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  • Succinate Deshidrogenasa

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    Alejandra Hoyos Salcedo
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    This document contains data and calculations related to the rate of fumarate formation from succinate at different temperatures and substrate concentrations. It shows that: 1) The rate of fumarate formation increases with increasing succinate concentration up to 25mM and is higher at 35°C than 25°C at all concentrations tested. 2) Lineweaver-Burk plots were constructed which yielded apparent Km and Vmax values that decreased with increasing temperature, indicating the reaction is faster at higher temperatures. 3) Calculations of activation energy (Ea) and entropy (ΔH°) from the temperature dependence of Km and Vmax suggest the reaction is enthalpically driven.

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    Succinate Deshidrogenasa.xlsx

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    This document contains data and calculations related to the rate of fumarate formation from succinate at different temperatures and substrate concentrations. It shows that: 1) The rate of fumarate formation increases with increasing succinate concentration up to 25mM and is higher at 35°C than 25°C at all concentrations tested. 2) Lineweaver-Burk plots were constructed which yielded apparent Km and Vmax values that decreased with increasing temperature, indicating the reaction is faster at higher temperatures. 3) Calculations of activation energy (Ea) and entropy (ΔH°) from the temperature dependence of Km and Vmax suggest the reaction is enthalpically driven.

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    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
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    4 views5 pages

    Succinate Deshidrogenasa

    Uploaded by

    Alejandra Hoyos Salcedo
    This document contains data and calculations related to the rate of fumarate formation from succinate at different temperatures and substrate concentrations. It shows that: 1) The rate of fumarate formation increases with increasing succinate concentration up to 25mM and is higher at 35°C than 25°C at all concentrations tested. 2) Lineweaver-Burk plots were constructed which yielded apparent Km and Vmax values that decreased with increasing temperature, indicating the reaction is faster at higher temperatures. 3) Calculations of activation energy (Ea) and entropy (ΔH°) from the temperature dependence of Km and Vmax suggest the reaction is enthalpically driven.

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    © All Rights Reserved
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    of 5

    Rate of fumarate formation

    [Succinate] V 35°C 10^2 V 25°C 10^2


    mM (mmol/min*mg proteina) (mmol/min*mg proteina)

    2.5 0.075 0.037


    5 0.092 0.048
    10 0.104 0.057
    15 0.109 0.061
    20 0.112 0.063
    25 0.113 0.064

    35
    3.00E+01
    2.00E+01 f(
    R

    1/V
    1.00E+01 f(
    R
    0.00E+00
    0 0.05
    1/v 35°C 1/V 25°
    1/[Succinate]
    mM (mmol/min*mg (mmol/min*mg
    proteina) proteina)
    0.4 1.33E+01 2.70E+01
    0.2 1.09E+01 2.08E+01 T°K Km / Vmax
    0.1 9.62E+00 1.75E+01 308.15 12.513
    0.06666666667 9.17E+00 1.64E+01 298.15 31.874
    0.05 8.93E+00 1.59E+01
    0.04 8.85E+00 1.56E+01
    1/T LN (Vmax app)
    0.00324517 -2.12129100817
    1/S VS 1/V
    0.00335402 -2.66249479944
    35 Linear (35) 25 Linear (25)
    3.00E+01 1/T VS LN (Vmax app)
    2.00E+01 f(x) = 31.8742386051755 x + 14.331680127836
    R² = 0.999726786913895 0
    0.0032 0.00325 0.0033 0.00335 0.0
    1/V

    1.00E+01 f(x) = 12.5126159793941 x + 8.34193050351041 -0.5


    R² = 0.999808659106192 -1
    0.00E+00
    0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 -1.5
    1/S -2
    -2.5 f(x) = − 4972.3056379892 x + 14.01470002862
    R² = 1
    -3

    1/T VS LN(Km app)


    1
    0.8
    f(x) = 3618.20657830591 x − 11.3362284967
    0.6 R² = 1
    0.4
    0.2
    0
    0.0032 0.00325 0.0033 0.00335 0.
    1/Vmax Vmax app Km app
    8.3419 0.11987677 1.50001798
    14.332 0.06977393 2.22397432

    LN (Km app)
    0.40547709571
    0.79929583099

    LN (Vmax app) Ea/R LN Vmax Ap,0 Ea Vmax Ap,0


    -4972.3 14.015 -9845.154 1220779.3204
    0.0033 0.00335 0.0034

    3056379892 x + 14.0147000286277

    𝑣= (1220779,32∗ 𝑒^((−(−9845,154)/(1,98 ∗ 𝑇)
    S LN(Km app) DH°/R LN Km Ap,0 DH° Km Ap,0
    3618.2 -11.336 7164.036 1.193542E-05

    20657830591 x − 11.3362284967125

    0.0033 0.00335 0.0034


    5,154)/(1,98 ∗ 𝑇)) )∗𝑆)/(1,19354∗ 〖 10 〗 ^(−05)∗ 𝑒^((7164,036) )+
    ^((7164,036) )+𝑆)

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