V (mM min-1)

S (mM) Sin inhibidor Inhibidor A

1.25 1.72 0.98
1.67 2.04 1.17
2.50 2.63 1.47
5.00 3.33 1.96
10.00 4.17 2.38

Sin inhibidor Vmax= 4.5 Km= 2.25

Con Inhibidor Vmax=2.5 Km=1.25

[aa] (uM) 12,5

V (u.a.) - Hidroxiácido 13,3
+ Hidroxiácido

[aa] (uM) - Hidroxiácido + Hidroxiácido

12.50 13.30
16.67 17.20 4.60
25.00 22.20 6.40
50.00 33.30 11.80
100.00 40.00 20.00
[S] mM 2.0 3.0
g P/h (sin inhib.) 139 179
g P/h (con inhib. 6 mM) 88 121

[S] mM g P/h (sin inhib.) g P/h (con inhib. 6 mM)

2.0 139 88
3.0 179 121
4.0 213 149
10.0 313 257
15.0 370 313
 Gráfico de Michaelis-Menten
4.5
4 f(x) = 1.17218775549786 ln(x) + 1.47352360864673
3.5
3
2.5
2 f(x) = 0.680832256249776 ln(x) + 0.834331614040446
1.5
1
0.5
0
0.00 2.00 4.00 6.00 8.00 10.00 12.00

V (mM min-1) Logarithmic (V (mM min-1))

Column D Logarithmic (Column D)

16,67 25 50 100
17,2 22,2 33,3 40
4,6 6,4 11,8 20,0

Chart Title
45.00
40.00
35.00
30.00
25.00
20.00
15.00
10.00
5.00
0.00
0.00 20.00 40.00 60.00 80.00 100.00 120.00

- Hidroxiácido + Hidroxiácido
 4.0 1.0 15.0
213 313 370
149 257 313

Chart Title
400

350

300

250

200

150

100

50

0
0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0

g P/h (sin inhib.) g P/h (con inhib. 6 mM)

 1.20
V (mM min-1)
S (mM) Sin inhibidor Inhibidor A 1.00
f(x) = 0.85816296686662
0.80 0.58 1.02
0.60 0.49 0.85 0.80
0.40 0.38 0.68
0.20 0.30 0.51 0.60
f(x) = 0.48431276187116
0.10 0.24 0.42
0.40
Con inhibidor Vmax Km
2.97 0.8582x=-0.3369 0.20
x= -0.3925
2.54777070063694 0.00
0.00 0.10 0.20
Vmax Km
Sin inhibidor 5.1255766273706 0.4843x=-0.1951 Sin inhibidor

x= -0.4028
2.48262164846077 Conclusión
No competitiva ya que el el Vmax es di

[aa] (uM) - Hidroxiácido + Hidroxiácido

0.08 0.08
0.25
0.06 0.06 0.22 f(x) = 3.36742522751315
0.04 0.05 0.16 0.20
0.02 0.03 0.08
0.01 0.03 0.05 0.15

Sin inhibidor Vmax Km 0.10

60.240963855422 0.7174x= -0.0166 f(x) = 0.717401227535364
x= -0.0231 0.05

43.2900432900433
0.00
120.00
Vmax Km 0.00 0.01 0.02
Con inhibidor 56.497175141243 3.3674x= -0.0177
x= -0.0051
196.078431372549
 Conclusión
Es competitiva, ya que aunque Vmax no

[S] mM g P/h (sin inhib.)g P/h (con inhib. 6 mM)

0.5 0 0
0.3 0 0 0
0.3 0 0
0.1 0 0
0.1 0 0
Vmax Km
Sin inhibidor 476.19047619048 0.0103x= -0.0021 0 f(x) = 0.0187982700375
x= -0.2038
4.90677134445535 f(x) = 0.0102694123830

Con inhibidor Vmax Km

16.0 500 0.0188x= -0.002 0
0.0 0.1
x= -0.1063
9.40733772342427 g
g

Conclusión
Es acompetitiva, ya que Vmax varía y
 Linealizado

f(x) = 0.858162966866622 x + 0.336927757065883

f(x) = 0.48431276187116 x + 0.195090246656494

0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90

Sin inhibidor Linear (Sin inhibidor) Inhibidor A Linear (Inhibidor A)

va ya que el el Vmax es diferente pero el Km permanece igual

Linealizado
f(x) = 3.36742522751315 x + 0.0176655471263768

f(x) = 0.717401227535364 x + 0.0165513858388987

0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 0.09

- Hidroxiácido Linear (- Hidroxiácido)

+ Hidroxiácido Linear (+ Hidroxiácido)
a, ya que aunque Vmax no es exactamente igual es parecido, mientras que el Km aumenta

Linealizado

f(x) = 0.018798270037588 x + 0.00198552195702264

f(x) = 0.0102694123830066 x + 0.00210729957333938

0.1 0.2 0.3 0.4 0.5 0.6

g P/h (sin inhib.) Linear (g P/h (sin inhib.))

g P/h (con inhib. 6 mM) Linear (g P/h (con inhib. 6 mM))

tiva, ya que Vmax varía y también lo hace el Km