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Recta de Calibrado

C (µg/ml) A (595nm) Muestras A (595nm) Concentración


0 0 Leche desnatada 0.083 0.0166050065
1 0.032 Leche semi- desnatada 0.117 10.4253412573
4 0.064 Leche entera 0.101 0.0167719653
6 0.079
8 0.09
10 0.099

Recta de Calibrado
0.12

0.1 f(x) = 0.0092754881x + 0.015835141

0.08
1; 0.083
0.06

0.04

0.02

0 0.5 1
0 1 2 3 4 5 6 7 8 9 10
0.0158 10.6236559
0.0251 14.2795699
0.053 12.5591398
0.0716
0.0902 100
0.1088
0.0158

A (595nm)
A (595nm)

2; 0.117
3; 0.101

1; 0.083

0.5 1 1.5 2 2.5 3 3.5


Tubo pH A (640nm)
1 3.03 0.277
2 3.53 0.358 Absorbancia/pH
3 3.76 0.538
0.9
4 3.96 0.703
0.8
5 4.15 0.811 0.7
6 4.5 0.525 0.6

Absorbancia
7 4.66 0.581 0.5 f(x) = - 0.0842839899x + 0.8361909997
R² = 0.1120627237
8 5.09 0.5 0.4
9 5.33 0.32 0.3
10 5.9 0.048 0.2
0.1
0
2.5 3 3.5 4 4.5 5 5.5
pH
rbancia/pH

899x + 0.8361909997
7

4 4.5 5 5.5 6
pH

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