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○ Cdc25 initially inactive and the amount of wee1 is greater than the amount of M
CDK
○ Eventually, amount of M CDK becomes greater than Wee1 and M CDK becomes
active
○ Activation of M CDL activates the phosphatase and inactivates the kinase
resulting in rapid activation of the entire pool of M CDKs

○
● Turning key components on and off
○ How does APC get activated? The kinetochores send a signal until they sinse
tension. Once properly attached, they stop sending that signal. As a result, the
APC becomes active once all chromosomes are properly attached to the
spindles
● What does the APC do
○ E3 Ub ligase
○ Can have cdc20 or cdh1 bound to it. These target different proteins
○ Poly ub proteins that hold chromatids together so that sister chromatids separate
and anaphase happens
○ Inactivation of S and M CDKs

○
● Inactivation of APC by the G1 CDKs
○ G1 CDKs phosphorylate Cdh1 and then APC is inactive
● Discovery of the DNA Damage Checkpoint - lee Hartwell
○ WT = G1, S, G2, M
○ Radiated cells

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