Aktifitas Antioksidan (penghambatan radikal bebas DPPH) Konjugat Kitosan

Konsentrasi Absorbansi Inhibisi (%)

Sampel
(ppm) A1 A2 A3 Rata2 Inhibisi 1 Inhibisi 2
Ao (tanpa sampel) 0 2.319 2.174 2.161 2.218
100 1.824 1.824 1.873 17.764 17.764
200 1.506 1.527 1.513 32.101 31.154
400 1.003 0.950 0.983 54.779 57.169
600 0.589 0.659 0.669 73.445 70.289
A kt ftas ant oksidan (%)

IC50
80 IC50 =
70 f(x) = 0.1181127763x + 4.4454722594
R² = 0.9794457019
60

50

40

30

20

10

0
0 100 200 300 400 500 600 700
Konsentrasi (ppm)
bebas DPPH) Konjugat Kitosan Asam Ferulat

Inhibisi (%) Rata2

SD Rata2 Inhibisi ± SD
Inhibisi 3 Inhibisi
0
15.555 17.027 1.28 17.027±1.28
31.785 31.680 0.48 31.680±0.48
55.681 55.876 1.21 55.876±1.21
69.838 71.190 1.97 71.190±1.97

Y = 0.118 X + 4.445
50 = 0.118 X + 4.445
X= 386.06 ppm
 Aktifitas Antioksidan (penghambatan radikal bebas DPPH) Konjugat Kito

Konsentrasi Absorbansi Inhibisi (%)

Sampel
(ppm) A1 A2 A3 Rata2 Inhibisi 1 Inhibisi 2
6 jam 0 0.4269 0.5012 0.4504 0.4595
1000 0.143 0.1359 0.1537 68.879 70.424
500 0.21 0.2199 0.2245 54.298 52.144
250 0.3507 0.3422 0.3546 23.678 25.528
125 0.4397 0.4101 0.4388 4.309 10.751
62.5 0.4881 0.4895 0.487 -6.224 -6.529

Aktftas antoksidan (%)

Konsentrasi (ppm) Rata2 Inhibisi

0 0
1000 68.6180631121 Rata2 Inhibisi vs ppm
500 52.5281102648 80
70 f(x) = 0.0769372707x - 0.6054199699
250 24.011606819 R² = 0.9088626443
125 6.5215814291 60

62.5 -6.2459194777 50
40
30
20
10
0
-10 0 200 400 Konsentrasi
600(ppm) 800 1000 120
bebas DPPH) Konjugat Kitosan Asam Ferulat

Inhibisi (%) Rata2

SD Rata2 Inhibisi ± SD
Inhibisi 3 Inhibisi
0
66.551 68.618 1.95 68,618±1,95
51.143 52.528 1.61 52,528±1,61
22.829 24.012 1.38 24,012±1,38
4.505 6.522 3.66 6,552±3,663
-5.985 -6.246 0.27 (-)6,246±0,27

IC50 y = 0,0769x-0,6054
IC50 50 = 0,0769x-0,6054
s ppm IC50 x = 65,807 ppm

m) 800 1000 1200

 Aktifitas Antioksidan (penghambatan radikal bebas DPPH) Konjugat Kitosan Asam

Konsentrasi Absorbansi Inhibisi (%)

Sampel
(ppm) A1 A2 A3 Rata2 Inhibisi 1 Inhibisi 2 Inhibisi 3
12 jam 0 0.4269 0.5012 0.4504 0.4595
1000 0.1234 0.1147 0.1242 73.145 75.038 72.971
500 0.1728 0.1826 0.1778 62.394 60.261 61.306
250 0.2465 0.2782 0.2831 46.355 39.456 38.390
125 0.3642 0.3757 0.4002 20.740 18.237 12.905
62.5 0.4483
Akt ftas ant oksidan (%) 0.4566 0.4443 2.437 0.631 3.308

Konsentrasi Rata2 Inhibisi

(ppm)
0 0
1000 73.717809213 Rata2 Inhibisi vs ppm
500 61.320275662 80
f(x) = 0.0761301421x + 8.0592776828
250 41.400072543 70 R² = 0.8496175616
125 17.294160319 60
62.5 2.1254987305 50
40
30
20
10
0
0 200 400 600 800 1000 1200
konsentrasi (ppm)
H) Konjugat Kitosan Asam Ferulat

Rata2
SD Rata2 Inhibisi ± SD
Inhibisi
0
73.718 1.15 73,718±1,15
61.320 1.07 61,32±1,07
41.400 4.32 41,400±4,32
17.294 4.00 17,294±4,00
2.125 1.37 2,125±1,37

IC50 y = 0,0761x + 8,0593

IC50 50 = 0,0761x + 8,0593
IC50 x =551,126 ppm

1000 1200
 Aktifitas Antioksidan (penghambatan radikal bebas DPPH) Konj

Konsentrasi Absorbansi Inhibisi (%)

Sampel
(ppm) A1 A2 A3 Rata2 Inhibisi 1
18 jam 0 0.4269 0.5012 0.4504 0.4595
1000 0.246 0.2328 0.2181 46.464
500 0.3415 0.3381 0.3472 25.680
250 0.4469 0.4551 0.4375 2.742
125 0.5127 0.5124 0.5006 -11.578
62.5 0.5539 0.5709 0.565 -20.544
Aktftas antoksidan (%)

Konsentrasi Rata2 Inhibisi

(ppm)
0 0
1000 49.445048966 konsentrasi vs rata2 inhibisi
60
500 25.513239028
50
250 2.8291621328 f(x) = 0.0648154726x - 13.5088864708
40 R² = 0.8734979898
125 -10.67827349
30
62.5 -22.58251723
20
10
0
-10 0 200 400 600 800 1000 1200

-20
konsentrasi (ppm)
-30
hambatan radikal bebas DPPH) Konjugat Kitosan Asam Ferulat

Inhibisi (%) Rata2

SD Rata2 Inhibisi ± SD
Inhibisi 2 Inhibisi 3 Inhibisi
0
49.336 52.535 49.445 3.04 49,445±3,04
26.420 24.440 25.513 1.00 25,513±1,00
0.958 4.788 2.829 1.92 4,778±1,92
-11.513 -8.945 -10.678 1.50 (-)8,945±1,50
-24.244 -22.960 -22.583 1.88 (-)22,583±1,87

IC50 y = 0,0648x - 13,509

IC50 50 = 0,0648x - 13,509
inhibisi IC50 x = 980,077 ppm

800 1000 1200

m)
 Aktifitas Antioksidan (penghambatan radikal bebas DPPH) Konjugat Kitos

Konsentrasi Absorbansi Inhibisi (%)

Sampel
(ppm) A1 A2 A3 Rata2 Inhibisi 1 Inhibisi 2
6 jam 0 0.4269 0.5012 0.4504 0.4595
1000 0.1234 0.1329 0.1356 73.145 71.077
500 0.1281 0.2058 0.1882 72.122 55.212
250 0.2617 0.3094 0.2893 43.047 32.666
125 0.362 0.4163 0.4285 21.219 9.402
62.5 0.4934
aktvitas antoksidan % 0.4926 0.5293 -7.378 -7.203

Konsentrasi Rata2 Inhibisi

(ppm)
0 0
1000 71.570547697
konsentrasi vs rata2 inhibisi
80
500 62.125498731 70
f(x) = 0.0804655297x + 2.9850251308
R² = 0.8148203152
250 37.584330794 60
125 12.455567646 50
62.5 -9.92383025 40
30
20
10
0
-10 0 200 400 600 800 1000 1200
konsentrasi (ppm)
-20
ebas DPPH) Konjugat Kitosan Asam Ferulat

ibisi (%) Rata2 Inhibisi ±

Rata2 Inhibisi SD
Inhibisi 3 SD
0
70.490 71.571 1.39 71,51±1,39
59.042 62.125 8.87 62,125±8,87
37.040 37.584 5.21 37,584±5,21
6.746 12.456 7.70 12,456±7,70
-15.190 -9.924 4.56 (-)9,942±4,56

IC50 y = 0,0805x + 2,985

IC50 50 = 0,0805x + 2,985
IC50 x = 584,037 ppm

1000 1200