly smooth and clean, and free from injury,
growth cracks, woodiness, water core, dry rot,
590
Respiration Rates Special Considerations
Turnips can develop growth cracks from Perkins-Veazie, P., and J.K. Collins. 1991. Color
overmaturity or boron deficiency, brown heart changes in waxed turnips during storage. J. Food
from boron deficiency, and pithiness from water Qual. 14:313-319.
stress (Snowdon 1992). Root shriveling and loss
of firmness can occur from storage at >2 °C (36 Scholz, E.W., H.B. Johnson, and W.R. Buford.
°F) or at low RH. 1963. Heat evolution rates of some Texas-grown
fruits and vegetables. J. Rio Grande Valley Hort.
Soc. 17:170-175.
Postharvest Pathology
Smith, W.H. 1957. The production of carbon
Dry rot or phoma rot (Leptospharia maculans), dioxide and metabolic heat by horticultural
watery soft rot (Sclerotina minor or S. produce. Modern Refrig. 60:493-496.
scerotiorum), alternaria rot (Alternaria brassicae),
rhizoctonia rot (Thanatephorus cucumeris), gray Snowdon, A.L. 1992. Diseases of swedes
mold (Botrytis cinerea), and bacterial soft rot (rutabagas) and turnips. In A.L. Snowdon, Color
(Erwinia crotovora ssp. carotovora) can occur Atlas of Postharvest Diseases and Disorders of
in harvested roots, generally resulting from field Fruits and Vegetables, vol. 2, Vegetables, pp. 339-
infection. 343. CRC Press, Boca Raton, FL.
Quarantine Issues
591
Waterchestnut Grades, Sizes, and Packaging
592
Retail Outlet Display Considerations Postharvest Pathology
594
Water Convolvulus Grades, Sizes, and Packaging
Ching Cheng Chen and Robert E. Paull There are no U.S. or international grades. Plants
are sold in 0.45- to 0.9-kg (1- to 2-lb) bunches
Chen and Paull are with the Department of packed in fiberboard cartons holding 9 to 18 kg
Tropical Plant and Soil Sciences, University of (20 to 40 lb).
Hawaii at Manoa, Honolulu, HI.
Precooling Conditions
Scientific Name and Introduction
Room-cooling is normally used. Vacuum-cooling
Water convolvulus (Ipomoea aquatica Forsk.) is is possible.
also called ung choi and kang kong. It is a member
of the morning-glory family (Convolvulaceae) and
is in the same genus as sweetpotato. It is thought Optimum Storage Conditions
to be native of India and is now widely consumed
as a vegetable throughout Southeast Asia. Young Tentative data suggest a storage life of 10 to 12
stems and leaves are consumed after frying days at 12 to 14 °C (54 to 57 °F) with 90 to 95%
or boiling. It has high protein and carotenoid RH.
contents. It is grown both in ponds and in moist
soil. Common varieties include types with large
or small leaves, green or greenish-yellow leaves, Controlled Atmosphere (CA)
and stems that are white or green. There are many Considerations
common names besides the three given above:
kong xin cai, water spinach, water cabbage, and Tissue browning is promoted by 3% CO2 at
pake boong. 1 °C (33 °F) but is prevented by higher CO2
concentrations at 20 °C (68 °F) (Ose et al. 1999).
Water convolvulus produces about 2 µL kg-1 Austin, D.F. 1991. Associations between the plant
h-1 ethylene at harvest; the rate declines during family Convolvulaceae and cylas weevils. In R.K.
storage. Production can then increase to about Jansson and K.V. Raman, eds., Sweet Potato Pest
the same level when leaves senesce and turn Management: A Global Perspective, pp. 45-78.
yellow. Ethylene exposure induces premature leaf Westview, Boulder, CO.
senescence and yellowing.
Cornelis, J., J.A. Nugteren, and E. Westphal.
1985. Kang kong (Ipomoea aquatica Forsk.): an
Respiration Rates important leaf vegetable in South-East Asia. Abst.
Trop. Agric. 10:9-21.
Respiration rate is 50 to 150 mg (28 to 85 µL)
CO2 kg-1 h-1 at 27 °C (81 °F). Heat production is Ho, B.W.C., and H.H. Edie. 1969. White rust
11,000 to 33,000 BTU ton-1 day-1 or 3,050 to 9,150 (Albergo ipomoeae-aquatica) on Ipomoea
kcal tonne-1 day-1. aquatica in Hong Kong. Plant Dis. Rep. 53:959-
962.
Quarantine Issues
596
Watercress Optimum Storage Conditions
Stephen Morris and Jenny Jobling Watercress can be stored for 2 to 3 weeks at 0
°C (32 °F) with >95% RH (Hruschka and Wang
Morris and Jobling are with the Sydney 1979). Shelf-life is reduced to 2 to 3 days if stored
Postharvest Laboratory, Food Science Australia, under low RH.
North Ryde, New South Wales, Australia.
Controlled Atmosphere (CA)
Specific Name and Introduction Considerations
Watercress (Nasturtium officinale R.Br.) has been The rate of yellowing can be reduced by storing in
known in Europe and Asia for thousands of years, atmospheres >7% CO2 with not less than 5% O2
and it is now grown in many countries worldwide. (Aharoni et al. 1989).
It is a member of the Brassicaceae (Cruciferae)
family and is used as a leafy salad vegetable. It
has attractive dark green leaves, a strong flavor Retail Outlet Display Considerations
and is rich in vitamins. Watercress is typically
grown in running water (Snowdon 1991). Contact with melting ice and water sprays help
in preventing dehydration. Careful handling
is necessary to avoid crushing or bruising the
Horticultural Maturity Indices delicate leaves.
Quarantine Issues
Special Considerations
References
598
Watermelon reliable method of determining maturity within
a given field is to visually examine the fruit for
the changes described above, then cut some fruit
James W. Rushing
in random sectors of the field to check flavor and
internal color development. Some buyers require
Rushing is formerly with the Coastal Research
that fruit have some minimum SSC, which is
and Education Center, Clemson University,
easily measured with a refractometer (Sackett
Charleston, SC. He is now retired.
1974, Rushing et al. 2000).
Watermelons destined for export must be free Hardenburg, R.E., A.E. Watada, and C.Y.
of disease, insects, soil, or vegetative debris. Wang. 1986. The Commercial Storage of Fruits,
Phytosanitary requirements in the receiving Vegetables, and Florist and Nursery Stocks,
country should be reviewed prior to shipment. pp. 12,62. Agricultural Handbook 66, U.S.
Department of Agriculture, Washington, DC.
Biglete, N.A. 1992. Packaging And Storage Suslow, T.V. 1999. Watermelon.
of Watermelon Using Different Shrink-Wrap Recommendations for maintaining postharvest
Techniques. M.S. thesis, Clemson University, quality. At http://postharvest.ucdavis.edu/produce_
Clemson, SC. information.
Robert E. Paull and Ching Cheng Chen Room-cooling is normally used because of the risk
of excessive moisture loss with forced-air cooling.
Paull and Chen are with the Department of
Tropical Plant and Soil Sciences, University of
Optimum Storage Conditions
Hawaii at Manoa, Honolulu, HI.
Storage at 2 to 10 °C (36 to 50 °F) is
recommended. However, chilling injury is a
Scientific Name and Introduction
problem at these temperatures. A conservative
recommendation would be 12 to 14 °C (54 to 57
Wax apple (Syzygium samarangense [Blume]
°F) with 90 to 95% RH, which should result in a
Merrill & L. M. Perry) is the main species in this
shelf-life of 10 to 14 days.
Southeast Asian genus that is consumed fresh.
Other species with similar fruits are S. aqueum,
rose water apple; S. aimini, java plum; S. jambos,
Controlled Atmospheres (CA)
rose apple; and S. malaccense, Malay apple. The
Consideration
spice clove is S. aromaticum.
No CA studies have been reported. MAP in sealed
The fruit is broad, bell-shaped or sometimes oval,
polyethylene film bags reduces chilling injury
5 to 6 cm (2 to 2.5 in) long, and 4 to 5 cm (1.5 to
and decay (Horng and Peng 1983). Waxing is less
2 in) wide. It has one to four seeds. The skin can
effective, partly due to RH control.
be green to light red to dark red and has a waxlike
high gloss sheen. The low-acid flesh is white and
juicy (Nakasone and Paull 1998).
Retail Outlet Display Considerations
Respiration declines after harvest (Liao et al. Akamine, E.K., and T. Goo. 1979. Respiration
1983). and ethylene production of fruits of species and
cultivars of Psidium and species of Eugenia. J.
Temperature mg CO2 kg-1 h-1 Amer. Soc. Hort. Sci. 104:632-635.
10 °C 4 to 5
20 °C 8 to 11 Horng, D., and C. Peng. 1983. Studies on package,
transportation and storage of wax apple fruits
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate (Syzygium samarangense). Natl. Chung Hsing
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and Univ. Hort. J. 8:31-39.
1.8 at 20 °C (68 °F). To calculate heat production,
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 Liao, M.L., M.S. Liu, and J.S. Yang. 1983.
or by 61 to get kcal tonne-1 day-1. Respiration measurement of some important fruits
in Taiwan. Acta Hort. 138:227-246.
Postharvest Pathology
Quarantine Issues
603
Fresh-Cut Fruits and flavor while still maintaining freshness.
Several commodities, although botanically fruits
John C. Beaulieu and James R. Gorny (for example, cucumber, pepper, and tomato),
will not be covered in this section since they are
Beaulieu is with the Southern Regional Research commonly classified as vegetables.
Center, Agricultural Research Service, U.S.
Department of Agriculture, New Orleans, LA.
Gorny is formerly with the International Fresh-cut Fresh-Cut Physiology and Physiological
Produce Association, Alexandria, VA. He is now Concerns
with the Produce Marketing Association, Newark,
DE. Once harvested, fruits are removed from their
source of water, minerals, and sustenance. Fruit
tissues continue to respire, using available and
Introduction and Overview stored sugars and organic acids, and they begin
to senesce rapidly. Postharvest quality loss is
Fresh-cut fruit products for both retail and food primarily a function of respiration, onset or
service applications have enjoyed an increasing progression of ripening (climacteric fruit), water
presence in the marketplace due to demand by the loss (transpiration), enzymatic discoloration of
consumer. In the coming years, it is commonly cut surfaces, decay (microbial), senescence, and
perceived that the fresh-cut fruit industry will mechanical damage suffered during preparation,
have unprecedented growth. For this reason, shipping, handling, and processing (Schlimme
many leading fresh-cut salad manufacturers have and Rooney 1994, Watada et al. 1996). Fruits
targeted development of fresh-cut fruit products as destined for fresh-cut processors should be
part of their long-term business plans. However, harvested as ripe as possible. This makes it critical
processors of fresh-cut fruit products face that temperature-dependent events related to
numerous challenges not commonly encountered respiration, water loss, pathological decay, and
during fresh-cut vegetable processing. The ethylene production be strictly regulated during
difficulties encountered with fresh-cut fruit, while shipment (or storage) of the fruit.
not insurmountable, require a new and higher
level of technical and operational sophistication. During the climacteric ripening stage of many
fruits, there is a dramatic increase in respiratory
production of CO2 and ethylene. Non-climacteric
Fresh-Cut Defined for Fruits fruit, leafy vegetables, non-fruit vegetables, and
roots and tubers do not have a surge in ethylene
The USDA and FDA definitions for “fresh” and production and generally have only slightly
“minimally processed” fruits and vegetables increased respiration as senescence approaches.
imply that fresh-cut (precut) products have been However, if severely wounded (for example,
freshly cut, washed, packaged, and maintained by fresh-cut processing), significant stress-
with refrigeration. Fresh-cut products are raw, induced production of CO2, and often ethylene,
and even though processed (physically altered occurs (Abeles et al. 1992, Brecht 1995). Fresh-
from the original form), they remain in a fresh cut processing increases respiration rates and
state, ready to eat or cook, without freezing, causes major tissue disruption as enzymes and
thermal processing, or treatments with additives substrates, normally sequestered within the
or preservatives (AMS 1998, Anonymous 1998). vacuole, become mixed with other cytoplasmic
The United Fresh Produce Association defines a and nucleic substrates and enzymes. Processing
fresh-cut product as fruits or vegetables that have also increases wound-induced ethylene, water
been trimmed and/or peeled and/or cut into 100% activity, and surface area per unit volume, which
usable product that is bagged or prepackaged may accelerate water loss and enhance microbial
to offer consumers high nutrition, convenience, growth since sugars also become readily available
604
(King and Bolin 1989, Watada et al. 1990, Wiley to a processor and rejected because the fruit are
1994, Watada and Qi 1999). too soft or ripe for cutting. Determination of
optimum maturity depends on the commodity
These physiological changes may be accompanied and the use. The processor must understand the
by flavor loss, cut surface discoloration, color physiology of the fruit and the finished product
loss, decay, increased rate of vitamin loss, rapid (and packaging) to accurately determine when
softening, shrinkage, and a shorter storage fruit is at the appropriate maturity stage to process.
life. Increased water activity and mixing of Choice of variety, harvest condition, maturity,
intracellular and intercellular enzymes and storage, and shelf-life for various fresh-cut fruits
substrates may also contribute to flavor and are active areas of research.
texture loss during and after processing.
Therefore, proper temperature management during Proper initial maturity of fruit is essential.
product preparation and refrigeration throughout However, once processed, quality is most
distribution and marketing are essential for commonly, and sometimes only, assessed visually.
maintenance of quality. Visual appearance is generally the determinant for
commercial shelf-life. Although some studies have
Physical alterations and potential low-O2 found that vitamin C and carotene degrade very
atmospheres in packages may create significant little during short-term (about 1 week) refrigerated
negative changes in flavor, aroma, and “mouth- storage in some fresh-cut fruits (Wright and Kader
feel.” There are also synergistic interactions 1997a,b), other researchers are attempting to retain
between numerous factors such as variety, source, fresh-cut quality for 3 to 4 weeks. Although some
season, initial maturity, optimum processing quality attributes may still be acceptable, overall
maturity, slicing and cutting equipment, sanitation quality in terms of aroma, taste, and texture may
and GRAS chemical treatments, packaging be jeopardized (Anonymous 2000a).
(including MAP), temperature management,
shipping, handling, and length of shelf-life.
The combined effect of these factors may have Firmness and Texture
negative consequences on postharvest shelf-
life and sensory quality. Therefore, improperly Tissue softening is a very serious problem with
preparing, packaging, and handling fresh-cut fruit fresh-cut fruit products and can limit shelf-life.
may compromise overall quality and decrease Fresh-cut fruit firmness is an important quality
consumer acceptability. attribute that can be affected by cell softening
enzymes present in the fruit tissue (Varoquaux et
al. 1990) and by decreased turgor due to water
Defining Fresh-Cut Fruit Quality loss. For example, there was 26 to 49% firmness
loss in four varieties of fresh-cut cantaloupe
Postharvest quality and post-cutting quality are processed from three-quarters to full-slip maturity
unfortunately ambiguous or confused terms. fruit when stored in air at 4 °C (39 °F) (Beaulieu
Harvest indices used to deliver optimum quality 2002, unpublished data). Unwrapped watermelon
whole fruits to storage facilities, terminal markets, slices lost 47% of their firmness after 4 days at
and the fresh markets oftentimes may not be 5 °C (41 °F) (Abbey et al. 1988), and firmness
appropriate for fruits destined to be processed decreased in stored cantaloupe cubes (12 days
(see also Factors Affecting Fresh-Cut Fruit in air at 5 °C) by more than 25% (Cantwell and
Quality). Growers often destructively sample fruit Portela 1997).
before making a decision to harvest. Processor
assessment of fruit maturity before cutting either Flesh firmness of fresh-cut fruit products can
upon receipt or after in-house storage is also be maintained by application or treatment with
advisable. For example, a lot of peaches that are calcium compounds. Dipping fresh-cut products in
optimally mature for fresh market may be shipped solutions of 0.5 to 1.0% calcium chloride is very
605
effective in maintaining product firmness (Ponting cause anaerobic metabolism and production of off
et al. 1971, 1972). However, calcium chloride may flavors and odors.
leave bitter off flavors on some products. Firmness
of slices from 12 untreated apple cultivars stored
at 2 °C (36 °F) decreased steadily for 7 days Acidification
and more rapidly thereafter (Kim et al. 1993).
However, mild heat treatment of whole apples PPO most effectively catalyzes cut surface
before processing maintained firmness during discoloration at a neutral pH of approximately 7.
storage in some fresh-cut apple cultivars (Kim et Therefore, browning can be slowed by dipping
al. 1994). products in mildly acidic food grade solutions
of acetic, ascorbic, citric, tartaric, fumaric, or
Firmness can sometimes be maintained by CA phosphoric acid. However, these acids may leave
storage. Firmness loss averaged 2.2 lb (10 N) in off flavors and promote tissue softening and
honeydew cylinders after 12 days of storage in therefore must be used with care.
air at 5 °C (41 °F), while CA storage (air + 15%
CO2) reduced the loss significantly in one of four
cultivars tested (Portela and Cantwell 1998). CA Reducing Agents
treatments (2% O2 + 10% CO2 at 5 °C [41 °F]
and 4 % O2 + 10% CO2 at 10 °C [50 °F]) were Ascorbic acid or erythorbate (an isomer of
more beneficial than air storage in maintaining ascorbic acid) are two common compounds used
honeydew cube quality for up to 6 days at 5 °C in the food industry to prevent PPO-mediated
(41 °F) (Qi et al. 1999). cut-surface discoloration. Ascorbic acid and
erythorbate reduce PPO-induced discoloration at
the cut surface by converting quinones (formed
Color at the Cut Surface by PPO from phenolics) back to phenolic
compounds. Unfortunately, once all the ascorbic
An important issue in fresh-cut fruit processing is acid or erythorbate is exhausted, PPO browning
the control of discoloration (pinking, reddening, will proceed uninhibited. Ascorbic acid or
or blackening) or browning on cut surfaces. erythorbate are commonly used as 1% solutions
Oxidative browning is usually caused by the to prevent browning and discoloration of cut
enzyme polyphenol oxidase (PPO), which in the surfaces. These compounds are organic acids, so
presence of O2 converts phenolic compounds in they may also reduce surface pH of commodities,
fruits and vegetables into dark-colored pigments. further slowing browning.
Outlined below are a number of strategies that
may be used to reduce PPO-mediated cut-surface
discoloration. Sensory Aspects
606
flavor and texture may enhance consumers desire by day 8; pH tended to increase through storage
to repeatedly purchase such products. (except when stored under 2% O2) (Wright and
Kader 1997b). In cantaloupe slices, a 17% loss
in SSC and a 2-fold increase in TA occurred
Soluble Solids Content (SSC) and Titratable after only 2 days storage at 20 °C (68 °F), but
Acidity (TA) the acidity change was attributed to lactic acid
bacteria (Lamikanra et al. 2000). TA in fresh-cut
Sweetness, flesh firmness, and taste are very oranges stored 8 days at 4 °C (39 °F) decreased
important characteristics for fresh-cut melon 36% (Rocha et al. 1995).
quality. In a midseason trial of 17 western
cantaloupe varieties, there was an average 5%
decrease in SSC (range 0 to 11%) and an average Aroma and Flavor
8% decrease in sugar (range 0 to 21%) when
cubes were stored 12 days (in air) at 5 °C (41 An acceptable post-cutting visual appraisal
°F) (Cantwell and Portela 1997). After 9 days at does not necessarily imply that a product has
10 °C (50 °F) or 15 days at 5 °C (41 °F), SSC satisfactory flavor quality. Excellent visual quality
in CA-stored melon pieces were higher than in and acceptance by retailers and consumers often
air: 10.3 vs. 9.5% and 10.2 vs. 9.1% at 10 and occur with fruits processed when immature. For
5 °C (50 and 41 °F), respectively (Cantwell and example, immature peaches and nectarines will
Portela 1997). Cantaloupe balls prepared from process and hold visual quality for extended
four eastern varieties stored 8 days at 0 °C (32 periods, but rehardening and poor eating-quality
°F) had an average SSC decrease of 9.7% with a limit their use (Gorny et al. 1998b, Beaulieu
range of 2.3 to 13% (Lange 1998). SSC remained et al. 1999). A mature green cantaloupe at less
somewhat constant for 7 days storage at 4 °C (39 than half-slip delivers a fresh-cut product with
°F) in fresh-cut cantaloupe when harvest maturity optimum visual shelf-life but insufficient sugar or
was at least half-slip, and cubes prepared from volatile composition compared to a desirable ripe
fruit harvested at quarter-slip had significantly whole melon (Pratt 1971, Beaulieu and Grimm
lower initial SSC, which rapidly declined after 2001, Beaulieu and Baldwin 2002). ‘Makdimon’
only 5 days storage (Beaulieu and Baldwin melons harvested 2 days before fully-ripe (full-
2002). It is well established in the food industry slip) developed only about 25% the total volatiles
that sugar content (SSC) is generally positively as 3 day-old, fully-ripe fruit (Wyllie et al. 1996).
correlated with desirable flavor quality. However, Volatiles increased with harvest maturity, and
occasionally too much sugar is perceived cubes prepared from quarter-slip fruit contained
negatively. The best sugar range for storage of only 25 to 33% of the total volatiles of full-slip
fresh-cut cantaloupe was 10 to 13 °Brix. However, fruit (Beaulieu and Baldwin 2002). Furthermore,
some judged the 13 °Brix fruit as too sweet these trends were conserved during 10 days at 4°C
(Anonymous 2000b). (39 °F) in fresh-cut products.
TA and SSC have also been used to assess quality Flavor and aroma quality are important attributes
via the SSC:TA ratio in some fresh-cut fruits. for consumers, and these attributes should be
Changes in TA, pH, and SSC in apple slices from seriously examined when determining the shelf-
12 cultivars that were stored at 2 °C (36 °F) for life of fresh-cut fruit products. Nevertheless,
12 days were small and varied by cultivar (Kim the quality of intact vegetables and fruits is
et al. 1993). Likewise, there were changes in SSC often determined almost exclusively based
in fresh-cut strawberries stored under various on appearance, sometimes to the exclusion of
CA for 7 days at 5 °C (41 °F). However, pH flavor and texture (Sapers et al. 1997). Much
increased over time (Wright and Kader 1997b). variability exists in the literature regarding
Fresh-cut persimmons stored under various CA acceptability based on sensory evaluations, and
had increased SSC for 3 days, then decreased SSC this variability oftentimes can be attributed to
607
different experimental designs or sensorial analyses Microbiology
and cultural bias. For example, sensory evaluation
determined that fresh-cut honeydew, kiwi fruit, Microbial decay can be a major source of spoilage
papaya, pineapple, and cantaloupe stored at 4 of fresh-cut produce (Brackett 1994). Microbial
°C (39 °F) were unacceptable after 7, 4, 2, about decay of fresh-cut fruit may occur much more
7, and 4 days, respectively (O’Connor-Shaw rapidly than in vegetable products because of
et al. 1994). However, fruit were not sanitized, the high levels of sugars found in most fruit.
nor were gloves worn during preparation and The acidity of fruit tissue usually helps suppress
subsequent microbial decay and associated bacterial growth but not growth of yeast and
texture loss most likely limited post-cutting life. molds. There is no evidence to suggest that lower
Sterilized, diced cantaloupe stored at 4 °C (39 °F) aerobic plate counts (APC) or total plate counts
in various controlled atmospheres were acceptable (TPC) immediately after processing correlate
from a sensory quality standpoint after 28 days with increased shelf-life in fresh-cut vegetables.
(O’Connor-Shaw et al. 1996). Cantaloupe pieces However, for fresh-cut fruit, very low APC, TPC,
stored at 2 °C (36°F) in ready-to-serve tray-packs and especially yeast and mold counts correlate
were visually acceptable after 19 days, but flavor with increased shelf-life.
scores fell after 13 days storage (Silva et al. 1987).
An informal taste panel determined that fresh-cut The dominant microorganisms associated with
honeydew melon stored in air at 5 °C (41 °F) for 6 spoilage of fresh-cut vegetables are bacteria
days lacked acceptable textural characteristics and (for example, Pseudomonas spp.), whereas the
were flat in flavor (Qi et al. 1998). dominant microorganisms associated with the
spoilage of fresh-cut fruit products are yeasts and
Fresh-cut pineapple stored at 4 °C (39 °F) had molds. In fresh-cut vegetables the proliferation
excellent visual appearance after 7 to 10 days of bacteria may be a symptom associated with
storage; however, fruit in the lower portion of tissue senescence and may not be a true cause of
containers developed off flavors associated with spoilage except in a few rare exceptions when
microbial fermentation (Spanier et al. 1998). pectinolytic Pseudomonas are present. However,
Fresh-cut orange segments that had acceptable in acidic fresh-cut fruit products, yeasts and molds
appearance after 14 days storage were found to are typically associated with product spoilage.
have unacceptable flavor quality after only 5 days Reducing initial yeast and mold counts, as well as
at 4 °C (39 °F) (Rocha et al. 1995). Likewise, slowing growth by low temperature storage at <5
undesirable flavor was the limiting factor in sliced °C (41 °F), affects product shelf-life (O’Connor-
wrapped watermelon stored 7 days at 5 °C (41 Shaw et al. 1994, Qi et al. 1999). In fresh-cut fruit
°F), even though aroma was still acceptable and with a neutral pH, such as cantaloupe, bacteria
microbial populations were not problematic until were the main source of spoilage (Lamikanra
after 8 days (Abbey et al. 1988). et al. 2000), and bacterial development was
inhibited in fresh-cut watermelon by CA (3% O2
Establishing overall shelf-life limits for fresh-cut and 15 or 20% CO2). However, visual quality was
fruit, taking flavor quality into consideration, is compromised (Cartaxo et al. 1997).
difficult since initial product variability, potential
post-cutting treatments, and packaging affect flavor Little research has been performed on foodborne
attributes differently. Washing whole products prior human pathogens on fresh-cut fruits. Conway et
to processing and other proper sanitation practices, al. (2000) determined that Listeria monocytogenes
in combination with optimum storage temperature, survived and proliferated on ‘Delicious’ apple
are critical to maintaining quality and prolonging slices stored at 10 or 20 °C (50 or 68 °F) in air
product life. Little is known concerning what effect or CA (0.5% O2 + 15% CO2) but did not grow
storage temperature has on volatile production and at 5 °C (41 °F). CA had no significant effect on
little flavor and sensory work has been performed the survival or growth of L. monocytogenes at
on fresh-cut fruits. elevated temperatures. Botulinal toxin was not
608
recovered in fresh-cut cantaloupe or honeydew longer than fruit cut with a dull hand-slicer (Gorny
inoculated with a 10-strain mixture of proteolytic and Kader 1996). Sharpening of machine and
and nonproteolytic Clostridium botulinum after hand knives as often as possible prolongs shelf-
21 days at 7 °C (45 °F). However, toxin was life of fresh-cut fruit because there is less tissue
recovered in some inoculated honeydew samples injury.
stored 9 days at 15 °C (59 °F) in hermetically
sealed packages (Larson and Johnson 1999).
Chilling Injury and Holding Temperatures
Factors Affecting Fresh-Cut Fruit Quality A significant number of fresh-cut fruits are not as
chilling injury (CI) sensitive as the corresponding
Major factors affecting fresh-cut fruit quality are intact fruit before processing. Examples include
cultivar (Kim et al. 1993, Romig 1995), preharvest pineapple, cantaloupe, honeydew, watermelon,
cultural practices (Romig 1995), harvest maturity peach, nectarine, and mango. If these intact fruits
(Gorny et al. 1998b), physiological status of the are stored at chilling temperatures, typically <12
raw product (Brecht 1995), postharvest handling °C (54 °F), accelerated physiological breakdown
and cold storage (Watada et al. 1996), processing and increased incidence of pathological decay
technique (Bolin et al. 1977, Saltveit 1997, Wright occurs. CI symptoms are often manifested when
and Kader 1997b), sanitation (Hurst 1995), and fruit are subsequently placed at non-chilling
packaging (Solomos 1994, Cameron et al. 1995). temperatures and may not be visible if maintained
at chilling temperatures (Saltveit and Morris
1990). Nonetheless, precooling whole cantaloupe
General Fresh-Cut Physiology and Physiological to below their optimal long-term storage
Concerns temperature shortly before cutting is effective at
increasing product shelf-life (Cantwell and Portela
Most fruit are very susceptible to bruising and 1997, Lange 1998).
mechanical injury. This is very different from
most fresh-cut vegetables, which may be derived Fruit tissues normally damaged by storage at
from very durable root tissues (carrots, radishes) chilling temperatures are the inedible outer rind
or pliable leaf tissue (iceberg lettuce, cabbage). or skin portions. During fresh-cut processing,
Fresh-cut processing removes the fruit’s natural these tissues are normally removed and discarded.
cuticle, or skin barrier, to gas diffusion and Although the optimal storage temperature for
microbial invasion, and severe disruption of many whole CI-sensitive fruit is above 10 °C
the tissue often provokes increased respiration, (50 °F), after processing storage at 0 °C (32°F)
ethylene production, and enhanced susceptibility is almost always the temperature that provides
to water loss and microbial decay. All of these optimal shelf-life by reducing growth of spoilage
factors may contribute to decreased shelf-life via microorganisms. However, the edible flesh of CI-
browning, off color, softening, or decay. sensitive fruits may still be susceptible to chilling
injury, and no studies have indicated if flavor
Subsequently, methods for cutting and peeling biosynthesis is inhibited or negatively affected by
fruit differ from those for vegetables. Therefore, chilling.
mechanical size reduction (trimming, peeling,
deseeding, etc.) by high-speed cutting equipment
may not be appropriate for some fresh-cut fruit Variety, Growing Region, and Season
products. Knife sharpness has a significant effect
on shelf-life of fresh-cut lettuce products (Bolin Seed companies and numerous fresh-cut
et al. 1977, Bolin and Huxsoll 1991), and this also processors are already aware that a given variety
applies to fresh-cut fruits. Pear slices cut with a performs optimally in certain growing regions
freshly sharpened knife retained visual quality and oftentimes has variable postharvest quality
609
attributes depending on cultural practices, climate, each, due to severe enzymatic browning on cut
season, and harvest maturity. For example, the surfaces (Gorny et al. 1998a). However, ‘Bartlett’
desirable volatile oil content of pineapple flesh and ‘Bosc’ pear slices experienced a much greater
is higher in summer fruit (Haagen-Smit et al. loss in firmness after slicing and storage in air at
1945), and the proportions of dominant apple 10 °C (50 °F) than d‘Anjou’ and Red d’Anjou’
volatiles vary by season (López et al. 1998). slices.
The aforementioned interactions, in concert with
breeding against or for specific traits to optimize
shelf-life, must be considered when developing Fruit Size and Yield
cultivars tailored for the fresh-cut industry (Romig
1995). Several reports have documented that Typically, fresh-cut fruit processors will use
certain cultivars out-perform others with regard to either very large or very small fruit to maximize
fresh-cut shelf-life and quality (Kim et al. 1993, yields or to reduce the cost of raw ingredients. For
Cantwell and Portela 1997, Lange 1998, Gorny et example, fresh-cut melon processors will typically
al. 1999, Anonymous 2000b). However, no single use very large 9-count-per-box fruit. This is
study can encompass all desirable varieties, and because large melons are often available at lower
singling out a “winner” can be compromised by prices in the marketplace, the yield from larger
seed source and seasonal/climactic variations. melon fruits is almost always higher, and the
Furthermore, the industry may also be historically labor to process one large fruit is often less than
driven toward specific varieties (such as western processing many smaller fruit. Very little research
cantaloupes) when indeed optimum alternatives has been done to document the effects of fruit
exist for local seasonal production (such as eastern size on post-cutting shelf-life and quality. One
cantaloupes) (Lange 1998). study by Gorny et al. (2000) found that ‘Bartlett’
pear fruit size did not have a significant effect on
Gorny et al. (1999) determined the shelf-life of fresh-cut slice shelf-life, based on flesh color and
peach and nectarine slices made from 13 cultivars firmness, if slices were treated after cutting with
of peaches and eight cultivars of nectarines that 2% ascorbate + 1% calcium lactate + 0.5% (w/v)
had been ripened to between 4 to 7 lb (18 to 31 cysteine, pH 7. However, if slices were not treated,
N) firmness, cut, and then held at 0 °C (32 °F) smaller fruit discolored at their cut surface more
with 90 to 95% RH. Shelf-life was 2 to 12 days rapidly than slices from large fruit. Small fruit also
among the cultivars tested. Of the peach cultivars had lower SSC than large fruit, which may affect
tested, ‘Cal Red,’ ‘Red Cal,’ and ‘Elegant Lady’ eating quality. These findings demonstrate that,
had the longest marketable shelf-life of 7.4, 7.2, in some cases, smaller whole fruit, which often
and 6.7 days, respectively; while ‘Summer Lady’ receive lower prices in the marketplace, should be
and ‘Ryan Sun’ had the shortest (<2 days). Among avoided for value-added fresh-cut products.
nectarines, ‘Sparkling Red,’ ‘Arctic Queen,’ and
‘Zee Grand’ had the longest shelf-life of 12, 8,
and 8 days, respectively, while the other cultivars Physical Treatments
had a shelf-life of 4 to 6 days. White-flesh peaches
and nectarines had a comparable shelf-life to Many physical and chemical techniques have
yellow-fleshed cultivars, with similar browning been studied as alternatives or adjuncts to MAP,
characteristics. especially for fresh-cut fruit: edible coatings
(Baldwin et al. 1995a,b, 1996, Howard and Dewi
Based on visual quality, fresh-cut pear slices 1996, Li and Barth 1998), disinfection (Hong and
prepared from partially ripened ‘Bosc’ and Gross 1998, Sapers and Simmons 1998), natural
‘Bartlett’ fruit had the longest shelf-life in air at plant products (Kato-Noguchi and Watada 1997,
10 °C (50 °F), being 3 and 4 days, respectively Leepipattanawit et al. 1997, Buta et al. 1999,
(Gorny et al. 1998a). d‘Anjou’ and ‘Red Anjou’ Moline et al. 1999), ethylene absorbents (Abe and
pear slices had a very short shelf-life of <2 days Watada 1991), gamma irradiation (Chervin and
610
Boisseau 1994, Hagenmaier and Baker 1997), Modified Atmosphere Packaging (MAP)
heat treatments or heat shock (Loaiza-Velarde
et al. 1997), microbial competition (Liao 1989, MAP is widely used for fresh-cut vegetables, but
Breidt and Fleming 1997), non-thermal physical with fruits occasionally undesirable atmospheres
treatments (Hoover 1997), and pulsed-microwave can reduce quality due to off flavor and
irradiation (Shin and Pyun 1997). discoloration (Gil et al. 1998).
The beneficial effects of CA storage for whole Slices prepared from heat-treated apples at 45
fruit have been well documented, and CA is °C (113 °F) for 105 min, that did not display
widely employed throughout the industry. browning after treatment (‘Delicious,’ ‘Empire,’
However, CA storage markedly inhibits apple ‘Golden Delicious,’ ‘McIntosh’ and ‘New York
volatile production (Yahia 1991, 1994, Mattheis 674’), were firmer (differences ranging from 12%
et al. 1995). Furthermore, fruit maturity at harvest for ‘McIntosh’ to 48% for ‘Delicious’) after 8 days
has been shown to be important in terms of storage at 2 °C (36 °F) than those prepared from
volatile production in melons (Pratt 1971, Wyllie untreated apples (Kim et al. 1994). However, heat
et al. 1996, Beaulieu and Grimm 2001, Beaulieu treatments often led to undesirable flesh browning
and Baldwin 2002) and in apples (Hansen et al. in many other cultivars tested (Kim et al. 1994).
1992, Brackmann et al. 1993, Yahia et al. 1990),
especially once apple fruit were removed from CA
and ripened 10 days at 20 °C (68 °F) (Mattheis Irradiation
et al. 1995). In fresh-cut ‘Gala’ apples stored for
14 days at 1 °C (34 °F) in sealed pouches, sugars Irradiation of fresh-cut fruit products may be
remained constant during storage, pH decreased, beneficial in reducing the number of bacteria
and TA, sweetness, and sweet aromatic flavor all present on the product. The current FDA limit
increased and then decreased (Bett et al. 2001). for irradiation on fresh produce is 1.0 kGy, but
Therefore, certain packaged fresh-cut products to destroy yeasts and molds that may exist as
may require active modification of the atmosphere spores, irradiation levels of 1.5 to 20 kGy are
to ensure desirable flavor during consumption. necessary (Brackett 1987); and these levels are
damaging to fruit tissues. Irradiation reduced
Gorny et al. (2002) determined that, compared ethylene production of all pre- versus post-
to air storage, CA (2% O2 + 98% N2) storage climacteric apple slices and irradiation doses of up
at -1 °C (30 °F) of whole mature-green pears to 2.4 kGy had minimal effect on the respiratory
extended shelf-life of slices 1 to 2 days. There was physiology of tissues (Gunes et al. 2000).
a significant reduction in shelf-life of pear slices However, tissue softening occurred at doses
stored at -1 °C (30 °F) in air or CA compared to above 0.4 kGy. Therefore, the use of irradiation
slices from freshly harvested pears. Therefore, it to extend the shelf-life of fresh-cut fruit products
seems beneficial to use CA for off-season pears has only limited benefits since the main spoilage
(as opposed to air-stored) to maximize post- microorganisms on fresh-cut fruit products are
cutting life of slices. However, research is needed yeasts and molds.
to determine if volatile synthesis is impaired, as in
CA-stored apples.
611
Chemical Treatments GRAS status regarding use on fresh-cut products
is currently unclear.
Retention of fresh-cut product firmness and
inhibition of browning are common measures
used to determine efficacy of chemical treatments. Calcium Compounds and Firmness Retention
Calcium has been used as an agent for maintaining
firmness of whole produce (Poovaiah 1986), and Application of aqueous calcium compounds
its use in fresh-cut was inevitable. Other chemical (generally 1% CaCl2 dips) helps maintain firmness
treatments have also been explored, and most of fresh-cut apples, pears, and strawberries
GRAS applications used today involve chlorine, (Ponting et al. 1971, 1972, Morris et al. 1985,
ascorbic acid, or calcium salts for preservation. Rosen and Kader 1989, Gorny et al. 1998a).
Softening of muskmelon sections was affected
differently depending on calcium concentration
Chlorination and Washes (Lester 1996). Softening was the major factor in
quality loss in kiwifruit slices. However, these
In general, fresh-cut fruit should be rinsed just slices had a shelf-life of 9 to 12 days if treated
after cutting with cold (0 to 1°C, 32 to 34 °F) with 1% CaCl2 or 2% calcium lactate and stored
chlorinated water at pH 7.0. This may help extend at 0 to 2 °C with >90% RH in 2 to 4% O2 and/or 5
product shelf-life by reducing microbial load, to 10% CO2 (Massantini and Kader 1995, Agar et
removing cellular juices at cut surfaces that may al. 1999). Cantaloupe cylinders treated with 1, 2.5,
promote cut surface discoloration, and actually and 5% CaCl2 for 1 to 5 min and stored 10 days
inhibiting the enzymatic reactions involved in fruit at 5 °C (41 °F) generally increased in firmness
browning (Brecht et al. 1993, Hurst 1995). (Luna-Guzmán et al. 1999).
Chlorination, as commonly used for fresh-cut Calcium lactate has been shown to be as effective
salad sanitation (not exceeding 200 µL L-1 total as the chloride form without imparting a bitter
chlorine), may not be desirable for all fresh- flavor at higher concentrations (Luna-Guzmán
cut fruits. Post-cutting washing or dipping may and Barrett 2000). A 1% calcium lactate dip was
have negative consequences regarding increased an effective alternative to ascorbate in fresh-cut
water activity and “washing away” of desirable ‘Bartlett’ pears stored 1 to 2 days at 20 °C (68 °F),
flavor attributes. Processors may or may not and 1% calcium lactate with 2% ascorbate was
wash freshly cut commodities that develop little most effective (Gorny et al. 1998a).
or no browning (cantaloupe and honeydew, for
example), since chemical treatments are seldom
applied and because removal of free surface water Antibrowning Compounds
(centrifugation or spinning) from the cut fruit can
be damaging. On the other hand, honeydew and Many fruits brown rapidly after cutting, and
cantaloupe pieces dipped in hypochlorite (pH 6, extensive work has been performed to address
50 µL L-1) prior to packaging in 95% N2 + 5% this quality loss. Cut surface discoloration or
O2 at 2 °C (36 °F) had no deleterious effect and enzymatic browning, caused by formation of
microbial counts were lowered throughout storage quinones in the presence of O2 and PPO, has been
(Ayhan et al. 1998). the subject of much research (Vámos-Vigyázó
1981, Sapers 1993). Since sulfite use as an anti-
Hydrogen peroxide (H2O2) is a strong oxidizing browning agent in the United States requires
agent and a powerful surface-contact sterilizer. It labeling for fresh produce, alternative GRAS and
has been shown experimentally to reduce microbe experimental compounds have been investigated.
populations on the surface of many produce items Nevertheless, enzymatic browning still represents
with minimal to no residue (Bolin and Huxsoll a major challenge with fresh-cut fruit (Weller et al.
1989, Sapers and Simmons 1998). However, the 1997, Sapers and Miller 1998).
612
Ascorbate, citrate, isoascorbate, and sodium isoascorbic acid, N-acetylcysteine, and calcium
erythorbate are some of the most commonly propionate) and held at 5 °C (41 °F) maintained
used agents to reduce or eliminate cut-surface visual quality for 5 weeks, yet microbial decay
discoloration. Ascorbate was more effective was evident after 4 weeks (Buta et al. 1999).
than erythorbate in preventing surface browning Analyses of organic acids and sugars revealed that
in ‘Winesap’ and ‘Red Delicious’ apple plugs slices treated with combinations of antibrowning
stored 24 h, and 1% citric acid enhanced their compounds retained higher levels of malate and
effectiveness (Sapers and Zoilkowski 1987). Cut had no decrease in sugar levels at 5 and 10 °C
surface discoloration was restricted in vacuum- (41 and 50 °F), indicating that higher quality was
packed carambola slices stored at 4 °C (39 °F) maintained during storage. Fresh-cut mangoes
that were treated with 1 or 2.5% citrate plus treated with 1 mM 4-hexylresorcinol, 50 mM
0.25% ascorbate (Weller et al. 1997). Ascorbate- potassium sorbate, and 500 mM ascorbic acid
2-phosphate and ascorbate-2-triphosphate also in MAP at 10 °C (50 °F) maintained color and
decreased cut surface discoloration in ‘Red sensory characteristics, with low microbial
Delicious’ apple plugs for 24 h (Sapers et growth, for 14 days (Gonzalez-Aguilar et al.
al. 1989). ‘Fuji’ apple slices treated with 2% 2000). Cut-surface discoloration was significantly
ascorbate had no browning or loss of visual reduced in fresh-cut banana slices treated with
quality for up to 15 days when stored at 10 °C 500 mM citrate and 50 mM N-acetylcysteine and
(50 °F) in 0.25% O2 (Gil et al. 1998). Calcium, stored at 5 °C (41 °F) or 15 °C (59 °F) for 7 days,
in combination with ascorbate, was effective and no microbial decay was observed (Moline
in preventing discoloration of fresh-cut apples et al. 1999). A combination of 0.5% carrageenan
(Ponting et al. 1972) and pears (Rosen and Kader and 0.5% citrate also inhibited browning in diced
1989, Gorny et al. 1998a). Browning was also ‘Granny Smith’ and ‘Red Delicious’ apples for 7
reduced in fresh-cut ‘Carnival’ peaches treated to 9 days at 3 °C (37 °F) (Tong and Hicks 1991).
with 1% calcium lactate plus 2% ascorbate (Gorny
et al. 1999). Cysteine inhibits PPO-mediated enzymatic
browning (Joslyn and Ponting 1951, Molnar-Perl
Browning was retarded in slightly under-ripe and Friedman 1990, Gunes and Lee 1997). Three
‘Bartlett’ and ‘d’Anjou’ pears treated with a mechanisms have been proposed to explain how
combination of sodium erythorbate, CaCl2, and thiol compounds inhibit enzymatic browning:
4-hexylresorcinol after 14 days storage at 4 °C reduction of o-quinone back to o-dihydroxyphenol
(39 °F). However, fresh-cut ‘Bosc’ pears browned (Kahn 1985); direct inhibition of PPO (Dudley
severely irrespective of inhibitor treatment and Hotchkiss 1989, Robert et al. 1996); and
(Sapers and Miller 1998). On the other hand, a formation of a colorless cys-quinone adduct
combination dip with 0.01% 4-hexylresorcinol, (Richard et al. 1991).
0.5% ascorbate, and 1% calcium lactate extended
shelf-life of ‘Anjou,’ ‘Bartlett,’ and ‘Bosc’ pear When cysteine is used as an inhibitor of
slices for 15 to 30 days (Dong et al. 2000). enzymatic browning on sliced apples (Walker and
Furthermore, combined treatments with 1 mM Reddish 1964) or pears (Sapers and Miller 1998),
4-hexylresorcinol, 0.5 M isoascorbate, 50 mM pinkish-red off-colored compounds are formed
potassium sorbate, and 25 mM N-acetylcysteine due to phenol regeneration with deep
decreased browning in fresh-cut ‘Anjou,’ color formation (Richard-Forget et al. 1992). If
‘Bartlett,’ and ‘Bosc’ pears for 14 days. The off-color formation can be prevented, cysteine
preservative effect was unaffected by initial may prove to be an effective replacement to
firmness (4.7 to 11.7 lb, 21 to 52 N) for ‘Anjou’ bisulfites. Cysteine is a naturally occurring
slices (Buta and Abbott 2000). amino acid that has GRAS status for use as a
dough conditioner (Code of Federal Regulations
‘Red Delicious’ apple slices treated with a 21:184.1271 and 21:184.1272). Development of
combined antibrowning dip (4-hexylresorcinol, cut surface discoloration was reduced for only
613
1 day at 0 °C (32 °F) in ‘Golden Delicious’ fresh- and increased color stability for up to 16 days at
cut apples treated with 0.1% cysteine (Nicoli et al. abusive storage temperatures (Corbo et al. 2000).
1994). Ineffectiveness of the cysteine treatment Research is currently in the initial stages for the
was attributed to oxidation in the package and use of volatile compounds on fresh-cut products;
likely was due to the low concentration applied. hexanal is not currently approved for use.
Gorny et al. (2002) reported that a post-cutting
dip (pH 7.0) of 2% ascorbate, 1% calcium Methyl jasmonate is a naturally occurring
lactate, plus 0.5% (weight by volume) cysteine volatile compound, found in many plants, that
significantly extended shelf-life of ‘Bartlett’ has hormonelike activity at low concentrations.
pear slices by inhibiting loss of firmness and Exogenously applied methyl jasmonate is effective
preventing browning. Consumer panelists could in reducing mold growth on fresh-cut celery and
not distinguish between pear slices treated with peppers and may have applications as a naturally
the preservative and controls. After 10 days in air derived fungicide (Buta and Moline 1998).
at 0 °C (32 °F), 82 and 70% of consumers judged
treated pear slices to be acceptable in appearance Edible coatings have been used in an attempt to
and flavor, respectively. preserve fresh-cut products because the coatings
act as barriers to water loss and gas exchange,
When used in combination with ascorbic acid, creating a micromodified atmosphere around
4-hexylresorcinol is an effective inhibitor of cut products, and can serve as carriers for other GRAS
discoloration on many fresh-cut fruit, including compounds (Baldwin et al. 1995a). Ethylene
apples and pears especially ( Monslave-Gonzalez production and CO2 evolution were reduced in
et al. 1993, Luo and Barbosa-Cánovas 1996, 1997, apple slices coated with double layers of buffered
Sapers and Miller 1998, Moline et al. 1999, Buta polysaccharide/lipid, stored at 23 °C (73 °F)
and Abbott 2000, Dong et al. 2000). Between 1 (Wong et al. 1994). Use of a cellulose-based
and 7 µL L-1 of residual 4-hexylresorcinol was edible coating on fresh-cut apple cylinders stored
necessary to prevent browning on fresh-cut pear in overwrapped trays at 4 °C (39 °F) increased
slices stored up to 14 days at 2 to 5 °C (36 to 41 shelf-life by about 1 week (Baldwin et al. 1996).
°F) (Dong et al. 2000). Although it is effective in The effectiveness of ascorbate to reduce browning
preventing cut surface browning, it is not currently and potassium sorbate to decrease microbial
considered GRAS by the FDA and may not be growth was superior when incorporated into this
used on fresh-cut fruit. It may also impart an edible coating. A commercially available sucrose
unacceptable off flavor on fruit products. ester edible coating also inhibits browning of
fresh-cut fruit by acting as an O2 barrier.
Antimicrobials, Edible Coatings, and Other Peeled, packaged citrus products have a shelf-life
Treatment Compounds of approximately 17 to 21 days, but fluid leakage
can be problematic. Edible wax microemulsion
Hexanal is a natural aroma precursor in apples that coatings (up to 12% SSC) reduced leakage of dry-
is readily converted to aroma volatiles in vivo by packed grapefruit segments by 80% after 2 weeks
fresh-cut apple slices (Song et al. 1996). Hexanal and by 64% after 4 weeks (Baker and Hagenmaier
can not only enhance aroma, but it also reduced 1997). Coatings could be made with polyethylene,
enzymatic browning at cut surfaces, as well as candelilla, or carnauba wax with lauric, stearic,
inhibiting molds, yeasts, and mesophilic and palmitic, oleic, or myristic acids. Carnauba wax
psychrotrophic bacteria in ‘Granny Smith’ slices was most effective, and coatings were not detected
stored at 15 °C (59 °F) (Lanciotti et al. 1999). The by informal taste panels (Baker and Hagenmaier
inclusion of hexanal and (E)-hexenal in the MAP 1997).
(70% N2 + 30% CO2) of sliced ‘Granny Smith’
apples reduced spoilage microbe populations
614
Compendium of Recommendations and
Data Related to Fresh-Cut Fruit Products
Table 1. Respiration rates for fresh-cut fruits in air at various storage temperatures
________________________________________________________________________________
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production, multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
615
Table 2. Storage atmosphere recommendations for selected fresh-cut fruits
________________________________________________________________________
Atmosphere
Fresh-cut product Temperature O2 CO2 Efficacy
________________________________________________________________________
ºC %
616
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623
Fresh-Cut Vegetables contribute to tissue browning. These changes are
managed by the use of low temperatures, creation
M. Margaret Barth, Hong Zhuang, and Mikal E. of reduced O2 or elevated CO2 atmospheres, and
Saltveit application of inhibitors of specific chemical
reactions or metabolic pathways.
Barth and Zhuang are with Redi-Cut Foods, Inc.,
Franklin Park, IL; Saltveit is with the Department Wound-enhanced respiration and ethylene
of Plant Sciences, University of California, Davis, production can deplete carbohydrate storage
CA. reserves and stimulate tissue softening associated
with fruit ripening (melons, squash, tomatoes)
or chlorophyll loss associated with leaf or
Introduction tissue senescence (spinach, broccoli). In many
tissues, wounding produces a signal that induces
Wounds inflicted during the preparation of the increased synthesis and accumulation of
fresh-cut vegetables promote many physical and phenolic compounds. Tissue browning can
physiological changes that hasten loss of product result from the oxidation or polymerization of
quality (Brecht 1995, Saltveit 1997). Foremost accumulated phenolic compounds. Lignification
among these are the removal of the protective and toughening of tissue are others way in which
epidermal layer and exposure of internal cells. the metabolism of phenolic compounds can reduce
These changes not only facilitate water loss but quality.
also provide easy entry for microbial pathogens
and chemical contaminants. Packaging or Sanitation, implementation of Good Agricultural
application of edible films can lessen water loss Programs (GAPs) in the field, use of Good
by maintaining a high RH near the cut surface Manufacturing Programs (GMPs) and Hazard
and providing a physical barrier that protects the Analysis and Critical Control Point (HACCP)
product from contamination. monitoring plans during all steps of processing,
and proper temperature control, are all needed to
Water loss and collapse of injured cells at the ensure that an initial low microbial load on fresh-
cut surface can alter the appearance of the fresh- cut vegetables is maintained during marketing.
cut product. As the cut surface loses water, The current industry standard washing and
adhering cellular debris may impart a white disinfection procedures used on whole produce
blush to the surface that masks varietal color; for are rather ineffective with fresh-cut vegetables
example, white blush on “baby” carrots decreases once they have been contaminated with microbial
the intensity of the underlying orange color. pathogens. Overall microbial load relative to
Differential dehydration of exposed cortex and spoilage organisms usually remain below levels
vascular tissue may produce an uneven surface, of concern as long as visual quality remains
as with formation of vascular strands projecting acceptable. However, postharvest treatments
a few millimeters from the cut end of celery that maintain visual quality under abusive
petioles. Consumers associate both of these temperatures may allow microbial loads to reach
surface changes with the loss of freshness. dangerous levels before quality is reduced below
acceptable levels.
Physiological processes devoted to wound repair
can be beneficial (such as curing potatoes) or Many vegetables are sensitive to nonfreezing
detrimental to quality retention. Physiological temperatures below about 10 °C (50 °F) and
changes following wounding include increased suffer physiological damage if held at these
respiration and ethylene production, promotion of chilling temperatures beyond a specific period.
the ripening of climacteric fruit vegetables (such These vegetables include jicama, pepper, sweet
as melons and tomatoes), and enhanced synthesis potato, tomato, and zucchini. In contrast to the
and accumulation of phenolic compounds that whole commodity, which should not be chilled,
624
the increased susceptibility of fresh-cut products slightly reduced during storage in 5% O2 and 5%
to spoilage often means that the product has a CO2 at 5 °C (41 °F).
longer market life at 0 °C (32 °F) than at higher,
nonchilling temperatures. Development of chilling
injury symptoms is less pronounced in riper Respiration Rates
tissue and takes some time to develop. Since
most fresh-cut vegetables are consumed soon Temperature Whole Cubed Grated
after purchase and fruit vegetables are fully ripe peeled 1 cm cubes 2 mm
when processed, chilling injury symptoms usually mg CO2 kg-1 h-1
do not develop to a significant extent because 2 °C 4 10 12
the rate of deterioration due to chilling is slower 5 °C 6 12 16
than the rate of spoilage that is inhibited by low 10 °C 19 27 38
temperatures. The two most important fresh-cut 23 °C 54 117 162-207
commodities, lettuce and carrots, are not chilling Data from Gorny (1997).
sensitive and should be stored as close to 0 °C (32
°F) as possible without freezing: 1 to 3 °C (34 to To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
38 °F) in a commercial setting. by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production,
The imposed physical damage done to the multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
product during preparation and its increased or by 61 to get kcal tonne-1 day-1.
vulnerability to deleterious internal and external
changes requires that fresh-cut vegetables be
handled with a greater degree of care than the
Broccoli (Florets)
whole product. Storage requirements for intact
produce may be inadequate to handle increased
Fresh-cut broccoli florets should be tight, firm,
physiological activity and susceptibility to water
turgid, and dark green without blooming buds.
loss encountered with fresh-cut vegetables.
There should be no sulfur odor or discoloration
along the stems and cut ends. The core
The highest quality that is available (usually
temperature of raw material should be <1.5 °C
USDA No. 1) and that is compatible with an
(35 °F). Raw and processed material should be
economic return should be used for processing.
stored at 1 to 3 °C (34 to 38 °F) to ensure quality
Although poor quality pieces of fresh-cut product
and reduce potential for freezing of product during
can be discarded to upgrade quality (an example
handling, distribution, and storage. Yellowing is
is culling discolored pieces of cut lettuce leaves
a common problem caused by either chlorophyll
or broccoli florets), the associated cost usually
loss or blooming of the buds. The cut surface
adversely affects quality and yields and greatly
and damaged floret stems can turn black during
outweighs the differential cost of using higher
storage. Development of off odors can be a major
quality raw material.
concern when MAP is used. Temperature abuse
promotes soft rot and mold growth.
The remainder of this chapter provides
information on the commercial packaging and
Whole broccoli heads are hand-cut into florets that
storage of a number of fresh-cut vegetables.
are between 2.5 cm (1 in) and 5 cm (2 in) long.
They are washed in water containing up to 200 µL
L-1 total chlorine to wash residual material from
Beets, Red (Grated, Cubed, Whole Peeled)
the florets as well as reduce aerobic plate counts.
Repeated daily washing did not maintain lower
Fresh-cut beets should be stored at 1 to 3 °C (34 to
microbial counts for >2 days.
38 °F) before and after processing. Respiration is
625
The benefit of CA (5% O2 and 4% CO2) may be should be stored at 1 to 3 °C (34 to 38 °F) to
marginal for storage up to 14 days at 0 to 5 °C ensure quality and reduce potential for freezing of
(32 to 41 °F) compared to air. Lowering O2 to product during handling, distribution, and storage.
0.25% or increasing CO2 to 10% at 0 to 5 °C (32 Fresh-cut cabbage includes diced and shredded
to 41 °F) reduces respiration by about 50%. Use product with cut size varying from 0.63 cm (¼ in)
of appropriate polymeric film in MAP maintained to 0.95 cm (3/8 in). Heads are trimmed to remove
green color at 0 to 5 °C (32 to 41 °F) for more wrapper leaves and cored, cut, and washed using
than 21 days (Cabezas and Richardson 1997). chlorinated water (100 µL L-1 of total chlorine)
Severe off odors and discoloration at cut ends for about 1 min before being spun-dried and
can develop during MAP storage at 10% CO2 and packaged. For coleslaw, cut cabbage (shredded
2.5% or less O2 (Makhlouf et al. 1989). Perforated or diced) can be preblended with carrots or plain-
and microperforated polymeric packages reduce packaged.
off odor (Izumi et al. 1996a). Reducing ethylene
below 1 to 10 µL L-1 did not significantly reduce A CA of 5 to 7.5% O2 and 15% CO2 is
color loss at 1 °C (34 °F), but did have an effect at recommended (Hiroaki et al. 1993). Lowering
higher temperatures. O2 below 5% caused rapid proliferation of
fermentative bacteria and off odors within 6 days
No foodborne disease outbreak has been reported at 5°C (41 °F). The fermentation induction point
associated with fresh-cut broccoli, although the of coleslaw mixes in low density polyethylene
aerobe population is usually high (>100,000 cfu bags varied with the cabbage to carrot ratio and
g-1 fresh weight). Florets stored in 5% O2 and with temperature. At 5 °C (41 °F), a 70:30 mix
8% CO2 at 8 °C (46 °F) have lower aerobic plate went anaerobic at 1.8% O2, while 3% O2 was the
counts, total coliform plate counts, and yeast/mold limit for aerobic respiration at 10°C (50 °F).
plate counts compared to storage in air.
Off odors forming in cut cabbage bags with
low OTR film (<3,000 mL/m2 atm-1 d-1) and
Respiration Rates discoloration of cabbage leaf packed in high OTR
film (>12,000 mL/m2 atm-1 d-1) bags are the major
Temperature mg CO2 kg-1 h-1 quality deterioration during storage (Pirovani et
0 °C 26 al. 1997). At 11 °C (52 °F), Listeria spp. grew
5 °C 44 faster in fresh-cut cabbage packages that had an
10 °C 78 atmosphere of <1.8% O2 and >20% CO2 than in
Data from Watada et al. (1996) and Izumi et al. air (Omary et al. 1993).
(1996a).
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate Respiration Rates
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
1.8 at 20 °C (68 °F). To calculate heat production, Temperature 3/8 in (9.6 mm) 1/4 in (6.4 mm)
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 mg CO2 kg-1 h-1
or by 61 to get kcal tonne-1 day-1. 2 °C 16-18 18-24
5 °C 22-33 25-39
10 °C 42-48 51-57
Cabbage, Green and Red (Shredded, 23 °C 117-153 153-171
Diced) Data from Watada et al. (1996).
Fresh-cut green cabbage should be light green To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
with a moderately pungent flavor and no sulfur by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
aroma. Raw and processed material should have 1.8 at 20 °C (68 °F). To calculate heat production,
typical cabbage flavor with no off notes and
626
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 surface also prevent white blush development
or by 61 to get kcal tonne-1 day-1. (Cisneros-Zevallos et al. 1997).
629
Butterhead Lettuce Greenleaf Lettuce
Browning of the cut edges of butterhead lettuce is A 0.5 to 3% O2 and 5 to 10% CO2 CA reduced cut-
reduced by modified atmospheres rapidly created edge browning of greenleaf lettuce (Lopez-Galvez
by flushing with N2 to get 1 to 3% residual O2 with et al. 1996).
CO2 levels of 5 to 10%. Tissue in atmospheres
of <1% O2 and >10% CO2 develop CO2 injury
or brown stain (Stewart and Ceponis 1968). The Iceberg Lettuce
incidence of brown stain increased from 16 to 38
to 81% as CO2 increased from 2.5 to 5 to 10%, The raw product should have <6% pink rib
respectively, and was enhanced when combined discoloration, while russet spotting, hooked core,
with low O2 atmospheres (Steward and Uota and tipburn should be <1%. Moisture content of
1971). lettuce is extremely important for maintaining
texture and reducing microbial growth. Excess
The physiological behavior of butterhead lettuce moisture can be removed in a forced-air tunnel or
is modified by cultural practices such as irrigation, by spinning. Lettuce that was air dried for 10 to
climate, and fertilization (Poulsen et al. 1994, 15 min or spin dried for 30 min had less browning
1995, Sorensen et al. 1994). Susceptibility to during storage than control samples. Consumer
brown stain varies among cultivars and position of sensory panelists indicated that after 4 days of
leaves within the head (Krahn 1977). Shelf-life of storage, only 15% would purchase control lettuce
butterhead lettuce was negatively correlated with while 40% would purchase lettuce spin- or air-
respiration rate and susceptibility to CO2. High dried for 15 min. Slicing methods can influence
O2 and low CO2 enhanced enzymatic browning. ascorbic acid retention in with the order: manual
Off odors and flavors developed (ethanol, tearing > manual slicing > machine slicing.
acetaldehyde, and ethyl acetate aroma), crispness
was lost, and the tissue became translucent when In clamshell trays, market-life of fresh-cut lettuce
fresh-cut lettuce was stored in <0.5% O2 or >15% was <24 h at 23 °C (73 °F), >24 h at 10 °C (50
CO2. °F), and >48 h at 2 °C (36 °F) (Zhuang and Barth
1999). Fresh-cut lettuce packages commonly have
Necrosis of butterhead lettuce may be associated <1% O2 to effectively slow browning and >10%
with microbial growth, such as pectolytic CO2 to inhibit microbial growth (Gorny 1997). An
Pseudomonas (Nguyen-The and Prunier 1989) and atmosphere of 0.5 to 3% O2 and 10 to 15% CO2
other epiphytic bacteria. reduces cut edge browning, retains visual quality,
and reduces psychrophilic bacterial counts.
Flushing with 100% N2 increases retention over
Respiration Rates (Chopped) MAP. On the basis of ethanol appearance in the
headspace, the fermentation induction point was
Temperature mg CO2 kg-1 h-1 0.3 to 0.4% O2 at 5 °C (41 °F) and 0.6 to 0.8%
2 °C 12-14 at 10 °C (50 °F). Acetaldehyde and ethyl acetate
4.5 °C 20-25 were detected when O2 was <0.4% at 5 to 20 °C
10 °C 38-48 (41 to 68 °F). The extent of browning increased
Data from Gorny (1997). with O2 level but not with CO2 level (Smyth-Anne
et al. 1998). Fresh-cut iceberg should be stored at
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate 1 to 3 °C (34 to 38 °F) before and after processing
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and to ensure quality and reduce potential for freezing
1.8 at 20 °C (68 °F). To calculate heat production, during handling, distribution, and storage.
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1. Shelf-life of fresh-cut lettuce at 5 °C (41 °F) was 6
days in air, 8 days in 3% O2, and 12 days in 0.2%
630
O2 (Peiser et al. 1997). Increasing CO2 enhances Lollo Rosso (Red) Lettuce
the beneficial effect of low O2. A combination
of 0.2% O2 with 7 to 15% CO2 gave a 16-day The beneficial storage atmosphere is 0.5 to 3%
shelf-life. A 14-day shelf-life was obtained when O2 and 5 to 10% CO2. MAP storage (2 to 3% O2
shredded iceberg lettuce was stored in 1 to 3% O2 and 12 to 14% CO2) was useful in preventing
and 5 to 6% CO2 at 5 °C (41 °F). However, CO2 brown discoloration. However, MAP storage was
>15% causes brown stain. Reduced O2 or elevated not beneficial for preserving quality of red tissues
CO2 may induce formation of fermentation and and, in fact, their overall visual quality, texture,
off odor (fermentative volatiles, ethanol, and aroma, and macroscopic breakdown under MAP
acetaldehyde) in the packages (McDonald et al. conditions were worse than those of air-stored
1990, Mateos et al. 1993, Peiser et al. 1997). tissues (Gil-Maria et al. 1998). Castaner et al.
(1997) found that after 14 days of storage at 5
Although gram-negative bacteria are numerically °C (41 °F), the overall visual quality of fresh-
dominant, a large yeast population may also cut white and green tissue of Lollo Rosso (red)
be found. Species of Pseudomonas, Erwinia, lettuce is better in MAP than in air. Green tissue
and Serratia were the most frequently isolated had the lowest overall quality after air storage.
bacteria. Cryptococcus, Pichia, Torulaspora, and In addition, there were no significant overall
Trichosporon spp. were the most common yeasts. quality differences for red tissue stored under
Beuchat and Brackett (1990) studied the effects either condition. Texture and aroma were excellent
of shredding, chlorine treatment, and MAP on for green tissue held in MAP, while it was
survival and growth of Listeria monocytogenes, unacceptable in air. Both atmospheres maintained
mesophilic aerobes, psychrotrophs, yeasts, and texture of red and white tissues. Aroma was
molds. They found that no significant changes in excellent for white tissue in both air and MAP. In
populations of L. monocytogenes were detected contrast, aroma was only acceptable for red tissue
during the first 8 days of incubation at 41 °F (5 stored in air.
°C); there was a significant increase between 8
and 15 days. Significant increases occurred within
3 days when lettuce was stored at 50 °F (10 °C). Romaine Lettuce
Chlorine treatment, MAP of 3% O2, and shredding
did not influence growth of L. monocytogenes. Fresh-cut romaine should be stored at 1 to 3
°C (34 to 38 °F) before and after processing to
ensure quality and reduce potential for freezing of
Respiration Rates (Shredded) product during handling, distribution, and storage.
MAP exerted a beneficial effect on sensory quality
Temperature mg CO2 kg-1 h-1 by preventing pink discoloration. Compared to
2.5 °C 12.0-15.0 air, 3% O2 and 6, 10, or 14% CO2 MAP delayed
5 °C 15.6-27.3 development of tissue discoloration and increased
7.5 °C 23.1-32.7 shelf-life by about 50%. Of the three CO2 levels,
10 °C 30.4-39.9 10% was slightly more effective than 6 and 14%
Data from Gorny (1997). (Hamza et al. 1996, Lopez-Galvez et al. 1996,
Segall and Scanlon 1996).
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and The aerobic plate counts of fresh-cut Romaine
1.8 at 20 °C (68 °F). To calculate heat production, lettuce in MAP were decreased by 0.15 and 0.35
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 kGy gamma irradiation (Prakash et al. 2000). The
or by 61 to get kcal tonne-1 day-1. 0.35 kGy treatment decreased aerobic plate counts
by 1.5 logs and yeast and mold counts by 1 log.
A combination of MAP and 0.35 kGy resulted
in a 2-log reduction in microbial counts. These
631
differences were maintained through 22 days of multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
storage. Irradiation at 0.15 kGy caused smaller or by 61 to get kcal tonne-1 day-1.
reductions in microbial counts. A 10% loss in
firmness was observed at 0.35 kGy, while other
sensory attributes such as color, generation of off Onions (Diced, Slivered, Rings, Chunks)
flavor, and appearance of visual defects were not
affected. The control sample reached microbial Fresh-cut onions should have no discoloration,
counts of 107 in 14 days compared to the MAP skin and core. Bulbs should be dry, free of
samples, which reached the same level in 17 decay, firm, and 7.5 to 10 cm (3 to 4 in) in
days, and the combination samples (0.35 kGy and diameter. The core of whole onions should be
MAP), which took 28 days. Color, flavor, and odor 1.5 °C (35 °F) at receiving and 1 to 3 °C (34 to
were unaffected by irradiation, although an off 38 °F) before and after processing to ensure
odor was obvious in MAP samples after 21 days quality and reduce potential for freezing of
(17% CO2 and <1% O2). Low-dose irradiation product during handling, distribution, and storage.
increased shelf-life of cut romaine lettuce by 2 to The seed stems and new internal growth should
5 days (Prakash et al. 2000) be less than 10%; double centers and translucent
scale should be less than 5%.
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate CA of 2 to 5% O2 and 10 to 15% CO2 decreases
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and respiration and microbial proliferation and retains
1.8 at 20 °C (68 °F). To calculate heat production, sucrose and pungency of cut onions. Lowering
632
the O2 level can be beneficial for visual quality To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
but has no effect on aroma. Enrichment in CO2 by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
improves both qualities. Rapid reduction of O2 in 1.8 at 20 °C (68 °F). To calculate heat production,
the package reduces discoloration. Browning can multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
be slowed slightly by lowering O2, and more so or by 61 to get kcal tonne-1 day-1.
by CO2 enrichment. Langerak (1975) reported that
freshly cut onion became brown and unacceptable
after cooking when wrapped in perforated film, Pepper, Bell (Diced, Sliced)
in comparison with onion wrapped in film
that achieved MAP conditions more rapidly. Fresh-cut bell pepper should have no discoloration
Atmospheres containing 1 to 2% O2 prevent the (darkening) or water soaking. Raw material should
loss of pyruvic acid, an indicator of onion flavor be received at <7 °C (45 °F) and stored at 7 to
intensity, and reduce the rate of respiration of the 10 °C (45 to 50 °F), but stored at 1 and 4 °C (34
fresh-cut tissue (Mencarelli et al. 1990). to 40 °F) after processing to ensure quality and
reduce potential for freezing of product during
The inclusion of potassium permanganate (as an handling, distribution, and storage. Defects that
ethylene scrubber) and sulfur volatiles in packages reduce overall visual quality include darkening of
of diced onions improves sensory quality. A the green or red pulp, brown discoloration of the
storage life of 10 days is possible for fresh-cut cut surfaces, and decay. Whole peppers should
onions packaged in polymeric bags without a be spray-washed before processing and washed
gas flush and stored at 2 °C (36 °F). Addition of after being cored and cut to reduce microbial
absorbents, potassium permanganate and clay, in population. Alternatively, cut pepper can be
packages increased shelf-life up to 18 days at 1 °C washed using a combination of open-flume and
(34 °F) (Howard et al. 1994, Toivonen 1997). close-flume systems.
Yeasts and molds were virtually absent on diced Although intact peppers are chilling sensitive,
onion. When present, their count did not increase it is necessary to store fresh-cut red and green
beyond 100 cfu g-1 (Garg et al. 1990, Blanchard pepper at 0 to 5 °C (32 to 41 °F) to maintain visual
et al. 1996). Psychrotrophic and mesophilic flora quality (El-Bassuoni and Cantwell 1994). Visual
increased throughout storage under all treatments. quality was maintained and few compositional
Their growth in diced onion was slowed under CA changes occurred during 15 days of storage at 1
as compared to air (Blanchard et al. 1996). °C (34 °F) (Abe et al. 1991, Zhou et al. 1992).
Full ripe or red peppers are less chilling sensitive
Respiration rates vary significantly depending on than mature-green fruits of the same cultivar.
how long whole onions were in cold storage prior
to processing. CA (5% O2 and 5% CO2) at 5 °C The recommended CA is 3% O2 and 5 to 10%
(41 °F) reduces respiration rate by 25%. CO2. Levels of CO2 >10% can result in tissue
darkening and softening. Shelf-life and sensitivity
to CO2 injury vary significantly among cultivars.
Respiration Rates CA using air with 10% CO2 maintains quality
at both 5 and 10 °C (41 and 50 °F). Aroma and
Temperature Sliced Diced texture decrease after 6 days in 10% CO2 but were
2 mm thick rings 1 cm cubes maintained in air at 0°C. Storage in high CO2 at
mg CO2 kg-1 h-1 5 °C (41 °F) and air at 0 °C (32 °F) retard decay
2 °C 14.0 12.0 development. Atmospheres with 5% CO2 help
5 °C 23.4 15.6 maintain quality but are not as effective as 10%
10 °C 38.0 22.8 CO2. Fresh-cut pepper pieces that were ripening
23 °C 126-131 90-99
Data from Gorny (1997).
633
or were already fully red ripe had better shelf- in the cooked product that result in surface
life than pieces from mature-green fruit (Lopez- firming (case hardening) and partial separation
Galvez et al. 1997). They also found that visual of the case-hardened superficial layer from
quality was best maintained in air or CA at 0 °C underlying tissue during slicing. Furthermore,
(32 °F), with important quality loss observed by case-hardened potatoes form large lumps during
day 9 at 5 °C (41 °F) and by day 3 at 10 °C (50 mashing (Martin et al. 1999). The recommended
°F). Decay is caused by both fungi (Alternaria and storage temperature is 0 °C (32 °F). Shelf-life of
Botrytis spp.) and soft-rot-causing bacteria. Diced minimally processed products can be extended to
tissue was more susceptible to decay than slices 3 weeks under CA and refrigeration.
(Lopez-Galvez et al. 1997).
Dipping potato strips in chlorine solutions
(100 and 300 µL L-1) result in higher microbial
Respiration Rates populations during storage, while potatoes
treated with antibrowning solutions combined
Temperature Sliced Diced with MAP showed only a slight increase.
5 cm 1 cm cubes MAP had no significant effect on microbial
mg CO2 kg-1 h-1 populations compared to nonpackaged samples.
0 °C 2-6 2-9 The predominant organism was Pseudomonas
5 °C 4-7 6-12 fluorescens (Gunes et al. 1997).
10 °C 6-13 11-18
Data from Gorny (1997). The recommended CA is 1 to 3% O2 and 6 to 9%
CO2. Reducing O2 level to 10% or 3%, with 10%
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate CO2, at 2 °C (36 °F) reduces respiration of potato
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and sticks by 50 to 75%. However, 5% O2 and 5%
1.8 at 20 °C (68 °F). To calculate heat production, CO2 at 5°C (41 °F) increases respiration of sticks
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 during storage.
or by 61 to get kcal tonne-1 day-1.
Active modification of package atmospheres
is necessary to prevent browning and achieve
Potato (Sticks, Diced, Sliced, Peeled) extended shelf-life. MAP alone will not prevent
browning (Gunes and Lee 1997). Vacuum
Fresh-cut potatoes should be firm and without packaging of fresh-cut potatoes may create
brown discoloration. Enzymatic browning is a anaerobic conditions that allow growth of
major problem in the discoloration of peeled or Clostridium botulinum.
fresh-cut potatoes. Hand-peeling and lye-peeling
result in good quality, while abrasion-peeling is
undesirable for fresh potatoes. Anti-browning
dips such as 0.5% L-cysteine and 2% citrate, in
combination with MAP, are needed to prevent
browning of whole peeled potatoes. Pretreatment
with heated ascorbate and citrate solutions,
prior to a browning inhibitor dip containing
4% ascorbate, 1% citrate, and 1% sodium
pyrophosphate, has greatly extended storage life
over that after the dip alone (Sapers and Miller
1995, Sapers et al. 1997). However, under some
conditions, such pretreatment-induced changes
634
Respiration Rates
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate by 2.0 at 0 °C
(32 °F), 1.9 at 10 °C (50 °F), and 1.8 at 20 °C (68 °F). To calculate
heat production, multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1
or by 61 to get kcal tonne-1 day-1.
Fresh-cut rutabaga should have uniform yellow Fresh-cut spinach should be green without any
flesh without decay, bruises, or discoloration. Raw decay or bruise. Raw material should arrive at
material should arrive at <7 °C (45 °F), while raw 1.5 °C (35 °F). Fresh and processed products
and fresh-cut product should be stored at 1 to 3 °C should be stored at 1 to 3 °C (34 to 38 °F) before
(34 to 38 °F) before and after processing to ensure and after processing to ensure quality and reduce
quality and reduce potential for freezing during potential for freezing of product during handling,
handling, distribution, and storage. A CA of 5% distribution, and storage.
CO2 and 5% O2 is slightly beneficial and reduces
respiration at 5 °C (41 °F). Storage in 0.8 to 3% O2 and 8 to 10% CO2 is
beneficial. Spinach stored in 0.8% O2 at 20°C
(68 °F) had a lower respiration rate, superior
Respiration Rates appearance, and better taste than spinach leaves
stored in air (Izumi et al. 1997). Atmospheres of
Temperature Quarters Shredded Diced 0.8% O2 or 0.8% O2 and 10% CO2 reduced the
peeled 2 mm 1 cm cubes number of aerobic mesophilic and psychrotrophic
mg CO2 kg-1 h-1 microorganisms on fresh-cut spinach leaves by
2 °C 8 16 18 10- to 100-fold, compared to air-stored leaves at
5 °C 12 20 25 5 °C (41 °F); however, there was no effect at 10
10 °C 15 42 42-48 °C (50 °F). These atmospheres had no effect on
23 °C 45 72-81 153-162 texture changes during storage at 5 °C (41 °F) for
Data from Gorny (1997). 9 days or at 10 °C (50 °F) for 7 days (Babic and
Watada 1996).
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and An atmosphere of 4% O2 and 9% CO2 reduced
1.8 at 20 °C (68 °F). To calculate heat production, the loss of ascorbic acid by 50% compared to
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 air (Burgheimer et al. 1967). Increasing CO2
concentrations up to 13% caused a rapid decrease
or by 61 to get kcal tonne-1 day-1.
in ascorbic acid and development of off odors that
635
were not acceptable after 1 week storage at 7 °C A 6.5% CO2 and 12% O2 atmosphere is slightly
(45 °F) (McGill et al. 1966). Chlorophyll loss can beneficial.
be reduced by 50% with ethylene scrubbing. Low
O2 (0.8%) results in a slightly reduced respiration
rate of cleaned spinach leaves. Tomato (Sliced, Diced)
Microbiological and sensory changes occur Fresh-cut tomatoes should have consistent red
in all packaged spinach samples, being color and firm texture. Sliced tomatoes have no
more pronounced at 10 °C (50 °F) than at gel loss from the seed cavities. Diced tomatoes
4 °C (40 °F). Spinach leaves harbor high have no seeds or stem pieces. ‘Roma’ tomatoes
numbers of mesophilic, psychrotrophic, and are often used for diced tomatoes; the ripening
Pseudomonadaceae bacteria (Babic and Watada stage should be 5 or higher (USDA standard).
1996). The pectinolytic species Pseudomonas The ripening stage of round tomatoes for slicing
fluorescens is probably the major spoilage agent of should be 5.5 or higher. Fruit should be firm
fresh-cut spinach. Microbial populations on fresh- and have small seed cavities. For diced tomato
cut spinach leaves increase during storage in air products, ‘Roma’ tomatoes should be spray-
and CA at 5 and 10 °C (41 and 50 °F). Populations washed with chlorinated water before cutting.
of Enterobacteriaceae and Micrococcaceae are not After cutting, an open fluming system with 0.25%
greatly affected by storage atmosphere. CaCl2 and 100 µL L-1 chlorine is used to wash and
remove seeds. For sliced tomato products, whole
tomatoes are dipped in 200 µL L-1 chlorinated
Respiration Rates (Whole Leaves) water for more than 1 min before slicing. Various
automated slicing machines have been developed
Temperature mg CO2 kg-1 h-1 for tomatoes. Sliced tomatoes can be packed in
0 °C 6.0-14.0 stacking, shingling, or soldier style.
5 °C 11.7-23.4
Data from Gorny (1997). Although tomatoes are chilling sensitive, they can
be stored at 0 to 5 °C (32 to 41 °F) for a few days
To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate before processing to retard softening. A CA of
by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and 3% O2 and 3% CO2 will delay ripening as well as
1.8 at 20 °C (68 °F). To calculate heat production, SSC and TA losses (Mencarelli and Saltveit 1988).
multiply mg kg-1 h-1 by 220 to get BTU ton-1 day-1 Water soaking that produces translucent tissue,
or by 61 to get kcal tonne-1 day-1. textural changes, and softening reduces the quality
of diced tomatoes during storage. In addition to
these deleterious changes, quality of tomato slices
Sweet Potato (Shredded, Sliced) can be reduced by seed germination and loss of
locular gel. The high acidity of tomato products
Fresh-cut sweet potatoes should be firm and suppresses microbial growth, but growth of yeasts
without discoloration. Whole sweet potatoes are and molds can reduce quality in storage.
washed with water before peeling and shredding.
Cut sweet potatoes are then washed with 100 µL
L-1 sodium hypochlorite, rinsed, and centrifuged Respiration Rates
to remove excess water. Product quality remains
acceptable during 2 weeks storage at 0 to 4 °C (32 Temperature mg CO2 kg-1 h-1
to 40 °F) in polymeric bags. Color of shredded 20 °C 57.6-93.6
sweet potato darkens and firmness declines during
storage. β-Carotene remains stable at 7.8 mg per To get mL CO2 kg-1 h-1, divide the mg kg-1 h-1 rate
100 g at 4 °C (40 °F). Vitamin C remains stable by 2.0 at 0 °C (32 °F), 1.9 at 10 °C (50 °F), and
at 7 mg per 100 g for 1 week and then decreases. 1.8 at 20 °C (68 °F). To calculate heat production,
636
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641
Bedding Plants and Seedlings final destination (Lange et al. 1991b, Heins et al.
1994b, Kaczperski et al. 1996).
Hyeon-Hye Kim, Kevin Kern, and William H.
Growers who only have one or two coolers may
Carlson
need to store plugs of several species at one time.
Researchers in Michigan (Heins and Lange 1992d,
Kim, Kern, and Carlson are with the Department
Heins et al. 1992) showed how to identify a
of Horticulture, Michigan State University, East
compromise temperature—one that is collectively
Lansing, MI.
acceptable, although maybe not individually
optimal. If only short-term storage is necessary,
a temperature warmer than the optimum may be
Introduction
more economical (Heins et al. 1994b). Also, the
environmental conditions before and after storage
Storage of vegetable and flower bedding plants
can influence the growth of the plugs following
may be necessary if adverse weather conditions,
storage (Heins et al. 1991, 1994b, Heins and
seasonal availability, or extension of the time
Lange 1992c, Kaczperski et al. 1996).
of availability makes it essential. There are two
stages of plants in bedding plant production and
A major disease problem associated with plug
storage: plugs and finished plants. Generally,
storage is botrytis (Lange et al. 1991a,b, Heins et
finished bedding plants are hardier and can
al. 1992, 1994b, 1995). Maintaining a high RH
withstand greater environmental changes than the
in a cooler decreases the frequency of watering
plug stage of the same plant. However, storage is
but favors the growth of botrytis. The disease is
easier when the plant is in the more compact plug
generally not a problem on pansies but does affect
stage.
many species including impatiens, geraniums, and
petunias. Those crops should be stored under low
RH conditions and irrigated if plugs are stored
Plug Storage
longer than 1 week (Heins et al. 1991).
Most bedding plants are produced as plugs.
Low RH in coolers with significant air velocity
Research on plug storage is mainly limited
causes plugs to dry out quickly. The frequency of
to that published in recent years. Heins et al.
irrigations will vary, depending on the temperature
(1995) found that the duration a species could
and RH of the cooler. Contact between the
be stored without plant death or flowering
foliage and water should be minimized to avoid
delay was influenced by storage temperature
fungal infection. Plugs can be subirrigated with
and irradiance. Plant quality improved with the
clear water as needed during storage because the
addition of light compared to that of plants in dark
plants’ nutritional needs are minimal under low
storage, especially as the duration of storage and
temperatures (Heins and Lange 1992c, Heins
storage temperature increased (Heins and Lange
et al. 1994b). Heins et al. (1994b) recommend
1992a,c,d, Heins and Wallace 1993a,d). The effect
applications of certain fungicide tank mixtures for
of storage on plugs may vary depending on the
controlling botrytis blight and sporulation during
specific storage conditions, age, species, cultivar,
plug storage.
and physiological state of the plugs. The optimum
storage temperatures and maximum storage
durations for selected species are shown in table 1.
Species Cultivars evaluated Optimal storage temperature Maximum weeks storage References
°C °F In the dark In the light
Ageratum ‘Blue Danube’ 7.5 45.0 6 6 Heins et al. 1992
Heins et al. 1994a
Heins et al. 1995
Heins and Lange 1992a
643
Table 1. Optimal storage temperatures and maximum storage durations for plugs of selected bedding plant
644
species and cultivars either in the dark or under a minimum of 5 footcandles of light—continued
Species Cultivars evaluated Optimal storage temperature Maximum weeks storage References
°C °F In the dark In the light
Geranium ‘Pinto Red’ 3.0 37.5 — — Heins et al. 1991
Lange et al. 1991b
New Guniea impatiens ‘Kientzler Agua’ 12.5 55.0 2 3 Heins et al. 1994a
‘Kientzler Anaea’ Heins et al. 1995
‘Kientzler Apollon’
‘Kientzler Celerio’
‘Kientzler Celsia’
‘Kientzler Eurema’
‘Kientzler Marpesia’
‘Kientzler Melissa’
‘Kientzler Octavia’
‘Kientzler Saturnia’
‘Kientzler Sesia’
Table 1. Optimal storage temperatures and maximum storage durations for plugs of selected bedding plant
species and cultivars either in the dark or under a minimum of 5 footcandles of light—continued
Species Cultivars evaluated Optimal storage temperature Maximum weeks storage References
°C °F In the dark In the light
‘Paradise Antigua’
‘Paradise Aruba’
‘Paradise Barbados’
‘Paradise Bora-Bora’
‘Paradise Lanai’
‘Paradise Maui’
‘Paradise Papete’
‘Paradise Samoa’
‘Paradise Tahiti’
‘Paradise Tobago’
‘Paradise Tonga’
‘Paradise Trinidad’
645
5.0-7.5 41.0-45.0 — — Heins & Wallace 1993e
Table 1. Optimal storage temperatures and maximum storage durations for plugs of selected bedding plant
646
species and cultivars either in the dark or under a minimum of 5 footcandles of light—continued
Species Cultivars evaluated Optimal storage temperature Maximum weeks storage References
°C °F In the dark In the light
Salvia ‘Red Hot Sally’ 5.0 41.0 6 6 Heins et al. 1994a
Heins et al. 1995
647
Conifer and Hardwood Seedling Storage The following species may be stored for up to
3 mo at 1 to 4 °C (34 to 40 °F):
Storage of conifer and hardwood forest seedlings
is possible at a low temperature and high RH Norway spruce Sycamore
(Duffield and Eide 1959, Aldhous 1964, Camm Sitka spruce Sweetgum
et al. 1994). Loosely tied bundles of seedlings, Douglas-fir Green ash
as well as containerized seedlings, may be Lodgepole pine Oak
stored in conditions that lower the metabolic Scotch pine Birch
activity of the plants. For most species, however, Loblolly pine Yellow poplar
temperatures should be kept above freezing to Ponderosa pine Hybrid poplar*
avoid injury (Lantz et al. 1989, Camm et al. Western hemlock Eastern cottonwood*
1994). Top and root growth capacity of certain Lawson cypress
seedlings are affected by the cold storage, and
these are dependent on seed source and lifting date * hardwood cuttings
of the seedlings (Jenkinson et al. 1993). A cold-
hardened seedling will store more successfully
and for longer time than a nonhardened seedling. References
Maximum stress resistance occurs in late fall to
early winter. Therefore, lifting dates for seedlings Aldhous, J.R. 1964. Cold storage of forest nursery
being put into cold storage should be delayed as plants: an account of experiments and trials, 1958-
long as possible (Camm et al. 1994). 1963. Forestry (Great Britain) 37:47-63.
Warehouses or sheds can be used for storage Ross, D.S., and R.A. Aldrich. 1976. Production
of seedlings at a variety of temperatures. Operations Analysis. In J.W. Mastalerz,
Refrigerated storage rooms or coolers are also ed., Bedding Plants, 2 edition, pp. 386-397.
used for storing seedlings at cooler temperatures Pennsylvania Flower Growers. University Park,
(1 to 4 °C, 34 to 40 °F). High RH and good air PA.
circulation, as well as daily photoperiod control
if possible, are important factors that influence Barrett, J., et al. 1999. Tips on Growing Bedding
the success of seedling storage (Lantz et al. 1989, Plants. OFA Serv., Inc., Columbus, OH.
Camm et al. 1994).
Borch, K., T. Bouma, J.P. Lynch, and K.M.
Storage of seedlings may be done in polyethylene Brown. 1999. Interactions of ethylene and
bags to facilitate high RH, but spacing between phosphorus nutrition on root growth. Plant Cell
the bags must be enough to allow for adequate air Environ. 22:425-431.
movement in order to prevent fungal pathogens.
Loosely tied bundles of seedlings may be packed Camm, E.L., D.C. Goetze, S.N. Silim, and D.P.
with slightly wet peat surrounding the roots Lavender. 1994. Cold storage of conifer seedlings:
and then wrapped in film-coated paper with an update from the British Columbia perspective.
the tops exposed and placed in a container for Forestry Chronicle 70:311-316.
storage. With prolonged storage (>6 mo), root
growth capacity can decline, as well as lead Duffield, J.W., and R.P. Eide. 1959. Polyethylene
to a disruption of naturally occurring seasonal bag packaging of conifer planting stock in the
progression events. Pacific Northwest. J. Forestry, 57:578-579.
648
Heins, R.D., M.P. Kaczperski, T.F. Wallace Jr., Jenkinson, J. L., J. A. Nelson, and M. E.
et al. 1995. Low-temperature storage of bedding Huddleston. 1993. Improving planting stock
plant plugs. Acta Hort. 396:285-296. quality—The Humboldt experience. General
Technical Report PSW-GTR-143. U.S.
Heins, R.D., and N. Lange. 1992a. Development Department of Agriculture, Forest Service, Pacific
of systems for storage of bedding-plant plugs. Southwest Research Station, Albany, CA.
Bedding Plants Foundation, no. F-056, February,
pp. 1-8. Kaczperski, M.P., A.M. Armitage, and P. M.
Lewis. 1996. Performance of plug-grown
Heins, R.D., and N. Lange. 1992b. How to store geranium seedlings preconditioned with nitrogen
begonia and marigold plugs. Greenhouse Grower, fertilizer or low-temperature storage. HortScience
March, pp. 34-38. 31:361-363.
Heins, R.D., and N. Lange. 1992c. How to store Lange, N., R. Heins, and W. Carlson. 1991a.
salvia and ageratum plugs. Greenhouse Grower, Another look at storing plugs. Greenhouse
February, pp. 76-82. Grower, February, pp. 18-24.
Heins, R.D., and N. Lange. 1992d. One temp can Lange, N., R. Heins, and W. Carlson. 1991b. Store
fit all. Greenhouse Grower, April, pp. 30-36. plugs at low temperatures. Greenhouse Grower,
January, pp. 22-28.
Heins, R.D., N. Lange, and T.F. Wallace Jr.
1992. Low-temperature storage of bedding-plant Lantz, C.W., 1989. A guide to the care and
plugs. In K. Kurata and T. Kozai, eds., Transplant planting of southern pine seedlings. Management
Production Systems, pp. 45-64. Kluwer Academic Bull. R8-MB39, U.S. Department of Agriculture,
Publishers, Dordrecht, The Netherlands. Forest Service, Atlanta, GA.
Heins, R.D., N. Lange, T.F. Wallace Jr., and Nelson, L.J. 1983. Correct conditions extend
W. Carlson. 1994a. Plug Storage. Greenhouse bedding plant marketability. The Great Lakes
Grower. Vegetable Growers News, October, pp. 8-9.
649
Christmas Trees Moisture Status of Cut Trees
Eric Hinesley and Gary Chastagner A Christmas tree is a perishable product that
contains a finite amount of water when cut.
Hinesley is with the Department of Horticultural Postharvest quality and fire safety are closely tied
Science, North Carolina State University, Raleigh, to moisture status. When a tree is cut, it begins
NC; Chastagner is with the Department of Plant to dry. Rate of drying is affected by species as
Pathology, Washington State University, Puyallup, well as environmental conditions (vapor pressure
WA. deficit, temperature, wind) and cold hardiness.
650
The values of V1 and V2 vary by species. In waterings. If this happens, the tree might not
addition, a pressure potential of -3.0 MPa (-30 rehydrate when rewatered.
bar) does not correspond to the same value of MC
in various species. The time required to reach a Water use changes during the display period and
particular MC also varies by species. For example, also varies among species. Compared to other
eastern red cedar and Atlantic white cedar dry species, true firs tend to use large quantities of
very fast when displayed under room conditions, water over extended display periods. For example,
whereas Fraser fir and noble fir dry much slower. a 6-ft (about 2 m) Fraser fir can easily use 4 qt of
The latter species are regarded as long-lasting water per day during the first 5 to 7 days and 2 to
trees, whereas the former have a short shelf-life. 3 qt (about 2 to 3 L) per day thereafter for the next
3 to 4 weeks. In contrast, water use by an eastern
The moisture status of the tree determines its red cedar might decrease noticeably after a week.
ability to rehydrate when recut and displayed in In general, if the tree continues to use a relatively
water. Fresh-cut trees typically have an MC in constant amount of water, it indicates that the
excess of 100%. When a fresh tree is recut and tree is maintaining its initial water status. On the
displayed in water, it typically gains weight, other hand, if there is a marked reduction in water
reaching a MC 5 to 10% above the initial value. consumption, it probably indicates that the tree is
Trees without water gradually lose moisture beginning to dry.
and can readily rehydrate at moisture levels
approaching V2. Beyond that, the degree of
rehydration may decrease; or if rehydration Additives
occurs, there may be adverse changes in quality
(needle abscission, discoloration). For example, Many chemicals and home concoctions have
eastern red cedar rehydrates when V2 = -4.5 to -5.0 been tested in hopes of prolonging the life of cut
MPa (-45 to -50 bar), but not without subsequent Christmas trees. Additives are of little benefit and
abscission of foliage. sometimes produce adverse effects. Additives
can undesirably increase water consumption by
The moisture status during display also varies displayed trees. Because people often use stands
among species. Species that endure for a long time that are too small, increased water consumption
during the display period, such as noble fir and would increase the likelihood of a tree “going dry”
Fraser fir, tend to maintain MC and ψ close to the in the stand. Some additives can induce heavy
initial value for at least 4 weeks. Species that have needle loss. The best tree preservative is plain
a short shelf-life, such as eastern red cedar and water, without additives.
Atlantic white cedar, maintain a high water level
for about 1 wk and then begin to slowly dry even
while displayed in water. This process is reflected Cold Hardiness
by a decrease in ψ (more negative), a decrease in
twig MC, and a reduction in water consumption. Cold-hardened Christmas trees keep better after
harvest and better withstand exposure to low
temperatures. Induction of cold hardiness requires
Water Use photosynthesis, reduced temperatures, and shorter
days. In this context, it will not occur in darkness,
When supplied with water, cut Christmas trees as in a refrigerator. When trees are harvested too
generally consume about 1 qt (about 1 L) of water early, heavy needle loss is possible, even with
per day per inch (2.54 cm) of stem diameter. Thus, proper care. It is not known why cold-hardened
a tree with a 4-in diameter trunk would use about trees keep better than nonhardened trees. Foliar
4 qt (about 4 L) of water per day. The biggest raffinose increases during fall, although the
mistake by consumers is using a stand with too absolute amount is small compared to sucrose.
little capacity, resulting in trees drying up between The increase in raffinose is mostly a response
651
to lower temperatures. The role of raffinose is shoot moth, or pine shoot beetle are frequently
not clear, although in other plants it can reduce prevented from being shipped out of these areas
the ice crystallization temperature in cell sap. unless they have been certified to be free of these
Nonhardened trees also transpire more and thus pests. Many quarantine problems can be avoided
dry faster than hardened trees. by appropriate scouting, trapping, management
practices, and certification programs. Mechanical
Tree species and seed sources also differ in their shakers can be used to remove old dead needles
ability to tolerate exposure to cold temperatures. and certain types of insects. The failure to meet
Coastal types of Douglas-fir are genetically not quarantine requirements can result in entire
as cold-hardy as intermountain types. If coastal shipments being rejected, causing great loss and
types of Douglas-fir are shipped into cold- inconvenience to producers and importers.
weather market areas and not protected from
exposure to cold temperatures, they can exhibit Fumigation. In some instances trees are required
severe needle loss due to cold injury. The level to be treated prior to entry, especially in some
of damage depends on the level of cold hardiness foreign countries. Fumigation is a common
of the trees, the rate of temperature drop, and method used to meet quarantine requirements
the lowest temperatures the trees are exposed for a number of horticultural products. Although
to. People should be aware that tree species, the methyl bromide is one of the most common
environmental conditions prior to harvest, and the materials used to fumigate horticultural products,
environmental conditions that trees are exposed to information on the tolerance of various types of
during transit and on retail lots can have a bearing Christmas trees to methyl bromide is limited.
on postharvest quality. Chastagner (1990) fumigated several species
typically grown in the Pacific Northwest with
methyl bromide at rates up to 6 lb per 1,000 ft3
Diseases and Pests for 2 h at 10 °C (50 °F). Douglas-fir and noble
fir were not damaged, Fraser fir and grand fir
Christmas trees can be damaged by a variety of experienced only slight damage, Scotch pine
diseases and pests. Although trees and branches showed moderate damage, and Shasta fir was
can be killed, most of the damage results in severely damaged. Use of methyl bromide will
cosmetic needle discoloration or loss of needles diminish in the future, and alternatives are needed.
prior to harvest. Little information is available Increasing development of international markets
concerning the direst impact of various diseases will increase the demand for procedures to ensure
and pests on the postharvest keepability of trees. that exported trees are pest-free.
Swiss needle cast on Douglas-fir Christmas trees
can accelerate moisture loss and needle loss when Irradiation. Gamma radiation can be used to
trees are displayed indoors. sterilize or kill insects at all stages of the life
cycle, including larvae and pupae within the
wood. Little information is available for Christmas
Quarantine Issues trees, but balsam fir is sensitive to chronic, low-
level doses of gamma radiation. The cumulative
Live Christmas trees sometimes harbor plant lethal dose (LD50) is about 0.1 kGy over a period
pathogens, insects, and other arthropods. Most are of years. Dormant branches of Fraser fir, when
only an annoyance; but some, if exported to places subjected to single doses of gamma radiation,
that have no natural enemies, could potentially experienced significant needle loss at 0.10 kGy.
cause serious problems. States and countries Massive needle loss occurred within 2 days for
impose various quarantines in an effort to prevent branches that receive higher doses of radiation. In
the introduction or further spread of potentially addition, there was great intertree variation; for
harmful pests. For example, trees grown in areas example, for the 0.1 kGy treatment, needle loss
infested with the gypsy moth, European pine ranged from 5 to 100% for branches displayed
652
for 2 weeks in water (average 42%). Irradiation was any spread of the flame after the sample was
discolored foliage and accelerated drying. removed from the flame, it failed the test. There
is considerable interspecific variation in the MC
If Fraser fir is representative of other Christmas for ignition. In addition, there is often a large
tree species, irradiation does not appear to be a transition zone of MC from the point of initially
viable way to meet insect quarantine requirements. failing the flammability test to the point of flash
Sterilizing insect pests would probably require ignition. For example, Douglas‑fir begins to fail
irradiation doses of 0.5 to 1.0 kGy, and levels the flammability test at about 68% MC, but is
needed to outright kill insects would be higher. totally consumed only when dried to about 30%
These levels of radiation would result in virtually MC.
complete defoliation of Fraser fir within a few
days after exposure. There is a close relationship between twig MC
and water potential (ψ), which also varies among
species. With noble fir, twig MC must reach about
Controlled Atmosphere (CA) and Modified 37% before it begins to fail the flammability test.
Atmosphere (MA) Storage This corresponds to a pressure potential below -6
MPa (-60 bars), well beyond its damage threshold.
Information is limited, but short-term CA or MA Based on this and other postharvest display data,
storage at low temperature (5 °C, 41 °F) appears one can estimate how long it would take for trees
to be of little benefit with Fraser fir. CO2 >5%, as to dry to moisture levels at which they would fail
well as O2 <3%, can lead to increased needle loss. the test. With proper care, winter-hardened noble
The respiration rate at 21 °C (70 °F) is about four fir and Fraser fir can easily be displayed in water
times that at 5 °C (41 °F). for 6 to 8 wk without becoming a fire hazard.
653
Table 1. Postharvest quality ratings for Christmas trees, displayed dry or in water
(Trees are assumed to be cold-hardened.)
Rating
Species Scientific name Displayed—
Dry† Wet
656
Chastagner, G.A., and R.S. Byther. 1984. Impact Hinesley, L.E., and S.M. Blankenship. 1991.
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42(4):4,6,8,10,12,14,16,18-21.
657
Kubiske, M.E., M.E. Abrams, and J.C. Finley.
1990. Keepability of Pennsylvania versus
West Coast grown Douglas-fir Christmas trees:
genotypic variation in relation to subfreezing
temperatures. Northern J. Appl. For. 7:86-89.
658
Cut Flowers and Greens Flower Senescence
659
to the edible crops covered in this handbook. The quality and vase life of many cut flowers can
Nevertheless, understanding these factors allows be improved by pulsing them immediately after
us to develop and implement optimum postharvest harvest with a sugar solution. Pulsing is done
handling technologies. by standing the cut flowers in the solution for
a short period, usually less than 24 h, and often
at low temperature. Typical examples include
Variety tuberose, for which storage life and opening are
dramatically improved; gladiolus, for which
Many commercial cut flowers and cut greens flowers open further up the spike, are bigger, and
are patented cultivars, characterized by specific have a longer vase life; and sweet peas, for which
attributes such as color, form, disease resistance, vase life was improved (Ichimura and Suto 1999).
and size. Sometimes, breeders fail to consider Sugar is also an important part of the bud‑opening
other commercially important attributes. For solution used to open bud‑cut flowers before
example, some of the modern alstroemeria distribution (Kuiper et al. 1995) and as part of the
cultivars have wonderful flowers, but their display vase solution used at the retail and domestic level.
life is short because of rapid leaf yellowing under Potted plants are able to provide their own food
commercial conditions. There is relatively little supply through photosynthesis if they are held in
published information comparing the postharvest adequate light conditions.
life of different ornamental cultivars (Van Der
Meulen-Muisers et al. 1999).
Light
Food Supply
Water Supply
Starch and sugars stored in the stem, leaves,
and petals provide much of the food needed for Cut flowers, especially those with large leaf areas,
cut‑flower opening and maintenance. The levels lose water and wilt very rapidly. They should be
of these carbohydrates are highest when plants stored above 95% RH to minimize water loss,
are grown in high light with proper cultural particularly during long‑term storage. Water loss
management. Carbohydrate levels are, in fact, is dramatically reduced at low temperatures,
generally highest in the late afternoon—after another reason for prompt and efficient cooling
a full day of sunlight. However, flowers are of cut flowers and potted plants. Even after
preferably harvested in the early morning, because flowers have lost considerable water (for example,
temperatures are lower, plant water content is during transportation or storage) they can be fully
higher, and a whole day is available for processing rehydrated using proper techniques. Cut flowers
the cut flowers. will absorb solutions without difficulty providing
660
there is no obstruction to water flow in the stems. water acid (pH ~3.5). Citric acid is commonly
Air embolism, plugging with bacteria, plant debris used as a safe acidulant.
or dirt, and poor water quality reduce solution
uptake (van Doorn 1999).
Water Quality
Air embolism. Air embolisms occur when small
bubbles of air (emboli) are drawn into the stem at Chemicals commonly found in tap water are toxic
the time of cutting. These bubbles cannot move far to some ornamentals. Sodium, present in high
up the stem, so the upward movement of solution concentrations in soft water, is toxic to carnations
to the flower may be restricted. Emboli may be and roses and will cause salt burn (burning of the
removed in many ways; for instance, recutting leaf tips and margins) in potted plants. Fluoride
the stems under water (removing about 2 cm), is very toxic to gaillardia (Rajitha et al. 1999),
ensuring that the solution is acid (pH 3 or 4), gerbera, gladiolus, roses, and freesia. Fluoridated
placing the stems in a vase solution heated to 40 drinking water contains enough flouride (about 1
°C (104 °F; warm, but not hot) or in an ice-cold µL L-1) to damage these flowers.
solution (0 °C), placing the stems in deep (>20cm)
water, or treating the flowers with a detergent
“pulse.” Growth Tropisms
Bacterial plugging. The cut surface of a flower Certain responses of cut flowers to environmental
stem releases the contents of the cut cells stimuli (tropisms) can result in quality loss. Most
(proteins, amino acids, sugars, and minerals) important is geotropism (bending away from
into the vase water. These are ideal food for gravity) and phototropism (bending towards light).
bacteria, yeasts, and fungi, which grow rapidly Geotropism often reduces quality in spike‑flower
in the anaerobic environment of the vase. Slime crops like gladiolus, snapdragon, lisianthus,
produced by the bacteria, and the bacteria and gerbera because the flowers and spike bend
themselves, can obstruct the water‑conducting upward when stored horizontally (Philosoph et al.
system. This problem must be addressed at every 1995). These flowers should be handled upright
step of the postharvest chain by— whenever possible.
662
Specialty flowers such as anthurium, orchid, time for forced‑air cooling ranges from 10 to
ginger, and bird of paradise are packed in 40 min, depending on product and packaging.
various ways to minimize friction damage Flowers should be cooled for three half-cooling
during transport. Frequently, flower heads are times, by which time they are 7/8 cool.
individually protected by paper or polyethylene
sleeves. Cushioning materials such as shredded If the packages are to remain in a cool
paper and paper or wood wool may be placed environment after precooling, vents may be left
between packed flowers to further reduce damage. open to assist removal of the heat of respiration.
Flowers that are to be transported at ambient
temperatures can be packed in polyethylene
Precooling Conditions caskets, foam‑sprayed boxes, or boxes with the
vents resealed. Ice that is used after precooling is
By far the most important part of maintaining the only effective if placed to intercept heat entering
quality of harvested flowers is ensuring that they the carton (that is, it must surround the product),
are cooled as soon as possible after harvest and and care must be taken to ensure that the ice
that optimum temperatures are maintained during does not melt onto flowers or cause chilling
distribution. Most flowers should be held at 0 damage. Precooling of vertical hampers or
to 1 °C (32 to 34 °F). Chilling‑sensitive flowers proconas presents a particular challenge, but can
(anthurium, bird of paradise, ginger, tropical be achieved using a “tunnel” forced-air cooling
orchids) should be held at temperatures above 10 system.
°C (50 °F).
Forced‑air cooling of boxes with end holes or Ethylene Production and Sensitivity
closeable flaps is the most common and effective
method for precooling cut flowers. Cool air is A number of flowers, especially carnations,
sucked or blown through the boxes. Care must be gypsophila, and some rose cultivars, senesce
taken to pack them so that air can flow through the rapidly if exposed to minute concentrations
box and not be blocked by the packing material or of ethylene gas. A number of flowers (such as
flowers. In general, packers use less paper when carnations and sweet peas) produce ethylene
packing flowers for precooling. The half‑cooling as they age, and this endogenous ethylene
663
is involved in the death of the flower. Other Controlled Atmosphere (CA)
flowers, such as snapdragon and delphinium, Considerations
produce little ethylene themselves, but exogenous
ethylene causes flower abscission (or shattering). There have been relatively few reported benefits
Concentrations of ethylene above 100 ppb (nL of CA storage for cut flowers. Any consideration
L-1) in the vicinity of sensitive ornamentals can of the use of CA must follow achievement of
cause damage and therefore should be avoided. proper temperature control and elimination of the
Storage and handling areas should be designed effects of Botrytis during storage. Recommended
not only to minimize contamination of the atmospheres vary from pure nitrogen (daffodils) to
atmosphere with ethylene, but should also have more conventional atmospheres (carnations).
adequate ventilation to remove any ethylene
contamination that may occur. Treatment with
the anionic thiosulfate complex of silver (STS) or Retail Outlet Display Considerations
with 1-methylcyclopropene (1-MCP) inhibits the
deleterious effects of ethylene whether a product The simple way to ensure rapid rehydration of all
of atmospheric contaminant (exogenous) or but the most difficult or desiccated flowers is to
produced by the flower (endogenous) (Macnish et recut the stems and place them in a clean bucket
al. 2000). Finally, refrigerated storage is beneficial of water containing a quality flower preservative
in that both ethylene production and sensitivity are solution in the cooler. The pH of the preservative
reduced greatly when temperatures are low. solution should be below 5. For badly wilted
flowers a rehydration solution may be helpful,
since the sugar in vase preservatives reduces
Optimum Storage Conditions the flow of water in stems. Flowers with woody
branches respond particularly well to low pH (3.5
The vase life of flowers that are stored even is optimal), and some flowers (sunflowers, astilbe)
for a few days is closely correlated with their respond well to a 10-min “pulse” with a 0.02%
respiration during storage. Rapid cooling detergent solution. Flower coolers should be <5
and proper refrigeration are thus essential to °C (41 °F); flowers should be placed in the coolers
maintaining quality and satisfactory vase life when not on display or being used for preparing
of cut flowers and foliage. The recommended arrangements.
conditions for commercial storage of most cut
flowers are 0 to 1 °C (32 to 34 °F) at 95 to 99%
RH. Although flowers are commonly held in Respiration Rates
water for short-term storage, better vase life after
longer storage is achieved by storing the flowers Cut flowers have extremely high rates of
dry. Under these conditions, stable temperatures respiration that increases exponentially with
(to reduce condensation and Botrytis infection) temperature (Cevallos 1998). Various cultivars
and high RH are essential. Flowers for longer- of the same species may have quite different
term storage are typically wrapped in newsprint respiration rates and may respond differently to
(to absorb any condensation) and perforated temperature.
polyethylene (to reduce water loss). Storage
life varies by species but is typically less than 3
weeks.
664
Physiological Disorders Special Considerations
There are relatively few recognized physiological Many special considerations that arise from the
disorders in the postharvest life of cut flowers and unique physiology, handling, and marketing of cut
foliage. “Topple” of tulips, a collapse of the scape, flowers and foliage are addressed in the following
is a disorder associated with low calcium status section for individual crops.
of the flowers. Petal blackening in some red roses
has been suggested also to be associated with
inadequate calcium and perhaps boron nutrition. Guidelines for Individual Crops
665
Lily, Asiatic lily, Oriental lily should be open at time of purchase. Purchase only
Lisianthus, prairie gentian by cultivar name.
Marguerite daisy, Boston daisy
Orchids
Protea, pincushion Grading and Bunching
Rose, spray rose, sweetheart rose
Snapdragon There are no grade standards for alstroemeria,
Statice, German statice but in addition to the common characters of
Stock freedom from damage, stem length, strength, and
Sunflower straightness, it is suggested that the flowers in a
Sweet pea bunch should be uniform. The flower head should
Sweet William be symmetrical, and leaves should be bright green.
Tuberose The minimum acceptable number of florets per
Tulip stem varies with cultivar, but is typically 7 to 10.
Waxflower, Geraldton waxflower
Yellow aster
Ethylene Sensitivity
666
Special Considerations Pretreatments
When recutting, remove the whitish or blanched Pretreatment with STS or MCP prevents the
bottom portion of the stem, if present, for deleterious effects of ethylene.
maximum solution uptake and life. Leaf removal
will reduce vase life if enough flowers are not
present for solution uptake. Since Alstroemeria Storage Conditions
is a member of the Amaryllidaceae, a botanical
family from which many pharmaceutical products Anemones should be stored in a vertical position
are derived, it’s not surprising that some humans at 0 to 1 °C (32 to 34 °F), and may be dry-stored
get allergic dermatitis from this species. for at least 1 week.
Scientific Name and Introduction Flowers are usually packed in standard horizontal
fiberboard boxes.
Anemone spp. Brightly colored in deep reds,
blues, purples, and white, anemones have rather
short stems and are typically a spring flower. New, Special Considerations
improved tetraploid varieties have been introduced
into commerce (Jacob et al. 1997). Anemone is an The anemone’s prominence and beauty in
ancient Greek name meaning windflower, from arrangements is a double-edged sword because
anemos for wind. of the flower’s relatively short vase life. It is
preferable not to use anemones as focal points.
Keep stems wrapped during rehydration to help
Quality Characteristics and Criteria keep them straight. There is no scientific basis for
the practice of piercing a hole through the flower
Flowers should be harvested when the buds are base to extend life. Placing anemones in vases
fully colored and 25 to 50% open, but before with freshly cut daffodils can reduce their life
the petals have expanded and the pollen is shed. because of the harmful juices exuded from the
Purchase when true colors are showing. daffodils.
Anthuriums are commonly packed in moist There are no formal grade standards for asparagus
shredded newsprint or other shredded paper. fern, but fronds should be intact and of uniform
Major damage during transportation is the result length, maturity, and color. Fronds are frequently
of spadices puncturing the spathe of neighboring bunched in groups of 20 and not normally placed
flowers in the pack. Many producers now sheathe in sleeves.
the flowers in small plastic bags and pack the
anthuriums more densely in the box.
Ethylene Sensitivity
Packing
Quality Characteristics and Criteria
Asters are packed in traditional flower boxes,
As with most members of the Asteraceae, hampers, or proconas.
immature flowers (ones harvested too early) will
generally not open properly. Purchase as you
would purchase chrysanthemums, more open Special Considerations
(at least 75% open) than in a bud stage. Avoid
specimens with yellowing leaves as this is an It is very difficult to make broad flower care
indication of improper storage and/or growing recommendations for asters because of the large
conditions. number of species and cultivars. Keep stems and
vase solution clean. It is especially important with
asters to remove leaves that might be in the water
Grading and Bunching since bacteria grow quickly on leaves that are
underwater, contaminating the vase solution and
There are no grade standards as such, apart from leading to early wilting. Treat with a hydrating
the standard quality attributes of stem length, solution, and prepare the preservative solution
foliage quality, uniformity, and freedom from properly to minimize contamination by debris and
defects. Depending on species and cultivar, microorganisms.
bunches may be prepared by stem number (China
aster, for example) or by bunch size (‘Monte
Casino’ aster, for example). Baby’s Breath, Gypsophila
670
is Greek for “gypsum-loving,” in reference to this benefit because stem blockage prevents uptake. Be
species’ good growth performance in high-calcium sure stems are rinsed and recut underwater before
soils. placement in STS.
Gypsophila plants produce flowers on large Store at 0 to 1 °C (32 to 34 °F) in high (90%) RH
panicles whose individual flowers open over a to reduce flower and stem desiccation. Because
considerable time period. Flowering stems are botrytis can be a serious problem, florists should
usually cut 20 to 40 cm (8 to 16 in) long. The ask suppliers (or be prepared themselves) to
degree of maturity at harvest is determined by treat with appropriate fungicides. Stems with
whether the flowers are intended for the fresh about 50% of their flowers open can be kept in a
market or for dried arrangements. Stems are cut preservative solution (200 µL L-1 Physan) at 1 °C
when 50% of the flowers are open if they are to (34 °F) for up to 3 weeks.
be placed in a drying solution immediately or
marketed within 24 h. Stems are cut when 20 to
30% of the flowers are open if they are to be dried Packing
later or held longer than 24 h. Purchase gypsophila
that has plenty of unopened buds and shows no Gypsophila may be packed in horizontal boxes or
signs of water stress, wilting, or disease (brown hampers. Thirty bunches are packed in a standard
florets). box.
Stems are gathered into field bunches using rubber Gypsophila harvested in the bud stage (5% of
bands or ties to secure the cut ends. Bunches from flowers open) can be opened to excellent quality
California are sold as growers’ bunches with 5 to in a bud-opening solution containing 200 µL L-1
25 stems, whereas gypsophila from South America Physan-20 and 5 to 10% sucrose. Flowers should
comes in bunches weighing 300 g. be held at about 20 °C (68 °F) and 50% RH and
with light levels of about 15 mmol m-2 sec-1 PAR
(use cool-white fluorescent lamps). For drying
Ethylene Sensitivity gypsophila, a solution containing 1 part glycerine
to 2 parts water should be used. Cut stems are then
Exposure to ethylene causes wilting of open dried by hanging bunches upside down in a warm
flowers and sleepiness of opening buds. dry environment.
671
the inflorescence a few weeks before harvest. Ethylene Sensitivity
Slender, elongated waxed paper bags are placed
over the expanding bracts a week or more before Strelitzia flowers are insensitive to ethylene and
the orange flowers are ready to emerge. The bags their life is not improved by treatment with STS or
protect the brittle flowers by holding them inside 1-MCP.
or next to the bracts. The bag also helps prevent
Botrytis mold, rain and hail damage, aphid attacks,
and sunburn of the flowers. The specific epithet Pretreatments
“reginae” means “queen.”
Flower longevity can be substantially increased
by pulsing buds or flowers for 24 h (48 h is even
Quality Characteristics and Criteria better) with a solution containing 10% sucrose,
250 µL L-1 8‑hydroxyquinoline citrate (8‑HQC),
Inflorescences bend as they reach maturity, and 150 µL L-1 citrate (Halevy et al. 1978).
assuming a 90° angle to the stem. The first
emerging orange flowers can barely be seen
through the paper bags. The stems are pulled at Storage Conditions
this point. Stems can also be harvested in the tight
bud stage before the first flowers emerge. At this The optimum long-term storage temperature range
stage the inflorescence is swollen, and there is for this species is 6 to 7 °C (43 to 45 °F), which is
a slight orange crack on the upper surface. The different from most other flowers. Storage below
inflorescence is mature at the tight bud stage, this recommended range can result in chilling
and this is the optimum stage for harvest from disorders, the appearance of brown lesions on the
the point of view of ease of handling and flower flowers and bracts, and the failure of the flower
longevity. Flower stems are generally pulled off to open properly. For short-term storage, hold
rather than cut. A side to side pulling motion is the flowers at room temperature or in a tropical
often necessary to loosen the stem at the base of storage room at 12.5 °C (55 °F). Strelitzia flowers
the plant, although the inflorescences can break off harvested in the tight bud stage will open and have
if jerked too vigorously. Make sure flower heads satisfactory vase life after 4 weeks in storage if
are dry at time of purchase. If flowers are wet or pretreated with a fungicide, wrapped to prevent
have excessive nectar exudation upon unpacking, desiccation, and stored at 7 °C (45 °F) and 85 to
then the possibility of subsequent disease 90% RH.
problems is increased.
Packing
Grading and Bunching
Waxed paper bags on each inflorescence and
Strelitzia stems are sorted into at least three grades the paper wrapper on each bunch give ample
according to stem length and inflorescence size. protection from handling injury and desiccation.
For premium grade flowers, the field bags are Because they are very heavy, Strelitzia stems
replaced with new bags. Five stems are firmly should be packed in shallow cartons.
tied together at two points, with the inflorescences
facing in the same direction. The stem ends are
evenly trimmed. A paper wrapper is placed around Special Considerations
the bunched inflorescences to further protect the
flowers. If flowers do not emerge from the spathe
(modified leaf below the flower), the first flower
can be gently eased out by hand and will normally
provide good display life.
672
Bouvardia water containing detergents such as Agral LN or
Nonoxynol-8.5 prior to dry storage prevents early
Scientific Name and Introduction wilting of the flowers (van Doorn et al. 1993).
673
Quality Characteristics and Criteria Packing
Callas should be harvested when the spathe The flowers may be packed horizontally or in
has opened enough that the spadix can be seen. upright hampers or water packs. If they are packed
Flowers harvested more mature will be more horizontally, avoid direct contact with cleats,
susceptible to damage and may have reduced which may damage the fleshy stems. Standard
vase life. The flowers are normally pulled from callas may bend in response to gravity unless held
the rhizome and recut to ensure adequate water at the proper storage temperature.
uptake. The spadix (the thick fleshy inflorescence
inside the showy spathe) should be visible at the
time of purchase. Watch for bruising of the fleshy Special Considerations
stems due to packaging. If cut too tight, flowers
will usually not open properly. The minicallas have fewer postharvest problems
and are generally easier to handle and use because
of their smaller size.
Grading and Bunching
Quality callas have long stems, are uniform in Carnation, Miniature Carnation
maturity and color, and have no defects such as
damage to the spathe or spadix. Both large and Scientific Name and Introduction
mini callas are normally bunched in groups of
10. Minicallas are sometimes sleeved to provide Dianthus caryophyllus. Long one of the most
additional protection. important of the commercial cut flowers, the
carnation has benefited enormously from the
use of STS, which can increase its vase life 2-
Ethylene Sensitivity to 3-fold. The wide range of colors and forms,
especially for miniatures, allows florists and
Calla flowers are not ethylene sensitive, although consumers to use and enjoy them in many ways.
some researchers have shown positive effects of The genus name, Dianthus, derives from the
STS pretreatment. Contrary to popular belief, Greek for “flower of love.” Carnations used to be
callas do not produce significant quantities of called “clove gilly-flowers” in reference to their
ethylene. intense clovelike aroma. Some modern cultivars
are very fragrant and are used to make perfumes.
Carnations can be stored longer than any other
Pretreatments flower and can be opened to high quality flowers
from very tight buds. Miniature carnations are also
Callas do not require any pretreatments. referred to as spray carnations.
Callas should be stored at 0 to 1 °C (32 to 34 °F). The maturity at which carnations are harvested
depends on the proposed marketing procedure.
Star-stage buds are too immature for most
purposes except long-term storage. Buds at the
“paint-brush” stage, with petals straight up, will
open quickly. Flowers for immediate use are
normally harvested with the outer petals between
674
vertical and horizontal. To minimize spread of modified by the addition of a mutation of the
disease, avoid harvesting from plants with obvious ethylene-binding site that makes them insensitive
disease symptoms. Many pickers place cut flowers to ethylene (Bovy et al. 1999).
on top of wires for later collection into bunches.
Flowers collected into canvas slings can be taken
to the shed by mechanical devices ranging from Pretreatments
overhead cables to tractor-hauled trailers designed
to hold slings. Standard carnations ship better and Carnation flowers must be pretreated with 1-MCP
last longer if purchased in the bud stage, while or STS. Research shows that the effectiveness of
miniature carnations should be purchased when 1-MCP is lost within a week at room temperature
at least one flower per stem is open. Fragrant but is retained for extended periods when
cultivars have more consumer appeal. carnations are held at low temperatures. Pulsing
the treated flowers overnight with a preservative
containing 10% sucrose improves flower opening,
Grading and Bunching and quality carnation buds can be opened at room
temperature and under normal room lighting in
Both standard and miniature carnations are graded a solution containing 7% sucrose and 200 µL L-1
by stem strength, stem length, bloom diameter, Physan. The buds should have been treated first
and freedom from defects. Stem strength is with 1-MCP or STS.
determined by holding the stem horizontally
at a point one inch above the minimum length
for the grade. If the deviation of the flower Storage Conditions
head is more than 30° from horizontal (with
the natural curvature down), the flower is Carnations should be stored at 0 to 1 °C (32 to 34
considered defective. Other defects include °F). Bud-harvested flowers perform best in storage
slabsides, bullheads, blown heads, singles, sleepy because they are less sensitive to ethylene than
appearance, splits, discoloration, and damage mature flowers. Flowers or buds for storage should
from insects and diseases. Standard carnations are be of the highest quality and absolutely free of
bunched and tied at the base and at least one other pests and diseases. They should be treated with
place below the flower heads. Instead of different 1-MCP or STS and a fungicide for botrytis control
colored labels, some growers indicate different then packed in a box lined with polyethylene and
grades by color or number of rubber bands on newspaper. Open flowers can be stored 2 to 4
the bunches. Standards for miniature carnation weeks, while bud-cut flowers can be safely stored
bunches vary; a bunch normally contains a up to 4 to 5 weeks. There are methods available
minimum of 30 buds total, at least 7 of which are for storing buds for up to 4 mo.
open. With standard carnations, flower heads may
be alternated (five high and five low) at the top of
the bunch to produce a neat and compact bunch Packing
and reduce the risk of the neck breaking.
Carnations are usually packed in standard
horizontal fiberboard boxes.
Ethylene Sensitivity
675
reduction in vase life is the result; so keep them open, as these flowers will last longer than ones
hydrated when held above 0 to 1 °C (32 to 34 °F). harvested when tighter.
Ethylene Sensitivity
Quality Characteristics and Criteria
Chrysanthemums are not sensitive to ethylene.
Standard chrysanthemums are normally harvested
fully open, or nearly so, and pompons are
harvested with the most mature flowers fully Pretreatments
open. Harvesting too early may result in failure
of the flowers to open. However, chysanthemums Stems should be placed in a rehydration solution
can be harvested as quite tight buds and opened or in water containing a germicide soon after
satisfactorily with simple bud-opening solutions. harvest if they are not to be packed immediately
Bud-cut standards can be harvested when the (van Meeteren and van Gelder 1999). Immersion
inflorescence is about 5 cm (2 in) across or greater in solutions of the cytokinin 6-benzyl adenine
and opened into full-sized flowers. Spray varieties has been shown to be effective in preventing
can be harvested when most of the petals on the premature leaf yellowing in some spray cultivars
most mature flower are still upright. The flowers that are prone to this problem. This treatment is
can be opened after storage or transportation. not yet used commercially. Bud-harvested flowers
can be opened in fresh-flower solutions containing
Stems should be cut (with a knife or shears) 2 to 3% sugar (higher concentrations damage
at least 10 cm (4 in) above the soil line to leaves) at 15 to 20 °C (59 to 68 °F) with 16 h per
avoid taking woody plant tissue. Pinched day of normal room-intensity light. Physan is a
spray chrysanthemums can be pulled from the common, effective germicide, but it discolors the
soil and then cut to correct length. Leaves are stem portion in the solution; therefore, only 3 to
removed from the lower third of the stems. 8 cm (1.2 to 3.2 in) of solution should be used.
Proper rehydration is vital for good vase life of After the buds are open, the injured portion of the
chrysanthemums that have been stored or shipped stem can be removed. Silver nitrate at 25 µL L-1
long distances. Remove chrysanthemum bunches plus citric acid at 75 µL L-1 is very effective but
from the boxes, recut stems to remove about more expensive to use than Physan. Silver nitrate
2.5 cm (1 in), and place in a good rehydration is, however, absorbed into the stem and becomes a
solution. Educate workers and customers to accept lasting germicide throughout the life of the flower.
flowers that are from two-thirds to three-quarters
676
Storage Conditions short vase life that cannot as yet be increased
substantially with standard postharvest treatments.
Chrysanthemums should normally be stored Narcissus is a classical Latin name, from the
at 0 to 1 °C (32 to 34 °F). Bud-cut standard Greek; perhaps, as the origin suggests, an allusion
chrysanthemums harvested when the bud is 1.2 to narcotic properties. It is not clear whether it was
cm (3 in) across can be stored up to 2 weeks, and named after the youth Narcissus in mythology.
1.6-cm (4-in) buds for up to 3 weeks, at 0 to 1
°C (32 to 34 °F). Bud-cut stems that are held in
cold storage beyond the recommended time can Quality Characteristics and Criteria
develop flat-topped flowers. Fully mature blooms
can be stored dry (wrapped in polyethylene) Daffodils are normally harvested at the “goose-
for 3 to 4 weeks at 0 °C (32 °F). Storage at 0 to neck” stage. Jonquils are often harvested at the
1 °C (32 to 34 °F) should not exceed 2 weeks. “one-bell” stage, when only one flower is open on
Yellowing of leaves can occur at 5 °C (41 °F) in the spike. Harvesting is normally done by cutting
the dark, but is less likely at 1 °C (34 °F). the flower from the foliage and bulb, although the
whole plant may be removed and the bulb and
leaves then cut from the flower spike. Flowers
Packing should be purchased in the pencil to goose-neck
stages. These terms refer to flower position
Chrysanthemums are normally packed in standard relative to the stem; pencil being straight up and
horizontal fiberboard boxes. Standards are packed gooseneck bent downwards to about a 45° angle.
individually, and a layer of wax paper often
separates each row of flowers.
Grading and Bunching
677
Pretreatments Delphinium, Larkspur
Pretreatment with 1-MCP or STS can help extend Scientific Name and Introduction
vase life of flowers that are likely to be exposed to
ethylene. Delphinium, Consolida spp. The tall spikes of
delphinium and the smaller spikes of larkspur are
important accent flowers with colors ranging from
Storage Conditions white through pink, purple, and blue.
Ethylene Sensitivity
Special Considerations
Delphiniums are very sensitive to ethylene, which
Daffodils exude a gelatinous (slimy) substance causes rapid loss of all of the flowers on the spike.
that, when transferred through a common holding
solution to other flowers like tulips and anemone,
can result in premature death for the other species Pretreatments
(van Doorn 1998). Therefore, place freshly cut
or recut flowers into a separate holding bucket Flower spikes should be pretreated with 1-MCP
for a few hours. Later they can be placed with or STS to extend their vase life and protect them
other flowers and used (even recut if required) from exposure to ethylene. Because flowers are
in arrangements without affecting the life of the on spikes and are therefore at different maturity
other flowers. stages at the time of anti-ethylene treatment, STS
may be more effective than 1-MCP.
678
Storage Conditions Grading and Bunching
Delphinium and larkspur should be stored at 0 to There are no formal grade standards for
1 °C (32 to 34 °F). For longer term storage, they Chamaedorea, but uniformity, size, color, and
should be wrapped in perforated polyethylene to absence of defects are important criteria of quality.
reduce water loss. Bunches of emerald palm contain 25 stems.
These flowers are often packed in hampers with Exposure to ethylene has no deleterious effects on
or without water, but they may also be packed in Chamaedorea fronds.
standard horizontal boxes. They are somewhat
sensitive to gravity, so horizontally packed flowers
should be precooled and maintained at proper Pretreatments
holding temperatures.
No pretreatments are recommended for
Chamaedorea fronds.
Special Considerations
Chamaedorea palms are harvested in the wild as Early death of the fronds, drying, and inrolling of
well as being produced in plantations. Fronds are individual leaves (pinnae) result from water stress.
harvested when fully expanded, mature, and dark Make sure that stems are recut before arranging
green. Fronds of Chamaedorea should be dark them as this can quadruple their life. The species
green, clean, and uniform. Avoid fronds whose is chill sensitive, so hold at proper temperatures.
leaf tips show marginal necrosis or dead areas and
fronds that are beginning to turn yellow.
679
Eucalyptus, Silver Dollar Tree Storage Conditions
Quality Characteristics and Criteria Handle this species with gloves to prevent hands
from becoming sticky. Eucalyptus is native
As with other foliage, eucalyptus performs better to areas like Australia and Tasmania, but over
in the vase if leaves are mature. Branches are 200 species have been introduced elsewhere.
harvested to provide long stems but leave growing As a result, this species predominates in certain
points behind for development of new branches. woodlands in California. Many florists believe
Stem tips should not be wilted when purchased. eucalyptus gives off a lot of ethylene because
of its strong fragrance. In fact, most species
and cultivars do not produce excessive amounts
Grading and Bunching of ethylene gas, although some will produce
potentially detrimental quantities of ethylene if
Quality foliage is bright blue-green in color, has they become water-stressed. Hence, make sure
undamaged leaves, and is uniform in length. this species is properly hydrated. Eucalyptus
Eucalyptus branches are sold in grower bunches can be treated with various colored glycerin-
by weight, usually as 454 g (1 lb) bunches. based solutions, which result in preserved
specimens. Australian and Israeli researchers are
investigating the possible use of eucalyptus as
Ethylene Sensitivity flowering branches, and we may expect to see
this interesting item increasing in the trade in the
Eucalyptus branches are not sensitive to ethylene, future.
but if the foliage is poorly handled, warm, and
water-stressed, it can produce concentrations of
ethylene that could damage ethylene-sensitive Fir, Spruce, Pine
flowers that have not been treated with 1-MCP or
STS. Scientific Name and Introduction
680
Quality Characteristics and Criteria Special Considerations
Branches should be harvested when tips and Antitranspirant dips have not been shown to
needles are mature. Soft green growth is more reduce water stress or to extend user life. Despite
likely to wilt in the vase. Branches should be their strong aroma, these foliage materials have
mature, with uniform dark green foliage. Avoid not been demonstrated to produce ethylene. If they
bunches with fungal growth or whose needles are are infected by fungi, it is possible that they may
falling off. produce ethylene, but otherwise they are safe to
store with ethylene-sensitive flowers.
Because of the low value and relative hardiness Grading and Bunching
of these materials they are usually packed loose
in horizontal fiberboard boxes without any There are no standard grades for freesias, but they
protection. may be graded according to maturity, number of
flowers per stem, and the length of stem. Quality
freesias have at least seven florets per spike and
681
have long, straight stems. Flowers are sold in Gerbera, Transvaal Daisy
bunches of 10 stems, usually of the same color.
Scientific Name and Introduction
682
Ethylene Sensitivity are packed horizontally into a fiberboard box in
such a way that the flowers are facing upward and
Gerberas are unaffected by exposure to ethylene. easily seen while the stems are underneath the
flower cards.
Pretreatments
Special Considerations
Present industry practice is to place cut gerbera
stems in a 40 µL L-1 sodium hypochlorite solution Stem bending is primarily in response to gravity
immediately after harvest to improve vase life. and is greatly reduced if flowers are held at the
A rapid pulse treatment with 100 µL L-1 silver proper storage temperature. One of the major
nitrate is sufficient to greatly alleviate postharvest problems in postharvest handling of these flowers
problems with gerbera cultivars that are relatively is their tendency to “conk”: the stem folds 10 to
short-lived. The silver nitrate presumably guards 15 cm (4 to 6 in) below the flower head, resulting
against bacterial contamination of the stem and in an unmarketable flower. This bending has been
vase water. After the dip, rinse the flowers in variously attributed to harvesting of the flower
good-quality water. This treatment causes only before the stem has hardened sufficiently and to
minimum phytotoxicity (brown damage to the microbial plugging of the stem and subsequent
stem). The use of 6% sugar plus 200 µL L-1 water stress. The tendency to conk varies with
8-HQC as a preservative has shown to be of some variety and also varies throughout the year for any
benefit but can cause stem elongation during given variety. Be sure to enhance water uptake
storage and may reduce overall flower quality. by keeping holding solutions and buckets clean
and including hypochlorite in the water. Since
more water is lost through the flower stem (scape)
Storage Conditions than through the flower petals, the scapes should
be handled with as much care as the flowers
Gerberas should be stored at 0 to 1 °C (32 to themselves. Hang flower heads through a meshed
34 °F); the widely-held opinion that gerberas support or shipping tray when first hydrating to
are sensitive to chilling injury has not been keep stems straight.
scientifically substantiated. Generally, gerberas
should not be stored longer than 1 week; even this
short storage period can reduce subsequent vase Ginger, Shell Ginger, Torch Ginger
life.
Scientific Name and Introduction
683
Quality Characteristics and Criteria Special Considerations
No specific maturity standards have been Their large size makes gingers difficult to manage.
developed for these flowers. They are normally Since insects sometimes make the trip from
harvested when all of the flowers on the spike grower to wholesaler to retailer, make sure flowers
are open. Make sure flowers do not exhibit chill are inspected and any insects removed.
damage symptoms such as off-colored (grayish/
bluish) blooms.
Gladiolus, Glad
There are no specific grade standards for gingers. Gladiolus cvs., hybrids. Still an important
Proper maturity and freedom from flower and commercial cut flower despite a substantial
foliage defects would be important indicators of decline in production in recent years, gladiolus
quality. Gingers are large flowers and are therefore responds well to proper postharvest management.
normally packed individually. Some species may The smaller flowered and “butterfly” cultivars, as
be individually sleeved to protect the delicate well as modern standards in a variety of colors
petals. and forms, have helped transform this often
stereotypic funeral flower into a contemporary
favorite that can be an important accent flower
Ethylene Sensitivity in arrangements. Gladiolus is Latin for “small
sword,” in reference to the sword-shaped
These flowers do not appear to be particularly leaves. Modern day gladioli are the results of
sensitive to ethylene. hybridization programs that commenced in
Belgium around 1841 using South African species.
Pretreatments
Quality Characteristics and Criteria
There is no evidence that pretreatments provide
any benefit to ginger flowers. Normal harvest is at the stage when the bottom
two or three florets on the spike are showing
color. For long-distance transportation, an even
Storage Conditions earlier harvest stage can be recommended if it
is combined with sugar pulsing to ensure proper
Store at 12.5 to 15 °C (55 to 59 °F). Gingers are opening of the flowers at their destination. Local
chilling sensitive, so they must be held at warmer market flowers are cut when the first floret is
temperatures. open. Harvesting is carried out so as to leave as
many leaves on the plant as possible. A knife is
run down between the leaves with the back of
Packing the knife down. When the knife blade is as low
as the cutter believes it should go, it is pulled
Gingers are packed flat in standard or insulated upward and out, severing the stem, which can
fiberboard boxes. then be pulled out of the leaves. It is possible to
open almost all florets on flower spikes if they
are harvested in the green bud stage and handled
properly. However, it is recommended that
color be visible in one to three florets at time of
purchase to help ensure that most florets will open.
684
Grading and Bunching allowing gladioli to be packed in the standard
horizontal flower box. For longer storage, gladioli
Gladioli, like most spike-type flowers, are are best stored upright at the lowest safe storage
very sensitive to gravity and will always tend temperature.
to grow away from the ground, particularly at
warm temperatures. This can result in permanent
deformation of the upper part of the spike and Packing
consequent reduction of flower quality. To avoid
deformation, gladioli should be held upright Gladioli are traditionally packed in tall “glad
throughout postharvest procedures. This rule may hampers” clearly marked for upright stacking.
be relaxed only while the flowers are held at low Since the advent of precooling, some shippers
temperature during storage and transportation. have packed gladioli in normal flower boxes.
Quality factors for gladiolus include stem This practice is fairly safe if the flowers will
straightness and strength, freedom from damage remain refrigerated throughout the marketing
and disease, and maturity. The flowers are chain and will be removed from the box on
bunched by color and maturity in groups of 10. arrival. Excessive moisture on the foliage should
be avoided so as to minimize the risk of Botrytis
infection.
Ethylene Sensitivity
685
Quality Characteristics and Criteria Packing
Heliconia are normally harvested fully mature: Heliconias are normally packed in horizontal
The flowers will not open past the stage at which fiberboard boxes.
they are harvested. While flowers last longer if
the bracts are less open compared to more open,
they generally do not open further after harvest Special Considerations
and that may reduce their visual appeal. Therefore,
the openness of the flower at purchase often is Heliconias most often die early due to poor water
the most it ever will open. Consumer life varies uptake. They can last for up to 2 weeks in plain
greatly among species and cultivars; thus, learn tap water if the water is free of microorganisms.
species and cultivar differences. Larger diameter and longer stemmed specimens
last longer. Since insects sometimes make the trip
from grower to retailer, make sure flowers are
Grading and Bunching inspected and any insects removed.
686
Ethylene Sensitivity Huckleberry
Exposure to ethylene results in loss of berries and Scientific Name and Introduction
leaves and is a common problem in holly handled
through mass-market outlets. Vaccinium ovatum. Huckleberry is native to the
West Coast in areas from northern California
to British Columbia. Vaccinium is the ancient
Pretreatments Latin name for blueberry, and the specific epithet
ovatum refers to the oval leaf shape of this species.
Because of its ethylene sensitivity, holly should
be pretreated with STS or 1-MCP, which prevents
bud and leaf loss during marketing (Joyce et al. Quality Characteristics and Criteria
1990).
Harvest branches when fully mature, without soft
tips. This species is very long lasting with few
Storage Conditions postharvest problems. Thus, if it is of good quality
at the time of purchase, it should perform well.
Holly should be stored at 0 to 1 °C (32 to 34 °F)
and may even be held for longer periods at lower
but nonfreezing temperatures. Grading and Bunching
N/A.
Packing
Special Considerations
Storage Conditions
There are major differences among holly types
in their sensitivity to ethylene. For example, Huckleberry can be stored for an extended period
‘Burford’ is essentially insensitive to ethylene, by enclosing precooled bunches in a plastic vapor
while Chinese and English types are very barrier and holding them near 0 °C (32 °F).
sensitive. Therefore, know the type of holly being
marketed. Some producers will dip holly into
various solutions in an attempt to reduce fruit Special Considerations
and leaf fall or to package the product in such a
way as to extend life. However, at wholesale or Watch for excessive storage as depicted by
retail levels there is not presently a product to fungal growth (fuzziness) and watersoaked or
retard fruit loss other than STS. Treating with STS discolored leaves. The woody stems sometimes
does reduce fruit and leaf fall. Do not pregreen make huckleberry difficult to recut. Recutting this
arrangements with holly, as it will not last. species is less critical than for other floral crops.
687
Iris, Fleur-de-Lis Ethylene Sensitivity
Scientific Name and Introduction Iris are not affected by exposure to ethylene.
Leatherleaf Fern
Grading and Bunching
Scientific Name and Introduction
There are no formal grade standards for iris
flowers. Flowers should be uniform in variety, Rumohra adiantiformis. By far the most popular
color, and maturity. Foliage should be relatively cut foliage for use in arrangements, with
undamaged and free from disease. Stems should year-round availability and good display life,
be strong and straight. Flowers are normally leatherleaf fern is grown in shade-houses under
bunched in groups of 10 and tied with rubber subtropical conditions. The specific epithet
bands or twist-ties. adiantiformis indicates the similarity to the fronds
of Adiantum, the maidenhair fern. The Greek
688
adianton means unwettable—a reference to the The brown bumps (sori or fruit-dots) found on
fact that fern fronds shed water. It is probably the the back of some fronds have not been reported to
most commonly used floral green. reduce life.
1 to 6 °C (34 to 42.8 °F) Quality foliage has uniform mature green leaves
with no damage, defects, or disease. Salal is
usually sold 20 stems per bunch.
Packing
689
large bins lined with plastic to reduce water loss. Grading and Bunching
Even though the stems are woody, salal is adapted Liatris flowers are not affected by exposure to
to standard florists’ procedures for rehydration and ethylene.
use in arrangements.
Pretreatments
Liatris, Gay Feather
Pulsing with preservative containing additional
Scientific Name and Introduction sugar (10 to 20%; about 100 g of sugar per quart
or liter of water) will improve opening of tight-cut
Liatris pycnostachya, L. spicata. Native to prairies flowers (Han 1992).
of North America, liatris was developed as a cut
flower in Israel. The bright purple spikes provide
interesting texture and line in arrangements, and Storage Conditions
can open fully if properly treated after harvest.
Members of the Asteraceae (chrysanthemum or Store liatris at 0 to 1 °C (32 to 34 °F).
aster family), these species are also unusual in that
flowers open from the top of the stem downwards;
delphinium, gladiolus, snapdragon, and most Packing
other spiked-type flowers open from the bottom
up. L. spicata is the taller of the two species. The Liatris are normally packed in horizontal
specific epithet pycnostachya means thick-spiked fiberboard boxes but may also be packed in
in reference to flowers, while spicata means spike. hampers.
Liatris spikes should be harvested with no more Be careful of fungal problems such as Botrytis
than 25% to 33% of the flowers in the spike open. (especially for flowers grown outdoors) as well
They may be harvested with only the top buds as water stress. Leaf yellowing and reduced life
showing color and will open fully if provided with are common when these problems exist and are
an effective preservative. However, if preservative not easily controlled. Using preservative solution
solution is properly used, flowers can be harvested helps open more flowers per stem but doesn’t
with no color showing and subsequently opened. make individual flowers last longer.
690
Lily of the Nile, Agapanthus the decrease in vase life due to even short-term
storage and is of no apparent benefit to flowers
Scientific Name and Introduction harvested immature.
Ethylene exposure results in loss of florets from Lilium spp. Lilies have long been popular as
agapanthus. garden flowers, prized for their stately beauty;
and potted white lilies are a tradition in countries
that celebrate Easter or Christmas. The brightly
Pretreatments colored flowers of hybrid lily cultivars have
become increasingly popular as cut flowers and
The vase life of freshly cut agapanthus flowers have excellent vase life, especially if pretreated
was shown to be significantly extended by to prevent effects of ethylene. Buds open well if
pretreatment with a 3 h pulse in 4 mM STS, provided with preservative. “Lilium” is from the
followed by spraying (to runoff) with 30 µL L-1 Greek word lirion, used in the naming of certain
NAA, followed by a 48 h pulse in a solution subdivisions of the genus.
containing 10% sucrose, 300 µL L-1 citric acid,
and 300 µL L-1 Physan-20 (Mor et al. 1984).
This pretreatment is not sufficient to counteract
691
Quality Characteristics and Criteria dry at 1 °C (34 °F). Flowers should be properly
precooled, and they must be packaged so that
Lilies are normally cut when one or two buds water loss during storage is minimized (wrap
are just beginning to “crack.” Tight-bud-stage precooled flowers in polyethylene film). However,
flowers take some time to open, and petals of open some problems with leaf browning or yellowing
flowers are likely to be damaged during transport. can occur even after 2 weeks storage.
Most species and cultivars should be purchased
with color showing on at least one flower. Avoid
bunches with a number of open flowers, since they Packing
probably have been held at warmer temperatures
during marketing. Lilies are normally packed in horizontal fiberboard
boxes.
692
Grading and Bunching Marguerite Daisy, Boston Daisy
There are no grade standards for lisianthus, but Scientific Name and Introduction
obvious leaf miners and damage to the flower
are quality defects. Bunches consist of 10 flower Argyranthemum frutescens. The white or yellow
stems. flowers of Margeurite daisies are produced year-
round outdoors in frost-free areas of California.
Borne on a perennial bush, flowers have long been
Ethylene Sensitivity an inexpensive staple for florists, often dip-dyed
to provide different colors for special holiday
Lisianthus is slightly sensitive to ethylene. occasions. Their postharvest life is relatively long
Exposure of mature flowers to ethylene will but often limited by wilting or yellowing of the
decrease their ultimate vase life, but the effect is foliage. The specific epithet frutescens means
relatively slight and does not warrant treatment bushy.
with 1-MCP or STS.
693
Ethylene Sensitivity and excessive or ineffective preservative solution.
White flowers often are submerged in dye to get
Margeurite daisies, like other members of the pink, green, red, or blue colors.
Asteraceae, are not affected by exposure to
moderate concentrations of ethylene.
Orchids
Research has shown improved performance with Cattleya, Cymbidium cvs., and hybrids.
flowers that are pulsed overnight at 20 °C (68 °F) Additional genera in the plant family Orchidaceae
with 25 µL L-1 silver nitrate and 0.5% sucrose are Dendrobium, Phalaenopsis, Vanda, and
before storage or transport. Sucrose concentrations Paphiopedilum. In addition to their exotic forms
above 0.5% can accelerate leaf yellowing and and colors, one of the principal attractions of
cause leaf injury. cut orchid flowers is their outstanding longevity.
Even out of water, an orchid flower will last for an
evening in a corsage. Spikes of cymbidiums will
Storage Conditions often last a month in a vase.
Water in which Margeurites are held often There are no grade standards for orchids. Freedom
develops a bad odor. Keep solutions fresh and from defects is a primary measure of quality.
buckets clean. Remove dirt and debris from stems
prior to cutting them. Preservative solutions
offer varying degrees of benefit depending on Ethylene Sensitivity
brand. Avoid preservative solutions containing
8-hydroxyquinoline citrate or sulfate (generally Some genera such, as Cymbidium and
ones that turn the water slightly yellow). Phalaenopsis, are very sensitive to ethylene;
Lower foliage often turns yellow, which can be others, such as Dendrobium, are less sensitive.
accelerated by improper storage or precooling
694
Pretreatments Protea, Pincushion
Special Considerations
Ethylene Sensitivity
Only some species and cultivars are ethylene
sensitive, which explains why anti-ethylene Neither foliage nor flowers of the proteas are
treatments like STS and 1-MCP work only some affected by exposure to ethylene.
of the time. The two most common ethylene-
induced symptoms are flower discoloration and
premature wilting and flower fall. Demand has Pretreatments
increased for this species prepared as a corsage
and sold through mass market outlets at Easter and Species that are susceptible to leaf blackening may
Mothers’ Day. When sold for corsages the use of be pretreated by pulsing overnight at 20 to 25 °C
water picks filled with fresh-flower solution (not (68 to 77 °F) with 5% sugar (sucrose or glucose)
plain tap water) is beneficial. (Dai and Paull 1995). Higher concentrations may
cause leaf blackening.
695
Storage Conditions often results in the symptom called “bent neck”
in which the flower neck wilts and the bud fails
Flowers and foliage from the Proteaceae should to open. We also found that many commercial
be stored at 0 to 1 °C (32 to 34 °F). Rapid cultivars are quite sensitive to ethylene gas. The
precooling and maintenance of the proper storage cut-flower industry has an important stake in
temperature are important tools in preventing leaf overcoming the poor postharvest reputation of cut
blackening, a common postharvest problem in roses. All that is required is proper postharvest
proteas. Care must be taken to ensure that there care for those cultivars susceptible to bent neck
is no condensation or free water on leaves during and appropriate pretreatment of those that are
storage as this greatly increases the incidence of sensitive to ethylene, especially if they are to be
blackening. sold in supermarkets or other ethylene-polluted
areas.
Packing
Quality Characteristics and Criteria
Proteas are normally packed in horizontal
fiberboard boxes. Roses are harvested at different levels of maturity,
depending on marketing and cultivar. For long-
distance transport or storage, roses should usually
Special Considerations be harvested with some of the sepals reflexed.
Flowers harvested before the sepals reflex may fail
Leaves turn black due to lack of carbohydrate to open or may be more susceptible to bent neck.
(food) and warm temperatures. Use preservative Fast-opening roses, like some yellows and whites,
solutions and proper low temperature management should be harvested just before the sepals start to
to prevent leaf blackening. In addition, this separate from the bud. The marketing life of roses
disorder can be reduced if the flowers are held harvested later will be reduced unless extra care is
under lighted conditions. Leaf blackening is not taken with their postharvest handling.
due to low temperature (chilling disorder), nor
is it due to poor water relations. Many species Harvesting is most convenient using shears
and cultivars can be easily dried or preserved by provided with auxiliary jaws to hold the bloom
just allowing them to dry under warm, low-RH after harvest. The cut is normally made so as to
conditions. leave two five-foliate leaves below the cut. When
stem length is an important consideration, the cut
may be made below. Roses should be purchased
Rose, Spray Rose, Sweetheart Rose and sold by cultivar name. Avoid blooms that are
already open; flowers should normally have some
Scientific Name and Introduction or all of their sepals (the green protective leaves at
the base of the flower) folded back, but the petals
Rosa cvs., hybrids. The rose undoubtedly remains should not have started unfolding. Brown spots or
the queen of the cut flowers. The historical patches on the outer petals may be an indication of
association of this flower with romance and beauty Botrytis infection.
ensures that roses will continue to be a highly
desired cut flower in the future. Properly handled,
most of the commercial cut roses will easily last Grading and Bunching
in the vase for 10 days. Unfortunately, many
consumers consider roses to have a very short Objective grading is based on stem length;
vase life. This is partly because poor water uptake subjective grading is based on flower maturity,
by certain cultivars of purchased roses all too stem straightness, stem caliper, and quality of
696
flower and foliage. Defects on the outer “guard” Packing
petals are not normally a cause for down-grading,
because these petals are removed by the retail Rose bunches are routinely sleeved in plastic,
florist. Leaves and thorns may be removed waxed paper, or soft corrugated card sleeves. The
manually or mechanically if desired. This “spiral” bunch used by many off-shore producers
operation has little effect on vase life if flowers are increases the difficulty of precooling the flowers,
placed in an effective preservative. The number as well as the opportunity for condensation
of stems per bunch and bunch pattern (single collecting on the outer petals. Botrytis infection is
layer, staggered two-layer) depend on market a common result of the presence of free moisture
preferences. on the petals of cut roses.
Some cultivars are ethylene sensitive. Treat Removal of those leaves and thorns below the
with 1-MCP or STS if they are being distributed water line should not reduce vase life if stems are
through the mass markets, especially if being placed into a preservative solution. The fungus
shipped through distribution centers, and also treat Botrytis represents a major problem for roses.
to prevent the effects of the ethylene prior to dry Symptoms of Botrytis infection include brown
storage. blotches on petals and gray, fuzzy growth on
leaves, stems, or flowers. Postharvest fungicide
dips can be helpful—use only registered products
Pretreatments according to label instructions. Petal blackening
on some red cultivars is due to growing conditions
Roses should be pretreated with 1-MCP or STS and cannot be corrected at wholesale or retail
to prevent the effects of ethylene, especially if levels.
they will be sold through a supermarket. Sugar-
containing pretreatments are not particularly
useful for roses. Rehydrate after cutting, Snapdragon
storage, and on arrival at the retail outlet with a
rehydration solution. Commercial rehydration Scientific Name and Introduction
solutions are effective, as is clean water containing
50 µL L-1 hypochlorite, preferably below pH 5.0. Antirrhinum majus cvs. The many pastel flower
This solution has proved safe and is inexpensive, colors of the tall spikes of snapdragons allow
so the buckets can be filled to the desired 20 to 30 florists and consumers innumerable design
cm (8 to 12 in) depth (Reid et al. 1996). options. Snapdragons used to be considered very
sensitive to ethylene, but the newer cultivars have
been selected for ethylene resistance, and ethylene
Storage Conditions normally causes a problem only when present in
moderate concentrations. Flower drop (shattering)
Roses should be stored dry at 0 to 1 °C (32 to 34 occurs in 24 h if ethylene is present in air at
°F). Roses intended for long-term storage should ≥0.5 µL L-1. Antirrhinum is Greek for “like” and
be packed in polyethylene-lined cartons and “nose,” in reference to the flower shape.
precooled. They may be held for up to 2 weeks in
dry storage if the temperature is maintained close
to the freezing point.
697
Quality Characteristics and Criteria the growth regulator n-dimethylamino succinamic
acid increases flower quality and also counteracts
For local sales, snapdragons are typically the excess spike length that sometimes results
harvested when flowers on the lower half to two- from placing snapdragons in 8-HQC and sucrose.
thirds of the spike are open. A less mature stage Pulse treatments with naphthylpthalamic acid or
is desirable for shipping and short-term storage. calcium antagonists (Philosoph-Hadas et al. 1996)
Purchase those cultivars that are less sensitive can reduce geotropic curvature that results from
to ethylene. At least two to five florets per stem horizontal storage at warmer temperatures.
should be open. Avoid flowers with excessive stem
bending and yellowing foliage, which indicate
poor temperature management after harvest. Storage Conditions
698
preservative solutions. The many pastel flower Ethylene Sensitivity
colors allow florists and consumers innumerable
options. The flower can be made to snap shut after Statice flowers are sensitive to ethylene, which
separating and releasing the two-lipped corolla causes accelerated wilting. In standard statice,
(fused petals). When grown as a garden plant, this effect is not apparent since the “flowers” are
treat the flowers as an annual, although many will papery bracts surrounding the true flowers. In
respond as a perennial, depending on location and hybrid statice cultivars, effective treatments to
cultivar. inhibit action of ethylene improve flower quality
and vase life.
699
Stock Storage Conditions
Scientific Name and Introduction Stock should be stored at 0 to 1 °C (32 to 33.8 °F).
Exposure to ethylene results in water-soaking of Sunflowers are normally harvested when the
the petals, accelerated senescence of the florets, petals (the outer flowers or ligules) have unfolded
and epinasty (downward curvature) of the leaves. and are at least vertical. For local market, flowers
are harvested with ligules fully expanded and
horizontal. No yellow, wilted leaves should be
Pretreatments present. Storage life is often determined more
by leaf yellowing or desiccation than by flower
To prevent deleterious effects of ethylene, stock problems.
should be pretreated with 1-MCP or STS.
700
Grading and Bunching Sweet Pea
Quality sunflowers are of uniform maturity, are Scientific Name and Introduction
free from defects, and have straight stems and
good quality foliage. Small-flowered cultivars Lathyrus odoratus. Once important cut flowers,
may be bunched in groups of 10 or 12, and large- prized for their aroma and range of colors, sweet
flowered types are normally packed individually. peas benefit substantially from anti-ethylene
pretreatments. Combined with a sugar pulse,
treatment with STS or 1-MCP enables these
Ethylene Sensitivity delicate flowers to be harvested at an earlier stage
when the flowers are less susceptible to damage
Prolonged exposure of sunflowers to low and can give as much as 1 week of display life.
concentrations of ethylene results in abscission of
ligules.
Quality Characteristics and Criteria
Packing
Grading and Bunching
Sunflowers are normally packed in standard
horizontal flower boxes. There are no grade standards for sweet peas, but
quality flowers have long, straight stems and at
least five buds on each spike. Flowers can be
Special Considerations bunched by color, or in mixed colors, in bunches
of 10.
Sunflowers are also somewhat sensitive to gravity.
If held horizontally at warmer temperatures, the
flower heads will be permanently bent down; so it Ethylene Sensitivity
is important to maintain cool temperatures during
transport and storage. Exposure to ethylene results in accelerated wilting
of petals, abscission of flowers, and failure of
developing buds to open.
701
Pretreatments be harvested with the outer ring of flowers open.
Flowers should have at least the outer whorl
Proper pretreatment greatly improves the vase of florets open. Avoid flowers with withered or
life of sweet peas. Flowers should be treated with sleepy florets, as this indicates ethylene-induced
1-MCP or STS (Sexton et al. 1995), then placed in problems.
a preservative solution containing 4% sucrose at
20 °C (68 °F) overnight.
Grading and Bunching
Dianthus barbatus. A close relative of the Sweet william flowers are normally packed in
carnation, normally grown in the field, sweet horizontal fiberboard boxes.
william flowers are borne on a short-stemmed
inflorescence. Colors range from white through
intense red and purple and provide strong accents Special Considerations
in an arrangement. The specific epithet barbatus
means bearded or barbed in reference to the As with many flowers grown in the field, fungal
beardlike growth emerging from the petals. infections resulting from the wet foliage and
flower conditions sometimes experienced at
harvest can be a problem. Make sure flowers are
Quality Characteristics and Criteria rapidly unpacked and aerated to reduce possible
disease spread.
Flowers in the sweet william inflorescence
continue developing after harvest, and they should
702
Tuberose Pretreatments
Ethylene Sensitivity
Tulip
Floret opening is reduced in spikes exposed to
high concentrations of ethylene; but this effect is Scientific Name and Introduction
probably not normally of commercial significance,
so 1-MCP or STS treatments are not warranted for Tulipa cvs., hybrids. Tulips, one of the classical
tuberose. cut flowers, were the source of tremendous interest
when they were first brought to Holland from the
Mediterranean countries where they are native.
703
The most common species, Tulipa gesnerana, was Control of RH and proper rehydration following
named after C. Gesner, a botanist who lived from storage can minimize the problem.
1516 to 1565.
Packing
Quality Characteristics and Criteria
Tulips may be packed in hampers or in regular
The entire tulip plant, with bulb attached, is fiberboard flower boxes. Stems and blooms should
harvested when the tepals show 50% color. It is be securely wrapped to prevent bruising and
preferable to harvest in the early morning when breakage. Tulips packed horizontally must be held
temperatures are lower and to cool the harvested at proper temperature of 0 to 1 °C (32 to 34 °F) to
flowers immediately. Purchase when flower color avoid gravity-induced bending.
is just visible and only by cultivar name since
postharvest characteristics vary greatly.
Special Considerations
704
at the point,” in reference to the leaves which are Storage Conditions
hooked at their ends. The crushed leaves give a
pleasing citrus aroma. Store waxflower at 0 to 1 °C (32 to 34 °F). If
properly precooled and subsequently wrapped in
The major postharvest problems are the shattering plastic to reduce water loss, flowers can be stored
of flowers and petals, an ethylene-related problem dry for up to 2 weeks at 1 °C (34 °F). There is no
that can be controlled by pretreating the flowers benefit of wet over dry storage, and dry storage is
with 1-MCP or STS, and Botrytis infection, therefore the method of choice. The main problem
particularly an issue for growing areas with during storage is from growth of Botrytis on
erratic rainfall. Fungicide dips have been used flowers. Dipping in 1% Rovral (Ipridione) solution
successfully as a control measure. prior to storage can prevent Botrytis infection.
Waxflowers are normally harvested with a Waxflowers are packed in bunches that contain
mixture of open flowers and mature buds on each variable numbers of stems (around 10) to provide
branchlet. Branches are harvested to maximize a satisfactory bunch.
stem length without compromising the following
year’s crop. Avoid buying if leaves or flowers
have fallen off or are turning yellow. Special Considerations
705
Grading and Bunching References
As with other filler flowers, bunches are made by Bieleski, R.L., J. Ripperda, J.P. Newman, and
size or weight. M.S. Reid. 1992. Carbohydrate changes and leaf
blackening in cut flower stems of Protea eximia. J.
Amer. Soc. Hort. Sci. 117:124-127.
Ethylene Sensitivity
Bovy, A.G., G.C. Angenent, H.J.M. Dons, and
Like other Asteraceae, solidaster flowers are not A.C. van Altvorst. 1999. Heterologous expression
sensitive to ethylene. of the Arabidopsis etr1-1 allele inhibits the
senescence of carnation flowers. Molec. Breed.
5:301-308.
Pretreatments
Burge, G.K., E.R. Morgan, I. Konczak, and
Like Solidago, solidaster flowers probably would J.F. Seelye. 1998. Postharvest characteristics of
benefit from a cytokinin pulse treatment to delay Limonium ‘Chorus Magenta’ inflorescences. NZ.
leaf yellowing (Philosoph et al. 1995). J. Crop Hort. Sci. 26:135-142.
If flowers are too immature when harvested, they Cevallos, J.C. 1998. Temperature and the
may not develop to their maximum beauty. Treat Postharvest Biology of Cut Flowers. M.S.
like most other members of the chrysanthemum Dissertation, University of California, Davis, CA.
family. Leaves should be stripped from the stem
as they rot underwater and will foul the vase Dai, J., and R.E. Paull. 1995. Source-sink
solution. Make sure that buckets, vases, and relationship and Protea postharvest leaf
solution are kept clean. blackening. J. Amer. Soc. Hort. Sci. 120:475-480.
706
Han, S.S. 1992. Role of sucrose in bud Macnish, A.J., D.C. Joyce, P.J. Hofman, et al.
development and vase-life of cut Liatris spicata 2000. 1-Methylcyclopropene treatment efficacy in
(L.) Willd. HortScience 27:1198-2000. preventing ethylene perception in banana fruit and
grevillea and waxflower flowers. Austral. J. Exp.
Heyes, J.A., and J.W. Johnston. 1998. Agric. 40:471-481.
1-Methylcyclopropene extends Cymbidium orchid
vase-life and prevents damaged pollinia from Mor, Y., A.H. Halevy, A.M. Kofranek, and M.S.
accelerating senescence. New Zealand J. Crop Reid. 1984. Postharvest handling of lily of the nile
Hort. Sci. 26:319-324. flowers. J. Amer. Soc. Hort. Sci. 109:494-497.
Hicklenton, P.R. 1991. GA3 and Naidu, S.N., and M.S. Reid. 1989. Postharvest
benzylaminopurine delay leaf yellowing in cut handling of tuberose (Polianthes tuberosa L.).
Alstroemeria stems. HortScience 26:1198-1199. Acta Hort. 261:313-317.
Ichimura, K., and K. Suto. 1999. Effects of the Newman, J.P., L.L. Dodge, and M.S. Reid.
time of sucrose treatment on vase-life, soluble 1998. Evaluation of ethylene inhibitors for
carbohydrate concentrations and ethylene postharvest treatment of Gypsophila paniculata L.
production in cut sweet pea flowers. Plant Growth HortTechnology 8:58-63.
Regul. 28:117-122.
Philosoph-Hadas, S., S. Meir, I. Rosenberger,
Jacob, Y., R. Barrade, M.J. Marquier, and E. and A.H. Halevy. 1995. Control and regulation of
Botton. 1997. Breeding of Anemone coronaria the gravitropic response of cut flowering stems
tetraploid hybrids. Acta Hort. 430:503-508. during storage and horizontal transport. Acta Hort.
405:343-350.
Jones, R.B., M. Serek, and M.S. Reid. 1993.
Pulsing with Triton X-100 improves hydration and Philosoph-Hadas, S., S. Meir, I. Rosenberger,
vase-life of cut sunflowers (Helianthus annuus and A.H. Halevy. 1996. Regulation of the
L.). HortScience 28:1178-1179. gravitropic response and ethylene biosynthesis
in gravistimulated snapdragon spikes by calcium
Joyce, D.C. 1993. Postharvest floral organ fall in chelators and ethylene inhibitors. Plant Physiol.
Geraldton waxflower (Chamelaucium uncinatum 110:301-310.
Schauer). Austral. J. Exp. Agric. 33:481-487.
Pritchard, M.K., C.S. Hew, and H. Wang. 1991.
Joyce, D.C., M.S. Reid, and R.Y. Evans. 1990. Low-temperature storage effects on sugar content,
Silver thiosulfate prevents ethylene-induced respiration and quality of anthurium flowers. J.
abscission in holly and mistletoe. HortScience Hort. Sci. 66:209-214.
25:90-92.
Rajitha, B.S., S.G. Rao, Y.B.N. Rao, and D. Singh.
Kappers, I.F., W. Jordi, F.M. Maas, et al. 1998. 1999. Post harvest effect of fluoride and other
Gibberellin and phytochrome control senescence chemicals on the vase-life of Gaillardia pulchella
in alstroemeria leaves independently. Physiologia cut flowers. J. Phyto. Res. 12:107-108.
Plantarum 103:91-98.
Reid, M.S., M. Mokhtari, J.H. Lieth, et al. 1996.
Kuiper, D., S. Ribot, H.S. van Reenen, and N. Modeling the postharvest life of cut roses. Acta
Marissen. 1995. The effect of sucrose on the Hort. 424:137-144.
flower bud opening of ‘Madelon’ cut roses. Sci.
Hort. 60:325-336. Serek, M., R.B. Jones, and M.S. Reid. 1995.
Physiology of flower senescence in gladiolus. Acta
Hort. 455-459:16-21.
707
Sexton, R., A.E. Porter, S. Littlejohns, and S.C. Wernett, H.C., T.J. Sheehan, G.J. Wilfret, et
Thain. 1995. Effects of diazocyclopentadiene al. 1996. Postharvest longevity of cut-flower
(DACP) and silver thiosulfate (STS) on ethylene Gerbera. I. Response to selection for vase-life
regulated abscission of sweet pea flowers components. J. Amer. Soc. Hort. Sci. 121:216-221.
(Lathyrus odoratus L.). Ann. Bot. 75:337-342.
708
Flower Bulbs Hertogh and Le Nard (1993a) and specific articles
cited. The second group is the “commercial
August A. De Hertogh and Marcel Le Nard bulbs,” which are primarily used for forcing
either as fresh-cut flowers or as potted growing
De Hertogh is formerly with the Department and flowering plants, indoor or outdoor container
of Horticultural Science, North Carolina State plants, outdoor fresh-cut flower production, and
University, Raleigh, NC. He is now retired. Le gardens and landscapes (De Hertogh and Le Nard
Nard is with Station d’Amélioration de la Pomme 1993c).
de Terre et des Plantes à Bulbes, Institut National
de la Recherche Agronomique, Ploudaniel, France.
Horticultural Objectives
Requirements
Taxon Storage Packing Storage
temperature
°C
Achimenes Prevent from drying Peat 10 to 15
Acidanthera Dry and ventilated None 20
Allium
giganteum Dry and ventilated None 25 to 28
Other alliums Dry and ventilated None 20 to 23
Alstroemeria Prevent from drying Peat 1 to 3
Amaryllis
belladonna Prevent from drying Peat 13 to 23
Anemone
blanda & Dry and ventilated None 9 to 17
fulgens
coronaria
(summer) Dry and ventilated None 15 to 25
(winter) Dry and ventilated None 10 to 13
Requirements
Taxon Storage Packing Storage
temperature
°C
711
Table 1. Packing and storage temperature requirements of ornamental flower bulbs—
continued
Requirements
Taxon Storage Packing Storage
temperature
°C
Requirements
Taxon Storage Packing Storage
temperature
°C
Lilium
longiflorum Prevent from drying Peat 2 to 7
Hybrids & Prevent from drying Peat and polyethylene 2 to -2
other species
Oxalis
adenophylla Prevent from drying Wood shavings 17 to 20
deppei Dry and ventilated None 2 to 5
Polianthes Dry and ventilated None 20
Puschkinia Dry and ventilated Wood shavings 20 to 23
Ranunculus
Summer Dry and ventilated None 17 to 20
Winter Dry and ventilated None 10 to 13
713
Table 1. Packing and storage temperature requirements of ornamental flower bulbs—
continued
Requirements
Taxon Storage Packing Storage
temperature
°C
714
About 75% of the flower bulbs used in the short periods, most bulbs will be physiologically
United States are imported, and the remainder injured (Van Aartrijk 1995 and 2000).
are domestically produced. In addition, all
production areas are distant from the sites of Table 2. Hardiness classifications for
use, and essentially 99% must be transported ornamental flower bulbs
for a few days to several weeks. Thus, all
trucks and shipping containers must be able to Hardiness Injured at
provide the proper temperature, ventilation, and classification temperatures below
moisture requirements for the bulbs (table 1). The
normal rate of air exchange for bulbs requiring Tender I 20 °C
ventilation during transport is 150 m3 h-1 (De Tender II 10 °C
Hertogh and Le Nard 1993c). In addition, for Tender III 2 °C
bulbs like hyacinths and dutch iris, the RH must Semi-hardy -2 °C
be controlled to minimize the development of Hardy I -5 °C
diseases such as Penicillium. Therefore, on arrival, Hardy II -10 °C
all bulb shipments must be carefully inspected Hardy III -15 °C
for serious diseases and insects, ventilated as
needed, and then properly stored (table 1). Also,
stage of development of the apical meristem must Ventilation
be determined. De Hertogh (1996) summarized
packing, transportation, and storage requirements When the taxa require ventilation (table 1), it
for forcing bulbs. is essential that the flower bulbs receive proper
air circulation and exchange during all periods
of postharvest storage. Thus, the application
Major Postharvest Storage Factors of these requirements must begin immediately
after the grower harvests the bulbs and continue
Temperature during transportation and subsequent handling
by the wholesaler, forcer, and retailer. The exact
Blaauw and his coworkers (Hartsema 1961) ventilation requirement is generally very specific.
clearly demonstrated that temperature was the It can be very low (Eremurus and Liatris, which
most important factor controlling the growth and must be prevented from drying out) or very high
development and flowering processes of flower (tulips, in which ventilation assists in preventing
bulbs. They demonstrated that precise control was flower abortion and abnormalities and in removing
required and that the specific temperature optima ethylene). In general, ventilation rates must be
varied for each bulb species and the desired time adapted to the storage temperature; that is, higher
of flowering. The optimal temperatures can range temperatures require higher rates of ventilation.
from -2 to 34 °C (28 to 93 °F) depending on the
bulb species, use, growing season, and cultivar.
Examples for the six major taxa are provided Moisture
below.
There are two basic considerations for the
Subsequently, De Hertogh and Le Nard (1993d) moisture level used for bulb storage (table 1). The
classified the hardiness of flower bulbs into first is to provide the optimum moisture level,
seven groups (table 2). This classification is very which will prevent desiccation of susceptible
important for the storage of nonplanted bulbs bulbs. Thus, materials such as moist peat or
(table 1) because they have limited protection wood shavings are used in combination with the
against extremes in temperatures. If they are various packaging materials such as polyethylene.
stored or transported at temperatures lower or The second is the control of RH during storage
higher than the required temperatures, even for or transportation, which can minimize root
715
development and prevent the development conditions controlling their development can
of diseases such as Penicillium on hyacinths, assist in either eliminating and/or minimizing their
Narcissus, tulips, and dutch iris. In addition, this effects during postharvest storage.
approach is an important technique to minimize
the use of fungicides either prior to or after
shipping. Since the registration of fungicides Major Flower Bulb Taxa
varies with each country and is always subject to
change, users must consult with their appropriate Gladiolus (Gladioli)
governmental agency to determine which
pesticides are approved. Cohat (1993) reviewed the physiology, pests, and
other aspects of Gladiolus. Many diseases can
affect gladioli in storage, and prestorage fungicidal
Ethylene dips are generally used. Current registrations must
be consulted for approved fungicides. Corms for
With the exception of dutch iris (see information commercial uses should be transported at 2 to 5 °C
below), nonplanted flower bulbs must not (36 to 41 °F) under highly ventilated conditions,
be exposed to ethylene. Most bulbs are about 2 m3 h-1 per 100 L of corms. With the
physiologically sensitive to ethylene levels of 0.1 exception of a hot water treatment (see below),
µL L-1 and higher. In general, when the flower has unplanted corms should never be stored at <0.5 °C
been formed and is immature, this plant growth or >30 °C (Cohat 1993, Van Aartrijk 1995).
regulator causes either floral abnormalities or
complete flower abortion. For very sensitive Planting stock. After being harvested, cleaned,
bulbs, such as tulips, it is advisable to have the and then graded, corms and cormlets used as
storage rooms analyzed for ethylene. Commercial planting stock are initially given 2 to 3 weeks
ethylene services are available to monitor the level at 15 to 23 °C (59 to 73 °F) followed by 2 °C
of ethylene in all floriculture facilities. (24 °F) under highly ventilated conditions for a
minimum of 8 to 10 weeks. The latter is required
to break the rest period (dormancy) of the corms
Modified Atmospheres and cormlets. Prior to planting, they are given 4
to 8 weeks at 20 to 30 °C (68 to 86 °F) to promote
Research on the modification of the O2, N2, and sprouting. To control Fusarium, cormlets are
CO2 levels of the storage atmosphere has been stored at 20 to 25 °C (68 to 77 °F) for 8 to 12
reported for gladioli, lilies, and tulips (De Hertogh weeks, then soaked in running water for 1 to 2
and Le Nard 1993d). In Europe, this system is days at 20 °C (68 °F), and then given a hot water
referred to as “ultra-low oxygen” (ULO) and is treatment of 55 to 56 °C (131 to 133 °F) for 30
used commercially in the Netherlands for lily and min.
tulip bulb storage (Anonymous 1996). However,
the specific procedures used and the results have Commercial bulbs for outdoor use. Horticul-
not been published in refereed journals. turally, the major uses for gladioli corms are
outside either for use as commercial fresh cut
flowers or in gardens and landscapes. The large
Diseases and Insects flowering corms for these uses must be provided
with the same storage temperatures and conditions
All users of flower bulbs must be familiar with as the planting stock. Corms used for very long
the diseases and insects that affect each species storage and thus for very late flowering must be
(Baker 1993, Byther and Chastagner 1993, Van stored at 2 °C (36 °F) until planted.
Aartrijk 1995, 2000). There are many pests, but
usually there are only a few major ones for each
species. Knowledge of the major pests and the
716
Hyacinthus (Hyacinths) Commercial bulbs for gardens and landscaping.
After being harvested, bulbs for these uses are
Nowak and Rudnicki (1993) reviewed the stored dry and under highly ventilated conditions
physiology, pests, and other aspects of hyacinths. at 25.5 °C (78 °F). They are shipped and
Commercially, the Netherlands produces almost subsequently stored at 17 °C (63 °F).
all of the bulbs used world wide. The major
storage disease for commercial sized hyacinth
bulbs is Penicillium. It can be controlled with Iris hollandica (Dutch Iris)
optimum ventilation conditions and a RH of 85
to 90%. Hyacinth bulbs should be transported De Munk and Schipper (1993) reviewed the
and subsequently stored at 17 °C under highly physiology, pests, and other aspects of dutch iris.
ventilated conditions. With the exception of the The two major storage diseases of dutch irises are
heat treatment to control Xanthomonas hyacinthii Fusarium and Penicillium. To assist in controlling
(see below), unplanted bulbs are never stored at these diseases, it is essential to always handle the
<0 °C or >25.5 °C (78 °F) (Nowak and Rudnicki bulbs carefully to minimize mechanical damage,
1993, Van Aartrijk 2000). which provides sites for disease development.
Prestorage and preplanting fungicidal dips are
Planting stock. The postharvest handling and generally used for dutch iris. Current registrations
storage of small hyacinth bulbs require precise must be consulted for approved fungicides.
conditions to control the bacterium Xanthomonas After harvest and up to planting, dutch iris bulbs
hyacinthii (yellow disease). After harvest, the should be stored at 50 to 60% RH to minimize
bulbs are stored at 30 °C under highly ventilated root growth and development of Penicillium. In
and dry conditions until September 1; they are general, unplanted bulbs should never be stored
then given 2 weeks at 38 °C, followed by 3 days at <0 °C (32 °F) or >30 °C (86 °F) (De Munk and
at 44 °C, and subsequently 25.5 °C (78 °F) until Schipper 1993, Van Aartrijk 1995).
planted. Bulbs used for scooping and scoring and
other large planting stock bulbs are stored under Planting stock. The primary objective during
dry and highly ventilated conditions at 25.5 °C (78 postharvest storage of the bulbs is to prevent
°F) from harvest until planted. flower formation in order to produce round,
commercial-sized bulbs in the field. In addition,
Commercial bulbs for forcing. Two types of the conditions should prevent the development of
commercial bulbs are available for greenhouse diseases. The control of flower initiation is very
forcing as either potted plants or fresh cut flowers. critical in 7/8-cm circumference planting stock
The first is “prepared bulbs,” which are used for bulbs. This size group is known as “the in-between
very early forcing. After being harvested in early size,” since larger bulbs flower readily and smaller
June, the bulbs are stored under dry and highly ones do not. These bulbs should be provided with
ventilated conditions for 2 weeks at 30 °C (86 °F), the following postharvest temperatures: 23 °C
followed by 3 weeks at 25.5 °C (78 °F), and then (73 °F) from harvest to 1 September, followed by
23 °C (73 °F) until the uppermost floret reaches 2 weeks at 30 to 35 °C (86 to 95 °F), and then,
stage A2. Subsequently, they are stored at 17 °C depending on the cultivar and bulb size, 5 to 9 °C
(63 °F) until planted. “Regular bulbs,” which are (41 to 48 °F). Bulbs that are smaller than 7/8 cm
used for medium and late forcing, are given 25.5 in circumference should be stored at 18 to 20 °C
°C (78 °F) followed by 4 weeks at 17 °C (63 °F) (64 to 68 °F).
prior to planting. De Hertogh (1996) provided
detailed forcing programs for hyacinths in North Commercial bulbs for forcing. Generally, bulbs
America. for very early forcing are stored at 30 °C for a
few days after being harvested. This is necessary
for bulb drying, cleaning, and grading. They are
717
subsequently exposed to 500 µL L-1 ethylene for sized bulbs” are immediately packed in moist peat
24 h. This treatment stimulates flower initiation, in wooden cases and then sent to either the forcer
especially with small bulbs of some cultivars and or commercial bulb jobber. To flower properly,
bulbs not receiving sufficient heat in the field the bulbs must receive 6 weeks at 2 to 7 °C (36
before being harvested. Bulbs for late and retarded to 45 °F) either by case precooling in moist peat
(up to 1 year) forcing are placed at 30 °C (86 °F) or by being controlled-temperature forced in pots
to suppress their growth and development. Prior in a moist planting medium. De Hertogh (1996)
to planting, they are “precooled” at 5 to 9 °C (41 provided forcing schedules for Easter lily in North
to 48 °F) for 6 to 11 weeks, depending on the America.
cultivar. After being precooled, bulbs should be
planted as quickly as possible and, if transported, Hybrid lilies. Beattie and White (1993) reviewed
the period should be as short as possible. De the physiology, pests, and other aspects of the
Hertogh (1996) provided forcing programs for hybrid and species lilies. Breeding of these lilies
dutch iris used as fresh cut flowers in North is expanding rapidly and many new cultivars
America. are continuously being released. After being
harvested, the bulbs are graded and cleaned. To
Commercial bulbs for outdoor uses. Dutch irises properly flower them, the bulbs must be packed in
are grown outdoors as commercial fresh cut moist peat, wrapped in polyethylene, and packed
flowers and in gardens and landscapes. After being in trays for storage and shipping. Subsequently,
harvested, these bulbs are initially stored at 30 °C they require at least 6 to 8 weeks at 2 °C (36 °F)
(86 °F) and then shipped and stored at 17 °C (63 before being planted for early forcing. For late
°F) until planted. When necessary, bulbs can be and year-round forcing the bulbs are “frozen-in”
precooled for 3 to 4 weeks at 5 to 9 °C (41 to 48 at -1 to -2 °C (28 to 30 °F) after being precooled
°F). for 6 to 8 weeks. Modified atmospheres (ULO)
systems, although not new (Stuart et al. 1970),
are being used for long storage of some hybrids
Lilium (Lilies) (Anonymous 1996). This system is being used
because there are fewer negative effects of the
In North America two basic types of commercial long-term period of being frozen-in (Van Aartrijk
lilies are generally used: the Easter lily (L. 2000). An alternative to this system is to produce
longiflorum), which is mainly used as a flowering the bulbs in the Southern Hemisphere for fall
potted plant, and a wide range of hybrid and planting in the Northern Hemisphere (Anonymous
species lilies, which are used either as fresh-cut 2001). Planting stock is stored at 2 °C (36 °F)
flowers, as flowering potted plants, or in gardens in moist peat until planted. De Hertogh (1996)
and landscapes. When properly packed in moist provided forcing guidelines for Asiatic and
peat, unplanted lily bulbs can be stored at -1 to -2 Oriental lilies in North America.
°C (28 to 30 °F), but are normally never stored
above 17 °C (63 °F) (Beattie and White 1993,
Miller 1993, Van Aartrijk 2000). Narcissus (Hardy Daffodils and Paperwhites)
Easter lily. Miller (1993) reviewed the physiology There are two basic groups of Narcissus, the
of forcing and bulb production, pests, and “Hardy Narcissus” (daffodils) and the nonhardy
other aspects of the Easter lily. These bulbs “Paperwhite Narcissus.” Hanks (1993) reviewed
are produced in the coastal areas of Northern the physiology, pests, and other aspects of these
California and Southern Oregon. Immediately groups. With the exception of the hot water
after being harvested and graded, the “planting treatment (see below), nonplanted hardy Narcissus
stock” is planted in the field. The “commercial- bulbs are never stored at >34 °C (93 °F) (see early
718
forcing below), while paperwhites are not stored Tulipa (Tulips)
at >30 °C (86 °F) (Hanks 1993). Neither group
of bulbs is stored at <0 °C (32 °F) (Van Aartrijk Le Nard and De Hertogh (1993b) reviewed the
1995). physiology, pests, and other aspects of tulips.
Without question, tulips are the largest taxa of
Hardy Narcissus. By far, the largest group of flower bulbs grown and used throughout the
Narcissus comprisesthe hardy cultivars, and world. Hundreds of cultivars are produced for
hundreds are grown in many countries. The major forcing as fresh cut flowers, as potted growing
storage diseases are Fusarium and Penicillium plants, and in gardens and landscapes. The major
(Byther and Chastagner 1993, Van Aartrijk storage diseases are Fusarium and Penicillium.
1995). To control Fusarium in the planting Normally, Fusarium infections begin in the field
stock, approved fungicides are added during a and continue during postharvest storage. There are
hot water treatment (HWT) of 1 to 2 h at 44 °C substantial differences in cultivar susceptibility,
(112 °F). This treatment also controls nematodes but it is important to reduce the number of
and bulb flies. Penicillium can be controlled by infected bulbs in storage because of the ethylene
use of proper ventilation conditions and a RH of they produce. This can lead to flower abortion and
85 to 90%. Except for the HWT, planting stock abnormalities. Thus, the bulbs must be routinely
bulbs are stored at 17 to 20 °C (63 to 68 °F). inspected and the rooms monitored for ethylene;
Commercial bulbs for early forcing are given levels in the storage rooms must not exceed 0.1
1 week at 34 °C (93 °F) followed by 17 °C (63 µL L-1. In storage, Penicillium can be controlled
°F). All of the other bulbs are provided 17 to 20 with high rates of ventilation and RH of 85 to
°C (63 to 68 °F). De Hertogh (1996) provided 90%. Regardless of the horticultural use, tulip
forcing programs for hardy Narcissus cultivars in bulbs should be shipped at 17 °C (63 °F) under a
North America. Transportation of hardy daffodils ventilation rate of 150 m3 h-1. With the exception
either for forcing or garden and landscape use of the 34 °C (93 °F) treatment for early forcing
should be at 17 °C (63 °F) under highly ventilated (see below), nonplanted bulbs are never stored at
conditions. >25 °C (77 °F) or <0 °C (32 °F) (Le Nard and De
Hertogh 1993b, Van Aartrijk 2000).
Paperwhite Narcissus. Most cultivars of
paperwhite Narcissus bulbs are produced in Israel. Planting stock. After being harvested, bulbs are
They are not considered to be hardy bulbs but can initially placed at 23 to 25 °C (73 to 77 °F) for
be planted outdoors in USDA Climatic Zones 9 to 3 to 4 weeks. Subsequently, depending on the
11 (Cathey 1990). Planting stock and commercial cultivar, they are stored at progressively lower
bulbs are stored at 25 to 30 °C (77 to 86 °F) after temperatures, from 23 to 20 °C (73 to 68 °F) down
being harvested. Commercial bulbs should be to 17 to 15 °C (63 to 59 °F), until just before being
shipped at 25 to 30 °C (77 to 86 °F) under highly planted. These temperatures are used to encourage
ventilated conditions. After arrival, bulbs should enhanced production of large sized bulbs from the
be stored at 25 to 30 °C (77 to 86 °F) until shoots planting stock.
begin to emerge. Subsequently, they should be
placed at 2 °C (36 °F). Prior to planting, they Forcing bulbs. For very early forcing, most
require 2 weeks at 9 to 17 °C (48 to 63 °F). De cultivars are given 34 °C (93 °F) for 1 week
Hertogh (1996) provided forcing programs for immediately after lifting. Subsequently, they are
Israeli-grown paperwhites in North America. stored under dry and well-ventilated conditions at
17 to 20 °C (63 to 68 °F). It is very important to
be able to identify the stages of flower initiation
(Hartsema 1961), since most cultivars must reach
stage G prior to being precooled at 5 to 9 °C (41
to 48 °F). For medium and late forcing, bulbs
are placed at 17 to 23 °C (63 to 73 °F) prior to
719
planting at low temperatures. De Hertogh (1996) Cathey, M. 1990. USDA Plant Hardiness Zone
provided forcing programs for cut and potted Map. Miscellaneous Publication 1475, U.S.
tulips in North America. However, bulbs are also Department of Agriculture, Washington, DC.
grown in the Southern Hemisphere for planting in
the fall in the Northern Hemisphere (Anonymous Cohat, J. 1993. Gladiolus. In A.A. De Hertogh,
2001). As with the hybrid lilies, this eliminates the and M. Le Nard, eds. The physiology of flower
need for and effects of long storage periods. bulbs. pp. 297-320. Elsevier Science Publishers,
Amsterdam, Netherlands.
Garden and landscaping bulbs. The key to use
of tulip bulbs in North America is the USDA De Hertogh, A.A. 1996. Holland Bulb Forcer’s
Climatic Zone (Cathey 1990). In northern Guide, 5th ed. Intl. Flower Bulb Centre, Hillegom,
climatic zones, they naturally receive adequate Netherlands.
cold exposure to satisfy their low temperature
requirement. In warm climatic zones, they must be De Hertogh, A.A., and M. Le Nard. 1993a. The
precooled at 5 °C (41 °F) for 8 to 10 weeks prior Physiology of Flower Bulbs. Elsevier Science
to planting. Normally after being harvested, tulip Publishers, Amsterdam, Netherlands.
bulbs for gardens and landscape use are stored at
20 to 23 °C (68 to 73 °F) and then shipped and De Hertogh, A.A., and M. Le Nard. 1993b.
stored at 17 °C (63 °F) prior to being planted. Botanical aspects of flower bulbs. In A. De
Hertogh and M. Le Nard, eds., The Physiology
of Flower Bulbs, pp. 7-20. Elsevier Science
References Publishers, Amsterdam, Netherlands.
Anonymous. 1996. Resultaten ULO-bewaring De Hertogh, A.A., and M. Le Nard. 1993c. World
lelies hoopvol. CNB INFO 551:19. production and horticultural utilization of flower
bulbs. In A. De Hertogh and M. Le Nard, eds., The
Anonymous. 2000. Inventarisatie Physiology of Flower Bulbs, pp. 21-28. Elsevier
wereldbollenareaal. Bloembollencultuur 21:4. Science Publishers, Amsterdam, Netherlands.
720
Hanks, G.R. 1993. Narcissus. In A. De Hertogh Van Aartrijk, J. 1995. Ziekten en Afwijkingen bij
and M. Le Nard, eds., The Physiology of Flower Bolgewassen. Deel 2: Amaryllidaceae, Araceae,
Bulbs, pp. 463-558. Elsevier Science Publishers, Begoniaceae, Cannaceae, Compositae Iridaceae,
Amsterdam, Netherlands. Oxalidaceae, Ranunculaceae. Tweede Druk.
Laboratorium voor Bloembollenonderzoek, Lisse,
Hartsema, A.M. 1961. Influence of temperatures Netherlands.
on flower formation and flowering of bulbous and
tuberous plants. In W. Ruhland, ed., Handbuch Van Aartrijk, J. 2000. Ziekten en Afwijkingen
der Pflanzenphysiologie, pp. 123-167. Springer- bij Bolgewassen. Deel 1: Liliaceae. Derde Druk.
Verlag, Berlin. Laboratorium voor Bloembollenonderzoek, Lisse,
Netherlands.
Le Nard, M., and A.A. De Hertogh. 1993a. Bulb
growth and development. In A. De Hertogh and
M. Le Nard, eds., The Physiology of Flower
Bulbs, pp. 29-43. Elsevier Science Publishers,
Amsterdam, Netherlands.
721
Flowering Potted Plants 1 to 2 weeks after shipping. Research over the last
25 years has concentrated on factors providing for
Terril A. Nell retention of leaf color while maximizing flower
longevity.
Nell is with the Environmental Horticulture
Department, University of Florida, Gainesville, Cultivar selection and production conditions
FL. affect the response of flowering potted plants
to shipping conditions. Chrysanthemum and
poinsettia cultivars vary considerably in their
Transporting and storing flowering potted plants ability to withstand shipping. Hibiscus cultivars
challenges commercial growers’ continuing ability drop buds and flowers as a result of improper
to provide a high quality product. Quality suffers shipping conditions. It is likely that differences
when plants are exposed to adverse shipping and in cultivar response of other flowering potted
storage conditions, such as exclusion from light plants to shipping conditions exist, but extensive
in closed containers and sleeves, exposure to research has not been conducted to elucidate these
harmful gases and temperature extremes, poor responses.
air ventilation, high RH, and vibration. These
conditions can lead to deterioration of even the Production conditions can play a major role
highest quality plants. Further, the environmental in the ability of potted flowering plants to
and physical stresses imposed on plants during withstand shipping conditions. High fertilizer
transit are worsened if plants are improperly levels during production decreases the quality
produced, incorrectly packaged, or mishandled of chrysanthemums, campanula, poinsettia, and
during shipping or upon receipt. other plants during and following shipping. In
chrysanthemum, terminating fertilizer at flower
Flowering potted plants represent a significant color (3 weeks prior to marketing) resulted in
portion of floriculture production in the United a 7- to 11-day increase in longevity, depending
States (20.3% of production), with 53% of the on cultivar and fertilizer rate. With potted roses,
production in six States: California, Florida, overwatering of the plants during the final 1 to 2
Texas, Pennsylvania, North Carolina, and New weeks of production results in rapid losses in plant
York. Plants are often produced at locations distant and flower quality following shipping as a result
from the point of marketing; Thus shipping, often of damage to the root system.
for long distances, has become commonplace in
the industry. The extended shipping or storage Four factors—disease, improper temperature,
times may result in loss of quality and reduced extended shipping duration, and exposure to
longevity. ethylene—result in either rapid loss of quality
during shipping or reduced longevity and quality
Flowering potted plants range from cold tolerant following shipping. All of these factors can be
to chilling sensitive and from ethylene insensitive interrelated in their effects. For instance, packing
to ethylene sensitive. Quality and longevity are flowering potted plants in a warm greenhouse or
based on flower longevity and leaf quality. A packing area then placing the box into a cooler
flowering potted plant with yellow leaves has will result in condensation on the flowers and
little value even if the flowers last for a long leaves, providing ideal conditions for botrytis,
period. Likewise, plant quality is diminished powdery mildew, or other diseases. Similarly, use
when the flower dies rapidly though the leaves of optimum temperatures may cause problems
remain green. Problems with shipping may not be during long shipping times.
apparent immediately following shipping: Buds or
flowers may drop several days after unboxing, or Production and shipping practices should
leaves may turn yellow or flowers die prematurely minimize the potential incidence of diseases. For
instance, calcium sprays and reduced fertilizer
722
have been shown to minimize the incidence of Table 1. Recommended shipping
poinsettia bract edge burn in the greenhouse, temperatures for flowering potted plants
during shipping, and in the retail setting. In most
cases, diseases, especially botrytis and powdery Shipping temperature
mildew, will become worse during shipping 35 to 40 °F 50 to 60 °F
because of the high RH microclimate created in (2 to 5 °C) (10 to 15 °C)
the closed shipping box.
Amaryllis Africian violet
Temperature management is one of the best
Azalea Begonia-elatior
methods of maintaining quality during shipping.
Calceolaria Bougainvillea
Reduced temperatures lead to lower respiration,
Chrysanthemum Browallia
conservation of carbohydrate reserves, and
Cineraria Christmas cactus
minimize problems associated with ethylene.
Crocus Clereodendron
Optimum shipping temperature varies with
species, but plants should be shipped at the lowest Cyclamen Crossandra
possible temperature (table 1). Chilling-sensitive Daffodil Cymbidium
crops are generally shipped at 50 to 53 °F (10 to Easter lily Easter cactus
12 °C), while those that are not chilling sensitive Freesia Exacum
are shipped at 35 °F (2 °C) to maximize plant and Grape hyacinth Gloxinia
flower quality. Hyacinth Hibiscus
Hydrangea Poinsettia
Ethylene can adversely affect quality. Plants Kalanchoe Streptocarpus
may produce ethylene, or plants may be exposed Oxalis
to ethylene from external sources, such as Regal geranium
combustion engines and dead and decaying Rose
organic matter (fruit, vegetables, or flowers). Tulip
Ethylene is a colorless, odorless gas that can cause
many undesirable effects on flowering potted Adapted from Sterling and Molemaar (1985) and
plants at very low levels—25 to 100 nL L-1 (ppb). Nell (1993).
Typical ethylene injury symptoms include leaf and
bud drop, premature aging, and leaf yellowing, but
other disorders have also been identified (table 2).
723
Table 2. Response of flowering potted Several chemicals are available commercially that
plants to ethylene1 will minimize the detrimental effects of ethylene.
Application procedures and effectiveness on
Crop Symptoms ethylene-sensitive crops vary, but these chemicals
can be a valuable tool for crops that exhibit
Achimenes Flower/bud drop ethylene injury. The use of anti-ethylene chemicals
African violet Flower wilting is especially valuable on chilling-sensitive crops,
Azalea Leaf drop since temperature cannot be reduced.
Begonia-elatior Flower drop
Bougainvillea Flower/bract drop Flowering potted plant quality can be maintained
Browallia Flower/bud drop during shipping provided that production practices
Carnation Failure of flower to open and cultivar are selected properly and optimum
Calceolaria Flower/bud drop shipping conditions are maintained, including
Clereodendron Flower/bract drop; leaf drop temperature management and prevention of
injury from ethylene. However, regardless of the
Crossandra Flower drop
conditions, shipping and storing flowering potted
Cyclamen Flower drop; flower wilting
plants for extended periods will lead to decreased
Cymbidium Wilting of the sepal
longevity. Ideally, flowering potted plants should
Exacum Flower wilting
be stored and shipped for brief periods at optimum
Geranium Floret drop
conditions.
Gardenia Flower/bud drop
Gloxinia Flower drop
Hibiscus Flower/bud drop References
Kalanchoe Failure of flowers to open;
petal drying Nell, T.A. 1993. Flowering Potted Plants:
Pachystachus Petal wilting; bud blasting; Prolonging Shelf Performance. Ball Publishing
leaf yellowing Co., Batavia, IL
Poinsettia Petiole droop2
Streptocarpus Flower drop Sterling, E.P., and W.H. Molemaar. 1985.
The influence of time and temperature during
Adapted from Woltering (1987). simulated shipment on the quality of potplants.
Rapport Sprenger Instituut no. 2286, Springer
1
The degree of sensitivity to ethylene varies with Institute, Wageningen, The Netherlands
plant species, variety, ethylene concentration,
and temperature during exposure and duration of Woltering, E.J. 1987. Effects of ethylene on
exposure. ornamental pot plants: classification. Scientia
2
Petiole droop (epinasty) of poinsettia is caused Hort. 31:283-294
by upward bending of leaf and bract petioles
during sleeving.
724
Foliage Plants In simulated shipping tests at 10, 13, 16, and
19 °C (50, 55, 61, and 66 °F) for 1 to 4 weeks
Terril A. Nell in the dark, Scheffiera arboricola shipped best
at 19 °C, Ficus benjamina at 10 or 13 °C, and
Nell is with the Environmental Horticulture Dracaena marginata equally well at 13, 16, and
Department, University of Florida, Gainesville, 19 °C. These plants withstood shipment for 3
FL. weeks without a significant loss of quality and for
4 weeks, at some temperatures, without severe
quality reduction. Shefflera plants recovered
Foliage plant production and sales represent a from dark storage within 17 days after transfer
significant part of ornamental plant production to light. Ficus plants are damaged by holding at
in the United States. In 1998, sales were $509 4 °C (39 °F) for 6 or more days. Similar chilling
million, with the greatest production in Florida injury can occur on many foliage plaints at
(61%), California (18%), and Texas (4%). temperatures of 7 °C (45 °F) for long periods or
Consequently, foliage plants are shipped long 2 to 5 °C (36 to 41 °F) for even short periods.
distances to supply retail markets throughout Although 1 day at 5 °C (41 °F) may not damage
North America and Europe. most foliage plants, severe damage can occur
when plants are subjected to cool temperatures
Temperature control is critical to successful over a 3- to 5-day transit time. Coleus is very cold
shipping and quality retention in the darkness of sensitive. Dracaena sanderana and Spathiphyllum
transport vehicles. The shipping environment is clevelandii are severely injured after 1 day at 2
not conducive to maintaining foliage plant quality. °C (36 °F) and slightly after 1 day at 10 °C (50
Although it is difficult to generalize, the best °F). Aglaonema is very intolerant of temperatures
shipping temperature is in the range of 15 to 18 °C <10 °C (<50 °F). Exposure to 4.4 °C (40 °F) for 2
(59 to 64 °F) with 85 to 90% RH. Temperatures days causes severe damage to Scindapsus pictus
of 10 to 13 °C (50 to 55 °F) are the lowest that (satin pothos) and Maranta leuconeura (prayer
should be considered for shipping, and even at this plant). Fittonia verschaffeitii (silvernerve plant) is
range some chilling injury may occur with some severely damaged within 8 h at 2 °C (36 °F).
plants.
Foliage plants should be turned over rapidly in
Foliage plants should be acclimatized by growers marketing and should not be overordered. Plants
prior to shipment. Acclimatization is the process should not be stored in back rooms for extended
of making plants more tolerant to environmental periods or in severe drafts of heat or cold. Foliage
changes during or after shipment. Growers plants should not be transported with fruits,
acclimatize plants using lower fertilizer levels and vegetables, or cut flowers, most of which emit
temperatures or reduced light and water during the ethylene and are shipped at low temperatures.
last 2 to 4 weeks before shipment. Acclimatized Plants such as scheffleras, crassulas, fittonias, and
plants are better adapted to dark storage in transit ficuses are very sensitive to ethylene.
and reduced light in stores than nonacclimatized
plants. Requirements and length of shipping
tolerated vary because of the wide diversity
of foliage plants. Shipping temperatures have
been identified for some acclimatized plants in
temperature-controlled containers (table 1). These
guidelines are based on simulated shipping tests in
darkness.
725
Table 1. Suggested shipping temperatures for acclimatized foliage plants to
maintain quality in refrigerated vans1
°C °F °C °F
Aglaonema, cv. ‘Fransher’ 13-16 55-60 16-18 60-65
Aglaonema, cv. ‘Silver Queen’ 16-18 60-65 16-18 60-65
Ardisia crispa 10-13 50-55 — —
Aspidistra elatior 10-13 50-55 — —
Brassaia actinophylla 10-13 50-55 10-13 50-55
Chamaedorea elegans 13-16 55-60 — —
Chamaedorea seifrizii 13-16 55-60 — —
Chrysalidocarpus lutescens 13-18 60-65 16-18 60-65
Codiaeum variegatum 16-18 60-65 16-18 60-65
Cordyline terminalis 16-18 60-65 — —
Dieffenbachia picta 16-18 60-65 — —
Dracaena deremensis 16-18 60-65 — —
Dracaena fragrans 16-18 60-65 — —
Dracaena marginata 13-18 55-65 16-18 60-65
Ficus benjamina 13-16 55-60 13-16 55-60
Ficus nitida 13-16 55-60 — —
Howeia forsteriana 10-18 50-65 10-18 50-65
Nephrolepis exaltata 16-18 60-65 — —
Peperomia bicolor 16-18 60-65 — —
Philodendron selloum 13-16 55-60 — —
Philodendron oxycardium 16-18 60-65 — —
Phoenix roebelenii 10-13 50-55 — —
Pleomele reflexa 16-18 60-65 — —
Rhapis excelsa 10-13 50-55 — —
Schefflera arboricola 10-13 50-55 10-13 50-55
Scindapsus aureus 16-18 60-65 — —
Spathiphyllum, Mauna Loa 10-13 50-55 13-16 55-60
Yucca elephantipes 10-13 50-55 10-13 50-55
1
Data are for plants in containers in the dark. Some plants stored without lights for 10 to 14 days
will show slight to severe leaf loss and/or yellowing but will recover.
2
Blanks indicate that plant’s tolerance to shipping beyond 15 days is unknown.
726
Herbaceous Perennials from storage and shipped to wholesale and retail
nurseries.
Arthur C. Cameron
Grower experience and research results have
established that many hardy herbaceous perennials
Cameron is with the Department of Horticulture,
will retain excellent regrowth quality after
Michigan State University, East Lansing, MI.
several months of storage when properly packed
to control moisture loss and stored at -2 °C (28
°F) (Walters 1983). Mahlstede and Fletcher
Introduction
(1960) published an excellent and comprehensive
summary of the requirements for storage of
Herbaceous (nonwoody) perennials are a
nursery stock (unfortunately out of print).
diverse group of plants grown primarily for
their ornamental value in gardens throughout
the world. Storage of herbaceous perennial plant
Field Conditions and Harvesting of Bare-
material may be required at several steps during
Root Crowns
the production and marketing of the final product,
which is often a containerized flowering plant
Rain and excess moisture in the field can cause
for retail sales. Some herbaceous perennials such
serious problems during storage, particularly
as strawberry, rhubarb, potato, and asparagus
if any free water from the harvested plants is
are grown as food crops, but the storage of their
not removed before storage. Crowns and roots
underground organs is discussed separately in
can be difficult to dry and can quickly develop
this handbook. Many herbaceous perennials have
surface molds and rots. Many growers prefer to
succulent stems and leaves that, in cold or dry
grow plants on sandy soils so that harvesting is
climates, die back in fall to an overwintering
interrupted as little as possible during wet periods
crown or root structure, while others cease growth
(Walters 1983).
but do not go dormant in response to inclement
weather.
Field-grown herbaceous perennials should be
harvested after the crowns are fully mature for
Herbaceous perennials include species in just
optimum storage success. Peony, iris, mertensia,
about every plant family and vary greatly in
and poppy can be harvested even before the first
adaptability, growth patterns, and tolerance to
freeze, since they tend to mature early in the fall.
heat and cold. In fact, it is the great diversity
However, many other hardy herbaceous perennials
that presents the most problems, including that
enter dormancy later in the season or not at all
of postharvest storage. Some nurseries carry
and should be harvested as late in fall as possible
hundreds if not thousands of species, and methods
for best storage success (Mahlstede and Fletcher
of production vary greatly.
1960, Hanchek and Cameron 1995). For most
hardy herbaceous perennials, sugars and nutrients
accumulate in the roots, crowns, and other
Storage of Bare-Root Crowns
underground organs, while leaves and above-
ground tissues senesce in response to short days,
Seedlings, rooted cuttings, or divisions can be
low temperatures, or both in a process similar to
field-planted, grown, and harvested as bare-root
that in potato, sugar beet, and certain other edible
(crowns and roots with soil removed) plants in
herbaceous perennials.
fall. Storage of the bare-root plants is convenient,
since they can be trimmed, processed, and
If harvested before complete maturation,
packaged during winter when harvest may not be
bare-root plants will have a much shorter
practical because of snow, ice, or rain. Throughout
storage life because of various factors such
winter and spring, bare-root plants are removed
as incomplete carbohydrate accumulation,
727
insufficient suberization of the roots and crowns, growth, it is not uncommon for plants to recycle
lack of overwintering buds, and ultimately the fibrous roots, meaning that most small roots die
development of molds and rots (Hanchek and during winter and are replaced the following
Cameron 1995). In practice, however, growers spring as growth commences. The dead and dying
often harvest prematurely in fall, especially in roots do not usually create problems outdoors but
northern regions, since snow can quickly end the can feed numerous surface molds while in storage.
harvest season. Growers must accept the extra The molds are unsightly and can lead to loss of
risk of reduced storage life imposed by the earlier product (Hanchek et al. 1990). Fungicides are
harvest. seldom used to control molds, in part because of
the great diversity of plant types and sensitivity.
When possible, plants should be harvested after
tops have at least begun to die back, often after Some herbaceous perennials such as Dicentra
the first hard freeze. Some species never really spectabilis (bleeding hearts) have more
go dormant and do not completely die back to the specialized crowns and roots. These enlarged
ground except in the coldest of winters. In some roots hold substantial food reserves and can be
instances, the tops can be cut with a rotary mower successfully stored for several months when
to remove excess leaves and stems before harvest harvested mature. However, when harvested
to reduce postharvest cleaning and grading and immature, bleeding hearts are very susceptible
potentially increase storage success. However, to surface molds (Hanchek et al. 1990). In many
most species that do not attain full dormancy are respects, this process is similar to maturation and
difficult to store for long periods, since many have storability of potato tubers.
wounded green tops with a high respiration rate,
fibrous roots, and insufficient carbohydrate storage
reserves. Also, the more green tissue removed, the Postharvest Grading and Packaging
more likely that the crowns are not fully mature.
Ideally, harvested roots and crowns should be
processed, packed, and immediately cooled
Classification of Perennials by Root for long-term storage. In practice, herbaceous
Characteristics perennials are often harvested in fall faster
than they can be processed. Crowns, roots,
Mahlstede and Fletcher (1960) suggested and remaining above-ground tissue are rapidly
several categories of perennials based primarily respiring immediately after harvest because of
on structural or physical characteristics of wounding, and to prevent fermentation it is critical
the overwintering underground structures. to keep plants cool and to provide adequate
Considering the diversity of herbaceous ventilation for the tissue. Initial plant temperature
perennials, the overwintering root structure can depends on soil and air temperatures during
provide one additional piece of information that harvest. Harvested plants should be quickly cooled
can be used to develop storage recommendations. to 0 °C (32 °F) to reduce respiration and moisture
loss, though precooling seldom occurs in practice.
Greentops are one group of herbaceous perennials Temperatures of harvested material should be
that overwinter with at least some above- monitored closely with hand-held or automated
ground leaf and stem tissue remaining. Green thermometers. There is a real possibility of
tissue increases the storage difficulty for a great fermentation when O2 movement is restricted
percentage of the plants in this category. Difficult- in large piles or tightly packed crates of plants,
to-store greentops include ajuga, arabis, alyssum, particularly those with green tops or fibrous root
creeping phlox, coreopsis, dianthus, and gaillardia. systems. If temperatures rise in stacks of plant
material, then it is critical to add refrigeration,
Numerous difficult-to-store herbaceous perennials improve ventilation, or both.
have fibrous roots. During the natural cycle of
728
Excess green tissue is usually removed during crates, or bins should be placed into cold storage
grading, and plants may be cut into smaller in rows that maximize air movement and cooling
divisions for propagation. The wounds caused rate. They can be restacked closer together after
during division can be a source of subsequent they reach storage temperature.
infection if not properly healed before storage.
Even typically easy-to-store plants such as
hosta and daylily can be lost during long-term Optimum Storage Conditions
storage because of fungal and bacterial wound
infection. Conditions favoring wound healing The optimum storage temperature for most
could be useful for these crops after grading. No hardy herbaceous perennials is between -2.8 and
studies have been published on wound healing -2.2 °C (27 to 28 °F) (Mahlstede and Fletcher
of harvested herbaceous perennials, but wound 1960, Maqbool and Cameron 1994). At these
healing and suberization of potato tubers occurs temperatures, tissues are generally not frozen,
most rapidly at 15 to18 °C (60 to 65 °F) and <90% and respiration and water activity are minimized.
RH. However, not all herbaceous perennials can
withstand subfreezing temperatures, and the
Packaging depends on the intended means of following are best stored at 0 to 2 °C (32 to 34
storage. Unsealed polyethylene liners (1.5 to 3 °F) (Mahlstede and Fletcher 1960, Maqbool and
mils thick) are used for -2 °C (28 °F) storage. Cameron 1994): aconitum, agapanthus, althaea,
At below-freezing storage temperatures, these anchusa, aentranthus, helleborus, hibiscus, malva,
liners permit O2 movement adequate to supply shasta daisy.
the needs of respiration and protect against water
loss (Cameron 1988). These same liners can lead Some perennials have very poor regrowth
to fermentation and loss of product at higher following storage, regardless of pretreatments and
temperatures, since they may limit air movement storage conditions, and are best overwintered as
to such an extent that the supply of O2 can not potted plants. These plants, such as gaillardia and
meet the increased demands of respiration. Many coreopsis, can be stored as small containerized
herbaceous perennials are packaged in multiples plants packed into polyethylene-lined boxes up
of 25 with the liners in a cardboard box ready to several months. Hibiscus, alcea, sidalcea, and
for shipping later in the season. It is important to other malvaceous plants are sensitive to low
match box size to crown size since overpacking temperature storage, even in minimally heated
can also lead to fermentation and loss of product, greenhouses. Short-term storage of these genera
particularly for plants with green tops, fibrous should be at warmer temperatures. Herbaceous
roots, or both. Wood fibers or other dry fillers perennials considered very difficult to store for
(such as shredded paper) should be added inside extended durations (Mahlstede and Fletcher 1960)
the polyethylene liner to absorb free moisture are alcea rosea, anemone, anthemis, asclepias,
(condensation etc.) and improve gas exchange clematis, coreopsis, epimedium, gaillardia,
inside the package. helleborus, hibiscus, lavender, lithospermum,
malva, primula, sidalcea.
In some cases, bare-root plants are stored in bulk
bins (often the same as bins used for storage of
apples). Although appropriate for some otherwise Problems During Storage of Bare-Root
difficult-to-store plants, bulk bins can lead to Perennials
problems if extra care is not taken to ensure
that the entire contents are cooled quickly. Some herbaceous perennials are difficult to
Temperatures at the center of the bin need to be store as bare-root plants for extended periods.
monitored closely, especially for the first 2 to 3 Even closely related plants can have different
weeks. Heating, fermentation, and eventually overwintering capacities. Much depends on the
decay will start at the middle of the piles. Boxes, species, overall plant vigor, and size in the field
729
before harvest. Problems encountered during Storage of Herbaceous Perennials in
storage of bare-root plants include development Plug Trays
of rots and surface molds, desiccation, and growth
of fragile buds after the dormancy requirement There has been a complete transition in
has been met. Each of these problems increases herbaceous perennial production over the past
with storage duration and temperature. Hourly two decades. Most herbaceous perennials are now
or daily temperature fluctuations can increase propagated, from either seeds or cuttings, in plug
tissue desiccation, condensation on the package, trays with anywhere from 32 to 516 small plugs
and ultimately surface molds and rots, so it is (one plug is an individual cell with one plant) per
important to maintain constant temperature into 30×60 cm (12×24 in) tray by using techniques
spring. borrowed largely from the bedding plant industry.
The shift to plug production has been hastened
Molds and bacteria can establish on dead roots or by improved uniformity of seed-propagated
wounded tissue early during storage, especially if herbaceous perennials and increased demand
plants are not cooled rapidly. Some surface molds for large numbers of plants. Typically, plugs
do not seriously harm the regrowth capacity of the are finished within 6 to 12 weeks of seeding or
crowns (Maqbool and Cameron 1994). However, placement of the cutting. Producers often sell their
they are unsightly and often cause rejection of a product to finishers—greenhouse growers who
shipment, which can represent a substantial loss pot plugs into larger containers and grow them for
of revenue, considering the time and effort spent retail sale. Traditionally, plugs are purchased and
producing and storing the plants up to the time containerized in fall, and finished plants are sold
they are sold. Molds are invariably more severe at the following spring or summer.
elevated temperatures or when there is significant
condensation, particularly if packages are poorly When started from seeds or cuttings, many young
ventilated or stacked without sufficient air herbaceous perennial plants require a period of
circulation. Free water must be absorbed or it will cold, or vernalization, before they can achieve
increase mold and rot development. Limited trials optimum regrowth potential (Whitman et al. 1996,
with fungicides for control of surface molds have Runkle et al. 1998). A cold period of 10 to 15
been inconclusive (Hanchek et al. 1990). weeks may be needed to fulfill the vernalization
requirement for some herbaceous perennials
Bud-break can occur on many perennials such as (Whitman et al. 1996), though 6 to 8 weeks is
asters, shasta daisies, geums, lupines, bleeding more common (Cameron et al. 2000). The cold
hearts, and astilbes kept at 1 °C (34 °F) after their requirement is delivered to containerized plants
dormancy requirement has been satisfied. New in overwintering greenhouses with supplemental
growth is usually etiolated and extremely fragile heating to prevent severe cold damage. Bedding
(Maqbool and Cameron 1994). Bud-break can plants are sometimes stored as plugs, though
occur during shipping, since lack of temperature most traditional bedding plants do not have a cold
control is common. Astilbes and bleeding heart, requirement. It is becoming increasingly common
often shipped from the Netherlands, commonly to store plugs at low temperatures to economize
have new shoot growth in the packages, on the amount of controlled temperature space
presumably because of long shipping times at required. Plugs can be stored for convenience (as
elevated temperatures. Storage temperatures when the finish date precedes the marketing date)
below 0 °C (32 °F) completely prevented bud- or to provide a physiological requirement for
break for all herbaceous perennial species tested subsequent flowering. Cooled plugs can be sold at
(Maqbool and Cameron 1994). In some instances, a premium price when there is no need for further
refrigerated storage facilities do not have adequate cooling.
cooling capacity, so temperatures can rise in
spring as the cooling requirement increases. Many species of perennials can be stored as plugs
Premature bud-break can be a serious problem in for more than 3 mo at 5 °C (41 °F), but only when
these storage facilities.
730
provided with sufficiently intense light to perform Mahlstede, J.P., and W.E. Fletcher. 1960. Storage
photosynthesis. Photosynthesis supplements of Nursery Stock. Amer. Assn. Nurserymen,
carbohydrate supplies, which can be depleted Washington, DC.
during storage, particularly in small plugs. Engle
(1994) studied storage of plugs at -2.5 °C (28 Maqbool, M., and A.C. Cameron. 1994. Regrowth
°F) in a manner analogous to that described performance of field-grown herbaceous perennials
for bare-root plants. Plug trays were packed in following bare-root storage between -10 and +5
polyethylene liners and stored in the dark without °C. HortScience 29:1039–1041.
any additional water. Many perennial species
grown as plugs did not survive direct transfer Runkle, E.S., R.D. Heins, A.C. Cameron, and
from the greenhouse to subfreezing temperatures. W.H. Carlson. 1998. Flowering of Leucanthemum
However, 3 to 6 weeks of hardening at 0 or 5 °C × superbum ‘Snowcap’ in response to photoperiod
(28 or 41°F) improved storage success for many. and cold treatment. HortScience 33:1003–1006.
As for bare-root storage, plugs with significant
green tissue were more difficult to store. Walters, J. 1983. Overwintering in the field
and in storage. In E.M. Smith, and S.M. Still,
Several growers are experimenting with plug eds., Proceedings of the Herbaceous Perennial
storage under controlled conditions, though most Symposium, July 1983, Columbus, OH. Ohio
storage still takes place in minimally heated Cooperative Extension Service Bull. no. 717, Ohio
greenhouses. If growers rent storage facilities, State University, Columbus, OH.
they should ensure that refrigeration capacity is
adequate to reduce storage temperatures to at least Whitman, C.M., R.D. Heins, A.C. Cameron, and
2 °C (35 °F). Insufficient cooling will increase W.H. Carlson. 1996. Cold treatment, photoperiod,
respiration and plant demand for photosynthetic and forcing temperature influence flowering of
light. Lighting the plugs will improve success. Lavandula angustifolia. HortScience 31(7):1150–
1153.
References
731
Orchids their sensitivity to ethylene. Research also showed
that pulsing excised Dendrobium flowers with 0.5
Yin-Tung Wang mM AOA for 24 h before exposing them to 0.2 µL
L-1 ethylene did not prolong their life (Wang and
Wang is with the Agricultural Experiment Station, Wang 2000); all treated flowers wilted within 3
Department of Horticultural Sciences, Texas A&M days after exposure. Thus, AOA has no effect on
University, Weslaco, TX. longevity when wilting is triggered by exogenous
ethylene.
Introduction
Treatments for Extending Shelf-Life
For years, potted orchids were produced
mainly for hobbyists. They are usually shipped It was found that shelf-life of Oncidium Goldiana
in containers and arrive in buyers’ hands by cut sprays increased after pulsing with silver
overnight delivery or within a few days. Many nitrate for 30 min (Ong and Lim 1983). Pulsing
orchid growers do not ship orchids during winter Arranda cut inflorescences for a short 10 min
months to avoid chilling or freezing injury to the with a 4 mM solution of STS prolonged their
plants. However, during the past decade, there display-life (Hew et al. 1987). Exposing excised
has been a tremendous worldwide boom in orchid Dendrobium and Phalaenopsis flowers to 0.1 µL
production for sale as potted blooming plants L-1 1-methylcyclopropene (1-MCP, an ethylene
for the public, particularly the Phalaenopsis and action ihibitor) for 12 h, or pulsing them with 0.5
Dendrobium orchids. Potted, blooming orchids mM silver thiosulfate (STS, an ethylene inhibitor)
represent a $100 million industry in the United for 24 h completely blocked the deleterious effect
States alone (NASS 2001). However, for the most of ethylene (Wang and Wang 2000). Phalaenopsis
part, research in proper postharvest handling flowers that were treated with 0.1 to 0.4 µL L-1
of orchids has lagged behind the increase in of 1-MCP are protected against ethylene as high
production and demand. as 10 µL L-1. However, the protective effect of
1-MCP lasts for no longer than 7 days at room
temperature. Commercially, cut Phalaenopsis
Ethylene Sensitivity inflorescences are pulsed with 0.5 mM STS
immediately following cutting.
Orchid flowers are extremely sensitive to ethylene,
even at very low levels (Davison 1949, Beyer When used at 50 to 100 mg L-1,
1976, Goh et al. 1985). Pollination and removal 8-hydroxiquinoline sulfate (8-HQS) extended
of pollen caps (emasculation) trigger ethylene shelf-life of Dendrobium Pompadour cut flowers
production by flowers and result in rapid wilting. (Kesta and Amutiratana 1986). Conflicting results
Blooming orchids should be handled carefully so were reported on the effect of sucrose in keeping
that accidental pollination and emasculation do solutions as a carbon source for extending vase
not occur. Accelerated flower wilting as a result life of cut orchid flowers.
of ethylene evolution following pollination in
Cymbidium, Dortitaenopsis, Dendrobium, and Sprays of tropical orchids should be stored and
Phalaenopsis orchids is induced by a loss of water transported at 12 to 18 °C (54 to 64 °F) (Akamine
from cells of the upper layer of the petals, leading 1976), whereas flowers of temperate orchids such
to their in-folding and water-soaked appearance as Cymbidium may be stored at temperatures as
(Lee and Lin 1992, Porat et. al. 1994). Treating low as 5 °C (41 °F) (Sheehan 1954).
orchid flowers with the ethylene biosynthesis
inhibitor aminooxyacetic acid (AOA) did not alter
732
Optimum Storage Conditions Shipping Information
Minimal research has been conducted on optimum Because of quarantine regulations, internationally
orchid storage conditions. In one study (Wang shipped orchids are often removed from their
1997a), bare-root Phalaenopsis plants were potting medium. The bare-root plants are allowed
packed in boxes and subjected to temperatures of to dehydrate slightly before packing. This is
15, 20, 25, or 30 °C (59, 68, 77, or 86 °F) for 4, done for several reasons. Slight dehydration
7, or 14 days. Weight loss from plants increased allows for packing more plants per box, avoids
with increasing temperature and storage duration. breaking leaves, and reduces the chances of
Symptoms of chilling injury were inversely related moisture buildup to weaken shipping boxes. Dryer
to 15 and 20 °C storage temperatures. Chilling plants also reduce incidence of rotting before
injury became more severe as storage duration boxes are opened. Plants are usually packed in
increased. Plants had little or no chilling injury at layers alternating with shredded paper or other
25 or 30 °C, regardless of the duration. Leaf loss material. Often, plants are individually wrapped in
was most severe on plants stored at 15 °C for 7 newspaper, particularly those that have developed
or 14 days or 30 °C for 14 days. Plants that have inflorescences.
severe leaf loss after storing at 30 °C have delayed
spiking and flowering. Millions of bare-root Phalaenopsis orchids are
shipped domestically and internationally each
Preconditioning greenhouse-grown potted year. After the orchids are planted in pots and
Phalaenopsis plants at 25 °C (77 °F ) for 10 placed on greenhouse benches, yellow spots
days, followed by another 10 days at 20 °C (68 occasionally develop on either side of the mid-
°F), reduced severity of chilling injury after rib near the base of leaves due to chilling injury.
storage at 15 °C (59 °F) for 2 weeks. Therefore, Irregular yellow blotches also occur on other parts
Phalaenopsis orchids harvested during the warm of leaf blades. Other times, leaves turn yellow
period of the year should be held above 20 °C (68 and fall off, resulting in weak plants that do not
°F), and preferably closer to 25 °C (77 °F), during produce flowers.
shipping. Under such conditions, plants can lose
as much as 20% of their fresh weight at harvest
without subsequent performance being adversely References
affected. While most Phalaenopsis species and
hybrids need relatively cool air to initiate the Akamine, E.K. 1976. Postharvest handling
flowering process, plants do not respond to cool of tropical ornamental cut crops in Hawaii.
air while being shipped in the absence of light HortScience 11:125-126.
(Wang 1995).
Beyer, E.M. 1976. A potent inhibitor of ethylene
Although prolonged duration in complete darkness action in plants. Plant Physiol. 58:268-271.
is not recommended for keeping plants in bloom
(Wang and Blessington 1989), Phalaenopsis Davison, O.W. 1949. Effect of ethylene on orchid
orchids tolerate low light levels once they have flowers. Proc. Amer Soc Hort Sci. 53:440-446.
started flowering. One study show that exposing
them to irradiance levels between 10 and 50 Hew, C.S., K.H. Wee, and F.Y. Lee. 1987. Factors
μmol m-2 sec-1 for 12 h daily produced orchids affecting the longevity of cut Aranda flowers. Acta
with similar longevity (individual flowers lasting Hort. 205:195-202.
over 120 days) and flower size (Wang 1997b).
Therefore, most retail stores have adequate light Goh, C.J., A.H. Halevy, R. Engel, and A.M.
levels to maintain blooming Phalaenopsis while Kofranek. 1985. Ethylene evolution and
they are being marketed. sensitivity in cut orchid flowers. Sci. Hort. 26:57-
67.
733
Kesta, S., and D. Amutiratana. 1986. Effect of Wang, M.K., and Y.T. Wang. 2000. 1-MCP and
sucrose, silver nitrate, and 8-hudroxyquinoline STS counteract ethylene effects on cut orchid
sulfate on postharvest behavior of Dendrobium flowers. HortScience 35(3):349.
Pompadour flowers. In Proceedings of the 6th
ASEAN Orchid Congress, Bangkok, Thailand, pp.
124-129. Association of Southeast Asian Nations,
Bangkok, Thailand.
734
Seeds and Pollen Recalcitrant seeds are produced by herbaceous
plants from aquatic habitats (for example,
Chris Walters and Leigh Towill wildrice, watercress, and wasabi), perennials
from tropical areas (examples include avocado,
Walters and Towill are with Preservation of Plant mango, coconut, cocoa, and jackfruit), and some
Germplasm Research, National Center for Genetic deciduous forest trees from temperate areas (such
Resources Preservation, Agricultural Research as oak, chestnut, buckeye, and silver and sycamore
Service, U.S. Department of Agriculture, Fort maple). As the name implies, recalcitrant seeds are
Collins, CO. difficult to store and have shelf-lives usually <1
year unless they are cryopreserved.
735
Table 1. Guidelines to identify storage behavior of seeds
Growth habit Most herbaceous plants produce orthodox seeds Aquatic species
Habitat Many aquatic species, tropical rainforest species, Most native Hawaiian species,
and temperate climax forest species produce temperate conifers, some
recalcitrant seeds maples
Water content Most orthodox seeds dry naturally on the All immature seeds,
at harvest parent plant Solanaceae, Cucurbitae
Seed size Recalcitrant seeds are often large Some aquatic species,
Rutaceae, some Rubiaceae
Desiccation Orthodox seeds can survive complete water loss; Orthodox seeds dried very
sensitivity recalcitrant seeds cannot slowly (for >2 weeks) can be
severely damaged
Seed Storage Behavior and General maximum longevity. Intermediate seeds survive 1
Storage Principles to 6 mo if stored at 25 °C (77 °F) and 2 to 5 years
if stored at 5 °C (41 °F). These seeds rapidly lose
Because they are sensitive to drying, recalcitrant viability if frozen (stored at -18 °C [0 °F]).
seeds must be stored at 92 to 98% RH. The best
storage temperature depends on the chilling Seeds that exhibit orthodox behavior are easily
sensitivity of the species. Seeds from many stored by drying and cooling. The extent to which
tropical fruits (mango, avocado, cocoa, jackfruit) drying and cooling extends shelf-life is best
are sensitive to chilling and should be stored at described by Harrington’s “Thumb rules” which
≥15 °C (59 °F). These seeds have the shortest state that the life of a seed is doubled for every 1%
potential shelf-life, remaining viable for 2 weeks decrease in water content or every 5 °C (41 °F)
to 3 mo. Seeds produced from temperate species decrease in temperature (Harrington 1963, Justice
such as oaks, buckeye, or chestnut can survive and Bass 1978). The “100s Rule” states that
for 0.5 to possibly 2 years by storing them at 2 adequate longevity for commercial seed storage
to 5 °C (36 to 41 °F). Microbial contamination purposes (<5 yr) can be achieved by ensuring that
is always a problem at high RH, and seeds often the sum of the RH and temperature in °F during
survive longer if a fungicide is applied. Storage storage does not exceed 100.
in damp peatmoss is often beneficial. The RH
required for safe storage of recalcitrant seeds often These rules were developed as guidelines for
allows seeds to germinate. Once this has occurred, commercial seed storage. They are not valid for
procedures for storage of seedlings should be extremely dry conditions, and their applicability
adopted. at extremely cold temperatures has not been
adequately tested.
Seeds with intermediate storage behavior are
more amenable to drying and consequently can A relatively new rule is that seeds store optimally
be preserved for longer periods than recalcitrant at 15 to 25% RH. Storing seeds with RH <15%
seeds. RH of 40 to 60% appears to provide will not increase shelf-life and may actually
736
accelerate deterioration (Walters 1998b). Seed of development on the parent plant. The high
water content achieved at 15 to 25% RH varies temperatures, >40 °C (>104 °F), frequently
from about 2 to 10%, depending on seed lipid used to rapidly dry immature seeds can severely
composition and storage temperature. damage them. Some drying procedures call for
exposure to 35 °C (95 °F) for several days. This
Storage at -18 °C (0 °F) (the temperature of a treatment can initiate seed aging processes. An
standard freezer) enhances shelf-life 4- to 5-fold initial treatment that dries immature seeds slowly
over that of seeds stored at 5 °C (41 °F) (Walters enhances seed desiccation tolerance (Vertucci
1998a). Storage temperature should be dictated by and Farrant 1995, Pammenter and Berjak 1999)
the required longevity for the seed: Storage at 15 and potentially seed longevity. If seeds must be
°C (59 °F) may be adequate for most species to harvested prematurely, holding them for 2 to 5
maintain quality for 3 to 5 years; longevities of 5 days in their fruiting structures can simulate slow
to 10 years may require refrigeration at 5 °C (41 drying. Once immature seeds receive a slight and
°F); while storage at -18°C (0 °F) is required if slow desiccation treatment, they can be threshed
seeds are to be maintained for 15 to 20 years. with less damage.
Numerous factors during seed development and Seed priming is a procedure used to accelerate
maturation affect the potential longevity of seeds. germination rates of planted seeds. Seeds are
Seeds harvested prematurely may have shorter usually held at <10 °C (<50 °F) with water
shelf-life than seeds harvested fully mature potentials close to -1 MPa for a few days. While
(defined as having completed maturation drying). this treatment gives the appearance of improving
Shelf-life may be shorter because immature seed vigor—seeds germinate faster or more
embryos are not fully tolerant of desiccation and uniformly—it exposes mature seeds to high RH
are damaged when dried, or because maximum and likely reduces their shelf-life if they are
seed quality is acquired during the final stages redried and then stored (Walters 1998a).
737
Anecdotal evidence suggests that seeds harvested
from the wild have a shorter shelf-life than
seeds harvested from cultivated plants. There
are numerous possible explanations. Through
agricultural practices, humans may have selected
for seeds with superior quality. Cultivated plants
may have received optimum growth conditions—
irrigation, fertility, and pest management—and
are therefore more robust. Seeds collected from
the wild are often highly immature because
maturation of uncultivated seeds is less uniform
and mature fruits often dehisce.
738
Table 2. Approximate longevity for various seed storage behaviors and seed species
% g H2O per g dw
Recalcitrant Tea (Camellia sinensis) 95 to 98 0.6 to 0.8 2 weeks to 2 months
Buckeye (Aesculus
hippocastanum) 95 to 98 0.5 to 0.7 6 to 8 months
Trifoliate orange
(Poncirus trifoliata) 90 to 95 0.5 to 0.7 6 to 14 months
Wildrice (Zizania
palustris) 90 to 95 0.35 to 0.45 9 to 18 months
739
1
For orthodox seeds, the lower value in the water content range is more appropriate for 5 °C
(41 °F) storage, and the higher value approximates optimum water contents for constant
storage at -18 °C (0 °F). Water content on a percentage dry weight or fresh weight basis are
often used in the seed industry. To calculate percentage water content on a dry weight basis,
multiply water contents in the table by 100.
2
Values for approximate longevity are given for 5 °C (41 °F) storage for all species except
Camellia sinensis, which is chilling sensitive and should be stored at 15 °C (59 °F).
While species have characteristic longevities, should not be stored in a lighted room without a
variability among cultivars and among lots of the protective container.
same cultivar may be so great that it precludes
prediction of shelf-life. For example, germination
of sesame seeds with initial germination rates Procedures to Dry Orthodox Seeds
of 70 to 80% ranged from 0 to 80% after 18
years storage at the National Center for Genetic At storage temperatures >0 °C (32 °F), seed water
Resources Preservation. Similar results were content or RH are the most important factors
obtained for potato, pepper, sorghum, onion, and determining seed longevity. Seed producers must
tomato: initial rates of germination from 90 to consider two factors when drying seeds: the rate
100% and rates after 18 years of 0 to 100%. The at which seeds are dried and the level to which
source of this variability is unknown. Suppliers they are dried. With the exception of immature
should monitor seed viability periodically to seeds that benefit from a short, slow, drying
ensure quality. period, orthodox seeds with high water content,
>18%, should be dried to about 12% as rapidly
as possible. Temperatures >35 °C (95 °F) can be
Additional Storage Factors Affecting damaging, so lower temperatures and high air flow
Shelf-Life are recommended. Seeds should be spread out in
a thin layer to allow air to circulate through the
In addition to RH and temperature, the gas seed mass and gently mixed daily to ensure even
composition of the storage environment may be drying.
important, but evidence for a clear relationship
is lacking. Low O2 should enhance longevity In temperate or cold climates, no special storage
since aging reactions are either oxidative or conditions are required if seeds are to be planted
linked to respiration. Storage under higher O2 the next season, that is, within 6 mo. Storage of
tensions causes rapid deterioration (Ohlrogge dried seeds in open bins with no RH control is
and Kernan 1982), but the converse has not been called “open storage,” and the water content of
demonstrated. Freeze-drying or vacuum packaging the seed will fluctuate according to the RH and
may be beneficial (Woodstock et al. 1976, Justice temperature in the warehouse. Open storage is not
and Bass 1978, Ellis et al. 1993), but it is not recommended in warm humid regions or if seeds
clear whether these treatments control moisture will be planted several years after harvest.
level or O2 level. Reducing the RH is a far more
effective method of limiting respiratory processes The water content of seeds must be controlled
than lowering O2. The time and expense of using if seeds are to retain high quality for >2 years.
low O2 is probably not worthwhile for short-term This is easily accomplished by placing seeds
storage of orthodox seeds. at a known RH once they have been dried to
a safe water content of <12%. There are two
Exposure to light, even at low intensities, appears strategies: Dried seeds can be packed in moisture-
to damage dry seeds. Seeds intended for nursery permeable bags and stored in rooms where the
stock should not be dried under the sun and RH is controlled, or they can be equilibrated at
740
the desired RH and then packaged in moisture- When seed producers opt to store seeds
impermeable containers (for example, glass, in moisture-proof bags, they increase the
foil laminate, or aluminum cans). The RH in the complexity of water content adjustments but
moisture-proof containers is a function of the also the flexibility in choosing drying and
water content of the seeds and the temperature at storage conditions. The interested reader may
which they are stored. refer to more in-depth literature on water
sorption isotherms of seeds (Walters 1998a,b).
A RH of 20 to 25% produces optimal seed water The guiding principle is that 20 to 25% RH
content for storage of most orthodox species provides optimum storage. However, since
(Walters 1998a,b). The equilibrium water content RH is a function of temperature, both the
varies among seeds according to lipid composition drying and storage temperature need to be
and storage temperature. For example, peanut considered. Generally, seeds are dried at ambient
seeds with 45% lipid will contain 2 to 5% water, temperatures or slightly higher to speed up the
while pea seeds with 2% lipid will contain 8 to drying process and to reduce refrigeration costs
12% water. When RH during storage is controlled, (Walters-Vertucci and Roos 1996). The greater
water content of seeds need not be monitored. the temperature differences between drying and
The simplicity of this method may cause some storage temperatures, the higher the allowable
producers to use it exclusively; however, constant RH for drying. For instance, if seeds are dried at
dehumidification, especially in conjunction with 25 °C (77 °F) and stored at 15, 5, or -18 °C (59,
refrigeration, may be prohibitively expensive 41, or 0 °F), they should be equilibrated to about
(Walters-Vertucci and Roos 1996). Depending on 30, 38, or 45% RH, respectively. Table 3 gives a
volume of seeds handled and risk of mechanical brief summary of recommended drying conditions
failure, it may be safer and more cost-effective to for given storage temperatures. To date, these
store seeds in moisture-proof containers. guidelines have proven reliable for all species
tested.
°C %
25 30 38 45
15 22 30 38
5 15† 22 30
*The given drying temperature and RH combinations give storage RH of 20-25% at the
indicated storage temperature.
†
Drying seeds at temperatures less than the storage temperature is not cost effective and
therefore is strongly discouraged; dehumidification is more difficult at lower temperatures,
and the refrigeration costs used during drying might be more effectively spent during storage.
741
Cost-Benefit Analyses aging occurs can be monitored with initial and
subsequent germination assays. Different seed
Drying and storage conditions used by seed species have different germination requirements
producers and suppliers largely depend on a that are cataloged in Rule Books published by
balance between the cost of seed preservation and the Association of Official Seed Analysts (1999).
the necessity to maintain high quality seeds for Assessments of seed vigor are more difficult than
a certain period. The three main questions that assessments of germination percentage but usually
should be considered when deciding appropriate provide an early warning of deterioration.
storage conditions are—
References
Assessing Changes in Seed Quality
Association of Official Seed Analysts. 1999. Rules
No storage procedure guarantees that seeds will for Testing Seeds. Association of Official Seed
remain viable forever. Seeds eventually lose vigor Analysts, Lincoln, NE.
and then viability with time. The extent to which
742
Barnabas, B., and G. Kovacs. 1997. Storage of Pammenter, N.W., and P. Berjak. 1999. A review
pollen. In K.R. Shivanna and V.K. Sawhney, eds., of recalcitrant seed physiology in relation to
Pollen Biotechnology for Crop Production and desiccation-tolerance mechanisms. Seed Sci. Res.
Improvement, pp. 293-314. Cambridge University 9:13-37.
Press,Cambridge, U.K.
Priestley, D.A. 1986. Seed Aging: Implications for
Ellis, R.H, T.D. Hong, M.C. Martin, et al. 1993. Seed Storage and Persistance in the Soil. Cornell
The long term storage of seeds of seventeen University Press. Ithaca, NY.
crucifers at very low moisture contents. Plant Var.
Seeds 6: 75-81. Schopmeyer, C.S. 1974. Seeds of Woody Plants in
the United States. Agriculture Handbook 450, U.S.
Hanna W.W., and L.E. Towill. 1995. Long-term Department of Agriculture, Washington, DC.
pollen storage. In J. Janick, ed., Plant Breeding
Reviews, vol. 13, pp. 179-207. Wiley & Sons, Vertucci, C.W., and J.M. Farrant. 1995.
New York, NY. Acquisition and loss of desiccation tolerance. In J.
Kigel and G. Galili, eds., Seed Development and
Harrington, J.F. 1963. Practical advice and Germination, pp. 237-271. Marcel Dekker, New
instructions on seed storage. Proc. Intl. Seed Test. York, NY.
Assoc. 28:989-994.
Walters, C. 1998a. Understanding the mechanisms
Hoekstra, F.A. 1995. Collecting pollen for genetic and kinetics of seed aging. Seed Sci. Res. 8:223-
resources conservation. In L. Guarino, V.R. 244.
Rao, and R. Reid, eds., Collecting Plant Genetic
Diversity: Technical Guidelines, pp. 527-550. Walters, C., ed., Recent Advances on Ultra-dry
CAB Intl., Oxfordshire, U.K. Research. In Seed Science Research, vol. 8,
Supplement 1, International Society for Seed
Hong, T.D., and R.H. Ellis. 1996. A protocol to Science, Cambridge UniversityPress, Cambridge,
determine seed storage behaviour. IPGRI Tech. U.K.
Bull. 1, International Plant Genetic Resources
Institute, Rome, Italy. Walters-Vertucci, C., and E.E. Roos. 1996. Seed
moisture, seed drying and energy costs of a seed
Hong, T.D., S. Linnington, and R.H. Ellis. bank. In Report of the 50th Annual Corn and
1996. Seed Storage Behaviour: A Compendium. Sorgham Research Conference, pp. 243-255.
Handbooks for Genebanks no. 4, International American Seed Trade Association, Alexandria,
Plant Genetic Resources Institute, Rome, Italy. VA.
Justice, O.L., and and L.N. Bass. 1978. Principles Woodstock, L.W., J. Simkin, and E. Schroeder.
and Practices of Seed Storage. Agriculture 1976. Freeze drying to improve seed storability.
Handbook 506, U.S. Department of Agriculture, Seed Sci. Technol. 4: 301-311.
Washington, DC.
743
Almond Quality Characteristics and Criteria
The low water and high fat contents of the kernel The low water content of properly stored kernels
make it relatively stable metabolically and able to makes them relatively inert metabolically.
tolerate low temperatures. The primary objectives Respiratory rates are very low.
of storage regimes are to maintain the low water
content. Federal regulations define a safe moisture
level for nuts as a water activity <0.7 at 25 °C (77 Physiological Disorders
°F) to retard microbial growth. The recommended
storage RH is 65% because too low a water Two important quality problems of almond
content negatively affects flavor, color, and texture kernels are influenced by failure to maintain low
(Kader 1996). Cold storage is useful to minimize water content. Harvested nuts are often stockpiled
lipid oxidation (rancidification). In-shell almonds and fumigated to control naval orangeworm prior
can be stored for up to 20 mo at 0 °C (32 °F), 16 to hulling and shelling. Temperatures in covered
mo at 10 °C (50 °F), and 8 mo at 20 °C (68 °F). stockpiles that are open to the sun can reach 60
Shelled nuts can be stored for about half as long °C (140 °F). If nuts have not been dried to <10%
as nuts in the shell (about 6 mo), and pieces for moisture in the orchard, or have been wetted by
745
late-season rains, they should not be fumigated. kernels with insect damage will reduce the number
The combination of elevated moisture and of whole kernels with excess levels (>1 ng/g) of
temperature leads to a problem called concealed aflatoxins. However, lower grades and broken
damage, which is marked by inversion of sucrose, kernels or kernels sold as animal feed or processed
lipid oxidation, and internal kernel darkening. for oil can still present problems (Schatzki 1996).
Elevated temperature alone does not cause the Sampling of kernels to test for aflatoxin presents
problem. Wetting of freshly harvested almonds statistical problems because contamination is
followed by heating can cause the problem, and centered in individual kernels.
forced-air drying of rain-wetted kernels can
prevent it. However, almonds that have been held
in dry storage for several months and then wetted Quarantine Issues
and heated do not develop the problem (Reil et al.
1996). The most serious almond postharvest insect
problem is with naval orangeworm. The insect
Another kernel quality problem is premature lays its eggs in newly split nuts just before harvest,
sprouting (growth of the embryo between the and the resulting larvae can cause substantial
unopened cotyledons). losses. Fumigation with methyl bromide, which
has a limited future, or phosphine is used to
While the almond kernel’s content of control the insect. In-home control of insects can
polyunsaturated fatty acids is much less use treatment at freezing temperatures of -5 to
than walnuts or pecans, failure to maintain -10 °C (14 to 23 °F) for a few days. Irradiation
proper storage lead to rancidification. Factors can also be used (30 kilorad). The most useful
contributing to rancidification were studied by nonchemical insect control approach appears
Zacheo et al. (1998). to be a <1% O2 and 9 to 9.5% CO2 CA regime
(Guadagni et al. 1978).
Postharvest Pathology
References
Most infections with pathogens are initiated in the
orchard, and because clean-up following harvest is Abdallah, A., M.H. Ahumada, and T.M. Gradziel.
not absolute, the potential problems are transferred 1998. Oil content and fatty acid composition of
to the postharvest environment. In-shell product almond kernels from different genotypes and
is relatively protected unless the shell has been California production regions. J. Am. Soc. Hort.
broken or penetrated by insects. The most serious Sci. 123:1029-1033.
pathogens are fungi such as Aspergillus flavus
and A. parasiticus, which can produce aflatoxins Cavaletto, C.G., A.A. Kader, and D.E. Kester.
that are both toxic and carcinogenic. Damaged 1985. Quality in relation to marketability of
kernels must be discarded prior to storage and almond varieties. Report to the California Almond
low temperature and RH conditions must be Board. Pomology Department, University of
maintained. Phillips et al. (1979) and King et California, Davis, CA.
al. (1983) have described the variety of fungi
(primarily Aspergillus spp., but also Alternaria, Connell, J.H., G.S. Sibbett, J.M. Labavitch, and
Rhizopus, Cladosporium, and Penicillium spp.) M.W. Freeman. 1996. Preparing for harvest. In
that are found on almonds, and the latter work and W.C. Micke, ed., Almond Production Manual, pp.
King and Schade (1986) have described the effect 254-259. Pub. no. 3364, Division of Agriculture
of various water activities and temperatures on the and Natural Resources, University of California,
competition between these fungi on stored kernels. Davis, CA.
Because of the relationship between insect damage
and pathogen presence, sorting to eliminate whole
746
Guadagni, D.G., E.L. Soderstrom, and L. Storey. Zacheo, G., A.R. Cappello, L.M. Perrone, and
1978. Effect of controlled atmosphere on flavor G.V. Gnoni. 1998. Analysis of factors influencing
stability of almonds. J. Food Sci. 43:1077-1080. lipid oxidation of almond seeds during accelerated
ageing. Lebensm.-Wiss. Technol. 31:6-9.
Kader, A.A. 1996. In-plant storage. In W.C.
Micke, ed., Almond Production Manual, pp.
274-277. Pub. no. 3364, Division of Agriculture
and Natural Resources, University of California,
Davis, CA.
747
Hazelnut tolerance or resistance to allow production east of
the Rocky Mountains with some success.
Niels Maness
748
of the husk (Thompson et al. 1996). In the United versus long type); no more than 10% defective
States, most nuts are mature during or by the end nuts, provided that no more than 5% are poorly
of August but may be prevented from dropping by filled (blanks), no more than 5% are rancid,
an immature, clasping husk. In production areas decayed, moldy or insect injured, and no more
where hand harvesting from trees is still practiced, than 3% are insect injured; and not more than
or for varieties in which husks fail to open and 15% filberts that fail to meet the specified size
allow nut drop, nuts are considered mature and of which no more than 10% are undersized.
when they will rattle inside the husk, indicating Furthermore, shells are free from surface moisture
detachment of the nut from the base of the husk. and the combined shell and kernel moisture
content is not greater than 10%; shells are well
formed, unbroken, not discolored, have adhering
Grades, Sizes, and Packaging husk covering not more than 5% of the shell area
and are practically free of adhering dirt and other
U.S. standards exist for hazelnuts in the shell and foreign material.
for importation of in-shell or shelled hazelnuts,
and international UN/ECE standards exist for Size is specified in connection with the grade in
both in-shell and shelled hazelnuts. Grades are terms of minimum and maximum diameters that
primarily determined by size, degree of kernel will pass through round openings of a screen and
fill, color, and freedom from defects and foreign are specified separately for round-type varieties
material. U.S. No. 1 filberts must contain no more and long-type varieties. See following:
than 20% filberts of a different type (round shaped
______________________________________________________________________
Size Round-type Long-type
Minimum Maximum Minimum Maximum
______________________________________________________________________
in. (mm)
Jumbo 56/64 (22.2) — 47/64 (18.6) —
Large 49/64 (19.4) 56/64 (22.2) 48/64 (19.0) 44/64 (18.6)
Medium 45/64 (17.9) 49/64 (19.4) 45/64 (17.9) 34/64 (13.5)
Small — 45/64 (17.9) — 35/64 (13.9)
______________________________________________________________________
749
All hazelnuts imported into the United States in 5% of the kernels may be defective, of which not
lot quantities which exceed 115 lb (52.2 kg) net more than 3% may be moldy, rotten, rancid, or
weight must meet minimum grade standards as damaged by insects.
specified under section 8e of the Agricultural
Marketing Agreement Act. In-shell hazelnuts must Class I hazelnuts must be 14 mm or more
meet U.S. No. 1 grade and be at least medium size in diameter and be of good quality, must be
with a tolerance for insect injury of 2% or less. characteristic of the variety and/or commercial
Shelled hazelnuts must be well dried and clean; type, and may contain only slight defects that
free from foreign material, mold, rancidity, decay, do not affect the general appearance, quality, or
or insect injury; and free from serious damage keeping quality. They must contain not more than
caused by serious shriveling or other means. 6% empty nuts on a count basis; not more than 5%
Stated tolerances for shelled hazelnuts include not of the weight of in-shell nuts may contain shell
more than 0.0002% foreign material; not more defects; and not more than 8% of the kernels may
than 5% of kernels or pieces of kernels which be defective, of which not more than 5% may be
are below grade, including not more than 2% for moldy, rotten, rancid, or damaged by insects.
mold, rancidity, decay, or insect injury; and no
more than 1% with rancidity or insect injury. Class II hazelnuts may be of any size (if size is
specified, tolerances apply) and be of marketable
International grade standards are exercised at the
quality; they may contain defects provided that the
export control stage and are specified for both in-
in-shell nuts retain their essential characteristics as
shell and shelled hazelnuts. For in-shell nuts, UN/
regards general appearance, quality, and keeping
ECE classifications of Extra, Class I, and Class
quality. They must contain not more than 8%
II exist in which the shell of all nuts must be well
formed, intact, sound, and clean and the kernels empty nuts on a count basis; not more than 7%
must be sound, clean, sufficiently developed, and of the weight of in-shell nuts may contain shell
free from mold, visible damage from insects or defects; and not more than 12% of the kernels
pests, presence of live or dead insects or pests, may be defective, of which not more than 6% may
rancidity, foreign smell or taste, and blemishes. be moldy, rotten, rancid, or damaged by insects.
All hazelnuts must contain less than 12% moisture Sizing or screening is used to specify market size
and kernels not more than 7% moisture, and in 2 mm increments from 22 mm to 14 mm or
foreign material may not exceed 0.25% of the less. Sizing for nuts less than 22 mm in diameter
total in-shell weight. Sizing and statement of must include the maximum and minimum size
variety, commercial type, or shape is compulsory range, mention the minimum size followed by
for Extra and Class I but is optional for Class II. the words “and over,” or mention the maximum
For specified sizes in nuts of all classes, a total size followed by the words “and less.” For nuts
tolerance by count of 5% for rounded types and for final consumers under the classification
10% for oblong types for nuts not meeting the “screened,” the size “and less” is not allowed.
specified size is allowed.
For hazelnut kernels, UN/ECE classifications
Extra hazelnuts must be 16 mm in diameter of Extra, Class I, and Class II also exist; and
or more and be of superior quality, must be requirements are similar to those stated for kernels
characteristic of the variety and/or commercial of in-shell nuts with the addition of provisions
type, and must be practically free from defects for intactness of kernels and a slightly reduced
with the exception of very slight superficial tolerance for kernel moisture content from not
defects, provided they do not affect general more than 7% for in-shell kernels to not more than
appearance, quality, or keeping quality. They must 6% for shelled kernels. Foreign material may not
contain no more than 4% empty nuts on a count exceed 0.25% for kernels in any classification.
basis; not more than 3% of the weight of in-shell Sizing and statement of variety is also compulsory
nuts may contain shell defects; and not more than for Extra and Class I kernels but optional for Class
750
II. For kernels in all classes, a total tolerance of characteristics as regards general appearance,
10% is allowed for kernels other than the specified quality, and keeping quality. Total tolerances for
variety and/or type, and a size tolerance by weight defects are 16 to 18% if the lot is specified as
of 5% is allowed for rounded types and 10% for “Old Crop.” Of the total tolerance, no more than
oblong types. To be designated either Extra or 3% may be rancid, rotten, moldy, have off odor
Class I, hazelnut kernels must have a minimum or off flavor, or be damaged by insects or rodents;
diameter of 9 mm, with the exception of hazelnuts no more than 8% may be not fully developed,
of the piccolo type or hazelnuts having a similar including shrunken and shriveled, stained, and
designation for which a diameter of 6 to 9 mm is yellowish kernels; and not more than 10% may be
allowed. mechanically damaged and pieces. Not included
in the total tolerance is that not more than 8% may
Extra hazelnut kernels must be of superior be twin hazelnuts.
quality, must be characteristic of the variety and/
or commercial type, and must be practically Hazelnut kernels may be sized or screened,
free of defects with the exception of very slight with the size expressed in increments of 2 mm.
superficial defects provided they do not affect the All sizes are allowed, subject to the minimum
general appearance, quality, or keeping quality. designations for Extra and Class I hazelnut
Total tolerances for defects are 5% by weight or kernels. For kernels for final consumers under the
6% if the lot is specified as “Old Crop.” Of the classification “screened,” the size “and less” is not
total tolerance, no more than 1% may be rancid, allowed.
rotten, moldy, have off odor or off flavor, or be
damaged by insects or rodents; no more than 2%
may be not fully developed, including shrunken Optimum Storage Conditions
and shriveled, stained, and yellowish kernels; and
not more than 3% may be mechanically damaged Soon after harvest, nuts should be dried to below
and pieces. Not included in the total tolerance is 10 to 12% moisture, with kernels below 6 to
that not more than 2% may be twin hazelnuts. 7% moisture to deter mold growth. In-shell and
unroasted kernels may be stored for 24 mo with
Class I hazelnut kernels must be of good quality, minimal loss in quality at temperatures up to 10
must be characteristic of the variety and/or °C (50 °F). Roasted kernels may only be held
commercial type, and may have slight defects of for 6 mo prior to development of detectable
form and color provided that these do not affect rancidity stored at 0, 5, or 10 °C (32, 41, or 50 °F).
the general appearance, quality, or keeping quality. However, reduced temperature may be effective in
Total tolerances for defects are 12 to 13% if the lot combination with other protective measures such
is specified as “Old Crop.” Of the total tolerance, as vacuum packaging in extending roasted kernel
no more than 1.5% may be rancid, rotten, moldy, shelf-life to 1 year or more (Ebraheim et al. 1994).
have off odor or off flavor, or be damaged by
insects or rodents; no more than 4% may be not
fully developed, including shrunken and shriveled, Retail Outlet Display Considerations
stained, and yellowish kernels; and not more than
8% may be mechanically damaged and pieces. Hazelnuts are normally marketed at ambient
Not included in the total tolerance is that not more temperature. Use of vacuum or low-O2 MAP
than 5% may be twin hazelnuts. is recommended to extend shelf-life. In-shell
hazelnuts may be marketed in bulk containers.
Class II hazelnut kernels may be of any size (if Exposure to moisture and high RH should be
size is specified, tolerances apply) and be of avoided.
marketable quality; they may contain defects
provided that kernels retain their essential
751
Chilling Sensitivity pathogen associated with kernel tip mold
(Ebraheim et al. 1997, p. 483). Although
Hazelnuts are not sensitive to chilling and are Romularia spp. appear to infect hazelnuts during
commonly stored at temperatures at or below nut development and may be quiescent prior
freezing for long term storage. to maturity and storage, many molds require
breakage of the shell to contaminate the nut; thus,
intactness of the shell offers some natural defense
Ethylene Production and Sensitivity against mold infestation.
Ethylene may be used as a harvest aide to enhance The dominant flora during storage are Penicillium
maturation of husks for earlier harvest (Lagerstedt and Aspergillus spp. A. flavus, capable of
1979). During storage hazelnuts produce very low producing aflatoxin, has been isolated from
levels of ethylene. hazelnuts in storage (Eke and Goktan 1987).
Reduction of in-shell moisture content to below
10% and nutmeat moisture content to less than 6%
Respiration Rates is an effective means of deterring mold growth.
Sanitation with chlorine dips may also be effective
Properly dried hazelnuts exhibit very low in reducing the incidence of mold infestation by
respiration rates during storage. reducing the amount of innoculum carried into
postharvest storage. Because of the high amounts
of organic material on the surface of shells,
Physiological Disorders chlorine concentrations should be monitored and
replenished as necessary to maintain chlorine at
Black tips on kernels appears to be associated with concentrations necessary to kill microorganisms.
nuts having split or weak sutures. It appears to be
caused by an oxidation process that occurs on the
pellicle only and may or may not be associated Quarantine Issues
with moldy kernels (Thompson et al. 1996).
To prevent spread of eastern filbert blight
Twin kernels occur when two kernels develop (Anisogramma anomala), all trees, plants,
within one nut shell, and is an undesirable trait cuttings, and scions of all species and varieties
because of the small size and irregular shape of of the wild and cultivated filbert or hazelnut,
affected kernels. Corylus spp., may not be transported into Oregon
from States and districts of the U.S. east of and
Blank nuts are in-shell hazelnuts devoid of normal including the States of Montana, Wyoming,
kernels. It results from defective embryo sacs, Colorado, and New Mexico; the entire State of
unviable eggs, failure of fertilization, or embryo Washington; and all provinces, districts, and
abortion at varying stages of development. Owing territories of Canada east of and including the
to the alternate bearing cycles for hazelnuts, province of Alberta.
poorly filled nuts may also result during an
overproduction year, and poor kernel fill appears
to be a heritable trait (Thompson et al. 1996). Special Considerations
752
An ELISA test can be used to detect hazelnut Mehlenbacher, S.A., and J. Olsen. 1997. The
presence in complex food mixtures (Holzhauser hazelnut industry in Oregon, USA. Acta Hort.
and Vieths 1999). Shelling, blanching, or roasting 445:337-346.
decreases hazelnut shelf-life compared to
storage in-shell (Ebraheim et al. 1994). Roasting Richardson, D.G. 1997. The health benefits of
temperature and duration interact to decrease eating hazelnuts: implications for blood lipid
shelf-life with increasing roasting temperature profiles, coronary heart disease and cancer risks.
and duration (Richardson and Ebraheim 1997). Acta Hort. 445:295-300.
Hazelnuts absorb lipophilic compounds that can
induce off flavor. Although frozen storage may Richardson, D.G., and K. Ebraheim. 1997.
be used to increase shelf-life, once out of storage Hazelnut kernel quality as affected by roasting
hazelnuts should be used as soon as possible due temperatures and duration. Acta Hort. 445:301-
to reduced shelf-life at room temperature. 304.
753
Macadamia Nut Shortly after this time, the nuts fall to the
ground and are harvested. Sometimes shake-
Catherine G. Cavaletto harvesting is employed to facilitate the harvest
while minimizing the amount of immature nuts
Cavaletto is with the Department of Tropical Plant harvested. Recommended harvest interval for
and Soil Science, University of Hawaii at Manoa, ground-harvested nuts is 1 mo. Damage by the
Honolulu, HI. tropical nut borer (Hypothenemus obscurus [F.])
can begin to increase rapidly after 3 weeks on the
ground (Jones 1992).
Scientific Name and Introduction
The family Proteaceae includes about 10 species Grades, Sizes, and Packaging
of the genus Macadamia, 2 of which produce
edible nuts: M. integrifolia and M. tetraphylla or No U.S. grades are established for macadamia
hybrids of these. The major species of commerce nuts, but the State of Hawaii has standards and
is M. integrifolia. Macadamia nuts are native to grades for shelled macadamia nuts, in-shell
Australia and are produced there as well as in macadamia nuts, and roasted macadamia nuts
Hawaii, Central and South America, and parts of (Hawaii Department of Agriculture 1984).
Africa. Nearly all production consists of grafted The minimum export grade for shelled nuts is
trees of cultivars developed in Hawaii or Australia. Hawaii No. 1, which defines defects and damage,
The edible kernel is enclosed in a thick, hard shell development, and cleanliness as well as maximum
that, in turn, is enclosed in a husk that separates moisture content of 1.5% by weight. Off-grade is
from the tree at about the time the seed is mature. not a grade, but is a descriptive term applicable
The kernel is nearly spherical, consisting of joined to shelled nuts which have a market value
equal-sized halves (cotyledons). and designates a quality lower than the lowest
applicable Hawaii grade for shelled nuts. The
standards also define eight styles: Style I (wholes),
Quality Characteristics and Criteria Style II (wholes and halves), Style III (cocktail),
Style IV (halves and pieces), Style V (large diced),
Kernels should be a light cream color, nearly Style VI (chips), Style VII (bits, diced), and Style
spherical in shape, and free of blemishes, VIII (fines). There are also standards for in-shell
rancidity, mold, decay, insect damage, extraneous nuts and for roasted kernels. Shelled nuts are
material, hollow centers, discoloration, and usually packaged in 11.4 to 22.7 kg (25 to 50 lb)
adhering shell. Fully developed macadamia vacuum-packed or nitrogen-flushed foil bags for
kernels should contain ≥72% oil; the highest the wholesale market. Larger nitrogen-flushed
quality kernels contain 72 to 78% oil and 1.5% containers are sometimes used.
moisture. Kernels of M. tetraphylla typically
contain less oil. Oil content is a major quality
factor that varies with cultivar and maturity and is Optimum Storage Conditions
inversely related to sugar content (Ripperton et al.
1938). Macadamia nuts should be husked within 24 h of
harvest, after which the drying process should be
initiated. Freshly fallen nuts contain about 25%
Horticultural Maturity Indices kernel moisture, although nuts that have remained
on the ground for extended periods may have as
Following a prolonged flowering season, little as 10 to 15% moisture. Drying should begin
macadamia nuts mature over a long period with ambient air, followed by a gradual increase
from late summer until late spring. Kernels in temperature that will not exceed 60 °C (140
are mature when oil accumulation is complete. °F) in the final stage of drying. Drying may be
754
completed in-shell (to 1.5% kernel moisture); or begin the drying process at ambient temperature
the nuts can be dried partially in-shell (to about 5 or with circulating air heated to no more than
to 6% kernel moisture), followed by cracking and 38 °C (100 °F) when the kernels are very wet.
finish drying of kernels alone to 1.5% moisture. Minimum reducing-sugar levels can be obtained
It is important to protect the dry kernels from if kernel moisture content is reduced to 8% before
moisture and O2, so packaging in a material that increasing the temperature to 52 °C (126 °F) and
is impervious to moisture is important. Vacuum to 6% before increasing the temperature to 60 °C
packaging or nitrogen flush offers protection (140 °F) (Prichavudhi and Yamamoto 1965).
from O2. Exposure to moisture results in loss
of crispness and shelf-life. Likewise, prolonged
exposure to O2 results in rancidity. Cold storage is Postharvest Pathology
normally not necessary for short-term storage but
might be desirable for extended periods. Frozen Extended harvest intervals may result in mold,
storage (-18 °C, 0 °F) can be very effective in yeast, or bacterial contamination in the field, as
extending shelf-life. well as insect damage. Southern green stinkbug
(Nezara viridula [L.]), tropical nut borer
(Hypothenemus obscurus [F.]) koa seed worm
Retail Outlet Display Considerations (Cryptophlebia illepida [Butler]), and litchi fruit
moth (C. ombrodelta) are the major insect pests
Packaging should protect kernels from moisture causing damage to macadamia nuts in Hawaii,
and O2. Storage temperature is less critical. resulting in an unmarketable kernel (Jones and
Nuts are rarely sold in-shell due to difficulty in Aihara-Sasaki 1999). Delay in husking the nuts
cracking. following harvest can also result in mold growth
and fermentation. Husking should occur within 24
h of harvest. The thick shell provides considerable
Chilling Sensitivity protection for the kernel, but cracked shells and
open micropyles can provide entry of fungi and
Macadamia nuts are not sensitive to chilling bacteria as well as ants. When the nuts fall from
provided they have been dried. Dry kernels the tree, the kernels contain about 25% moisture.
are commonly stored below 15 °C (59 °F) for At harvest, moisture level can range from about
bulk storage and can successfully be frozen to 10 to 25%. Once the nuts have been dried to 1.5%
extend shelf-life. Care must be taken in thawing kernel moisture, the water activity is 0.3 (Beuchat
frozen nuts to prevent condensation and resulting 1978), insufficient to support the growth of mold
moisture gain. or bacteria.
Nuts that fail to develop properly will produce There are no significant quarantine issues. A
kernels that are low in oil content and may appear possible issue is the occasional presence of storage
shriveled or small. If too immature, kernels insects such as almond moth (Ephestia cautella),
are very small and become hard upon drying. merchant grain beetle (Oryzaephilus mercatur
Immature kernels also have high sugar content [Fauvel]), and the dried fruit beetle (Carpophilus
and will brown excessively when roasted. High- hemipterus [Linnaeus]) (Cavaletto 1983).
moisture kernels dried at higher temperatures
develop brown centers when roasted as a result
of high reducing sugar levels. It is necessary to
755
Special Considerations Ripperton, J.C., R.H. Moltzau, and D.W. Edwards.
1938. Methods of evaluating the macadamia nut
Kernel stability is related to moisture content for commercial use and the variation occurring
and to oxidative rancidity. Of these, moisture among seedling plantings in Hawaii. Bull.
content is the most critical. Raw kernels with 79, Hawaii Agricultural Experiment Station.
moisture content exceeding 2% have poor Honolulu, HI.
stability (Cavaletto et al. 1966). These kernels
brown excessively when roasted and have poor
texture. Likewise, roasted kernels must be well
protected from moisture to ensure preservation of
texture and flavor. Protection from O2 is usually
accomplished with vacuum or nitrogen packing.
References
756
Peanut or foreign material and off odor or flavor. Raw
peanuts should be surrounded by a tan, pink, or
Niels Maness red seedcoat (testa) that fully encapsulates the
seed, and the interior color of each half-seed
Maness is with the Department of Horticulture should be ivory. Moisture content for in-shell
and Landscape Architecture, Oklahoma State peanuts should be <10% to prevent mold growth
University, Stillwater, OK. (Diener and Davis 1977). Prior to shelling,
peanuts should contain 7 to 10% moisture to
reduce splitting and kernel breakage during
Scientific Name and Introduction milling. After milling, moisture content for
maximum shelf-life is ≤7%. Seed may be stored
Arachis hypogaea L., the peanut or groundnut, is at ambient temperature for up to 11 years with
an annual herb of the Leguminosae family. Two good viability if seed moisture content is <3.3%
subspecies are grown commercially, and four (Cheng et al. 1997). Peanuts marketed without
market types are of greatest economic importance. seedcoats (blanched) should have an ivory-colored
A. hypogaea subsp. hypogaea includes the market raw kernel. Peanuts are most commonly consumed
types “runner” and “Virginia,” and A. hypogaea following roasting, which may be accomplished
subsp. fastigata includes the market types in-shell or after shelling. Roasted peanut kernels
“Spanish” and “Valencia.” In the United States should be light yellow in color, free of external
in the 1980s, 70% of the peanuts grown were oil, contain <6% moisture, and be free of dark-
runners, while 20, 10, and <1% were Virginia, colored kernels.
Spanish, and Valencia market types, respectively
(Knauft and Gorbet 1989). According to the Peanut seed, and particularly peanut seedcoats, are
USDA national peanut tonnage report, U.S. a source of resveratrol, a compound that reduces
production in 1999 included 78% runners, 18% cardiovascular disease and cancer incidence.
Virginia, 3% Spanish, and 3% Valencia. Resveratrol ranges from 0.02 to 1.79 μg g-1,
compared to 0.6 to 8.0 μg mL-1 in red wines
The edible portion is a seed which develops (Sanders et al. 2000). Fatty acid composition of
underground inside a pod containing two to four peanut oil is predominantly oleic and linoleic
seeds. Peanuts originated in South America and acids, found in roughly equal amounts and making
are now cultivated worldwide, with the majority up 80% of total fatty acids. Certain genotypes
of production in India, Asia, and the United may contain substantially more oleic than linoleic
States. In the United States, about 60% of peanuts acid, with ratios as high as 40:1 (oleic:linoleic).
are used in a variety of food products, with the Peanuts with a high oleic:linoleic ratio are less
reminder used in approximately equal proportions susceptible to oxidative deterioration and off
for export and for production of edible oil. flavor development caused by oxidative cleavage
Major chemical constituents of peanuts are oil of polyunsaturated fatty acids. The ratio of oleic
(44 to 56%) and protein (22 to 33%), with large to linoleic is influenced primarily by genotype,
influences by environment, genotype, and maturity but interactions exist between genotype and the
on their concentrations (Holaday and Pearson environment.
1974).
Horticultural Maturity Indices
Quality Characteristics and Criteria
Peanuts are an indeterminant plant, with flowering
Shelled peanuts should be properly sized to followed by underground seed development over
meet market type, be free of misshapen or a range of time. Assessment of peanut maturity
underdeveloped kernels, and be free of any shell should be conducted using multiple plants at
757
Table 1. Comparison of screen sizes and tolerances (by weight) for American Peanut
Shellers Association (APSA) and USDA Grades for shelled Spanish peanuts
Grade and minimum Fall-through Other Splits Damage Damage Foreign Moisture
screen size prescribed types and minor material
screens defects
%
* (APSA) 2.00% sound portions of peanuts which pass through the prescribed screen; and 4.00%
for sound whole kernels, not over 3.00% which will pass through a 13/64×3/4 in screen. This fall-
through combined with percentage of sound portions shall not exceed 4.00%.
** (US) 2.00% sound portions of peanuts which will pass through the prescribed screen; and 4.00% for
sound whole kernels.
*** Combined fall-through of sound portions through a 16/64 in round screen and sound whole kernels
through a 13/64×3/4 in screen shall not exceed 4.00%
758
Table 2. Comparison of screen sizes and tolerances (by weight) for American Peanut Shellers Association
(APSA) and USDA Grades for shelled runner peanuts
Grade and Fall-through Fall-through Other Splits Damage Damage Foreign Moisture
minimum prescribed 16/64 x 3/4” types and minor material
screen size screens slot screen defects
%
Jumbo
38-42 ct. per oz.***
or 21/64 x 3/4” slot 5.00 3.00 1.00 3.00 1.00 2.00 0.10 9.00
Medium
40-50 ct. per oz.***
or 18/64 x 3/4” slot
(Report % riding
21/64 x 3/4” slot) 5.00 3.00 1.00 3.00 1.00 2.00 0.10 9.00
Select
16/64 x 3/4” slot
(Report % riding
21/64 x 3/4” slot) ---- 3.00 1.00 3.00 1.00 2.00 0.10 9.00
No. 1
16/64 x 3/4” slot
(Report % riding
18/64 x 3/4” slot)
APSA ---- 3.00 1.00 3.00 1.00 2.00 0.10 9.00
US ---- 3.00 1.00 3.00 1.50 2.00 0.10 ----
Mill run
16/64 x 3/4” slot ---- 3.00 1.00 3.00 1.00 2.00 0.10 9.00
759
Table 2. Comparison of screen sizes and tolerances (by weight) for American Peanut Shellers Association
760
(APSA) and USDA Grades for shelled runner peanuts—continued
Grade and Fall-through Fall-through Other Splits Damage Damage Foreign Moisture
minimum prescribed 16/64 x 3/4” types and minor material
screen size screens slot screen defects
%
Splits
17/64” round
APSA * * 2.00
----
1.50 2.00 0.20 9.00
US ** ** 2.00 ----
2.00 2.00 0.20 ----
* (APSA) 2.00% sound portions pass through 17/64 in round screen. 4.00% for sound whole kernels, not over 3.00% which
will pass through 14/64×3/4 in slot screen. This fall-through combined with sound portions passing 17/64 in round not to
exceed 4.00%.
** (US) 2.00% sound portions pass through 17/64 in round. 4.00% for sound whole kernels.
*** American count per ounce shall be within the specified range.
Each of the above grades may be certified “with splits” providing all requirements of the grade are met, except that a tolerance of
15% is allowed for split kernels of which not more than 3% will pass through 17/64 in round screen.
Table 3. Comparison of screen sizes and tolerances (by weight) for American Peanut Shellers Association (APSA)
and USDA Grades for shelled Virginia peanuts
Grade and Fall-through Other Sound Split Damage Damage Foreign Moisture Count per
minimum prescribed types or broken and minor material pound
screen size screens kernels defects maximum
%
Extra large
20/64 x 1” slot
APSA & US 3.00 0.75 3.00 1.00 1.75 0.10 ---- **512
Medium Virginia
18/64 x 1” slot
APSA & US 3.00 1.00 3.00 1.25 2.00 0.10 ---- **640
No. 1 Virginia
15/64 x 1” slot
APSA & US 3.00 1.00 3.00 1.25 2.00 0.10 ---- **864
Virginia splits
20/64” round
APSA & US *3.00 2.00 Not less ---- 2.00 0.20 ---- ----
than 90.00
No. 2 Virginia
17/64” round
APSA & US *6.00 2.00 As graded ---- 2.50 0.20 ---- ----
* (APSA & US)—includes both sound split and broken and sound whole kernels which pass through prescribed screens.
** Unless otherwise specified.
Each of the above APSA whole grades may be certified as “with splits” providing all requirements of the grade are met, except that a
tolerance of 15% is allowed for split kernels of which not more than 3% will pass through 17/64 in round screen.
761
Table 4. Comparison of screen sizes and tolerances (by weight) for American Peanut
Shellers Association (APSA) and USDA Grades for in-shell Virginia peanuts
Jumbo
37/64 x 3”
APSA 5.00 *10.00 3.50 **176 (U.O.S.)
US 5.00 *10.00 3.50 **176 (U.O.S.)
Fancy
32/64 x 3”
APSA 5.00 *11.00 4.50 **225 (U.O.S.)
US 5.00 *11.00 4.50 **225 (U.O.S.)
* (APSA & US)—not more than 0.5% shall be allowed as dirt or other foreign material.
** Unless otherwise specified.
762
various locations within a field. Peanut maturity damage and concealed damage from rancidity,
may be judged by the shell-out procedure, mold, or decay. Segregation 1 peanuts may
involving separation of peanut seed into mature not contain more than 2.00% damaged kernels
or immature categories. Using the shell-out nor more than 1.00% concealed damage, while
procedure, a peanut is considered mature if the segregation 2 peanuts may contain more than
inner hull is brown and the seed coat is pink to 2.00% damaged or more than 1.00% concealed
red. Optimum maturity is reached for runner damage. Segregation 3 peanuts are those which
and Spanish types when 75 to 80% of the inner contain visible Aspergillus flavus mold or 15 ppb
hull has turned brown and for Virginia types (nL L-1) or more aflatoxin.
when 65% of the seed coat has turned deep pink.
Runner peanut maturity can be determined by a
hull-scrape method, in which maturity profiles for Optimum Storage Conditions
samples are estimated based on degree of change
in pod mesocarp from white to brown to black In-shell farmers-stock peanuts should be dried
(Williams and Drexler 1981). to about 7.5% moisture. If stored at 10 °C (50
°F), these can be stored for up to 10 mo without
significant quality loss (Davidson et al. 1982).
Grades, Sizes, and Packaging High losses in milling quality may occur if
peanuts are dried to below 7% moisture or if
U.S. grade standards and industry grade standards kernel temperature is below 7 °C (44.6 ºF) during
from the American Peanut Shellers Association shelling (McIntosh and Davidson 1971). Peanut
exist for shelled Spanish, shelled runner, moisture content >10% should be avoided to
shelled Virginia, and in-shell Virginia peanuts. prevent mold growth (Diener and Davis 1977).
A comparison of tolerances as provided by the Adequate ventilation in a warehouse storage
American Peanut Shellers Association fficial facility, preferably providing one air change every
Trade Rules for shelled peanuts based primarily 3 min, is also desirable to prevent excess moisture
on size, peanuts of other types, amount of split or and heat from accumulating in the storage facility
broken kernels, freedom from foreign material, (Smith and Davidson 1982).
damage, minor defects, and in some cases
moisture percentage are presented in tables 1 to Quality of raw shelled peanuts can be maintained
4. Tolerances for in-shell Virginia peanuts based for at least 1 year at 1 to 5 °C (34 to 41 °F) with
on maturity; freedom from loose shelled peanuts; moisture content of <7%, or for 2 to 10 years at
discoloration of shell; presence of dirt, shell, and -18 °C (0 °F) and <6 % moisture. Maintaining
other foreign material; and degree of kernel fill RH between 55 and 70% at 1 to 5 °C (34 to 41
inside the shell are compared. °C) will maintain peanut moisture content at 7 to
7.5%. Careful handling of peanuts equilibrated
Raw in-shell peanuts are typically stored to <5 °C (41 °F) is necessary to prevent bruising
as “farmer stock peanuts” in flat, ventilated and subsequent oil seepage from damaged
warehouses or grain bins in bulk or, less cells within the cotyledon. Upon removal of
commonly, in 50-lb (23 kg) burlap bags for a raw shelled peanuts from refrigerated or frozen
week to 10 mo prior to shelling (Smith et al. storage, equilibration to ambient temperature
1995). After shelling, raw peanuts are often should be gradual in conditioning rooms, with
shipped in bulk containers but may be packaged in RH, temperature, and air-flow adjusted to prevent
burlap or nylon tote bags of various sizes. Peanuts moisture condensation onto peanuts.
for human consumption must be free of visible
Aspergillus flavus mold, containing less than 15
ppb (nL L-1) aflatoxin. Lots imported into the
United States may be designated “segregation 1”
or “segregation 2” depending on degree of kernel
763
Controlled Atmosphere Considerations storage. Soaking seeds in GA3 or ethephon at
50 to 200 μg mL-1 was effective in breaking
Low-O2 storage shows promise for delaying dormancy (Kapur et al. 1990). Nondormant
rancid flavor development and insect infestation peanuts exhibit a climacteric-like rise in ethylene
(Slay et al. 1985). High-CO2 storage appears production during seed germination (Whitehead
to limit growth of Aspergillus flavus in short- and Nelson 1992).
duration storage of high-moisture, noncured
peanuts. Peanuts at 20% moisture stored at 0.6
to 3 °C (33 to 37 °F) in a high-CO2 environment Respiration Rates
had acceptable quality for 4 days but deteriorated
after 8 days of storage (Moseley et al. 1971). For Properly cured peanuts in storage exhibit a
longer-term storage of high-moisture, shelled relatively low rate of respiration. During harvest
peanuts under ambient temperature conditions, and prior to curing, especially for high-moisture
<1.5% O2 was required to slow Aspergillus flavus peanuts, respiration rates may be substantial and
growth; but no CA totally eliminated aflatoxin significant losses in quality can ensue. Freshly
production (Wilson and Jay 1976). harvested peanuts should be dried soon after
harvest to <10% moisture to assure optimum
quality.
Retail Outlet Display Considerations
764
presence of toxic fungal metabolites (mycotoxins) virus. During import of peanuts, all lots must
are a particular concern. The name “aflatoxin” be labeled with a positive lot identification and
refers to four metabolites found in contaminated must meet the requirements for segregation 1
peanuts and designated aflatoxin B1, B2, G1, peanuts if used for human consumption. Peanuts
and G2. Aflatoxins B1 and B2 are metabolites of with visible Aspergillus flavus mold, or those
Aspergillus flavus, and all four aflatoxins may be containing ≥15 ppb aflatoxin, may not be used for
produced by Aspergillus parasiticus (Cole et al. edible purposes; they may be used for oil stock.
1995). A fifth mycotoxin, cyclopiazonic acid, is Such peanuts may be blanched and reseparated
somewhat less toxic than aflatoxin and is produced into aflatoxin-negative lots, which may be used for
by Aspergillus flavus, other Aspergillus species, edible purposes.
and several species of Penicillium (Dorner et al.
1985).
Special Considerations
Preharvest conditions favoring aflatoxin
contamination are high temperatures and Peanuts are a major allergenic food among adults
drought stress during the last 3 to 6 weeks of and children in the United States (Taylor 1992).
the growing season (Cole et al. 1989). Late- Allergen activity has been identified for at least
season irrigation may be effective in reducing six allergens by phage display technology and is
aflatoxin contamination (Dorner et al. 1989). known to be in association with the two major
When aflatoxin contamination occurs, it is storage proteins, arachin and conarachin, and in
common for most of the harvested seed to be profilin (Kleber et al. 1999). Allergens may also
free of contamination with only a few highly be present in refined peanut oil (Olszewski et al.
contaminated seeds. Although monitoring at the 1998). Due in large part to allergenicity, any food
point of sale for Aspergillus flavus is mandated product containing peanuts or peanut oil must be
by the USDA Peanut Marketing Agreement, and labeled as such.
detection of ≥15 ppb (nL L-1) aflatoxin leads to
positive aflatoxin identification, the irregular Careful handling of cured farmers-stock peanuts
distribution of infection may lead to false negative to prevent breakage of shells reduces the risk for
designations. spread of fungal contamination and maintains
the grade. Conveyors, cleaners, sizers, and
Storage conditions to deter growth of the causal other handling equipment should be padded
organisms and subsequent metabolic production where appropriate and properly maintained to
of the mycotoxins primarily involve prevention prevent excessive breakage during handling.
of rehydration during storage. Decontamination Once shelled and roasted, peanuts should be
of contaminated lots is most effectively done with handled carefully to prevent separation of the
electronic color sorting, although size and density half kernels and breakage since splits and pieces
separation may also be effective in removal of the are more susceptible to oxidative deterioration
most susceptible underdeveloped seed. and rancidity development. Peanuts will absorb
lipophilic volatiles from their surroundings or
from inappropriate packaging that can induce
Quarantine Issues off flavors. Absorption of ammonia can cause
darkening of nutmeats.
Importation of peanut seed into the United States
for planting is prohibited from Burkino Faso,
the People’s Republic of China, Cote d’Ivoire,
India, Indonesia, Japan, the Phillipines, Senegal,
Thailand, and Taiwan because of peanut stripe
765
References
Holaday, C.E., and J.L. Pearson. 1974. Effects of
Cheng, H.Y., G.H. Zheng, X.M. Jing, et al. 1997. genotype and production area on the fatty acid
Storage of peanut seeds with low moisture content composition, total oil and total protein in peanuts.
for 11 years in ambient temperature. Plant Gen. J. Food Sci. 39:1206-1209.
Resour. Newsletter 110:35-40.
Jakkula, L.R., S.F. O’Keefe, D.A. Knauft, and
Cole, R.J., J.W. Dorner, and C.C. Holbrook. K.J. Boote. 1997. Chemical characterization of a
1995. Advances in mycotoxin elimination and shriveled seed trait in peanut. Crop Sci. 37:1560-
resistance. In H.E. Pattee and H.T. Stalker, 1567.
eds., Advances in Peanut Science, pp. 456-474.
American Peanut Research and Education Society, Kapur, A., J. Kaur, H.L. Sharma, and H. Singh.
Stillwater, OK. 1990. Preconditioning of peanut (Arachis
hypogaea) seeds to release dormancy. Ann. Biol.
Cole, R.J., T.H. Sanders, J.W. Dorner, and P.D. Ludhiana 6:141-145.
Blankenship. 1989. Environmental conditions
required to induce preharvest aflatoxin Kleber, J.T., R. Crameri, U. Appenzeller, et al.
contamination of groundnuts: summary of 1999. Selective cloning of peanut allergens,
six years research. In Proceedings of the including profilin and 2S albumins, by phage
ICRISAT International Workshop on Aflatoxin display technology. Int. Arch. Allergy Immun.
Contamination of Groundnut, 1989, pp. 279-287. 119:265-274.
International Crops Research Institute for the
Semi-Arid Tropics Center, Patancheru, Peru. Knauft, D.A., and D.W. Gorbet. 1989. Genetic
diversity among peanut cultivars. Crop Sci.
Davidson, J.I., T.B. Whitaker, and J.W. Dickens. 29:1417-1422.
1982. Grading, cleaning, storage, shelling and
marketing of peanuts in the United States. In McIntosh, F.P., and J.I. Davidson. 1971. Effect
H.E. Pattee and C.T. Young, eds., Peanut Science of temperature on shelling runner- and spanish-
and Technology, pp. 571-623. American Peanut type peanuts. Rep. 52-65, U.S. Department of
Research and Education Society, Yoakum, TX. Agriculture, Agricultural Research Service,
Washington, DC.
Diener, U.L., and N.D. Davis. 1977. Aflatoxin
formation in peanuts by Aspergillus flavus. Bull. Moseley, Y.C., H.B. Manbeck, G.L. Barnes, and
493, Auburn University Agricultural Experiment G.L. Nelson. 1971. Controlled atmosphere for
Station, Auburn, AL. short duration storage of peanuts before drying.
Trans. Amer. Soc. Agr. Eng. 14:206-210.
Dorner, J.W., R.J. Cole, and L.G. Lomax. 1985.
The toxicity of cyclopiazonic acid. In J. Lacey, Olszewski, A., L. Pons, F. Moutete, et al. 1998.
ed., Trichothecenes and Other Mycotoxins, pp. Isolation and characterization of proteic allergens
529-535. John Wiley and Sons, New York, NY. in refined peanut oil. Clin. Exper. Allergy 28:850-
859.
Dorner, J.W., R.J. Cole, T.H. Sanders, and P.D.
Blankenship. 1989. Interrelationship of kernel Sanders, T.H., P.B. Blankenship, J.R. Vercellotti,
water activity, soil temperature, maturity and and K.L. Crippen. 1990. Interaction of curing
phytoalexin production in pre-harvest aflatoxin temperature and inherent maturity distribution in
accumulation of drought-stressed peanuts. descriptive flavor of commercial grade sizes of
Mycopathalogia 105:117-128. Florunner peanuts. Peanut Sci. 17:85-89.
766
Sanders, T.H., R.W. McMichael, and K.W. Wilson, D.A., and E. Jay. 1976. Effect of
Hendrix. 2000. Occurrence of resveratrol in edible controlled atmosphere storage on aflatoxin
peanuts. J. Agric. Food Chem. 48:1243-1246. production in high moisture peanuts (groundnuts).
J. Stored Prod. Res. 12:97-100.
Sanders, T.H., J.R. Verecellotti, P.B. Blankenship,
et al. 1989. Interaction of maturity and curing
temperature on descriptive flavor of peanuts. J.
Food Sci. 54:1066-1069.
767
Pecan Horticultural Maturity Indices
Carya illinoinsis L. is a member of the hickory In-shell and shelled grades exist and are primarily
family (Jugulaceae). The edible seed is surrounded determined by size, degree of kernel fill, color,
by a shuck that opens (dehisces) when the seed is and freedom from defects and foreign material.
mature. Nuts are harvested in late fall and early Grades for in-shell pecans include U.S. No. 1 and
winter by mechanically shaking the trees and U.S. No. 2, based primarily on uniformity of shell
gathering the nuts from the orchard floor. Nuts color, absence of shell defects, kernel defects, and
are covered by a shell that is divided into two extraneous or foreign material. Sizes of in-shell
relatively equal-sized halves. Pecans are native pecans are based on number of nuts per 0.45 kg (1
to the southern United States and Mexico and lb) and include Oversize (≤55), Extra Large (56
are classified for marketing either as seedling to 63), Large (64 to 77), Medium (78 to 95), and
(primarily natives) or cultivars. Seedling pecans Small (96 to 120). In-shell pecans are packaged in
have typically thicker shells with smaller kernels, bags containing 36.4 to 54.5 kg (80 to 120 lb) or
lower shellout percentage, and higher oil content in palletized containers.
than cultivars. The majority of commercial
production is in cultivars such as ‘Western,’ Grades for shelled pecans include U.S. No. 1 and
‘Desirable,’ ‘Stuart,’ ‘Wichita,’ and ‘Pawnee.’ U.S. Commercial for halves, halves and pieces,
and pieces based primarily on testa color, degree
of kernel fill, moisture content, freedom from shell
Quality Characteristics and Criteria or extraneous material, and freedom from damage.
When the color of kernels in a lot conforms to
Pecan kernels should be yellow-golden to light- the “light” or “light amber” classification, these
brown in color, be of roughly equal width from color designations may be used to describe the lot
one end to the other, and be free of any shell or in connection with the grade. Size classifications
other foreign material as well as off odor or flavor. for halves are based on number of halves per 0.45
Water content should be <4% to prevent mold kg (1 lb) and include Mammoth (≤250), Junior
growth and retard rancidity development. Water mammoth (251 to 300), Jumbo (301 to 350), Extra
content <2% increases brittleness that induces large (351 to 450), Large (451 to 550), Medium
greater kernel breakage during handling and (551 to 650), Small or Topper (651 to 750), and
enhances cracking of the testa, which increases O2 Midget (≥751). Size classification for pieces are
permeation of the nutmeat, favoring rancidity. Oil based on the maximum and minimum round
content of pecan kernels inversely affects shelf- diameter in inches through which the pieces will
life, and oil content varies from 55 to 75% (by or will not pass and include Mammoth (>1.3 cm,
weight) and is influenced by cultivar, geographic 1/2 in), Extra large (1.4 to 1.1 cm, 9/16 to 7/16 in),
location, and year of production (Wells and Halves and pieces (>0.8 cm, 5/16 in), Large (1.3
McMeans 1978). to 0.8 cm, 1/2 to 5/16 in), Medium (1.0 to 0.5 cm,
3/8 to 3/16 in), Small (0.6 to 0.3 cm, 1/4 to 1/8
in), Midget (0.5 to 0.2 cm, 3/16 to 1/16 in), and
Granules (0.3 to 0.2 cm, 1/8 to 1/16 in). Shelled
768
pecans are packaged in 13.6-kg (30-lb) grease- h (Dull and Kays 1988). Vacuum packaging can
proof boxes, either loose or vacuum-sealed inside offer a further benefit of protection from breakage.
plastic bags.
769
maturity, consume the nutmeat, and exit the pecan E. coli is a common shell contaminant, especially
leaving a small round hole in the shell. Pecans for nuts harvested in orchards where animal
containing pecan weevil larvae can sometimes be grazing is practiced (Marcus and Amling 1973).
identified by an oil-soaked or reddish appearance. Contamination of nutmeats may occur if shells are
broken during harvest or if shells are not properly
Opalescence, a condition characterized by sanitized by addition of chlorine to heated soak-
an opaque or oil-stained appearance of all or water just prior to cracking. Because of high
a portion of the nutmeat, has been identified amounts of organic material on the surface of
and attributed to breakdown of oil bodies and pecan shells, chlorine should be monitored and
subsequent leakage of oil within the cotyledon. replenished as needed to maintain the desired
This leakage increases O2 exposure of oil and concentration.
decreases shelf-life by accelerating oxidative
rancidity development.
Quarantine Issues
Sticktights are nuts that fail to shed the shuck at
harvest. Insufficient moisture in fall, or insect Pecan shucks, shells, and nuts in the shell, as
feeding on the shuck, may cause this condition well as containers, equipment, and vehicles used
because shuck splitting is an active process that in association with them, must be free of pecan
requires the shucks to remain turgid and for casebearer, pecan weevil, and hickory shuckworm
abscission layers to form at the sutures and at prior to transportation into California.
the peduncle (Worley 1982). Early fall freeze
or potassium insufficiency may also increase
prevalence of sticktights. Kernels from sticktight Special Considerations
pecans are often of lower quality due to premature
death of the vascular system feeding the Pecans marketed in-shell should be cracked and
developing kernels. sampled periodically to assess nutmeat quality.
Pecan halves and pieces marketed in plastic
Vivipary (sprouting of nuts on the tree) renders bags should be handled with care to prevent
affected pecans unmarketable and may be reduced excessive breakage and grade change. Since
by late season irrigation (Stein et al. 1989) or by most bags for marketing shelled pecans are clear,
fruit thinning (Sparks et al. 1995). care in prevention of exposure to direct sunlight
or excessive UV radiation should be exercised
to prevent darkening of the testa. Increased
Postharvest Pathology temperature during handling can also enhance
darkening and promote development of rancid
The most common decay found in pecans is flavor. Pecans will absorb lipophilic volatiles in
caused by molds, with Penicillium and Aspergillus the environment that can introduce off flavors.
most prevalent (Huang and Hanlin 1975). The Absorption of ammonia will cause darkening of
intact shell provides some defense against mold. nutmeats. Although frozen storage increases shelf-
Some strains of A. flavus and A. parasiticus that life, once out of storage pecans should be used
have been isolated from pecans can produce quickly because of their short shelf-life at room
aflatoxins, a heat-resistant carcinogenic and temperature compared to never-frozen pecans.
toxic byproduct. Storage at refrigerated or frozen
temperatures prevents or slows mold growth.
Reduction of nutmeat moisture content to <4.5%
and storage at RH <70% provide a water activity
of 0.65 to 0.70, which will not support growth of
most molds.
770
References Wells, J.M., and J.L. McMeans. 1978. Rancidity,
iodine values and oil content of 21 pecan cultivars.
Dull, G.G., and S.J. Kays. 1988. Quality and Proc. Southeastern Pecan Growers’ Assoc. 71:159-
mechanical stability of pecan kernels with 162.
different packaging protocols. J. Food Sci. 53:565-
567. Woodroof, J.G., and E.K. Heaton. 1962. Storage
of pecans. Research Bull. 149, University of
Eddy, M., and J.B. Storey. 1988. The influence Georgia, Georgia Experiment Station, Athens, GA.
of harvest date on oil and flavor development
in Desirable and Whichita pecans. HortScience Worley, R.E. 1982. Tree yield and nut
23:782. characteristics of pecan with irrigation under
humid conditions. J. Amer. Soc. Hort. Sci. 107:30-
Heaton, E.K., A.L. Shewfelt, A.E. Badenhop, and 34.
L.R. Beuchat. 1977. Pecans: handling, storage,
processing and utilization. Research Bull. 197,
University of Georgia, Georgia Experiment
Station, Athens, GA.
771
Pistachio in-shell product is a shell that is free of staining.
This is not only for cosmetic reasons. Shell
John M. Labavitch staining is also an indicator of development,
pathogen, and insect problems prior to harvest.
Labavitch is with the Department of Plant Kernel quality criteria include a firm, crisp texture
Sciences, University of California, Davis, CA. (which is degraded by insufficient drying after
harvest or storage at too high an RH), a sweet
and oily flavor, and freedom from rancidity
Scientific Name and Introduction (Kader et al. 1982). Kernels are high in fat
content (approximately 45% by weight) and crude
Pistacia vera L. is the only species of the 11 in protein (approximately 30%). Total levels of low-
the genus Pistacia that produces edible nuts. It is molecular-weight sugars are 3 to 4%, but reducing
a native of western Asia and Asia Minor, and wild sugars (primarily glucose and fructose) make up
representatives are still found in hot, dry locations only about 10% of the total sugar (Kader et al.
in these areas. The pistachio was introduced to 1982, Labavitch et al. 1982).
Europe at the beginning of the Christian Era.
The USDA plant exploration service introduced
the pistachio to the United States in 1890. It was Horticultural Maturity Indices
introduced to California in 1904 at the Plant
Introduction Station in Chico, CA (Hendricks and The pistachio nut is a drupe; an exocarp and fleshy
Ferguson 1995). mesocarp surround the hard, but relatively thin,
shell, which encloses the edible seed. Ideally the
The pistachio tree is dioecious; thus orchard harvest is timed to the full accumulation of fat and
plantings must include the appropriate ratio sugar in the kernels. This is roughly coincident
of females and males (8:1 in older plantings, with the splitting of the shell. Shell split is not
but up to 25:1 in more recently established visible due to the fact that the fleshy mesocarp
orchards [Kallsen et al. 1995]). At present the masks the shell in developing nuts. However,
California industry is dominated by one male evidence of maturation can be seen in the color
cultivar (‘Peters’) and one female (‘Kerman’), change of the hull (exocarp), which is green when
although other cultivars are being tested. The the nut is not mature and then progresses through
reliance on single cultivars poses the potential ivory to rose with full maturation. Activity in
for catastrophic problems for the industry with the abscission zones between the nuts and the
pests and diseases, and efforts to evaluate existing rachis (assessed by a measurable decrease in
alternative germplasm and develop new cultivars “fruit removal force”) also indicates maturation.
are underway (Parfitt 1995a,b). There are also When fully mature, the nut with its shell will be
a limited number of rootstocks in use. While ejected from the hull when pressure is applied
the Verticillium-tolerant Pistacia integerrima with the thumb and forefinger at the hull’s distal
is currently the dominant rootstock in use, P. end (Ferguson et al. 1995). Nuts at full maturity,
atlantica × P. integerrima is increasingly being as judged by the preceding criteria, will have full
planted in California (Krueger and Ferguson accumulation of fat and simple sugars (Labavitch
1995). An important problem for pistachio et al. 1982).
growers is the strong tendency toward alternate
bearing. Harvest should not be delayed past full maturation
of the crop because this will increase losses to
navel orangeworm (Amyelois transitella Walker),
Quality Characteristics and Criteria birds, and fungi (particularly Aspergillus flavus),
as well as late season weather (rain and wind).
In-shell and shelled pistachios are marketed Furthermore, delayed harvest can lead to shell
extensively. An important aspect of quality of the staining because of breakdown of the phenolic-
772
compound-rich hull tissues. When harvested the described testing of a machine vision system that
nuts are split and moisture content is high (40 to might prove useful in sorting of nuts in processing
50%). Trees are shaken (by hand for young trees, streams to reduce the incidence of staining in the
mechanically for mature trees) and the nuts are marketed product. The simultaneous splitting of
caught on tarps or a catching frame and transferred shell and hull is generally caused by too tight an
to bins in order to eliminate problems caused by adherence of the hull to the shell. The absence of a
contact with the soil. tissue gap between the two pericarp-derived parts
of the nut makes it impossible for the shell to split
Delays between harvest and further processing without triggering hull split. This often leads to
should be minimized because they only shells with adhering hull material (Pearson et al.
exacerbate problems caused by hull breakdown or 1996), considered a defect.
contamination of hull tissues. Problems caused by
unavoidable delays in hulling and drying (below)
can be reduced by cold storage of bins at 0 ºC Optimum Storage Conditions
(32 ºF) and <70% RH without increasing shell
staining (Kader et al. 1980, Thompson 1997). At Once they have been dried (see above), nuts can
the processor, the bins of nuts are dumped, and be held at 20 ºC (68 ºF) and 65 to 70% RH for
debris is removed by passage over an air leg. up to a year (Ferguson et al. 1995). Pistachios
Hulls are removed, blanks are removed in a float are considerably less prone to rancidification
tank, and the in-shell nuts are dried to 5 to 7% (precipitated by oxidation of polyunsaturated fatty
moisture. Most large handlers now use a two-stage acids) than are almonds and, particularly, pecans
process: Nuts are dried in a column dryer to 12 to and walnuts. These commodities are also high in
13% moisture with forced hot air at 82 ºC (180 ºF) fat content, but walnut and pecan oils have a much
before the drying is completed more slowly (24 to higher content of polyunsaturated fatty acids than
48 h) with air heated to no more than 49 ºC (120 pistachio oil.
ºF) (Ferguson et al. 1995).
773
Respiration Rates Insect Problems
The low water content of properly stored, Several insects that are field pests of pistachios
dried pistachios makes them relatively inert are able to cause superficial damage (“epicarp
metabolically. Respiratory rates are very low. lesion”) to developing nuts. If insects are able
to probe deeply or introduce fungal pathogens,
these pests can cause damage to the kernels. The
Physiological Disorders navel orangeworm (Amyelois transitella Walker),
a primary field pest, is the major insect problem
Rancidification and shell staining have been after harvest. Methyl bromide fumigation has been
discussed in previous sections. used to control navel orangeworm in harvested
pistachios (Hartsell et al. 1986), but this fumigant
Developmental and physiological problems that is being phased out. Laboratory tests of navel
occur before full maturity can have particularly orangeworm survival during pistachio processing
important consequences for nut quality. Because indicate that very few of the insects survive nut-
nuts are only useful when they have split, failure drying (Johnson et al. 1996). Projections indicate
of hull split as nuts reach full maturity can that survival of navel orangeworms is insufficient
cause substantial yield losses. While splitting is to be a problem in stored nuts, particularly
maturation-dependant, it will be reduced by water because reinoculation of nuts due to insect
stress late in the growing season (mid-August reproduction within the dried, stored nuts is likely
through September) and failure to maintain to be virtually nonexistent (Johnson et al. 1996).
adequate boron (120 ppm [mg kg-1] leaf dry
weight). Early in postharvest drying, the split on
partially split nuts tends to widen (Freeman and Postharvest Pathology
Ferguson 1995).
Several fungi are capable of infecting growing
Blank nuts result when the embryo fails to pistachio nuts and causing damage to hulls and
develop. This can be caused by promotion of kernels. Infection is often facilitated by early
shell development from ovary tissues without splitting of hulls, which leads to infestations by
successful fertilization. Blank development also a number of hemipteran insects that feed on the
occurs later in the season and has been explained nuts and serve as nonspecific vectors for diseases.
as the result of the inability of the tree to provide Alternaria and Cladosporium species are also
sufficient assimilate to complete development of colonizers of early split nuts. Late season rains
its entire crop. Inadequate boron and water stress will promote activity of Botryosphaeria dothidea
are also indicated as causes of blank formation on pistachio hulls and kernels (Michailides et
(Freeman and Ferguson 1995). al. 1995). Because mold counts on nuts going
into storage can be high (Heperkan et al. 1994),
Pistachios are strongly alternate-bearing and it is important that proper storage conditions
failure of hull split and blank formation are (especially low RH and absence of standing water)
correlated with “on” and “off” crop years. Studies be maintained to avoid serious problems.
indicate that blanking tends to be much higher
in “off” years and nonsplit nuts are much more The greatest postharvest disease threats are from
common in “on” years. Of course, crop load is Aspergillus flavus and A. parasiticus. The danger
much higher in “on” years, and this has a large is particularly serious because these fungi can
effect on assimilate partitioning. produce aflatoxin. As with many disease problems
of pistachio, vectoring by insects attracted to
early split nuts (such as navel orangeworm) is
an important contributing factor. In Doster and
Michaelides’ (1994) study of Aspergillus molds
774
in California pistachios, early split nuts had Ferguson, L., A. Kader, and J.Thompson. 1995.
over 99% of the aflatoxin detected and navel- Harvesting, transporting, processing and grading.
orangeworm-infected nuts had substantially more In L. Ferguson, ed., Pistachio Production, pp.
infection by several Aspergillus species, as well 110-114. University of California, Pomology
as over 84% of the aflatoxin detected. The close Department, Center for Fruit and Nut Crop
association of contamination with early split nuts Research and Information, Davis, CA.
suggests that the potential aflatoxin problem can
be reduced by following procedures for reducing Freeman, M., and L. Ferguson. 1995. Factors
early splitting or sorting out nuts with shells affecting splitting and blanking. In L. Ferguson,
stained because of early splitting (discussed above ed., Pistachio Production, pp. 106-109. University
and Michaelides et al. 1995). In theory, nuts of California, Pomology Department, Center for
with aflatoxin contamination could be sorted out Fruit and Nut Crop Research and Information,
based on aflatoxin fluorescence; however, Steiner Davis, CA.
et al. (1992) concluded that this approach may
have limited value because of limited sensitivity Hartsell, P.L., H.D. Nelson, J.C. Tebbets, and P.V.
of detection and inconsistent presentation of Vail. 1986. Methyl bromide fumigation treatments
fluorescence in contaminated nuts. Further for pistachio nuts to decrease residues and
complicating the detection problem is the fact that control navel orangeworm (Amyelois transitella)
substantial contamination in a large sample of nuts (Lepidoptera: Pyralidae). J. Econ. Entomol.
may be due to the initial contamination and spread 79:1299-1302.
by only a few individual nuts.
Hendricks, L., and L. Ferguson. 1995. The
pistachio tree. In L. Ferguson, ed., Pistachio
Quarantine Issues Production, pp. 7-9. University of California,
Pomology Department, Center for Fruit and Nut
Insect infestation is a potentially important Crop Research and Information, Davis, CA.
problem, as are the fungal infections that often
accompany insect damage. Fumigation with Heperkan, D., N. Aran, and M. Ayfer. 1994.
methyl bromide or phosphine has been used for Mycoflora and aflatoxin contamination in shelled
disinfestation, but the former is being curtailed pistachio nuts. J. Sci. Food Agric. 66:273-278.
and insect resistance to phosphine has been
reported (Zettler et al. 1990). New fumigants are Johnson, J.A., R.F. Gill, K.A. Valero, and S.A.
being developed and tests of efficacy, including May. 1996. Survival of navel orangeworm
effects on flavor, are being performed (E.J. (Lepidoptera: Pyralidae) during pistachio
Mitcham, Univ. of California, Davis, 2002, processing. J. Econ. Entomol. 89:197-203.
personal communication). Thus far, the newer
fumigants are not registered for pistachio nuts. Kader, A.A., C.M. Heintz, J.M. Labavitch, and
H.L. Rae. 1982. Studies related to the description
and evaluation of pistachio nut quality. J. Amer.
References Soc. Hort. Sci. 107:812-816.
Doster, M.A., and T.J. Michailides. 1994. Kader, A.A., J.M. Labavitch, F.G. Mitchell,
Aspergillus molds and aflatoxins in pistachio nuts and N.F. Sommer. 1980. Quality and safety
in California. Phytopathology 84:583-590. of pistachio nuts as influenced by postharvest
handling procedures. In Pistachio Association
Doster, M.A., and T.J. Michailides. 1999. Annual Report, pp. 44-52.
Relationship between shell discoloration of
pistachio nuts and incidence of fungal decay and
insect infestation. Plant Dis. 83:259-264.
775
Kallsen, C., G.S. Sibbett, and C. Fanucchi. Pearson, T., D.C. Slaughter, and H.E. Studer.
1995. Planning and designing the orchard. In L. 1996. Hull adhesion characteristics of early-split
Ferguson, ed., Pistachio Production, pp. 36-40. and normal pistachio nuts. Appl. Eng. Agric.
University of California, Pomology Department, 12:219-221.
Center for Fruit and Nut Crop Research and
Information, Davis, CA. Steiner, W.E., K. Brunschweiler, E. Leimbacher,
and R. Schneider. 1992. Aflatoxins and
Krueger, B., and L. Ferguson. 1995. Pistachio fluorescence in brazil nuts and pistachio nuts. J.
rootstocks. In L. Ferguson, ed., Pistachio Agric. Food Chem. 40:2453-2457.
Production, pp. 41-42. Center for Fruit and Nut
Crop Research and Information, University of Thompson, J.F. 1997. Maintaining quality of
California, Pomology Department, Davis, CA. bulk-handled, unhulled pistachio nuts. Appl. Eng.
Agric. 13:65-70.
Labavitch, J.M., C.M. Heintz, H.L. Rae, and A.A.
Kader. 1982. Physiological and compositional Zettler, J.L., W.R. Halliday, and F.H. Arthur. 1990.
changes associated with maturation of ‘Kerman’ Phosphine resistance in insects infesting stored
pistachio nuts. J. Amer. Soc. Hort. Sci. 107:688- peanuts in the southeastern United States. J. Econ.
692. Entomol. 82:1508-1511.
776
Walnut kernel’s crispness. If water content drops too low,
however, damage to the kernel’s covering can
John M. Labavitch enhance O2 penetration and rancidity.
Labavitch is with the Department of Plant The skin covering the kernel contains chemicals
Sciences, University of California, Davis, CA. that protect fatty acids in the kernel from
becoming rancid. Light-colored kernels earn a
higher price because the light color indicates that
Scientific Name and Introduction the kernel still has a relatively long shelf-life. An
important criterion for evaluating new varieties
Persian (English) walnut (Juglans regia and a is yield of light-colored kernels (Hendricks et al.
few related species) is a member of the family 1998). Retention of light color is influenced by
Juglandaceae and is by far the most important the integrity of the seal between the two halves of
commercial walnut. The genus Juglans contains the shell, because the shell is an important barrier
three other groups (butternut, east asian species, to O2 entry. Shell strength and seal integrity are
and black walnut species) that produce edible also important in protecting kernels from insect
kernels. Persian walnuts evolved in central and damage and fungi infections that often follow
eastern Asia and were introduced into California insect damage. Two-thirds of the California
in the late 1700s. About 95% of U.S. production production is sold as shelled products (see Siebert
is in California. The most important cultivars 1998); therefore, additional characteristics of
are ‘Hartley,’ followed by ‘Chandler,’ ‘Serr,’ processing quality are ease of shelling and yield of
and ‘Vina.’ Recent cultivars such as ‘Tulare’ and intact kernel halves.
‘Howard’ will become more important.
Nuts are mechanically shaken from trees during Horticultural Maturity Indices
an extended harvest period from late August to
early November. The edible kernel is surrounded Kernels are considered mature when oil
by a husk that dehisces as the kernel nears accumulation is complete. This is generally
maturity. The husk, which should be well split and indicated by browning of the internal packing
relatively dry at harvest, is generally lost when tissue. However, harvest should not begin until the
the nuts fall to the ground. Harvested nuts (the husk is well split and separated from the shell. In
shell and kernel) may be 35% or more water. Nuts the hottest growing regions of California, kernels
are quickly swept from the orchard floor to avoid may be mature 3 weeks prior to husk “maturation”
damage to the fragile nut and are then dehydrated (dehiscence). Low temperatures and high RH, as
in forced-air dryers to 8% water content. The occurs in some growing regions and can occur at
temperature of the drying air is kept low, <43 night, advance dehiscence. Ethephon applications
°C (110 °F), to avoid damage leading to kernel are used to advance the harvest and make nut
rancidity. maturation more uniform throughout the tree/
orchard (Olson et al. 1998).
777
description of Federal quality standards can be Chilling Sensitivity
found at http://www.ams.usda.gov/AMSv1.0/ams.
fetchTemplateData.do?template=TemplateN&pag Walnuts are not sensitive to chilling. They may be
e=FreshNutandSpecCropStandards. stored at or below freezing.
Low water content and high fat content of the The most serious postharvest physiological
kernel make it relatively stable metabolically and disorder that affects walnut quality is oil rancidity.
able to tolerate low temperatures. The primary The problem appears to be caused by poor
objectives of storage are to maintain the low seed storage conditions: elevated temperature
water content attained after preliminary drying and RH and failure to use CA with reduced O2
(to suppress enzyme activity, retain texture, and concentration.
reduce microbial activity) and to limit exposure
to O2 (to minimize rancidity). The optimum
temperature range for storage is 0 to 10 °C (32 to Insect Problems
50 °F), with the lower temperature being better.
Within this temperature range, a 50 to 65% RH Insect damage can contribute to handling and
will maintain walnuts at 4% moisture (Beuchat quality problems. The major insect pest is the
1978). Lopez et al. (1998) modeled sorption codling moth (Cydia pomonella). While nuts
of walnut kernels and concluded that optimum attacked in mid season are likely to be rejected,
stability and texture were retained in a storage minor early codling moth damage can lead to
environment of 10 °C (50 °F) and 60% RH in air. more serious infestation with navel orangeworm
(Amyelosis transitella [Walker]), and this is more
difficult to detect if the nut is not shelled. The
Controlled Atmosphere Considerations walnut husk fly (Rhagoletis completa) is a serious
pest in mid to late season. Larval feeding damages
Shelf-life can be extended by storage in <1% O2. the husk tissues, leading to staining of the shell
O2 <0.5% (balance N2) or CO2 >80% in air can be and failure of the husk to split. This reduces the
effective in insect control. harvest yield and nuts that remain in the orchard
serve as a reservoir of insects to threaten the next
crop cycle (van Steenwyk and Barnett 1998).
In all cases, insect damage will tend to increase
problems with pathogen infection.
778
Postharvest Pathology changes in nut quality and stability could have
important effects on the potential for use of
Most infections with pathogens are initiated in walnuts in a variety of food products. However,
the orchard and transferred to the postharvest it is not certain that the work will be successful
environment. In-shell product is protected unless or that engineered products will be acceptable,
the shell has been broken or penetrated by insects. particularly since an important part of the crop is
The most serious pathogens are fungi such as destined for export. Whether or not new walnuts
Aspergillus flavus and A. parasiticus, which are introduced, it is certain that the most important
can produce aflatoxins that are both toxic and aspects of postharvest quality will be based on
carcinogenic. It is important that damaged kernels rapid harvest with minimal exposure to field heat,
be discarded prior to storage and that the low forced-air drying at relatively low temperature,
temperature and RH conditions discussed above and cold storage at an RH designed to maintain
be maintained in order to reduce the chance for low nut moisture in a reduced O2 atmosphere.
mold growth. Toxin-producing Penicillium sp.
have also been found on walnuts.
References
This presentation of quality maintenance Maté, J.I., M.E. Saltveit, and J.M. Krochta. 1996.
guidelines for walnuts has introduced some Peanut and walnut rancidity: effects of oxygen
discussion of changes of the fundamental concentration and RH. J. Food Sci. 61:465-
character of the nut that may be brought about by 468,472.
the use of genetic engineering. The anticipated
779
Olson, W.H., J.M. Labavitch, G.C. Martin, and
R.H. Beede. 1998. Maturation, harvesting and nut
quality. In D.E. Ramos, ed., Walnut Production
Manual, pp. 273-276. Pub. no. 3373, University
of California, Division of Agriculture and Natural
Resources, Davis. CA.
780