Faculty of Seience Final Exam: May 2018 Chemistry Department Subject: Analytical Chemistry (6) Bachelor Students 3) Dr, Aimed Abdullah Time, Macks: 40 min, 35 Marks Course Code: C426 AC QL: Give short note about (1S Marks) | A) Advantages of TLC over paper chromatography. ‘ ‘b) Factors influence mobile phase selection in polarity based HPLC. |e: ‘The following questions regard the chromatograms shown below: (3 Marks) — )) Which column has grester efficiency? a Vb) Which column gives a greater selectivity factor (for X and Y)? c) Which column gives greater resolution between XandY?, Re Q3: Choose the correct answer (15 Marks) (1. The compound eluted first and retained less in RPLC is AX polar B. nonpolar C. ionic D, (B) and (C) ie .»- decreases the retention of the later-eluting components so that they are eluted faster A. Gradient elution —-B, Frontal analysis C. Isocraticelution D. None of these \, 43. Inplanar chromatograpgy include the capillary force and the solubility force of the solvent. < GReterding forcey B. propelling forces C. physical forces D. chemical forces 4. Chromatography is a physical method of separation in which the components to be separated are distributed between......... phase (5) Avone Bo C. three D. four Vv 5. Two polar aromatic compounds could be hardly separated by HPLC on silica stationary phase using heptane/IPA (80/20, V/V). If heptane percentage was decreased, the separation will improve B. decrease C. not change Good Luck Dr. Abed Abdullah\ 2 Faculty of Science Wid Final Exam: May 201% i Chemistry Department O) 2 Subject: Analytical Chemistry (6) Bachelor Students Dr. Ahmed Abdullah Time allowed: 35 min Course Code: 2d E)¥57.4) QI: Give short note about (20 Marks) he a cos ela Bo wo uC ‘According to the rate theory, what are the factors that lead to band broadening? fete ve- gael a (Advantages of SFE over the traditional solvent extraction techniques (77 The two method used to identify a specific component in HPLC chromatography indo qi lh Q2: Use the following gas chromatogram of a mixture of dioxane and cyclohexane eluted 0 ( non-polai column to answer the following two questions (3 Marks) | OO ‘me > UIE gf 1 e reasons behind the following (15 Marks) 1. In SFC, polar modifiers are added to CO2 mobile phase. * * 2, In HPLC, silica-based stationary phases are more widely used. _ 3: When choosing a solvent. be aware of its absorbance cutoff value. Q2: Diseuss briefly the basic operations involved in a chromatugraphic technique (10 Marks) Q3: Choose the correct answer (10 Marks) . & 1) A mixture of benzene and methane (Dead time marker) was injected info a gas chromatograph. Methane gave a sharp spike at 32.0 s, whereas benzene required 257 s. The adjusted retention times of benzene is 2.225 b.300s ©. 289s . None of these 2) a quantitative measure of how well two elution peaks are separated a. Resolution (Rs) b. Retention time ¢ Injection point d. Retention factor 3) An injection of a sample with unknown concentration of benzene has a peak area of $7,000. What is the amount of benzene (ug/ml) present in the sample (given that the response factor. aszxio b. 1.89 ; é ©. 1149 d. None of these 4) A sample containing toluenc (1), phenol (2) and benzoic acid (3) is separated using NPLC, the expected order of elution is .. on a. (1),2).G) b.42). (1). 3) ©. 3).(1). 2) d. none of the above 5) In GC, If the carrier gas flow is high, the retention time but the quality of separation. a, shorter. increased b. shorter, decreased c. longer, decreased d. longer, decreased Good tuck, Dr. Atoned Abdullan