Professional Documents
Culture Documents
A. Define of Disinfection
Disinfection describes a process that eliminates many or all pathogenic
microorganisms. Disinfection does not destroy bacterial spores. Disinfectants are used on
inanimate objects in contrast to antiseptics, which are used on living tissue. Disinfection
usually involves chemicals, heat or ultraviolet light. The nature of chemical disinfection
varies with the type of product used.
Any process to destroy or prevent the growth of Microbes. Intended to inactivate
the microbes by physical, chemical or biological processes. Inactivation is achieved by
altering or destroying essential structures or functions within the microbe.Inactivation
processes include denaturation of:
- proteins (structural proteins, transport proteins, enzymes)
- nucleic acids (genomic DNA or RNA, mRNA, tRNA, etc)
- lipids (lipid bilayer membranes, other lipids)
e. PPE’s (Boots)
- If visibly soiled, soak in mild detergent to remove excessive dirt and
organic material then soak with 0.5% Sodium Hypochlorite solution
and allow to stand for 10 minutes
- If not, spray with 0.5% Sodium Hypochlorite solution and allow to
stand for 10 minutes
- Rinse off hypochlorite deposit with 70% alcohol
- Allow to dry.