13/9/2018

Table of Fluorochromes Texas Red 589 615 625 Sulfonyl chloride

Allophycocyanin
650 660 104 k
(APC)
(http://flowcyt.salk.edu/fluo.html) APC-Cy7
650;755 767 PharRed
conjugates
This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy.
Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission Alexa Fluor dyes [antibody conjugates] (Molecular Probes)
wavelengths often vary depending on the environment in which the probe finds itself. Be sure to also look up the
excitation and emission spectra for your dye of choice. Note that colors you might see with a capable browser are Alexa Fluor 350 343 442 410
only a very rough approximation!
Alexa Fluor 405 401 421 1028
Probe Ex (nm) Em (nm) MW Notes Alexa Fluor 430 434 540 702

Reactive and conjugated probes Alexa Fluor 488 499 519 643 QY 0.92
Alexa Fluor 500 503 525 700
Hydroxycoumarin 325 386 331 Succinimidyl ester
Alexa Fluor 514 517 542 714
Aminocoumarin 350 445 330 Succinimidyl ester
Alexa Fluor 532 530 555 724 QY 0.61
Methoxycoumarin 360 410 317 Succinimidyl ester
Alexa Fluor 546 561 572 1079 QY 0.79
Cascade Blue (375);401 423 596 Hydrazide
Alexa Fluor 555 553 568 1250 QY 0.1
Pacific Blue 403 455 406 Maleimide
Alexa Fluor 568 579 603 792 QY 0.69
Pacific Orange 403 551
Alexa Fluor 594 591 618 820 QY 0.66
Lucifer yellow 425 528
Alexa Fluor 610 610 629 1285
NBD 466 539 294 NBD-X
Alexa Fluor 633 632 648 1200
R-Phycoerythrin
480;565 578 240 k Alexa Fluor 647 652 668 1300 QY 0.33
(PE)
PE-Cy5 480;565;65 aka Cychrome, R670, Tri-Color, Alexa Fluor 660 663 691 1100
670
conjugates 0 Quantum Red Alexa Fluor 680 680 702 1150
PE-Cy7 480;565;74 Alexa Fluor 700 696 719 1400
767
conjugates 3
Alexa Fluor 750 752 776 1300
Red 613 480;565 613 PE-Texas Red
Alexa Fluor 790 782 804 1750
PerCP 490 675 Peridinin chlorphyll protein
TruRed 490,675 695 PerCP-Cy5.5 conjugate
Cy Dyes
FluorX 494 520 587 (GE Healthcare) Cy2 489 506 714 QY 0.12
Fluorescein 495 519 389 FITC; pH sensitive 570;(615
Cy3 (512);550 767 QY 0.15
)
BODIPY-FL 503 512
572;(620
TRITC 547 572 444 TRITC Cy3B 558 658 QY 0.67
)
X-Rhodamine 570 576 548 XRITC 594;(640
Cy3.5 581 1102 QY 0.15
Lissamine )
570 590
Rhodamine B Cy5 (625);650 670 792 QY 0.28

Cy5.5 675 694 1128 QY 0.23 TOTO-3, TO-

642 661
Cy7 743 767 818 QY 0.28 PRO-3
(Biostatus) (usable excitation down to
DyLight dyes (Pierce) DRAQ5 647 681,697 413
488)

DyLight 350 353 432 Cell function probes

DyLight 405 400 420
Indo-1 361/330 490/405 1010 AM ester. Low/High Ca++,
DyLight 488 493 518
Fluo-3 506 526 855 AM ester. pH > 6
DyLight 549 562 576
DCFH 505 535 529 2'7'Dichorodihydrofluorescein,
DyLight 594 593 618 oxidized form
DyLight 633 638 658 DHR 505 534 346 Dihydrorhodamine 123, oxidized form,
light catalyzes oxidation
DyLight 649 654 673
SNARF 548/579 587/635 pH 6/9
DyLight 680 692 712
DyLight 750 752 778 Fluorescent Proteins
DyLight 800 777 794 QY BR PS Source
Y66H 360 442
Nucleic acid probes Y66F 360 508
Hoechst 33342 343 483 616 AT-selective 0.1
EBFP 380 440 8
9 Addgene monomer
DAPI 345 455 AT-selective
0.5
Hoechst 33258 345 478 624 AT-selective EBFP2 383 448 6
18 Addgene monomer

SYTOX Blue 431 480 ~400 DNA 0.5

Azurite 383 447 5
14 monomer
Chromomycin A3 445 575 CG-selective
GFPuv 385 508
Mithramycin 445 575 0.6
T-Sapphire 399 511 0
26 25 weak dimer
YOYO-1 491 509 1271
0.6
Ethidium Bromide 493 620 394 TagBFP 402 457 26k 3
33 ++ Evrogen monomer
Acridine Orange 503 530/640 DNA/RNA 0.6
Cerulean 433 475 2
27 36 weak dimer
SYTOX Green 504 523 ~600 DNA
0.4
TOTO-1, TO- Vital stain, TOTO: Cyanine Dimer mCFP 433 475 0
13 64 monomer
509 533
PRO-1 TO-PRO: Cyanine Monomer 0.1
ECFP 434 477 5
3
Thiazole Orange 510 530
0.5
Propidium Iodide CyPet 435 477 1
18 59 weak dimer
536 617 668.4
(PI)
Y66W 436 485
DNA (543ex/712em), RNA
LDS 751 543;590 712;607 472 0.3
(590ex/607em) dKeima-Red 440 616 8 MBL dimer
1
7-AAD 546 647 7-aminoactinomycin D, CG-selective 0.2
mKeima-Red 440 620 4
3 MBL monomer
SYTOX Orange 547 570 ~500 DNA
TagCFP 458 480 0.5 29 Evrogen dimer

1
 13/9/2018

7 26.8 0.4
PhiYFP 525 537 0 52 ++ Evrogen weak dimer
0.7 k
AmCyan1 458 489 5
29 Clontech tetramer
26.8 0.3
0.8 PhiYFP-m 525 537 9 48 ++ Evrogen monomer
mTFP1 (Teal) 462 492 5
54 dimer k
0.6
S65A 471 504 ZsYellow1 529 539 5
13 Clontech tetramer
Midoriishi-Cyan 472 495 0.9 25 MBL dimer 0.7
mBanana 540 553 0
4 Clontech monomer
0.7
Wild Type GFP 396,475 508 26k 7
16
0.6
Kusabira-Orange 548 559 0
31 MBL monomer
S65C 479 507
0.6
0.5 mOrange 548 562 9
49 9 monomer
TurboGFP 482 502 26 k 3
37 Evrogen dimer
0.6
0.5 mOrange2 549 565 0
35 Clontech monomer
TagGFP 482 505 27k 9
34 ++ Evrogen monomer
0.6
TagGFP2 483 506 27k 0.6 34 ++ Evrogen monomer mKO 548 559 0
31 122 monomer
0.8 0.6
AcGFP1 484 510 27k 2
27 Clontech TurboRFP 553 574 26 k 7
62 Evrogen dimer

S65L 484 510 tdTomato 554 581

0.6
95 98 Clontech tandem dimer
9
0.6 0.6 Invitroge
Emerald 487 509 8
39
9 n weak dimer 0.4
DsRed-Express2 554 591 2
15 Clontech
S65T 488 511
0.4
0.6 TagRFP 555 584 27k 8
48 Evrogen monomer
EGFP 488 507 26k 0
34 174 Addgene weak dimer
3.
DsRed monomer 557 592 ~28k 0.1 5 16 Clontech monomer
tetramer
0.7
Azami-Green 492 505 4
41 MBL (monomeric ~110 0.5
available) DsRed2 ("RFP") 563 582 5
24 Clontech
k
0.9
ZsGreen1 493 505 105k 1
40 Clontech tetramer
mStrawberry 574 596
0.2
26 15 Clontech monomer
9
0.8 photoswitchabl 0.3
Dronpa-Green 503 518 5 81 MBL TurboFP602 574 602 26 k 26 Evrogen dimer
e 5
0.6 ~110 0.2
TagYFP 508 524 27k 2
47 Evrogen monomer AsRed2 576 592 tetramer
k 1 13 Clontech
0.6
EYFP 514 527 26k 1
51 60 weak dimer 0.2
mRFP1 584 607 ~30k 5
Tsien lab monomer
0.6
Topaz 514 527 0
57 monomer 0.2 8.
J-Red 584 610 0 8
13 dimer
0.5
Venus 515 528 7
53 15 weak dimer 0.2
mCherry 587 610 2
16 96 Clontech monomer
0.7
mCitrine 516 529 6
59 49 monomer 0.0 0.
HcRed1 588 618 ~52k 3 6
Clontech dimer
0.7
YPet 517 530 7
80 49 weak dimer 0.4
mKate2 588 633 26k 0
25 + Evrogen monomer
0.5
TurboYFP 525 538 26 k 3
56 Evrogen dimer 0.3
Katushka 588 635 26k 4
22 ++ Evrogen dimer

(TurboFP635)
mKate 0.3
588 635 0 15 Evrogen monomer
(TagFP635)
0.3
TurboFP635 588 635 26 k 4
22 Evrogen dimer
0.1 4.
mPlum 590 649 0 1
53 Clontech

monomer;
0.1 faster
mRaspberry 598 625 5 13 Clontech
photobleach
than mPlum
0.2 Tsien
mNeptune 600 650 0
13
Lab
monomer
0.2
E2-Crimson 611 646 3
29 Clontech

Other probes
Monochlorobiman
380 461 226 Glutathione probe
e
Calcein 496 517 623 pH > 5
HyPer 420/500 516 Hydrogen peroxide sensor