Rate of CO2 Evolution, v (mmoles/l-h) Lineweaver-Burk Plot Data

Glyox, S (mM) I=0 (v) I=1.26mM (I1) I=1.95mM (I2) 1/S 1/v 1/I1 1/I2
0.25 1.02 0.73 0.56 4 0.980392 1.369863 1.785714
0.33 1.39 0.87 0.75 3.030303 0.719424 1.149425 1.333333
0.4 1.67 1.09 0.85 2.5 0.598802 0.917431 1.176471
0.5 1.89 1.3 1 2 0.529101 0.769231 1
0.6 2.08 1.41 1.28 1.666667 0.480769 0.70922 0.78125
0.75 2.44 1.82 1.39 1.333333 0.409836 0.549451 0.719424
1 2.5 2.17 1.82 1 0.4 0.460829 0.549451
Vm, mmol/l-h 6.3371356 6.281407 6.5189048
Km, mmol/l 1.2294043 1.945979 2.6323337

Eadie-Hoffstee Plot Data Hanes-Wolf Plot Data

I=0 (v) Glyox, S (mM) v/S Glyox, S (mM) I=0 (v) S/v
1.02 0.25 -4.08 0.25 1.02 0.245098
1.39 0.33 -4.2121212121 0.33 1.39 0.23741
1.67 0.4 -4.175 0.4 1.67 0.239521
1.89 0.5 -3.78 0.5 1.89 0.26455
2.08 0.6 -3.4666666667 0.6 2.08 0.288462
2.44 0.75 -3.2533333333 0.75 2.44 0.307377
2.5 1 -2.5 1 2.5 0.4
Vm, mmol/l-h 4.5821 Vm, mmol/l-h 4.7125355
Km, mmol/l 0.7494 Km, mmol/l 0.78746476

1/v vs 1/S (Lineweaver-Burk Plot) Lineweaver-Burk Plot data for v, I1 and I2

1.2 2
1.8
1 f(x) = 0.403832929951864 x + 0.153427920199648
1.6
R² = 0.993685653006745
f(x) = 0.194044761725607 x + 0.15782155527227
0.8 R² = 0.970633012452565 1.4 1/
f(x) = 0.309798793698001 x + 0.159168717012588 v
1.2 R² = 0.991243455280152 vs
v, I1, I2

0.6
1/v

1 1/
f(x) = 0.194044761725607 x + 0.15782155527227 S
0.8
0.4 R² = 0.970633012452565
0.6
0.2 0.4
0.2
0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 0
0.5 1 1.5 2 2.5 3 3.5 4 4.5
1/S 1/S

v/S vs v (Eadie-Hoffstee Plot) S vs S/v (Hanes-Wolf Plot)

3 0.45

0.4
f(x) = 0.749389607261204 x + 4.58211370903214 2.5
R² = 0.736698095300828 0.35 f(x) = 0.212192406247465 x + 0.167103000786615
R² = 0.916608718083849
2 0.3

0.25
S/v

1.5 0.2
v

0.15
1
0.1

0.5 0.05

0
0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1
0
-4.4 -4.2 -4 -3.8 -3.6 -3.4 -3.2 -3 -2.8 -2.6 -2.4
S
v/S