S(mM) V0(mMs-1)

0.25 0.75
0.5 1.2 Chart Title
1 1.71 3
2 2.18
4 2.53 2.5
7 2.6
2
10 2.63
13 2.65 1.5

0.5

0
0 2 4 6 8 10 12 14
10 12 14
Concentración(milimol) Absorvancia
0 -0.005297
0.4 0.138303
0.8 0.281903
1.2 0.425503
1.6 0.569103 Chart Title
2 0.712703 0.8
0.7
0.6 f(x) = 0.109239153846154 x
R² = 0.976985068790805

Absorbancia (D.O)
0.5
0.4
0.3
0.2
0.1
0
0 0.4 0.8
-0.1
Concentración

sustrato (mM) D.O [Glucosa] mM Vmax(mM/min 1/[S] 1/Vmax

0.0085 -0.0053 -0.0287308229 -0.00287308229 117.647059 -348.058252
0.0489 0.0665 0.17154811715 0.017154811715 20.4498978 58.2926829
0.085 0.112452 0.29972663877 0.029972663877 11.7647059 33.3637345
0.171 0.195022 0.5300474198 0.05300474198 5.84795322 18.8662365
0.18 0.198971 0.54106276151 0.054106276151 5.55555556 18.4821442
 Chart Title

f(x) = 0.109239153846154 x
R² = 0.976985068790805

0 0.4 0.8 1.2 1.6 2

Concentración mM

Chart Title
por tener un valor negativo no se toma
70

60
f(x) = 2.67652560384446 x + 3.06495089195743
50 R² = 0.998110255724117
40
1/Vmax

30

20

10

0
4 6 8 10 12 14 16 18 20 22
1/[S]
Glucosa (mM) D.O
0 0 curva de calibracion
0.1 0.024 1.2
0.2 0.115
1 f(x) = 1.14727272727273 x − 0.0703636363636364
0.3 0.259 R² = 0.980250884607975
0.4 0.299 0.8
0.5 0.536
0.6

D.O
0.6 0.617
0.7 0.79 0.4
0.8 0.908
0.2
0.9 0.983
1 1.005 0
0 0.2 0.4 0.6 0.8 1 1.2
Glucosa [mM]

250
Sustrato mM D.O [Glucosa]mM Vmax(mM/min) 1/[S] 1/Vmax
0.006 -0.0053 0.0567419158 0.00378279439 166.666667 264.354839 200
f(x) = 10.0374471559
0.07 0.01624 0.07551642988 0.00503442866 14.2857143 198.632271
150
0.9 0.14189 0.18503442866 0.01233562858 1.11111111 81.0659946

1/Vmax
1.69 0.22805 0.26013248496 0.01734216566 0.59171598 57.6629251
100
2 0.23523 0.26639065632 0.01775937709 0.5 56.3082813
2.2 0.23882 0.269519742 0.0179679828 0.45454545 55.6545502 50

0
0 2 4

0.006 0.00378279
0.07 0.00503443
0.9 0.01233563
1.69 0.017342166.
2 0.01775938
2.2 0.01796798 Chart Title
0.02
0.018
0.016
0.014
0.012
0.01
Vmax

0.008
0.006
0.004
0.002
0
0 0.5 1 1.5 2 2.5
Sustrato (mM)
0.006
0.004
0.002
0
0 0.5 1 1.5 2 2.5
Sustrato (mM)
8 1 1.2
15min de incubacion T ambiete

Chart Title
250

200
f(x) = 10.0374471559482 x + 55.8517367866175

150
1/Vmax

100

50

0
0 2 4 6 8 10 12 14 16
1/[S]

2 2.5
2 2.5
Sustrato (mM) Vo(mM/S) 1/[S] 1/Vmax
0.25 0.75 4 1.33333333
0.5 1.2 2 0.83333333
1 1.71 1 0.58479532
2 2.18 0.5 0.4587156
4 2.53 0.25 0.39525692 Chart Title
1.4
f(x) = 0.249950953927358 x + 0.333662921738678
1.2

0.8

1/Vmax
0.6

0.4

0.2

0
0 0.5 1 1.5 2 2.5 3
1/[S]
 Chart Title

27358 x + 0.333662921738678

1.5 2 2.5 3 3.5 4 4.5

1/[S]
Sustrato (mMV(mu/s 1/[S]
0.0085 0.00038
0.0489 0.0017
0.085 0.003
0.17 0.0048
0.257 0.006