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1/s v

Concentracion de Ls Vel. Inicial 1/S 1/V 0.30


isositrato milimol/min-1
micromol/decimetro-1 0.25
f(x) = 0.00220563131
0.018 4.08 55.56 0.25 R² = 0.999868418235
0.20
0.024 4.64 41.67 0.22
0.03 5.1 33.33 0.20 0.15

1/V
0.04 5.62 25 0.18
0.1 6.9 10 0.14 0.10

0.05

Utilizando la ec de Lineaweaver Burk tenemos que: 0.00


0.00 10.00 20.00
1/𝑉=𝐾𝑚/𝑉𝑚𝑎𝑥∗1/𝑆+1/𝑉𝑚𝑎𝑥

1/𝑉=2.2056 1/𝑆+0.1229

1/Vmax= 0.1229 km/Vmax= 0.0022


Vmax= 1/0.1229 km/8.137= 0.0022
Vmax= 8.14 mM/min km= (0.0022)(8.137)
Km= 0.018 mM
1/s vs 1/v
0

5
f(x) = 0.002205631311422 x + 0.122880534829349
R² = 0.999868418235791
0

0
0.00 10.00 20.00 30.00 40.00 50.00 60.00
1/S

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