1 Department of Biotechnology, National Formosa University, Yunlin, Taiwan Keyword : Honey bee, Apis dorsata, anti-oxidant activity, anti-bacterial activity, Introduction Honey is the naturally sweet substance in the form of liquid produced by the honey bee Apis dorsata from the nectar of blossoms and plants giving nectar honeys, respectively in which fructose and glucose are the main contributors. Honey has highly variable sensorial and physicochemical characteristics due to climatic and environmental conditions and the diverse origins of the plants or depending on their floral source (Cimpoiu, Hosu, Miclaus, & Puscas, 2012). Indonesia is a tropical country rich with flora and fauna, harbors many different types of honey and the properties of the honeys produced by these bees have not been explored (LA Garibaldi, I Steffan‐Dewenter, C Kremen, 2011) Honey is a lot of substances, especially carbohydrates and water, minerals, proteins, free amino acids, enzymes, vitamins, organic acids, flavonoids, phenolic acids, and other phytochemicals (Terrab, Gonzalez, Diez, & Heredia, 2003). Honey is useful for the treatment of heart disease, cancer, cataracts, and some inflammatory diseases because can act as antioxidants and antimicrobials properties, as well as wound healing and anti-inflammatory activity (Martos et al., 2000). Anti-oxidant activity can reduce oxidation reactions in the food system and human health. Honey contains a lot of enzymatic and non-enzymatic antioxidants, including glucose oxidase, catalase, Ascorbic acid, a carotenoid derivative, organic acid, Maillard reaction products, amino acids, and proteins (Dimitrova, Gevrenova, & Anklam, 2007). The antibacterial properties of honey have been well known in folk medicine for many years. These healing powers are directly due to the honey's chemical composition, including the presence of hydrogen peroxide, as well as other non-peroxide factors. There are many nonperoxide constituents that form part of the antibacterial activity of honey and these include phenolic compounds, flavonoids and a number of other components Zainol et al. (2013), and Weston et al. (1999), Besides that, honey is a long-known anti-bacterial which inhibits the growth of 60 species of bacteria, including aerobes and anaerobes, and Grampositive and Gram-negative bacteria (Taormina, Niemira,&Beuchat, 2001). In this research, A. dorsata was selected for study since honey from this wild species is the most popular in local consumption and sales. For the purposes of the present research, the properties of honey, in terms of the anti-oxidant activity, anti- bacterial activity, and anti-inflammatory activity of Apis dorsata honey especially in Kalimantan.