APPENDIX

APPENDIX: -

GRAM STAINING: -

CRYSTAL VIOLET
GRAM IODINE SOLUTION
ETHYL ALCOHOL
SAFFRANINE

AMYLASE PRODUCTION (STARCH AGAR MEDIA)

STARCH 20 gm
PEPTONE 5 gm
BEEF EXTRACT 3 gm
AGAR 15 gm
DISTILLED WATER 1000 ml
(GRAM IODINE SOLUTION AS AN INDICATOR)

CELLULASE PRODUCTION TEST (CZAPEK MINERAL SALT AGAR MEDIA):-

SODIUM NITRATE 2 gm
POTASSIUM PHOSPHATE 1 gm
MAGNESIUM SULPHATE 0.5 gm
POTASSIUM CHLORIDE 0.5 gm
CARBOXYMETHYL CELLULOSE 5 gm
PEPTONE 2 gm
AGAR 20 gm
DISTILLED WATER 1000 ml
(C-TAB AS AN INDICATOR)
 APPENDIX

GELATINASE TEST (GELATIN AGAR MEDIA) :-

GELATIN 40 gm
TRYPTON 17 gm
PEPTONE 3 gm
SODIUM CHLORIDE 5 gm
DI POTASSIUM PHOSPHATE 2.5 gm
AGAR 15 gm
DISTILLED WATER 1000ml
(MERCURIC CHLORIDE AS AN INDICATOR)

CASEIN TEST (SKIM AGAR MEDIA):-

SKIM MILK POWDER 100 gm

PEPTONE 5 gm
AGAR 15 gm
DISTILLED WATER 1000ml

LITMUS MILK TEST (LITMUS MILK BROTH):-

LITMUS MILK 0.075 gm

SKIM MILK POWDER 100 gm
DISTILLED WATER 1000 ml

FERMENTATION OF CARBOHYDRATE (FERMENTATION BROTH):-

TRYPTICASE 10 gm
CARBOHYDRATE 5 gm
1. DEXTROSE
 APPENDIX

2. LACTOSE
3. SUCROSE
SODIUM CHLORIDE 15 gm
PHENOL RED 0.018 gm
DISTILLED WATER 1000 ml
IMViC TEST:-

INDOLE TEST (1% TRYPTON BROTH):-

TRYPTON 10 gm
SODIUM CHLORIDE 5 gm
CALCIUM CHLORIDE 1 gm
DISTILLED WATER 1000 ml

METHYL RED & VOGES PROSKAUER (MRVP BROTH):-

PEPTONE 7 gm

GLUCOSE 5 gm
POTASSIUM PHOSPHATE 5 gm

DISTILLED WATER 1000 ml

CITRATE UTILIZATION TEST (CITRATE AGAR MEDIA):-

MAGNESIUM SULPHATE 0.2 gm

SODIUM CHLORIDE 5 gm
AMMONIUM DIHYDROGEN PHOSPHATE 1 gm
DI POTASSIUM HYDROGEN PHOSPHATE 1 gm
SODIUM CITRATE 2 gm
BROMOTHYMOL BLUE 0.08 gm
AGAR 20 gm
DISTILLED WATER 1000 ml
 APPENDIX

UREASE TEST (UREA BROTH):-

PEPTONE 1 gm
SODIUM CHLORIDE 5 gm
POTASSIUM MONOHYDROGEN 2 gm
PHOSPHATE
AGAR 20 gm
DISTILLED WATER 1000 ml

HYDROGEN SULFIDE PRODUCTION TEST:-

PEPTONE 30 gm
BEEF EXTRACT 3 gm
FERRUS AMMONIUM SULPHATE 0.2 gm
SODIUM THIOSULPHATE 0.025 gm
AGAR 3 gm
DISTILLED WATER 1000 ml

CATALASE TEST (TRYPTICASE SOY BROTH):-

TRYPTICASE 15 gm
PHYTONE 5 gm
SODIUM CHLORIDE 5 gm
AGAR 15 gm
DISTILLED WATER 1000 ml

BIOFILM PRODUCTION:-

LURIA BERTANI MEDIA:-

 APPENDIX

TRYPTONE 10 gm
YEAST EXTRACT 5 gm
SODIUM CHLORIDE 10 gm
AGAR 15 gm
DISTILLED WATER 1000 ml

EFFECTS OF CARBON SOURCE (TRYPTONE YEAST EXTRACT GLUCOSE

BROTH) :-

TRYPTON 5 gm
YEAST EXTRACT 2.5 gm
CARBON SOURCE 1 gm
1. STARCH
2. DEXTROSE
3. SUCROSE
4. MANNITIOL
5. LACTOSE
DISTILLED WATER 1000 ml

SOIL TESTING METHOD:-

DETERMINATION OF pH OF SOIL:-

1. pH/R1 ( USING THIS REAGENT)HIMEDIA@ (SOIL TESTING KIT )

2. p H/R2 ( UNIVERSAL INDICATOR ) HIMEDIA@ ( SOIL TESTING KIT)

DETERMINATION OF AVAILABILTY OF PHOSPHATE OF SOIL:-

1. P/R1 HIMEDIA AVAILABLE PHOSPHATE (P) SOIL TESTING KIT

2. P/R2 HIMEDIA AVAILABLE PHOSPHATE (P) SOIL TESTING KIT
 APPENDIX

3. P/R3 HIMEDIA AVAILABLE PHOSPHATE (P) SOIL TESTING KIT

4. P/R4 HIMEDIA AVAILABLE PHOSPHATE (P) SOIL TESTING KIT