V= μmol de AY/min*gEnz

T1= 50°C T2= 90°C

[AYI](M) [AC](0mM) [AC](15mM) [AC](30mM) [AYI](M) [AC](0mM)
(AU min^-1) (AU min^-1) (AU min^-1) (AU min^-1)
0.432 0.293 0.13 0.094 0.432 0.425
1.44 0.705 0.374 0.274 1.44 1.001
14.256 0.842 0.66 0.613 14.256 1.162

Inverso de T1=50°C Inverso de T1=90°C

1/[s] 1/v1 1/v2 1/v3 1/[s]
2.31481481 3.41296928 7.69230769230769 10.6382979 2.31481481
0.69444444 1.41843972 2.67379679144385 3.64963504 0.69444444
0.0701459 1.18764846 1.51515151515152 1.63132137 0.0701459

inverso t1=50°c
12 7

6 f(x) = 2.49044295
10 f(x) = 4.07280748691742 x + 1.12580995903107 R² = 0.996788897
R² = 0.996682593020446 5
1/v1 f(x) = 1.79885833
8 4 R² = 0.989788122

Axis Title
f(x) = 2.82110444703478 x + 1.06464413335987 Linear (1/v1)
Axis Title

6 R² = 0.990921299844072 1/v2 3
Linear (1/v2)
2 f(x) = 0.69905899
4 1/v3
R² = 0.964789865
Linear (1/v3) 1
f(x) = 1.03938227578446 x + 0.939459436511986
2 R² = 0.9685281650327
0
0 0.5
0
0 0.5 1 1.5 2 2.5
Axis Title

A B
0 1.0394 0.9395
15 2.8211 1.0646
30 4.0728 1.1258

[AC] Vmax(ap) Km(ap)

0 1.06439596 1.10633315593401
15 0.93931993 2.64991546120609
30 0.88825724 3.61769408420679

Inhibicion mixta tipo I

 [AC] 1/Vmax (ap) Km(ap)/Vmax (ap)
0 0.9395 1.0394
15 1.0646 2.8211
30 1.1258 4.0728

1/vmax(ap) y km(ap)/vmax(ap)
4.5
4 f(x) = 0.101113333333333 x + 1.12773333333333
3.5 R² = 0.989926645134164
3
Column C
Axis Title

2.5
Linear (Column C)
2 Column D
1.5 Linear (Column D)
1 f(x) = 0.00621 x + 0.95015
0.5 R² = 0.962264574662395
0
0 5 10 15 20 25 30 35
Axis Title

50°C
V 1/vmax(ap) km(ap)/vmax(1/vmax* KINC 1/VMAX km/vmax km/vmax*KI
0 0.9395 1.0394 0.0062 0.9502 1.1277 0.1011
15 1.0646 2.8211
30 1.1258 4.0728

T°C T°K
50 323.15
90 363.15

Ln Km Ln Vmax
0.18 0.4
0.16 f(x) = 209.953122457176 x − 0.478444994608733 0.35 f(x) = − 920.110438230988 x + 2.
0.14 R² = 1 R² = 1
0.3
0.12
0.25
Axis Title

0.1
Axis Title

Column H 0.2
0.08 Linear (Column H)
0.06 0.15
0.04 0.1
0.02 0.05
0
0
0.0027 0.0028 0.0029 0.003 0.0031 0.0032
0.0027 0.0028 0.0029 0.003 0.0031 0.0
Axis Title
Axis Title
 0.05
0
0
0.0027 0.0028 0.0029 0.003 0.0031 0.0032
0.0027 0.0028 0.0029 0.003 0.0031 0.0
Axis Title
Axis Title

A 209.95
B -0.4784
DH 417.17065
KM (ap) 0 0.61977424
T2= 90°C
[AC](15mM) [AC](30mM)
(AU min^-1) (AU min^-1)
0.2 0.151
0.561 0.425
0.937 0.898

Inverso de T1=90°C
1/v1 1/v2 1/v3
2.35294118 5 6.62251656
0.999001 1.78253119 2.35294118
0.8605852 1.06723586 1.11358575

inverso t2=90°c
7

6 f(x) = 2.4904429546305 x + 0.806653528956522

R² = 0.996788897292802
5 1/v1
f(x) = 1.79885833622646 x + 0.77011780193661
R² = 0.989788122782159 Linear (1/v1)
4
Axis Title

1/v2
3 Linear (1/v2)
1/v3
2 f(x) = 0.699058997300536 x + 0.686613829353371 Linear (1/v3)
R² = 0.964789865987464
1

0
0 0.5 1 1.5 2 2.5
Axis Title

A B
0 0.6991 0.6866
15 1.7989 0.7701
30 2.4904 0.8067

[AC] Vmax(ap) Km(ap)

0 1.45645208 1.01820565103408
15 1.29853266 2.33593039864953
30 1.2396182 3.08714515929094

Inhibicion mixta tipo I

 [AC] 1/Vmax (ap) Km(ap)/Vmax (ap)
0 0.6866 0.6991
15 0.7701 1.7989
30 0.8067 2.4904

1/vmax(ap) y km(ap)/vmax(ap)

3
2.5
f(x) = 0.05971 x + 0.767150000000001 Column J
2 R² = 0.982976687507359
Linear (Column J)
Axis Title

1.5 Column K
Linear (Column K)
1
0.5 f(x) = 0.004003333333333 x + 0.694416666666667
R² = 0.95162677220351
0
0 5 10 15 20 25 30 35
Axis Title
<

vmax KINC KM KINC´ V 1/vmax(ap) km(ap)/vmax(

1.05241002 153.258065 1.18680278 9.39861523244313 0 0.6866 0.6991
15 0.7701 1.7989
30 0.8067 2.4904

ln KM ln Vmax ln KINC ln KINC´ 1/T°K

0.17126295 0.05108279 5.0321232 2.2405623627264 0.00309454
0.09969936 0.36470712 5.1567538 2.45371614294758 0.00275368

Ln Vmax Ln KINC
0.4 5.18
5.16
35 f(x) = − 920.110438230988 x + 2.89839963451522 f(x) = − 365.641029015904 x + 6.1636133066071
5.14
0.3 R² = 1 5.12 R² = 1
25 5.1
Axis Title

5.08 Colu
0.2 Column I 5.06
Linear (Column I) Line
15 5.04
5.02
0.1 5
05 4.98
4.96
0
0.0027 0.0028 0.0029 0.003 0.0031 0.0032
0.0027 0.0028 0.0029 0.003 0.0031 0.0032
Axis Title
Axis Title
05 4.98
4.96
0
0.0027 0.0028 0.0029 0.003 0.0031 0.0032
0.0027 0.0028 0.0029 0.003 0.0031 0.0032
Axis Title
Axis Title

A -920.11 A -365.64
B 2.8984 B 6.1636
Ea -1828.25857 Dhinc -726.52668
Vmax (ap) 0 18.14509 Kinc AP 0 475.135487
 90°C
1/vmax* KIN 1/VMAX km/vmax km/vmax*KI vmax KINC KM KINC´
0.004 0.6944 0.7672 0.0597 1.44009217 173.6 1.10483871 11.6314908

NC Ln KING´
2.5
2.45
x + 6.1636133066071 f(x) = − 625.350147427474 x + 4.17573224490951
2.4 R² = 1
2.35
Axis Title

Column J 2.3 Column K

Linear (Column J) 2.25 Linear (Column K)
2.2
2.15
2.1
1 0.0032 0.0026 0.0028 0.003 0.0032
Axis Title
 2.1
1 0.0032 0.0026 0.0028 0.003 0.0032
Axis Title

A -625.35
B 4.1757
DH -1242.57045
kinc´(ap) 0 65.0853835
1

mn K
r (Column K)