CITRATE BUFFER; PH 3.0–6.2, PKA = 6.

40 Citrate buffer (Gomori, 1955)

stock solutions: A: 0.1 M citric acid; B: 0.1 M sodium citrate. Use x ml A + y ml
B and dilute to 100 ml with 50 ml DI.

0.1 M citric 0.1 M pH

acid sodium
citrate
46.5 3.5 3.0
43.7 6.3 3.2
40.0 10.0 3.4
37.0 13.0 3.6
35.0 15.0 3.8
33.0 17.0 4.0
31.5 18.5 4.2
28.0 22.0 4.4
25.5 24.5 4.6
23.0 27.0 4.8
20.5 29.5 5.0
18.0 32.0 5.2
16.0 34.0 5.4
13.7 36.3 5.6
11.8 38.2 5.8
9.5 41.5 6.0
7.2 42.8 6.2

PHOSPHATE–CITRATE BUFFER; PH 2.2–8.0, PK A = 7.20/6.40

Add the following to create 100 ml of phosphate/citrate buffer solution. Stock

solutions are
0.2 M dibasic sodium phosphate; 0.1 M citric acid (Pearse, 1980).
0.2 M 0.1 M citrate pH
Na2HPO4 (ml)
(ml)
5.4 44.6 2.6
7.8 42.2 2.8
10.2 39.8 3.0
12.3 37.7 3.2
14.1 35.9 3.4
 16.1 33.9 3.6
17.7 32.3 3.8
19.3 30.7 4.0
20.6 29.4 4.2
22.2 27.8 4.4
23.3 26.7 4.6
24.8 25.2 4.8
25.7 24.3 5.0
26.7 23.3 5.2
27.8 22.2 5.4
29.0 21.0 5.6
30.3 19.7 5.8
32.1 17.9 6.0
33.1 16.9 6.2
34.6 15.4 6.4
36.4 13.6 6.6
40.9 9.1 6.8
43.6 6.5 7.0

SODIUM ACETATE; PH 3.6–5.6, PKA = 4.76

Combine the following proportions of 0.1N acetic acid and 0.1N sodium acetate
(Pearse, 1980).
acetic acid sodium pH
acetate
185 15 3.6
176 24 3.8
164 36 4.0
147 53 4.2
126 74 4.4
102 98 4.6
80 120 4.8
59 141 5.0
42 158 5.2
29 171 5.4
19 181 5.6