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Cytology 2 Finish
Cytology 2 Finish
By :
Name : Pratiwi Kusuma Kurniawa
Student Number : B1B017007
Section : D1
Group :5
Assistant : Rahmi Mutia Mawardi
LABORATORY REPORT
PLANT STRUCTURE AND DEVELOPMENT II
2018
I. INTRODUCTION
Plant cytology is the study of the shape, composition, physical and chemical
properties of cells, as well as the development of cell wall. Plant cells are defined as
the universal basic unit of an organic structure. The structure that distinguishes plant
cells from animal cells is the presence of a cell wall which is the outermost layer of
cells bordering the plasma membrane. The cell wall will give the shape of a plant
cell. The cell contents are separated by the presence of cell walls. In high-level plants
there are various types of cells with variations in function, structure, and composition
with varying cell wall structure complexity. The constituent components of plant
cells can be divided into two groups, namely non-protoplasmic components or non-
living components (Setiowati, 2007).
Protoplast is stated, that a cell is said to die if the cell's lumen is no longer
contained protoplast. In protoplasm contained protoplasm, namely living substance.
Thus, "non- protoplasmic objects in cells" means objects that are without living
substance, which means also inanimate objects (Sumardi, 1993).
In plant cells there are many non-protoplasmic objects, which are usually in
vacuoles, in cell plasma and often in plastids. This non-protoplasmic object consists
of organic or inorganic substances, can be liquid or solid. According to botanists,
these non-protoplasmic objects are generally food reserves and are often found in
large quantities in reserves of reserve food reserves, such as in roots, tubers, fruit,
seeds and others. Non-protoplasmic objects are usually found in vacuoles, namely
cavities in the cytoplasm that borders on tonoplasm. This vacuole has a purpose for
regulating turgor stress, for the benefit of metabolic activities, and as a place of
stockpiling of unused materials, which is the end result of metabolism. Some of the
ergas are known for their functions, some are unknown (Sutrian, 2004).
II. OBJECTIVES
The objectives of Cytology II laboratory activity is observing of non-living
substances, such as Ca-oxalate and amylum.
III. MATERIALS AND METHODS
A. Materials
Equipments used in Cytology II laboratory activity are microscope, object
glass, cover glass, temporary report, razor, pipette, and light microscope.
Objects used in Cytology II laboratory activity are longitudinal section of
costa Carica papaya (papaya), cross section of petiole Colocasia esculenta (taro),
starch of Solanum tuberosum (potato), and starch of Zea mays (corn).
B. Methods
Methods used on Cytology II laboratory activity are:
1. A thin longitudinal section of costa Carica papaya are made, the placed it on
the center of clean objects glass and place a small drops of water, and covered
with cover glass,
2. A thin cross section of petiole Colocasia esculenta are made, then placed on
the center of clean objects glass then place a small drops of water, and covered
with cover glass,
3. Each starch of Solanum tuberosum and Zea mays are taken, the fluid that come
out (starch grains) are placed on the object glass, place a small drops of water,
and covered with cover glass,
4. Starting with a 45 angle, gently lower a cover slip into the microscope slide,
5. Observed under the microscope, starting with the lowest magnification (40x)
and switching to the next higher power objective,
6. A sketch of cells are drawn, and give some description.
Description:
1. Ca oxalate crystal
raphides shape
Description:
1. Hilum
2. Lamella
3. Amylum
Konyar, S., Necla, Ö. & Feruzan, D., 2014. Occurrence, Types and Distribution of
Calcium Oxalate Crystals in Leaves and Stems of Some Species of Poisonous
Plants. Botanical Study a Springer Journal, 55(32), pp. 1-9.
Melisa, A., 2018. Pemberian Kombinasi 2,4-D dan Kinetin Terhadap Induksi
Protocorm Like Bodies (Plb) Anggrek Grammatophyllum Scriptum Secara In
Vitro. Journal of Biology Education, 1(1), pp. 34-46.
Rahul, G., & Kadam, V., 2017. Histochemical Investigation of Cassia tora Linn.
World Journal of Pharmaceutical Research, 6(3), pp. 1008- 1015.
Venugopal, S., 2016. Biology: A Text Book of Biology. India: Saraswati House Pvt
Ltd.