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N0 [Amilum]Abs StandarAbs Sampel Abs [Bereaksi] C

1 0.01 0.07 - - -
2 0.02 0.08 - - -
3 0.1 0.1 0.2405 0.1405 -0.10095012
4 0.5 0.132 0.2055 0.0735 -0.49881235
5 1 0.392 0.2085 0.1835 0.1543943
6 2 0.866 0.3115 0.5545 2.35748219
7 5 0.981 0.2735 0.7075 3.26603325
8 10 1.776 0.3395 1.4365 7.59501188

Kurva Kalibrasi Standar


2

f(x) = 0.1683756756x + 0.1575201455


1.5 R = 0.9321709757

1
Absorbansi

0.5

0
0 2 4 6 8 10

Konsentrasi Amilum

Kurva Michaelis-Menten (Amilase 20mg/ml)


2

1.5
f(x) = 0.2x - 1.81298660734736E-016
1R =1
V (mg/ml menit)
0.5

0
-1 0 1 2 3 4 5 6 7 8
-0.5

Konsentrasi Bereaksi (mg/ml)


V 1/C 1/V sampel Abs 1 Abs 2
- - - 0.1 0.237 0.244
- - - 0.5 0.206 0.205
-0.02019 -9.905882 -49.52941 1 0.171 0.246
-0.099762 -2.004762 -10.02381 2 0.337 0.286
0.0308789 6.4769231 32.384615 5 0.294 0.253
0.4714964 0.4241814 2.1209068 10 0.362 0.317
0.6532067 0.3061818 1.5309091
1.5190024 0.1316654 0.6583268

Kurva Lineweaver-Burk (Amilase 20mg/ml)


40
30
f(x) = 5x - 2.17558392881683E-015
20
R = 1 10
0
1/V -12 -10 -8 -6 -4 -2 -10 0 2
-20
-30
-40
-50
8 10 12 -60

1/Konsentrasi Bereaksi

Y = mX + C
e 20mg/ml)
C = 5 x 10 Vmax = 0E+015
M=5 KM = 1.0000E+016

734736E-016

5 6 7 8 9

(mg/ml)
D Abs
0.2405
0.2055
0.2085
0.3115
0.2735
0.3395

Amilase 20mg/ml)
40
30
3E-015
20
10
0
-10 0 2 4 6 8
-20
-30
-40
-50
-60

Bereaksi

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