Example 4.

Time (hr) Substrate (mg/l) *Estimate the values of ko dan Km by the method of Gates an
0 19
1 16.5
2 13
3 9
4 5.5
5 2

Solution: ln(1+a(S° - S))/t

t (hr) S (ppm) S° - S - (1/t)ln(S/S°) a = 0.05 a = 0.1 a = 0.15
0 19 0
1 16.5 2.5 0.1411 0.1178 0.2231 0.3185
2 13 6 0.1897 0.1312 0.2350 0.3209
3 9 10 0.2491 0.1352 0.2310 0.3054
4 5.5 13.5 0.3099 0.1290 0.2136 0.2767
5 2 17 0.4503 0.1230 0.1987 0.2534

0.5000

0.4500

0.4000 f(x) = − 6.96273059961994 x + 1.80183318356976

0.3500

0.3000
- (1/t)ln(S/S°)

0.2500

0.2000

0.1500

0.1000

0.0500

0.0000
0.1000 0.1500 0.2000 0.2500 0.3000 0.3500 0.4000
ln(1+a(S° - S))/t

a=0.05 Linear (a=0.05) a=0.1

Linear (a=0.1) a=0.15 Linear (a=0.15)
the method of Gates and Marlar.

Slope,c : 6.9627
Intercept,b : 1.8018
ko = b/c-1 = 1.8018/(6.9627-1) = 0.302179 hr-1
Km = (1/a) + S°/c-1 = 10+19/(6.9627-1) = 4.863569 mg/l

500 0.4000
 Example 4.2

Ө (hr) S° (mg COD/L) S (mg COD/L) X (mg/l as MLSS) *Determine the values of ko,
11.2 1037 47 409
9 1010 58 401
6 987 80 388
3.75 986 162 358 12
3.4 1040 220 359
2.8 1043 303 324
10
f(x
R²
Solution:
8
assume Y: 0.445
Ө X (S°-S) t.X/Y(S°-S) 1/S
11.2 409 990 10.398 0.0213 6
9 401 952 8.519 0.0172
6 388 907 5.768 0.0125 4
3.75 358 824 3.661 0.0062
3.4 359 820 3.345 0.0045 2
2.8 324 740 2.755 0.0033

0
0.0000 0
*Determine the values of ko, Km, Y, and kd for this system.

12

10
f(x) = 419.827969658941 x + 1.1903027716126
R² = 0.986766070409801

0
0.0000 0.0050 0.0100 0.0150 0.0200 0.0250
 Example 4.3

T (°C) ko (hr-1) *Determine the E for this system.

22 0.34
27 0.56
32 0.73
37 1.05
42 0.81

Solution:
T(K) T (°C) 1/T ko ln ko 1/T*1000
295.15 22 0.003388 0.34 -1.0788 3.388
300.15 27 0.003332 0.56 -0.5798 3.332
305.15 32 0.003277 0.73 -0.3147 3.277
310.15 37 0.003224 1.05 0.0488 3.224
315.15 42 0.003173 0.81 -0.2107 3.173

Chart Title
0.
0.0 f(x)0.= − 4441.35241745747
0.0 0.
x + 14.13542165664460.0 0. 0.0
R² = 0.776595231782665

-0.

-0.

-0.

-0.

-1.

-1.
Slope: E/R = 4441.4
E = 4441.4 x R = 8825.062 cal/gmol
Problem 4-3